CSPI06G21950 (gene) Wild cucumber (PI 183967)

NameCSPI06G21950
Typegene
OrganismCucumis sativus (Wild cucumber (PI 183967))
DescriptionAntifungal protein ginkbilobin-2
LocationChr6 : 19913644 .. 19914048 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATTGGCTCTAGTCTGAAAATTTCAGTGGCTTTTCTCGCCTTCTTTGCGCTGTCTATTATGGTGGTGAGCATTCCGAACACTGGAGTGAGGTCAGTTTTGTGCAATTCCGGAACTTTCACAGGGGGGGATCCTTTTACAGTGAGTCTGGATTACGTTCTAAAAGAACTGCAGAGTGCAACTCCCACTGTAAAGAACTATGATTTCTACAACATATCCCCTTATCCTAATGCCTTCGCTTATGGTCATGCTTCCTGCAATCAGAATCTCACAACCTCAGACTGCACTACTTGTCTTGAGGCTGCTAAGAACAACATGCTGGGCTCGTGTCAAATGAGAATTGGGGGTCGTTCAGTGCTCAATGACTGTACAATTAGGTATGAGCAATACCCATTTGATGATTAG

mRNA sequence

ATGATTGGCTCTAGTCTGAAAATTTCAGTGGCTTTTCTCGCCTTCTTTGCGCTGTCTATTATGGTGGTGAGCATTCCGAACACTGGAGTGAGGTCAGTTTTGTGCAATTCCGGAACTTTCACAGGGGGGGATCCTTTTACAGTGAGTCTGGATTACGTTCTAAAAGAACTGCAGAGTGCAACTCCCACTGTAAAGAACTATGATTTCTACAACATATCCCCTTATCCTAATGCCTTCGCTTATGGTCATGCTTCCTGCAATCAGAATCTCACAACCTCAGACTGCACTACTTGTCTTGAGGCTGCTAAGAACAACATGCTGGGCTCGTGTCAAATGAGAATTGGGGGTCGTTCAGTGCTCAATGACTGTACAATTAGGTATGAGCAATACCCATTTGATGATTAG

Coding sequence (CDS)

ATGATTGGCTCTAGTCTGAAAATTTCAGTGGCTTTTCTCGCCTTCTTTGCGCTGTCTATTATGGTGGTGAGCATTCCGAACACTGGAGTGAGGTCAGTTTTGTGCAATTCCGGAACTTTCACAGGGGGGGATCCTTTTACAGTGAGTCTGGATTACGTTCTAAAAGAACTGCAGAGTGCAACTCCCACTGTAAAGAACTATGATTTCTACAACATATCCCCTTATCCTAATGCCTTCGCTTATGGTCATGCTTCCTGCAATCAGAATCTCACAACCTCAGACTGCACTACTTGTCTTGAGGCTGCTAAGAACAACATGCTGGGCTCGTGTCAAATGAGAATTGGGGGTCGTTCAGTGCTCAATGACTGTACAATTAGGTATGAGCAATACCCATTTGATGATTAG
BLAST of CSPI06G21950 vs. Swiss-Prot
Match: GNK2_GINBI (Antifungal protein ginkbilobin-2 OS=Ginkgo biloba GN=GNK2 PE=1 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 1.2e-11
Identity = 44/133 (33.08%), Postives = 66/133 (49.62%), Query Frame = 1

Query: 5   SLKISVAFLAFFALS---IMVVSIPNTGVRSVLCNSGTFTGGDPFTVSLDYVLKELQSAT 64
           +++++ AF+  FAL+   +++    NT   S  CN+     G PF  +L  +L +L+  T
Sbjct: 3   TMRMNSAFILAFALAAAMLILTEAANTAFVSSACNTQKIPSGSPFNRNLRAMLADLRQNT 62

Query: 65  PTVKNYDFYNISPYPNAF--AYGHASCNQNLTTSDCTTCLEAAKNNMLGSCQMRIGGRSV 124
                YD+            AYG A+C Q+++ SDCT CL    N +   C   IG R  
Sbjct: 63  -AFSGYDYKTSRAGSGGAPTAYGRATCKQSISQSDCTACLSNLVNRIFSICNNAIGARVQ 122

Query: 125 LNDCTIRYEQYPF 133
           L DC I+YEQ  F
Sbjct: 123 LVDCFIQYEQRSF 134

BLAST of CSPI06G21950 vs. TrEMBL
Match: A0A0A0KGA2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G409950 PE=4 SV=1)

HSP 1 Score: 272.3 bits (695), Expect = 3.1e-70
Identity = 133/134 (99.25%), Postives = 134/134 (100.00%), Query Frame = 1

Query: 1   MIGSSLKISVAFLAFFALSIMVVSIPNTGVRSVLCNSGTFTGGDPFTVSLDYVLKELQSA 60
           MIGSSLKISVAFLAFFALSIMVVSIPNTGVRSVLCNSGTFTGGDPFTVSLDYVLKELQSA
Sbjct: 1   MIGSSLKISVAFLAFFALSIMVVSIPNTGVRSVLCNSGTFTGGDPFTVSLDYVLKELQSA 60

Query: 61  TPTVKNYDFYNISPYPNAFAYGHASCNQNLTTSDCTTCLEAAKNNMLGSCQMRIGGRSVL 120
           TPTVKNYDFYNISPYPNAFAYGHASCNQNL+TSDCTTCLEAAKNNMLGSCQMRIGGRSVL
Sbjct: 61  TPTVKNYDFYNISPYPNAFAYGHASCNQNLSTSDCTTCLEAAKNNMLGSCQMRIGGRSVL 120

Query: 121 NDCTIRYEQYPFDD 135
           NDCTIRYEQYPFDD
Sbjct: 121 NDCTIRYEQYPFDD 134

BLAST of CSPI06G21950 vs. TrEMBL
Match: M5W194_PRUPE (Uncharacterized protein (Fragment) OS=Prunus persica GN=PRUPE_ppa025382mg PE=4 SV=1)

HSP 1 Score: 186.0 bits (471), Expect = 2.9e-44
Identity = 88/125 (70.40%), Postives = 101/125 (80.80%), Query Frame = 1

Query: 8   ISVAFLAFFALSIMVVSIPNTGVRSVLCNSGTFTGGDPFTVSLDYVLKELQSATPTVKNY 67
           I V F+ FF ++    S P+T + SVLCNSG +T GDPF VSL YVLKEL++AT T KNY
Sbjct: 2   IVVVFIGFFGVA---TSTPDTNITSVLCNSGVYTAGDPFAVSLAYVLKELETATSTHKNY 61

Query: 68  DFYNISPYPNAFAYGHASCNQNLTTSDCTTCLEAAKNNMLGSCQMRIGGRSVLNDCTIRY 127
           DFY+ISPYPNAFAYGHASCNQN+TT DCTTCL AAK +MLG+CQ  IGGR+VL DC IRY
Sbjct: 62  DFYDISPYPNAFAYGHASCNQNITTPDCTTCLGAAKTDMLGTCQNAIGGRAVLRDCAIRY 121

Query: 128 EQYPF 133
           EQYPF
Sbjct: 122 EQYPF 123

BLAST of CSPI06G21950 vs. TrEMBL
Match: A0A067JPK6_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_21945 PE=4 SV=1)

HSP 1 Score: 185.7 bits (470), Expect = 3.8e-44
Identity = 89/128 (69.53%), Postives = 101/128 (78.91%), Query Frame = 1

Query: 7   KISVAFLAFFALSIMVVSIPNTGVRSVLCNSGTFTGGDPFTVSLDYVLKELQSATPTVKN 66
           KI + F   F  S  V SIPNT V +VLCNSG ++ GDPF +SLDYV+KEL++ TPT KN
Sbjct: 71  KIIIVFFLQFC-SNRVTSIPNTQVLTVLCNSGVYSKGDPFGISLDYVVKELETVTPTTKN 130

Query: 67  YDFYNISPYPNAFAYGHASCNQNLTTSDCTTCLEAAKNNMLGSCQMRIGGRSVLNDCTIR 126
           YDFYNISPYPNAFAYGHA CNQNLT  DC TCL AAK+NM  +CQ RIG RSVL+DC+IR
Sbjct: 131 YDFYNISPYPNAFAYGHAVCNQNLTNLDCETCLGAAKSNMFSTCQNRIGARSVLHDCSIR 190

Query: 127 YEQYPFDD 135
           YEQYPF D
Sbjct: 191 YEQYPFTD 197

BLAST of CSPI06G21950 vs. TrEMBL
Match: B9H872_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0005s22990g PE=4 SV=2)

HSP 1 Score: 180.6 bits (457), Expect = 1.2e-42
Identity = 81/133 (60.90%), Postives = 103/133 (77.44%), Query Frame = 1

Query: 2   IGSSLKISVAFLAFFALSIMVVSIPNTGVRSVLCNSGTFTGGDPFTVSLDYVLKELQSAT 61
           +G   K+++ F+ FF   ++  S+P+T V +VLCNSG ++ GDPF  SL YVL E+++ T
Sbjct: 7   MGFPPKLALVFIGFFGWCVIATSVPDTNVTTVLCNSGVYSKGDPFGTSLAYVLAEIEAVT 66

Query: 62  PTVKNYDFYNISPYPNAFAYGHASCNQNLTTSDCTTCLEAAKNNMLGSCQMRIGGRSVLN 121
           PT KNYD++NISPYPNA AYGHA+CNQNLT+SDC++CL  AK  M  SCQ RIG RSVL+
Sbjct: 67  PTSKNYDYFNISPYPNAVAYGHAACNQNLTSSDCSSCLGTAKTAMFASCQSRIGARSVLH 126

Query: 122 DCTIRYEQYPFDD 135
           DCTIRYEQYPF D
Sbjct: 127 DCTIRYEQYPFSD 139

BLAST of CSPI06G21950 vs. TrEMBL
Match: A0A0D2P8H3_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_007G136000 PE=4 SV=1)

HSP 1 Score: 178.3 bits (451), Expect = 6.1e-42
Identity = 81/128 (63.28%), Postives = 101/128 (78.91%), Query Frame = 1

Query: 7   KISVAFLAFFALSIMVVSIPNTGVRSVLCNSGTFTGGDPFTVSLDYVLKELQSATPTVKN 66
           K + A +    L   V+S+P+T + SVLCNSG+++ GDPF  SL +VL++L++ TP  K 
Sbjct: 6   KQAAAMIGLLLLCSNVISVPDTSITSVLCNSGSYSEGDPFATSLAFVLQDLETLTPERKG 65

Query: 67  YDFYNISPYPNAFAYGHASCNQNLTTSDCTTCLEAAKNNMLGSCQMRIGGRSVLNDCTIR 126
           YD++NISPYPNAFAYGHA+CNQNLTTSDC TCL AAK  +L +CQ RIG RSVL+DCTIR
Sbjct: 66  YDYFNISPYPNAFAYGHAACNQNLTTSDCATCLGAAKTVLLATCQSRIGSRSVLHDCTIR 125

Query: 127 YEQYPFDD 135
           YEQYPFDD
Sbjct: 126 YEQYPFDD 133

BLAST of CSPI06G21950 vs. TAIR10
Match: AT4G23140.2 (AT4G23140.2 cysteine-rich RLK (RECEPTOR-like protein kinase) 6)

HSP 1 Score: 50.8 bits (120), Expect = 7.5e-07
Identity = 40/121 (33.06%), Postives = 59/121 (48.76%), Query Frame = 1

Query: 19  SIMVVSIPNTGVRSVLCNSGTFTGG----DPFTVSLDYVLKEL--QSATPTVKNYDFYN- 78
           +I+  +I N G   +L N+ T +      D FT  +   + E   ++A  + K Y     
Sbjct: 131 NILSTAITNEG-EFILSNTNTISPNQKQIDGFTSFVSSTMSEAAGKAANSSRKLYTVNTE 190

Query: 79  ISPYPNAFAYGHASCNQNLTTSDCTTCLEAAKNNMLGSCQMRIGGRSVLNDCTIRYEQYP 133
           ++ Y N   YG   C  +LT +DC +CL+++ N M  S   RIG R     CT RYE YP
Sbjct: 191 LTAYQNL--YGLLQCTPDLTRADCLSCLQSSINGMALS---RIGARLYWPSCTARYELYP 245

BLAST of CSPI06G21950 vs. TAIR10
Match: AT5G48540.1 (AT5G48540.1 receptor-like protein kinase-related family protein)

HSP 1 Score: 50.4 bits (119), Expect = 9.8e-07
Identity = 30/101 (29.70%), Postives = 51/101 (50.50%), Query Frame = 1

Query: 34  LCNSGT-FTGGDPFTVSLDYVLKELQSATPTVKNYDFYNISPYPNAF-AYGHASCNQNLT 93
           LCN+ +  +     + ++D +L  L S TP+ K +     S Y N    YG A C  +++
Sbjct: 27  LCNTNSNISASSQVSKNIDSLLATLVSKTPS-KGFKTTTSSSYNNKEKVYGLAQCRGDIS 86

Query: 94  TSDCTTCLEAAKNNMLGSCQMRIGGRSVLNDCTIRYEQYPF 133
            +DC+TC++ A   +   CQ +   R + + C +RY Q  F
Sbjct: 87  NTDCSTCIQDAAKKIREVCQNQSDSRILYDFCFLRYSQENF 126

BLAST of CSPI06G21950 vs. TAIR10
Match: AT4G05200.1 (AT4G05200.1 cysteine-rich RLK (RECEPTOR-like protein kinase) 25)

HSP 1 Score: 50.1 bits (118), Expect = 1.3e-06
Identity = 20/52 (38.46%), Postives = 26/52 (50.00%), Query Frame = 1

Query: 81  YGHASCNQNLTTSDCTTCLEAAKNNMLGSCQMRIGGRSVLNDCTIRYEQYPF 133
           Y    C  +LT  DC +CL    N +   C   +GGR +   C+ RYE YPF
Sbjct: 196 YSLVQCTPDLTNQDCESCLRQVINYLPRCCDRSVGGRVIAPSCSFRYELYPF 247

BLAST of CSPI06G21950 vs. NCBI nr
Match: gi|700192685|gb|KGN47889.1| (hypothetical protein Csa_6G409950 [Cucumis sativus])

HSP 1 Score: 272.3 bits (695), Expect = 4.5e-70
Identity = 133/134 (99.25%), Postives = 134/134 (100.00%), Query Frame = 1

Query: 1   MIGSSLKISVAFLAFFALSIMVVSIPNTGVRSVLCNSGTFTGGDPFTVSLDYVLKELQSA 60
           MIGSSLKISVAFLAFFALSIMVVSIPNTGVRSVLCNSGTFTGGDPFTVSLDYVLKELQSA
Sbjct: 1   MIGSSLKISVAFLAFFALSIMVVSIPNTGVRSVLCNSGTFTGGDPFTVSLDYVLKELQSA 60

Query: 61  TPTVKNYDFYNISPYPNAFAYGHASCNQNLTTSDCTTCLEAAKNNMLGSCQMRIGGRSVL 120
           TPTVKNYDFYNISPYPNAFAYGHASCNQNL+TSDCTTCLEAAKNNMLGSCQMRIGGRSVL
Sbjct: 61  TPTVKNYDFYNISPYPNAFAYGHASCNQNLSTSDCTTCLEAAKNNMLGSCQMRIGGRSVL 120

Query: 121 NDCTIRYEQYPFDD 135
           NDCTIRYEQYPFDD
Sbjct: 121 NDCTIRYEQYPFDD 134

BLAST of CSPI06G21950 vs. NCBI nr
Match: gi|645259245|ref|XP_008235269.1| (PREDICTED: antifungal protein ginkbilobin-2-like [Prunus mume])

HSP 1 Score: 186.4 bits (472), Expect = 3.2e-44
Identity = 89/127 (70.08%), Postives = 102/127 (80.31%), Query Frame = 1

Query: 8   ISVAFLAFFALSIMVVSIPNTGVRSVLCNSGTFTGGDPFTVSLDYVLKELQSATPTVKNY 67
           I V FL FF ++    S P+T + SVLCNSG +T GDPF VSL YVL+EL++AT T KNY
Sbjct: 13  IVVFFLGFFGVA---TSTPDTNITSVLCNSGVYTAGDPFAVSLAYVLQELETATSTHKNY 72

Query: 68  DFYNISPYPNAFAYGHASCNQNLTTSDCTTCLEAAKNNMLGSCQMRIGGRSVLNDCTIRY 127
           DFY+ISPYPNAFAYGHASCNQN+TT DCTTCL AAK +MLG+CQ  IGGR+VL DC IRY
Sbjct: 73  DFYDISPYPNAFAYGHASCNQNITTPDCTTCLGAAKTDMLGTCQNAIGGRAVLRDCAIRY 132

Query: 128 EQYPFDD 135
           EQYPF D
Sbjct: 133 EQYPFTD 136

BLAST of CSPI06G21950 vs. NCBI nr
Match: gi|595791225|ref|XP_007199361.1| (hypothetical protein PRUPE_ppa025382mg, partial [Prunus persica])

HSP 1 Score: 186.0 bits (471), Expect = 4.2e-44
Identity = 88/125 (70.40%), Postives = 101/125 (80.80%), Query Frame = 1

Query: 8   ISVAFLAFFALSIMVVSIPNTGVRSVLCNSGTFTGGDPFTVSLDYVLKELQSATPTVKNY 67
           I V F+ FF ++    S P+T + SVLCNSG +T GDPF VSL YVLKEL++AT T KNY
Sbjct: 2   IVVVFIGFFGVA---TSTPDTNITSVLCNSGVYTAGDPFAVSLAYVLKELETATSTHKNY 61

Query: 68  DFYNISPYPNAFAYGHASCNQNLTTSDCTTCLEAAKNNMLGSCQMRIGGRSVLNDCTIRY 127
           DFY+ISPYPNAFAYGHASCNQN+TT DCTTCL AAK +MLG+CQ  IGGR+VL DC IRY
Sbjct: 62  DFYDISPYPNAFAYGHASCNQNITTPDCTTCLGAAKTDMLGTCQNAIGGRAVLRDCAIRY 121

Query: 128 EQYPF 133
           EQYPF
Sbjct: 122 EQYPF 123

BLAST of CSPI06G21950 vs. NCBI nr
Match: gi|802792380|ref|XP_012092263.1| (PREDICTED: antifungal protein ginkbilobin-2-like [Jatropha curcas])

HSP 1 Score: 185.7 bits (470), Expect = 5.5e-44
Identity = 89/128 (69.53%), Postives = 101/128 (78.91%), Query Frame = 1

Query: 7   KISVAFLAFFALSIMVVSIPNTGVRSVLCNSGTFTGGDPFTVSLDYVLKELQSATPTVKN 66
           KI + F   F  S  V SIPNT V +VLCNSG ++ GDPF +SLDYV+KEL++ TPT KN
Sbjct: 10  KIIIVFFLQFC-SNRVTSIPNTQVLTVLCNSGVYSKGDPFGISLDYVVKELETVTPTTKN 69

Query: 67  YDFYNISPYPNAFAYGHASCNQNLTTSDCTTCLEAAKNNMLGSCQMRIGGRSVLNDCTIR 126
           YDFYNISPYPNAFAYGHA CNQNLT  DC TCL AAK+NM  +CQ RIG RSVL+DC+IR
Sbjct: 70  YDFYNISPYPNAFAYGHAVCNQNLTNLDCETCLGAAKSNMFSTCQNRIGARSVLHDCSIR 129

Query: 127 YEQYPFDD 135
           YEQYPF D
Sbjct: 130 YEQYPFTD 136

BLAST of CSPI06G21950 vs. NCBI nr
Match: gi|643704410|gb|KDP21474.1| (hypothetical protein JCGZ_21945 [Jatropha curcas])

HSP 1 Score: 185.7 bits (470), Expect = 5.5e-44
Identity = 89/128 (69.53%), Postives = 101/128 (78.91%), Query Frame = 1

Query: 7   KISVAFLAFFALSIMVVSIPNTGVRSVLCNSGTFTGGDPFTVSLDYVLKELQSATPTVKN 66
           KI + F   F  S  V SIPNT V +VLCNSG ++ GDPF +SLDYV+KEL++ TPT KN
Sbjct: 71  KIIIVFFLQFC-SNRVTSIPNTQVLTVLCNSGVYSKGDPFGISLDYVVKELETVTPTTKN 130

Query: 67  YDFYNISPYPNAFAYGHASCNQNLTTSDCTTCLEAAKNNMLGSCQMRIGGRSVLNDCTIR 126
           YDFYNISPYPNAFAYGHA CNQNLT  DC TCL AAK+NM  +CQ RIG RSVL+DC+IR
Sbjct: 131 YDFYNISPYPNAFAYGHAVCNQNLTNLDCETCLGAAKSNMFSTCQNRIGARSVLHDCSIR 190

Query: 127 YEQYPFDD 135
           YEQYPF D
Sbjct: 191 YEQYPFTD 197

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
GNK2_GINBI1.2e-1133.08Antifungal protein ginkbilobin-2 OS=Ginkgo biloba GN=GNK2 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KGA2_CUCSA3.1e-7099.25Uncharacterized protein OS=Cucumis sativus GN=Csa_6G409950 PE=4 SV=1[more]
M5W194_PRUPE2.9e-4470.40Uncharacterized protein (Fragment) OS=Prunus persica GN=PRUPE_ppa025382mg PE=4 S... [more]
A0A067JPK6_JATCU3.8e-4469.53Uncharacterized protein OS=Jatropha curcas GN=JCGZ_21945 PE=4 SV=1[more]
B9H872_POPTR1.2e-4260.90Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0005s22990g PE=4 SV=2[more]
A0A0D2P8H3_GOSRA6.1e-4263.28Uncharacterized protein OS=Gossypium raimondii GN=B456_007G136000 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT4G23140.27.5e-0733.06 cysteine-rich RLK (RECEPTOR-like protein kinase) 6[more]
AT5G48540.19.8e-0729.70 receptor-like protein kinase-related family protein[more]
AT4G05200.11.3e-0638.46 cysteine-rich RLK (RECEPTOR-like protein kinase) 25[more]
Match NameE-valueIdentityDescription
gi|700192685|gb|KGN47889.1|4.5e-7099.25hypothetical protein Csa_6G409950 [Cucumis sativus][more]
gi|645259245|ref|XP_008235269.1|3.2e-4470.08PREDICTED: antifungal protein ginkbilobin-2-like [Prunus mume][more]
gi|595791225|ref|XP_007199361.1|4.2e-4470.40hypothetical protein PRUPE_ppa025382mg, partial [Prunus persica][more]
gi|802792380|ref|XP_012092263.1|5.5e-4469.53PREDICTED: antifungal protein ginkbilobin-2-like [Jatropha curcas][more]
gi|643704410|gb|KDP21474.1|5.5e-4469.53hypothetical protein JCGZ_21945 [Jatropha curcas][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR002902GNK2
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
biological_process GO:0016310 phosphorylation
cellular_component GO:0005575 cellular_component
molecular_function GO:0003674 molecular_function
molecular_function GO:0016301 kinase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI06G21950.1CSPI06G21950.1mRNA


Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002902Gnk2-homologous domainPFAMPF01657Stress-antifungcoord: 35..130
score: 3.6
IPR002902Gnk2-homologous domainPROFILEPS51473GNK2coord: 28..132
score: 16
NoneNo IPR availablePANTHERPTHR32080FAMILY NOT NAMEDcoord: 1..134
score: 1.0
NoneNo IPR availablePANTHERPTHR32080:SF10SUBFAMILY NOT NAMEDcoord: 1..134
score: 1.0