CSPI07G15690 (gene) Wild cucumber (PI 183967)

BLAST of CSPI07G15690 vs. Swiss-Prot
Match: HY6H_HYONI (Hyoscyamine 6-dioxygenase OS=Hyoscyamus niger GN=H6H PE=1 SV=1)

HSP 1 Score: 268.5 bits (685), Expect = 1.0e-70
Identity = 142/329 (43.16%), Postives = 203/329 (61.70%), Query Frame = 1

Query: 6   AKFVSNWETVESVPESYVYPPEKRPGNIVVPMAKAIPVIDLSIRDRTLLVRKILDASKEF 65
           A FVSNW T +SV ES++ P +KR    V P+   +P+IDL  +   LLV++I  A ++F
Sbjct: 2   ATFVSNWST-KSVSESFIAPLQKRAEKDV-PVGNDVPIIDLQ-QHHHLLVQQITKACQDF 61

Query: 66  GFFQIINHGVSKKVSEETMRIFKEFHAMSGPEKAKECSKDP--------NKSCRVYTSSE 125
           G FQ+INHG  +++  ETM + KEF A+   EK K   K           +  ++Y   E
Sbjct: 62  GLFQVINHGFPEELMLETMEVCKEFFALPAEEKEKFKPKGEAAKFELPLEQKAKLYVEGE 121

Query: 126 NYTKEQIHCWRDALIFNCHPLEK-YVHFWPQKPPKYREVVGAYCMAMRKLVLEILELMSE 185
             + E+   W+D L   CHPL++  V+ WP+KP KYREVV  Y + +RKL + +L+ + E
Sbjct: 122 QLSNEEFLYWKDTLAHGCHPLDQDLVNSWPEKPAKYREVVAKYSVEVRKLTMRMLDYICE 181

Query: 186 GLGLGKGYFGGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQ--VL 245
           GLGL  GYF  E+S+  ++L N+YPPCP+PS TLG   H D +LIT+L QD+ GLQ  ++
Sbjct: 182 GLGLKLGYFDNELSQIQMMLTNYYPPCPDPSSTLGSGGHYDGNLITLLQQDLPGLQQLIV 241

Query: 246 KDGQWIGVQPIDNAFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAM 305
           KD  WI VQPI  AFVVN+G  L++ITN K + + HR VT+    R S+  L+ P     
Sbjct: 242 KDATWIAVQPIPTAFVVNLGLTLKVITNEKFEGSIHRVVTDPTRDRVSIATLIGPDYSCT 301

Query: 306 MEPAKCMINEANPPRYRSLNFKDFQRNYL 324
           +EPAK ++N+ NPP Y+  ++ +F   YL
Sbjct: 302 IEPAKELLNQDNPPLYKPYSYSEFADIYL 327

BLAST of CSPI07G15690 vs. Swiss-Prot
Match: DMR6_ARATH (Protein DOWNY MILDEW RESISTANCE 6 OS=Arabidopsis thaliana GN=DMR6 PE=1 SV=1)

HSP 1 Score: 261.2 bits (666), Expect = 1.6e-68
Identity = 139/340 (40.88%), Postives = 205/340 (60.29%), Query Frame = 1

Query: 6   AKFVSNWETVESVPESYVYPPEKRPGNIVVPMAKAIPVIDLSIRDRTLLVRKILDASKEF 65
           AK +S      ++PE+YV P   RP    V   +  P+IDLS  DR+ L+++I  A   F
Sbjct: 3   AKLISTGFRHTTLPENYVRPISDRPRLSEVSQLEDFPLIDLSSTDRSFLIQQIHQACARF 62

Query: 66  GFFQIINHGVSKKVSEETMRIFKEFHAMSGPEKAKECSKDPNKSCRVYTSSENYTKEQIH 125
           GFFQ+INHGV+K++ +E + + +EF +MS  EK K  S DP K+ R+ TS  N  KE+++
Sbjct: 63  GFFQVINHGVNKQIIDEMVSVAREFFSMSMEEKMKLYSDDPTKTTRLSTSF-NVKKEEVN 122

Query: 126 CWRDALIFNCHPLEKYVHFWPQKPPKYREVVGAYCMAMRKLVLEILELMSEGLGLGKGYF 185
            WRD L  +C+P+ KYV+ WP  PP ++E+V  Y   +R++  +I EL+SE LGL K Y 
Sbjct: 123 NWRDYLRLHCYPIHKYVNEWPSNPPSFKEIVSKYSREVREVGFKIEELISESLGLEKDYM 182

Query: 186 GGEMSE-NPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQD--VNGLQVLKDGQWIGV 245
              + E    + VN+YPPCP P LT GL  H DP+ +TIL QD  V GLQ+L DGQW  V
Sbjct: 183 KKVLGEQGQHMAVNYYPPCPEPELTYGLPAHTDPNALTILLQDTTVCGLQILIDGQWFAV 242

Query: 246 QPIDNAFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAMMEPAKCM- 305
            P  +AFV+NIG  LQ ++NG  ++  HRAVTN++  R S+   + P D A+M PAK + 
Sbjct: 243 NPHPDAFVINIGDQLQALSNGVYKSVWHRAVTNTENPRLSVASFLCPADCAVMSPAKPLW 302

Query: 306 --INEANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIETN 340
              ++   P Y+   + ++ + +  R +D +  ++    N
Sbjct: 303 EAEDDETKPVYKDFTYAEYYKKFWSRNLDQEHCLENFLNN 341

BLAST of CSPI07G15690 vs. Swiss-Prot
Match: IDS3_HORVU (2'-deoxymugineic-acid 2'-dioxygenase OS=Hordeum vulgare GN=IDS3 PE=1 SV=3)

HSP 1 Score: 229.2 bits (583), Expect = 6.9e-59
Identity = 127/312 (40.71%), Postives = 188/312 (60.26%), Query Frame = 1

Query: 17  SVPESYVYPPEKRPGNIVVPMAKAI---PVIDLSIRDRTLLVRKILDASKEFGFFQIINH 76
           S+PES+V+  +K P     P  KA+   P+IDLS   R  + R IL+A KE GFFQ++NH
Sbjct: 13  SLPESFVFASDKVP-----PATKAVVSLPIIDLSC-GRDEVRRSILEAGKELGFFQVVNH 72

Query: 77  GVSKKVSEETMRIFKEFHAMSGPEKAKECSKDPNKSCRVYTSSENYTKEQIHCWRDALIF 136
           GVSK+V  +   + ++F  +   +KA   S++ +K  R+++ +   T  + + WRD L  
Sbjct: 73  GVSKQVMRDMEGMCEQFFHLPAADKASLYSEERHKPNRLFSGATYDTGGEKY-WRDCLRL 132

Query: 137 NC-HPLEKYVHFWPQKPPKYREVVGAYCMAMRKLVLEILELMSEGLGLGKGYFGGEMSE- 196
            C  P++  ++ WP  P   R+V+  +    R +  E+L L+ EG+G+   YF G++S  
Sbjct: 133 ACPFPVDDSINEWPDTPKGLRDVIEKFTSQTRDVGKELLRLLCEGMGIQADYFEGDLSGG 192

Query: 197 NPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQD-VNGLQVLKDGQWIGVQPIDNAFV 256
           N +L +NHYP CPNP   LG   HCD +LIT+L    VNGL+V   G WI V P  NAFV
Sbjct: 193 NVILNINHYPSCPNPDKALGQPPHCDRNLITLLLPGAVNGLEVSYKGDWIKVDPAPNAFV 252

Query: 257 VNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAMMEPAKCMINEANPPRY 316
           VN G  L+++TNG L++ EHRA+TNS  +R S+   + P  E ++ PAK  +++ NPP Y
Sbjct: 253 VNFGQQLEVVTNGLLKSIEHRAMTNSALARTSVATFIMPTQECLIGPAKEFLSKENPPCY 312

Query: 317 RSLNFKDFQRNY 323
           R+  F+DF R Y
Sbjct: 313 RTTMFRDFMRIY 317

BLAST of CSPI07G15690 vs. Swiss-Prot
Match: DLO2_ARATH (Protein DMR6-LIKE OXYGENASE 2 OS=Arabidopsis thaliana GN=DLO2 PE=2 SV=1)

HSP 1 Score: 227.3 bits (578), Expect = 2.6e-58
Identity = 126/325 (38.77%), Postives = 190/325 (58.46%), Query Frame = 1

Query: 15  VESVPESYVYPPEKRPG-NIVVPMAKAIPVIDLSIR---DRTLLVRKILDASKEFGFFQI 74
           V+ VP +YV P   RP  + V     +IP+IDL      +R  ++ +   A    GFFQI
Sbjct: 15  VDHVPSNYVRPVSDRPKMSEVQTSGDSIPLIDLHDLHGPNRADIINQFAHACSSCGFFQI 74

Query: 75  INHGVSKKVSEETMRIFKEFHAMSGPEKAKECSKDPNKSCRVYTSSENYTKEQIHCWRDA 134
            NHGV ++  ++ M   +EF   S  E+ K  S D  K+ R+ TS  N +KE++  WRD 
Sbjct: 75  KNHGVPEETIKKMMNAAREFFRQSESERVKHYSADTKKTTRLSTSF-NVSKEKVSNWRDF 134

Query: 135 LIFNCHPLEKYVHFWPQKPPKYREVVGAYCMAMRKLVLEILELMSEGLGLGKGYFGGEMS 194
           L  +C+P+E +++ WP  P  +REV   Y  ++R LVL +LE +SE LGL K      + 
Sbjct: 135 LRLHCYPIEDFINEWPSTPISFREVTAEYATSVRALVLTLLEAISESLGLAKDRVSNTIG 194

Query: 195 ENPL-LLVNHYPPCPNPSLTLGLSQHCDPSLITILFQD-VNGLQVLKDGQWIGVQPIDNA 254
           ++   + +N+YP CP P LT GL  H D +LIT+L QD V+GLQV KDG+WI V P+ N 
Sbjct: 195 KHGQHMAINYYPRCPQPELTYGLPGHKDANLITVLLQDEVSGLQVFKDGKWIAVNPVPNT 254

Query: 255 FVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAMMEPAKCMINEA--N 314
           F+VN+G  +Q+I+N K ++  HRAV NS   R S+     P ++A++ PA+ +INE   +
Sbjct: 255 FIVNLGDQMQVISNEKYKSVLHRAVVNSDMERISIPTFYCPSEDAVISPAQELINEEEDS 314

Query: 315 PPRYRSLNFKDFQRNYLPRAVDTKA 332
           P  YR+  + ++   +   A DT++
Sbjct: 315 PAIYRNFTYAEYFEKFWDTAFDTES 338

BLAST of CSPI07G15690 vs. Swiss-Prot
Match: DLO1_ARATH (Protein DMR6-LIKE OXYGENASE 1 OS=Arabidopsis thaliana GN=DLO1 PE=1 SV=1)

HSP 1 Score: 221.1 bits (562), Expect = 1.9e-56
Identity = 123/334 (36.83%), Postives = 191/334 (57.19%), Query Frame = 1

Query: 9   VSNWETVESVPESYVYPPEKRPG-NIVVPMAKAIPVIDLSIRD-----RTLLVRKILDAS 68
           VS++ +   +P +YV P   RP  + V     +IP+IDL  RD     R ++V+++  A 
Sbjct: 11  VSDFASSVHIPSNYVRPISDRPNLSEVESSGDSIPLIDL--RDLHGPNRAVIVQQLASAC 70

Query: 69  KEFGFFQIINHGVSKKVSEETMRIFKEFHAMSGPEKAKECSKDPNKSCRVYTSSENYTKE 128
             +GFFQI NHGV      +   + +EF      E+ K  S DP K+ R+ TS  N   +
Sbjct: 71  STYGFFQIKNHGVPDTTVNKMQTVAREFFHQPESERVKHYSADPTKTTRLSTSF-NVGAD 130

Query: 129 QIHCWRDALIFNCHPLEKYVHFWPQKPPKYREVVGAYCMAMRKLVLEILELMSEGLGLGK 188
           ++  WRD L  +C P+E ++  WP  P  +REV   Y  ++R LVL +LE +SE LGL  
Sbjct: 131 KVLNWRDFLRLHCFPIEDFIEEWPSSPISFREVTAEYATSVRALVLRLLEAISESLGLES 190

Query: 189 GYFGGEMSENPL-LLVNHYPPCPNPSLTLGLSQHCDPSLITILFQD-VNGLQVLKDGQWI 248
            +    + ++   +  N+YPPCP P LT GL  H DP++IT+L QD V+GLQV KD +W+
Sbjct: 191 DHISNILGKHAQHMAFNYYPPCPEPELTYGLPGHKDPTVITVLLQDQVSGLQVFKDDKWV 250

Query: 249 GVQPIDNAFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAMMEPAKC 308
            V PI N F+VNIG  +Q+I+N K ++  HRAV N++  R S+    +P  +A++ PA  
Sbjct: 251 AVSPIPNTFIVNIGDQMQVISNDKYKSVLHRAVVNTENERLSIPTFYFPSTDAVIGPAHE 310

Query: 309 MINEANP-PRYRSLNFKDFQRNYLPRAVDTKAVM 334
           ++NE +    YR+  F ++   +  R++ T + +
Sbjct: 311 LVNEQDSLAIYRTYPFVEYWDKFWNRSLATASCL 341

BLAST of CSPI07G15690 vs. TrEMBL
Match: A0A0A0K5H3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G378370 PE=3 SV=1)

HSP 1 Score: 702.2 bits (1811), Expect = 3.1e-199
Identity = 339/341 (99.41%), Postives = 341/341 (100.00%), Query Frame = 1

Query: 1   MLQGGAKFVSNWETVESVPESYVYPPEKRPGNIVVPMAKAIPVIDLSIRDRTLLVRKILD 60
           MLQGGAKFVSNWETVESVPESYVYPPEKRPGNIVVPMAKAIPVIDLSIRDRTLLVRKILD
Sbjct: 1   MLQGGAKFVSNWETVESVPESYVYPPEKRPGNIVVPMAKAIPVIDLSIRDRTLLVRKILD 60

Query: 61  ASKEFGFFQIINHGVSKKVSEETMRIFKEFHAMSGPEKAKECSKDPNKSCRVYTSSENYT 120
           ASKEFGFFQIINHGVSKKVSEETMRIFKEFHAMSGPEKAKECSKDPN+SCRVYTSSENYT
Sbjct: 61  ASKEFGFFQIINHGVSKKVSEETMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENYT 120

Query: 121 KEQIHCWRDALIFNCHPLEKYVHFWPQKPPKYREVVGAYCMAMRKLVLEILELMSEGLGL 180
           KEQ+HCWRDALIFNCHPLEKYVHFWPQKPPKYREVVGAYCMAMRKLVLEILELMSEGLGL
Sbjct: 121 KEQVHCWRDALIFNCHPLEKYVHFWPQKPPKYREVVGAYCMAMRKLVLEILELMSEGLGL 180

Query: 181 GKGYFGGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWI 240
           GKGYFGGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWI
Sbjct: 181 GKGYFGGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWI 240

Query: 241 GVQPIDNAFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAMMEPAKC 300
           GVQPIDNAFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAMMEPAKC
Sbjct: 241 GVQPIDNAFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAMMEPAKC 300

Query: 301 MINEANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIETNQS 342
           MINEANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIETNQS
Sbjct: 301 MINEANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIETNQS 341

BLAST of CSPI07G15690 vs. TrEMBL
Match: A0A0A0K5H0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G375850 PE=3 SV=1)

HSP 1 Score: 441.0 bits (1133), Expect = 1.3e-120
Identity = 209/335 (62.39%), Postives = 259/335 (77.31%), Query Frame = 1

Query: 7   KFVSNWETVESVPESYVYPPEKRPGNIVVPMAKAIPVIDLSIRDRTLLVRKILDASKEFG 66
           KFVSNW  V SVPE+YVYPPEKR G   + + K+IPVID S  DR LL++++ D ++EFG
Sbjct: 6   KFVSNWGGVVSVPETYVYPPEKRFG---ISVKKSIPVIDWS-DDRALLLKRVHDVTQEFG 65

Query: 67  FFQIINHGVSKKVSEETMRIFKEFHAMSGPEKAKECSKDPNKSCRVYTSSENYTKEQIHC 126
           F Q+INHGV K + EE M+I KEFH MS  +K +ECSKDP+KSC+VYTSS+NY  EQIH 
Sbjct: 66  FVQVINHGVPKTLVEEMMKIMKEFHGMSAEDKERECSKDPDKSCKVYTSSQNYENEQIHL 125

Query: 127 WRDALIFNCHP-LEKYVHFWPQKPPKYREVVGAYCMAMRKLVLEILELMSEGLGLGKGYF 186
           W+D     CHP L+K + FWPQ PPK REVV AY + + K   EILE++SEGLGL KGYF
Sbjct: 126 WKDTFKLRCHPNLDKNLEFWPQNPPKLREVVKAYIVMIEKFASEILEVLSEGLGLEKGYF 185

Query: 187 GGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWIGVQPI 246
            GEMS+NP  L +HYPPCPNPSLT+GLSQH DP+LITILFQDVNGLQ LKDGQWI V P+
Sbjct: 186 RGEMSKNPEFLAHHYPPCPNPSLTVGLSQHADPTLITILFQDVNGLQFLKDGQWIDVDPL 245

Query: 247 DNAFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAMMEPAKCMINEA 306
            +AF+VN+G+V ++I+NGKL+AAEHR VTNS TSRQ+L Y+VYP+++  +EP+KC INEA
Sbjct: 246 PDAFLVNLGYVFEVISNGKLKAAEHRVVTNSTTSRQTLVYVVYPENDVTIEPSKCFINEA 305

Query: 307 NPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIETNQ 341
           NPP YRSL FKDF    + +  D K  M++I  +Q
Sbjct: 306 NPPHYRSLEFKDFYNKLMSKPPDRKTAMEFISFDQ 336

BLAST of CSPI07G15690 vs. TrEMBL
Match: A0A0A0K745_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G375860 PE=3 SV=1)

HSP 1 Score: 438.7 bits (1127), Expect = 6.4e-120
Identity = 207/335 (61.79%), Postives = 260/335 (77.61%), Query Frame = 1

Query: 7   KFVSNWETVESVPESYVYPPEKRPGNIVVPMAKAIPVIDLSIRDRTLLVRKILDASKEFG 66
           KFVSNW+ + SVPE+YV PPEKR G   V + K IPVIDL+  DR LL +K+LD S+EFG
Sbjct: 5   KFVSNWKGIGSVPETYVCPPEKRVG---VSVKKKIPVIDLATEDRDLLSKKVLDVSQEFG 64

Query: 67  FFQIINHGVSKKVSEETMRIFKEFHAMSGPEKAKECSKDPNKSCRVYTSSENYTKEQIHC 126
           FFQ+INHGVSK + EETMR+ +EFHAMS  +K  ECSKDPNKSC +Y+S+ NY KE++H 
Sbjct: 65  FFQVINHGVSKALVEETMRVSREFHAMSSEDKEMECSKDPNKSCLIYSSTPNYEKEELHL 124

Query: 127 WRDALIFNCHP-LEKYVHFWPQKPPKYREVVGAYCMAMRKLVLEILELMSEGLGLGKGYF 186
           W+D+L   C+P L K V FWPQ PPK+REV+ AY + M+K  LEILE++SEGLGL K YF
Sbjct: 125 WKDSLRLICYPNLNKNVQFWPQNPPKFREVMEAYLVVMKKFSLEILEVLSEGLGLEKEYF 184

Query: 187 GGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWIGVQPI 246
            G MS NP  LV+HYPPCPNPSLTLGL++H DP++ITILFQDVNGLQ LKDG+WI V P+
Sbjct: 185 SGGMSNNPEFLVHHYPPCPNPSLTLGLNKHADPTVITILFQDVNGLQFLKDGEWIDVDPL 244

Query: 247 DNAFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAMMEPAKCMINEA 306
            +AF+VN+G+V ++I+NGK++AAEHR VTN+ TSRQSL Y VYP++   +EP+KC INEA
Sbjct: 245 PDAFLVNLGYVFEVISNGKMKAAEHRVVTNTTTSRQSLAYGVYPENNMTIEPSKCFINEA 304

Query: 307 NPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIETNQ 341
           NPP YR+L FKDF      +  D +  M++I  NQ
Sbjct: 305 NPPHYRTLEFKDFVNKLRSKHPDREMAMKFISFNQ 336

BLAST of CSPI07G15690 vs. TrEMBL
Match: A0A0A0K4U9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G376360 PE=3 SV=1)

HSP 1 Score: 426.8 bits (1096), Expect = 2.5e-116
Identity = 201/330 (60.91%), Postives = 254/330 (76.97%), Query Frame = 1

Query: 8   FVSNWETVESVPESYVYPPEKRPGNIVVPMAKAIPVIDLSIRDRTLLVRKILDASKEFGF 67
           FVSNWE VESVPESYV+P EKRPG   + + K IPV+D + +DR LL +KILD++ EFG 
Sbjct: 6   FVSNWEGVESVPESYVWPQEKRPG---IALKKTIPVLDFATQDRALLFQKILDSTHEFGV 65

Query: 68  FQIINHGVSKKVSEETMRIFKEFHAMSGPEKAKECSKDPNKSCRVYTSSENYTKEQIHCW 127
           FQ+INHGVSKK+ EETM +FKEFH+M    K +E SKD NKSC+V+TSS NY  EQ H W
Sbjct: 66  FQVINHGVSKKLVEETMSVFKEFHSMPPQGKRREVSKD-NKSCKVFTSSNNYENEQTHLW 125

Query: 128 RDALIFNCHP-LEKYVHFWPQKPPKYREVVGAYCMAMRKLVLEILELMSEGLGLGKGYFG 187
           RD L   C+P L + +H WPQ PPK REVVGAYC+AM K   EI++L+SEGLGLG+GYF 
Sbjct: 126 RDCLSLTCYPDLHQNIHSWPQNPPKLREVVGAYCIAMEKFSREIIDLISEGLGLGQGYFE 185

Query: 188 GEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWIGVQPID 247
           GEMS    L  NHYP CPNPSLT+GL+QHCD  L+TILFQDV+GLQV KDG WI + PID
Sbjct: 186 GEMSSYRRLTANHYPICPNPSLTMGLNQHCDRDLMTILFQDVSGLQVFKDGHWISIDPID 245

Query: 248 NAFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAMMEPAKCMINEAN 307
           +AFVVN G++L++I+NGKL+A EHR VTN++ SRQS  Y+++P+ E ++EPAKC+INEAN
Sbjct: 246 DAFVVNFGYLLEVISNGKLKAVEHRVVTNAEASRQSFGYVIFPEKEMIIEPAKCLINEAN 305

Query: 308 PPRYRSLNFKDFQRNYLPRAVDTKAVMQYI 337
           PP YRS   +DF R++   + + +  M++I
Sbjct: 306 PPHYRSFKVEDFNRHFFAVSNNNEETMKFI 331

BLAST of CSPI07G15690 vs. TrEMBL
Match: W9S995_9ROSA (Hyoscyamine 6-dioxygenase OS=Morus notabilis GN=L484_009790 PE=3 SV=1)

HSP 1 Score: 402.9 bits (1034), Expect = 3.9e-109
Identity = 186/339 (54.87%), Postives = 258/339 (76.11%), Query Frame = 1

Query: 9   VSNWE-TVESVPESYVYPPEKRPGNIVVPMAKAIPVIDLSIRDR--TLLVRKILDASKEF 68
           VSNW  T  ++P+SY++PPEKRPG +  P+ K IPVIDLS  D   T  + +IL A +E+
Sbjct: 5   VSNWSSTARTLPKSYIFPPEKRPGQLNFPLDKTIPVIDLSNYDHDPTSTIEQILKAGQEY 64

Query: 69  GFFQIINHGVSKKVSEETMRIFKEFHAMSGPEKAKECSKDPNK-SCRVYTSSENYTKEQI 128
           G FQ+INHGVSK++ +ETM++F+EFHAM   +KA+ECSKDPNK SCR+YTSSEN+  E++
Sbjct: 65  GIFQVINHGVSKELVDETMKVFEEFHAMPAKDKARECSKDPNKISCRLYTSSENFPTEEL 124

Query: 129 HCWRDALIFNCHPLEKYVHFWPQKPPKYREVVGAYCMAMRKLVLEILELMSEGLGLGKGY 188
           H WRDAL+  CHPL++++ FWPQ P +YRE+VG Y + +RKL  +ILEL+ +GLGL  GY
Sbjct: 125 HYWRDALVHPCHPLDEHMQFWPQNPIRYREIVGKYTVEVRKLANKILELIIQGLGLSPGY 184

Query: 189 FGGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQ-DVNGLQVLKDGQWIGVQ 248
           F GE+SENP++LVNHYPPCP PSLTLGL++H DPSLITIL Q +V GLQV KDGQW+ V+
Sbjct: 185 FSGELSENPVVLVNHYPPCPEPSLTLGLAKHSDPSLITILLQGNVPGLQVSKDGQWVVVE 244

Query: 249 PIDNAFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAMMEPAKCMIN 308
           P+ +AF+VNIG++LQ+I+NGKL+  EHR VTNS   R + +  +YP ++++++PA  + +
Sbjct: 245 PLPHAFIVNIGYILQVISNGKLKGVEHRVVTNSSVPRTTASLFIYPSNDSLIKPAATLAD 304

Query: 309 EA-NPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIETNQS 342
            + NPP YR+  +KDF+  Y+    D K V ++++   S
Sbjct: 305 ASNNPPLYRAFQYKDFRSYYVSNRADAKKVHEFVDRYNS 343

BLAST of CSPI07G15690 vs. TAIR10
Match: AT5G24530.1 (AT5G24530.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 261.2 bits (666), Expect = 9.2e-70
Identity = 139/340 (40.88%), Postives = 205/340 (60.29%), Query Frame = 1

Query: 6   AKFVSNWETVESVPESYVYPPEKRPGNIVVPMAKAIPVIDLSIRDRTLLVRKILDASKEF 65
           AK +S      ++PE+YV P   RP    V   +  P+IDLS  DR+ L+++I  A   F
Sbjct: 3   AKLISTGFRHTTLPENYVRPISDRPRLSEVSQLEDFPLIDLSSTDRSFLIQQIHQACARF 62

Query: 66  GFFQIINHGVSKKVSEETMRIFKEFHAMSGPEKAKECSKDPNKSCRVYTSSENYTKEQIH 125
           GFFQ+INHGV+K++ +E + + +EF +MS  EK K  S DP K+ R+ TS  N  KE+++
Sbjct: 63  GFFQVINHGVNKQIIDEMVSVAREFFSMSMEEKMKLYSDDPTKTTRLSTSF-NVKKEEVN 122

Query: 126 CWRDALIFNCHPLEKYVHFWPQKPPKYREVVGAYCMAMRKLVLEILELMSEGLGLGKGYF 185
            WRD L  +C+P+ KYV+ WP  PP ++E+V  Y   +R++  +I EL+SE LGL K Y 
Sbjct: 123 NWRDYLRLHCYPIHKYVNEWPSNPPSFKEIVSKYSREVREVGFKIEELISESLGLEKDYM 182

Query: 186 GGEMSE-NPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQD--VNGLQVLKDGQWIGV 245
              + E    + VN+YPPCP P LT GL  H DP+ +TIL QD  V GLQ+L DGQW  V
Sbjct: 183 KKVLGEQGQHMAVNYYPPCPEPELTYGLPAHTDPNALTILLQDTTVCGLQILIDGQWFAV 242

Query: 246 QPIDNAFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAMMEPAKCM- 305
            P  +AFV+NIG  LQ ++NG  ++  HRAVTN++  R S+   + P D A+M PAK + 
Sbjct: 243 NPHPDAFVINIGDQLQALSNGVYKSVWHRAVTNTENPRLSVASFLCPADCAVMSPAKPLW 302

Query: 306 --INEANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIETN 340
              ++   P Y+   + ++ + +  R +D +  ++    N
Sbjct: 303 EAEDDETKPVYKDFTYAEYYKKFWSRNLDQEHCLENFLNN 341

BLAST of CSPI07G15690 vs. TAIR10
Match: AT4G10490.1 (AT4G10490.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 227.3 bits (578), Expect = 1.5e-59
Identity = 126/325 (38.77%), Postives = 190/325 (58.46%), Query Frame = 1

Query: 15  VESVPESYVYPPEKRPG-NIVVPMAKAIPVIDLSIR---DRTLLVRKILDASKEFGFFQI 74
           V+ VP +YV P   RP  + V     +IP+IDL      +R  ++ +   A    GFFQI
Sbjct: 15  VDHVPSNYVRPVSDRPKMSEVQTSGDSIPLIDLHDLHGPNRADIINQFAHACSSCGFFQI 74

Query: 75  INHGVSKKVSEETMRIFKEFHAMSGPEKAKECSKDPNKSCRVYTSSENYTKEQIHCWRDA 134
            NHGV ++  ++ M   +EF   S  E+ K  S D  K+ R+ TS  N +KE++  WRD 
Sbjct: 75  KNHGVPEETIKKMMNAAREFFRQSESERVKHYSADTKKTTRLSTSF-NVSKEKVSNWRDF 134

Query: 135 LIFNCHPLEKYVHFWPQKPPKYREVVGAYCMAMRKLVLEILELMSEGLGLGKGYFGGEMS 194
           L  +C+P+E +++ WP  P  +REV   Y  ++R LVL +LE +SE LGL K      + 
Sbjct: 135 LRLHCYPIEDFINEWPSTPISFREVTAEYATSVRALVLTLLEAISESLGLAKDRVSNTIG 194

Query: 195 ENPL-LLVNHYPPCPNPSLTLGLSQHCDPSLITILFQD-VNGLQVLKDGQWIGVQPIDNA 254
           ++   + +N+YP CP P LT GL  H D +LIT+L QD V+GLQV KDG+WI V P+ N 
Sbjct: 195 KHGQHMAINYYPRCPQPELTYGLPGHKDANLITVLLQDEVSGLQVFKDGKWIAVNPVPNT 254

Query: 255 FVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAMMEPAKCMINEA--N 314
           F+VN+G  +Q+I+N K ++  HRAV NS   R S+     P ++A++ PA+ +INE   +
Sbjct: 255 FIVNLGDQMQVISNEKYKSVLHRAVVNSDMERISIPTFYCPSEDAVISPAQELINEEEDS 314

Query: 315 PPRYRSLNFKDFQRNYLPRAVDTKA 332
           P  YR+  + ++   +   A DT++
Sbjct: 315 PAIYRNFTYAEYFEKFWDTAFDTES 338

BLAST of CSPI07G15690 vs. TAIR10
Match: AT4G10500.1 (AT4G10500.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 221.1 bits (562), Expect = 1.1e-57
Identity = 123/334 (36.83%), Postives = 191/334 (57.19%), Query Frame = 1

Query: 9   VSNWETVESVPESYVYPPEKRPG-NIVVPMAKAIPVIDLSIRD-----RTLLVRKILDAS 68
           VS++ +   +P +YV P   RP  + V     +IP+IDL  RD     R ++V+++  A 
Sbjct: 11  VSDFASSVHIPSNYVRPISDRPNLSEVESSGDSIPLIDL--RDLHGPNRAVIVQQLASAC 70

Query: 69  KEFGFFQIINHGVSKKVSEETMRIFKEFHAMSGPEKAKECSKDPNKSCRVYTSSENYTKE 128
             +GFFQI NHGV      +   + +EF      E+ K  S DP K+ R+ TS  N   +
Sbjct: 71  STYGFFQIKNHGVPDTTVNKMQTVAREFFHQPESERVKHYSADPTKTTRLSTSF-NVGAD 130

Query: 129 QIHCWRDALIFNCHPLEKYVHFWPQKPPKYREVVGAYCMAMRKLVLEILELMSEGLGLGK 188
           ++  WRD L  +C P+E ++  WP  P  +REV   Y  ++R LVL +LE +SE LGL  
Sbjct: 131 KVLNWRDFLRLHCFPIEDFIEEWPSSPISFREVTAEYATSVRALVLRLLEAISESLGLES 190

Query: 189 GYFGGEMSENPL-LLVNHYPPCPNPSLTLGLSQHCDPSLITILFQD-VNGLQVLKDGQWI 248
            +    + ++   +  N+YPPCP P LT GL  H DP++IT+L QD V+GLQV KD +W+
Sbjct: 191 DHISNILGKHAQHMAFNYYPPCPEPELTYGLPGHKDPTVITVLLQDQVSGLQVFKDDKWV 250

Query: 249 GVQPIDNAFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAMMEPAKC 308
            V PI N F+VNIG  +Q+I+N K ++  HRAV N++  R S+    +P  +A++ PA  
Sbjct: 251 AVSPIPNTFIVNIGDQMQVISNDKYKSVLHRAVVNTENERLSIPTFYFPSTDAVIGPAHE 310

Query: 309 MINEANP-PRYRSLNFKDFQRNYLPRAVDTKAVM 334
           ++NE +    YR+  F ++   +  R++ T + +
Sbjct: 311 LVNEQDSLAIYRTYPFVEYWDKFWNRSLATASCL 341

BLAST of CSPI07G15690 vs. TAIR10
Match: AT3G19000.1 (AT3G19000.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 189.5 bits (480), Expect = 3.4e-48
Identity = 117/327 (35.78%), Postives = 170/327 (51.99%), Query Frame = 1

Query: 20  ESYVYPPEKRPGNIVVP-----MAKAIPVIDLSI-----RDRTLLVRKILDASKEFGFFQ 79
           E+++  PE RP   +        +  IP IDLS       D+T + ++I +A K +GFFQ
Sbjct: 6   EAFIQAPEHRPNTHLTNSGDFIFSDEIPTIDLSSLEDTHHDKTAIAKEIAEACKRWGFFQ 65

Query: 80  IINHGVSKKVSEETMRIFKEFHAMSGPEKAKECSKDPNKSCRVYTSSENYTKEQIHCWR- 139
           +INHG+   +     +   EF  ++  EK K    + N    +    E +TK  +  W+ 
Sbjct: 66  VINHGLPSALRHRVEKTAAEFFNLTTEEKRKVKRDEVNP---MGYHDEEHTKN-VRDWKE 125

Query: 140 -------DALIFNCHP------LEKYVHFWPQKPPKYREVVGAYCMAMRKLVLEILELMS 199
                  D+ I    P      L K  + WPQ P  +REV   Y   + KL   +LEL+S
Sbjct: 126 IFDFFLQDSTIVPASPEPEDTELRKLTNQWPQNPSHFREVCQEYAREVEKLAFRLLELVS 185

Query: 200 EGLGLGKGYFGGEMSENPLLL-VNHYPPCPNPSLTLGLSQHCDPSLITILFQD-VNGLQV 259
             LGL      G  +E    L  NHYPPCPNP L LG+ +H D   +T+L QD V GLQV
Sbjct: 186 ISLGLPGDRLTGFFNEQTSFLRFNHYPPCPNPELALGVGRHKDGGALTVLAQDSVGGLQV 245

Query: 260 LK--DGQWIGVQPIDNAFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKD 319
            +  DGQWI V+PI +A ++N+G  +Q+ TN +  +AEHR V N+   R S+ +  +P  
Sbjct: 246 SRRSDGQWIPVKPISDALIINMGNCIQVWTNDEYWSAEHRVVVNTSKERFSIPFFFFPSH 305

BLAST of CSPI07G15690 vs. TAIR10
Match: AT5G51810.1 (AT5G51810.1 gibberellin 20 oxidase 2)

HSP 1 Score: 184.1 bits (466), Expect = 1.4e-46
Identity = 114/341 (33.43%), Postives = 187/341 (54.84%), Query Frame = 1

Query: 18  VPESYVYPPEKRPGNIVVPMAKAIPVIDLSIRDRTLLV-RKILDASKEFGFFQIINHGVS 77
           +P  +++P E++P +I +P    +P IDLS +D TL   R I +A  + GFF ++NHGVS
Sbjct: 42  IPNQFIWPDEEKP-SIDIPELN-VPFIDLSSQDSTLEAPRVIAEACTKHGFFLVVNHGVS 101

Query: 78  KKVSEETMRIFKEFHAM--SGPEKAKECSKDPNKSCRVYTSSENYTKEQIHCWRDALIF- 137
           + +  +  R+ + F  M  +G +KA+   + P +SC   +S       ++  W++ L F 
Sbjct: 102 ESLIADAHRLMESFFDMPLAGKQKAQ---RKPGESCGYASSFTGRFSTKLP-WKETLSFQ 161

Query: 138 --NCHPLEKYVHFW-----PQKPPKYREVVGAYCMAMRKLVLEILELMSEGLGLGKGYFG 197
             N +   + V  +      Q+  ++ +V   YC AM  L L+I+EL+   LG+ + YF 
Sbjct: 162 FSNDNSGSRTVQDYFSDTLGQEFEQFGKVYQDYCEAMSSLSLKIMELLGLSLGVNRDYFR 221

Query: 198 GEMSENPLLL-VNHYPPCPNPSLTLGLSQHCDPSLITILFQD-VNGLQVLKDGQWIGVQP 257
           G   EN  ++ +NHYPPC  P LTLG   HCDPS +TIL QD VNGLQV  D QW  ++P
Sbjct: 222 GFFEENDSIMRLNHYPPCQTPDLTLGTGPHCDPSSLTILHQDHVNGLQVFVDNQWQSIRP 281

Query: 258 IDNAFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAMMEPAKCMINE 317
              AFVVNIG     ++NG  ++  HRAV N +++R+S+ + + PK + +++P   ++ +
Sbjct: 282 NPKAFVVNIGDTFMALSNGIFKSCLHRAVVNRESARKSMAFFLCPKKDKVVKPPSDILEK 341

Query: 318 ANPPRYRSLNFKDF----QRNYLPRAVDTKAVMQYIETNQS 342
               +Y    +  F    Q++Y        +   ++ TN +
Sbjct: 342 MKTRKYPDFTWSMFLEFTQKHYRADVNTLDSFSNWVITNNN 376

BLAST of CSPI07G15690 vs. NCBI nr
Match: gi|449458500|ref|XP_004146985.1| (PREDICTED: hyoscyamine 6-dioxygenase-like [Cucumis sativus])

HSP 1 Score: 702.2 bits (1811), Expect = 4.4e-199
Identity = 339/341 (99.41%), Postives = 341/341 (100.00%), Query Frame = 1

Query: 1   MLQGGAKFVSNWETVESVPESYVYPPEKRPGNIVVPMAKAIPVIDLSIRDRTLLVRKILD 60
           MLQGGAKFVSNWETVESVPESYVYPPEKRPGNIVVPMAKAIPVIDLSIRDRTLLVRKILD
Sbjct: 1   MLQGGAKFVSNWETVESVPESYVYPPEKRPGNIVVPMAKAIPVIDLSIRDRTLLVRKILD 60

Query: 61  ASKEFGFFQIINHGVSKKVSEETMRIFKEFHAMSGPEKAKECSKDPNKSCRVYTSSENYT 120
           ASKEFGFFQIINHGVSKKVSEETMRIFKEFHAMSGPEKAKECSKDPN+SCRVYTSSENYT
Sbjct: 61  ASKEFGFFQIINHGVSKKVSEETMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENYT 120

Query: 121 KEQIHCWRDALIFNCHPLEKYVHFWPQKPPKYREVVGAYCMAMRKLVLEILELMSEGLGL 180
           KEQ+HCWRDALIFNCHPLEKYVHFWPQKPPKYREVVGAYCMAMRKLVLEILELMSEGLGL
Sbjct: 121 KEQVHCWRDALIFNCHPLEKYVHFWPQKPPKYREVVGAYCMAMRKLVLEILELMSEGLGL 180

Query: 181 GKGYFGGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWI 240
           GKGYFGGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWI
Sbjct: 181 GKGYFGGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWI 240

Query: 241 GVQPIDNAFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAMMEPAKC 300
           GVQPIDNAFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAMMEPAKC
Sbjct: 241 GVQPIDNAFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAMMEPAKC 300

Query: 301 MINEANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIETNQS 342
           MINEANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIETNQS
Sbjct: 301 MINEANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIETNQS 341

BLAST of CSPI07G15690 vs. NCBI nr
Match: gi|659100775|ref|XP_008451261.1| (PREDICTED: hyoscyamine 6-dioxygenase-like [Cucumis melo])

HSP 1 Score: 668.7 bits (1724), Expect = 5.4e-189
Identity = 324/341 (95.01%), Postives = 328/341 (96.19%), Query Frame = 1

Query: 1   MLQGGAKFVSNWETVESVPESYVYPPEKRPGNIVVPMAKAIPVIDLSIRDRTLLVRKILD 60
           M QG AKFVSNWET ESVPESYVYPPEKRPGN+VVPMAKAIPVIDLSI DR LLVRKIL 
Sbjct: 1   MQQGDAKFVSNWETAESVPESYVYPPEKRPGNVVVPMAKAIPVIDLSICDRALLVRKILG 60

Query: 61  ASKEFGFFQIINHGVSKKVSEETMRIFKEFHAMSGPEKAKECSKDPNKSCRVYTSSENYT 120
           ASKE GFFQIINHGVSK+VSE TMRIFKEFHAMSGPEKAKECSKDPN+SCRVYTSSENY 
Sbjct: 61  ASKESGFFQIINHGVSKRVSEATMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENYK 120

Query: 121 KEQIHCWRDALIFNCHPLEKYVHFWPQKPPKYREVVGAYCMAMRKLVLEILELMSEGLGL 180
           KEQIHCWRDALIFNCHPLEKYVHFWPQ PPKYREVVGAYC+AMRKLVLEILELMSEGLGL
Sbjct: 121 KEQIHCWRDALIFNCHPLEKYVHFWPQNPPKYREVVGAYCVAMRKLVLEILELMSEGLGL 180

Query: 181 GKGYFGGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWI 240
           GKGY GGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWI
Sbjct: 181 GKGYLGGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWI 240

Query: 241 GVQPIDNAFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAMMEPAKC 300
           GVQPID AFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEA MEPAKC
Sbjct: 241 GVQPIDKAFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEATMEPAKC 300

Query: 301 MINEANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIETNQS 342
           MINEANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIETNQS
Sbjct: 301 MINEANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIETNQS 341

BLAST of CSPI07G15690 vs. NCBI nr
Match: gi|659100769|ref|XP_008451257.1| (PREDICTED: hyoscyamine 6-dioxygenase-like [Cucumis melo])

HSP 1 Score: 450.3 bits (1157), Expect = 3.0e-123
Identity = 214/336 (63.69%), Postives = 266/336 (79.17%), Query Frame = 1

Query: 7   KFVSNWETVESVPESYVYPPEKRPGNIVVPMAKAIPVIDLSIRDRTLLVRKILDASKEFG 66
           KFVSNW+ VESVPE+YVYPPEKR G   V + K IPVID +I DRTLL++K+ D ++EFG
Sbjct: 6   KFVSNWKGVESVPETYVYPPEKRFG---VSVKKTIPVIDWAIDDRTLLLKKVHDVTQEFG 65

Query: 67  FFQIINHGVSKKVSEETMRIFKEFHAMSGPEKAKECSKDPN-KSCRVYTSSENYTKEQIH 126
           F Q+INHGV K + EETM+I KEFHAMS  +K +ECSKDP+ KSCRVY+SS+NY  EQIH
Sbjct: 66  FVQVINHGVPKTLVEETMKILKEFHAMSPEDKERECSKDPDHKSCRVYSSSQNYETEQIH 125

Query: 127 CWRDALIFNCHP-LEKYVHFWPQKPPKYREVVGAYCMAMRKLVLEILELMSEGLGLGKGY 186
            W+D L   CHP L++ V FWPQ PPK+REVV AY + + K  LEILE++SEGLGL KGY
Sbjct: 126 LWKDTLKLTCHPNLDENVQFWPQNPPKFREVVKAYLLKIEKFALEILEVLSEGLGLEKGY 185

Query: 187 FGGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWIGVQP 246
             GEMS+NP L V+HYPPCPNPSLT+GL+QH DP+LITILFQDVNGLQ LKDGQWI V P
Sbjct: 186 LRGEMSKNPELFVHHYPPCPNPSLTIGLNQHADPTLITILFQDVNGLQFLKDGQWIDVDP 245

Query: 247 IDNAFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAMMEPAKCMINE 306
           + NAF+VN+G+VL++I+NGK++A EHR VTN+ TSRQSL Y+VYP ++  +EP+KC INE
Sbjct: 246 LPNAFLVNLGYVLEVISNGKMKAVEHRVVTNTTTSRQSLAYVVYPGNDVNIEPSKCFINE 305

Query: 307 ANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIETNQ 341
           ANPP Y SL FKDF +  + +  D +  +++I  NQ
Sbjct: 306 ANPPHYLSLEFKDFIKKLMSKPPDRETALKFISFNQ 338

BLAST of CSPI07G15690 vs. NCBI nr
Match: gi|449462457|ref|XP_004148957.1| (PREDICTED: hyoscyamine 6-dioxygenase-like [Cucumis sativus])

HSP 1 Score: 441.0 bits (1133), Expect = 1.8e-120
Identity = 209/335 (62.39%), Postives = 259/335 (77.31%), Query Frame = 1

Query: 7   KFVSNWETVESVPESYVYPPEKRPGNIVVPMAKAIPVIDLSIRDRTLLVRKILDASKEFG 66
           KFVSNW  V SVPE+YVYPPEKR G   + + K+IPVID S  DR LL++++ D ++EFG
Sbjct: 6   KFVSNWGGVVSVPETYVYPPEKRFG---ISVKKSIPVIDWS-DDRALLLKRVHDVTQEFG 65

Query: 67  FFQIINHGVSKKVSEETMRIFKEFHAMSGPEKAKECSKDPNKSCRVYTSSENYTKEQIHC 126
           F Q+INHGV K + EE M+I KEFH MS  +K +ECSKDP+KSC+VYTSS+NY  EQIH 
Sbjct: 66  FVQVINHGVPKTLVEEMMKIMKEFHGMSAEDKERECSKDPDKSCKVYTSSQNYENEQIHL 125

Query: 127 WRDALIFNCHP-LEKYVHFWPQKPPKYREVVGAYCMAMRKLVLEILELMSEGLGLGKGYF 186
           W+D     CHP L+K + FWPQ PPK REVV AY + + K   EILE++SEGLGL KGYF
Sbjct: 126 WKDTFKLRCHPNLDKNLEFWPQNPPKLREVVKAYIVMIEKFASEILEVLSEGLGLEKGYF 185

Query: 187 GGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWIGVQPI 246
            GEMS+NP  L +HYPPCPNPSLT+GLSQH DP+LITILFQDVNGLQ LKDGQWI V P+
Sbjct: 186 RGEMSKNPEFLAHHYPPCPNPSLTVGLSQHADPTLITILFQDVNGLQFLKDGQWIDVDPL 245

Query: 247 DNAFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAMMEPAKCMINEA 306
            +AF+VN+G+V ++I+NGKL+AAEHR VTNS TSRQ+L Y+VYP+++  +EP+KC INEA
Sbjct: 246 PDAFLVNLGYVFEVISNGKLKAAEHRVVTNSTTSRQTLVYVVYPENDVTIEPSKCFINEA 305

Query: 307 NPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIETNQ 341
           NPP YRSL FKDF    + +  D K  M++I  +Q
Sbjct: 306 NPPHYRSLEFKDFYNKLMSKPPDRKTAMEFISFDQ 336

BLAST of CSPI07G15690 vs. NCBI nr
Match: gi|700189511|gb|KGN44744.1| (hypothetical protein Csa_7G375860 [Cucumis sativus])

HSP 1 Score: 438.7 bits (1127), Expect = 9.2e-120
Identity = 207/335 (61.79%), Postives = 260/335 (77.61%), Query Frame = 1

Query: 7   KFVSNWETVESVPESYVYPPEKRPGNIVVPMAKAIPVIDLSIRDRTLLVRKILDASKEFG 66
           KFVSNW+ + SVPE+YV PPEKR G   V + K IPVIDL+  DR LL +K+LD S+EFG
Sbjct: 5   KFVSNWKGIGSVPETYVCPPEKRVG---VSVKKKIPVIDLATEDRDLLSKKVLDVSQEFG 64

Query: 67  FFQIINHGVSKKVSEETMRIFKEFHAMSGPEKAKECSKDPNKSCRVYTSSENYTKEQIHC 126
           FFQ+INHGVSK + EETMR+ +EFHAMS  +K  ECSKDPNKSC +Y+S+ NY KE++H 
Sbjct: 65  FFQVINHGVSKALVEETMRVSREFHAMSSEDKEMECSKDPNKSCLIYSSTPNYEKEELHL 124

Query: 127 WRDALIFNCHP-LEKYVHFWPQKPPKYREVVGAYCMAMRKLVLEILELMSEGLGLGKGYF 186
           W+D+L   C+P L K V FWPQ PPK+REV+ AY + M+K  LEILE++SEGLGL K YF
Sbjct: 125 WKDSLRLICYPNLNKNVQFWPQNPPKFREVMEAYLVVMKKFSLEILEVLSEGLGLEKEYF 184

Query: 187 GGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWIGVQPI 246
            G MS NP  LV+HYPPCPNPSLTLGL++H DP++ITILFQDVNGLQ LKDG+WI V P+
Sbjct: 185 SGGMSNNPEFLVHHYPPCPNPSLTLGLNKHADPTVITILFQDVNGLQFLKDGEWIDVDPL 244

Query: 247 DNAFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAMMEPAKCMINEA 306
            +AF+VN+G+V ++I+NGK++AAEHR VTN+ TSRQSL Y VYP++   +EP+KC INEA
Sbjct: 245 PDAFLVNLGYVFEVISNGKMKAAEHRVVTNTTTSRQSLAYGVYPENNMTIEPSKCFINEA 304

Query: 307 NPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIETNQ 341
           NPP YR+L FKDF      +  D +  M++I  NQ
Sbjct: 305 NPPHYRTLEFKDFVNKLRSKHPDREMAMKFISFNQ 336