CSPI07G18450 (gene) Wild cucumber (PI 183967)

NameCSPI07G18450
Typegene
OrganismCucumis sativus (Wild cucumber (PI 183967))
DescriptionPectin lyase-like superfamily protein
LocationChr7 : 16486152 .. 16488019 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
CGAAGATTCAACCTTTCTTGTTTTGTGTTCATTCCATGTCAAAATAGCCGTCTTGGGGTTTGAGAAGAATCAAAAGAAATGTCTAGTTTTGTGTTGTTGTCTTTTGCAATTTGGTTATCTTTCGTTTCTTGTCTTCTTTCTTCTTCTCAAGCTAGATGGCATCATCACACTAAGCAAAAACACTCTCATCGCCATAGACATAGCTACATTTCCCACCCGCCTTCTCTTTCTCCTTCTCCTTCGGATTCGCCAGACGATGGATATTATATTAATGATACGAATATTTTCGACGTGAGAGCTTTTGGTGCTATTGGGGATGGCATTGTAGATGATACGGAGGCATTCAAGATGGCTTGGGACACGGCATGTCAGAGTGAGGATGACTCTGTAGTCATGCTCGTTCCCTATGGCTTCTCGTTTATGATTCAATCAACAATCTTCACTGGCCCTTGCCATGGTTACCTTATGTTACGAGTTGATGGCACGTTGATGACACCTGATGGCCCCGATGCTTGGCCGAAGAATGCTAGTCGTCGCCAATGGTTGGTCTTTTATAGGATTGATGGAATGTCCTTAGAAGGAGGAGGTGTGATTGATGGGAGAGGTCAAAAGTGGTGGGATCTTCCTTGCAAACCACACAAGGTATATTCAATCTTTTTCCATAAATTTCATTACTAGTATATCCTAACGCGATATATCTTGTGTAAAACTCAGGGACCAAATGGAACTACATTACCAGGACCGTGTGACAGCCCAATTGTAAGTTCATATATACACTCAAAGTTATATTGTACCTTTTTCTGCTATAAAAAATATGAAAAAATTACTTAGCATAACATTCTATTGATTTTAGGCAATAAGGTTCTTCATGAGCTCCAATTTGACTGTCCACGGACTTAAAATCACAAATAGCCCTCAATTCCACTTCAGATTCGACGGTTGCAAGACTGTTCATATCGACTCGATTCACATAAAATCTCCCGGTCTTAGTCCCAATACAGACGGAATCCATATCGAGAACACAAATGGTGTCCAGATATATAATTCAGTCATTAACAATGGTTAGTAAGTTGAACAAAAAATAACAATTAGCACCGATAATCACTTTGTTAAAGGAAATGGAATTTTAAATCATGCATATTTTCCATTATGTGTAGGGGATGATTGTGTATCAATTGGATCTGGTTCATATGACTTAGATATCAGAAACATCACTTGTGGACCAAGTCATGGAATCAGGTATTGTGTTAATTAGGCATTTTAGTTACGTTGCCAATTTGAATTATATATATATCAGCTCAACTAGTTAAGACATTAGTAATTATTAGTTCCTAAACGTTTTTTCTTTATCCTTCTGTCCCAGTATTGGAAGTCTCGGCGTTCGCAACTCTAGAGCTTGCGTTACGAACATCACAGTTAGGGACTCAGTAATCAAAGACTCAGACAATGGAGTTAGGATCAAGACATGGCAAGGTGGATATGGAGCAGTAAGAGGAATAACATTTAGCAACATTCACATGGACAATGTTAGAAATCCCATCATTATAGATCAATTCTATTGCTTAACAAAGGATTGCACCAATCAAACCTCAGCAGTTTCAGTATCAGATATTCAATACACAAACATCAAAGGAACATACGACATTCGGAGCCCTCCTATGCACTTCGCTTGCAGCGACTCGGTCCCTTGTACCAATCTAACATTGTCGGAGATCGAGCTACTTCCCGCTCAAGGAGATATAGTGTTGGACCCTTTTTGTTGGAATGCTTATGGAGATCTTCAAACCCTTACCATTCCCCCTGTCTCATGCTTGCAAGAGGGACTTCCTTCTTCTGTCAACTTTAACAATGACAATGAGTACTGTTGA

mRNA sequence

ATGTCTAGTTTTGTGTTGTTGTCTTTTGCAATTTGGTTATCTTTCGTTTCTTGTCTTCTTTCTTCTTCTCAAGCTAGATGGCATCATCACACTAAGCAAAAACACTCTCATCGCCATAGACATAGCTACATTTCCCACCCGCCTTCTCTTTCTCCTTCTCCTTCGGATTCGCCAGACGATGGATATTATATTAATGATACGAATATTTTCGACGTGAGAGCTTTTGGTGCTATTGGGGATGGCATTGTAGATGATACGGAGGCATTCAAGATGGCTTGGGACACGGCATGTCAGAGTGAGGATGACTCTGTAGTCATGCTCGTTCCCTATGGCTTCTCGTTTATGATTCAATCAACAATCTTCACTGGCCCTTGCCATGGTTACCTTATGTTACGAGTTGATGGCACGTTGATGACACCTGATGGCCCCGATGCTTGGCCGAAGAATGCTAGTCGTCGCCAATGGTTGGTCTTTTATAGGATTGATGGAATGTCCTTAGAAGGAGGAGGTGTGATTGATGGGAGAGGTCAAAAGTGGTGGGATCTTCCTTGCAAACCACACAAGGGACCAAATGGAACTACATTACCAGGACCGTGTGACAGCCCAATTGCAATAAGGTTCTTCATGAGCTCCAATTTGACTGTCCACGGACTTAAAATCACAAATAGCCCTCAATTCCACTTCAGATTCGACGGTTGCAAGACTGTTCATATCGACTCGATTCACATAAAATCTCCCGGTCTTAGTCCCAATACAGACGGAATCCATATCGAGAACACAAATGGTGTCCAGATATATAATTCAGTCATTAACAATGGGGATGATTGTGTATCAATTGGATCTGGTTCATATGACTTAGATATCAGAAACATCACTTGTGGACCAAGTCATGGAATCAGTATTGGAAGTCTCGGCGTTCGCAACTCTAGAGCTTGCGTTACGAACATCACAGTTAGGGACTCAGTAATCAAAGACTCAGACAATGGAGTTAGGATCAAGACATGGCAAGGTGGATATGGAGCAGTAAGAGGAATAACATTTAGCAACATTCACATGGACAATGTTAGAAATCCCATCATTATAGATCAATTCTATTGCTTAACAAAGGATTGCACCAATCAAACCTCAGCAGTTTCAGTATCAGATATTCAATACACAAACATCAAAGGAACATACGACATTCGGAGCCCTCCTATGCACTTCGCTTGCAGCGACTCGGTCCCTTGTACCAATCTAACATTGTCGGAGATCGAGCTACTTCCCGCTCAAGGAGATATAGTGTTGGACCCTTTTTGTTGGAATGCTTATGGAGATCTTCAAACCCTTACCATTCCCCCTGTCTCATGCTTGCAAGAGGGACTTCCTTCTTCTGTCAACTTTAACAATGACAATGAGTACTGTTGA

Coding sequence (CDS)

ATGTCTAGTTTTGTGTTGTTGTCTTTTGCAATTTGGTTATCTTTCGTTTCTTGTCTTCTTTCTTCTTCTCAAGCTAGATGGCATCATCACACTAAGCAAAAACACTCTCATCGCCATAGACATAGCTACATTTCCCACCCGCCTTCTCTTTCTCCTTCTCCTTCGGATTCGCCAGACGATGGATATTATATTAATGATACGAATATTTTCGACGTGAGAGCTTTTGGTGCTATTGGGGATGGCATTGTAGATGATACGGAGGCATTCAAGATGGCTTGGGACACGGCATGTCAGAGTGAGGATGACTCTGTAGTCATGCTCGTTCCCTATGGCTTCTCGTTTATGATTCAATCAACAATCTTCACTGGCCCTTGCCATGGTTACCTTATGTTACGAGTTGATGGCACGTTGATGACACCTGATGGCCCCGATGCTTGGCCGAAGAATGCTAGTCGTCGCCAATGGTTGGTCTTTTATAGGATTGATGGAATGTCCTTAGAAGGAGGAGGTGTGATTGATGGGAGAGGTCAAAAGTGGTGGGATCTTCCTTGCAAACCACACAAGGGACCAAATGGAACTACATTACCAGGACCGTGTGACAGCCCAATTGCAATAAGGTTCTTCATGAGCTCCAATTTGACTGTCCACGGACTTAAAATCACAAATAGCCCTCAATTCCACTTCAGATTCGACGGTTGCAAGACTGTTCATATCGACTCGATTCACATAAAATCTCCCGGTCTTAGTCCCAATACAGACGGAATCCATATCGAGAACACAAATGGTGTCCAGATATATAATTCAGTCATTAACAATGGGGATGATTGTGTATCAATTGGATCTGGTTCATATGACTTAGATATCAGAAACATCACTTGTGGACCAAGTCATGGAATCAGTATTGGAAGTCTCGGCGTTCGCAACTCTAGAGCTTGCGTTACGAACATCACAGTTAGGGACTCAGTAATCAAAGACTCAGACAATGGAGTTAGGATCAAGACATGGCAAGGTGGATATGGAGCAGTAAGAGGAATAACATTTAGCAACATTCACATGGACAATGTTAGAAATCCCATCATTATAGATCAATTCTATTGCTTAACAAAGGATTGCACCAATCAAACCTCAGCAGTTTCAGTATCAGATATTCAATACACAAACATCAAAGGAACATACGACATTCGGAGCCCTCCTATGCACTTCGCTTGCAGCGACTCGGTCCCTTGTACCAATCTAACATTGTCGGAGATCGAGCTACTTCCCGCTCAAGGAGATATAGTGTTGGACCCTTTTTGTTGGAATGCTTATGGAGATCTTCAAACCCTTACCATTCCCCCTGTCTCATGCTTGCAAGAGGGACTTCCTTCTTCTGTCAACTTTAACAATGACAATGAGTACTGTTGA
BLAST of CSPI07G18450 vs. Swiss-Prot
Match: PGLR4_ARATH (Polygalacturonase At1g48100 OS=Arabidopsis thaliana GN=At1g48100 PE=2 SV=1)

HSP 1 Score: 560.1 bits (1442), Expect = 2.3e-158
Identity = 266/474 (56.12%), Postives = 343/474 (72.36%), Query Frame = 1

Query: 3   SFVLLSFAIWLSFVSCLLSSSQARWHHHTKQKHSHRHRHSYISHPPSLSPSPSD------ 62
           S +L+ + I         + ++AR+H+H K +H   H       PP  + +P D      
Sbjct: 5   SGILVIYIISTVLFGHFTNVARARYHYH-KGRHGVTHPLPPPPPPPLETANPPDQVPSDP 64

Query: 63  --SPDDGYYINDTN-IFDVRAFGAIGDGIVDDTEAFKMAWDTACQSEDDSVVMLVPYGFS 122
             SPD     +D+  +FDV +FGA+GDG  DDT AF+ AW  AC  E  S V+L P G  
Sbjct: 65  YPSPDPAPGDSDSGCVFDVTSFGAVGDGSCDDTAAFQDAWKAACAVE--SGVVLAPEGGV 124

Query: 123 FMIQSTIFTGPCHGYLMLRVDGTLMTPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVID 182
           F I STIF+GPC   L+ ++DG LM PDGP+ WP+  ++ QWLVFYR+DG +  G G ++
Sbjct: 125 FKITSTIFSGPCKPGLVFQLDGVLMPPDGPEEWPEKDNKNQWLVFYRLDGFTFSGKGTVE 184

Query: 183 GRGQKWWDLPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTVHGLKITNSPQFHFRFDGC 242
           G GQKWWDLPCKPH+GP+G++  GPC SP  IRFFMS+N+ V GL+I NSPQFH +FDGC
Sbjct: 185 GNGQKWWDLPCKPHRGPDGSSSSGPCASPTMIRFFMSNNIEVKGLRIQNSPQFHMKFDGC 244

Query: 243 KTVHIDSIHIKSPGLSPNTDGIHIENTNGVQIYNSVINNGDDCVSIGSGSYDLDIRNITC 302
           + V I+ I I SP LSPNTDGIH+ NT  V IYNSV++NGDDC+SIG+G  D+DI+ +TC
Sbjct: 245 QGVLINEIQISSPKLSPNTDGIHLGNTRSVGIYNSVVSNGDDCISIGTGCSDVDIQGVTC 304

Query: 303 GPSHGISIGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMD 362
           GPSHGISIGSLGV NS+ACV+NITVR++VI+DSDNG+R+KTWQGG G+V  + F NI M+
Sbjct: 305 GPSHGISIGSLGVHNSQACVSNITVRNTVIRDSDNGLRVKTWQGGTGSVSNLLFENIQME 364

Query: 363 NVRNPIIIDQFYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLT 422
           NV N II+DQ+YC +KDC N+TSAV V D++Y NIKGTYD+RSPP+HFACSD+V CTN+T
Sbjct: 365 NVLNCIIVDQYYCQSKDCRNETSAVKVFDVEYRNIKGTYDVRSPPIHFACSDTVACTNIT 424

Query: 423 LSEIELLPAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSVNFNNDNEYC 468
           +SE+ELLP +G++V DPFCWNAYG  +TLTIPP+ CL +G P      + N  C
Sbjct: 425 MSEVELLPEEGELVDDPFCWNAYGKQETLTIPPIDCLLDGSPVVEEAYDSNYGC 475

BLAST of CSPI07G18450 vs. Swiss-Prot
Match: PGLR5_ARATH (Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana GN=At1g80170 PE=1 SV=1)

HSP 1 Score: 340.9 bits (873), Expect = 2.2e-92
Identity = 168/386 (43.52%), Postives = 235/386 (60.88%), Query Frame = 1

Query: 69  IFDVRAFGAIGDGIVDDTEAFKMAWDTACQSEDDSVVMLVPYGFSFMIQSTIFTGPCHGY 128
           +  V  FGA G+G+ DDT+AF  AW TAC S+  + + LVP  ++ +++    +GPC   
Sbjct: 52  LLHVGNFGAKGNGVTDDTKAFADAWKTACSSKVKTRI-LVPENYTCLLRPIDLSGPCKAR 111

Query: 129 LMLRVDGTLMTPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGRGQKWWDLPCKPHK 188
           L L++ GT++ P+ PD W +  +RR+WL F+ +  +++EGGG ++G GQ+WW+  CK H 
Sbjct: 112 LTLQISGTIIAPNDPDVW-EGLNRRKWLYFHGLSRLTVEGGGTVNGMGQEWWERSCK-HN 171

Query: 189 GPNGTTLPGPC-DSPIAIRFFMSSNLTVHGLKITNSPQFHFRFDGCKTVHIDSIHIKSPG 248
             N      PC  +P A+ F    N+ V  L + +S Q H     C+ V I  + + +P 
Sbjct: 172 HSN------PCRGAPTALTFHKCKNMRVENLNVIDSQQMHIALTSCRRVTISGLKVIAPA 231

Query: 249 LSPNTDGIHIENTNGVQIYNSVINNGDDCVSIGSGSYDLDIRNITCGPSHGISIGSLGVR 308
            SPNTDGIHI  + G+ I N+ ++ GDDC+SI   S  + I NI CGP HGISIGSLG  
Sbjct: 232 TSPNTDGIHISVSRGIVIDNTTVSTGDDCISIVKNSTQISISNIICGPGHGISIGSLGKS 291

Query: 309 NSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPIIIDQFYC- 368
            S   V +ITV  ++I D+ NGVRIKTWQGG G V  I F NI M+NV NPIIIDQ+YC 
Sbjct: 292 KSWEEVRDITVDTAIISDTANGVRIKTWQGGSGLVSKIIFRNIKMNNVSNPIIIDQYYCD 351

Query: 369 LTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIELLPAQGDI 428
             K C NQTSA+S+ +I + +++GT       +  +CSDS PC N+ L +I+L P+ GD 
Sbjct: 352 SRKPCANQTSAISIENISFVHVRGT-SASKEAIKISCSDSSPCRNILLQDIDLEPSNGDG 411

Query: 429 VLDPFCWNAYGDLQTLTIPPVSCLQE 453
             + FCW AYG       PP  CL +
Sbjct: 412 FTESFCWEAYGSSSGQVYPP-PCLSD 426

BLAST of CSPI07G18450 vs. Swiss-Prot
Match: PGLR2_CRYJA (Polygalacturonase OS=Cryptomeria japonica PE=1 SV=1)

HSP 1 Score: 310.8 bits (795), Expect = 2.5e-83
Identity = 159/360 (44.17%), Postives = 214/360 (59.44%), Query Frame = 1

Query: 68  NIFDVRAFGAIGDGIVDDTEAFKMAWDTACQSEDDSVVMLVPYGFSFMIQSTIFTGPCHG 127
           NIF+V  +GA+GDG  D TEAF  AW  AC+    S ++LVP    F++ +  F GPC  
Sbjct: 57  NIFNVEKYGAVGDGKHDCTEAFSTAWQAACKKP--SAMLLVPGNKKFVVNNLFFNGPCQP 116

Query: 128 YLMLRVDGTLMTPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGRGQKWWDLPCKPH 187
           +   +VDG +     P +W  N   R WL F ++ G +L G GVIDG+G++WW   CK  
Sbjct: 117 HFTFKVDGIIAAYQNPASWKNN---RIWLQFAKLTGFTLMGKGVIDGQGKQWWAGQCK-- 176

Query: 188 KGPNGTTLPGPCDSPIAIRFFMSSNLTVHGLKITNSPQFHFRFDGCKTVHIDSIHIKSPG 247
              NG  +    D P AI+F  S+ L + GLK+ NSP+FH  F  C+ V I  I I +P 
Sbjct: 177 -WVNGREICNDRDRPTAIKFDFSTGLIIQGLKLMNSPEFHLVFGNCEGVKIIGISITAPR 236

Query: 248 LSPNTDGIHIENTNGVQIYNSVINNGDDCVSIGSGSYDLDIRNITCGPSHGISIGSLGVR 307
            SPNTDGI I  +    +  + I  GDDCV+IG+GS ++ I ++ CGP HGISIGSLG  
Sbjct: 237 DSPNTDGIDIFASKNFHLQKNTIGTGDDCVAIGTGSSNIVIEDLICGPGHGISIGSLGRE 296

Query: 308 NSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPIIIDQFYCL 367
           NSRA V+ + V  +   D+ NG+RIKTWQGG G    I + N+ M N  NPI+I+QFYC 
Sbjct: 297 NSRAEVSYVHVNGAKFIDTQNGLRIKTWQGGSGMASHIIYENVEMINSENPILINQFYCT 356

Query: 368 TKD-CTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIELLPAQGDI 427
           +   C NQ SAV + D+ Y NI+GT    +  +   CSDS+PC ++ LS+I L    G I
Sbjct: 357 SASACQNQRSAVQIQDVTYKNIRGT-SATAAAIQLKCSDSMPCKDIKLSDISLKLTSGKI 407

BLAST of CSPI07G18450 vs. Swiss-Prot
Match: PGLR2_CHAOB (Polygalacturonase OS=Chamaecyparis obtusa PE=1 SV=1)

HSP 1 Score: 304.7 bits (779), Expect = 1.8e-81
Identity = 158/357 (44.26%), Postives = 215/357 (60.22%), Query Frame = 1

Query: 69  IFDVRAFGAIGDGIVDDTEAFKMAWDTACQSEDDSVVMLVPYGFSFMIQSTIFTGPCHGY 128
           +F+V  +GA+GDG  D TEAF   W+ AC+    S V+LVP    F + + +F GPC  +
Sbjct: 58  VFNVEQYGAVGDGKHDSTEAFATTWNAACKKA--SAVLLVPANKKFFVNNLVFRGPCQPH 117

Query: 129 LMLRVDGTLMTPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGRGQKWWDLPCKPHK 188
           L  +VDGT++    P  W KN+  + WL F ++   +L G GVIDG+GQ+WW   CK   
Sbjct: 118 LSFKVDGTIVAQPDPARW-KNS--KIWLQFAQLTDFNLMGTGVIDGQGQQWWAGQCKV-- 177

Query: 189 GPNGTTLPGPCDSPIAIRFFMSSNLTVHGLKITNSPQFHFRFDGCKTVHIDSIHIKSPGL 248
             NG T+    + P AI+   S ++TV  L + NSP+FH  F  C+ V I  + IK+P  
Sbjct: 178 -VNGRTVCNDRNRPTAIKIDYSKSVTVKELTLMNSPEFHLVFGECEGVKIQGLKIKAPRD 237

Query: 249 SPNTDGIHIENTNGVQIYNSVINNGDDCVSIGSGSYDLDIRNITCGPSHGISIGSLGVRN 308
           SPNTDGI I  +    I   VI  GDDC++IG+GS ++ I+++ CGP HGISIGSLG  N
Sbjct: 238 SPNTDGIDIFASKRFHIEKCVIGTGDDCIAIGTGSSNITIKDLICGPGHGISIGSLGRDN 297

Query: 309 SRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPIIIDQFYCLT 368
           SRA V+++ V  +   D+ NG+RIKTWQGG G    IT+ N+ M N  NPI+I+QFYC +
Sbjct: 298 SRAEVSHVHVNRAKFIDTQNGLRIKTWQGGSGLASYITYENVEMINSENPILINQFYCTS 357

Query: 369 KD-CTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIELLPAQG 425
              C NQ SAV +  + Y NI GT    +  +   CSDSVPCT + LS + L    G
Sbjct: 358 ASACQNQRSAVQIQGVTYKNIHGT-SATAAAIQLMCSDSVPCTGIQLSNVSLKLTSG 405

BLAST of CSPI07G18450 vs. Swiss-Prot
Match: PGLR2_JUNAS (Polygalacturonase OS=Juniperus ashei GN=JNA2 PE=1 SV=1)

HSP 1 Score: 299.3 bits (765), Expect = 7.4e-80
Identity = 154/359 (42.90%), Postives = 214/359 (59.61%), Query Frame = 1

Query: 69  IFDVRAFGAIGDGIVDDTEAFKMAWDTACQSEDDSVVMLVPYGFSFMIQSTIFTGPCHGY 128
           +F+V  +GA+GDG  D T+AF+  W+ AC     S V LVP    F++ + +F GPC  +
Sbjct: 59  VFNVEHYGAVGDGKHDSTDAFEKTWNAACNKL--SAVFLVPANKKFVVNNLVFYGPCQPH 118

Query: 129 LMLRVDGTLMTPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGRGQKWWDLPCKPHK 188
              +VDGT+     P  W KN+  + W+ F R+   +L G GVIDG+G +WW   CK   
Sbjct: 119 FSFKVDGTIAAYPDPAKW-KNS--KIWMHFARLTDFNLMGTGVIDGQGNRWWSDQCKTI- 178

Query: 189 GPNGTTLPGPCDSPIAIRFFMSSNLTVHGLKITNSPQFHFRFDGCKTVHIDSIHIKSPGL 248
             NG T+      P AI+   S ++TV  L +TNSP+FH  F  C  V I  I IK+P  
Sbjct: 179 --NGRTVCNDKGRPTAIKIDFSKSVTVKELTLTNSPEFHLVFGECDGVKIQGIKIKAPRD 238

Query: 249 SPNTDGIHIENTNGVQIYNSVINNGDDCVSIGSGSYDLDIRNITCGPSHGISIGSLGVRN 308
           SPNTDGI I  +   +I    I  GDDCV++G+GS ++ I+++TCGP HG+SIGSLG  N
Sbjct: 239 SPNTDGIDIFASKRFEIEKCTIGTGDDCVAVGTGSSNITIKDLTCGPGHGMSIGSLGKGN 298

Query: 309 SRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPIIIDQFYCLT 368
           SR+ V+ + +  +   D+ NG+RIKTWQGG G    IT+ N+ M N  NPI+I+QFYC +
Sbjct: 299 SRSEVSFVHLDGAKFIDTQNGLRIKTWQGGSGLASHITYENVEMINAENPILINQFYCTS 358

Query: 369 -KDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIELLPAQGDI 427
              C NQ SAV + D+ + NI GT    +  +   CSDSVPC+N+ LS + L    G +
Sbjct: 359 AAACKNQRSAVKIQDVTFKNIHGT-SATTAAIQLMCSDSVPCSNIKLSNVFLKLTSGKV 408

BLAST of CSPI07G18450 vs. TrEMBL
Match: A0A0A0KBA7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G414430 PE=3 SV=1)

HSP 1 Score: 987.3 bits (2551), Expect = 6.6e-285
Identity = 467/467 (100.00%), Postives = 467/467 (100.00%), Query Frame = 1

Query: 1   MSSFVLLSFAIWLSFVSCLLSSSQARWHHHTKQKHSHRHRHSYISHPPSLSPSPSDSPDD 60
           MSSFVLLSFAIWLSFVSCLLSSSQARWHHHTKQKHSHRHRHSYISHPPSLSPSPSDSPDD
Sbjct: 1   MSSFVLLSFAIWLSFVSCLLSSSQARWHHHTKQKHSHRHRHSYISHPPSLSPSPSDSPDD 60

Query: 61  GYYINDTNIFDVRAFGAIGDGIVDDTEAFKMAWDTACQSEDDSVVMLVPYGFSFMIQSTI 120
           GYYINDTNIFDVRAFGAIGDGIVDDTEAFKMAWDTACQSEDDSVVMLVPYGFSFMIQSTI
Sbjct: 61  GYYINDTNIFDVRAFGAIGDGIVDDTEAFKMAWDTACQSEDDSVVMLVPYGFSFMIQSTI 120

Query: 121 FTGPCHGYLMLRVDGTLMTPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGRGQKWW 180
           FTGPCHGYLMLRVDGTLMTPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGRGQKWW
Sbjct: 121 FTGPCHGYLMLRVDGTLMTPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGRGQKWW 180

Query: 181 DLPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTVHGLKITNSPQFHFRFDGCKTVHIDS 240
           DLPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTVHGLKITNSPQFHFRFDGCKTVHIDS
Sbjct: 181 DLPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTVHGLKITNSPQFHFRFDGCKTVHIDS 240

Query: 241 IHIKSPGLSPNTDGIHIENTNGVQIYNSVINNGDDCVSIGSGSYDLDIRNITCGPSHGIS 300
           IHIKSPGLSPNTDGIHIENTNGVQIYNSVINNGDDCVSIGSGSYDLDIRNITCGPSHGIS
Sbjct: 241 IHIKSPGLSPNTDGIHIENTNGVQIYNSVINNGDDCVSIGSGSYDLDIRNITCGPSHGIS 300

Query: 301 IGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPII 360
           IGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPII
Sbjct: 301 IGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPII 360

Query: 361 IDQFYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIELL 420
           IDQFYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIELL
Sbjct: 361 IDQFYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIELL 420

Query: 421 PAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSVNFNNDNEYC 468
           PAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSVNFNNDNEYC
Sbjct: 421 PAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSVNFNNDNEYC 467

BLAST of CSPI07G18450 vs. TrEMBL
Match: A0A061DSI7_THECC (Pectin lyase-like superfamily protein OS=Theobroma cacao GN=TCM_004895 PE=3 SV=1)

HSP 1 Score: 740.7 bits (1911), Expect = 1.1e-210
Identity = 352/468 (75.21%), Postives = 395/468 (84.40%), Query Frame = 1

Query: 9   FAIWLSFVSCLLSSSQARWH-HHTKQKHSHRHRHSYISHPP--------SLSPSPSDSPD 68
           F   +SFV C   S+QARWH HHTK KH+H H  S IS PP         L+P PS   D
Sbjct: 9   FIFCMSFV-CFFLSTQARWHYHHTKHKHTHHHNISEISAPPITAPEPSSPLAPEPSSPSD 68

Query: 69  DGYYINDTNIFDVRAFGAIGDGIVDDTEAFKMAWDTACQSEDDSVVMLVPYGFSFMIQST 128
           DG   N T +FDVR FGA+GDG+ DDTEAFKMAWD+AC+   +S V+ VPYGFSFMIQST
Sbjct: 69  DG---NATGVFDVRKFGAVGDGVTDDTEAFKMAWDSACRV--NSAVLHVPYGFSFMIQST 128

Query: 129 IFTGPCHGYLMLRVDGTLMTPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGRGQKW 188
           IF GPC G L+ +VDGTLM PDGPDAWP+N S+RQWLVFYRI+GMSL+GGG+IDGRGQKW
Sbjct: 129 IFLGPCQGGLVFQVDGTLMPPDGPDAWPENTSKRQWLVFYRINGMSLQGGGLIDGRGQKW 188

Query: 189 WDLPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTVHGLKITNSPQFHFRFDGCKTVHID 248
           WDLPCKPHKG N T LPGPCDSPIA+RFFMSSNLTV GLKI +SP+FHFRFDGC+ VH++
Sbjct: 189 WDLPCKPHKGKNRTALPGPCDSPIALRFFMSSNLTVKGLKIKDSPKFHFRFDGCQNVHVE 248

Query: 249 SIHIKSPGLSPNTDGIHIENTNGVQIYNSVINNGDDCVSIGSGSYDLDIRNITCGPSHGI 308
           S+HI +P LSPNTDGIHIENTNGV+IYNSVI+NGDDC+SIGSG +D+DI+N+TCGP HGI
Sbjct: 249 SLHITAPALSPNTDGIHIENTNGVEIYNSVISNGDDCISIGSGCHDVDIKNLTCGPGHGI 308

Query: 309 SIGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPI 368
           SIGSLG  NSRACV+N+TVRDSVIK SDNGVRIKTWQGG GAV GITFSNIHM+ VRNPI
Sbjct: 309 SIGSLGNHNSRACVSNVTVRDSVIKVSDNGVRIKTWQGGSGAVSGITFSNIHMEAVRNPI 368

Query: 369 IIDQFYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIEL 428
           IIDQFYCLTK CTNQTSAV VSDI Y +IKGTYDIRSPPMHFACSDSVPCTN+TLS+IEL
Sbjct: 369 IIDQFYCLTKGCTNQTSAVYVSDILYESIKGTYDIRSPPMHFACSDSVPCTNITLSDIEL 428

Query: 429 LPAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSVNFNNDNEYC 468
           LPAQGDIVLDPFCWNAYGDL++LTIPPVSCL  G+P S+  N D  YC
Sbjct: 429 LPAQGDIVLDPFCWNAYGDLESLTIPPVSCLMAGIPRSILDNKDMGYC 470

BLAST of CSPI07G18450 vs. TrEMBL
Match: V7BKM1_PHAVU (Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_007G211400g PE=3 SV=1)

HSP 1 Score: 728.4 bits (1879), Expect = 5.5e-207
Identity = 346/472 (73.31%), Postives = 401/472 (84.96%), Query Frame = 1

Query: 6   LLSFAIWLSFVSCLLSSSQARWHHHTKQKHSHRHRHSYISHPPSLSPSPSDSPDDG---- 65
           +LS A+ L F++  L + QAR+H H K KHSH H    IS  PS  P PS +PD+     
Sbjct: 6   ILSLALSLYFLTLFLPT-QARYHFH-KNKHSHYHNAPEISPSPSPLPGPSSAPDEAPSPS 65

Query: 66  ------YYINDTNIFDVRAFGAIGDGIVDDTEAFKMAWDTACQSEDDSVVMLVPYGFSFM 125
                 Y+    N+FD+RAFGAIGDG+ DDTE+FKMAWDTACQSE    V+LVP+G SF+
Sbjct: 66  PTVDENYHNTSNNLFDIRAFGAIGDGVTDDTESFKMAWDTACQSESAVKVILVPHGLSFI 125

Query: 126 IQSTIFTGPCHGYLMLRVDGTLMTPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGR 185
           IQSTIFTGPC G L+L+VDGTLM PDGPD+WPKN SRRQWLVFYRI+GMSLEG G+IDGR
Sbjct: 126 IQSTIFTGPCKGGLVLKVDGTLMPPDGPDSWPKNNSRRQWLVFYRINGMSLEGSGLIDGR 185

Query: 186 GQKWWDLPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTVHGLKITNSPQFHFRFDGCKT 245
           G+KWWDLPCKPHKGP+GTT PGPCDSP+A+RFFMSSNLTV GLKI NSPQFHFRFDGC++
Sbjct: 186 GEKWWDLPCKPHKGPHGTTSPGPCDSPVALRFFMSSNLTVQGLKIKNSPQFHFRFDGCES 245

Query: 246 VHIDSIHIKSPGLSPNTDGIHIENTNGVQIYNSVINNGDDCVSIGSGSYDLDIRNITCGP 305
           VH++SI+I SP LSPNTDGIHIENTN V+IYNSVI+NGDDCVSIG+G +D+DI+NITCGP
Sbjct: 246 VHVESIYITSPALSPNTDGIHIENTNDVKIYNSVISNGDDCVSIGAGCHDVDIKNITCGP 305

Query: 306 SHGISIGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNV 365
            HGISIGSLG  NSRACV+NITVRDSVIK SDNGVRIKTWQGG G+V G+TF+NIHM++V
Sbjct: 306 GHGISIGSLGNHNSRACVSNITVRDSVIKVSDNGVRIKTWQGGSGSVSGVTFTNIHMESV 365

Query: 366 RNPIIIDQFYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTLS 425
           RNPIIIDQFYCL+KDC+N+TSAV VS+I YTNIKGTYDIR PPMHFACSDSVPCTNLTLS
Sbjct: 366 RNPIIIDQFYCLSKDCSNKTSAVFVSNISYTNIKGTYDIRHPPMHFACSDSVPCTNLTLS 425

Query: 426 EIELLPAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSVNFNNDNEYC 468
           +IELLP++GDIV DP+CW+AYG  QTLTIPPVSCL EGLP S++  ND ++C
Sbjct: 426 DIELLPSEGDIVHDPYCWSAYGISQTLTIPPVSCLLEGLPQSIS-GNDIDHC 474

BLAST of CSPI07G18450 vs. TrEMBL
Match: A0A151SMX4_CAJCA (Polygalacturonase At1g48100 family OS=Cajanus cajan GN=KK1_002367 PE=3 SV=1)

HSP 1 Score: 724.2 bits (1868), Expect = 1.0e-205
Identity = 341/453 (75.28%), Postives = 386/453 (85.21%), Query Frame = 1

Query: 24  QARWHHHTKQKHSHRHRHSYISHPPSLSPSPSDSPDDG---------YYINDTNIFDVRA 83
           QAR H+HTK KHSH H    IS  P+   +PS +PD+           Y N +N FD+R 
Sbjct: 23  QARHHYHTKHKHSHYHNSPEISPSPAPFSAPSSAPDEAPSPSPSFNENYHNASNFFDLRT 82

Query: 84  FGAIGDGIVDDTEAFKMAWDTACQSEDDSVVMLVPYGFSFMIQSTIFTGPCHGYLMLRVD 143
           FGAIGDGI DDTE+FKMAWDTACQSE     ++VP G SF+IQSTIFTGPC G L+L+VD
Sbjct: 83  FGAIGDGITDDTESFKMAWDTACQSESSVKTIIVPRGLSFIIQSTIFTGPCKGGLVLKVD 142

Query: 144 GTLMTPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGRGQKWWDLPCKPHKGPNGTT 203
           GTL+ PDGP++WPKN SRRQWLVFYRI+GMSLEG GVIDGRG KWWDLPCKPHKGPNGTT
Sbjct: 143 GTLIPPDGPESWPKNNSRRQWLVFYRINGMSLEGSGVIDGRGAKWWDLPCKPHKGPNGTT 202

Query: 204 LPGPCDSPIAIRFFMSSNLTVHGLKITNSPQFHFRFDGCKTVHIDSIHIKSPGLSPNTDG 263
            PGPCDSP+AIRFFMSSNLTV GL+I NSPQFHFRFDGC++VH+DSI I +P LSPNTDG
Sbjct: 203 SPGPCDSPVAIRFFMSSNLTVQGLRIKNSPQFHFRFDGCESVHVDSIFITAPALSPNTDG 262

Query: 264 IHIENTNGVQIYNSVINNGDDCVSIGSGSYDLDIRNITCGPSHGISIGSLGVRNSRACVT 323
           IHIENTN V+IYNSVI+NGDDCVSIG+G +D+DI+N+TCGP HGISIGSLG  NSRACV+
Sbjct: 263 IHIENTNDVKIYNSVISNGDDCVSIGAGCHDVDIKNMTCGPGHGISIGSLGNHNSRACVS 322

Query: 324 NITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPIIIDQFYCLTKDCTNQ 383
           NITVRDSVIK SDNGVRIKTWQGG G+V  +TFSNIHM++VRNPIIIDQFYCLTKDC+N+
Sbjct: 323 NITVRDSVIKVSDNGVRIKTWQGGSGSVSRVTFSNIHMESVRNPIIIDQFYCLTKDCSNK 382

Query: 384 TSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIELLPAQGDIVLDPFCWN 443
           TSAV V+DI YTNIKGTYDIR PPMHFACSDSVPCTNLTLS+IELLPA GDI+LDPFCW+
Sbjct: 383 TSAVFVTDILYTNIKGTYDIRHPPMHFACSDSVPCTNLTLSDIELLPALGDIMLDPFCWS 442

Query: 444 AYGDLQTLTIPPVSCLQEGLPSSVNFNNDNEYC 468
           AYG+ QTLTIPP+SCLQEGLP SV   ND ++C
Sbjct: 443 AYGNSQTLTIPPISCLQEGLPQSVP-GNDIDHC 474

BLAST of CSPI07G18450 vs. TrEMBL
Match: I1JBC8_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_02G009300 PE=3 SV=2)

HSP 1 Score: 723.0 bits (1865), Expect = 2.3e-205
Identity = 343/472 (72.67%), Postives = 403/472 (85.38%), Query Frame = 1

Query: 6   LLSFAIWLSFVSCLLSSSQARWHHHTKQKHSHRHRHSYISHPPSLSPSPSDSPDDG---- 65
           +L+ A+ L F++  L + QAR H+H K KHS+ H    IS  PS +P+PS++PDD     
Sbjct: 6   ILTLALSLYFLALFLPT-QARHHYHKKHKHSYYHNAPEIS--PSPAPAPSNAPDDASSPS 65

Query: 66  ------YYINDTNIFDVRAFGAIGDGIVDDTEAFKMAWDTACQSEDDSVVMLVPYGFSFM 125
                 Y+   +N+FDVR FGAIGDGI DDTE+FKMAWDTAC+SE    V+LVP GFSF+
Sbjct: 66  PSGNENYHNASSNLFDVRTFGAIGDGITDDTESFKMAWDTACESESPVKVILVPQGFSFV 125

Query: 126 IQSTIFTGPCHGYLMLRVDGTLMTPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGR 185
           IQSTIFTGPC G L+L+VDGTLM PDGP++WPKN S+RQWLVFYRI+GMSLEG G+IDGR
Sbjct: 126 IQSTIFTGPCKGGLVLKVDGTLMPPDGPESWPKNNSKRQWLVFYRINGMSLEGSGLIDGR 185

Query: 186 GQKWWDLPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTVHGLKITNSPQFHFRFDGCKT 245
           G KWWDLPCKPHKGPNGTT PGPCDSP+AIRFFMSSNLTV GL+I NSPQFHFRFDGC++
Sbjct: 186 GAKWWDLPCKPHKGPNGTTSPGPCDSPVAIRFFMSSNLTVQGLRIKNSPQFHFRFDGCES 245

Query: 246 VHIDSIHIKSPGLSPNTDGIHIENTNGVQIYNSVINNGDDCVSIGSGSYDLDIRNITCGP 305
           VH++SI+I +P LSPNTDGIHIENTN V+IYNSVI+NGDDCVSIG+G +D+DI+NITCGP
Sbjct: 246 VHVESIYITAPALSPNTDGIHIENTNDVRIYNSVISNGDDCVSIGAGCHDVDIKNITCGP 305

Query: 306 SHGISIGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNV 365
            HGISIGSLG  NSRACV+NITVRDSVIK +DNGVRIKTWQGG G+V G+TFSNIHM++V
Sbjct: 306 GHGISIGSLGNHNSRACVSNITVRDSVIKVADNGVRIKTWQGGSGSVSGVTFSNIHMESV 365

Query: 366 RNPIIIDQFYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTLS 425
           RNPIIIDQFYCL+KDC+N+TSAV V+DI YTNIKGTYDIR PPM FACSDSVPCTNLTLS
Sbjct: 366 RNPIIIDQFYCLSKDCSNKTSAVFVTDIVYTNIKGTYDIRHPPMRFACSDSVPCTNLTLS 425

Query: 426 EIELLPAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSVNFNNDNEYC 468
           +IELLPAQG+IVLDPFCW+AYG+ +TLTIPPV C+ EGLP S++  ND ++C
Sbjct: 426 DIELLPAQGNIVLDPFCWSAYGNSETLTIPPVFCMLEGLPQSIS-GNDIDHC 473

BLAST of CSPI07G18450 vs. TAIR10
Match: AT1G02460.1 (AT1G02460.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 656.4 bits (1692), Expect = 1.3e-188
Identity = 311/472 (65.89%), Postives = 377/472 (79.87%), Query Frame = 1

Query: 12  WLSFVSCLLSSSQARWHHHTKQKHSHRHRHSYISHP----------------------PS 71
           +++ +  L+S S+AR HHH K+KH H + + + S P                      PS
Sbjct: 14  FMTLILTLMSLSEARNHHH-KEKHKHNNHNHHSSKPKPPSSSISQPPTPPPGPPDSPAPS 73

Query: 72  LSPSPSDSPDDGYYINDTNIFDVRAFGAIGDGIVDDTEAFKMAWDTACQSEDDS-VVMLV 131
           L PSPSD P D    ++  I++VR +GA+GDG  DDTEAFK AWD++C +E+++  V+LV
Sbjct: 74  LPPSPSDDPAD----DNNGIYNVRKYGAVGDGETDDTEAFKTAWDSSCNNENNTDSVLLV 133

Query: 132 PYGFSFMIQSTIFTGPCHGYLMLRVDGTLMTPDGPDAWPKNASRRQWLVFYRIDGMSLEG 191
           PYG++FMIQSTIFTGPC  Y   +VDGT++TPDGP++WP N S+RQWLVFYR++GM+L+G
Sbjct: 134 PYGYTFMIQSTIFTGPCRSYQFFQVDGTIVTPDGPESWPSNISKRQWLVFYRVNGMALKG 193

Query: 192 GGVIDGRGQKWWDLPCKPHKGPNGTTL-PGPCDSPIAIRFFMSSNLTVHGLKITNSPQFH 251
            GVIDGRGQKWWDLPCKPH+  N + +  GPCDSPIA+RFFMSSNL V GL+I NSPQFH
Sbjct: 194 EGVIDGRGQKWWDLPCKPHRSVNKSAIVTGPCDSPIALRFFMSSNLRVEGLQIKNSPQFH 253

Query: 252 FRFDGCKTVHIDSIHIKSPGLSPNTDGIHIENTNGVQIYNSVINNGDDCVSIGSGSYDLD 311
           FRFDGC+ VH++S+HI +P LSPNTDGIHIEN+N V IYNS+I+NGDDCVSIGSGSYD+D
Sbjct: 254 FRFDGCQGVHVESLHITAPPLSPNTDGIHIENSNSVTIYNSIISNGDDCVSIGSGSYDVD 313

Query: 312 IRNITCGPS-HGISIGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGIT 371
           IRN+TCGP  HGISIGSLG  NSRACV+NITVRDSVIK SDNGVRIKTWQGG G+V G+T
Sbjct: 314 IRNLTCGPGGHGISIGSLGNHNSRACVSNITVRDSVIKYSDNGVRIKTWQGGSGSVSGVT 373

Query: 372 FSNIHMDNVRNPIIIDQFYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDS 431
           F+NIH+D+VRNPIIIDQ+YC+TKDC N+TSAV VSDI Y  IKGTYDIRSPPMHF CSD+
Sbjct: 374 FNNIHVDSVRNPIIIDQYYCMTKDCANKTSAVFVSDIAYQGIKGTYDIRSPPMHFGCSDA 433

Query: 432 VPCTNLTLSEIELLPAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSV 459
           VPCTNLTLS+IELLPA+G+IVLDPFCWNAYG  + L+IPPV CL    P  +
Sbjct: 434 VPCTNLTLSDIELLPAKGEIVLDPFCWNAYGIAEELSIPPVWCLMSDPPKGL 480

BLAST of CSPI07G18450 vs. TAIR10
Match: AT4G01890.1 (AT4G01890.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 585.1 bits (1507), Expect = 3.8e-167
Identity = 276/449 (61.47%), Postives = 340/449 (75.72%), Query Frame = 1

Query: 17  SCLLSSSQARWHHHTKQKHSHRHRHSYISHPPSLSPSPSD---SPDDGYYINDTNIFDVR 76
           S L   ++AR HH   Q             PPS SP+P+D   SP     ++   ++DVR
Sbjct: 22  SLLSYGTEARLHHQASQ-------------PPSPSPNPNDPSKSPSRSQDLDHEVVYDVR 81

Query: 77  AFGAIGDGIVDDTEAFKMAWDTACQSEDDSV--VMLVPYGFSFMIQSTIFTGPCHGYLML 136
            +GA+G+G+ DDT +FK AWD+AC +  ++   V+ VPYGF+FMI+STIFTGPC  Y   
Sbjct: 82  KYGAVGNGVADDTVSFKTAWDSACSNNKNNTASVLHVPYGFTFMIRSTIFTGPCRSYQYF 141

Query: 137 RVDGTLMTPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGRGQKWWDLPCKPHK-GP 196
           +VDGT++  DGP +WP + ++RQWL FYRI+GM+L+G GVIDGRGQ WWDLPCKPH+   
Sbjct: 142 QVDGTIVPRDGPKSWPSSLNKRQWLAFYRINGMALQGAGVIDGRGQNWWDLPCKPHQQNV 201

Query: 197 NGTTLPGPCDSPIAIRFFMSSNLTVHGLKITNSPQFHFRFDGCKTVHIDSIHIKSPGLSP 256
           N T L GPC+SP A+RFFMSSN+ V GL I NSPQ H + DGC  VHI+S+ I SP  SP
Sbjct: 202 NKTKLAGPCESPAALRFFMSSNVIVKGLSIKNSPQVHLKLDGCHVVHINSLRIISPPASP 261

Query: 257 NTDGIHIENTNGVQIYNSVINNGDDCVSIGSGSYDLDIRNITCGPS-HGISIGSLGVRNS 316
           NTDGIHIEN+N V+IYNSVI+NGDDCVSIG G+YD+DIRNITCGP  HGISIGSLG +NS
Sbjct: 262 NTDGIHIENSNSVEIYNSVISNGDDCVSIGPGAYDIDIRNITCGPGGHGISIGSLGEKNS 321

Query: 317 RACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPIIIDQFYCLTK 376
            ACV+N+TVRDS IK S+NGVRIKTWQGG G+V G+TF NIH+D VRNPIIIDQ+YC TK
Sbjct: 322 HACVSNVTVRDSFIKFSENGVRIKTWQGGSGSVSGVTFDNIHVDTVRNPIIIDQYYCTTK 381

Query: 377 DCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIELLPAQGDIVLD 436
            C N+TSAV V+DI Y +IKGTYDIRSPPMHF CS++VPCTNLTLS IELLP++ DIV+ 
Sbjct: 382 SCANKTSAVFVNDIVYQSIKGTYDIRSPPMHFGCSNNVPCTNLTLSNIELLPSKEDIVVG 441

Query: 437 PFCWNAYGDLQTLTIPPVSCLQEGLPSSV 459
           PFCWNAYG     ++P +SCL+    +S+
Sbjct: 442 PFCWNAYGITDEFSVPLISCLKSNPSTSL 457

BLAST of CSPI07G18450 vs. TAIR10
Match: AT1G48100.1 (AT1G48100.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 560.1 bits (1442), Expect = 1.3e-159
Identity = 266/474 (56.12%), Postives = 343/474 (72.36%), Query Frame = 1

Query: 3   SFVLLSFAIWLSFVSCLLSSSQARWHHHTKQKHSHRHRHSYISHPPSLSPSPSD------ 62
           S +L+ + I         + ++AR+H+H K +H   H       PP  + +P D      
Sbjct: 5   SGILVIYIISTVLFGHFTNVARARYHYH-KGRHGVTHPLPPPPPPPLETANPPDQVPSDP 64

Query: 63  --SPDDGYYINDTN-IFDVRAFGAIGDGIVDDTEAFKMAWDTACQSEDDSVVMLVPYGFS 122
             SPD     +D+  +FDV +FGA+GDG  DDT AF+ AW  AC  E  S V+L P G  
Sbjct: 65  YPSPDPAPGDSDSGCVFDVTSFGAVGDGSCDDTAAFQDAWKAACAVE--SGVVLAPEGGV 124

Query: 123 FMIQSTIFTGPCHGYLMLRVDGTLMTPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVID 182
           F I STIF+GPC   L+ ++DG LM PDGP+ WP+  ++ QWLVFYR+DG +  G G ++
Sbjct: 125 FKITSTIFSGPCKPGLVFQLDGVLMPPDGPEEWPEKDNKNQWLVFYRLDGFTFSGKGTVE 184

Query: 183 GRGQKWWDLPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTVHGLKITNSPQFHFRFDGC 242
           G GQKWWDLPCKPH+GP+G++  GPC SP  IRFFMS+N+ V GL+I NSPQFH +FDGC
Sbjct: 185 GNGQKWWDLPCKPHRGPDGSSSSGPCASPTMIRFFMSNNIEVKGLRIQNSPQFHMKFDGC 244

Query: 243 KTVHIDSIHIKSPGLSPNTDGIHIENTNGVQIYNSVINNGDDCVSIGSGSYDLDIRNITC 302
           + V I+ I I SP LSPNTDGIH+ NT  V IYNSV++NGDDC+SIG+G  D+DI+ +TC
Sbjct: 245 QGVLINEIQISSPKLSPNTDGIHLGNTRSVGIYNSVVSNGDDCISIGTGCSDVDIQGVTC 304

Query: 303 GPSHGISIGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMD 362
           GPSHGISIGSLGV NS+ACV+NITVR++VI+DSDNG+R+KTWQGG G+V  + F NI M+
Sbjct: 305 GPSHGISIGSLGVHNSQACVSNITVRNTVIRDSDNGLRVKTWQGGTGSVSNLLFENIQME 364

Query: 363 NVRNPIIIDQFYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLT 422
           NV N II+DQ+YC +KDC N+TSAV V D++Y NIKGTYD+RSPP+HFACSD+V CTN+T
Sbjct: 365 NVLNCIIVDQYYCQSKDCRNETSAVKVFDVEYRNIKGTYDVRSPPIHFACSDTVACTNIT 424

Query: 423 LSEIELLPAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSVNFNNDNEYC 468
           +SE+ELLP +G++V DPFCWNAYG  +TLTIPP+ CL +G P      + N  C
Sbjct: 425 MSEVELLPEEGELVDDPFCWNAYGKQETLTIPPIDCLLDGSPVVEEAYDSNYGC 475

BLAST of CSPI07G18450 vs. TAIR10
Match: AT1G56710.1 (AT1G56710.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 556.2 bits (1432), Expect = 1.9e-158
Identity = 254/411 (61.80%), Postives = 322/411 (78.35%), Query Frame = 1

Query: 49  SLSPSPSDSPDDGYYINDT---NIFDVRAFGAIGDGIVDDTEAFKMAWDTACQSEDD-SV 108
           S+  SPS +P+  Y  ND     +FDV +FGAIGD   DDT AFKMAWD AC S    S 
Sbjct: 24  SIYLSPSPAPNPAYNDNDNIAPTVFDVTSFGAIGDCSTDDTSAFKMAWDAACMSTGPKSA 83

Query: 109 VMLVPYGFSFMIQSTIFTGPCHGYLMLRVDGTLMTPDGPDAWPKNASRRQWLVFYRIDGM 168
           ++LVPY F F+++ T F GPC   L+L++DG +++PDGP +WP N  +RQW++FYR++G+
Sbjct: 84  LLLVPYTFCFLVKPTTFNGPCRTNLVLQIDGFIVSPDGPRSWPSNY-QRQWMMFYRVNGL 143

Query: 169 SLEGGGVIDGRGQKWWDLPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTVHGLKITNSP 228
           S++G GVI+GRGQKWW+LPCKPHKG NGTT  GPCDSP+AIR F SS + + G+   NS 
Sbjct: 144 SIQGSGVINGRGQKWWNLPCKPHKGLNGTTQTGPCDSPVAIRLFQSSKVRIQGINFMNSA 203

Query: 229 QFHFRFDGCKTVHIDSIHIKSPGLSPNTDGIHIENTNGVQIYNSVINNGDDCVSIGSGSY 288
           QFH RFD C  V +DS+ IK+P  SPNTDGIHIENT+ VQI NS+I+NGDDC+SIG+G +
Sbjct: 204 QFHVRFDNCSDVVVDSVIIKAPASSPNTDGIHIENTHNVQIRNSMISNGDDCISIGAGCF 263

Query: 289 DLDIRNITCGPSHGISIGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRG 348
           ++DI+N+TCGPSHGISIGSLGV NS+A V+NITV +S I +SDNGVRIKTWQGG G+V  
Sbjct: 264 NVDIKNVTCGPSHGISIGSLGVHNSQAYVSNITVTNSTIWNSDNGVRIKTWQGGSGSVSR 323

Query: 349 ITFSNIHMDNVRNPIIIDQFYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACS 408
           I FSNI M NVRNPI+IDQ+YC T +C NQTSAV +SD+ Y NIKGTYD+RSPP+HF CS
Sbjct: 324 IVFSNILMVNVRNPIMIDQYYCQTNNCANQTSAVIISDVLYANIKGTYDLRSPPIHFGCS 383

Query: 409 DSVPCTNLTLSEIELLPAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLP 456
           DSVPCTNLTL+E++L P++G  + +PFCWNAYG ++ +T+PPV CL +  P
Sbjct: 384 DSVPCTNLTLTEVDLFPSKGQHLENPFCWNAYGSMK-ITVPPVYCLLDAPP 432

BLAST of CSPI07G18450 vs. TAIR10
Match: AT1G60590.1 (AT1G60590.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 377.9 bits (969), Expect = 9.3e-105
Identity = 198/538 (36.80%), Postives = 299/538 (55.58%), Query Frame = 1

Query: 1   MSSFVLLSFAIWLSFVSCLLSSSQARWHHH------------TKQKHSHRHRHSYISH-- 60
           ++   L++  +W + +   ++     W HH            +K+  SH + H ++SH  
Sbjct: 12  ITMMTLVTILVWSATLETCIARRGRHWRHHHRSSSSLSDSLSSKKPKSHENNHHHMSHKS 71

Query: 61  ------------------------------PPSLSPSPSDSPDDGYYINDTNIFDVRAFG 120
                                         PP + P P+  P     + D+  F+V  FG
Sbjct: 72  KPKPKMKTQPPKSNDGSPVVSQPPQVQQPPPPHVQPPPTPLPLQP--VEDSQQFNVLDFG 131

Query: 121 AIGDGIVDDTEAFKMAWDTACQSEDDSVVMLVPYGFSFMIQSTIFTGP-CHGYLMLRVDG 180
           A GDG+ DDT+AF+ AW +AC+ E  +  M++P  + F++    F+GP C   ++ +++G
Sbjct: 132 AKGDGMSDDTQAFEAAWASACKVE--ASTMIIPPDYIFLVGPISFSGPYCQANIVFQLEG 191

Query: 181 TLMTPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGRGQKWW--------------- 240
            ++ P   ++W        W+ F ++ G++++G GVIDGRG  WW               
Sbjct: 192 MIVAPTDTESW--GGGLMWWIEFTKLSGITIQGNGVIDGRGTVWWQQDYLSDYPIDDDFK 251

Query: 241 ----------DLPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTVHGLKITNSPQFHFRF 300
                     + P  P +      +P     P A+RF+ S ++TV G+ I NSPQ H +F
Sbjct: 252 LIVPLNNSVQERPPMPIRSELNWRMPS--IKPTALRFYGSIDVTVTGITIQNSPQCHLKF 311

Query: 301 DGCKTVHIDSIHIKSPGLSPNTDGIHIENTNGVQIYNSVINNGDDCVSIGSGSYDLDIRN 360
           D C  V +  +++ SPG SPNTDGIH++NT  V I+ + +  GDDC+SI +G  ++ + N
Sbjct: 312 DNCVKVLVHDVNVSSPGDSPNTDGIHLQNTRDVMIHTTTLACGDDCISIQTGCSNVYVHN 371

Query: 361 ITCGPSHGISIGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNI 420
           + CGP HGISIGSLG  +++ACV+NITVRD V+ ++  GVRIKTWQGG G+V+GI FSNI
Sbjct: 372 VNCGPGHGISIGSLGKDSTKACVSNITVRDVVMHNTMTGVRIKTWQGGIGSVKGILFSNI 431

Query: 421 HMDNVRNPIIIDQFYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCT 468
            +  V+ PI+IDQFYC    C N TSAVSV  + Y  I+GTY ++  P+HFACSDS PC 
Sbjct: 432 QLTEVQLPIVIDQFYCDHSKCMNHTSAVSVEGVTYEKIRGTYTVK--PVHFACSDSFPCI 491

BLAST of CSPI07G18450 vs. NCBI nr
Match: gi|449436389|ref|XP_004135975.1| (PREDICTED: polygalacturonase At1g48100 [Cucumis sativus])

HSP 1 Score: 987.3 bits (2551), Expect = 9.5e-285
Identity = 467/467 (100.00%), Postives = 467/467 (100.00%), Query Frame = 1

Query: 1   MSSFVLLSFAIWLSFVSCLLSSSQARWHHHTKQKHSHRHRHSYISHPPSLSPSPSDSPDD 60
           MSSFVLLSFAIWLSFVSCLLSSSQARWHHHTKQKHSHRHRHSYISHPPSLSPSPSDSPDD
Sbjct: 1   MSSFVLLSFAIWLSFVSCLLSSSQARWHHHTKQKHSHRHRHSYISHPPSLSPSPSDSPDD 60

Query: 61  GYYINDTNIFDVRAFGAIGDGIVDDTEAFKMAWDTACQSEDDSVVMLVPYGFSFMIQSTI 120
           GYYINDTNIFDVRAFGAIGDGIVDDTEAFKMAWDTACQSEDDSVVMLVPYGFSFMIQSTI
Sbjct: 61  GYYINDTNIFDVRAFGAIGDGIVDDTEAFKMAWDTACQSEDDSVVMLVPYGFSFMIQSTI 120

Query: 121 FTGPCHGYLMLRVDGTLMTPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGRGQKWW 180
           FTGPCHGYLMLRVDGTLMTPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGRGQKWW
Sbjct: 121 FTGPCHGYLMLRVDGTLMTPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGRGQKWW 180

Query: 181 DLPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTVHGLKITNSPQFHFRFDGCKTVHIDS 240
           DLPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTVHGLKITNSPQFHFRFDGCKTVHIDS
Sbjct: 181 DLPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTVHGLKITNSPQFHFRFDGCKTVHIDS 240

Query: 241 IHIKSPGLSPNTDGIHIENTNGVQIYNSVINNGDDCVSIGSGSYDLDIRNITCGPSHGIS 300
           IHIKSPGLSPNTDGIHIENTNGVQIYNSVINNGDDCVSIGSGSYDLDIRNITCGPSHGIS
Sbjct: 241 IHIKSPGLSPNTDGIHIENTNGVQIYNSVINNGDDCVSIGSGSYDLDIRNITCGPSHGIS 300

Query: 301 IGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPII 360
           IGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPII
Sbjct: 301 IGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPII 360

Query: 361 IDQFYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIELL 420
           IDQFYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIELL
Sbjct: 361 IDQFYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIELL 420

Query: 421 PAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSVNFNNDNEYC 468
           PAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSVNFNNDNEYC
Sbjct: 421 PAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSVNFNNDNEYC 467

BLAST of CSPI07G18450 vs. NCBI nr
Match: gi|659122922|ref|XP_008461394.1| (PREDICTED: polygalacturonase At1g48100-like [Cucumis melo])

HSP 1 Score: 973.4 bits (2515), Expect = 1.4e-280
Identity = 459/467 (98.29%), Postives = 462/467 (98.93%), Query Frame = 1

Query: 1   MSSFVLLSFAIWLSFVSCLLSSSQARWHHHTKQKHSHRHRHSYISHPPSLSPSPSDSPDD 60
           MSSFVLLSFAIWLSFVSCLLSSSQARWHHHTKQKHSH HRH YISHPPSLSPSPSDSPDD
Sbjct: 1   MSSFVLLSFAIWLSFVSCLLSSSQARWHHHTKQKHSHHHRHGYISHPPSLSPSPSDSPDD 60

Query: 61  GYYINDTNIFDVRAFGAIGDGIVDDTEAFKMAWDTACQSEDDSVVMLVPYGFSFMIQSTI 120
           G YINDTNIFDVRAFGAIGDGIVDDTEAFKMAWDTACQSEDDS VMLVPYGFSFMIQSTI
Sbjct: 61  GDYINDTNIFDVRAFGAIGDGIVDDTEAFKMAWDTACQSEDDSAVMLVPYGFSFMIQSTI 120

Query: 121 FTGPCHGYLMLRVDGTLMTPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGRGQKWW 180
           FTGPCHGYLMLRVDGTLM PDGPDAWPKN+SRRQWLVFYRIDGMSLEGGGVIDGRGQKWW
Sbjct: 121 FTGPCHGYLMLRVDGTLMPPDGPDAWPKNSSRRQWLVFYRIDGMSLEGGGVIDGRGQKWW 180

Query: 181 DLPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTVHGLKITNSPQFHFRFDGCKTVHIDS 240
           DLPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTVHGLKITNSPQFHFRFDGCKTVHIDS
Sbjct: 181 DLPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTVHGLKITNSPQFHFRFDGCKTVHIDS 240

Query: 241 IHIKSPGLSPNTDGIHIENTNGVQIYNSVINNGDDCVSIGSGSYDLDIRNITCGPSHGIS 300
           IHIKSPGLSPNTDGIHIENTNGV+IYNSVINNGDDCVSIGSGSYDLDIRNITCGPSHGIS
Sbjct: 241 IHIKSPGLSPNTDGIHIENTNGVEIYNSVINNGDDCVSIGSGSYDLDIRNITCGPSHGIS 300

Query: 301 IGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPII 360
           IGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPII
Sbjct: 301 IGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPII 360

Query: 361 IDQFYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIELL 420
           IDQFYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIELL
Sbjct: 361 IDQFYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIELL 420

Query: 421 PAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSVNFNNDNEYC 468
           PAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSS+NFNNDNEYC
Sbjct: 421 PAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSINFNNDNEYC 467

BLAST of CSPI07G18450 vs. NCBI nr
Match: gi|743791168|ref|XP_011040652.1| (PREDICTED: polygalacturonase At1g48100-like [Populus euphratica])

HSP 1 Score: 750.4 bits (1936), Expect = 1.9e-213
Identity = 362/468 (77.35%), Postives = 400/468 (85.47%), Query Frame = 1

Query: 6   LLSFAIWLSFVSCLLSSSQARWHHHTKQ-KHSHRHRHSYISHPPSLSPSPSDSPD----- 65
           LL F I  SF +CL    QARWH+HTK  KHSH H+ S IS PP+  P P+  P      
Sbjct: 8   LLVFGI--SF-TCLFLGIQARWHYHTKHHKHSHLHKSSTISEPPAPPPEPASHPSVPANP 67

Query: 66  DGYYINDTNIFDVRAFGAIGDGIVDDTEAFKMAWDTACQSEDDSVVMLVPYGFSFMIQST 125
            G   N T +FDVR+FGAIGDG+ DDT+AFKMAWD AC ++DDS V+LVPYGF FMIQST
Sbjct: 68  SGGSGNSTGVFDVRSFGAIGDGVTDDTDAFKMAWDAAC-NQDDSPVILVPYGFQFMIQST 127

Query: 126 IFTGPCHGYLMLRVDGTLMTPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGRGQKW 185
           IFTGPC G L+ +VDGTLM PDGPD+WP N SRRQWLVFYRI+ MSL GGGVIDGRG+KW
Sbjct: 128 IFTGPCQGGLVFQVDGTLMPPDGPDSWPHNNSRRQWLVFYRINEMSLLGGGVIDGRGEKW 187

Query: 186 WDLPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTVHGLKITNSPQFHFRFDGCKTVHID 245
           WDLPCKPHKG NGTT+PGPCDSPIAIRFFMSSNLTV GLKI NSPQF+FRFD CK VH++
Sbjct: 188 WDLPCKPHKGINGTTMPGPCDSPIAIRFFMSSNLTVQGLKIKNSPQFNFRFDNCKNVHVE 247

Query: 246 SIHIKSPGLSPNTDGIHIENTNGVQIYNSVINNGDDCVSIGSGSYDLDIRNITCGPSHGI 305
           SIHI +P LSPNTDGIHIENTNGV+IYNSVI+NGDDCVSIGSG YD+DIRNITCGPSHGI
Sbjct: 248 SIHITAPALSPNTDGIHIENTNGVEIYNSVISNGDDCVSIGSGCYDVDIRNITCGPSHGI 307

Query: 306 SIGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPI 365
           SIGSLG  NSRACV+NITVRDSVI+ SDNGVRIKTWQGG GAV GITFSNIHMDNVRNPI
Sbjct: 308 SIGSLGNHNSRACVSNITVRDSVIRVSDNGVRIKTWQGGSGAVSGITFSNIHMDNVRNPI 367

Query: 366 IIDQFYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIEL 425
           IIDQFYCL+K CTNQTSA+SVSDI Y NIKGTY+IRSPPMHFACSDSVPCTNLTLS+IEL
Sbjct: 368 IIDQFYCLSKGCTNQTSALSVSDILYENIKGTYNIRSPPMHFACSDSVPCTNLTLSDIEL 427

Query: 426 LPAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSVNFNNDNEYC 468
           LPA+GD+VLDP+CWNAYG+ +TLTIPPVSCL EG+P S N NN  +YC
Sbjct: 428 LPAEGDLVLDPYCWNAYGNFRTLTIPPVSCLMEGIPRS-NLNNKMDYC 470

BLAST of CSPI07G18450 vs. NCBI nr
Match: gi|590720054|ref|XP_007051225.1| (Pectin lyase-like superfamily protein [Theobroma cacao])

HSP 1 Score: 740.7 bits (1911), Expect = 1.5e-210
Identity = 352/468 (75.21%), Postives = 395/468 (84.40%), Query Frame = 1

Query: 9   FAIWLSFVSCLLSSSQARWH-HHTKQKHSHRHRHSYISHPP--------SLSPSPSDSPD 68
           F   +SFV C   S+QARWH HHTK KH+H H  S IS PP         L+P PS   D
Sbjct: 9   FIFCMSFV-CFFLSTQARWHYHHTKHKHTHHHNISEISAPPITAPEPSSPLAPEPSSPSD 68

Query: 69  DGYYINDTNIFDVRAFGAIGDGIVDDTEAFKMAWDTACQSEDDSVVMLVPYGFSFMIQST 128
           DG   N T +FDVR FGA+GDG+ DDTEAFKMAWD+AC+   +S V+ VPYGFSFMIQST
Sbjct: 69  DG---NATGVFDVRKFGAVGDGVTDDTEAFKMAWDSACRV--NSAVLHVPYGFSFMIQST 128

Query: 129 IFTGPCHGYLMLRVDGTLMTPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGRGQKW 188
           IF GPC G L+ +VDGTLM PDGPDAWP+N S+RQWLVFYRI+GMSL+GGG+IDGRGQKW
Sbjct: 129 IFLGPCQGGLVFQVDGTLMPPDGPDAWPENTSKRQWLVFYRINGMSLQGGGLIDGRGQKW 188

Query: 189 WDLPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTVHGLKITNSPQFHFRFDGCKTVHID 248
           WDLPCKPHKG N T LPGPCDSPIA+RFFMSSNLTV GLKI +SP+FHFRFDGC+ VH++
Sbjct: 189 WDLPCKPHKGKNRTALPGPCDSPIALRFFMSSNLTVKGLKIKDSPKFHFRFDGCQNVHVE 248

Query: 249 SIHIKSPGLSPNTDGIHIENTNGVQIYNSVINNGDDCVSIGSGSYDLDIRNITCGPSHGI 308
           S+HI +P LSPNTDGIHIENTNGV+IYNSVI+NGDDC+SIGSG +D+DI+N+TCGP HGI
Sbjct: 249 SLHITAPALSPNTDGIHIENTNGVEIYNSVISNGDDCISIGSGCHDVDIKNLTCGPGHGI 308

Query: 309 SIGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPI 368
           SIGSLG  NSRACV+N+TVRDSVIK SDNGVRIKTWQGG GAV GITFSNIHM+ VRNPI
Sbjct: 309 SIGSLGNHNSRACVSNVTVRDSVIKVSDNGVRIKTWQGGSGAVSGITFSNIHMEAVRNPI 368

Query: 369 IIDQFYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIEL 428
           IIDQFYCLTK CTNQTSAV VSDI Y +IKGTYDIRSPPMHFACSDSVPCTN+TLS+IEL
Sbjct: 369 IIDQFYCLTKGCTNQTSAVYVSDILYESIKGTYDIRSPPMHFACSDSVPCTNITLSDIEL 428

Query: 429 LPAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSVNFNNDNEYC 468
           LPAQGDIVLDPFCWNAYGDL++LTIPPVSCL  G+P S+  N D  YC
Sbjct: 429 LPAQGDIVLDPFCWNAYGDLESLTIPPVSCLMAGIPRSILDNKDMGYC 470

BLAST of CSPI07G18450 vs. NCBI nr
Match: gi|593688996|ref|XP_007145117.1| (hypothetical protein PHAVU_007G211400g [Phaseolus vulgaris])

HSP 1 Score: 728.4 bits (1879), Expect = 7.9e-207
Identity = 346/472 (73.31%), Postives = 401/472 (84.96%), Query Frame = 1

Query: 6   LLSFAIWLSFVSCLLSSSQARWHHHTKQKHSHRHRHSYISHPPSLSPSPSDSPDDG---- 65
           +LS A+ L F++  L + QAR+H H K KHSH H    IS  PS  P PS +PD+     
Sbjct: 6   ILSLALSLYFLTLFLPT-QARYHFH-KNKHSHYHNAPEISPSPSPLPGPSSAPDEAPSPS 65

Query: 66  ------YYINDTNIFDVRAFGAIGDGIVDDTEAFKMAWDTACQSEDDSVVMLVPYGFSFM 125
                 Y+    N+FD+RAFGAIGDG+ DDTE+FKMAWDTACQSE    V+LVP+G SF+
Sbjct: 66  PTVDENYHNTSNNLFDIRAFGAIGDGVTDDTESFKMAWDTACQSESAVKVILVPHGLSFI 125

Query: 126 IQSTIFTGPCHGYLMLRVDGTLMTPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGR 185
           IQSTIFTGPC G L+L+VDGTLM PDGPD+WPKN SRRQWLVFYRI+GMSLEG G+IDGR
Sbjct: 126 IQSTIFTGPCKGGLVLKVDGTLMPPDGPDSWPKNNSRRQWLVFYRINGMSLEGSGLIDGR 185

Query: 186 GQKWWDLPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTVHGLKITNSPQFHFRFDGCKT 245
           G+KWWDLPCKPHKGP+GTT PGPCDSP+A+RFFMSSNLTV GLKI NSPQFHFRFDGC++
Sbjct: 186 GEKWWDLPCKPHKGPHGTTSPGPCDSPVALRFFMSSNLTVQGLKIKNSPQFHFRFDGCES 245

Query: 246 VHIDSIHIKSPGLSPNTDGIHIENTNGVQIYNSVINNGDDCVSIGSGSYDLDIRNITCGP 305
           VH++SI+I SP LSPNTDGIHIENTN V+IYNSVI+NGDDCVSIG+G +D+DI+NITCGP
Sbjct: 246 VHVESIYITSPALSPNTDGIHIENTNDVKIYNSVISNGDDCVSIGAGCHDVDIKNITCGP 305

Query: 306 SHGISIGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNV 365
            HGISIGSLG  NSRACV+NITVRDSVIK SDNGVRIKTWQGG G+V G+TF+NIHM++V
Sbjct: 306 GHGISIGSLGNHNSRACVSNITVRDSVIKVSDNGVRIKTWQGGSGSVSGVTFTNIHMESV 365

Query: 366 RNPIIIDQFYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTLS 425
           RNPIIIDQFYCL+KDC+N+TSAV VS+I YTNIKGTYDIR PPMHFACSDSVPCTNLTLS
Sbjct: 366 RNPIIIDQFYCLSKDCSNKTSAVFVSNISYTNIKGTYDIRHPPMHFACSDSVPCTNLTLS 425

Query: 426 EIELLPAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSVNFNNDNEYC 468
           +IELLP++GDIV DP+CW+AYG  QTLTIPPVSCL EGLP S++  ND ++C
Sbjct: 426 DIELLPSEGDIVHDPYCWSAYGISQTLTIPPVSCLLEGLPQSIS-GNDIDHC 474

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
PGLR4_ARATH2.3e-15856.12Polygalacturonase At1g48100 OS=Arabidopsis thaliana GN=At1g48100 PE=2 SV=1[more]
PGLR5_ARATH2.2e-9243.52Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana GN=At1g80170 PE=1 S... [more]
PGLR2_CRYJA2.5e-8344.17Polygalacturonase OS=Cryptomeria japonica PE=1 SV=1[more]
PGLR2_CHAOB1.8e-8144.26Polygalacturonase OS=Chamaecyparis obtusa PE=1 SV=1[more]
PGLR2_JUNAS7.4e-8042.90Polygalacturonase OS=Juniperus ashei GN=JNA2 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KBA7_CUCSA6.6e-285100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_7G414430 PE=3 SV=1[more]
A0A061DSI7_THECC1.1e-21075.21Pectin lyase-like superfamily protein OS=Theobroma cacao GN=TCM_004895 PE=3 SV=1[more]
V7BKM1_PHAVU5.5e-20773.31Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_007G211400g PE=3 SV=1[more]
A0A151SMX4_CAJCA1.0e-20575.28Polygalacturonase At1g48100 family OS=Cajanus cajan GN=KK1_002367 PE=3 SV=1[more]
I1JBC8_SOYBN2.3e-20572.67Uncharacterized protein OS=Glycine max GN=GLYMA_02G009300 PE=3 SV=2[more]
Match NameE-valueIdentityDescription
AT1G02460.11.3e-18865.89 Pectin lyase-like superfamily protein[more]
AT4G01890.13.8e-16761.47 Pectin lyase-like superfamily protein[more]
AT1G48100.11.3e-15956.12 Pectin lyase-like superfamily protein[more]
AT1G56710.11.9e-15861.80 Pectin lyase-like superfamily protein[more]
AT1G60590.19.3e-10536.80 Pectin lyase-like superfamily protein[more]
Match NameE-valueIdentityDescription
gi|449436389|ref|XP_004135975.1|9.5e-285100.00PREDICTED: polygalacturonase At1g48100 [Cucumis sativus][more]
gi|659122922|ref|XP_008461394.1|1.4e-28098.29PREDICTED: polygalacturonase At1g48100-like [Cucumis melo][more]
gi|743791168|ref|XP_011040652.1|1.9e-21377.35PREDICTED: polygalacturonase At1g48100-like [Populus euphratica][more]
gi|590720054|ref|XP_007051225.1|1.5e-21075.21Pectin lyase-like superfamily protein [Theobroma cacao][more]
gi|593688996|ref|XP_007145117.1|7.9e-20773.31hypothetical protein PHAVU_007G211400g [Phaseolus vulgaris][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000743Glyco_hydro_28
IPR006626PbH1
IPR011050Pectin_lyase_fold/virulence
IPR012334Pectin_lyas_fold
Vocabulary: Molecular Function
TermDefinition
GO:0004650polygalacturonase activity
Vocabulary: Biological Process
TermDefinition
GO:0005975carbohydrate metabolic process
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0071555 cell wall organization
biological_process GO:0090305 nucleic acid phosphodiester bond hydrolysis
biological_process GO:0005982 starch metabolic process
biological_process GO:0005985 sucrose metabolic process
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0005576 extracellular region
cellular_component GO:0005575 cellular_component
molecular_function GO:0008408 3'-5' exonuclease activity
molecular_function GO:0047911 galacturan 1,4-alpha-galacturonidase activity
molecular_function GO:0016829 lyase activity
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004650 polygalacturonase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI07G18450.1CSPI07G18450.1mRNA


Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000743Glycoside hydrolase, family 28PFAMPF00295Glyco_hydro_28coord: 104..419
score: 9.3
IPR000743Glycoside hydrolase, family 28PROSITEPS00502POLYGALACTURONASEcoord: 290..303
scor
IPR006626Parallel beta-helix repeatSMARTSM00710pbh1coord: 313..334
score: 1.1coord: 260..281
score: 260.0coord: 233..259
score: 650.0coord: 342..363
score: 7
IPR011050Pectin lyase fold/virulence factorunknownSSF51126Pectin lyase-likecoord: 67..460
score: 1.31E
IPR012334Pectin lyase foldGENE3DG3DSA:2.160.20.10coord: 54..456
score: 5.6E
NoneNo IPR availablePANTHERPTHR31375FAMILY NOT NAMEDcoord: 1..467
score:
NoneNo IPR availablePANTHERPTHR31375:SF43GLYCOSIDE HYDROLASE FAMILY 28 PROTEIN / POLYGALACTURONASE (PECTINASE) FAMILY PROTEINcoord: 1..467
score:

The following gene(s) are paralogous to this gene:

None