CSPI06G08660 (gene) Wild cucumber (PI 183967)

Overview
NameCSPI06G08660
Typegene
OrganismCucumis sativus (Wild cucumber (PI 183967))
DescriptionFlavanone-3-hydroxylase
LocationChr6 : 7284354 .. 7286050 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAACTCTGTTGTCTCTCTATGTATATATAGTAATCAGACCTTGAGAGGGAAACACTGATTTTTTCGTAGTACCAGTGTATAAGCCCCTCGCTCACTGTATAATTCTTTTTCATTCACCCCATTTCTCTGTATCTCATAATCCCGCATCGTCATGGCCACTTTTACTGACGATGAAATTCCGGTCATTTCCCTTGATGGAATCGAAGACGATGCCCACATGGCCGCGGTGAAGAAGAAAATCGTGGAGGCCTGCGAAGTGTGGGGAATATTCCAGGTGGTTGACCACGGTGTTGACCCCAAGCTTATTGCCGACATGGTTCGTCTCACCACCGAATTCTACTATTTTCCACCGGAAGAGAAGCTCCGATTCGACATGTCGGGCGGCAAGAGGGGCGGATTCATGATCTCTACTCGACGACAGGTCTCTCCAACTCCAACACTCTGTTTCACACTAACAACTACAATCCTCTGTTTTTCTTTTATCTTAATGAATTTTGTATTGAAAGAGTAAATGATAATAATGATTGGTGATAGAATTTATTCAGGGAGATGTAGAAGATTGGAGGGAAGTGGTAACGTTCTTTACATATCCGAGTAGAGATCGGAAGTACTCGGAATGGCCGGACAAACCGGAGGGTTGGATCAAGACGACGGAGGAGTTCAGTGAAAAGTTGATGGATTTGGCTTCCAAAATATTGGAAGTGTTGTCGGAAGCCATGGATATGGAGAAGGGAGCTCTTAAGAAAGCTTGTGGAGAGTTGAATCAAAAGATATTGACCAATTTTTACCCCAAATGTTCGAACACCGACCTCGAACTCGGCCTCCCTCGTCACACCGACCCTGGAACCATTACTATATTATTACAGGCAACCTCTCACTCTCTTTACATTTGTCTTACTTTTGTCTCTAAAGTTTCAAATTTCCATACCAAAGAATCCTTGGGATATTAATGAAAATTATATATACATACATATAATTAATTATAGGATCAAGTGGGTGGTCTTCAAGCAACCAAAGATGGGGCCAAGACATGGATCAACATCCCACCAATGGAAGGAGCTTTTGTAATCAACATTGGAGACCATGGTCATGTATGTACTACTCTCCATCTTAATTTCTGCAAAATGGATTATCTAACGGAATTGAAAACATGAGTAGAGATAATAGAAGTAATAGAAAAATTTTGTTCATTTTTAAAAACCAAACGATTAATATTAGAGAGAAGTCTAATAATTTATTATGTGATTGAAAAATGTAGTATCTTAGCAATGGGAGGTTCAGGAGTGCGGATCATAGGGCATTGGCGAACCCAAAGAGTGACAGATTGTCGATAGCTACGTTTCAGTATCCGACACCGGAAGCCATTGTTTATCCGTTGAAGGTTGGTGAAGGGGAAAAGGCAGTGATTGAGGATCCCATAAGCTTTGCAGAGATGTATAAGAAAAAGATGAGCAATGAACCTGTTCTTGCAATGTTTAATCATAAAGGACAAAACAAACTCAATCCTTCTACTTAGTTTATTGCTTTAGTTATAATTGTGTTGTAAGCTCAACTTTTTAATTTAGTTATATATTTCCCTAATATTTATGACTTTTTTAGTTTTCAATTTTGTTCTGTCCTGTTATGATCTTCAAAGCTTTCATTTTCATCTCTTTTGTGTACTTTTAATTTATTTCAATAAAGTATATTTATTGTTA

mRNA sequence

ATGGCCACTTTTACTGACGATGAAATTCCGGTCATTTCCCTTGATGGAATCGAAGACGATGCCCACATGGCCGCGGTGAAGAAGAAAATCGTGGAGGCCTGCGAAGTGTGGGGAATATTCCAGGTGGTTGACCACGGTGTTGACCCCAAGCTTATTGCCGACATGGTTCGTCTCACCACCGAATTCTACTATTTTCCACCGGAAGAGAAGCTCCGATTCGACATGTCGGGCGGCAAGAGGGGCGGATTCATGATCTCTACTCGACGACAGGGAGATGTAGAAGATTGGAGGGAAGTGGTAACGTTCTTTACATATCCGAGTAGAGATCGGAAGTACTCGGAATGGCCGGACAAACCGGAGGGTTGGATCAAGACGACGGAGGAGTTCAGTGAAAAGTTGATGGATTTGGCTTCCAAAATATTGGAAGTGTTGTCGGAAGCCATGGATATGGAGAAGGGAGCTCTTAAGAAAGCTTGTGGAGAGTTGAATCAAAAGATATTGACCAATTTTTACCCCAAATGTTCGAACACCGACCTCGAACTCGGCCTCCCTCGTCACACCGACCCTGGAACCATTACTATATTATTACAGGATCAAGTGGGTGGTCTTCAAGCAACCAAAGATGGGGCCAAGACATGGATCAACATCCCACCAATGGAAGGAGCTTTTGTAATCAACATTGGAGACCATGGTCATTATCTTAGCAATGGGAGGTTCAGGAGTGCGGATCATAGGGCATTGGCGAACCCAAAGAGTGACAGATTGTCGATAGCTACGTTTCAGTATCCGACACCGGAAGCCATTGTTTATCCGTTGAAGGTTGGTGAAGGGGAAAAGGCAGTGATTGAGGATCCCATAAGCTTTGCAGAGATGTATAAGAAAAAGATGAGCAATGAACCTGTTCTTGCAATGTTTAATCATAAAGGACAAAACAAACTCAATCCTTCTACTTAG

Coding sequence (CDS)

ATGGCCACTTTTACTGACGATGAAATTCCGGTCATTTCCCTTGATGGAATCGAAGACGATGCCCACATGGCCGCGGTGAAGAAGAAAATCGTGGAGGCCTGCGAAGTGTGGGGAATATTCCAGGTGGTTGACCACGGTGTTGACCCCAAGCTTATTGCCGACATGGTTCGTCTCACCACCGAATTCTACTATTTTCCACCGGAAGAGAAGCTCCGATTCGACATGTCGGGCGGCAAGAGGGGCGGATTCATGATCTCTACTCGACGACAGGGAGATGTAGAAGATTGGAGGGAAGTGGTAACGTTCTTTACATATCCGAGTAGAGATCGGAAGTACTCGGAATGGCCGGACAAACCGGAGGGTTGGATCAAGACGACGGAGGAGTTCAGTGAAAAGTTGATGGATTTGGCTTCCAAAATATTGGAAGTGTTGTCGGAAGCCATGGATATGGAGAAGGGAGCTCTTAAGAAAGCTTGTGGAGAGTTGAATCAAAAGATATTGACCAATTTTTACCCCAAATGTTCGAACACCGACCTCGAACTCGGCCTCCCTCGTCACACCGACCCTGGAACCATTACTATATTATTACAGGATCAAGTGGGTGGTCTTCAAGCAACCAAAGATGGGGCCAAGACATGGATCAACATCCCACCAATGGAAGGAGCTTTTGTAATCAACATTGGAGACCATGGTCATTATCTTAGCAATGGGAGGTTCAGGAGTGCGGATCATAGGGCATTGGCGAACCCAAAGAGTGACAGATTGTCGATAGCTACGTTTCAGTATCCGACACCGGAAGCCATTGTTTATCCGTTGAAGGTTGGTGAAGGGGAAAAGGCAGTGATTGAGGATCCCATAAGCTTTGCAGAGATGTATAAGAAAAAGATGAGCAATGAACCTGTTCTTGCAATGTTTAATCATAAAGGACAAAACAAACTCAATCCTTCTACTTAG
Homology
[Close]
BLAST of CSPI06G08660 vs. Swiss-Prot
Match: FL3H_ARATH (Naringenin,2-oxoglutarate 3-dioxygenase OS=Arabidopsis thaliana GN=F3H PE=1 SV=1)

HSP 1 Score: 432.2 bits (1110), Expect = 5.0e-120
Identity = 200/299 (66.89%), Postives = 247/299 (82.61%), Query Frame = 1 

Query: 7   DEIPVISLDGIED-DAHMAAVKKKIVEACEVWGIFQVVDHGVDPKLIADMVRLTTEFYYF 66
           DEIPVISL GI+D D     + ++IVEACE WGIFQVVDHGVD  L+ADM RL  +F+  
Sbjct: 36  DEIPVISLAGIDDVDGKRGEICRQIVEACENWGIFQVVDHGVDTNLVADMTRLARDFFAL 95

Query: 67  PPEEKLRFDMSGGKRGGFMISTRRQGD-VEDWREVVTFFTYPSRDRKYSEWPDKPEGWIK 126
           PPE+KLRFDMSGGK+GGF++S+  QG+ V+DWRE+VT+F+YP R+R YS WPDKPEGW+K
Sbjct: 96  PPEDKLRFDMSGGKKGGFIVSSHLQGEAVQDWREIVTYFSYPVRNRDYSRWPDKPEGWVK 155

Query: 127 TTEEFSEKLMDLASKILEVLSEAMDMEKGALKKACGELNQKILTNFYPKCSNTDLELGLP 186
            TEE+SE+LM LA K+LEVLSEAM +EK +L  AC +++QKI+ N+YPKC   DL LGL 
Sbjct: 156 VTEEYSERLMSLACKLLEVLSEAMGLEKESLTNACVDMDQKIVVNYYPKCPQPDLTLGLK 215

Query: 187 RHTDPGTITILLQDQVGGLQATKDGAKTWINIPPMEGAFVINIGDHGHYLSNGRFRSADH 246
           RHTDPGTIT+LLQDQVGGLQAT+D  KTWI + P+EGAFV+N+GDHGH+LSNGRF++ADH
Sbjct: 216 RHTDPGTITLLLQDQVGGLQATRDNGKTWITVQPVEGAFVVNLGDHGHFLSNGRFKNADH 275

Query: 247 RALANPKSDRLSIATFQYPTPEAIVYPLKVGEGEKAVIEDPISFAEMYKKKMSNEPVLA 304
           +A+ N  S RLSIATFQ P P+A VYPLKV EGEKA++E+PI+FAEMYK+KM  +  LA
Sbjct: 276 QAVVNSNSSRLSIATFQNPAPDATVYPLKVREGEKAILEEPITFAEMYKRKMGRDLELA 334

[Close]
BLAST of CSPI06G08660 vs. Swiss-Prot
Match: FL3H_MATIN (Naringenin,2-oxoglutarate 3-dioxygenase (Fragment) OS=Matthiola incana GN=FHT PE=2 SV=1)

HSP 1 Score: 429.9 bits (1104), Expect = 2.5e-119
Identity = 202/310 (65.16%), Postives = 248/310 (80.00%), Query Frame = 1 

Query: 7   DEIPVISLDGIED-DAHMAAVKKKIVEACEVWGIFQVVDHGVDPKLIADMVRLTTEFYYF 66
           DEIPVISL GI+D D     + ++IVEACE WGIFQVVDHGVD  L+ADM RL  +F+  
Sbjct: 35  DEIPVISLAGIDDVDGKRGEICREIVEACENWGIFQVVDHGVDTSLVADMTRLARDFFAL 94

Query: 67  PPEEKLRFDMSGGKRGGFMISTRRQGD-VEDWREVVTFFTYPSRDRKYSEWPDKPEGWIK 126
           PPEEKLRFDMSGGK+GGF++S+  QG+ V+DWRE+VT+F+YP R+R YS WPDKP+GW K
Sbjct: 95  PPEEKLRFDMSGGKKGGFIVSSHLQGEAVQDWREIVTYFSYPVRNRDYSRWPDKPQGWAK 154

Query: 127 TTEEFSEKLMDLASKILEVLSEAMDMEKGALKKACGELNQKILTNFYPKCSNTDLELGLP 186
            TEE+SEKLM LA K+LEVLSEAM +EK +L  AC +++QKI+ N+YPKC   DL LGL 
Sbjct: 155 VTEEYSEKLMGLACKLLEVLSEAMGLEKESLTNACVDMDQKIVVNYYPKCPQPDLTLGLK 214

Query: 187 RHTDPGTITILLQDQVGGLQATKDGAKTWINIPPMEGAFVINIGDHGHYLSNGRFRSADH 246
           RHTDPGTIT+LLQDQVGGLQAT+D   TWI + P+EGAFV+N+GDHGH+LSNGRF++ADH
Sbjct: 215 RHTDPGTITLLLQDQVGGLQATRDDGNTWITVQPVEGAFVVNLGDHGHFLSNGRFKNADH 274

Query: 247 RALANPKSDRLSIATFQYPTPEAIVYPLKVGEGEKAVIEDPISFAEMYKKKMSNEPVLAM 306
           +A+ N  S RLSIATFQ P PEA VYPLKV EGEKA++E+PI+FAEMYK+KM  +  LA 
Sbjct: 275 QAVVNSNSSRLSIATFQNPAPEATVYPLKVREGEKAIMEEPITFAEMYKRKMGRDLELAR 334

Query: 307 FNHKGQNKLN 315
                + + N
Sbjct: 335 LKKLAKEEHN 344

[Close]
BLAST of CSPI06G08660 vs. Swiss-Prot
Match: FL3H_PETHY (Naringenin,2-oxoglutarate 3-dioxygenase (Fragment) OS=Petunia hybrida GN=AN3 PE=1 SV=1)

HSP 1 Score: 426.0 bits (1094), Expect = 3.6e-118
Identity = 195/308 (63.31%), Postives = 252/308 (81.82%), Query Frame = 1 

Query: 7   DEIPVISLDGIEDDA-HMAAVKKKIVEACEVWGIFQVVDHGVDPKLIADMVRLTTEFYYF 66
           +EIP+ISL+GI+D+    A +  KIV+ACE WG+FQVVDHGVD ++I+ M     EF+  
Sbjct: 39  NEIPIISLEGIDDETGKRAEICDKIVKACEDWGVFQVVDHGVDAEVISQMTTFAKEFFAL 98

Query: 67  PPEEKLRFDMSGGKRGGFMISTRRQGDV-EDWREVVTFFTYPSRDRKYSEWPDKPEGWIK 126
           PPEEKLRFDMSGGK+GGF++S+  QG+V +DWRE+VT+F+YP+R R YS WPDKPEGWI 
Sbjct: 99  PPEEKLRFDMSGGKKGGFIVSSHLQGEVVQDWREIVTYFSYPTRARDYSRWPDKPEGWIA 158

Query: 127 TTEEFSEKLMDLASKILEVLSEAMDMEKGALKKACGELNQKILTNFYPKCSNTDLELGLP 186
            T+++SEKLM+LA K+L+VLSEAM +EK AL KAC +++QK++ NFYPKC   DL LGL 
Sbjct: 159 VTQKYSEKLMELACKLLDVLSEAMGLEKEALTKACVDMDQKVVVNFYPKCPEPDLTLGLK 218

Query: 187 RHTDPGTITILLQDQVGGLQATKDGAKTWINIPPMEGAFVINIGDHGHYLSNGRFRSADH 246
           RHTDPGTIT+LLQDQVGGLQATKD  KTWI + P+EGAFV+N+GDHGH+LSNGRF++ADH
Sbjct: 219 RHTDPGTITLLLQDQVGGLQATKDNGKTWITVQPVEGAFVVNLGDHGHFLSNGRFKNADH 278

Query: 247 RALANPKSDRLSIATFQYPTPEAIVYPLKVGEGEKAVIEDPISFAEMYKKKMSNEPVLAM 306
           +A+ N  S RLSIATFQ P PEAIVYPLK+ EGEK+++++PI+FAEMY++KMS +  LA 
Sbjct: 279 QAVVNSNSSRLSIATFQNPAPEAIVYPLKIREGEKSIMDEPITFAEMYRRKMSKDLELAR 338

Query: 307 FNHKGQNK 313
              + + +
Sbjct: 339 LKKQAKEQ 346

[Close]
BLAST of CSPI06G08660 vs. Swiss-Prot
Match: FL3H_PETCR (Flavanone 3-dioxygenase OS=Petroselinum crispum GN=FHT PE=1 SV=1)

HSP 1 Score: 417.9 bits (1073), Expect = 9.7e-116
Identity = 195/310 (62.90%), Postives = 248/310 (80.00%), Query Frame = 1 

Query: 7   DEIPVISLDGIEDDA--HMAAVKKKIVEACEVWGIFQVVDHGVDPKLIADMVRLTTEFYY 66
           DEIPVISL GI+DD+    + + +KIVEACE WGIFQVVDHG+D  LI++M RL  +F+ 
Sbjct: 36  DEIPVISLAGIDDDSVDKRSQICRKIVEACEDWGIFQVVDHGIDIDLISEMTRLARQFFA 95

Query: 67  FPPEEKLRFDMSGGKRGGFMISTRRQGD-VEDWREVVTFFTYPSRDRKYSEWPDKPEGWI 126
            P EEKLRFDM+GGK+GGF++S+  QG+ V+DWRE+VT+F+YP + R YS WPDKPEGW 
Sbjct: 96  LPAEEKLRFDMTGGKKGGFIVSSHLQGEAVQDWREIVTYFSYPIQARDYSRWPDKPEGWR 155

Query: 127 KTTEEFSEKLMDLASKILEVLSEAMDMEKGALKKACGELNQKILTNFYPKCSNTDLELGL 186
             TE +S++LM LA K+LEVLSEAM +EK  L KAC +++QK++ N+YPKC   +L LGL
Sbjct: 156 SITEMYSDELMALACKLLEVLSEAMGLEKEGLTKACVDMDQKVIVNYYPKCPQPNLTLGL 215

Query: 187 PRHTDPGTITILLQDQVGGLQATKDGAKTWINIPPMEGAFVINIGDHGHYLSNGRFRSAD 246
            RHTDPGTIT+LLQDQVGGLQAT+DG KTWI + P+EGAFV+N+GDHGHYLSNGRF++AD
Sbjct: 216 KRHTDPGTITLLLQDQVGGLQATRDGGKTWITVQPVEGAFVVNLGDHGHYLSNGRFKNAD 275

Query: 247 HRALANPKSDRLSIATFQYPTPEAIVYPLKVGEGEKAVIEDPISFAEMYKKKMSNEPVLA 306
           H+A+ N  S R+SIATFQ P P A VYPLK+ EGEKAV+E+PI+FAEMYK+KMS +  +A
Sbjct: 276 HQAVVNSNSSRMSIATFQNPAPNATVYPLKIREGEKAVMEEPITFAEMYKRKMSRDIEMA 335

Query: 307 MFNHKGQNKL 314
                 + K+
Sbjct: 336 TLKKLAKEKV 345

[Close]
BLAST of CSPI06G08660 vs. Swiss-Prot
Match: FL3H_VITVI (Naringenin,2-oxoglutarate 3-dioxygenase OS=Vitis vinifera GN=F3H PE=2 SV=1)

HSP 1 Score: 417.2 bits (1071), Expect = 1.7e-115
Identity = 198/302 (65.56%), Postives = 244/302 (80.79%), Query Frame = 1 

Query: 7   DEIPVISLDGIEDDAHMAAVK----KKIVEACEVWGIFQVVDHGVDPKLIADMVRLTTEF 66
           +EIPVISL   ++   +AAV     +KIVEACE WGIFQVV+HGVD  LI++M RL  EF
Sbjct: 36  NEIPVISLT--KESMKLAAVVDEICRKIVEACEDWGIFQVVNHGVDSNLISEMTRLAREF 95

Query: 67  YYFPPEEKLRFDMSGGKRGGFMISTRRQGD-VEDWREVVTFFTYPSRDRKYSEWPDKPEG 126
           +  PPEE +RFDMSGGK+GGF++S+  QG+ V+DWRE+VT+F+YP R R YS WPDKPEG
Sbjct: 96  FALPPEENVRFDMSGGKKGGFIVSSHLQGEAVQDWREIVTYFSYPLRTRDYSRWPDKPEG 155

Query: 127 WIKTTEEFSEKLMDLASKILEVLSEAMDMEKGALKKACGELNQKILTNFYPKCSNTDLEL 186
           W   T+E+SEKLM LA K+LEVLSEAMD++K AL  AC +++QK++ NFYP+C   DL L
Sbjct: 156 WRSVTQEYSEKLMGLACKLLEVLSEAMDLDKDALTNACVDMDQKVVVNFYPQCPQPDLTL 215

Query: 187 GLPRHTDPGTITILLQDQVGGLQATKDGAKTWINIPPMEGAFVINIGDHGHYLSNGRFRS 246
           GL RHTDPGTIT+LLQDQVGGLQAT+DG KTWI + P+EGAFV+N+GDHGHYLSNGRF++
Sbjct: 216 GLKRHTDPGTITLLLQDQVGGLQATRDGGKTWITVQPVEGAFVVNLGDHGHYLSNGRFKN 275

Query: 247 ADHRALANPKSDRLSIATFQYPTPEAIVYPLKVGEGEKAVIEDPISFAEMYKKKMSNEPV 304
           ADH+A+ N    RLSIATFQ P PEA VYPLK+ EGEKAV+E PI+FAEMY++KMS +  
Sbjct: 276 ADHQAVVNSNHSRLSIATFQNPAPEATVYPLKIREGEKAVLEGPITFAEMYRRKMSKDLE 335

[Close]
BLAST of CSPI06G08660 vs. TrEMBL
Match: A0A0A0KAQ6_CUCSA (Flavanone-3-hydroxylase OS=Cucumis sativus GN=Csa_6G108510 PE=3 SV=1)

HSP 1 Score: 652.1 bits (1681), Expect = 3.4e-184
Identity = 316/317 (99.68%), Postives = 316/317 (99.68%), Query Frame = 1 

Query: 1   MATFTDDEIPVISLDGIEDDAHMAAVKKKIVEACEVWGIFQVVDHGVDPKLIADMVRLTT 60
           MATFTDDEIPVISLDGIEDDAHMAAVKKKIVEACEVWGIFQVVDHGVDPKLIADMVRLTT
Sbjct: 1   MATFTDDEIPVISLDGIEDDAHMAAVKKKIVEACEVWGIFQVVDHGVDPKLIADMVRLTT 60

Query: 61  EFYYFPPEEKLRFDMSGGKRGGFMISTRRQGDVEDWREVVTFFTYPSRDRKYSEWPDKPE 120
           EFYYFPPEEKLRFDMSGGKRGGFMISTRRQGDVEDWREVVTFFTYPSRDRKYSEWPDKPE
Sbjct: 61  EFYYFPPEEKLRFDMSGGKRGGFMISTRRQGDVEDWREVVTFFTYPSRDRKYSEWPDKPE 120

Query: 121 GWIKTTEEFSEKLMDLASKILEVLSEAMDMEKGALKKACGELNQKILTNFYPKCSNTDLE 180
           GWIK TEEFSEKLMDLASKILEVLSEAMDMEKGALKKACGELNQKILTNFYPKCSNTDLE
Sbjct: 121 GWIKKTEEFSEKLMDLASKILEVLSEAMDMEKGALKKACGELNQKILTNFYPKCSNTDLE 180

Query: 181 LGLPRHTDPGTITILLQDQVGGLQATKDGAKTWINIPPMEGAFVINIGDHGHYLSNGRFR 240
           LGLPRHTDPGTITILLQDQVGGLQATKDGAKTWINIPPMEGAFVINIGDHGHYLSNGRFR
Sbjct: 181 LGLPRHTDPGTITILLQDQVGGLQATKDGAKTWINIPPMEGAFVINIGDHGHYLSNGRFR 240

Query: 241 SADHRALANPKSDRLSIATFQYPTPEAIVYPLKVGEGEKAVIEDPISFAEMYKKKMSNEP 300
           SADHRALANPKSDRLSIATFQYPTPEAIVYPLKVGEGEKAVIEDPISFAEMYKKKMSNEP
Sbjct: 241 SADHRALANPKSDRLSIATFQYPTPEAIVYPLKVGEGEKAVIEDPISFAEMYKKKMSNEP 300

Query: 301 VLAMFNHKGQNKLNPST 318
           VLAMFNHKGQNKLNPST
Sbjct: 301 VLAMFNHKGQNKLNPST 317

[Close]
BLAST of CSPI06G08660 vs. TrEMBL
Match: A0A142EGS0_9SOLA (Flavanone 3-hydroxylase OS=Iochroma grandiflorum PE=2 SV=1)

HSP 1 Score: 436.4 bits (1121), Expect = 2.9e-119
Identity = 200/298 (67.11%), Postives = 248/298 (83.22%), Query Frame = 1 

Query: 7   DEIPVISLDGIEDDAHMAAVKKKIVEACEVWGIFQVVDHGVDPKLIADMVRLTTEFYYFP 66
           DEIPVISL G+++      + +KIV+ACE WG+FQVVDHGVD +LI+ M +   EF+  P
Sbjct: 36  DEIPVISLKGLDESGKRGEICEKIVQACEDWGVFQVVDHGVDDELISQMTKFAKEFFALP 95

Query: 67  PEEKLRFDMSGGKRGGFMISTRRQGDV-EDWREVVTFFTYPSRDRKYSEWPDKPEGWIKT 126
           PEEKLRFDMSGGK+GGF++S+  QG+V +DWRE+VT+F+YP+R R YS WPDKP+GWI  
Sbjct: 96  PEEKLRFDMSGGKKGGFIVSSHLQGEVVQDWREIVTYFSYPTRARDYSRWPDKPQGWIAV 155

Query: 127 TEEFSEKLMDLASKILEVLSEAMDMEKGALKKACGELNQKILTNFYPKCSNTDLELGLPR 186
           TE++SEKLMDLA K+LEVLSEAM +EK AL KAC +++QK++ NFYPKC   DL LGL R
Sbjct: 156 TEKYSEKLMDLACKLLEVLSEAMGLEKEALTKACVDMDQKVVVNFYPKCPQPDLTLGLKR 215

Query: 187 HTDPGTITILLQDQVGGLQATKDGAKTWINIPPMEGAFVINIGDHGHYLSNGRFRSADHR 246
           HTDPGTIT+LLQDQVGGLQATKD  KTWI + P+EGAFV+N+GDHGHYLSNGRF++ADH+
Sbjct: 216 HTDPGTITLLLQDQVGGLQATKDNGKTWITVQPVEGAFVVNLGDHGHYLSNGRFKNADHQ 275

Query: 247 ALANPKSDRLSIATFQYPTPEAIVYPLKVGEGEKAVIEDPISFAEMYKKKMSNEPVLA 304
           A+ N  S RLSIATFQ P PEAIVYPLK+ EGEKAV+++PI+FAEMY++KMS +  LA
Sbjct: 276 AVVNSNSSRLSIATFQNPAPEAIVYPLKIREGEKAVMDEPITFAEMYRRKMSKDLELA 333

[Close]
BLAST of CSPI06G08660 vs. TrEMBL
Match: A0A142EGR1_9SOLA (Flavanone 3-hydroxylase OS=Iochroma umbellatum PE=2 SV=1)

HSP 1 Score: 435.3 bits (1118), Expect = 6.6e-119
Identity = 200/298 (67.11%), Postives = 247/298 (82.89%), Query Frame = 1 

Query: 7   DEIPVISLDGIEDDAHMAAVKKKIVEACEVWGIFQVVDHGVDPKLIADMVRLTTEFYYFP 66
           DEIPVISL G+++      + +KIV+ACE WG+FQVVDHGVD +LI+ M     EF+  P
Sbjct: 36  DEIPVISLKGLDESGKRGEICEKIVKACEDWGVFQVVDHGVDAELISQMTNFAKEFFALP 95

Query: 67  PEEKLRFDMSGGKRGGFMISTRRQGDV-EDWREVVTFFTYPSRDRKYSEWPDKPEGWIKT 126
           PEEKLRFDMSGGK+GGF++S+  QG+V +DWRE+VT+F+YP+R R YS WPDKP+GWI  
Sbjct: 96  PEEKLRFDMSGGKKGGFIVSSHLQGEVVQDWREIVTYFSYPTRARDYSRWPDKPQGWIAV 155

Query: 127 TEEFSEKLMDLASKILEVLSEAMDMEKGALKKACGELNQKILTNFYPKCSNTDLELGLPR 186
           TE++SEKLMDLA K+LEVLSEAM +EK AL KAC +++QK++ NFYPKC   DL LGL R
Sbjct: 156 TEKYSEKLMDLACKLLEVLSEAMGLEKEALTKACVDMDQKVVVNFYPKCPQPDLTLGLKR 215

Query: 187 HTDPGTITILLQDQVGGLQATKDGAKTWINIPPMEGAFVINIGDHGHYLSNGRFRSADHR 246
           HTDPGTIT+LLQDQVGGLQATKD  KTWI + P+EGAFV+N+GDHGHYLSNGRF++ADH+
Sbjct: 216 HTDPGTITLLLQDQVGGLQATKDNGKTWITVQPVEGAFVVNLGDHGHYLSNGRFKNADHQ 275

Query: 247 ALANPKSDRLSIATFQYPTPEAIVYPLKVGEGEKAVIEDPISFAEMYKKKMSNEPVLA 304
           A+ N  S RLSIATFQ P PEAIVYPLK+ EGEKAV+++PI+FAEMY++KMS +  LA
Sbjct: 276 AVVNSNSSRLSIATFQNPAPEAIVYPLKIREGEKAVMDEPITFAEMYRRKMSKDLELA 333

[Close]
BLAST of CSPI06G08660 vs. TrEMBL
Match: F1CJI9_9ERIC (Flavanone 3-hydroxylase OS=Camellia nitidissima GN=F3H PE=2 SV=1)

HSP 1 Score: 433.0 bits (1112), Expect = 3.3e-118
Identity = 204/309 (66.02%), Postives = 249/309 (80.58%), Query Frame = 1 

Query: 7   DEIPVISLDGIED-DAHMAAVKKKIVEACEVWGIFQVVDHGVDPKLIADMVRLTTEFYYF 66
           +EIPVISL GI++ +   + + +KIVEACE WG+FQVVDHGVD  LIA+M RL  EF+  
Sbjct: 37  NEIPVISLAGIDEIEGRRSEICRKIVEACEDWGVFQVVDHGVDANLIAEMTRLAREFFAL 96

Query: 67  PPEEKLRFDMSGGKRGGFMISTRRQGD-VEDWREVVTFFTYPSRDRKYSEWPDKPEGWIK 126
           PPEEKLRFDMSGGK+GGF++S+  QG+ V+DWRE+VT+F+YP R R YS WPDKPEGW  
Sbjct: 97  PPEEKLRFDMSGGKKGGFIVSSHLQGEAVQDWREIVTYFSYPIRARDYSRWPDKPEGWSA 156

Query: 127 TTEEFSEKLMDLASKILEVLSEAMDMEKGALKKACGELNQKILTNFYPKCSNTDLELGLP 186
            TE +SEKLMDLA K+LEVLSEAM +EK AL KAC +++QK++ NFYPKC   DL LGL 
Sbjct: 157 VTETYSEKLMDLACKLLEVLSEAMGLEKEALTKACVDMDQKVVINFYPKCPQPDLTLGLK 216

Query: 187 RHTDPGTITILLQDQVGGLQATKDGAKTWINIPPMEGAFVINIGDHGHYLSNGRFRSADH 246
           RHTDPGTIT+LLQDQVGGLQAT+DG KTWI + P+EGAFV+N+GDHGHYLSNGRF++ADH
Sbjct: 217 RHTDPGTITLLLQDQVGGLQATRDGGKTWITVQPVEGAFVVNLGDHGHYLSNGRFKNADH 276

Query: 247 RALANPKSDRLSIATFQYPTPEAIVYPLKVGEGEKAVIEDPISFAEMYKKKMSNEPVLAM 306
           +A+ N    RLSIATFQ P PEA VYPLK+ EGEK V+E+PI+FA+MYK+KMS +  LA 
Sbjct: 277 QAVVNSNCSRLSIATFQNPAPEATVYPLKIREGEKPVLEEPITFADMYKRKMSKDIELAK 336

Query: 307 FNHKGQNKL 314
                + KL
Sbjct: 337 LKKLAKEKL 345

[Close]
BLAST of CSPI06G08660 vs. TrEMBL
Match: A0A142EGQ7_9SOLA (Flavanone 3-hydroxylase OS=Iochroma tupayachianum PE=2 SV=1)

HSP 1 Score: 433.0 bits (1112), Expect = 3.3e-118
Identity = 200/298 (67.11%), Postives = 247/298 (82.89%), Query Frame = 1 

Query: 7   DEIPVISLDGIEDDAHMAAVKKKIVEACEVWGIFQVVDHGVDPKLIADMVRLTTEFYYFP 66
           DEIPVISL GI+++     + +KIV+ACE WG+FQVVDHGVD +LI+ M +   EF+  P
Sbjct: 36  DEIPVISLKGIDENEKRGEICEKIVKACEDWGVFQVVDHGVDDELISQMTKFAKEFFALP 95

Query: 67  PEEKLRFDMSGGKRGGFMISTRRQGDV-EDWREVVTFFTYPSRDRKYSEWPDKPEGWIKT 126
           PEEKLRFDMSGGK+GGF++S+  QG+V +DWRE+VT+F+YP   R YS WPDKP+GWI  
Sbjct: 96  PEEKLRFDMSGGKKGGFIVSSHLQGEVVQDWREIVTYFSYPIGARDYSRWPDKPQGWIAV 155

Query: 127 TEEFSEKLMDLASKILEVLSEAMDMEKGALKKACGELNQKILTNFYPKCSNTDLELGLPR 186
           TE++SEKLMDLA K+LEVLSEAM +EK AL KAC +++QK++ NFYPKC   DL LGL R
Sbjct: 156 TEKYSEKLMDLACKLLEVLSEAMGLEKEALTKACVDMDQKVVVNFYPKCPQPDLTLGLKR 215

Query: 187 HTDPGTITILLQDQVGGLQATKDGAKTWINIPPMEGAFVINIGDHGHYLSNGRFRSADHR 246
           HTDPGTIT+LLQDQVGGLQATKD  KTWI + P+EGAFV+N+GDHGHYLSNGRF++ADH+
Sbjct: 216 HTDPGTITLLLQDQVGGLQATKDNGKTWITVQPVEGAFVVNLGDHGHYLSNGRFKNADHQ 275

Query: 247 ALANPKSDRLSIATFQYPTPEAIVYPLKVGEGEKAVIEDPISFAEMYKKKMSNEPVLA 304
           A+ N  S RLSIATFQ P PEAIVYPLK+ EGEKAV+++PI+FAEMY++KMS +  LA
Sbjct: 276 AVVNSNSSRLSIATFQNPAPEAIVYPLKIREGEKAVMDEPITFAEMYRRKMSKDLELA 333

[Close]
BLAST of CSPI06G08660 vs. TAIR10
Match: AT3G51240.1 (AT3G51240.1 flavanone 3-hydroxylase)

HSP 1 Score: 432.2 bits (1110), Expect = 2.8e-121
Identity = 200/299 (66.89%), Postives = 247/299 (82.61%), Query Frame = 1 

Query: 7   DEIPVISLDGIED-DAHMAAVKKKIVEACEVWGIFQVVDHGVDPKLIADMVRLTTEFYYF 66
           DEIPVISL GI+D D     + ++IVEACE WGIFQVVDHGVD  L+ADM RL  +F+  
Sbjct: 36  DEIPVISLAGIDDVDGKRGEICRQIVEACENWGIFQVVDHGVDTNLVADMTRLARDFFAL 95

Query: 67  PPEEKLRFDMSGGKRGGFMISTRRQGD-VEDWREVVTFFTYPSRDRKYSEWPDKPEGWIK 126
           PPE+KLRFDMSGGK+GGF++S+  QG+ V+DWRE+VT+F+YP R+R YS WPDKPEGW+K
Sbjct: 96  PPEDKLRFDMSGGKKGGFIVSSHLQGEAVQDWREIVTYFSYPVRNRDYSRWPDKPEGWVK 155

Query: 127 TTEEFSEKLMDLASKILEVLSEAMDMEKGALKKACGELNQKILTNFYPKCSNTDLELGLP 186
            TEE+SE+LM LA K+LEVLSEAM +EK +L  AC +++QKI+ N+YPKC   DL LGL 
Sbjct: 156 VTEEYSERLMSLACKLLEVLSEAMGLEKESLTNACVDMDQKIVVNYYPKCPQPDLTLGLK 215

Query: 187 RHTDPGTITILLQDQVGGLQATKDGAKTWINIPPMEGAFVINIGDHGHYLSNGRFRSADH 246
           RHTDPGTIT+LLQDQVGGLQAT+D  KTWI + P+EGAFV+N+GDHGH+LSNGRF++ADH
Sbjct: 216 RHTDPGTITLLLQDQVGGLQATRDNGKTWITVQPVEGAFVVNLGDHGHFLSNGRFKNADH 275

Query: 247 RALANPKSDRLSIATFQYPTPEAIVYPLKVGEGEKAVIEDPISFAEMYKKKMSNEPVLA 304
           +A+ N  S RLSIATFQ P P+A VYPLKV EGEKA++E+PI+FAEMYK+KM  +  LA
Sbjct: 276 QAVVNSNSSRLSIATFQNPAPDATVYPLKVREGEKAILEEPITFAEMYKRKMGRDLELA 334

[Close]
BLAST of CSPI06G08660 vs. TAIR10
Match: AT5G24530.1 (AT5G24530.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 180.6 bits (457), Expect = 1.5e-45
Identity = 103/296 (34.80%), Postives = 166/296 (56.08%), Query Frame = 1 

Query: 7   DEIPVISLDGIEDDAHMAAVKKKIVEACEVWGIFQVVDHGVDPKLIADMVRLTTEFYYFP 66
           ++ P+I L   +     + + ++I +AC  +G FQV++HGV+ ++I +MV +  EF+   
Sbjct: 36  EDFPLIDLSSTD----RSFLIQQIHQACARFGFFQVINHGVNKQIIDEMVSVAREFFSMS 95

Query: 67  PEEKLRF---DMSGGKRGGFMISTRRQGDVEDWREVVTFFTYPSRDRKYSEWPDKPEGWI 126
            EEK++    D +   R     + +++ +V +WR+ +    YP   +  +EWP  P  + 
Sbjct: 96  MEEKMKLYSDDPTKTTRLSTSFNVKKE-EVNNWRDYLRLHCYPIH-KYVNEWPSNPPSFK 155

Query: 127 KTTEEFSEKLMDLASKILEVLSEAMDMEKGALKKACGELNQKILTNFYPKCSNTDLELGL 186
           +   ++S ++ ++  KI E++SE++ +EK  +KK  GE  Q +  N+YP C   +L  GL
Sbjct: 156 EIVSKYSREVREVGFKIEELISESLGLEKDYMKKVLGEQGQHMAVNYYPPCPEPELTYGL 215

Query: 187 PRHTDPGTITILLQD-QVGGLQATKDGAKTWINIPPMEGAFVINIGDHGHYLSNGRFRSA 246
           P HTDP  +TILLQD  V GLQ   DG   W  + P   AFVINIGD    LSNG ++S 
Sbjct: 216 PAHTDPNALTILLQDTTVCGLQILIDG--QWFAVNPHPDAFVINIGDQLQALSNGVYKSV 275

Query: 247 DHRALANPKSDRLSIATFQYPTPEAIVYPLK----VGEGEKAVIEDPISFAEMYKK 295
            HRA+ N ++ RLS+A+F  P   A++ P K      + E   +    ++AE YKK
Sbjct: 276 WHRAVTNTENPRLSVASFLCPADCAVMSPAKPLWEAEDDETKPVYKDFTYAEYYKK 323

[Close]
BLAST of CSPI06G08660 vs. TAIR10
Match: AT1G55290.1 (AT1G55290.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 174.5 bits (441), Expect = 1.1e-43
Identity = 98/287 (34.15%), Postives = 159/287 (55.40%), Query Frame = 1 

Query: 5   TDDEIPVISLDGIEDDAHMAAVKKKIVEACEVWGIFQVVDHGVDPKLIADMVRLTTEFYY 64
           +D+ IPVI +  +++ +    V K + +A E WG FQV++HGV  +++ +M   T  F+ 
Sbjct: 58  SDESIPVIDISNLDEKS----VSKAVCDAAEEWGFFQVINHGVSMEVLENMKTATHRFFG 117

Query: 65  FPPEEKLRFD----MSGGKRGGFMISTRRQGDVEDWREVVTFFTYPSRDRKYSEWPDKPE 124
            P EEK +F     +S   R G   S   +  +E W++ ++ F + S       WPD   
Sbjct: 118 LPVEEKRKFSREKSLSTNVRFGTSFSPHAEKALE-WKDYLSLF-FVSEAEASQLWPDSCR 177

Query: 125 GWIKTTEEFSEKLMDLASKILEVLSEAMDM-EKGALKKACGELNQKILTNFYPKCSNTDL 184
                T E+  +   L  K+L  L E +++ E    K++    + +I  N+YP C N +L
Sbjct: 178 S---ETLEYMNETKPLVKKLLRFLGENLNVKELDKTKESFFMGSTRINLNYYPICPNPEL 237

Query: 185 ELGLPRHTDPGTITILLQDQVGGLQATKDGAKTWINIPPMEGAFVINIGDHGHYLSNGRF 244
            +G+ RH+D  ++TILLQD++GGL         W+++PP+ G+ VINIGD    +SNGR+
Sbjct: 238 TVGVGRHSDVSSLTILLQDEIGGLHVRSLTTGRWVHVPPISGSLVINIGDAMQIMSNGRY 297

Query: 245 RSADHRALANPKSDRLSIATFQYPTPEAIVYPL--KVGEGEKAVIED 285
           +S +HR LAN   +R+S+  F  P PE+++ PL   +  GEK V +D
Sbjct: 298 KSVEHRVLANGSYNRISVPIFVSPKPESVIGPLLEVIENGEKPVYKD 335

[Close]
BLAST of CSPI06G08660 vs. TAIR10
Match: AT4G10500.1 (AT4G10500.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 174.1 bits (440), Expect = 1.4e-43
Identity = 92/292 (31.51%), Postives = 155/292 (53.08%), Query Frame = 1 

Query: 7   DEIPVISLDGIEDDAHMAAVKKKIVEACEVWGIFQVVDHGVDPKLIADMVRLTTEFYYFP 66
           D IP+I L  +    + A + +++  AC  +G FQ+ +HGV    +  M  +  EF++ P
Sbjct: 42  DSIPLIDLRDLHGP-NRAVIVQQLASACSTYGFFQIKNHGVPDTTVNKMQTVAREFFHQP 101

Query: 67  PEEKLRFDMSGGKRGGFMISTRRQG--DVEDWREVVTFFTYPSRDRKYSEWPDKPEGWIK 126
             E+++   +   +   + ++   G   V +WR+ +    +P  D    EWP  P  + +
Sbjct: 102 ESERVKHYSADPTKTTRLSTSFNVGADKVLNWRDFLRLHCFPIEDF-IEEWPSSPISFRE 161

Query: 127 TTEEFSEKLMDLASKILEVLSEAMDMEKGALKKACGELNQKILTNFYPKCSNTDLELGLP 186
            T E++  +  L  ++LE +SE++ +E   +    G+  Q +  N+YP C   +L  GLP
Sbjct: 162 VTAEYATSVRALVLRLLEAISESLGLESDHISNILGKHAQHMAFNYYPPCPEPELTYGLP 221

Query: 187 RHTDPGTITILLQDQVGGLQATKDGAKTWINIPPMEGAFVINIGDHGHYLSNGRFRSADH 246
            H DP  IT+LLQDQV GLQ  KD    W+ + P+   F++NIGD    +SN +++S  H
Sbjct: 222 GHKDPTVITVLLQDQVSGLQVFKD--DKWVAVSPIPNTFIVNIGDQMQVISNDKYKSVLH 281

Query: 247 RALANPKSDRLSIATFQYPTPEAIVYPLK--VGEGEKAVIEDPISFAEMYKK 295
           RA+ N +++RLSI TF +P+ +A++ P    V E +   I     F E + K
Sbjct: 282 RAVVNTENERLSIPTFYFPSTDAVIGPAHELVNEQDSLAIYRTYPFVEYWDK 329

[Close]
BLAST of CSPI06G08660 vs. TAIR10
Match: AT3G21420.1 (AT3G21420.1 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein)

HSP 1 Score: 172.6 bits (436), Expect = 4.0e-43
Identity = 90/270 (33.33%), Postives = 154/270 (57.04%), Query Frame = 1 

Query: 8   EIPVISLDGIE--DDAHMAAVKKKIVEACEVWGIFQVVDHGVDPKLIADMVRLTTEFYYF 67
           +IPVI L  +   D+        K+ +ACE WG FQV++HG++ +++ D+  + +EF+  
Sbjct: 54  QIPVIDLSKLSKPDNDDFFFEILKLSQACEDWGFFQVINHGIEVEVVEDIEEVASEFFDM 113

Query: 68  PPEEKLRFDMSGGKRGGFMISTRRQGDVE-DWREVVTFFTYPSRDRKYSEWPDKPEGWIK 127
           P EEK ++ M  G   G+  +     D + DW  +     +P + R    WP KP  + +
Sbjct: 114 PLEEKKKYPMEPGTVQGYGQAFIFSEDQKLDWCNMFALGVHPPQIRNPKLWPSKPARFSE 173

Query: 128 TTEEFSEKLMDLASKILEVLSEAMDMEKGALKKACGELNQKILTNFYPKCSNTDLELGLP 187
           + E +S+++ +L  ++L+ ++ ++ +++   ++  GE  Q +  N+YP CS+ DL LGL 
Sbjct: 174 SLEGYSKEIRELCKRLLKYIAISLGLKEERFEEMFGEAVQAVRMNYYPPCSSPDLVLGLS 233

Query: 188 RHTDPGTITILLQDQVG--GLQATKDGAKTWINIPPMEGAFVINIGDHGHYLSNGRFRSA 247
            H+D   +T+L Q +    GLQ  KD   TW+ + P+  A VINIGD    LSNG+++S 
Sbjct: 234 PHSDGSALTVLQQSKNSCVGLQILKD--NTWVPVKPLPNALVINIGDTIEVLSNGKYKSV 293

Query: 248 DHRALANPKSDRLSIATFQYPTPEAIVYPL 273
           +HRA+ N + +RL+I TF  P  E  + P+
Sbjct: 294 EHRAVTNREKERLTIVTFYAPNYEVEIEPM 321

[Close]
BLAST of CSPI06G08660 vs. NCBI nr
Match: gi|449445471|ref|XP_004140496.1| (PREDICTED: naringenin,2-oxoglutarate 3-dioxygenase-like [Cucumis sativus])

HSP 1 Score: 652.1 bits (1681), Expect = 4.9e-184
Identity = 316/317 (99.68%), Postives = 316/317 (99.68%), Query Frame = 1 

Query: 1   MATFTDDEIPVISLDGIEDDAHMAAVKKKIVEACEVWGIFQVVDHGVDPKLIADMVRLTT 60
           MATFTDDEIPVISLDGIEDDAHMAAVKKKIVEACEVWGIFQVVDHGVDPKLIADMVRLTT
Sbjct: 1   MATFTDDEIPVISLDGIEDDAHMAAVKKKIVEACEVWGIFQVVDHGVDPKLIADMVRLTT 60

Query: 61  EFYYFPPEEKLRFDMSGGKRGGFMISTRRQGDVEDWREVVTFFTYPSRDRKYSEWPDKPE 120
           EFYYFPPEEKLRFDMSGGKRGGFMISTRRQGDVEDWREVVTFFTYPSRDRKYSEWPDKPE
Sbjct: 61  EFYYFPPEEKLRFDMSGGKRGGFMISTRRQGDVEDWREVVTFFTYPSRDRKYSEWPDKPE 120

Query: 121 GWIKTTEEFSEKLMDLASKILEVLSEAMDMEKGALKKACGELNQKILTNFYPKCSNTDLE 180
           GWIK TEEFSEKLMDLASKILEVLSEAMDMEKGALKKACGELNQKILTNFYPKCSNTDLE
Sbjct: 121 GWIKKTEEFSEKLMDLASKILEVLSEAMDMEKGALKKACGELNQKILTNFYPKCSNTDLE 180

Query: 181 LGLPRHTDPGTITILLQDQVGGLQATKDGAKTWINIPPMEGAFVINIGDHGHYLSNGRFR 240
           LGLPRHTDPGTITILLQDQVGGLQATKDGAKTWINIPPMEGAFVINIGDHGHYLSNGRFR
Sbjct: 181 LGLPRHTDPGTITILLQDQVGGLQATKDGAKTWINIPPMEGAFVINIGDHGHYLSNGRFR 240

Query: 241 SADHRALANPKSDRLSIATFQYPTPEAIVYPLKVGEGEKAVIEDPISFAEMYKKKMSNEP 300
           SADHRALANPKSDRLSIATFQYPTPEAIVYPLKVGEGEKAVIEDPISFAEMYKKKMSNEP
Sbjct: 241 SADHRALANPKSDRLSIATFQYPTPEAIVYPLKVGEGEKAVIEDPISFAEMYKKKMSNEP 300

Query: 301 VLAMFNHKGQNKLNPST 318
           VLAMFNHKGQNKLNPST
Sbjct: 301 VLAMFNHKGQNKLNPST 317

[Close]
BLAST of CSPI06G08660 vs. NCBI nr
Match: gi|659119588|ref|XP_008459735.1| (PREDICTED: naringenin,2-oxoglutarate 3-dioxygenase-like [Cucumis melo])

HSP 1 Score: 622.1 bits (1603), Expect = 5.4e-175
Identity = 297/317 (93.69%), Postives = 311/317 (98.11%), Query Frame = 1 

Query: 1   MATFTDDEIPVISLDGIEDDAHMAAVKKKIVEACEVWGIFQVVDHGVDPKLIADMVRLTT 60
           MATFT+DEIPVISL GI+DDAHMAAVKKKIVEACEVWGIFQVVDHGVDPKLI DM+RLTT
Sbjct: 1   MATFTEDEIPVISLAGIDDDAHMAAVKKKIVEACEVWGIFQVVDHGVDPKLIDDMIRLTT 60

Query: 61  EFYYFPPEEKLRFDMSGGKRGGFMISTRRQGDVEDWREVVTFFTYPSRDRKYSEWPDKPE 120
           EFYY PPEEKLRFDMSGGK+GGFMISTRR+GDVEDWREVVTFFTYPSRDR+YSEWPDKPE
Sbjct: 61  EFYYLPPEEKLRFDMSGGKKGGFMISTRRKGDVEDWREVVTFFTYPSRDRRYSEWPDKPE 120

Query: 121 GWIKTTEEFSEKLMDLASKILEVLSEAMDMEKGALKKACGELNQKILTNFYPKCSNTDLE 180
           GWIKTTEEFSE+LMDLASKILEVLSEAMD+EKGALKKACGELNQKILTNFYPKCSN+DLE
Sbjct: 121 GWIKTTEEFSERLMDLASKILEVLSEAMDLEKGALKKACGELNQKILTNFYPKCSNSDLE 180

Query: 181 LGLPRHTDPGTITILLQDQVGGLQATKDGAKTWINIPPMEGAFVINIGDHGHYLSNGRFR 240
           LGLPRHTDPGTITILLQDQVGGLQATKDGA +WINIPPM+GAFVINIGDHGHYLSNGRF+
Sbjct: 181 LGLPRHTDPGTITILLQDQVGGLQATKDGANSWINIPPMKGAFVINIGDHGHYLSNGRFK 240

Query: 241 SADHRALANPKSDRLSIATFQYPTPEAIVYPLKVGEGEKAVIEDPISFAEMYKKKMSNEP 300
           SADHRALAN KSDRLSIATFQYPTPEAIVYPLKVGEGEKAVIE+PISFAEMYKKKMSNEP
Sbjct: 241 SADHRALANAKSDRLSIATFQYPTPEAIVYPLKVGEGEKAVIENPISFAEMYKKKMSNEP 300

Query: 301 VLAMFNHKGQNKLNPST 318
           VLAMFNHKG NK+NPST
Sbjct: 301 VLAMFNHKGHNKINPST 317

[Close]
BLAST of CSPI06G08660 vs. NCBI nr
Match: gi|1006592088|gb|AMQ48666.1| (flavanone 3-hydroxylase [Iochroma grandiflorum])

HSP 1 Score: 436.4 bits (1121), Expect = 4.2e-119
Identity = 200/298 (67.11%), Postives = 248/298 (83.22%), Query Frame = 1 

Query: 7   DEIPVISLDGIEDDAHMAAVKKKIVEACEVWGIFQVVDHGVDPKLIADMVRLTTEFYYFP 66
           DEIPVISL G+++      + +KIV+ACE WG+FQVVDHGVD +LI+ M +   EF+  P
Sbjct: 36  DEIPVISLKGLDESGKRGEICEKIVQACEDWGVFQVVDHGVDDELISQMTKFAKEFFALP 95

Query: 67  PEEKLRFDMSGGKRGGFMISTRRQGDV-EDWREVVTFFTYPSRDRKYSEWPDKPEGWIKT 126
           PEEKLRFDMSGGK+GGF++S+  QG+V +DWRE+VT+F+YP+R R YS WPDKP+GWI  
Sbjct: 96  PEEKLRFDMSGGKKGGFIVSSHLQGEVVQDWREIVTYFSYPTRARDYSRWPDKPQGWIAV 155

Query: 127 TEEFSEKLMDLASKILEVLSEAMDMEKGALKKACGELNQKILTNFYPKCSNTDLELGLPR 186
           TE++SEKLMDLA K+LEVLSEAM +EK AL KAC +++QK++ NFYPKC   DL LGL R
Sbjct: 156 TEKYSEKLMDLACKLLEVLSEAMGLEKEALTKACVDMDQKVVVNFYPKCPQPDLTLGLKR 215

Query: 187 HTDPGTITILLQDQVGGLQATKDGAKTWINIPPMEGAFVINIGDHGHYLSNGRFRSADHR 246
           HTDPGTIT+LLQDQVGGLQATKD  KTWI + P+EGAFV+N+GDHGHYLSNGRF++ADH+
Sbjct: 216 HTDPGTITLLLQDQVGGLQATKDNGKTWITVQPVEGAFVVNLGDHGHYLSNGRFKNADHQ 275

Query: 247 ALANPKSDRLSIATFQYPTPEAIVYPLKVGEGEKAVIEDPISFAEMYKKKMSNEPVLA 304
           A+ N  S RLSIATFQ P PEAIVYPLK+ EGEKAV+++PI+FAEMY++KMS +  LA
Sbjct: 276 AVVNSNSSRLSIATFQNPAPEAIVYPLKIREGEKAVMDEPITFAEMYRRKMSKDLELA 333

[Close]
BLAST of CSPI06G08660 vs. NCBI nr
Match: gi|1006592070|gb|AMQ48657.1| (flavanone 3-hydroxylase [Iochroma umbellatum])

HSP 1 Score: 435.3 bits (1118), Expect = 9.4e-119
Identity = 200/298 (67.11%), Postives = 247/298 (82.89%), Query Frame = 1 

Query: 7   DEIPVISLDGIEDDAHMAAVKKKIVEACEVWGIFQVVDHGVDPKLIADMVRLTTEFYYFP 66
           DEIPVISL G+++      + +KIV+ACE WG+FQVVDHGVD +LI+ M     EF+  P
Sbjct: 36  DEIPVISLKGLDESGKRGEICEKIVKACEDWGVFQVVDHGVDAELISQMTNFAKEFFALP 95

Query: 67  PEEKLRFDMSGGKRGGFMISTRRQGDV-EDWREVVTFFTYPSRDRKYSEWPDKPEGWIKT 126
           PEEKLRFDMSGGK+GGF++S+  QG+V +DWRE+VT+F+YP+R R YS WPDKP+GWI  
Sbjct: 96  PEEKLRFDMSGGKKGGFIVSSHLQGEVVQDWREIVTYFSYPTRARDYSRWPDKPQGWIAV 155

Query: 127 TEEFSEKLMDLASKILEVLSEAMDMEKGALKKACGELNQKILTNFYPKCSNTDLELGLPR 186
           TE++SEKLMDLA K+LEVLSEAM +EK AL KAC +++QK++ NFYPKC   DL LGL R
Sbjct: 156 TEKYSEKLMDLACKLLEVLSEAMGLEKEALTKACVDMDQKVVVNFYPKCPQPDLTLGLKR 215

Query: 187 HTDPGTITILLQDQVGGLQATKDGAKTWINIPPMEGAFVINIGDHGHYLSNGRFRSADHR 246
           HTDPGTIT+LLQDQVGGLQATKD  KTWI + P+EGAFV+N+GDHGHYLSNGRF++ADH+
Sbjct: 216 HTDPGTITLLLQDQVGGLQATKDNGKTWITVQPVEGAFVVNLGDHGHYLSNGRFKNADHQ 275

Query: 247 ALANPKSDRLSIATFQYPTPEAIVYPLKVGEGEKAVIEDPISFAEMYKKKMSNEPVLA 304
           A+ N  S RLSIATFQ P PEAIVYPLK+ EGEKAV+++PI+FAEMY++KMS +  LA
Sbjct: 276 AVVNSNSSRLSIATFQNPAPEAIVYPLKIREGEKAVMDEPITFAEMYRRKMSKDLELA 333

[Close]
BLAST of CSPI06G08660 vs. NCBI nr
Match: gi|325551317|gb|ADZ28514.1| (flavanone 3-hydroxylase [Camellia nitidissima])

HSP 1 Score: 433.0 bits (1112), Expect = 4.7e-118
Identity = 204/309 (66.02%), Postives = 249/309 (80.58%), Query Frame = 1 

Query: 7   DEIPVISLDGIED-DAHMAAVKKKIVEACEVWGIFQVVDHGVDPKLIADMVRLTTEFYYF 66
           +EIPVISL GI++ +   + + +KIVEACE WG+FQVVDHGVD  LIA+M RL  EF+  
Sbjct: 37  NEIPVISLAGIDEIEGRRSEICRKIVEACEDWGVFQVVDHGVDANLIAEMTRLAREFFAL 96

Query: 67  PPEEKLRFDMSGGKRGGFMISTRRQGD-VEDWREVVTFFTYPSRDRKYSEWPDKPEGWIK 126
           PPEEKLRFDMSGGK+GGF++S+  QG+ V+DWRE+VT+F+YP R R YS WPDKPEGW  
Sbjct: 97  PPEEKLRFDMSGGKKGGFIVSSHLQGEAVQDWREIVTYFSYPIRARDYSRWPDKPEGWSA 156

Query: 127 TTEEFSEKLMDLASKILEVLSEAMDMEKGALKKACGELNQKILTNFYPKCSNTDLELGLP 186
            TE +SEKLMDLA K+LEVLSEAM +EK AL KAC +++QK++ NFYPKC   DL LGL 
Sbjct: 157 VTETYSEKLMDLACKLLEVLSEAMGLEKEALTKACVDMDQKVVINFYPKCPQPDLTLGLK 216

Query: 187 RHTDPGTITILLQDQVGGLQATKDGAKTWINIPPMEGAFVINIGDHGHYLSNGRFRSADH 246
           RHTDPGTIT+LLQDQVGGLQAT+DG KTWI + P+EGAFV+N+GDHGHYLSNGRF++ADH
Sbjct: 217 RHTDPGTITLLLQDQVGGLQATRDGGKTWITVQPVEGAFVVNLGDHGHYLSNGRFKNADH 276

Query: 247 RALANPKSDRLSIATFQYPTPEAIVYPLKVGEGEKAVIEDPISFAEMYKKKMSNEPVLAM 306
           +A+ N    RLSIATFQ P PEA VYPLK+ EGEK V+E+PI+FA+MYK+KMS +  LA 
Sbjct: 277 QAVVNSNCSRLSIATFQNPAPEATVYPLKIREGEKPVLEEPITFADMYKRKMSKDIELAK 336

Query: 307 FNHKGQNKL 314
                + KL
Sbjct: 337 LKKLAKEKL 345

The following BLAST results are available for this feature:
BLAST of CSPI06G08660 vs. Swiss-Prot
Match NameE-valueIdentityDescription
FL3H_ARATH5.0e-12066.89Naringenin,2-oxoglutarate 3-dioxygenase OS=Arabidopsis thaliana GN=F3H PE=1 SV=1[more]
FL3H_MATIN2.5e-11965.16Naringenin,2-oxoglutarate 3-dioxygenase (Fragment) OS=Matthiola incana GN=FHT PE... [more]
FL3H_PETHY3.6e-11863.31Naringenin,2-oxoglutarate 3-dioxygenase (Fragment) OS=Petunia hybrida GN=AN3 PE=... [more]
FL3H_PETCR9.7e-11662.90Flavanone 3-dioxygenase OS=Petroselinum crispum GN=FHT PE=1 SV=1[more]
FL3H_VITVI1.7e-11565.56Naringenin,2-oxoglutarate 3-dioxygenase OS=Vitis vinifera GN=F3H PE=2 SV=1[more]
BLAST of CSPI06G08660 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0KAQ6_CUCSA3.4e-18499.68Flavanone-3-hydroxylase OS=Cucumis sativus GN=Csa_6G108510 PE=3 SV=1[more]
A0A142EGS0_9SOLA2.9e-11967.11Flavanone 3-hydroxylase OS=Iochroma grandiflorum PE=2 SV=1[more]
A0A142EGR1_9SOLA6.6e-11967.11Flavanone 3-hydroxylase OS=Iochroma umbellatum PE=2 SV=1[more]
F1CJI9_9ERIC3.3e-11866.02Flavanone 3-hydroxylase OS=Camellia nitidissima GN=F3H PE=2 SV=1[more]
A0A142EGQ7_9SOLA3.3e-11867.11Flavanone 3-hydroxylase OS=Iochroma tupayachianum PE=2 SV=1[more]
BLAST of CSPI06G08660 vs. TAIR10
Match NameE-valueIdentityDescription
AT3G51240.12.8e-12166.89 flavanone 3-hydroxylase[more]
AT5G24530.11.5e-4534.80 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot... [more]
AT1G55290.11.1e-4334.15 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot... [more]
AT4G10500.11.4e-4331.51 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot... [more]
AT3G21420.14.0e-4333.33 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily prot... [more]
BLAST of CSPI06G08660 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|449445471|ref|XP_004140496.1|4.9e-18499.68PREDICTED: naringenin,2-oxoglutarate 3-dioxygenase-like [Cucumis sativus][more]
gi|659119588|ref|XP_008459735.1|5.4e-17593.69PREDICTED: naringenin,2-oxoglutarate 3-dioxygenase-like [Cucumis melo][more]
gi|1006592088|gb|AMQ48666.1|4.2e-11967.11flavanone 3-hydroxylase [Iochroma grandiflorum][more]
gi|1006592070|gb|AMQ48657.1|9.4e-11967.11flavanone 3-hydroxylase [Iochroma umbellatum][more]
gi|325551317|gb|ADZ28514.1|4.7e-11866.02flavanone 3-hydroxylase [Camellia nitidissima][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR005123Oxoglu/Fe-dep_dioxygenase
IPR026992DIOX_N
IPR027443IPNS-like
Vocabulary: Molecular Function
TermDefinition
GO:0016491oxidoreductase activity
Vocabulary: Biological Process
TermDefinition
GO:0055114oxidation-reduction process
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009813 flavonoid biosynthetic process
biological_process GO:0055114 oxidation-reduction process
biological_process GO:0010224 response to UV-B
cellular_component GO:0005575 cellular_component
molecular_function GO:0031418 L-ascorbic acid binding
molecular_function GO:0046872 metal ion binding
molecular_function GO:0045486 naringenin 3-dioxygenase activity
molecular_function GO:0016491 oxidoreductase activity
molecular_function GO:0005524 ATP binding
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI06G08660.1CSPI06G08660.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005123Oxoglutarate/iron-dependent dioxygenasePFAMPF031712OG-FeII_Oxycoord: 166..260
score: 1.1
IPR005123Oxoglutarate/iron-dependent dioxygenasePROFILEPS51471FE2OG_OXYcoord: 162..263
score: 13
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 9..117
score: 8.9
IPR027443Isopenicillin N synthase-likeGENE3DG3DSA:2.60.120.330coord: 5..298
score: 2.4E
NoneNo IPR availablePANTHERPTHR10209OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEINcoord: 7..305
score: 2.7E
NoneNo IPR availablePANTHERPTHR10209:SF137NARINGENIN,2-OXOGLUTARATE 3-DIOXYGENASEcoord: 7..305
score: 2.7E
NoneNo IPR availableunknownSSF51197Clavaminate synthase-likecoord: 5..297
score: 4.81