CSPI05G30470 (gene) Wild cucumber (PI 183967)

NameCSPI05G30470
Typegene
OrganismCucumis sativus (Wild cucumber (PI 183967))
DescriptionAcetyl esterase
LocationChr5 : 28366422 .. 28367572 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTTCGTCCAGCGTTTGTTACGAACTCTCGACTCCACAGCTTCCATGGAAGCACAAGGTCACTCTTCGATTCGCCACTCTACTCTTCAACACTTCTCTCCGATCAGATTTCACCGTCAATCGCCGGCTTCTCACTTTCCTCGACCCCAAAATCCCTCCCAATCCCAATTCAGCTCACTCCGTTTCCTCTTCCGATCTCACTATCGACACTTCTCGTGACCTCTTCCTTCGAATATTCACTCCCAACCCCACCGCCGCTCTCGATGAATCTCTCCCACTGCTGCCGATCATTTTCTACTTCCATGGCGGCGGCTTCGCCTTCGGTTCTGCCGACGCCACTTCAACTGACATGGCTGCCCGCGGCTTCGCCGAAAAGTTACGCGCCGTCGTAATCTCCGTCAACTACCGCCTCGCTCCTGAGTTTCGATTCCCATGCCAATATGACGATGGGTTCGACGCTTTGAAATTCATTGATGAAATGGACGACGACAGTTTACTAGAGAGGGTAGATTTGAGTCGTTGCTTTATTTTAGGGGAAAGCGCCGGTGGAAATCTAGGACACCATGTGGCAGTGAGAGCGAGCGAATACGAGTTCAAAAGAGTGAAGATCATTGGATTTATAGCGTCGCAGCCGTTTTTCGGCGGAAAGGAAAGAACGGAGTCAGAGAATCGACTGTGTAAGCAACTTCCGTTGACTCTATACATGACGGATTGGTTTTGGAGGGCATTTCTGCCAGCCGGGGAAGATCGAGACCATGCGGGGGCGAATATGAATGGACCCAATGGAAGGGATATCTCGGGGTTGGAAAATTTTCCGGCGACAGTGATTTTCGCGGGAGGATTGGATCTGCTGATGGATCGACAAAAAAGTTATTACGAGAGGTTGAAGAGAATGGGAAAGGATGTGAAGCTGGTGGTGTTTTCCAACGCCTTCCATGGATTCTTTGGCTTCCCAGATCTGCCGGAATATTCTTTGATGATTGAGGAGATGAGTGATTTCATTGCAAAGCTCATGTAGGAAAAATGAAGAATTAACATGTCGTGTGATATCTCTGTCTCCATTCTCATTGTTTATGCTCAAAATCAAAAAGTTGAAGGGACACCATTTCCAATAAAAAATAATCTCTACATCAACAAATTCTCTTAGCA

mRNA sequence

ATGGGTTCGTCCAGCGTTTGTTACGAACTCTCGACTCCACAGCTTCCATGGAAGCACAAGGTCACTCTTCGATTCGCCACTCTACTCTTCAACACTTCTCTCCGATCAGATTTCACCGTCAATCGCCGGCTTCTCACTTTCCTCGACCCCAAAATCCCTCCCAATCCCAATTCAGCTCACTCCGTTTCCTCTTCCGATCTCACTATCGACACTTCTCGTGACCTCTTCCTTCGAATATTCACTCCCAACCCCACCGCCGCTCTCGATGAATCTCTCCCACTGCTGCCGATCATTTTCTACTTCCATGGCGGCGGCTTCGCCTTCGGTTCTGCCGACGCCACTTCAACTGACATGGCTGCCCGCGGCTTCGCCGAAAAGTTACGCGCCGTCGTAATCTCCGTCAACTACCGCCTCGCTCCTGAGTTTCGATTCCCATGCCAATATGACGATGGGTTCGACGCTTTGAAATTCATTGATGAAATGGACGACGACAGTTTACTAGAGAGGGTAGATTTGAGTCGTTGCTTTATTTTAGGGGAAAGCGCCGGTGGAAATCTAGGACACCATGTGGCAGTGAGAGCGAGCGAATACGAGTTCAAAAGAGTGAAGATCATTGGATTTATAGCGTCGCAGCCGTTTTTCGGCGGAAAGGAAAGAACGGAGTCAGAGAATCGACTGTGTAAGCAACTTCCGTTGACTCTATACATGACGGATTGGTTTTGGAGGGCATTTCTGCCAGCCGGGGAAGATCGAGACCATGCGGGGGCGAATATGAATGGACCCAATGGAAGGGATATCTCGGGGTTGGAAAATTTTCCGGCGACAGTGATTTTCGCGGGAGGATTGGATCTGCTGATGGATCGACAAAAAAGTTATTACGAGAGGTTGAAGAGAATGGGAAAGGATGTGAAGCTGGTGGTGTTTTCCAACGCCTTCCATGGATTCTTTGGCTTCCCAGATCTGCCGGAATATTCTTTGATGATTGAGGAGATGAGTGATTTCATTGCAAAGCTCATGTAG

Coding sequence (CDS)

ATGGGTTCGTCCAGCGTTTGTTACGAACTCTCGACTCCACAGCTTCCATGGAAGCACAAGGTCACTCTTCGATTCGCCACTCTACTCTTCAACACTTCTCTCCGATCAGATTTCACCGTCAATCGCCGGCTTCTCACTTTCCTCGACCCCAAAATCCCTCCCAATCCCAATTCAGCTCACTCCGTTTCCTCTTCCGATCTCACTATCGACACTTCTCGTGACCTCTTCCTTCGAATATTCACTCCCAACCCCACCGCCGCTCTCGATGAATCTCTCCCACTGCTGCCGATCATTTTCTACTTCCATGGCGGCGGCTTCGCCTTCGGTTCTGCCGACGCCACTTCAACTGACATGGCTGCCCGCGGCTTCGCCGAAAAGTTACGCGCCGTCGTAATCTCCGTCAACTACCGCCTCGCTCCTGAGTTTCGATTCCCATGCCAATATGACGATGGGTTCGACGCTTTGAAATTCATTGATGAAATGGACGACGACAGTTTACTAGAGAGGGTAGATTTGAGTCGTTGCTTTATTTTAGGGGAAAGCGCCGGTGGAAATCTAGGACACCATGTGGCAGTGAGAGCGAGCGAATACGAGTTCAAAAGAGTGAAGATCATTGGATTTATAGCGTCGCAGCCGTTTTTCGGCGGAAAGGAAAGAACGGAGTCAGAGAATCGACTGTGTAAGCAACTTCCGTTGACTCTATACATGACGGATTGGTTTTGGAGGGCATTTCTGCCAGCCGGGGAAGATCGAGACCATGCGGGGGCGAATATGAATGGACCCAATGGAAGGGATATCTCGGGGTTGGAAAATTTTCCGGCGACAGTGATTTTCGCGGGAGGATTGGATCTGCTGATGGATCGACAAAAAAGTTATTACGAGAGGTTGAAGAGAATGGGAAAGGATGTGAAGCTGGTGGTGTTTTCCAACGCCTTCCATGGATTCTTTGGCTTCCCAGATCTGCCGGAATATTCTTTGATGATTGAGGAGATGAGTGATTTCATTGCAAAGCTCATGTAG
BLAST of CSPI05G30470 vs. Swiss-Prot
Match: CXE18_ARATH (Probable carboxylesterase 18 OS=Arabidopsis thaliana GN=CXE18 PE=1 SV=1)

HSP 1 Score: 295.0 bits (754), Expect = 1.0e-78
Identity = 152/324 (46.91%), Postives = 202/324 (62.35%), Query Frame = 1

Query: 15  LPWKHKVTLRFATLLFNTSLRSDFTVNRRLLTFLDPKIPPNPNSAHSVSSSDLTIDTSRD 74
           LP K ++ L   + + + + R D T+NRR L   D + PPNP   + VS+SD  +D SRD
Sbjct: 13  LPLKTRIALTVISTMTDNAQRPDGTINRRFLRLFDFRAPPNPKPVNIVSTSDFVVDQSRD 72

Query: 75  LFLRIFTPNPTAALDESLPLLPIIFYFHGGGFAFGSADATSTDMAARGFAEKLRAVVISV 134
           L+ R++TP+ +         +P++ +FHGGGFAF S +A   D   R FA KL A VISV
Sbjct: 73  LWFRLYTPHVSG------DKIPVVVFFHGGGFAFLSPNAYPYDNVCRRFARKLPAYVISV 132

Query: 135 NYRLAPEFRFPCQYDDGFDALKFIDEMDDDSLLERVDLSRCFILGESAGGNLGHHVAV-- 194
           NYRLAPE R+P QYDDGFDALK+I+E     L    DLSRCF  G+SAGGN+ H+VA+  
Sbjct: 133 NYRLAPEHRYPAQYDDGFDALKYIEENHGSILPANADLSRCFFAGDSAGGNIAHNVAIRI 192

Query: 195 -RASEYEFKRVKIIGFIASQPFFGGKERTESENRLCKQLPLTLYMTDWFWRAFLPAGEDR 254
            R     F  VK+IG I+ QPFFGG+ERTE+E +L     ++   TDW W+A    G +R
Sbjct: 193 CREPRSSFTAVKLIGLISIQPFFGGEERTEAEKQLVGAPLVSPDRTDWCWKAM---GLNR 252

Query: 255 DHAGANMNGPNGRDISGLENFPATVIFAGGLDLLMDRQKSYYERLKRMGKDVKLVVFSNA 314
           DH   N+ GPN  DISGL+ +P T++   G D L D Q+SYYE LK  GK   L+ + N 
Sbjct: 253 DHEAVNVGGPNAVDISGLD-YPETMVVVAGFDPLKDWQRSYYEWLKLCGKKATLIEYPNM 312

Query: 315 FHGFFGFPDLPEYSLMIEEMSDFI 336
           FH F+ FP+LPE   +I  + DF+
Sbjct: 313 FHAFYIFPELPEAGQLIMRIKDFV 326

BLAST of CSPI05G30470 vs. Swiss-Prot
Match: GID1C_ARATH (Gibberellin receptor GID1C OS=Arabidopsis thaliana GN=GID1C PE=1 SV=1)

HSP 1 Score: 234.6 bits (597), Expect = 1.6e-60
Identity = 134/343 (39.07%), Postives = 191/343 (55.69%), Query Frame = 1

Query: 2   GSSSVCYELSTPQLPWKHKVTLRFATLLFNTSLRSDFTVNRRLLTFLDPKIPPNPNSAHS 61
           GS  V    S   +P    V +    L +N   R D T NR L  FLD K+P N N  + 
Sbjct: 3   GSEEVNLIESKTVVPLNTWVLISNFKLAYNLLRRPDGTFNRHLAEFLDRKVPANANPVNG 62

Query: 62  VSSSDLTIDTSRDLFLRIFTP----NPTAALDESLP----LLPIIFYFHGGGFAFGSADA 121
           V S D+ ID   +L  R++ P       +  D   P    ++P+I +FHGG FA  SA++
Sbjct: 63  VFSFDVIIDRQTNLLSRVYRPADAGTSPSITDLQNPVDGEIVPVIVFFHGGSFAHSSANS 122

Query: 122 TSTDMAARGFAEKLRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDDSLLERVDLS 181
              D   R       AVV+SVNYR APE R+PC YDDG+  LK+++      L  + D  
Sbjct: 123 AIYDTLCRRLVGLCGAVVVSVNYRRAPENRYPCAYDDGWAVLKWVNS--SSWLRSKKDSK 182

Query: 182 -RCFILGESAGGNLGHHVAVRASEYEFKRVKIIGFIASQPFFGGKERTESENRLCKQLPL 241
            R F+ G+S+GGN+ H+VAVRA E    R+ ++G I   P FGG ERTESE RL  +  +
Sbjct: 183 VRIFLAGDSSGGNIVHNVAVRAVE---SRIDVLGNILLNPMFGGTERTESEKRLDGKYFV 242

Query: 242 TLYMTDWFWRAFLPAGEDRDHAGANMNGPNGRDISGLENFPATVIFAGGLDLLMDRQKSY 301
           T+   DW+WRAFLP GEDR+H   +  GP  + + GL +FP +++   GLDL+ D Q  Y
Sbjct: 243 TVRDRDWYWRAFLPEGEDREHPACSPFGPRSKSLEGL-SFPKSLVVVAGLDLIQDWQLKY 302

Query: 302 YERLKRMGKDVKLVVFSNAFHGFFGFPDLPEYSLMIEEMSDFI 336
            E LK+ G++VKL+    A  GF+  P+   +  +++E++ F+
Sbjct: 303 AEGLKKAGQEVKLLYLEQATIGFYLLPNNNHFHTVMDEIAAFV 339

BLAST of CSPI05G30470 vs. Swiss-Prot
Match: GID1_ORYSJ (Gibberellin receptor GID1 OS=Oryza sativa subsp. japonica GN=GID1 PE=1 SV=1)

HSP 1 Score: 229.6 bits (584), Expect = 5.3e-59
Identity = 134/326 (41.10%), Postives = 181/326 (55.52%), Query Frame = 1

Query: 28  LLFNTSLRSDFTVNRRLLTFLDPKIPPNPNSAHSVSSSDLTIDTSRDLFLRIFTPNPTAA 87
           L +N   R+D T  R L  +LD ++P N      VSS D  ID S  L +RI+       
Sbjct: 29  LSYNILRRADGTFERDLGEYLDRRVPANARPLEGVSSFDHIIDQSVGLEVRIYRAAAEGD 88

Query: 88  LDESL-----PLL------------PIIFYFHGGGFAFGSADATSTDMAARGFAEKLRAV 147
            +E       P+L            P+I +FHGG F   SA +T  D   R F +  + V
Sbjct: 89  AEEGAAAVTRPILEFLTDAPAAEPFPVIIFFHGGSFVHSSASSTIYDSLCRRFVKLSKGV 148

Query: 148 VISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDDSLLERVDL-SRCFILGESAGGNLGHH 207
           V+SVNYR APE R+PC YDDG+ ALK++  M    +    D  +R F+ G+S+GGN+ HH
Sbjct: 149 VVSVNYRRAPEHRYPCAYDDGWTALKWV--MSQPFMRSGGDAQARVFLSGDSSGGNIAHH 208

Query: 208 VAVRASEYEFKRVKIIGFIASQPFFGGKERTESENRLCKQLPLTLYMTDWFWRAFLPAGE 267
           VAVRA++   + VK+ G I     FGG ERTESE RL  +  +TL   DW+W+A+LP   
Sbjct: 209 VAVRAAD---EGVKVCGNILLNAMFGGTERTESERRLDGKYFVTLQDRDWYWKAYLPEDA 268

Query: 268 DRDHAGANMNGPNGRDISGLENFPATVIFAGGLDLLMDRQKSYYERLKRMGKDVKLVVFS 327
           DRDH   N  GPNGR + GL  F  ++I   GLDL  DRQ +Y + L+  G  VK+V   
Sbjct: 269 DRDHPACNPFGPNGRRLGGLP-FAKSLIIVSGLDLTCDRQLAYADALREDGHHVKVVQCE 328

Query: 328 NAFHGFFGFPDLPEYSLMIEEMSDFI 336
           NA  GF+  P+   Y  ++EE+SDF+
Sbjct: 329 NATVGFYLLPNTVHYHEVMEEISDFL 348

BLAST of CSPI05G30470 vs. Swiss-Prot
Match: GID1A_ARATH (Gibberellin receptor GID1A OS=Arabidopsis thaliana GN=GID1A PE=1 SV=1)

HSP 1 Score: 220.7 bits (561), Expect = 2.4e-56
Identity = 121/317 (38.17%), Postives = 179/317 (56.47%), Query Frame = 1

Query: 30  FNTSLRSDFTVNRRLLTFLDPKIPPNPNSAHSVSSSDLTIDTSRDLFLRIFTP------N 89
           +N   R D T NR L  +LD K+  N N    V S D+ ID   +L  R++ P       
Sbjct: 31  YNILRRPDGTFNRHLAEYLDRKVTANANPVDGVFSFDVLIDRRINLLSRVYRPAYADQEQ 90

Query: 90  PTAALDESLP----LLPIIFYFHGGGFAFGSADATSTDMAARGFAEKLRAVVISVNYRLA 149
           P + LD   P    ++P+I +FHGG FA  SA++   D   R      + VV+SVNYR A
Sbjct: 91  PPSILDLEKPVDGDIVPVILFFHGGSFAHSSANSAIYDTLCRRLVGLCKCVVVSVNYRRA 150

Query: 150 PEFRFPCQYDDGFDALKFIDEMDDDSLLERVDLS-RCFILGESAGGNLGHHVAVRASEYE 209
           PE  +PC YDDG+ AL +++      L  + D     F+ G+S+GGN+ H+VA+RA E  
Sbjct: 151 PENPYPCAYDDGWIALNWVNSRS--WLKSKKDSKVHIFLAGDSSGGNIAHNVALRAGE-- 210

Query: 210 FKRVKIIGFIASQPFFGGKERTESENRLCKQLPLTLYMTDWFWRAFLPAGEDRDHAGANM 269
              + ++G I   P FGG ERTESE  L  +  +T+   DW+W+AFLP GEDR+H   N 
Sbjct: 211 -SGIDVLGNILLNPMFGGNERTESEKSLDGKYFVTVRDRDWYWKAFLPEGEDREHPACNP 270

Query: 270 NGPNGRDISGLENFPATVIFAGGLDLLMDRQKSYYERLKRMGKDVKLVVFSNAFHGFFGF 329
             P G+ + G+ +FP +++   GLDL+ D Q +Y E LK+ G++VKL+    A  GF+  
Sbjct: 271 FSPRGKSLEGV-SFPKSLVVVAGLDLIRDWQLAYAEGLKKAGQEVKLMHLEKATVGFYLL 330

Query: 330 PDLPEYSLMIEEMSDFI 336
           P+   +  +++E+S F+
Sbjct: 331 PNNNHFHNVMDEISAFV 341

BLAST of CSPI05G30470 vs. Swiss-Prot
Match: GID1B_ARATH (Gibberellin receptor GID1B OS=Arabidopsis thaliana GN=GID1B PE=1 SV=1)

HSP 1 Score: 211.1 bits (536), Expect = 1.9e-53
Identity = 118/324 (36.42%), Postives = 182/324 (56.17%), Query Frame = 1

Query: 28  LLFNTSLRSDFTVNRRLLTFLDPKIPPNPNSAHSVSSSDLTIDTSRDLFLRIFTP----- 87
           L +    R D + NR L  FLD K+P N      V S D  +D++ +L  RI+ P     
Sbjct: 29  LAYKVLRRPDGSFNRDLAEFLDRKVPANSFPLDGVFSFD-HVDSTTNLLTRIYQPASLLH 88

Query: 88  ---NPTAALDESLP---LLPIIFYFHGGGFAFGSADATSTDMAARGFAEKLRAVVISVNY 147
              + T  L + L    ++P++ +FHGG F   SA++   D   R        VV+SV+Y
Sbjct: 89  QTRHGTLELTKPLSTTEIVPVLIFFHGGSFTHSSANSAIYDTFCRRLVTICGVVVVSVDY 148

Query: 148 RLAPEFRFPCQYDDGFDALKFID-----EMDDDSLLERVDLSRCFILGESAGGNLGHHVA 207
           R +PE R+PC YDDG++AL ++      +   DS +        ++ G+S+GGN+ H+VA
Sbjct: 149 RRSPEHRYPCAYDDGWNALNWVKSRVWLQSGKDSNVY------VYLAGDSSGGNIAHNVA 208

Query: 208 VRASEYEFKRVKIIGFIASQPFFGGKERTESENRLCKQLPLTLYMTDWFWRAFLPAGEDR 267
           VRA+    + VK++G I   P FGG+ERT+SE  L  +  +T+   DW+WRA+LP GEDR
Sbjct: 209 VRATN---EGVKVLGNILLHPMFGGQERTQSEKTLDGKYFVTIQDRDWYWRAYLPEGEDR 268

Query: 268 DHAGANMNGPNGRDISGLENFPATVIFAGGLDLLMDRQKSYYERLKRMGKDVKLVVFSNA 327
           DH   N  GP G+ + G+ NFP +++   GLDL+ D Q +Y + LK+ G +V L+    A
Sbjct: 269 DHPACNPFGPRGQSLKGV-NFPKSLVVVAGLDLVQDWQLAYVDGLKKTGLEVNLLYLKQA 328

Query: 328 FHGFFGFPDLPEYSLMIEEMSDFI 336
             GF+  P+   +  ++EE++ F+
Sbjct: 329 TIGFYFLPNNDHFHCLMEELNKFV 341

BLAST of CSPI05G30470 vs. TrEMBL
Match: A0A0A0KXS8_CUCSA (Acetyl esterase OS=Cucumis sativus GN=Csa_5G647390 PE=4 SV=1)

HSP 1 Score: 689.1 bits (1777), Expect = 2.7e-195
Identity = 336/339 (99.12%), Postives = 337/339 (99.41%), Query Frame = 1

Query: 1   MGSSSVCYELSTPQLPWKHKVTLRFATLLFNTSLRSDFTVNRRLLTFLDPKIPPNPNSAH 60
           M SSSVCYELSTPQLPWKHKVTLRFATLLFNTSLRSDFTVNRRLLTFLDPKIPPNPNSAH
Sbjct: 1   MRSSSVCYELSTPQLPWKHKVTLRFATLLFNTSLRSDFTVNRRLLTFLDPKIPPNPNSAH 60

Query: 61  SVSSSDLTIDTSRDLFLRIFTPNPTAALDESLPLLPIIFYFHGGGFAFGSADATSTDMAA 120
           SVSSSDLTIDTSRDLFLRIFTPNPTAALDESLPLLPIIFYFHGGGFAFGSADATSTDMAA
Sbjct: 61  SVSSSDLTIDTSRDLFLRIFTPNPTAALDESLPLLPIIFYFHGGGFAFGSADATSTDMAA 120

Query: 121 RGFAEKLRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDDSLLERVDLSRCFILGE 180
           RGFAEKLRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDDSLLERVDLSRCFILGE
Sbjct: 121 RGFAEKLRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDDSLLERVDLSRCFILGE 180

Query: 181 SAGGNLGHHVAVRASEYEFKRVKIIGFIASQPFFGGKERTESENRLCKQLPLTLYMTDWF 240
           SAGGNLGHHVAVRASEYEFKRVKIIGFIASQPFFGGKERTESENRLCKQLPLTLYMTDWF
Sbjct: 181 SAGGNLGHHVAVRASEYEFKRVKIIGFIASQPFFGGKERTESENRLCKQLPLTLYMTDWF 240

Query: 241 WRAFLPAGEDRDHAGANMNGPNGRDISGLENFPATVIFAGGLDLLMDRQKSYYERLKRMG 300
           WRAFLPAGEDRDHA AN+NGPNGRDISGLENFPATVIFAGGLDLLMDRQKSYYERLKRMG
Sbjct: 241 WRAFLPAGEDRDHAAANVNGPNGRDISGLENFPATVIFAGGLDLLMDRQKSYYERLKRMG 300

Query: 301 KDVKLVVFSNAFHGFFGFPDLPEYSLMIEEMSDFIAKLM 340
           KDVKLVVFSNAFHGFFGFPDLPEYSLMIEEMSDFIAKLM
Sbjct: 301 KDVKLVVFSNAFHGFFGFPDLPEYSLMIEEMSDFIAKLM 339

BLAST of CSPI05G30470 vs. TrEMBL
Match: A0A0A0KUP3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G647370 PE=4 SV=1)

HSP 1 Score: 433.7 bits (1114), Expect = 2.0e-118
Identity = 210/336 (62.50%), Postives = 256/336 (76.19%), Query Frame = 1

Query: 1   MGSSSVCYELSTPQLPWKHKVTLRFATLLFNTSLRSDFTVNRRLLTFLDPKIPPNPNSAH 60
           M SS+   E+S+ +LPWKH++ LR  + + +   RSDFTVNR     LD KIPP+     
Sbjct: 1   MSSSTSASEISSSKLPWKHRLLLRIGSTITDACCRSDFTVNRWFTGILDWKIPPSTKPID 60

Query: 61  SVSSSDLTIDTSRDLFLRIFTPNPTAALDESLPLLPIIFYFHGGGFAFGSADATSTDMAA 120
            VSS DLTIDTSR+L++RIF P      D  +  LP+IFYFHGGGFAF  AD+  +  +A
Sbjct: 61  GVSSFDLTIDTSRNLWVRIFNPVIDGE-DSDIQSLPLIFYFHGGGFAFSYADSALSHTSA 120

Query: 121 RGFAEKLRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDDSLLERVDLSRCFILGE 180
             FA++L AVVISVNYRLAPEFR+PCQYDDGFDALKFIDE+ ++ L  + DL+RCFILGE
Sbjct: 121 HRFAKQLPAVVISVNYRLAPEFRYPCQYDDGFDALKFIDEVGEEILPAKADLTRCFILGE 180

Query: 181 SAGGNLGHHVAVRASEYEFKRVKIIGFIASQPFFGGKERTESENRLCKQLPLTLYMTDWF 240
           SAGGNLGHHVAVRASEY  K+VK++GFIASQPFFGG+ERTESE RL  Q PL+L ++DWF
Sbjct: 181 SAGGNLGHHVAVRASEYTLKKVKMVGFIASQPFFGGEERTESEIRLSNQRPLSLRLSDWF 240

Query: 241 WRAFLPAGEDRDHAGANMNGPNGRDISGLENFPATVIFAGGLDLLMDRQKSYYERLKRMG 300
           W+AFLP GEDRDH  AN+ GP GRD++ +  FPAT++  G LDLL D Q+ YYE LKRMG
Sbjct: 241 WKAFLPEGEDRDHGAANVFGPKGRDVTEVMKFPATLVMVGELDLLQDGQRRYYEGLKRMG 300

Query: 301 KDVKLVVFSNAFHGFFGFPDLPEYSLMIEEMSDFIA 337
           K+VK+V F NA HGFF F DLP+YS M++EM DFIA
Sbjct: 301 KEVKMVEFENAIHGFFAFWDLPQYSSMMKEMKDFIA 335

BLAST of CSPI05G30470 vs. TrEMBL
Match: V4UJX3_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10026049mg PE=4 SV=1)

HSP 1 Score: 347.1 bits (889), Expect = 2.5e-92
Identity = 165/325 (50.77%), Postives = 221/325 (68.00%), Query Frame = 1

Query: 13  PQLPWKHKVTLRFATLLFNTSLRSDFTVNRRLLTFLDPKIPPNPNSAHSVSSSDLTIDTS 72
           P +PW  ++ L F + + ++  R D T+NRRL+ FLD K  PNPN  +SVS+SD+T+D S
Sbjct: 9   PAIPWTTRLALTFVSAIGDSGRRPDGTINRRLMNFLDVKSRPNPNPVNSVSTSDVTVDPS 68

Query: 73  RDLFLRIFTPNPTAALDESLPLLPIIFYFHGGGFAFGSADATSTDMAARGFAEKLRAVVI 132
           R L+ R+FTP       +S P LP++ +FHGGGF + SA + S D   R FA K  A V+
Sbjct: 69  RPLWFRLFTPT------DSTPSLPVLIFFHGGGFTYLSAASKSYDAVCRRFARKFPAFVV 128

Query: 133 SVNYRLAPEFRFPCQYDDGFDALKFIDEMDDDSLLERVDLSRCFILGESAGGNLGHHVAV 192
           SVNYRL PE R+P QYDDGFD L+F+D+  D  L    DLSRCF+ G+SAG NL HHVA+
Sbjct: 129 SVNYRLCPEHRYPSQYDDGFDVLRFLDDHRDSVLPPNADLSRCFLAGDSAGANLAHHVAL 188

Query: 193 RASEYEFKRVKIIGFIASQPFFGGKERTESENRLCKQLPLTLYMTDWFWRAFLPAGEDRD 252
           RAS   F+ VK++G ++ QPFFGG+ERTESE RL     ++   TDW W+AFLP G +RD
Sbjct: 189 RASGSPFRFVKLLGLVSIQPFFGGEERTESEIRLSGSPLVSTARTDWIWKAFLPDGSNRD 248

Query: 253 HAGANMNGPNGRDISGLENFPATVIFAGGLDLLMDRQKSYYERLKRMGKDVKLVVFSNAF 312
           H  +N++GPN  D+SGLE +P TV+  GG D L D Q+ YYE LK+ GK+  L+ + N  
Sbjct: 249 HEASNVSGPNAVDVSGLE-YPDTVVVVGGFDPLQDWQRRYYEWLKKSGKEATLIEYPNMI 308

Query: 313 HGFFGFPDLPEYSLMIEEMSDFIAK 338
           H F+ FP+LPE S +I ++ DFI+K
Sbjct: 309 HAFYVFPELPEASQLISQIKDFISK 326

BLAST of CSPI05G30470 vs. TrEMBL
Match: M5WIW5_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa019404mg PE=4 SV=1)

HSP 1 Score: 344.4 bits (882), Expect = 1.6e-91
Identity = 167/333 (50.15%), Postives = 229/333 (68.77%), Query Frame = 1

Query: 10  LSTPQLPWKHKVTLRFATLLFNTSLRSDFTVNRRLLTFLDPKIPPNPNSAHSVSSSDLTI 69
           +S P LP K ++ L   T++ NT  RS+ T+NRRL++  D K  P+    + V +SD+ +
Sbjct: 1   MSPPDLPCKVRLLLSLLTIVVNTVRRSNGTINRRLMSLFDLKASPSSKPNNRVKTSDVMV 60

Query: 70  DTSRDLFLRIFTPNPTAALDESLPL--LPIIFYFHGGGFAFGSADATSTDMAARGFAEKL 129
           D +R+L+ R++ P        + P   LP+I YFHGGGFAF SA++   D   +  + +L
Sbjct: 61  DPTRNLWFRLYVPAAAITTTTTSPSAKLPLIIYFHGGGFAFFSANSKPYDDLCKRLSAEL 120

Query: 130 RAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDDSL-LERVDLSRCFILGESAGGNL 189
            AVV+SVNYRLAPE  +P QY+DGFD LKFID+   D   L  VD++RCF+ G+SAGGNL
Sbjct: 121 PAVVVSVNYRLAPEHIYPSQYEDGFDVLKFIDQTTIDGFDLNNVDITRCFLAGDSAGGNL 180

Query: 190 GHHVAVRASEY-EFKRVKIIGFIASQPFFGGKERTESENRLCKQLPLTLYMTDWFWRAFL 249
            HHVAV+AS + EF +++++G IA QPFFGGKERTESE RL K   L+L  TDW+W+ FL
Sbjct: 181 AHHVAVKASNHHEFGKMRVVGLIAIQPFFGGKERTESETRLSKGSALSLEQTDWYWKTFL 240

Query: 250 PAGEDRDHAGANMNGPNGRDISGLENFPATVIFAGGLDLLMDRQKSYYERLKRMGKDVKL 309
           P G DRDHA AN+ G   +D+ G+ NFPAT++F GG DLL D Q+ YYE LK+ GK+  L
Sbjct: 241 PEGSDRDHAAANVFGQESKDVLGV-NFPATIVFVGGFDLLQDWQRRYYEGLKKSGKEAYL 300

Query: 310 VVFSNAFHGFFGFPDLPEYSLMIEEMSDFIAKL 339
           + + N+FHGF+ FP+L E S +++E+ DFI KL
Sbjct: 301 IEYPNSFHGFYAFPELKEPSFLVKEVRDFIEKL 332

BLAST of CSPI05G30470 vs. TrEMBL
Match: A0A0A0LMQ6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G401430 PE=4 SV=1)

HSP 1 Score: 343.2 bits (879), Expect = 3.6e-91
Identity = 175/330 (53.03%), Postives = 227/330 (68.79%), Query Frame = 1

Query: 11  STPQLPWKHKVTLRFATLLFNTSLRSDFTVNRRLLTFLDPKIPPNPNSAHSVSSSDLTID 70
           S P LPWK K+   F + LF  + RSDFTVNRR L   D K+PP   ++  V++SD TID
Sbjct: 13  SLPTLPWKRKLADLFTSFLFRQAFRSDFTVNRRFLRLFDRKLPPF--TSRGVAASDATID 72

Query: 71  TSR-DLFLRIFTPNPTAALDESLPLLPIIFYFHGGGFAFGSADATSTDMAARGFAEKLRA 130
           +S  DL++R++ P   +  D     LP+I YFHGGGF +GSADA  TD   R FA ++ A
Sbjct: 73  SSTSDLWIRVYNPLTFSNSDP----LPVIIYFHGGGFVYGSADAPPTDTFCRDFAREIGA 132

Query: 131 VVISVNYRLAPEFRFPCQYDDGFDALKFIDE-MDDDSLLERVDLSRCFILGESAGGNLGH 190
           +VISVNYRLAPE RFP Q+DDGF  LK +D+    +++ E  DL RCFI GESAGGN+ H
Sbjct: 133 IVISVNYRLAPEDRFPSQFDDGFHVLKAMDKGAISETVPENADLRRCFIAGESAGGNIAH 192

Query: 191 HVAVRASEYEFKRVKIIGFIASQPFFGGKERTESENRLCKQLPLTLYMTDWFWRAFLPAG 250
           HV VRA+E EFKRVKI+G I  QPFFGG+ER +SE R  +   LTL MTDWFW+A+LP G
Sbjct: 193 HVTVRAAESEFKRVKIVGMILIQPFFGGEERRDSEIRFGRGYGLTLEMTDWFWKAWLPVG 252

Query: 251 EDRDHAGANMNGPNGRDISGLENFPATVIFAGGLDLLMDRQKSYYERLKRMGKDVKLVVF 310
            +RDH  AN+ G +   ISG++  PA ++  GGLDLL DR + Y E LK+ G++V++V +
Sbjct: 253 SNRDHTAANVVGSS---ISGVK-VPAALVVIGGLDLLRDRNREYVEWLKKSGQEVRVVEY 312

Query: 311 SNAFHGFFGFPDLPEYSLMIEEMSDFIAKL 339
            N  HGF G PDLPEYS++I++   FI K+
Sbjct: 313 PNGTHGFIGKPDLPEYSMLIQDAKQFINKI 332

BLAST of CSPI05G30470 vs. TAIR10
Match: AT5G23530.1 (AT5G23530.1 carboxyesterase 18)

HSP 1 Score: 295.0 bits (754), Expect = 5.7e-80
Identity = 152/324 (46.91%), Postives = 202/324 (62.35%), Query Frame = 1

Query: 15  LPWKHKVTLRFATLLFNTSLRSDFTVNRRLLTFLDPKIPPNPNSAHSVSSSDLTIDTSRD 74
           LP K ++ L   + + + + R D T+NRR L   D + PPNP   + VS+SD  +D SRD
Sbjct: 13  LPLKTRIALTVISTMTDNAQRPDGTINRRFLRLFDFRAPPNPKPVNIVSTSDFVVDQSRD 72

Query: 75  LFLRIFTPNPTAALDESLPLLPIIFYFHGGGFAFGSADATSTDMAARGFAEKLRAVVISV 134
           L+ R++TP+ +         +P++ +FHGGGFAF S +A   D   R FA KL A VISV
Sbjct: 73  LWFRLYTPHVSG------DKIPVVVFFHGGGFAFLSPNAYPYDNVCRRFARKLPAYVISV 132

Query: 135 NYRLAPEFRFPCQYDDGFDALKFIDEMDDDSLLERVDLSRCFILGESAGGNLGHHVAV-- 194
           NYRLAPE R+P QYDDGFDALK+I+E     L    DLSRCF  G+SAGGN+ H+VA+  
Sbjct: 133 NYRLAPEHRYPAQYDDGFDALKYIEENHGSILPANADLSRCFFAGDSAGGNIAHNVAIRI 192

Query: 195 -RASEYEFKRVKIIGFIASQPFFGGKERTESENRLCKQLPLTLYMTDWFWRAFLPAGEDR 254
            R     F  VK+IG I+ QPFFGG+ERTE+E +L     ++   TDW W+A    G +R
Sbjct: 193 CREPRSSFTAVKLIGLISIQPFFGGEERTEAEKQLVGAPLVSPDRTDWCWKAM---GLNR 252

Query: 255 DHAGANMNGPNGRDISGLENFPATVIFAGGLDLLMDRQKSYYERLKRMGKDVKLVVFSNA 314
           DH   N+ GPN  DISGL+ +P T++   G D L D Q+SYYE LK  GK   L+ + N 
Sbjct: 253 DHEAVNVGGPNAVDISGLD-YPETMVVVAGFDPLKDWQRSYYEWLKLCGKKATLIEYPNM 312

Query: 315 FHGFFGFPDLPEYSLMIEEMSDFI 336
           FH F+ FP+LPE   +I  + DF+
Sbjct: 313 FHAFYIFPELPEAGQLIMRIKDFV 326

BLAST of CSPI05G30470 vs. TAIR10
Match: AT5G27320.1 (AT5G27320.1 alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 234.6 bits (597), Expect = 9.2e-62
Identity = 134/343 (39.07%), Postives = 191/343 (55.69%), Query Frame = 1

Query: 2   GSSSVCYELSTPQLPWKHKVTLRFATLLFNTSLRSDFTVNRRLLTFLDPKIPPNPNSAHS 61
           GS  V    S   +P    V +    L +N   R D T NR L  FLD K+P N N  + 
Sbjct: 3   GSEEVNLIESKTVVPLNTWVLISNFKLAYNLLRRPDGTFNRHLAEFLDRKVPANANPVNG 62

Query: 62  VSSSDLTIDTSRDLFLRIFTP----NPTAALDESLP----LLPIIFYFHGGGFAFGSADA 121
           V S D+ ID   +L  R++ P       +  D   P    ++P+I +FHGG FA  SA++
Sbjct: 63  VFSFDVIIDRQTNLLSRVYRPADAGTSPSITDLQNPVDGEIVPVIVFFHGGSFAHSSANS 122

Query: 122 TSTDMAARGFAEKLRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDDSLLERVDLS 181
              D   R       AVV+SVNYR APE R+PC YDDG+  LK+++      L  + D  
Sbjct: 123 AIYDTLCRRLVGLCGAVVVSVNYRRAPENRYPCAYDDGWAVLKWVNS--SSWLRSKKDSK 182

Query: 182 -RCFILGESAGGNLGHHVAVRASEYEFKRVKIIGFIASQPFFGGKERTESENRLCKQLPL 241
            R F+ G+S+GGN+ H+VAVRA E    R+ ++G I   P FGG ERTESE RL  +  +
Sbjct: 183 VRIFLAGDSSGGNIVHNVAVRAVE---SRIDVLGNILLNPMFGGTERTESEKRLDGKYFV 242

Query: 242 TLYMTDWFWRAFLPAGEDRDHAGANMNGPNGRDISGLENFPATVIFAGGLDLLMDRQKSY 301
           T+   DW+WRAFLP GEDR+H   +  GP  + + GL +FP +++   GLDL+ D Q  Y
Sbjct: 243 TVRDRDWYWRAFLPEGEDREHPACSPFGPRSKSLEGL-SFPKSLVVVAGLDLIQDWQLKY 302

Query: 302 YERLKRMGKDVKLVVFSNAFHGFFGFPDLPEYSLMIEEMSDFI 336
            E LK+ G++VKL+    A  GF+  P+   +  +++E++ F+
Sbjct: 303 AEGLKKAGQEVKLLYLEQATIGFYLLPNNNHFHTVMDEIAAFV 339

BLAST of CSPI05G30470 vs. TAIR10
Match: AT3G05120.1 (AT3G05120.1 alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 220.7 bits (561), Expect = 1.4e-57
Identity = 121/317 (38.17%), Postives = 179/317 (56.47%), Query Frame = 1

Query: 30  FNTSLRSDFTVNRRLLTFLDPKIPPNPNSAHSVSSSDLTIDTSRDLFLRIFTP------N 89
           +N   R D T NR L  +LD K+  N N    V S D+ ID   +L  R++ P       
Sbjct: 31  YNILRRPDGTFNRHLAEYLDRKVTANANPVDGVFSFDVLIDRRINLLSRVYRPAYADQEQ 90

Query: 90  PTAALDESLP----LLPIIFYFHGGGFAFGSADATSTDMAARGFAEKLRAVVISVNYRLA 149
           P + LD   P    ++P+I +FHGG FA  SA++   D   R      + VV+SVNYR A
Sbjct: 91  PPSILDLEKPVDGDIVPVILFFHGGSFAHSSANSAIYDTLCRRLVGLCKCVVVSVNYRRA 150

Query: 150 PEFRFPCQYDDGFDALKFIDEMDDDSLLERVDLS-RCFILGESAGGNLGHHVAVRASEYE 209
           PE  +PC YDDG+ AL +++      L  + D     F+ G+S+GGN+ H+VA+RA E  
Sbjct: 151 PENPYPCAYDDGWIALNWVNSRS--WLKSKKDSKVHIFLAGDSSGGNIAHNVALRAGE-- 210

Query: 210 FKRVKIIGFIASQPFFGGKERTESENRLCKQLPLTLYMTDWFWRAFLPAGEDRDHAGANM 269
              + ++G I   P FGG ERTESE  L  +  +T+   DW+W+AFLP GEDR+H   N 
Sbjct: 211 -SGIDVLGNILLNPMFGGNERTESEKSLDGKYFVTVRDRDWYWKAFLPEGEDREHPACNP 270

Query: 270 NGPNGRDISGLENFPATVIFAGGLDLLMDRQKSYYERLKRMGKDVKLVVFSNAFHGFFGF 329
             P G+ + G+ +FP +++   GLDL+ D Q +Y E LK+ G++VKL+    A  GF+  
Sbjct: 271 FSPRGKSLEGV-SFPKSLVVVAGLDLIRDWQLAYAEGLKKAGQEVKLMHLEKATVGFYLL 330

Query: 330 PDLPEYSLMIEEMSDFI 336
           P+   +  +++E+S F+
Sbjct: 331 PNNNHFHNVMDEISAFV 341

BLAST of CSPI05G30470 vs. TAIR10
Match: AT3G63010.1 (AT3G63010.1 alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 211.1 bits (536), Expect = 1.1e-54
Identity = 118/324 (36.42%), Postives = 182/324 (56.17%), Query Frame = 1

Query: 28  LLFNTSLRSDFTVNRRLLTFLDPKIPPNPNSAHSVSSSDLTIDTSRDLFLRIFTP----- 87
           L +    R D + NR L  FLD K+P N      V S D  +D++ +L  RI+ P     
Sbjct: 29  LAYKVLRRPDGSFNRDLAEFLDRKVPANSFPLDGVFSFD-HVDSTTNLLTRIYQPASLLH 88

Query: 88  ---NPTAALDESLP---LLPIIFYFHGGGFAFGSADATSTDMAARGFAEKLRAVVISVNY 147
              + T  L + L    ++P++ +FHGG F   SA++   D   R        VV+SV+Y
Sbjct: 89  QTRHGTLELTKPLSTTEIVPVLIFFHGGSFTHSSANSAIYDTFCRRLVTICGVVVVSVDY 148

Query: 148 RLAPEFRFPCQYDDGFDALKFID-----EMDDDSLLERVDLSRCFILGESAGGNLGHHVA 207
           R +PE R+PC YDDG++AL ++      +   DS +        ++ G+S+GGN+ H+VA
Sbjct: 149 RRSPEHRYPCAYDDGWNALNWVKSRVWLQSGKDSNVY------VYLAGDSSGGNIAHNVA 208

Query: 208 VRASEYEFKRVKIIGFIASQPFFGGKERTESENRLCKQLPLTLYMTDWFWRAFLPAGEDR 267
           VRA+    + VK++G I   P FGG+ERT+SE  L  +  +T+   DW+WRA+LP GEDR
Sbjct: 209 VRATN---EGVKVLGNILLHPMFGGQERTQSEKTLDGKYFVTIQDRDWYWRAYLPEGEDR 268

Query: 268 DHAGANMNGPNGRDISGLENFPATVIFAGGLDLLMDRQKSYYERLKRMGKDVKLVVFSNA 327
           DH   N  GP G+ + G+ NFP +++   GLDL+ D Q +Y + LK+ G +V L+    A
Sbjct: 269 DHPACNPFGPRGQSLKGV-NFPKSLVVVAGLDLVQDWQLAYVDGLKKTGLEVNLLYLKQA 328

Query: 328 FHGFFGFPDLPEYSLMIEEMSDFI 336
             GF+  P+   +  ++EE++ F+
Sbjct: 329 TIGFYFLPNNDHFHCLMEELNKFV 341

BLAST of CSPI05G30470 vs. TAIR10
Match: AT2G45600.1 (AT2G45600.1 alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 168.7 bits (426), Expect = 6.2e-42
Identity = 104/295 (35.25%), Postives = 155/295 (52.54%), Query Frame = 1

Query: 31  NTSLRSDFTVNRRLLTFLDPKIPPNPNSAHSVSSSDLTIDTSRDLFLRIFTPNPTAALDE 90
           N +L SD ++ R       PK+PP   S       D+ ++ + + F+RIF P       +
Sbjct: 15  NITLNSDGSLTRHRDF---PKLPPTEQS------KDIPLNQTNNTFIRIFKPRNIPPESK 74

Query: 91  SLPLLPIIFYFHGGGFAFGSADATSTDMAARGFAEKLRAVVISVNYRLAPEFRFPCQYDD 150
               LPI+ YFHGGGF   SA +     +    A++L+ +++SV YRLAPE R P  Y+D
Sbjct: 75  ----LPILVYFHGGGFILYSAASAPFHESCTKMADRLQTIILSVEYRLAPEHRLPAAYED 134

Query: 151 GFDALKFIDEMD---------DDSLLERVDLSRCFILGESAGGNLGHHVAVRASEYEFKR 210
             +A+ ++ +           D  L + VD S+C+++G S+GGN+ ++VA+R  + +   
Sbjct: 135 AVEAILWLRDQARGPINGGDCDTWLKDGVDFSKCYVMGSSSGGNIVYNVALRVVDTDLSP 194

Query: 211 VKIIGFIASQPFFGGKERTESENRLCKQLPLTLYMTDWFWRAFLPAGEDRDHAGAN---M 270
           VKI G I +Q FFGG E ++SE+RL       L  T   W   LP G DRDH  +N    
Sbjct: 195 VKIQGLIMNQAFFGGVEPSDSESRLKDDKICPLPATHLLWSLCLPDGVDRDHVYSNPIKS 254

Query: 271 NGPNGRDISGLENFPATVIFAGGLDLLMDRQKSYYERLKRMGKDVKLVVFSNAFH 314
           +GP  +D  G   FP+T+I   G D L+DRQ+   E LK  G  V+     + FH
Sbjct: 255 SGPQEKDKMG--RFPSTLINGYGGDPLVDRQRHVAEMLKGRGVHVETRFDKDGFH 294

BLAST of CSPI05G30470 vs. NCBI nr
Match: gi|449447725|ref|XP_004141618.1| (PREDICTED: probable carboxylesterase 18 [Cucumis sativus])

HSP 1 Score: 689.1 bits (1777), Expect = 3.9e-195
Identity = 336/339 (99.12%), Postives = 337/339 (99.41%), Query Frame = 1

Query: 1   MGSSSVCYELSTPQLPWKHKVTLRFATLLFNTSLRSDFTVNRRLLTFLDPKIPPNPNSAH 60
           M SSSVCYELSTPQLPWKHKVTLRFATLLFNTSLRSDFTVNRRLLTFLDPKIPPNPNSAH
Sbjct: 1   MRSSSVCYELSTPQLPWKHKVTLRFATLLFNTSLRSDFTVNRRLLTFLDPKIPPNPNSAH 60

Query: 61  SVSSSDLTIDTSRDLFLRIFTPNPTAALDESLPLLPIIFYFHGGGFAFGSADATSTDMAA 120
           SVSSSDLTIDTSRDLFLRIFTPNPTAALDESLPLLPIIFYFHGGGFAFGSADATSTDMAA
Sbjct: 61  SVSSSDLTIDTSRDLFLRIFTPNPTAALDESLPLLPIIFYFHGGGFAFGSADATSTDMAA 120

Query: 121 RGFAEKLRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDDSLLERVDLSRCFILGE 180
           RGFAEKLRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDDSLLERVDLSRCFILGE
Sbjct: 121 RGFAEKLRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDDSLLERVDLSRCFILGE 180

Query: 181 SAGGNLGHHVAVRASEYEFKRVKIIGFIASQPFFGGKERTESENRLCKQLPLTLYMTDWF 240
           SAGGNLGHHVAVRASEYEFKRVKIIGFIASQPFFGGKERTESENRLCKQLPLTLYMTDWF
Sbjct: 181 SAGGNLGHHVAVRASEYEFKRVKIIGFIASQPFFGGKERTESENRLCKQLPLTLYMTDWF 240

Query: 241 WRAFLPAGEDRDHAGANMNGPNGRDISGLENFPATVIFAGGLDLLMDRQKSYYERLKRMG 300
           WRAFLPAGEDRDHA AN+NGPNGRDISGLENFPATVIFAGGLDLLMDRQKSYYERLKRMG
Sbjct: 241 WRAFLPAGEDRDHAAANVNGPNGRDISGLENFPATVIFAGGLDLLMDRQKSYYERLKRMG 300

Query: 301 KDVKLVVFSNAFHGFFGFPDLPEYSLMIEEMSDFIAKLM 340
           KDVKLVVFSNAFHGFFGFPDLPEYSLMIEEMSDFIAKLM
Sbjct: 301 KDVKLVVFSNAFHGFFGFPDLPEYSLMIEEMSDFIAKLM 339

BLAST of CSPI05G30470 vs. NCBI nr
Match: gi|659119543|ref|XP_008459713.1| (PREDICTED: probable carboxylesterase 18 [Cucumis melo])

HSP 1 Score: 630.9 bits (1626), Expect = 1.3e-177
Identity = 305/337 (90.50%), Postives = 319/337 (94.66%), Query Frame = 1

Query: 1   MGSSSVCYELSTPQLPWKHKVTLRFATLLFNTSLRSDFTVNRRLLTFLDPKIPPNPNSAH 60
           M SS VCY++STPQLPWKH++TLRFATLL+NTSLRSD TVNRRLL F+DPKIP N +S H
Sbjct: 1   MCSSGVCYQISTPQLPWKHRITLRFATLLYNTSLRSDLTVNRRLLNFIDPKIPSNHDSTH 60

Query: 61  SVSSSDLTIDTSRDLFLRIFTPNPTAALDESLPLLPIIFYFHGGGFAFGSADATSTDMAA 120
           SVSSSDL +DTSRDLFLRIFTP PTAALDESLPLLPIIFYFHGGGF FGSADATSTDMAA
Sbjct: 61  SVSSSDLIVDTSRDLFLRIFTPTPTAALDESLPLLPIIFYFHGGGFVFGSADATSTDMAA 120

Query: 121 RGFAEKLRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDDSLLERVDLSRCFILGE 180
           R FA+KLRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDDSLLERVDLS CFILGE
Sbjct: 121 RRFAKKLRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDDSLLERVDLSCCFILGE 180

Query: 181 SAGGNLGHHVAVRASEYEFKRVKIIGFIASQPFFGGKERTESENRLCKQLPLTLYMTDWF 240
           SAGGNLGHHVAVRASEYEFKRVKIIGFIASQPFFGGKERTESENRLCKQLPLTL MTDWF
Sbjct: 181 SAGGNLGHHVAVRASEYEFKRVKIIGFIASQPFFGGKERTESENRLCKQLPLTLDMTDWF 240

Query: 241 WRAFLPAGEDRDHAGANMNGPNGRDISGLENFPATVIFAGGLDLLMDRQKSYYERLKRMG 300
           WRAFLPAGEDRDHA AN+NGPNGRDISGLENFPAT+IFAGGLDLL+DRQK YYERLKRMG
Sbjct: 241 WRAFLPAGEDRDHAAANVNGPNGRDISGLENFPATLIFAGGLDLLIDRQKCYYERLKRMG 300

Query: 301 KDVKLVVFSNAFHGFFGFPDLPEYSLMIEEMSDFIAK 338
           KDVKLVVFSNA HG+FGF DLPEYSLMI+EM DFIAK
Sbjct: 301 KDVKLVVFSNAVHGYFGFADLPEYSLMIKEMRDFIAK 337

BLAST of CSPI05G30470 vs. NCBI nr
Match: gi|659119541|ref|XP_008459712.1| (PREDICTED: probable carboxylesterase 18 [Cucumis melo])

HSP 1 Score: 436.8 bits (1122), Expect = 3.5e-119
Identity = 216/337 (64.09%), Postives = 259/337 (76.85%), Query Frame = 1

Query: 1   MGSSSV-CYELSTPQLPWKHKVTLRFATLLFNTSLRSDFTVNRRLLTFLDPKIPPNPNSA 60
           M SSS+   E+S+ +LPWKH++ LR  T++ +   RSDFTVNR L   LD KIPP+    
Sbjct: 1   MSSSSISACEISSSKLPWKHRLLLRIGTIITDACCRSDFTVNRWLSGTLDWKIPPSTKPI 60

Query: 61  HSVSSSDLTIDTSRDLFLRIFTPNPTAALDESLPLLPIIFYFHGGGFAFGSADATSTDMA 120
             VSS DLTIDTSR+L++RIF P      D +L  LP+IFYFHGGGFAF  AD+  +  +
Sbjct: 61  DGVSSFDLTIDTSRNLWVRIFNPIIDDE-DSNLQSLPLIFYFHGGGFAFSYADSALSHTS 120

Query: 121 ARGFAEKLRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDDSLLERVDLSRCFILG 180
           A  FA++L AVVISVNYRLAPEFR+PCQYDDGFDALKFIDE+ ++ L  + DLSRCFILG
Sbjct: 121 AHRFAKQLPAVVISVNYRLAPEFRYPCQYDDGFDALKFIDEVGEEILPAKADLSRCFILG 180

Query: 181 ESAGGNLGHHVAVRASEYEFKRVKIIGFIASQPFFGGKERTESENRLCKQLPLTLYMTDW 240
           ESAGGNLGHHVAVRASEY  K+V+++GFIASQPFFGG+ERTESE RL  Q PL L ++DW
Sbjct: 181 ESAGGNLGHHVAVRASEYTLKKVRLVGFIASQPFFGGEERTESEIRLRNQRPLNLRLSDW 240

Query: 241 FWRAFLPAGEDRDHAGANMNGPNGRDISGLENFPATVIFAGGLDLLMDRQKSYYERLKRM 300
           FW+AFLP GEDRDHA AN+ GP GRD+  +  FPAT++  G LDLL D Q+ YYE LKRM
Sbjct: 241 FWKAFLPEGEDRDHAAANVFGPKGRDVREVMKFPATLVLVGELDLLQDGQRRYYEGLKRM 300

Query: 301 GKDVKLVVFSNAFHGFFGFPDLPEYSLMIEEMSDFIA 337
           GK+VK+V F NA HGFF F DLP+YS MI+EM DFIA
Sbjct: 301 GKEVKMVEFENAIHGFFAFWDLPQYSSMIKEMKDFIA 336

BLAST of CSPI05G30470 vs. NCBI nr
Match: gi|449447721|ref|XP_004141616.1| (PREDICTED: probable carboxylesterase 18 [Cucumis sativus])

HSP 1 Score: 433.7 bits (1114), Expect = 2.9e-118
Identity = 210/336 (62.50%), Postives = 256/336 (76.19%), Query Frame = 1

Query: 1   MGSSSVCYELSTPQLPWKHKVTLRFATLLFNTSLRSDFTVNRRLLTFLDPKIPPNPNSAH 60
           M SS+   E+S+ +LPWKH++ LR  + + +   RSDFTVNR     LD KIPP+     
Sbjct: 1   MSSSTSASEISSSKLPWKHRLLLRIGSTITDACCRSDFTVNRWFTGILDWKIPPSTKPID 60

Query: 61  SVSSSDLTIDTSRDLFLRIFTPNPTAALDESLPLLPIIFYFHGGGFAFGSADATSTDMAA 120
            VSS DLTIDTSR+L++RIF P      D  +  LP+IFYFHGGGFAF  AD+  +  +A
Sbjct: 61  GVSSFDLTIDTSRNLWVRIFNPVIDGE-DSDIQSLPLIFYFHGGGFAFSYADSALSHTSA 120

Query: 121 RGFAEKLRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDDSLLERVDLSRCFILGE 180
             FA++L AVVISVNYRLAPEFR+PCQYDDGFDALKFIDE+ ++ L  + DL+RCFILGE
Sbjct: 121 HRFAKQLPAVVISVNYRLAPEFRYPCQYDDGFDALKFIDEVGEEILPAKADLTRCFILGE 180

Query: 181 SAGGNLGHHVAVRASEYEFKRVKIIGFIASQPFFGGKERTESENRLCKQLPLTLYMTDWF 240
           SAGGNLGHHVAVRASEY  K+VK++GFIASQPFFGG+ERTESE RL  Q PL+L ++DWF
Sbjct: 181 SAGGNLGHHVAVRASEYTLKKVKMVGFIASQPFFGGEERTESEIRLSNQRPLSLRLSDWF 240

Query: 241 WRAFLPAGEDRDHAGANMNGPNGRDISGLENFPATVIFAGGLDLLMDRQKSYYERLKRMG 300
           W+AFLP GEDRDH  AN+ GP GRD++ +  FPAT++  G LDLL D Q+ YYE LKRMG
Sbjct: 241 WKAFLPEGEDRDHGAANVFGPKGRDVTEVMKFPATLVMVGELDLLQDGQRRYYEGLKRMG 300

Query: 301 KDVKLVVFSNAFHGFFGFPDLPEYSLMIEEMSDFIA 337
           K+VK+V F NA HGFF F DLP+YS M++EM DFIA
Sbjct: 301 KEVKMVEFENAIHGFFAFWDLPQYSSMMKEMKDFIA 335

BLAST of CSPI05G30470 vs. NCBI nr
Match: gi|567867523|ref|XP_006426384.1| (hypothetical protein CICLE_v10026049mg [Citrus clementina])

HSP 1 Score: 347.1 bits (889), Expect = 3.6e-92
Identity = 165/325 (50.77%), Postives = 221/325 (68.00%), Query Frame = 1

Query: 13  PQLPWKHKVTLRFATLLFNTSLRSDFTVNRRLLTFLDPKIPPNPNSAHSVSSSDLTIDTS 72
           P +PW  ++ L F + + ++  R D T+NRRL+ FLD K  PNPN  +SVS+SD+T+D S
Sbjct: 9   PAIPWTTRLALTFVSAIGDSGRRPDGTINRRLMNFLDVKSRPNPNPVNSVSTSDVTVDPS 68

Query: 73  RDLFLRIFTPNPTAALDESLPLLPIIFYFHGGGFAFGSADATSTDMAARGFAEKLRAVVI 132
           R L+ R+FTP       +S P LP++ +FHGGGF + SA + S D   R FA K  A V+
Sbjct: 69  RPLWFRLFTPT------DSTPSLPVLIFFHGGGFTYLSAASKSYDAVCRRFARKFPAFVV 128

Query: 133 SVNYRLAPEFRFPCQYDDGFDALKFIDEMDDDSLLERVDLSRCFILGESAGGNLGHHVAV 192
           SVNYRL PE R+P QYDDGFD L+F+D+  D  L    DLSRCF+ G+SAG NL HHVA+
Sbjct: 129 SVNYRLCPEHRYPSQYDDGFDVLRFLDDHRDSVLPPNADLSRCFLAGDSAGANLAHHVAL 188

Query: 193 RASEYEFKRVKIIGFIASQPFFGGKERTESENRLCKQLPLTLYMTDWFWRAFLPAGEDRD 252
           RAS   F+ VK++G ++ QPFFGG+ERTESE RL     ++   TDW W+AFLP G +RD
Sbjct: 189 RASGSPFRFVKLLGLVSIQPFFGGEERTESEIRLSGSPLVSTARTDWIWKAFLPDGSNRD 248

Query: 253 HAGANMNGPNGRDISGLENFPATVIFAGGLDLLMDRQKSYYERLKRMGKDVKLVVFSNAF 312
           H  +N++GPN  D+SGLE +P TV+  GG D L D Q+ YYE LK+ GK+  L+ + N  
Sbjct: 249 HEASNVSGPNAVDVSGLE-YPDTVVVVGGFDPLQDWQRRYYEWLKKSGKEATLIEYPNMI 308

Query: 313 HGFFGFPDLPEYSLMIEEMSDFIAK 338
           H F+ FP+LPE S +I ++ DFI+K
Sbjct: 309 HAFYVFPELPEASQLISQIKDFISK 326

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
CXE18_ARATH1.0e-7846.91Probable carboxylesterase 18 OS=Arabidopsis thaliana GN=CXE18 PE=1 SV=1[more]
GID1C_ARATH1.6e-6039.07Gibberellin receptor GID1C OS=Arabidopsis thaliana GN=GID1C PE=1 SV=1[more]
GID1_ORYSJ5.3e-5941.10Gibberellin receptor GID1 OS=Oryza sativa subsp. japonica GN=GID1 PE=1 SV=1[more]
GID1A_ARATH2.4e-5638.17Gibberellin receptor GID1A OS=Arabidopsis thaliana GN=GID1A PE=1 SV=1[more]
GID1B_ARATH1.9e-5336.42Gibberellin receptor GID1B OS=Arabidopsis thaliana GN=GID1B PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KXS8_CUCSA2.7e-19599.12Acetyl esterase OS=Cucumis sativus GN=Csa_5G647390 PE=4 SV=1[more]
A0A0A0KUP3_CUCSA2.0e-11862.50Uncharacterized protein OS=Cucumis sativus GN=Csa_5G647370 PE=4 SV=1[more]
V4UJX3_9ROSI2.5e-9250.77Uncharacterized protein OS=Citrus clementina GN=CICLE_v10026049mg PE=4 SV=1[more]
M5WIW5_PRUPE1.6e-9150.15Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa019404mg PE=4 SV=1[more]
A0A0A0LMQ6_CUCSA3.6e-9153.03Uncharacterized protein OS=Cucumis sativus GN=Csa_2G401430 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G23530.15.7e-8046.91 carboxyesterase 18[more]
AT5G27320.19.2e-6239.07 alpha/beta-Hydrolases superfamily protein[more]
AT3G05120.11.4e-5738.17 alpha/beta-Hydrolases superfamily protein[more]
AT3G63010.11.1e-5436.42 alpha/beta-Hydrolases superfamily protein[more]
AT2G45600.16.2e-4235.25 alpha/beta-Hydrolases superfamily protein[more]
Match NameE-valueIdentityDescription
gi|449447725|ref|XP_004141618.1|3.9e-19599.12PREDICTED: probable carboxylesterase 18 [Cucumis sativus][more]
gi|659119543|ref|XP_008459713.1|1.3e-17790.50PREDICTED: probable carboxylesterase 18 [Cucumis melo][more]
gi|659119541|ref|XP_008459712.1|3.5e-11964.09PREDICTED: probable carboxylesterase 18 [Cucumis melo][more]
gi|449447721|ref|XP_004141616.1|2.9e-11862.50PREDICTED: probable carboxylesterase 18 [Cucumis sativus][more]
gi|567867523|ref|XP_006426384.1|3.6e-9250.77hypothetical protein CICLE_v10026049mg [Citrus clementina][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR002168Lipase_GDXG_HIS_AS
IPR013094AB_hydrolase_3
Vocabulary: Biological Process
TermDefinition
GO:0008152metabolic process
Vocabulary: Molecular Function
TermDefinition
GO:0016787hydrolase activity
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008152 metabolic process
cellular_component GO:0005575 cellular_component
molecular_function GO:0016787 hydrolase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI05G30470.1CSPI05G30470.1mRNA


Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002168Lipase, GDXG, putative histidine active sitePROSITEPS01173LIPASE_GDXG_HIScoord: 98..114
scor
IPR013094Alpha/beta hydrolase fold-3PFAMPF07859Abhydrolase_3coord: 98..316
score: 2.7
NoneNo IPR availablePANTHERPTHR23024MEMBER OF 'GDXG' FAMILY OF LIPOLYTIC ENZYMEScoord: 34..339
score: 2.2E
NoneNo IPR availablePANTHERPTHR23024:SF174SUBFAMILY NOT NAMEDcoord: 34..339
score: 2.2E

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None