CSPI06G06320 (gene) Wild cucumber (PI 183967) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CSPI06G06320
Type	gene
Organism	Cucumis sativus (Wild cucumber (PI 183967))
Description	Plant invertase/pectin methylesterase inhibitor
Location	Chr6 : 5535447 .. 5536013 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCATACCTCATTATTTATCTGTTTTTATTCTCTCAGTTTTCTTCTTCTCCAACTTCCTCATTATACAATCCTCCAAAACCATCAAAACAGCTGATCTAATCTACAAAACTTGCAAGAAAATCTCAAGAGAAGACCCTAACGTTTCCTTCAACTTTTGCTTAGCATCTCTAAAACTTGCCACCAACCATAGCCGATGCACCGACGTTCGCCACCTCGGCCTATTCTCGATTGGGTTCTTATGTAGGAATGTGACCAGCACGTACCACCATATCACAAAGTTGGTGAGGAACAAGAAGCTCGACCCTTTTGTTAAGTTATGTTTGGATGATTGTTTGGAGCTTTATACCGATGCGATACCAACGGTGAAGCAAGCGATGAAGGATTATAAGTCGAAGAGATATGACGATGCGAATGTAGCTATTAGTTCGGTGATGGATGCAGCAACGACTTGTGAGGATGGGTTTAAGGAGAGGAAAGGGGTAGCTTCACCATTGAAGAAGAGGGATGGAGATGCTTTTGAATTGGGAGCCATTGCATTGTCAATTATGAGTTTGCTTGGTTGA

mRNA sequence

ATGGCCATACCTCATTATTTATCTGTTTTTATTCTCTCAGTTTTCTTCTTCTCCAACTTCCTCATTATACAATCCTCCAAAACCATCAAAACAGCTGATCTAATCTACAAAACTTGCAAGAAAATCTCAAGAGAAGACCCTAACGTTTCCTTCAACTTTTGCTTAGCATCTCTAAAACTTGCCACCAACCATAGCCGATGCACCGACGTTCGCCACCTCGGCCTATTCTCGATTGGGTTCTTATGTAGGAATGTGACCAGCACGTACCACCATATCACAAAGTTGGTGAGGAACAAGAAGCTCGACCCTTTTGTTAAGTTATGTTTGGATGATTGTTTGGAGCTTTATACCGATGCGATACCAACGGTGAAGCAAGCGATGAAGGATTATAAGTCGAAGAGATATGACGATGCGAATGTAGCTATTAGTTCGGTGATGGATGCAGCAACGACTTGTGAGGATGGGTTTAAGGAGAGGAAAGGGGTAGCTTCACCATTGAAGAAGAGGGATGGAGATGCTTTTGAATTGGGAGCCATTGCATTGTCAATTATGAGTTTGCTTGGTTGA

Coding sequence (CDS)

ATGGCCATACCTCATTATTTATCTGTTTTTATTCTCTCAGTTTTCTTCTTCTCCAACTTCCTCATTATACAATCCTCCAAAACCATCAAAACAGCTGATCTAATCTACAAAACTTGCAAGAAAATCTCAAGAGAAGACCCTAACGTTTCCTTCAACTTTTGCTTAGCATCTCTAAAACTTGCCACCAACCATAGCCGATGCACCGACGTTCGCCACCTCGGCCTATTCTCGATTGGGTTCTTATGTAGGAATGTGACCAGCACGTACCACCATATCACAAAGTTGGTGAGGAACAAGAAGCTCGACCCTTTTGTTAAGTTATGTTTGGATGATTGTTTGGAGCTTTATACCGATGCGATACCAACGGTGAAGCAAGCGATGAAGGATTATAAGTCGAAGAGATATGACGATGCGAATGTAGCTATTAGTTCGGTGATGGATGCAGCAACGACTTGTGAGGATGGGTTTAAGGAGAGGAAAGGGGTAGCTTCACCATTGAAGAAGAGGGATGGAGATGCTTTTGAATTGGGAGCCATTGCATTGTCAATTATGAGTTTGCTTGGTTGA

Homology

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BLAST of CSPI06G06320 vs. Swiss-Prot
Match: PLA1_PLAAC (Putative invertase inhibitor OS=Platanus acerifolia PE=1 SV=1)

HSP 1 Score: 135.6 bits (340), Expect = 5.7e-31
Identity = 72/179 (40.22%), Postives = 119/179 (66.48%), Query Frame = 1


Query: 10  FILSVFFFSNFLIIQSSKTIKTADLIYKTCKKISREDPNVSFNFCLASLKLATNHSRCTD 69
           F L +FFF+  L++Q+   + +AD++  TCKK+++  PNV+++FC+ SL  A   S   D
Sbjct: 5   FSLCIFFFNLLLLLQA---VISADIVQGTCKKVAQRSPNVNYDFCVKSLG-ADPKSHTAD 64

Query: 70  VRHLGLFSIGFLCRNVTSTYHHITKLVRNKKLDPFVKLCLDDCLELYTDAIPTVKQAMKD 129
           ++ LG+ S     ++ +     I +++++K +DP +K  L+DC+ LY DA  +V++A+ D
Sbjct: 65  LQGLGVISANLAIQHGSKIQTFIGRILKSK-VDPALKKYLNDCVGLYADAKSSVQEAIAD 124

Query: 130 YKSKRYDDANVAISSVMDAATTCEDGFKERKGVASPLKKRDGDAFELGAIALSIMSLLG 189
           +KSK Y  ANV +S+ +D + TCEDGFKE+KG+ SP+ K + D  +L AI+L+I  LLG
Sbjct: 125 FKSKDYASANVKMSAALDDSVTCEDGFKEKKGIVSPVTKENKDYVQLTAISLAITKLLG 178

[Close]

BLAST of CSPI06G06320 vs. Swiss-Prot
Match: CVIF2_ARATH (Cell wall / vacuolar inhibitor of fructosidase 2 OS=Arabidopsis thaliana GN=C/VIF2 PE=1 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 1.5e-07
Identity = 44/165 (26.67%), Postives = 79/165 (47.88%), Query Frame = 1


Query: 9   VFILSVFFFSNFLIIQSSKTIKTADLIYKTCKKISREDPNVSFNFCLASLKLATNHSRCT 68
           +F+L V    +   + S+K+  T  +I  TCK  +       + FC+++LK +   S   
Sbjct: 6   IFLLLVTLTFSASTLISAKS-NTTTIIESTCKTTNY------YKFCVSALK-SDPRSPTA 65

Query: 69  DVRHLGLFSIGFLCRNVTSTYHHITKLVRNKKLDPFVKLCLDDCLELYTDAIPTVKQAMK 128
           D + L    +G    N TST ++I   +     D  +K  L DC E Y  A  +++  ++
Sbjct: 66  DTKGLASIMVGVGMTNATSTANYIAGNLSATVKDTVLKKVLQDCSEKYALAADSLRLTIQ 125

Query: 129 DYKSKRYDDANVAISSVMDAATTCEDGFKERKGVASPLKKRDGDA 174
           D   + YD A++ + +  D    C + F+  KG+A P++ R  +A
Sbjct: 126 DLDDEAYDYASMHVLAAQDYPNVCRNIFRRVKGLAYPVEIRRREA 162

[Close]

BLAST of CSPI06G06320 vs. Swiss-Prot
Match: PMEI_ACTDE (Pectinesterase inhibitor OS=Actinidia deliciosa GN=PMEI PE=1 SV=2)

HSP 1 Score: 56.6 bits (135), Expect = 3.4e-07
Identity = 55/189 (29.10%), Postives = 84/189 (44.44%), Query Frame = 1


Query: 1   MAIPHYLSVFILSVFFFSNFLIIQSSKTIKTAD--LIYKTCKKISREDPNVSFNFCLASL 60
           MA  +  S   +S+     F+   S +    A+  LI + C K          + CL +L
Sbjct: 1   MAFSYCSSSLFVSLLLVILFISPLSQRPSVKAENHLISEICPKTRNP------SLCLQAL 60

Query: 61  KLATNHSRCTDVRHLGLFSIGFLCRNVTSTYHHITKLVRNKKLDPFVKLCLDDCLELYTD 120
           + +   S   D++ LG FSI     +   T   I  L  N+  DP +K   + C E Y D
Sbjct: 61  E-SDPRSASKDLKGLGQFSIDIAQASAKQTSKIIASLT-NQATDPKLKGRYETCSENYAD 120

Query: 121 AIPTVKQAMKDYKSKRYDDANVAISSVMDAATTCEDGFKERKGVASPLKKRDGDAFELGA 180
           AI ++ QA +   S  Y+  N+  S+  D A TCED F+    + + L + D    +L  
Sbjct: 121 AIDSLGQAKQFLTSGDYNSLNIYASAAFDGAGTCEDSFEGPPNIPTQLHQADLKLEDLCD 180

Query: 181 IALSIMSLL 188
           I L I +LL
Sbjct: 181 IVLVISNLL 181

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BLAST of CSPI06G06320 vs. TrEMBL
Match: A0A0A0K951_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G080380 PE=4 SV=1)

HSP 1 Score: 369.8 bits (948), Expect = 2.0e-99
Identity = 186/188 (98.94%), Postives = 186/188 (98.94%), Query Frame = 1


Query: 1   MAIPHYLSVFILSVFFFSNFLIIQSSKTIKTADLIYKTCKKISREDPNVSFNFCLASLKL 60
           MAIPHY SVFILSVFFFSNFLIIQSSKTIKTADLIYKTCKKISREDPNVSFNFCLASLKL
Sbjct: 1   MAIPHYSSVFILSVFFFSNFLIIQSSKTIKTADLIYKTCKKISREDPNVSFNFCLASLKL 60

Query: 61  ATNHSRCTDVRHLGLFSIGFLCRNVTSTYHHITKLVRNKKLDPFVKLCLDDCLELYTDAI 120
           A NHSRCTDVRHLGLFSIGFLCRNVTSTYHHITKLVRNKKLDPFVKLCLDDCLELYTDAI
Sbjct: 61  AANHSRCTDVRHLGLFSIGFLCRNVTSTYHHITKLVRNKKLDPFVKLCLDDCLELYTDAI 120

Query: 121 PTVKQAMKDYKSKRYDDANVAISSVMDAATTCEDGFKERKGVASPLKKRDGDAFELGAIA 180
           PTVKQAMKDYKSKRYDDANVAISSVMDAATTCEDGFKERKGVASPLKKRDGDAFELGAIA
Sbjct: 121 PTVKQAMKDYKSKRYDDANVAISSVMDAATTCEDGFKERKGVASPLKKRDGDAFELGAIA 180

Query: 181 LSIMSLLG 189
           LSIMSLLG
Sbjct: 181 LSIMSLLG 188

[Close]

BLAST of CSPI06G06320 vs. TrEMBL
Match: A0A0A0KBR8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G080370 PE=4 SV=1)

HSP 1 Score: 199.9 bits (507), Expect = 2.8e-48
Identity = 107/190 (56.32%), Postives = 133/190 (70.00%), Query Frame = 1


Query: 2   AIPHYLSVFILSVFFFSNFLIIQSSKTIKTADLIYKTCKKISREDPNVSFNFCLASLKLA 61
           ++ H +   +    FFSNF I QSS    T+ LIYKTCK  S +DPN+SFNFC+ SLK A
Sbjct: 3   SLSHSILPCLFLFMFFSNFPITQSSNNNNTSSLIYKTCKASSEQDPNISFNFCVTSLKPA 62

Query: 62  TNHSRC--TDVRHLGLFSIGFLCRNVTSTYHHITKLVRNKK--LDPFVKLCLDDCLELYT 121
               R   T +R LGL +I  +  N+++T HHI K +   K   DPFVKLCL DCLELY+
Sbjct: 63  ATKHRHGDTSLRRLGLITIYLIRHNMSNTRHHIKKYLHKNKGPRDPFVKLCLTDCLELYS 122

Query: 122 DAIPTVKQAMKDYKSKRYDDANVAISSVMDAATTCEDGFKERKGVASPLKKRDGDAFELG 181
           DAIPTVKQA KDYK+ RY DAN+ ISSVMD  +TCE+GFKE+ GV SPL  R+ +AFEL 
Sbjct: 123 DAIPTVKQARKDYKAGRYADANLKISSVMDDCSTCEEGFKEKDGVISPLTNRNHNAFELS 182

Query: 182 AIALSIMSLL 188
           AIALSI+++L
Sbjct: 183 AIALSIINML 192

[Close]

BLAST of CSPI06G06320 vs. TrEMBL
Match: A0A0D2PD74_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_004G168200 PE=4 SV=1)

HSP 1 Score: 197.6 bits (501), Expect = 1.4e-47
Identity = 100/178 (56.18%), Postives = 131/178 (73.60%), Query Frame = 1


Query: 12  LSVFFFSNFLIIQSSKTIKTADLIYKTCKKISREDPNVSFNFCLASLKLATNHSRC-TDV 71
           L +FFF   +      T     LI KTCKK ++ DPN+S+NFC+ SL+ A  HS C  D+
Sbjct: 15  LCIFFFFVSISTSHGLTATNDSLIRKTCKKCAQSDPNLSYNFCITSLQ-AAPHSHCANDL 74

Query: 72  RHLGLFSIGFLCRNVTSTYHHITKLVRN-KKLDPFVKLCLDDCLELYTDAIPTVKQAMKD 131
           R LG  SI  L RNVT+T  HI +L++N KK+DPFV+ CL DC +LY+DAIPT KQA++D
Sbjct: 75  RQLGKISITLLGRNVTNTRSHIKELLKNQKKMDPFVRSCLHDCFDLYSDAIPTTKQALQD 134

Query: 132 YKSKRYDDANVAISSVMDAATTCEDGFKERKGVASPLKKRDGDAFELGAIALSIMSLL 188
           YK+K YDDAN+ +SSVMDA TTCEDGFKE++G+ SPL KR+ DAF L AI+LSI++++
Sbjct: 135 YKAKHYDDANIDVSSVMDATTTCEDGFKEKEGLVSPLTKRNNDAFMLSAISLSIINMI 191

[Close]

BLAST of CSPI06G06320 vs. TrEMBL
Match: B9MVC0_POPTR (Invertase/pectin methylesterase inhibitor family protein OS=Populus trichocarpa GN=POPTR_0014s04360g PE=4 SV=1)

HSP 1 Score: 194.9 bits (494), Expect = 8.9e-47
Identity = 88/155 (56.77%), Postives = 127/155 (81.94%), Query Frame = 1


Query: 33  DLIYKTCKKISREDPNVSFNFCLASLKLATNHSRCTDVRHLGLFSIGFLCRNVTSTYHHI 92
           +LI +TCKK +  DPN+S+NFC+ SL+ A+N S+C ++R LG+ SI  +  NVT+T H++
Sbjct: 27  NLIPETCKKCAANDPNLSYNFCVTSLQ-ASNRSQCDNLRGLGMMSIKLIKYNVTNTRHYV 86

Query: 93  TKLVRNKKLDPFVKLCLDDCLELYTDAIPTVKQAMKDYKSKRYDDANVAISSVMDAATTC 152
             L++NKK+DPF++ CL+DCL+LY+DAIPT+KQAM DYKSK Y DAN+ +SSV+DAATTC
Sbjct: 87  KNLLKNKKMDPFIRACLNDCLDLYSDAIPTLKQAMIDYKSKHYKDANIEVSSVIDAATTC 146

Query: 153 EDGFKERKGVASPLKKRDGDAFELGAIALSIMSLL 188
           EDGF++++G  SPL KR+ D F+L AIAL+++++L
Sbjct: 147 EDGFEDKEGAVSPLTKRNNDTFQLSAIALALINML 180

[Close]

BLAST of CSPI06G06320 vs. TrEMBL
Match: E5GC99_CUCME (Invertase inhibitor OS=Cucumis melo subsp. melo PE=4 SV=1)

HSP 1 Score: 191.4 bits (485), Expect = 9.8e-46
Identity = 105/178 (58.99%), Postives = 128/178 (71.91%), Query Frame = 1


Query: 14  VFFFSNFLIIQSSKTIK-TADLIYKTCKKISREDPNVSFNFCLASLKLATNHSRC--TDV 73
           +  FSNF I QSS     T+ LIYKTCK  S +DPN+SF+FC+ SLK A    R   T +
Sbjct: 1   MLIFSNFPITQSSNNNNNTSSLIYKTCKASSEQDPNISFDFCITSLKPAATKHRHGDTSL 60

Query: 74  RHLGLFSIGFLCRNVTSTYHHITKLVRNKKL-DPFVKLCLDDCLELYTDAIPTVKQAMKD 133
           R LGL +I  +  N++ST HHI KL R K   DPFVKLCL DCLELY+DAIP VKQA KD
Sbjct: 61  RRLGLMTIYLIRHNMSSTRHHIKKLQRKKGPGDPFVKLCLTDCLELYSDAIPMVKQARKD 120

Query: 134 YKSKRYDDANVAISSVMDAATTCEDGFKERKGVASPLKKRDGDAFELGAIALSIMSLL 188
           YK+ RY DAN  ISSVMD  +TCE+GF+E++GV SPL  R+ +AFEL AIALSI+++L
Sbjct: 121 YKAGRYADANSKISSVMDDCSTCEEGFEEKEGVVSPLTNRNHNAFELSAIALSIINML 178

[Close]

BLAST of CSPI06G06320 vs. TAIR10
Match: AT5G46940.1 (AT5G46940.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 94.4 bits (233), Expect = 8.3e-20
Identity = 49/154 (31.82%), Postives = 82/154 (53.25%), Query Frame = 1


Query: 34  LIYKTCKKISREDPNVSFNFCLASLKLATNHSRCTDVRHLGLFSIGFLCRNVTSTYHHIT 93
           LI  +CKK +   P   +N C+ SL+         D+  L + S        T+    + 
Sbjct: 23  LIRNSCKKATATSPKFKYNLCVTSLETNPQAKTAKDLAGLVMASTKNAVTKATTLKGTVD 82

Query: 94  KLVRNKKLDPFVKLCLDDCLELYTDAIPTVKQAMKDYKSKRYDDANVAISSVMDAATTCE 153
           K+++ KK++    + L DCL+LYTDAI ++ +A+   KS+ Y      +S+ MD  +TCE
Sbjct: 83  KIIKGKKVNKMTAMPLRDCLQLYTDAIGSLNEALAGVKSRNYPTVKTVLSAAMDTPSTCE 142

Query: 154 DGFKERKGVASPLKKRDGDAFELGAIALSIMSLL 188
            GFKERK   SP+ K + + +++  I L+  ++L
Sbjct: 143 TGFKERK-APSPVTKENDNLYQMILIPLAFTNML 175

[Close]

BLAST of CSPI06G06320 vs. TAIR10
Match: AT5G46970.1 (AT5G46970.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 88.2 bits (217), Expect = 5.9e-18
Identity = 52/156 (33.33%), Postives = 87/156 (55.77%), Query Frame = 1


Query: 14  VFFFSNFLIIQSSKTIKTADLIYKTCKKISREDPNVSFNFCLASLKLATNHSRCTDVRHL 73
           +F+F+ F ++ ++ TI  + LI   CKK + ++P + +NFC+ SL+          +  L
Sbjct: 4   LFYFAMFFLLLNAFTIAQS-LIQDFCKKAADKNPKIHYNFCVKSLEENPQSKTARSLDRL 63

Query: 74  GLFSIGFLCRNVTSTYHHITKLVRNKKLDPFVKLCLDDCLELYTDAIPTVKQAMKDYKSK 133
            + S        TS    + K+++  + + + +  L DCLELY+DA  ++K+A+   KS+
Sbjct: 64  VMLSTKNAVSKTTSMKGIVDKILKENRFEMYSEKPLRDCLELYSDATNSLKEALTIIKSR 123

Query: 134 RYDDANVAISSVMDAATTCEDGFKE-RKGVASPLKK 169
            Y  ANV IS+ M A  +CE GFKE +K + SP  K
Sbjct: 124 DYKTANVVISAAMGAPPSCEIGFKEGKKPLKSPFTK 158

[Close]

BLAST of CSPI06G06320 vs. TAIR10
Match: AT5G38610.1 (AT5G38610.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 86.3 bits (212), Expect = 2.2e-17
Identity = 64/192 (33.33%), Postives = 98/192 (51.04%), Query Frame = 1


Query: 12  LSVFFFSNFLIIQSSKTIKTADLIYKTCKKISREDPNVSFNFCLASLKLATNHSRC---- 71
           LS  F+  FL    S+   T+D+I +TCK  + +     +NFC++SL    N+S      
Sbjct: 3   LSQVFYIIFLFFLVSQVKSTSDMIDQTCKSCAAKSTIFDYNFCVSSL----NNSPIALPS 62

Query: 72  -TDVRHLGLFSIGFLCRNVTSTYHHITKLVRNKKLDPFVKLCLDDCLELYTDAIPTVKQA 131
            T++  L L  +     N T+T   I +L+ +     F   CL DCLELY DA   +++A
Sbjct: 63  PTNLSSLALVPMLQALDNATATASTIQQLLISDDDGSFRSACLRDCLELYEDATDRLEEA 122

Query: 132 MKDY-KSKRYDDANVAISSVMDAATTCEDGFKERK-------GV---ASPLKKRDGDAFE 188
           ++ +   K     NV +S+ M++A TCE+GF+ER        GV    SP+   +   FE
Sbjct: 123 VRVFITRKELGTVNVMVSAAMESAVTCENGFRERDDGDGGGGGVTTWTSPIGDENHKLFE 182

[Close]

BLAST of CSPI06G06320 vs. TAIR10
Match: AT5G46930.1 (AT5G46930.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 81.3 bits (199), Expect = 7.2e-16
Identity = 54/176 (30.68%), Postives = 91/176 (51.70%), Query Frame = 1


Query: 13  SVFFFSNFLIIQSSKTIKTADLIYKTCKKISREDPNVSFNFCLASLKLATNHSRCTDVRH 72
           S++    FL++    T ++  LI  +CKK + +DP + ++FC+ SL+          +  
Sbjct: 4   SLYLVMFFLLLNCFATAQS--LIRDSCKKATTKDPKLKYDFCVKSLEENPQSKTAKSLEG 63

Query: 73  LGLFSIGFLCRNVTSTYHHITKLVRNKKLDPFVKLCLDDCLELYTDAIPTVKQAMKDYKS 132
           L   S        TS    + K+++  K +  V+  L DCLELYT+AI ++ Q++   KS
Sbjct: 64  LVFVSTKNAVSKTTSLKGMVDKILKEDKYE--VERPLLDCLELYTEAIDSLNQSLDTVKS 123

Query: 133 KRYDDANVAISSVMDAATTCEDGF-KERKGVASPLKKRDGDAFELGAIALSIMSLL 188
           + Y  A + +S+ MDA  +CE  F K +K V SP  K +   F +  I ++  S+L
Sbjct: 124 RDYKTATMLMSAAMDAPGSCETKFTKRKKAVKSPFTKENDVLFYMVLIPIAFTSML 175

[Close]

BLAST of CSPI06G06320 vs. TAIR10
Match: AT5G46950.1 (AT5G46950.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 73.2 bits (178), Expect = 2.0e-13
Identity = 53/178 (29.78%), Postives = 91/178 (51.12%), Query Frame = 1


Query: 10  FILSVFFFSNFLIIQSSKTIKTADLIYKTCKKISREDPNVSFNFCLASLKLATNHSRCTD 69
           F++S+  FS FL      T +T  LI  +CKK   +DP  S++FC+ SL         T 
Sbjct: 3   FLVSLVIFSLFL--NGFATAQT--LIQDSCKKAFAKDPQSSYDFCVQSLTQDPQSKAATT 62

Query: 70  VRHLGLFSIGFLCRNVTSTYHHITKLVRNKKLDPFVKLCLDDCLELYTDAIPTVKQAMKD 129
           +  L L S   +   +T+    + + +++++    V+  L  CL  Y DA  ++K A+ +
Sbjct: 63  LEDLALASTKNVAAKITNLKGIVAQDLKDQRYQDIVE-DLKLCLGFYKDANDSLKTALAN 122

Query: 130 YKSKRYDDANVAISSVMDAATTCEDGFKERKGVASPLKKRDGDAFELGAIALSIMSLL 188
            KS+ YD AN  +S+ ++A   CED FKE +   SP+   +   ++   I L+  ++L
Sbjct: 123 IKSRDYDGANSNLSAALNAPGDCEDDFKEAE-KKSPITNENNILYKTIVIPLAFTTML 174

[Close]

BLAST of CSPI06G06320 vs. NCBI nr
Match: gi|449454568|ref|XP_004145026.1| (PREDICTED: putative invertase inhibitor [Cucumis sativus])

HSP 1 Score: 369.8 bits (948), Expect = 2.9e-99
Identity = 186/188 (98.94%), Postives = 186/188 (98.94%), Query Frame = 1


Query: 1   MAIPHYLSVFILSVFFFSNFLIIQSSKTIKTADLIYKTCKKISREDPNVSFNFCLASLKL 60
           MAIPHY SVFILSVFFFSNFLIIQSSKTIKTADLIYKTCKKISREDPNVSFNFCLASLKL
Sbjct: 1   MAIPHYSSVFILSVFFFSNFLIIQSSKTIKTADLIYKTCKKISREDPNVSFNFCLASLKL 60

Query: 61  ATNHSRCTDVRHLGLFSIGFLCRNVTSTYHHITKLVRNKKLDPFVKLCLDDCLELYTDAI 120
           A NHSRCTDVRHLGLFSIGFLCRNVTSTYHHITKLVRNKKLDPFVKLCLDDCLELYTDAI
Sbjct: 61  AANHSRCTDVRHLGLFSIGFLCRNVTSTYHHITKLVRNKKLDPFVKLCLDDCLELYTDAI 120

Query: 121 PTVKQAMKDYKSKRYDDANVAISSVMDAATTCEDGFKERKGVASPLKKRDGDAFELGAIA 180
           PTVKQAMKDYKSKRYDDANVAISSVMDAATTCEDGFKERKGVASPLKKRDGDAFELGAIA
Sbjct: 121 PTVKQAMKDYKSKRYDDANVAISSVMDAATTCEDGFKERKGVASPLKKRDGDAFELGAIA 180

Query: 181 LSIMSLLG 189
           LSIMSLLG
Sbjct: 181 LSIMSLLG 188

[Close]

BLAST of CSPI06G06320 vs. NCBI nr
Match: gi|659120165|ref|XP_008460048.1| (PREDICTED: putative invertase inhibitor [Cucumis melo])

HSP 1 Score: 339.7 bits (870), Expect = 3.2e-90
Identity = 170/187 (90.91%), Postives = 176/187 (94.12%), Query Frame = 1


Query: 1   MAIPHYLSVFILSVFFFSNFLIIQSSKTIKTADLIYKTCKKISREDPNVSFNFCLASLKL 60
           MAIPHY SVFILS+FFFSNFLIIQSSKTIKTADLIYKTCKKISREDPN+SFNFCL SLKL
Sbjct: 1   MAIPHYFSVFILSIFFFSNFLIIQSSKTIKTADLIYKTCKKISREDPNISFNFCLTSLKL 60

Query: 61  ATNHSRCTDVRHLGLFSIGFLCRNVTSTYHHITKLVRNKKLDPFVKLCLDDCLELYTDAI 120
           ATNHSRCTDVRHLGL SIG L RNVTST HHITKLV+NKKLDPFVK CLDDCLELY+DAI
Sbjct: 61  ATNHSRCTDVRHLGLLSIGLLYRNVTSTCHHITKLVKNKKLDPFVKSCLDDCLELYSDAI 120

Query: 121 PTVKQAMKDYKSKRYDDANVAISSVMDAATTCEDGFKERKGVASPLKKRDGDAFELGAIA 180
           PTVKQAMKDYKSKRYDD NVAI SVMDAATTCEDGFKERKGVASPLKKRD +AFELGAI 
Sbjct: 121 PTVKQAMKDYKSKRYDDVNVAIGSVMDAATTCEDGFKERKGVASPLKKRDENAFELGAIV 180

Query: 181 LSIMSLL 188
           LSIMSL+
Sbjct: 181 LSIMSLV 187

[Close]

BLAST of CSPI06G06320 vs. NCBI nr
Match: gi|449454566|ref|XP_004145025.1| (PREDICTED: putative invertase inhibitor [Cucumis sativus])

HSP 1 Score: 199.9 bits (507), Expect = 4.0e-48
Identity = 107/190 (56.32%), Postives = 133/190 (70.00%), Query Frame = 1


Query: 2   AIPHYLSVFILSVFFFSNFLIIQSSKTIKTADLIYKTCKKISREDPNVSFNFCLASLKLA 61
           ++ H +   +    FFSNF I QSS    T+ LIYKTCK  S +DPN+SFNFC+ SLK A
Sbjct: 3   SLSHSILPCLFLFMFFSNFPITQSSNNNNTSSLIYKTCKASSEQDPNISFNFCVTSLKPA 62

Query: 62  TNHSRC--TDVRHLGLFSIGFLCRNVTSTYHHITKLVRNKK--LDPFVKLCLDDCLELYT 121
               R   T +R LGL +I  +  N+++T HHI K +   K   DPFVKLCL DCLELY+
Sbjct: 63  ATKHRHGDTSLRRLGLITIYLIRHNMSNTRHHIKKYLHKNKGPRDPFVKLCLTDCLELYS 122

Query: 122 DAIPTVKQAMKDYKSKRYDDANVAISSVMDAATTCEDGFKERKGVASPLKKRDGDAFELG 181
           DAIPTVKQA KDYK+ RY DAN+ ISSVMD  +TCE+GFKE+ GV SPL  R+ +AFEL 
Sbjct: 123 DAIPTVKQARKDYKAGRYADANLKISSVMDDCSTCEEGFKEKDGVISPLTNRNHNAFELS 182

Query: 182 AIALSIMSLL 188
           AIALSI+++L
Sbjct: 183 AIALSIINML 192

[Close]

BLAST of CSPI06G06320 vs. NCBI nr
Match: gi|823151115|ref|XP_012475386.1| (PREDICTED: putative invertase inhibitor [Gossypium raimondii])

HSP 1 Score: 197.6 bits (501), Expect = 2.0e-47
Identity = 100/178 (56.18%), Postives = 131/178 (73.60%), Query Frame = 1


Query: 12  LSVFFFSNFLIIQSSKTIKTADLIYKTCKKISREDPNVSFNFCLASLKLATNHSRC-TDV 71
           L +FFF   +      T     LI KTCKK ++ DPN+S+NFC+ SL+ A  HS C  D+
Sbjct: 15  LCIFFFFVSISTSHGLTATNDSLIRKTCKKCAQSDPNLSYNFCITSLQ-AAPHSHCANDL 74

Query: 72  RHLGLFSIGFLCRNVTSTYHHITKLVRN-KKLDPFVKLCLDDCLELYTDAIPTVKQAMKD 131
           R LG  SI  L RNVT+T  HI +L++N KK+DPFV+ CL DC +LY+DAIPT KQA++D
Sbjct: 75  RQLGKISITLLGRNVTNTRSHIKELLKNQKKMDPFVRSCLHDCFDLYSDAIPTTKQALQD 134

Query: 132 YKSKRYDDANVAISSVMDAATTCEDGFKERKGVASPLKKRDGDAFELGAIALSIMSLL 188
           YK+K YDDAN+ +SSVMDA TTCEDGFKE++G+ SPL KR+ DAF L AI+LSI++++
Sbjct: 135 YKAKHYDDANIDVSSVMDATTTCEDGFKEKEGLVSPLTKRNNDAFMLSAISLSIINMI 191

[Close]

BLAST of CSPI06G06320 vs. NCBI nr
Match: gi|566202453|ref|XP_006375099.1| (invertase/pectin methylesterase inhibitor family protein [Populus trichocarpa])

HSP 1 Score: 194.9 bits (494), Expect = 1.3e-46
Identity = 88/155 (56.77%), Postives = 127/155 (81.94%), Query Frame = 1


Query: 33  DLIYKTCKKISREDPNVSFNFCLASLKLATNHSRCTDVRHLGLFSIGFLCRNVTSTYHHI 92
           +LI +TCKK +  DPN+S+NFC+ SL+ A+N S+C ++R LG+ SI  +  NVT+T H++
Sbjct: 27  NLIPETCKKCAANDPNLSYNFCVTSLQ-ASNRSQCDNLRGLGMMSIKLIKYNVTNTRHYV 86

Query: 93  TKLVRNKKLDPFVKLCLDDCLELYTDAIPTVKQAMKDYKSKRYDDANVAISSVMDAATTC 152
             L++NKK+DPF++ CL+DCL+LY+DAIPT+KQAM DYKSK Y DAN+ +SSV+DAATTC
Sbjct: 87  KNLLKNKKMDPFIRACLNDCLDLYSDAIPTLKQAMIDYKSKHYKDANIEVSSVIDAATTC 146

Query: 153 EDGFKERKGVASPLKKRDGDAFELGAIALSIMSLL 188
           EDGF++++G  SPL KR+ D F+L AIAL+++++L
Sbjct: 147 EDGFEDKEGAVSPLTKRNNDTFQLSAIALALINML 180

The following BLAST results are available for this feature:

BLAST of CSPI06G06320 vs. Swiss-Prot


Match Name	E-value	Identity	Description
PLA1_PLAAC	5.7e-31	40.22	Putative invertase inhibitor OS=Platanus acerifolia PE=1 SV=1	[more]
CVIF2_ARATH	1.5e-07	26.67	Cell wall / vacuolar inhibitor of fructosidase 2 OS=Arabidopsis thaliana GN=C/VI...	[more]
PMEI_ACTDE	3.4e-07	29.10	Pectinesterase inhibitor OS=Actinidia deliciosa GN=PMEI PE=1 SV=2	[more]

BLAST of CSPI06G06320 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K951_CUCSA	2.0e-99	98.94	Uncharacterized protein OS=Cucumis sativus GN=Csa_6G080380 PE=4 SV=1	[more]
A0A0A0KBR8_CUCSA	2.8e-48	56.32	Uncharacterized protein OS=Cucumis sativus GN=Csa_6G080370 PE=4 SV=1	[more]
A0A0D2PD74_GOSRA	1.4e-47	56.18	Uncharacterized protein OS=Gossypium raimondii GN=B456_004G168200 PE=4 SV=1	[more]
B9MVC0_POPTR	8.9e-47	56.77	Invertase/pectin methylesterase inhibitor family protein OS=Populus trichocarpa ...	[more]
E5GC99_CUCME	9.8e-46	58.99	Invertase inhibitor OS=Cucumis melo subsp. melo PE=4 SV=1	[more]

BLAST of CSPI06G06320 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G46940.1	8.3e-20	31.82	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G46970.1	5.9e-18	33.33	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G38610.1	2.2e-17	33.33	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G46930.1	7.2e-16	30.68	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G46950.1	2.0e-13	29.78	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]

BLAST of CSPI06G06320 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449454568\|ref\|XP_004145026.1\|	2.9e-99	98.94	PREDICTED: putative invertase inhibitor [Cucumis sativus]	[more]
gi\|659120165\|ref\|XP_008460048.1\|	3.2e-90	90.91	PREDICTED: putative invertase inhibitor [Cucumis melo]	[more]
gi\|449454566\|ref\|XP_004145025.1\|	4.0e-48	56.32	PREDICTED: putative invertase inhibitor [Cucumis sativus]	[more]
gi\|823151115\|ref\|XP_012475386.1\|	2.0e-47	56.18	PREDICTED: putative invertase inhibitor [Gossypium raimondii]	[more]
gi\|566202453\|ref\|XP_006375099.1\|	1.3e-46	56.77	invertase/pectin methylesterase inhibitor family protein [Populus trichocarpa]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR006501	Pectinesterase_inhib_dom

Vocabulary: **Molecular Function**
Term	Definition
GO:0004857	enzyme inhibitor activity

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
biological_process	GO:0005982	starch metabolic process
biological_process	GO:0005985	sucrose metabolic process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004857	enzyme inhibitor activity
molecular_function	GO:0030599	pectinesterase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI06G06320.1	CSPI06G06320.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	GENE3D	G3DSA:1.20.140.40		coord: 32..187 score: 3.2
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 34..183 score: 1.5
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 30..183 score: 3.0
IPR006501	Pectinesterase inhibitor domain	TIGRFAMs	TIGR01614	TIGR01614	coord: 10..185 score: 1.6
IPR006501	Pectinesterase inhibitor domain	unknown	SSF101148	Plant invertase/pectin methylesterase inhibitor	coord: 32..187 score: 1.44
None	No IPR available	PANTHER	PTHR31707	FAMILY NOT NAMED	coord: 1..187 score: 1.2
None	No IPR available	PANTHER	PTHR31707:SF53	SUBFAMILY NOT NAMED	coord: 1..187 score: 1.2

Synteny

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CSPI06G06320	Cucumber (Chinese Long) v3	cpicucB335
CSPI06G06320	Cucumber (Chinese Long) v3	cpicucB364
CSPI06G06320	Watermelon (97103) v2	cpiwmbB485
CSPI06G06320	Watermelon (97103) v2	cpiwmbB495
CSPI06G06320	Wax gourd	cpiwgoB548
CSPI06G06320	Wax gourd	cpiwgoB560
CSPI06G06320	Wild cucumber (PI 183967)	cpicpiB126
CSPI06G06320	Cucumber (Gy14) v1	cgycpiB393
CSPI06G06320	Cucumber (Gy14) v1	cgycpiB540
CSPI06G06320	Cucurbita maxima (Rimu)	cmacpiB373
CSPI06G06320	Cucurbita maxima (Rimu)	cmacpiB856
CSPI06G06320	Cucurbita maxima (Rimu)	cmacpiB926
CSPI06G06320	Cucurbita moschata (Rifu)	cmocpiB362
CSPI06G06320	Cucurbita moschata (Rifu)	cmocpiB839
CSPI06G06320	Cucurbita moschata (Rifu)	cmocpiB905
CSPI06G06320	Cucumber (Chinese Long) v2	cpicuB283
CSPI06G06320	Cucumber (Chinese Long) v2	cpicuB314
CSPI06G06320	Melon (DHL92) v3.5.1	cpimeB433
CSPI06G06320	Melon (DHL92) v3.5.1	cpimeB496
CSPI06G06320	Watermelon (Charleston Gray)	cpiwcgB494
CSPI06G06320	Watermelon (Charleston Gray)	cpiwcgB503
CSPI06G06320	Watermelon (97103) v1	cpiwmB508
CSPI06G06320	Watermelon (97103) v1	cpiwmB556
CSPI06G06320	Cucurbita pepo (Zucchini)	cpecpiB145
CSPI06G06320	Cucurbita pepo (Zucchini)	cpecpiB318
CSPI06G06320	Cucurbita pepo (Zucchini)	cpecpiB879
CSPI06G06320	Bottle gourd (USVL1VR-Ls)	cpilsiB404
CSPI06G06320	Bottle gourd (USVL1VR-Ls)	cpilsiB470
CSPI06G06320	Melon (DHL92) v3.6.1	cpimedB431
CSPI06G06320	Melon (DHL92) v3.6.1	cpimedB483
CSPI06G06320	Cucumber (Gy14) v2	cgybcpiB141
CSPI06G06320	Cucumber (Gy14) v2	cgybcpiB288
CSPI06G06320	Silver-seed gourd	carcpiB0263
CSPI06G06320	Silver-seed gourd	carcpiB0999
CSPI06G06320	Silver-seed gourd	carcpiB1117