CSPI07G08420 (gene) Wild cucumber (PI 183967)

NameCSPI07G08420
Typegene
OrganismCucumis sativus (Wild cucumber (PI 183967))
DescriptionRNA-directed DNA polymerase (Reverse transcriptase), Ribonuclease H, putative
LocationChr7 : 6221881 .. 6222870 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATATTTTTATCATGGATCAATGTATATTGCTCTAACTCATCTCCATTGCATGTCTTGTCAGCACCATGGTCGTTTTCTTTGTGGGGAATTGATGTTATTGGACTCATTGATCCTAAAGCCTCAAATGGTCATCGTTTTATTCTTGTAGTCATTAATTACATCGCTAAATGGATAGAGGCGGCATTTTACTGCAATGTTACAAGAGGAGTGGTGCTCAAGTTTATAAAAAAAGAGTTGATCTGTCGTTATGGTCTACCAGAGGGCATTATTACAGATAATGCCAAGAACCTTAATAACAAAATGATGGACGAACTTTGTGAGAAATTCAAGATCAATCACAGAAATTCGACTCCATATCGCCCCAAGATGAATGGGGCAGTTGAGGCAGCCAACAAAAATATTAAAAGAATCATTGAGAAGATGACAACAACATACAAAGATTGGCATGAAATACTACCGTTTGCATTGCATGGATATCACACATCAGTTCGTACTTCAACAGGGGCAACACCATTTTCTTTGGTTTATGGTATGGAAGCTGTCCTACCTTTAGAAGTTGAGATACCTTCGTTGAGAGTTCTCATGGAAGCTAAGTTGGATGAAGCTGAATGGATACGAGGTCATTATGAGCAGTTGAATTTCATTGAAGAAAAACGTTTGACAGCATTAAGTCATGGACAACTTTACCAGAGAAGACTAATGCGAGCATACAATAAAAAGGTACACCCTCGAAGTTTTCGAGAAGGAGATTTAGTGTTAAAAACGATGCTTCTATTTCAAAAGGACCATCGAGGAAAATTGACTCCTAATTATGAAGGCCCGTTCGTGGTAAAAAGGACATTTTCAAGAGGAGCTTTGCTTTTAACCAATATGGATGGTGTTGAGTTAAGAAAATCCGGTGAATTCAGATTATGTTCGAAGATACTATGCATGAGGGCTTTTCATAGAAAATTTTGTAGTGTTGAAAGAAGTCCGGAGTATTTCTAG

mRNA sequence

ATGATATTTTTATCATGGATCAATGTATATTGCTCTAACTCATCTCCATTGCATGTCTTGTCAGCACCATGGTCGTTTTCTTTGTGGGGAATTGATGTTATTGGACTCATTGATCCTAAAGCCTCAAATGGTCATCGTTTTATTCTTGTAGTCATTAATTACATCGCTAAATGGATAGAGGCGGCATTTTACTGCAATGTTACAAGAGGAGTGGTGCTCAAGTTTATAAAAAAAGAGTTGATCTGTCGTTATGGTCTACCAGAGGGCATTATTACAGATAATGCCAAGAACCTTAATAACAAAATGATGGACGAACTTTGTGAGAAATTCAAGATCAATCACAGAAATTCGACTCCATATCGCCCCAAGATGAATGGGGCAGTTGAGGCAGCCAACAAAAATATTAAAAGAATCATTGAGAAGATGACAACAACATACAAAGATTGGCATGAAATACTACCGTTTGCATTGCATGGATATCACACATCAGTTCGTACTTCAACAGGGGCAACACCATTTTCTTTGGTTTATGGTATGGAAGCTGTCCTACCTTTAGAAGTTGAGATACCTTCGTTGAGAGTTCTCATGGAAGCTAAGTTGGATGAAGCTGAATGGATACGAGGTCATTATGAGCAGTTGAATTTCATTGAAGAAAAACGTTTGACAGCATTAAGTCATGGACAACTTTACCAGAGAAGACTAATGCGAGCATACAATAAAAAGGTACACCCTCGAAGTTTTCGAGAAGGAGATTTAGTGTTAAAAACGATGCTTCTATTTCAAAAGGACCATCGAGGAAAATTGACTCCTAATTATGAAGGCCCGTTCGTGGTAAAAAGGACATTTTCAAGAGGAGCTTTGCTTTTAACCAATATGGATGGTGTTGAGTTAAGAAAATCCGGTGAATTCAGATTATGTTCGAAGATACTATGCATGAGGGCTTTTCATAGAAAATTTTGTAGTGTTGAAAGAAGTCCGGAGTATTTCTAG

Coding sequence (CDS)

ATGATATTTTTATCATGGATCAATGTATATTGCTCTAACTCATCTCCATTGCATGTCTTGTCAGCACCATGGTCGTTTTCTTTGTGGGGAATTGATGTTATTGGACTCATTGATCCTAAAGCCTCAAATGGTCATCGTTTTATTCTTGTAGTCATTAATTACATCGCTAAATGGATAGAGGCGGCATTTTACTGCAATGTTACAAGAGGAGTGGTGCTCAAGTTTATAAAAAAAGAGTTGATCTGTCGTTATGGTCTACCAGAGGGCATTATTACAGATAATGCCAAGAACCTTAATAACAAAATGATGGACGAACTTTGTGAGAAATTCAAGATCAATCACAGAAATTCGACTCCATATCGCCCCAAGATGAATGGGGCAGTTGAGGCAGCCAACAAAAATATTAAAAGAATCATTGAGAAGATGACAACAACATACAAAGATTGGCATGAAATACTACCGTTTGCATTGCATGGATATCACACATCAGTTCGTACTTCAACAGGGGCAACACCATTTTCTTTGGTTTATGGTATGGAAGCTGTCCTACCTTTAGAAGTTGAGATACCTTCGTTGAGAGTTCTCATGGAAGCTAAGTTGGATGAAGCTGAATGGATACGAGGTCATTATGAGCAGTTGAATTTCATTGAAGAAAAACGTTTGACAGCATTAAGTCATGGACAACTTTACCAGAGAAGACTAATGCGAGCATACAATAAAAAGGTACACCCTCGAAGTTTTCGAGAAGGAGATTTAGTGTTAAAAACGATGCTTCTATTTCAAAAGGACCATCGAGGAAAATTGACTCCTAATTATGAAGGCCCGTTCGTGGTAAAAAGGACATTTTCAAGAGGAGCTTTGCTTTTAACCAATATGGATGGTGTTGAGTTAAGAAAATCCGGTGAATTCAGATTATGTTCGAAGATACTATGCATGAGGGCTTTTCATAGAAAATTTTGTAGTGTTGAAAGAAGTCCGGAGTATTTCTAG
BLAST of CSPI07G08420 vs. Swiss-Prot
Match: POL_MLVFF (Pol polyprotein OS=Friend murine leukemia virus (isolate FB29) GN=pol PE=3 SV=1)

HSP 1 Score: 88.6 bits (218), Expect = 1.4e-16
Identity = 68/241 (28.22%), Postives = 119/241 (49.38%), Query Frame = 1

Query: 44   GHRFILVVINYIAKWIEAAFYCNVTRGVVLKFIKKELICRYGLPEGIITDNAKNLNNKMM 103
            G++++LV I+  + W+EA      T  VV K + +E+  R+G+P+ + TDN     +K+ 
Sbjct: 931  GYKYLLVFIDTFSGWVEAFPTKKETAKVVTKKLLEEIFPRFGMPQVLGTDNGPAFVSKVS 990

Query: 104  DELCEKFKINHRNSTPYRPKMNGAVEAANKNIKRIIEKMT--TTYKDWHEILPFALHGYH 163
              + +   ++ +    YRP+ +G VE  N+ IK  + K+T  T  +DW  +LP AL+   
Sbjct: 991  QTVADLLGVDWKLHCAYRPQSSGQVERMNRTIKETLTKLTLATGSRDWVLLLPLALYRAR 1050

Query: 164  TSVRTSTGATPFSLVYGMEAVLPLEVEIPSLRVLMEAKLDEAEWIRGHYEQLNFIEEKRL 223
             +     G TP+ ++YG     P  V  P   +   AK+     ++ H + L  ++ +  
Sbjct: 1051 NTPGPH-GLTPYEILYGAP---PPLVNFPDPDM---AKVTHNPSLQAHLQALYLVQHEVW 1110

Query: 224  TALSHGQLYQRRLMRAYNKKVHPRSFREGDLVLKTMLLFQKDHRGK-LTPNYEGPFVVKR 282
              L+    YQ +L    ++ V P  FR GD V      + + H+ K L P ++GP+ V  
Sbjct: 1111 RPLA--AAYQEQL----DRPVVPHPFRVGDTV------WVRRHQTKNLEPRWKGPYTVLL 1152

BLAST of CSPI07G08420 vs. Swiss-Prot
Match: POL_MLVMS (Gag-Pol polyprotein OS=Moloney murine leukemia virus (isolate Shinnick) GN=gag-pol PE=1 SV=4)

HSP 1 Score: 86.7 bits (213), Expect = 5.3e-16
Identity = 67/241 (27.80%), Postives = 119/241 (49.38%), Query Frame = 1

Query: 44   GHRFILVVINYIAKWIEAAFYCNVTRGVVLKFIKKELICRYGLPEGIITDNAKNLNNKMM 103
            G++++LV I+  + WIEA      T  VV K + +E+  R+G+P+ + TDN     +K+ 
Sbjct: 1465 GYKYLLVFIDTFSGWIEAFPTKKETAKVVTKKLLEEIFPRFGMPQVLGTDNGPAFVSKVS 1524

Query: 104  DELCEKFKINHRNSTPYRPKMNGAVEAANKNIKRIIEKMT--TTYKDWHEILPFALHGYH 163
              + +   I+ +    YRP+ +G VE  N+ IK  + K+T  T  +DW  +LP AL+   
Sbjct: 1525 QTVADLLGIDWKLHCAYRPQSSGQVERMNRTIKETLTKLTLATGSRDWVLLLPLALYRAR 1584

Query: 164  TSVRTSTGATPFSLVYGMEAVLPLEVEIPSLRVLMEAKLDEAEWIRGHYEQLNFIEEKRL 223
             +     G TP+ ++YG     P  V  P   +    ++  +  ++ H + L  ++ +  
Sbjct: 1585 NTPGPH-GLTPYEILYGAP---PPLVNFPDPDM---TRVTNSPSLQAHLQALYLVQHEVW 1644

Query: 224  TALSHGQLYQRRLMRAYNKKVHPRSFREGDLVLKTMLLFQKDHRGK-LTPNYEGPFVVKR 282
              L+    YQ +L    ++ V P  +R GD V      + + H+ K L P ++GP+ V  
Sbjct: 1645 RPLA--AAYQEQL----DRPVVPHPYRVGDTV------WVRRHQTKNLEPRWKGPYTVLL 1686

BLAST of CSPI07G08420 vs. Swiss-Prot
Match: POL_MLVCB (Gag-Pol polyprotein (Fragment) OS=Cas-Br-E murine leukemia virus GN=gag-pol PE=3 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 7.0e-16
Identity = 66/241 (27.39%), Postives = 119/241 (49.38%), Query Frame = 1

Query: 44  GHRFILVVINYIAKWIEAAFYCNVTRGVVLKFIKKELICRYGLPEGIITDNAKNLNNKMM 103
           G++++LV ++  + WIEA      T  VV K + +E+  R+G+P+ + TDN     +K+ 
Sbjct: 12  GYKYLLVFVDTFSGWIEAFPTKKETAKVVTKKLLEEIFPRFGMPQVLGTDNGPAFVSKVS 71

Query: 104 DELCEKFKINHRNSTPYRPKMNGAVEAANKNIKRIIEKMT--TTYKDWHEILPFALHGYH 163
             + +   I+ +    YRP+ +G VE  N+ IK  + K+T  T  +DW  +LP AL+   
Sbjct: 72  QTVADLLGIDWKLHCAYRPQSSGQVERMNRTIKETLTKLTLATGSRDWVLLLPLALYRAR 131

Query: 164 TSVRTSTGATPFSLVYGMEAVLPLEVEIPSLRVLMEAKLDEAEWIRGHYEQLNFIEEKRL 223
            +     G TP+ ++YG     P  V  P   +    ++  +  ++ H + L  ++ +  
Sbjct: 132 NTPGPH-GLTPYEILYGAP---PPLVNFPDPDM---TRVTNSPSLQAHLQALYLVQHEVW 191

Query: 224 TALSHGQLYQRRLMRAYNKKVHPRSFREGDLVLKTMLLFQKDHRGK-LTPNYEGPFVVKR 282
             L+    YQ +L    ++ V P  +R GD V      + + H+ K L P ++GP+ V  
Sbjct: 192 RPLA--AAYQEQL----DRPVVPHPYRVGDTV------WVRRHQTKNLEPRWKGPYTVLL 233

BLAST of CSPI07G08420 vs. Swiss-Prot
Match: POL_AVIRE (Pol polyprotein (Fragment) OS=Avian reticuloendotheliosis virus GN=pol PE=3 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 9.4e-13
Identity = 43/152 (28.29%), Postives = 79/152 (51.97%), Query Frame = 1

Query: 29  WGIDVIGLIDPKASNGHRFILVVINYIAKWIEAAFYCNVTRGVVLKFIKKELICRYGLPE 88
           W +D   +I  K   G++++LV+++  + W+EA      T  VV+K +  ++I R+GLP 
Sbjct: 192 WEVDFTEMITAKG--GYKYLLVLVDTFSGWVEAYPAKRETSQVVIKHLILDIIPRFGLPV 251

Query: 89  GIITDNAKNLNNKMMDELCEKFKINHRNSTPYRPKMNGAVEAANKNIKRIIEKMTTTYKD 148
            I +DN      K+  +LCE   ++ +    YRP+ +G VE  N+ +K+ I K+    + 
Sbjct: 252 QIGSDNGPAFVAKVTQQLCEALNVSWKLHCAYRPQSSGQVERMNRTLKKAIAKLEDRDRR 311

Query: 149 WHEILPFALHGYHTSVRTSTGATPFSLVYGME 181
              + P +     T      G +PF ++YG++
Sbjct: 312 GLGLPPPSGFAPGTVYPGREGLSPFEILYGLK 341

BLAST of CSPI07G08420 vs. Swiss-Prot
Match: TF22_SCHPO (Transposon Tf2-2 polyprotein OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=Tf2-2 PE=3 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 1.0e-11
Identity = 57/244 (23.36%), Postives = 112/244 (45.90%), Query Frame = 1

Query: 42   SNGHRFILVVINYIAKW-IEAAFYCNVTRGVVLKFIKKELICRYGLPEGIITDNAKNLNN 101
            S+G+  + VV++  +K  I      ++T     +   + +I  +G P+ II DN     +
Sbjct: 998  SSGYNALFVVVDRFSKMAILVPCTKSITAEQTARMFDQRVIAYFGNPKEIIADNDHIFTS 1057

Query: 102  KMMDELCEKFKINHRNSTPYRPKMNGAVEAANKNIKRIIEKMTTTYKD-WHEILPFALHG 161
            +   +   K+    + S PYRP+ +G  E  N+ +++++  + +T+ + W + +      
Sbjct: 1058 QTWKDFAHKYNFVMKFSLPYRPQTDGQTERTNQTVEKLLRCVCSTHPNTWVDHISLVQQS 1117

Query: 162  YHTSVRTSTGATPFSLVYGMEAVLPLEVEIPSLRVLMEAKLDEAEWIRGHYEQLNFIEEK 221
            Y+ ++ ++T  TPF +V+     L   +E+PS       K DE        E +   +  
Sbjct: 1118 YNNAIHSATQMTPFEIVHRYSPALS-PLELPS----FSDKTDE-----NSQETIQVFQ-- 1177

Query: 222  RLTALSHGQLYQRRLMRAYNKKVHP-RSFREGDLVL----KTMLLFQKDHRGKLTPNYEG 279
              T   H      ++ + ++ K+     F+ GDLV+    KT  L + +   KL P++ G
Sbjct: 1178 --TVKEHLNTNNIKMKKYFDMKIQEIEEFQPGDLVMVKRTKTGFLHKSN---KLAPSFAG 1224

BLAST of CSPI07G08420 vs. TrEMBL
Match: A0A061FRJ8_THECC (RNA-directed DNA polymerase, putative OS=Theobroma cacao GN=TCM_045360 PE=4 SV=1)

HSP 1 Score: 444.1 bits (1141), Expect = 1.5e-121
Identity = 213/288 (73.96%), Postives = 240/288 (83.33%), Query Frame = 1

Query: 14   SSPLHVLSAPWSFSLWGIDVIGLIDPKASNGHRFILVVINYIAKWIEAAFYCNVTRGVVL 73
            ++ LHVL++PW FS+WG+DVIGLI PKASNGHRFILV I+Y  KW+EAA Y NVT+ VV 
Sbjct: 1526 ANSLHVLTSPWPFSMWGMDVIGLITPKASNGHRFILVAIDYFTKWVEAASYANVTQKVVC 1585

Query: 74   KFIKKELICRYGLPEGIITDNAKNLNNKMMDELCEKFKINHRNSTPYRPKMNGAVEAANK 133
            KFI+KE+ICRYGLP+ IITDNA NLN  M+ E+C KFKI H NST YRPKMNGAVEAANK
Sbjct: 1586 KFIQKEIICRYGLPKRIITDNASNLNGSMIKEVCAKFKIKHHNSTSYRPKMNGAVEAANK 1645

Query: 134  NIKRIIEKMTTTYKDWHEILPFALHGYHTSVRTSTGATPFSLVYGMEAVLPLEVEIPSLR 193
            NIKRIIEKMT  YKDWHE LPFALH Y T+VRTSTGATPFSLVYGMEAVLP+EVEIPSLR
Sbjct: 1646 NIKRIIEKMTDIYKDWHEKLPFALHAYRTTVRTSTGATPFSLVYGMEAVLPIEVEIPSLR 1705

Query: 194  VLMEAKLDEAEWIRGHYEQLNFIEEKRLTALSHGQLYQRRLMRAYNKKVHPRSFREGDLV 253
            VL E +L+EAEW+   YEQLN IEEKRLTAL HGQLYQ+R+MRAY+KK H R FREG+LV
Sbjct: 1706 VLKEVQLEEAEWVNARYEQLNLIEEKRLTALCHGQLYQKRMMRAYDKKAHSRQFREGELV 1765

Query: 254  LKTMLLFQKDHRGKLTPNYEGPFVVKRTFSRGALLLTNMDGVELRKSG 302
            LK  L  Q D RGK TPN+EGPFVVK+ FSRGAL+L  MDG+E    G
Sbjct: 1766 LKRTLPNQHDPRGKWTPNWEGPFVVKKAFSRGALILAEMDGMEFSNPG 1813

BLAST of CSPI07G08420 vs. TrEMBL
Match: A0A061DQ75_THECC (RNA-directed DNA polymerase OS=Theobroma cacao GN=TCM_003737 PE=4 SV=1)

HSP 1 Score: 442.2 bits (1136), Expect = 5.6e-121
Identity = 212/283 (74.91%), Postives = 239/283 (84.45%), Query Frame = 1

Query: 14  SSPLHVLSAPWSFSLWGIDVIGLIDPKASNGHRFILVVINYIAKWIEAAFYCNVTRGVVL 73
           ++ LHVL++PW FS+WG+DVIGLI PKASNGH+FILV I+Y  KW+EAA Y NVT+ VV 
Sbjct: 163 ANSLHVLASPWPFSMWGMDVIGLITPKASNGHQFILVAIDYFTKWVEAASYANVTQKVVC 222

Query: 74  KFIKKELICRYGLPEGIITDNAKNLNNKMMDELCEKFKINHRNSTPYRPKMNGAVEAANK 133
           KFI+KE+ICRYGLPE IITDNA NLN  MM E+C KFKI H NSTPYRPKMNGAV+AANK
Sbjct: 223 KFIQKEIICRYGLPERIITDNASNLNGSMMKEVCAKFKIKHHNSTPYRPKMNGAVKAANK 282

Query: 134 NIKRIIEKMTTTYKDWHEILPFALHGYHTSVRTSTGATPFSLVYGMEAVLPLEVEIPSLR 193
           NIKRIIEKMT  YKDWHE LPFALH Y T+VRTSTGATPFSLVYGMEAVLP+EVEIPSLR
Sbjct: 283 NIKRIIEKMTDIYKDWHEKLPFALHAYRTTVRTSTGATPFSLVYGMEAVLPIEVEIPSLR 342

Query: 194 VLMEAKLDEAEWIRGHYEQLNFIEEKRLTALSHGQLYQRRLMRAYNKKVHPRSFREGDLV 253
           VL E +L+EAEW+   YEQLN IEEKRLTAL HGQLYQ+R+MRAY+KK H R FREG+LV
Sbjct: 343 VLKEVQLEEAEWVNARYEQLNLIEEKRLTALCHGQLYQKRMMRAYDKKAHSRQFREGELV 402

Query: 254 LKTMLLFQKDHRGKLTPNYEGPFVVKRTFSRGALLLTNMDGVE 297
           LK +L  Q D RGK TPN+EGPFVVK+ FS GAL+L  MDG E
Sbjct: 403 LKRILPNQHDPRGKWTPNWEGPFVVKKAFSGGALILAEMDGRE 445

BLAST of CSPI07G08420 vs. TrEMBL
Match: A0A061EFX8_THECC (RNA-directed DNA polymerase OS=Theobroma cacao GN=TCM_018990 PE=4 SV=1)

HSP 1 Score: 441.0 bits (1133), Expect = 1.2e-120
Identity = 211/283 (74.56%), Postives = 236/283 (83.39%), Query Frame = 1

Query: 14  SSPLHVLSAPWSFSLWGIDVIGLIDPKASNGHRFILVVINYIAKWIEAAFYCNVTRGVVL 73
           ++ LHVL+ PW FS+WG+DVIGLI PKASNGHRFILV I+Y  KW+EAA Y NVT+ VV 
Sbjct: 89  ANSLHVLAPPWPFSMWGMDVIGLITPKASNGHRFILVAIDYFTKWVEAASYANVTQKVVC 148

Query: 74  KFIKKELICRYGLPEGIITDNAKNLNNKMMDELCEKFKINHRNSTPYRPKMNGAVEAANK 133
           KFI+KE+ICRYGLPE IITDN  NLN  MM E+C KFKI H NSTPYRPKMNGAVEAANK
Sbjct: 149 KFIQKEIICRYGLPERIITDNTSNLNGSMMKEVCAKFKIKHHNSTPYRPKMNGAVEAANK 208

Query: 134 NIKRIIEKMTTTYKDWHEILPFALHGYHTSVRTSTGATPFSLVYGMEAVLPLEVEIPSLR 193
           NIKRIIEKMT  YKDWHE LPFALH Y T+VRTSTGATPFSLVYGMEAVLP+EVEIPSLR
Sbjct: 209 NIKRIIEKMTDIYKDWHEKLPFALHAYRTTVRTSTGATPFSLVYGMEAVLPIEVEIPSLR 268

Query: 194 VLMEAKLDEAEWIRGHYEQLNFIEEKRLTALSHGQLYQRRLMRAYNKKVHPRSFREGDLV 253
           VL E +L+E EW+   YEQLN IEEKRLTAL HGQLYQ+R+MRAY+KK H R FREG+LV
Sbjct: 269 VLKEVQLEETEWVNARYEQLNLIEEKRLTALCHGQLYQKRMMRAYDKKAHSRQFREGELV 328

Query: 254 LKTMLLFQKDHRGKLTPNYEGPFVVKRTFSRGALLLTNMDGVE 297
           LK +L  Q D RGK TPN+EGPFV+K+ FS GAL+L  MDG E
Sbjct: 329 LKRILPNQHDPRGKWTPNWEGPFVIKKAFSGGALILAEMDGRE 371

BLAST of CSPI07G08420 vs. TrEMBL
Match: A0A061FVI6_THECC (RNA-directed DNA polymerase OS=Theobroma cacao GN=TCM_012620 PE=4 SV=1)

HSP 1 Score: 438.3 bits (1126), Expect = 8.0e-120
Identity = 208/283 (73.50%), Postives = 238/283 (84.10%), Query Frame = 1

Query: 15  SPLHVLSAPWSFSLWGIDVIGLIDPKASNGHRFILVVINYIAKWIEAAFYCNVTRGVVLK 74
           +PLHV +APW FS+WG+DVIGLI PKASNGHRFILV I+Y  KW+EAA Y NVT+ VV K
Sbjct: 42  APLHVFTAPWPFSMWGMDVIGLITPKASNGHRFILVAIDYFTKWVEAASYANVTQKVVCK 101

Query: 75  FIKKELICRYGLPEGIITDNAKNLNNKMMDELCEKFKINHRNSTPYRPKMNGAVEAANKN 134
           FI+KE+ICRYGLPE IITDNA NLN  M+ ++C KFKI H NST YRPKMNGAVEAANKN
Sbjct: 102 FIQKEIICRYGLPERIITDNASNLNGAMVKDVCTKFKIKHHNSTTYRPKMNGAVEAANKN 161

Query: 135 IKRIIEKMTTTYKDWHEILPFALHGYHTSVRTSTGATPFSLVYGMEAVLPLEVEIPSLRV 194
           IK+I+EKMT  YKDWHE LPFALH Y TSVRTS GATP+SLVYG EAVLP+EVEIPSLRV
Sbjct: 162 IKKIVEKMTEVYKDWHEKLPFALHAYRTSVRTSIGATPYSLVYGAEAVLPVEVEIPSLRV 221

Query: 195 LMEAKLDEAEWIRGHYEQLNFIEEKRLTALSHGQLYQRRLMRAYNKKVHPRSFREGDLVL 254
           LME +L++AEW+R  YEQLN IEEKRL AL HGQ+YQRR+MRAY KKVHPR FREG+LVL
Sbjct: 222 LMETELEDAEWVRSRYEQLNLIEEKRLAALCHGQMYQRRMMRAYEKKVHPRQFREGELVL 281

Query: 255 KTMLLFQKDHRGKLTPNYEGPFVVKRTFSRGALLLTNMDGVEL 298
           K +L  Q D RGK  PN+EGP+VVK+ FS GAL+L +MDG +L
Sbjct: 282 KRILPNQTDFRGKWMPNWEGPYVVKKAFSGGALILADMDGGDL 324

BLAST of CSPI07G08420 vs. TrEMBL
Match: A0A061EXZ3_THECC (RNA-directed DNA polymerase (Reverse transcriptase), Ribonuclease H, putative OS=Theobroma cacao GN=TCM_024700 PE=4 SV=1)

HSP 1 Score: 437.6 bits (1124), Expect = 1.4e-119
Identity = 210/285 (73.68%), Postives = 240/285 (84.21%), Query Frame = 1

Query: 15   SPLHVLSAPWSFSLWGIDVIGLIDPKASNGHRFILVVINYIAKWIEAAFYCNVTRGVVLK 74
            +PLHV +APW FS+WG+DVIGLI PKASNGHRFILV I+Y  KW+EAA Y NVT+ VV K
Sbjct: 1262 APLHVFTAPWPFSMWGMDVIGLITPKASNGHRFILVAIDYFTKWVEAASYANVTQKVVCK 1321

Query: 75   FIKKELICRYGLPEGIITDNAKNLNNKMMDELCEKFKINHRNSTPYRPKMNGAVEAANKN 134
            FI+KE+ICRYGLPE IITDNA NLN  M+ ++C KFKI H NST YRPKMNGAVEAANKN
Sbjct: 1322 FIQKEIICRYGLPERIITDNASNLNGAMVKDVCAKFKIKHHNSTTYRPKMNGAVEAANKN 1381

Query: 135  IKRIIEKMTTTYKDWHEILPFALHGYHTSVRTSTGATPFSLVYGMEAVLPLEVEIPSLRV 194
            IK+I+EKMT  YKDWHE LPFALH Y TSVRTSTGATP+SLVYG EAVLP+EVEIPSLRV
Sbjct: 1382 IKKIVEKMTEVYKDWHEKLPFALHAYRTSVRTSTGATPYSLVYGAEAVLPVEVEIPSLRV 1441

Query: 195  LMEAKLDEAEWIRGHYEQLNFIEEKRLTALSHGQLYQRRLMRAYNKKVHPRSFREGDLVL 254
            LME KL++AEW+R  YEQLN IEEKRL AL HGQ+YQRR++RAY KKVHPR FREG+LVL
Sbjct: 1442 LMETKLEDAEWVRSRYEQLNLIEEKRLAALCHGQMYQRRMIRAYEKKVHPRQFREGELVL 1501

Query: 255  KTMLLFQKDHRGKLTPNYEGPFVVKRTFSRGA--LLLTNMDGVEL 298
            K +L  Q D RGK  PN+EGP+VVK+ FS GA  L+LT+MDG +L
Sbjct: 1502 KRILPNQTDFRGKWMPNWEGPYVVKKAFSGGALILILTDMDGGDL 1546

BLAST of CSPI07G08420 vs. NCBI nr
Match: gi|828335690|ref|XP_012575472.1| (PREDICTED: LOW QUALITY PROTEIN: uncharacterized protein LOC101513027 [Cicer arietinum])

HSP 1 Score: 460.3 bits (1183), Expect = 2.8e-126
Identity = 220/285 (77.19%), Postives = 246/285 (86.32%), Query Frame = 1

Query: 16   PLHVLSAPWSFSLWGIDVIGLIDPKASNGHRFILVVINYIAKWIEAAFYCNVTRGVVLKF 75
            PL+ LSAPWSFS+WGIDVIG+I+ KASNGHRFILV INY  KW+EAA Y NVT+ VV+KF
Sbjct: 1390 PLNTLSAPWSFSMWGIDVIGMIETKASNGHRFILVAINYFTKWVEAALYANVTKNVVVKF 1449

Query: 76   IKKELICRYGLPEGIITDNAKNLNNKMMDELCEKFKINHRNSTPYRPKMNGAVEAANKNI 135
            IK+ELICRYGLP  IITDNA NLNNKMMDELC  FKI H NS+PYRPKMNGAVEAANKNI
Sbjct: 1450 IKRELICRYGLPSKIITDNATNLNNKMMDELCATFKIQHHNSSPYRPKMNGAVEAANKNI 1509

Query: 136  KRIIEKMTTTYKDWHEILPFALHGYHTSVRTSTGATPFSLVYGMEAVLPLEVEIPSLRVL 195
            K+II+KM  TYKDWHEI PFALHGY TSVRTSTGATPFSLVYGMEAVLP+EVEIPSLRVL
Sbjct: 1510 KKIIQKMVITYKDWHEIFPFALHGYRTSVRTSTGATPFSLVYGMEAVLPIEVEIPSLRVL 1569

Query: 196  MEAKLDEAEWIRGHYEQLNFIEEKRLTALSHGQLYQRRLMRAYNKKVHPRSFREGDLVLK 255
            MEAKL+E+EWI+  ++QLN IEEKRL AL HGQLYQ+RL +AY KK+ PR F+EGDLVLK
Sbjct: 1570 MEAKLEESEWIQTRFDQLNLIEEKRLAALCHGQLYQKRLKKAYEKKIRPREFQEGDLVLK 1629

Query: 256  TMLLFQKDHRGKLTPNYEGPFVVKRTFSRGALLLTNMDGVELRKS 301
             +L  QKD+RGK TPNYEG +VVK+ FS GAL+LTNMDG  L  S
Sbjct: 1630 KILPIQKDYRGKWTPNYEGSYVVKKAFSGGALILTNMDGKYLALS 1674

BLAST of CSPI07G08420 vs. NCBI nr
Match: gi|828327848|ref|XP_012573958.1| (PREDICTED: uncharacterized protein LOC101510858 [Cicer arietinum])

HSP 1 Score: 459.1 bits (1180), Expect = 6.3e-126
Identity = 218/282 (77.30%), Postives = 247/282 (87.59%), Query Frame = 1

Query: 16   PLHVLSAPWSFSLWGIDVIGLIDPKASNGHRFILVVINYIAKWIEAAFYCNVTRGVVLKF 75
            PL+ LSAPW FS+WGIDVIG+I+PKASNGHRFILV I+Y  KW+EAA Y NVT+ VV+KF
Sbjct: 1910 PLNTLSAPWPFSMWGIDVIGMIEPKASNGHRFILVAIDYFTKWVEAASYANVTKNVVVKF 1969

Query: 76   IKKELICRYGLPEGIITDNAKNLNNKMMDELCEKFKINHRNSTPYRPKMNGAVEAANKNI 135
            IK+ELICRYGLP  IITDNA NLNNKMMDELC  FKI H NS+PYRPKMNGAVEAANKNI
Sbjct: 1970 IKRELICRYGLPSKIITDNATNLNNKMMDELCATFKIQHHNSSPYRPKMNGAVEAANKNI 2029

Query: 136  KRIIEKMTTTYKDWHEILPFALHGYHTSVRTSTGATPFSLVYGMEAVLPLEVEIPSLRVL 195
            K+II+KM  TYKDWHE+LPFALHGY TSVRTSTGATPFSLVYGMEAVLP+EVEIPSLRVL
Sbjct: 2030 KKIIQKMVITYKDWHEMLPFALHGYRTSVRTSTGATPFSLVYGMEAVLPIEVEIPSLRVL 2089

Query: 196  MEAKLDEAEWIRGHYEQLNFIEEKRLTALSHGQLYQRRLMRAYNKKVHPRSFREGDLVLK 255
            MEA+L+E+EWI+  + QLN IEEKRL AL HGQLYQ+RL +AY KK+ PR F+EGDLVLK
Sbjct: 2090 MEAELEESEWIQTCFYQLNLIEEKRLAALCHGQLYQKRLKKAYEKKIRPREFQEGDLVLK 2149

Query: 256  TMLLFQKDHRGKLTPNYEGPFVVKRTFSRGALLLTNMDGVEL 298
             +L  QKD+RGK TPNYEGP+VVK+ FS GAL+LTNMDG +L
Sbjct: 2150 KILPIQKDYRGKWTPNYEGPYVVKKAFSGGALILTNMDGKDL 2191

BLAST of CSPI07G08420 vs. NCBI nr
Match: gi|828333967|ref|XP_012575064.1| (PREDICTED: LOW QUALITY PROTEIN: uncharacterized protein LOC101491528 [Cicer arietinum])

HSP 1 Score: 458.4 bits (1178), Expect = 1.1e-125
Identity = 216/282 (76.60%), Postives = 247/282 (87.59%), Query Frame = 1

Query: 16   PLHVLSAPWSFSLWGIDVIGLIDPKASNGHRFILVVINYIAKWIEAAFYCNVTRGVVLKF 75
            PL+ LSAPWSFS+WGIDVIG+I+PKASNGHRFILV I+Y  KW+EAA Y NVT+ VV+KF
Sbjct: 2043 PLNTLSAPWSFSMWGIDVIGMIEPKASNGHRFILVAIDYFTKWVEAASYANVTKNVVVKF 2102

Query: 76   IKKELICRYGLPEGIITDNAKNLNNKMMDELCEKFKINHRNSTPYRPKMNGAVEAANKNI 135
            IK+ELICRYGLP  IITDNA NLNNKMMDELC  FKI H NS+PYRPKMNGAVEAANKNI
Sbjct: 2103 IKRELICRYGLPSKIITDNATNLNNKMMDELCATFKIQHHNSSPYRPKMNGAVEAANKNI 2162

Query: 136  KRIIEKMTTTYKDWHEILPFALHGYHTSVRTSTGATPFSLVYGMEAVLPLEVEIPSLRVL 195
            K+II+KM  TYKDWHE+LPFALHGY TSVRTSTGATPFSLVYGMEAVLP+EVEIPSLRVL
Sbjct: 2163 KKIIQKMVITYKDWHEMLPFALHGYRTSVRTSTGATPFSLVYGMEAVLPIEVEIPSLRVL 2222

Query: 196  MEAKLDEAEWIRGHYEQLNFIEEKRLTALSHGQLYQRRLMRAYNKKVHPRSFREGDLVLK 255
            MEAKL+E+EWI+  ++QLN IEEKRL AL HGQLY++RL +AY K + PR F+EGDLVLK
Sbjct: 2223 MEAKLEESEWIQTRFDQLNLIEEKRLAALCHGQLYKKRLKKAYEKNIRPRQFQEGDLVLK 2282

Query: 256  TMLLFQKDHRGKLTPNYEGPFVVKRTFSRGALLLTNMDGVEL 298
             +L  QKD+R K TPNYEGP++VK+ FS GAL+LTNMDG +L
Sbjct: 2283 KILPIQKDYREKWTPNYEGPYIVKKAFSGGALILTNMDGKDL 2324

BLAST of CSPI07G08420 vs. NCBI nr
Match: gi|828338742|ref|XP_012567583.1| (PREDICTED: LOW QUALITY PROTEIN: uncharacterized protein LOC101488624 [Cicer arietinum])

HSP 1 Score: 454.9 bits (1169), Expect = 1.2e-124
Identity = 216/282 (76.60%), Postives = 247/282 (87.59%), Query Frame = 1

Query: 16   PLHVLSAPWSFSLWGIDVIGLIDPKASNGHRFILVVINYIAKWIEAAFYCNVTRGVVLKF 75
            PL+ LSAPWSFS+WGI+VIG+I+ KASNGH FILV I+Y  KW+EAA Y NVT+ VV+KF
Sbjct: 1823 PLNTLSAPWSFSMWGINVIGMIELKASNGHCFILVAIDYFTKWVEAASYANVTKNVVVKF 1882

Query: 76   IKKELICRYGLPEGIITDNAKNLNNKMMDELCEKFKINHRNSTPYRPKMNGAVEAANKNI 135
            IK+ELICRYGLP  IITDNA NLNNKMMDELC  FKI H NS+PYRPKMNGAVEAANKNI
Sbjct: 1883 IKRELICRYGLPSKIITDNATNLNNKMMDELCATFKIQHHNSSPYRPKMNGAVEAANKNI 1942

Query: 136  KRIIEKMTTTYKDWHEILPFALHGYHTSVRTSTGATPFSLVYGMEAVLPLEVEIPSLRVL 195
            K+II+KM  TYKDWHE+LPFALHGY TSVRTSTGATPFSLVYGMEAVLP+EVEIPSLRVL
Sbjct: 1943 KKIIQKMVITYKDWHEMLPFALHGYRTSVRTSTGATPFSLVYGMEAVLPIEVEIPSLRVL 2002

Query: 196  MEAKLDEAEWIRGHYEQLNFIEEKRLTALSHGQLYQRRLMRAYNKKVHPRSFREGDLVLK 255
            MEAKL+E+EWI+  ++QLN IEEKRL AL HGQLYQ+RL +AY KK+ PR F+EGDLVLK
Sbjct: 2003 MEAKLEESEWIQTRFDQLNLIEEKRLAALCHGQLYQKRLKKAYEKKIRPREFQEGDLVLK 2062

Query: 256  TMLLFQKDHRGKLTPNYEGPFVVKRTFSRGALLLTNMDGVEL 298
             +L  QKD+RGK TPNY+GP+VVK+ FS GAL+LTNMDG +L
Sbjct: 2063 KILPIQKDYRGKWTPNYDGPYVVKKAFSCGALILTNMDGKDL 2104

BLAST of CSPI07G08420 vs. NCBI nr
Match: gi|828329454|ref|XP_012574247.1| (PREDICTED: LOW QUALITY PROTEIN: uncharacterized protein LOC101497477 [Cicer arietinum])

HSP 1 Score: 454.9 bits (1169), Expect = 1.2e-124
Identity = 213/282 (75.53%), Postives = 246/282 (87.23%), Query Frame = 1

Query: 16   PLHVLSAPWSFSLWGIDVIGLIDPKASNGHRFILVVINYIAKWIEAAFYCNVTRGVVLKF 75
            PL+ LSAPW FS+WGIDVIG+I+PKASNGHRFILV ++Y  KW+EA  Y NVT+ VV+KF
Sbjct: 1826 PLNTLSAPWPFSMWGIDVIGMIEPKASNGHRFILVAVDYFTKWVEATSYANVTKNVVVKF 1885

Query: 76   IKKELICRYGLPEGIITDNAKNLNNKMMDELCEKFKINHRNSTPYRPKMNGAVEAANKNI 135
            IK+ELICRYGL   IITDNA NLNNKMMDELC  FKI H NS+PYRPKMNGAVEAANKNI
Sbjct: 1886 IKRELICRYGLLSKIITDNATNLNNKMMDELCVTFKIQHHNSSPYRPKMNGAVEAANKNI 1945

Query: 136  KRIIEKMTTTYKDWHEILPFALHGYHTSVRTSTGATPFSLVYGMEAVLPLEVEIPSLRVL 195
            K+II+KM  TYKDWHE+LPFALHGY TSVRTSTGATPFSLVYGME VLP+EVEIPSLRVL
Sbjct: 1946 KKIIQKMVITYKDWHEMLPFALHGYRTSVRTSTGATPFSLVYGMEVVLPIEVEIPSLRVL 2005

Query: 196  MEAKLDEAEWIRGHYEQLNFIEEKRLTALSHGQLYQRRLMRAYNKKVHPRSFREGDLVLK 255
            ME+KL+E+EW++  ++QLN IEEKRL AL HGQLYQ+RL +AY KK++PR F+EGDLVLK
Sbjct: 2006 MESKLEESEWVQTRFDQLNLIEEKRLAALCHGQLYQKRLKKAYEKKIYPREFQEGDLVLK 2065

Query: 256  TMLLFQKDHRGKLTPNYEGPFVVKRTFSRGALLLTNMDGVEL 298
             +L  QKD+RGK TPNYEGP+VVK+ FS GAL+LTNMDG +L
Sbjct: 2066 KILPIQKDYRGKWTPNYEGPYVVKKAFSGGALILTNMDGKDL 2107

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
POL_MLVFF1.4e-1628.22Pol polyprotein OS=Friend murine leukemia virus (isolate FB29) GN=pol PE=3 SV=1[more]
POL_MLVMS5.3e-1627.80Gag-Pol polyprotein OS=Moloney murine leukemia virus (isolate Shinnick) GN=gag-p... [more]
POL_MLVCB7.0e-1627.39Gag-Pol polyprotein (Fragment) OS=Cas-Br-E murine leukemia virus GN=gag-pol PE=3... [more]
POL_AVIRE9.4e-1328.29Pol polyprotein (Fragment) OS=Avian reticuloendotheliosis virus GN=pol PE=3 SV=1[more]
TF22_SCHPO1.0e-1123.36Transposon Tf2-2 polyprotein OS=Schizosaccharomyces pombe (strain 972 / ATCC 248... [more]
Match NameE-valueIdentityDescription
A0A061FRJ8_THECC1.5e-12173.96RNA-directed DNA polymerase, putative OS=Theobroma cacao GN=TCM_045360 PE=4 SV=1[more]
A0A061DQ75_THECC5.6e-12174.91RNA-directed DNA polymerase OS=Theobroma cacao GN=TCM_003737 PE=4 SV=1[more]
A0A061EFX8_THECC1.2e-12074.56RNA-directed DNA polymerase OS=Theobroma cacao GN=TCM_018990 PE=4 SV=1[more]
A0A061FVI6_THECC8.0e-12073.50RNA-directed DNA polymerase OS=Theobroma cacao GN=TCM_012620 PE=4 SV=1[more]
A0A061EXZ3_THECC1.4e-11973.68RNA-directed DNA polymerase (Reverse transcriptase), Ribonuclease H, putative OS... [more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
gi|828335690|ref|XP_012575472.1|2.8e-12677.19PREDICTED: LOW QUALITY PROTEIN: uncharacterized protein LOC101513027 [Cicer arie... [more]
gi|828327848|ref|XP_012573958.1|6.3e-12677.30PREDICTED: uncharacterized protein LOC101510858 [Cicer arietinum][more]
gi|828333967|ref|XP_012575064.1|1.1e-12576.60PREDICTED: LOW QUALITY PROTEIN: uncharacterized protein LOC101491528 [Cicer arie... [more]
gi|828338742|ref|XP_012567583.1|1.2e-12476.60PREDICTED: LOW QUALITY PROTEIN: uncharacterized protein LOC101488624 [Cicer arie... [more]
gi|828329454|ref|XP_012574247.1|1.2e-12475.53PREDICTED: LOW QUALITY PROTEIN: uncharacterized protein LOC101497477 [Cicer arie... [more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR001584Integrase_cat-core
IPR012337RNaseH-like_sf
Vocabulary: Biological Process
TermDefinition
GO:0015074DNA integration
Vocabulary: Molecular Function
TermDefinition
GO:0003676nucleic acid binding
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015074 DNA integration
biological_process GO:0051252 regulation of RNA metabolic process
biological_process GO:0006278 RNA-dependent DNA biosynthetic process
biological_process GO:0090502 RNA phosphodiester bond hydrolysis, endonucleolytic
cellular_component GO:0005575 cellular_component
molecular_function GO:0005544 calcium-dependent phospholipid binding
molecular_function GO:0005509 calcium ion binding
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0003964 RNA-directed DNA polymerase activity
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI07G08420.1CSPI07G08420.1mRNA


Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001584Integrase, catalytic corePFAMPF00665rvecoord: 26..137
score: 1.4
IPR001584Integrase, catalytic corePROFILEPS50994INTEGRASEcoord: 19..180
score: 20
IPR012337Ribonuclease H-like domainGENE3DG3DSA:3.30.420.10coord: 26..184
score: 6.5
IPR012337Ribonuclease H-like domainunknownSSF53098Ribonuclease H-likecoord: 24..186
score: 9.54
NoneNo IPR availablePANTHERPTHR24559FAMILY NOT NAMEDcoord: 16..278
score: 1.2

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None