CSPI05G28500 (gene) Wild cucumber (PI 183967) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CSPI05G28500
Type	gene
Organism	Cucumis sativus (Wild cucumber (PI 183967))
Description	Matrix metalloproteinase
Location	Chr5 : 26887183 .. 26888465 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCATTCGAAACATTATTATCATTCTTAACGACCTCTTTTTTTCTTGTAATTGTCCTCTTCCCTTTGATATCATCTTCTCATCCAAAGAGTCATCTTTCCAATGGATTGGTGTTTCTAAAGAACCTCAATGGATGCAAGAAAGGTGACAAAGTAGAAGGAATTCACCAAGTGAAGGAATATCTTCAACATTTTGGCTATTTGAATAACTATGTTCAAAATTATTCTAAATCTTTTGATGATGATGAGTTCGATGGTGTTTTAGAGTCCGCCATTAAAACTTATCAACTCAACTACAATCTCAAGGCCACAGGTACTATCAATGCCAAGACGCTAGATCTTATGTCGAGACCTCGATGTGGGTTTGCCGATATTGTCGACGGAAAGACGAGGATGAAATCGGGGAAGAAAATGGTGAATCAACATAGAAAAATAAATGGTCATTTTCATAGTGTTTCTCACTATGCTTTCTTTGATGGAAACCCTAAATGGCCTGCTTCCAAATCCCATCTCACTTATGGTACATTTTATCTTCTATCCATATATAATTTTACGTGCATTTTTCATCTATATTACAACAATTTGGACTTTTTATGACAGAAAAATAAAGACAAATTAGGAGAGAGAAAAGTCTGCAATAAAACTTAAATTGACTTTTTTTCTGTGAAAAAAACAATTCGTTATCAAAATTTTCAAAATTTACTAAAAATTTTTATGTCACAAATTTACGATGGTATAAATAACTATCATTAAAGTTGGATTATAACAAGCTAGATTTTCTATTTTGATAGCACGTTAATTTGATTGTTGAATTTAAACTTATTCTAAATCTTTTATTCACATTCTTAACAGGATTTCTTCCGGGAACCCCATCGAAGGCAGTAGCTACGATAGGTCGAGCATTTAAGACATGGGGTGCAAACACTCACTTCAACTTCTCCCAAACATCTCGATACAAAAAAGCCGACATCAAAATAAGTTTTGAAACTGGCGACCATGGAGATGGCCATTCCTTCGATGGCGTTCGAGGAGTTATAGCTCATGGTTTTGCACCAAGAGATGGGAGATTACATTTCGACGCAGCCGAGTCATGGGCCGTCGGTGCTATTGCTGATTCGTTTGACTTGGAGACCATAGCTTTACATGAGATCGGACACCTTCTCGGCCTCTATCACAGCTCCATCGAAAGGGCCATTATGTGGCCAAGCATCATGGAAGGATCTACCAAGGGTTTGGATGCCGATGACATTGCAGGCATTAATGCATTATACAATGCTAATTGA

mRNA sequence

ATGGCATTCGAAACATTATTATCATTCTTAACGACCTCTTTTTTTCTTGTAATTGTCCTCTTCCCTTTGATATCATCTTCTCATCCAAAGAGTCATCTTTCCAATGGATTGGTGTTTCTAAAGAACCTCAATGGATGCAAGAAAGGTGACAAAGTAGAAGGAATTCACCAAGTGAAGGAATATCTTCAACATTTTGGCTATTTGAATAACTATGTTCAAAATTATTCTAAATCTTTTGATGATGATGAGTTCGATGGTGTTTTAGAGTCCGCCATTAAAACTTATCAACTCAACTACAATCTCAAGGCCACAGGTACTATCAATGCCAAGACGCTAGATCTTATGTCGAGACCTCGATGTGGGTTTGCCGATATTGTCGACGGAAAGACGAGGATGAAATCGGGGAAGAAAATGGTGAATCAACATAGAAAAATAAATGGTCATTTTCATAGTGTTTCTCACTATGCTTTCTTTGATGGAAACCCTAAATGGCCTGCTTCCAAATCCCATCTCACTTATGGATTTCTTCCGGGAACCCCATCGAAGGCAGTAGCTACGATAGGTCGAGCATTTAAGACATGGGGTGCAAACACTCACTTCAACTTCTCCCAAACATCTCGATACAAAAAAGCCGACATCAAAATAAGTTTTGAAACTGGCGACCATGGAGATGGCCATTCCTTCGATGGCGTTCGAGGAGTTATAGCTCATGGTTTTGCACCAAGAGATGGGAGATTACATTTCGACGCAGCCGAGTCATGGGCCGTCGGTGCTATTGCTGATTCGTTTGACTTGGAGACCATAGCTTTACATGAGATCGGACACCTTCTCGGCCTCTATCACAGCTCCATCGAAAGGGCCATTATGTGGCCAAGCATCATGGAAGGATCTACCAAGGGTTTGGATGCCGATGACATTGCAGGCATTAATGCATTATACAATGCTAATTGA

Coding sequence (CDS)

ATGGCATTCGAAACATTATTATCATTCTTAACGACCTCTTTTTTTCTTGTAATTGTCCTCTTCCCTTTGATATCATCTTCTCATCCAAAGAGTCATCTTTCCAATGGATTGGTGTTTCTAAAGAACCTCAATGGATGCAAGAAAGGTGACAAAGTAGAAGGAATTCACCAAGTGAAGGAATATCTTCAACATTTTGGCTATTTGAATAACTATGTTCAAAATTATTCTAAATCTTTTGATGATGATGAGTTCGATGGTGTTTTAGAGTCCGCCATTAAAACTTATCAACTCAACTACAATCTCAAGGCCACAGGTACTATCAATGCCAAGACGCTAGATCTTATGTCGAGACCTCGATGTGGGTTTGCCGATATTGTCGACGGAAAGACGAGGATGAAATCGGGGAAGAAAATGGTGAATCAACATAGAAAAATAAATGGTCATTTTCATAGTGTTTCTCACTATGCTTTCTTTGATGGAAACCCTAAATGGCCTGCTTCCAAATCCCATCTCACTTATGGATTTCTTCCGGGAACCCCATCGAAGGCAGTAGCTACGATAGGTCGAGCATTTAAGACATGGGGTGCAAACACTCACTTCAACTTCTCCCAAACATCTCGATACAAAAAAGCCGACATCAAAATAAGTTTTGAAACTGGCGACCATGGAGATGGCCATTCCTTCGATGGCGTTCGAGGAGTTATAGCTCATGGTTTTGCACCAAGAGATGGGAGATTACATTTCGACGCAGCCGAGTCATGGGCCGTCGGTGCTATTGCTGATTCGTTTGACTTGGAGACCATAGCTTTACATGAGATCGGACACCTTCTCGGCCTCTATCACAGCTCCATCGAAAGGGCCATTATGTGGCCAAGCATCATGGAAGGATCTACCAAGGGTTTGGATGCCGATGACATTGCAGGCATTAATGCATTATACAATGCTAATTGA

Homology

[Close]

BLAST of CSPI05G28500 vs. Swiss-Prot
Match: 2MMP_ARATH (Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana GN=2MMP PE=1 SV=1)

HSP 1 Score: 243.4 bits (620), Expect = 3.3e-63
Identity = 127/305 (41.64%), Postives = 178/305 (58.36%), Query Frame = 1


Query: 25  SSSHPKSHLSNGLVF---LKNLNGCKKGDKVEGIHQVKEYLQHFGYLNNYVQNYSKSFDD 84
           S++ P S  +   VF     N  GC  G  V+G++++K+Y Q FGY+    + +S +F D
Sbjct: 26  STAVPPSLRNTTRVFWDAFSNFTGCHHGQNVDGLYRIKKYFQRFGYI---PETFSGNFTD 85

Query: 85  DEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRCGFADIVDGKTRMKSGKKMVNQ 144
           D FD +L++A++ YQ N+NL  TG ++A T+  +  PRCG  D+V+G + M  G++   +
Sbjct: 86  D-FDDILKAAVELYQTNFNLNVTGELDALTIQHIVIPRCGNPDVVNGTSLMHGGRRKTFE 145

Query: 145 HRKINGHFHSVSHYAFFDGNPKWPASKSHLTYGFLPGTP--SKAVATIGRAFKTWGANTH 204
                 H H+V  Y  F G P+WP ++  LTY F P  P   +  +   RAF  W   T 
Sbjct: 146 VNFSRTHLHAVKRYTLFPGEPRWPRNRRDLTYAFDPKNPLTEEVKSVFSRAFGRWSDVTA 205

Query: 205 FNFSQTSRYKKADIKISFETGDHGDGHSFDGVRGVIAHGFAPRDGRLHFDAAESWAVGAI 264
            NF+ +  +  +DI I F TGDHGDG  FDGV G +AH F+P  G+ H DA E+W V   
Sbjct: 206 LNFTLSESFSTSDITIGFYTGDHGDGEPFDGVLGTLAHAFSPPSGKFHLDADENWVVSGD 265

Query: 265 ADSF-------DLETIALHEIGHLLGLYHSSIERAIMWPSIMEGSTK-GLDADDIAGINA 317
            DSF       DLE++A+HEIGHLLGL HSS+E +IM+P+I  G  K  L  DD+ GI  
Sbjct: 266 LDSFLSVTAAVDLESVAVHEIGHLLGLGHSSVEESIMYPTITTGKRKVDLTNDDVEGIQY 325

[Close]

BLAST of CSPI05G28500 vs. Swiss-Prot
Match: 3MMP_ARATH (Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana GN=3MMP PE=1 SV=1)

HSP 1 Score: 230.7 bits (587), Expect = 2.2e-59
Identity = 118/287 (41.11%), Postives = 168/287 (58.54%), Query Frame = 1


Query: 42  NLNGCKKGDKVEGIHQVKEYLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNL 101
           N  GC  G K +G++ +K+Y QHFGY+     N S +F DD FD +L++A++ YQ N+ L
Sbjct: 48  NFTGCHAGKKYDGLYMLKQYFQHFGYITE--TNLSGNFTDD-FDDILKNAVEMYQRNFQL 107

Query: 102 KATGTINAKTLDLMSRPRCGFADIVDGKTRMKSGKKMVNQHRKINGH-FHSVSHYAFFDG 161
             TG ++  TL  +  PRCG  D+V+G + M SG+K         G  FH+V HY+FF G
Sbjct: 108 NVTGVLDELTLKHVVIPRCGNPDVVNGTSTMHSGRKTFEVSFAGRGQRFHAVKHYSFFPG 167

Query: 162 NPKWPASKSHLTYGFLP--GTPSKAVATIGRAFKTWGANTHFNFSQTSRYKKADIKISFE 221
            P+WP ++  LTY F P      +  +   RAF  W   T   F++  R+  +DI I F 
Sbjct: 168 EPRWPRNRRDLTYAFDPRNALTEEVKSVFSRAFTRWEEVTPLTFTRVERFSTSDISIGFY 227

Query: 222 TGDHGDGHSFDGVRGVIAHGFAPRDGRLHFDAAESWAVG--------AIADSFDLETIAL 281
           +G+HGDG  FDG    +AH F+P  G  H D  E+W V         +++++ DLE++A+
Sbjct: 228 SGEHGDGEPFDGPMRTLAHAFSPPTGHFHLDGEENWIVSGEGGDGFISVSEAVDLESVAV 287

Query: 282 HEIGHLLGLYHSSIERAIMWPSIMEGSTK-GLDADDIAGINALYNAN 317
           HEIGHLLGL HSS+E +IM+P+I  G  K  L  DD+ G+  LY AN
Sbjct: 288 HEIGHLLGLGHSSVEGSIMYPTIRTGRRKVDLTTDDVEGVQYLYGAN 331

[Close]

BLAST of CSPI05G28500 vs. Swiss-Prot
Match: 5MMP_ARATH (Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana GN=5MMP PE=1 SV=1)

HSP 1 Score: 212.2 bits (539), Expect = 8.1e-54
Identity = 124/327 (37.92%), Postives = 170/327 (51.99%), Query Frame = 1


Query: 5   TLLSFLTTSFFLVIVLFPLISSSHPKSHLS---NGLVFLKNLNGCKKGDKVEGIHQVKEY 64
           T+L F  T   +    +  +SS  P   L+   N       L GC  G+ + G+ ++K+Y
Sbjct: 7   TILIFFFTVNPISAKFYTNVSSIPPLQFLNATQNAWETFSKLAGCHIGENINGLSKLKQY 66

Query: 65  LQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRCG 124
            + FGY+       +     D+FD VL+SAI TYQ N+NLK TG +++ TL  + +PRCG
Sbjct: 67  FRRFGYIT------TTGNCTDDFDDVLQSAINTYQKNFNLKVTGKLDSSTLRQIVKPRCG 126

Query: 125 FADIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLTYGFLP--GT 184
             D++DG + M  GK +            +   Y+FF G P+WP  K  LTY F P    
Sbjct: 127 NPDLIDGVSEMNGGKIL-----------RTTEKYSFFPGKPRWPKRKRDLTYAFAPQNNL 186

Query: 185 PSKAVATIGRAFKTWGANTHFNFSQTSRYKKADIKISFETGDHGDGHSFDGVRGVIAHGF 244
             +      RAF  W   T  NF+++    +ADI I F +G+HGDG  FDG  G +AH  
Sbjct: 187 TDEVKRVFSRAFTRWAEVTPLNFTRSESILRADIVIGFFSGEHGDGEPFDGAMGTLAHAS 246

Query: 245 APRDGRLHFDAAESWAVG---------AIADSFDLETIALHEIGHLLGLYHSSIERAIMW 304
           +P  G LH D  E W +           +    DLE++A+HEIGHLLGL HSS+E AIM+
Sbjct: 247 SPPTGMLHLDGDEDWLISNGEISRRILPVTTVVDLESVAVHEIGHLLGLGHSSVEDAIMF 306

Query: 305 PSIMEGSTK-GLDADDIAGINALYNAN 317
           P+I  G  K  L  DDI GI  LY  N
Sbjct: 307 PAISGGDRKVELAKDDIEGIQHLYGGN 316

[Close]

BLAST of CSPI05G28500 vs. Swiss-Prot
Match: 1MMP_ARATH (Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana GN=1MMP PE=1 SV=1)

HSP 1 Score: 205.7 bits (522), Expect = 7.6e-52
Identity = 121/281 (43.06%), Postives = 158/281 (56.23%), Query Frame = 1


Query: 49  GDKVEGIHQVKEYLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTIN 108
           G  V G+ ++K YL  FGY+N+  + +S     D FDG LESAI  YQ N  L  TG ++
Sbjct: 64  GSHVSGVSELKRYLHRFGYVNDGSEIFS-----DVFDGPLESAISLYQENLGLPITGRLD 123

Query: 109 AKTLDLMSRPRCGFADIVDGKTRMKSGKKMVNQHRKINGHF-HSVSHYAFFDGNPKWPAS 168
             T+ LMS PRCG +D                 H  IN  F H+ +HY +F+G PKW  +
Sbjct: 124 TSTVTLMSLPRCGVSDT----------------HMTINNDFLHTTAHYTYFNGKPKW--N 183

Query: 169 KSHLTYGF-----LPGTPSKAVATI-GRAFKTWGANTHFNFSQTSRYKKADIKISFETGD 228
           +  LTY       L    S+ V T+  RAF  W +    +F +   +  AD+KI F  GD
Sbjct: 184 RDTLTYAISKTHKLDYLTSEDVKTVFRRAFSQWSSVIPVSFEEVDDFTTADLKIGFYAGD 243

Query: 229 HGDGHSFDGVRGVIAHGFAPRDGRLHFDAAESWAV-----GAIADSFDLETIALHEIGHL 288
           HGDG  FDGV G +AH FAP +GRLH DAAE+W V     G+   + DLE++A HEIGHL
Sbjct: 244 HGDGLPFDGVLGTLAHAFAPENGRLHLDAAETWIVDDDLKGSSEVAVDLESVATHEIGHL 303

Query: 289 LGLYHSSIERAIMWPSIMEGSTK-GLDADDIAGINALYNAN 317
           LGL HSS E A+M+PS+   + K  L  DD+AG+  LY  N
Sbjct: 304 LGLGHSSQESAVMYPSLRPRTKKVDLTVDDVAGVLKLYGPN 321

[Close]

BLAST of CSPI05G28500 vs. Swiss-Prot
Match: MEP1_SOYBN (Metalloendoproteinase 1 OS=Glycine max PE=1 SV=2)

HSP 1 Score: 200.3 bits (508), Expect = 3.2e-50
Identity = 129/322 (40.06%), Postives = 165/322 (51.24%), Query Frame = 1


Query: 4   ETLLSFLTTSFFLVIVLFPLISSSHPKSHLSNGLVFLKNLNGCKKGDKVEGIHQVKEYLQ 63
           + LL  L T +FL   L P +S+  P  +  +G    K       G   +G+  VK Y  
Sbjct: 7   QELLVALATLYFLATSL-PSVSAHGP--YAWDGEATYK-FTTYHPGQNYKGLSNVKNYFH 66

Query: 64  HFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRCGFA 123
           H GY+ N     +  FDD+ FD  L SAIKTYQ NYNL  TG  +  TL  +  PRCG  
Sbjct: 67  HLGYIPN-----APHFDDN-FDDTLVSAIKTYQKNYNLNVTGKFDINTLKQIMTPRCGVP 126

Query: 124 DIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLTYGFLPGT--PS 183
           DI+    +  S              F  +S Y FF   P+W A  + LTY F P      
Sbjct: 127 DIIINTNKTTS--------------FGMISDYTFFKDMPRWQAGTTQLTYAFSPEPRLDD 186

Query: 184 KAVATIGRAFKTWGANTHFNFSQTSRYKKADIKISFETGDHGDGHSFDGVRGVIAHGFAP 243
              + I RAF  W    +  F +T+ Y+ A+IKI F + +HGD + FDG  G++ H FAP
Sbjct: 187 TFKSAIARAFSKWTPVVNIAFQETTSYETANIKILFASKNHGDPYPFDGPGGILGHAFAP 246

Query: 244 RDGRLHFDAAESWAVGA------IADSFDLETIALHEIGHLLGLYHSSIERAIMWPSIME 303
            DGR HFDA E W          +  +FDLE++A+HEIGHLLGL HSS  RAIM+PSI  
Sbjct: 247 TDGRCHFDADEYWVASGDVTKSPVTSAFDLESVAVHEIGHLLGLGHSSDLRAIMYPSIPP 304

Query: 304 GSTK-GLDADDIAGINALYNAN 317
            + K  L  DDI GI  LY  N
Sbjct: 307 RTRKVNLAQDDIDGIRKLYGIN 304

[Close]

BLAST of CSPI05G28500 vs. TrEMBL
Match: A0A0A0KU08_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G632130 PE=3 SV=1)

HSP 1 Score: 644.8 bits (1662), Expect = 5.4e-182
Identity = 316/316 (100.00%), Postives = 316/316 (100.00%), Query Frame = 1


Query: 1   MAFETLLSFLTTSFFLVIVLFPLISSSHPKSHLSNGLVFLKNLNGCKKGDKVEGIHQVKE 60
           MAFETLLSFLTTSFFLVIVLFPLISSSHPKSHLSNGLVFLKNLNGCKKGDKVEGIHQVKE
Sbjct: 1   MAFETLLSFLTTSFFLVIVLFPLISSSHPKSHLSNGLVFLKNLNGCKKGDKVEGIHQVKE 60

Query: 61  YLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRC 120
           YLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRC
Sbjct: 61  YLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRC 120

Query: 121 GFADIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLTYGFLPGTP 180
           GFADIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLTYGFLPGTP
Sbjct: 121 GFADIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLTYGFLPGTP 180

Query: 181 SKAVATIGRAFKTWGANTHFNFSQTSRYKKADIKISFETGDHGDGHSFDGVRGVIAHGFA 240
           SKAVATIGRAFKTWGANTHFNFSQTSRYKKADIKISFETGDHGDGHSFDGVRGVIAHGFA
Sbjct: 181 SKAVATIGRAFKTWGANTHFNFSQTSRYKKADIKISFETGDHGDGHSFDGVRGVIAHGFA 240

Query: 241 PRDGRLHFDAAESWAVGAIADSFDLETIALHEIGHLLGLYHSSIERAIMWPSIMEGSTKG 300
           PRDGRLHFDAAESWAVGAIADSFDLETIALHEIGHLLGLYHSSIERAIMWPSIMEGSTKG
Sbjct: 241 PRDGRLHFDAAESWAVGAIADSFDLETIALHEIGHLLGLYHSSIERAIMWPSIMEGSTKG 300

Query: 301 LDADDIAGINALYNAN 317
           LDADDIAGINALYNAN
Sbjct: 301 LDADDIAGINALYNAN 316

[Close]

BLAST of CSPI05G28500 vs. TrEMBL
Match: A0A0A0KS47_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G633160 PE=3 SV=1)

HSP 1 Score: 454.5 bits (1168), Expect = 1.0e-124
Identity = 221/316 (69.94%), Postives = 257/316 (81.33%), Query Frame = 1


Query: 1   MAFETLLSFLTTSFFLVIVLFPLISSSHPKSHLSNGLVFLKNLNGCKKGDKVEGIHQVKE 60
           M F+ L  F T + FL+I LFPLISSS PK    +GLVFLKNL GCKKGDKVEGIHQ+K+
Sbjct: 1   MGFQGLSLFFTFTLFLIISLFPLISSSQPKP--VDGLVFLKNLKGCKKGDKVEGIHQMKK 60

Query: 61  YLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRC 120
           YLQHFGYLNN V   S+S  DDEFD +LE A+KTYQ+NYNLK TGT++  TL  MS+PRC
Sbjct: 61  YLQHFGYLNNDVHIRSES-SDDEFDEILEFAVKTYQINYNLKVTGTLDNSTLMQMSKPRC 120

Query: 121 GFADIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLTYGFLPGTP 180
           G ADI++GKT MKSGK+ VNQHRKI+GHFH VSH+AFF+GN +WP SKSHLTYGFLPGTP
Sbjct: 121 GVADIINGKTSMKSGKRFVNQHRKISGHFHEVSHFAFFEGNLRWPDSKSHLTYGFLPGTP 180

Query: 181 SKAVATIGRAFKTWGANTHFNFSQTSRYKKADIKISFETGDHGDGHSFDGVRGVIAHGFA 240
           S+A++ + RAF TW ANTHF+FSQ S+Y+ ADIKISFE GDHGDG  FD V GV+AH FA
Sbjct: 181 SEAISPVNRAFTTWAANTHFSFSQESKYENADIKISFEKGDHGDGFPFDSVGGVLAHAFA 240

Query: 241 PRDGRLHFDAAESWAVGAIADSFDLETIALHEIGHLLGLYHSSIERAIMWPSIMEGSTKG 300
           P DGRLHFDA E WA GA+  S+D+ET+ALHEIGHLLGL+HSS+E AIMWPSIM G+TKG
Sbjct: 241 PTDGRLHFDAVEHWADGAVPKSYDMETVALHEIGHLLGLHHSSVEGAIMWPSIMGGATKG 300

Query: 301 LDADDIAGINALYNAN 317
           L ADDI GI  LY  +
Sbjct: 301 LHADDIEGIKVLYTTS 313

[Close]

BLAST of CSPI05G28500 vs. TrEMBL
Match: A0A0A0KS39_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G632120 PE=3 SV=1)

HSP 1 Score: 396.4 bits (1017), Expect = 3.4e-107
Identity = 202/319 (63.32%), Postives = 237/319 (74.29%), Query Frame = 1


Query: 1   MAFETLLSFLTTSFFLVIVLFPLISSS---HPKSH--LSNGLVFLKNLNGCKKGDKVEGI 60
           MA E L  F  T   L++  FPLISSS   H  SH   S+  VFL  L G +KGDKVEGI
Sbjct: 1   MALEELSFFTFT--LLLLTFFPLISSSQSNHSHSHGKASSQFVFLNQLYGSQKGDKVEGI 60

Query: 61  HQVKEYLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDLM 120
           HQ+K+YL  FGYLN   Q +S++ +DDEFD +LE+AIKTYQ NYNLK TGT++A T+  M
Sbjct: 61  HQLKKYLHQFGYLNEDAQIHSEN-NDDEFDELLETAIKTYQKNYNLKVTGTLDAMTIAQM 120

Query: 121 SRPRCGFADIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLTYGF 180
           S+PRCG ADI+ G T M+S KK       I GHFHSVSH+AFFDGNPKWPA+KSHLTYGF
Sbjct: 121 SKPRCGVADIIHGNTWMRSSKKRKQLEHGIVGHFHSVSHFAFFDGNPKWPATKSHLTYGF 180

Query: 181 LPGTPSKAVATIGRAFKTWGANTHFNFSQTSRYKKADIKISFETGDHGDGHSFDGVRGVI 240
           LPGTPS+ V+ + RAF TW AN+HF FSQ    + +DI I FETGDHGDG+ FDGV GV+
Sbjct: 181 LPGTPSETVSPVARAFATWAANSHFTFSQALDNQTSDITIGFETGDHGDGYPFDGVGGVV 240

Query: 241 AHGFAPRDGRLHFDAAESWAVGAIADSFDLETIALHEIGHLLGLYHSSIERAIMWPSIME 300
           AH F+P DGR H DA ESWA G ++DSFDLET+ALHEIGHLLGL HSS E AIMWP +  
Sbjct: 241 AHAFSPSDGRFHLDADESWANGVVSDSFDLETVALHEIGHLLGLQHSSTEGAIMWPQVPV 300

Query: 301 GSTKGLDADDIAGINALYN 315
           G++KGL  DDIAGI ALYN
Sbjct: 301 GASKGLHVDDIAGIKALYN 316

[Close]

BLAST of CSPI05G28500 vs. TrEMBL
Match: A0A067DG72_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g035681mg PE=3 SV=1)

HSP 1 Score: 355.1 bits (910), Expect = 8.6e-95
Identity = 187/320 (58.44%), Postives = 230/320 (71.88%), Query Frame = 1


Query: 8   SFLTTSFFLVIVLFPLISSSHPKSHL--------SNGLVFLKNLNGCKKGDKVEGIHQVK 67
           +FL+ S F VI +  L   SH  S L        S+   F+++L GC KGDK++GIH++K
Sbjct: 5   AFLSFSIFAVIQIILLSLLSHNYSVLAHSHAEKKSSPFKFIEHLKGCHKGDKIKGIHELK 64

Query: 68  EYLQHFGYLN--NYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSR 127
           EY++ FGYLN  NY +N S   DDD+FD +LESAIKTYQLNY+LK+TG ++A T+  M+ 
Sbjct: 65  EYMEKFGYLNYDNY-KNQSTHADDDDFDELLESAIKTYQLNYHLKSTGVLDANTVAKMTI 124

Query: 128 PRCGFADIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLTYGFLP 187
           PRCG ADIV+G TRM+SGKK   QH   N HF +VSHY+FF  +PKWPASK  LTY F P
Sbjct: 125 PRCGVADIVNGTTRMRSGKKR-QQH---NHHFRTVSHYSFFPRSPKWPASKFSLTYAFRP 184

Query: 188 GTPSKAVATIGRAFKTWGANTHFNFSQTSRYKKADIKISFETGDHGDGHSFDG---VRGV 247
           GT S A+  + RAF+TW ANT F FS+   +  ADIKISFE+GDHGDG+ FDG     G+
Sbjct: 185 GTRSDAITPVARAFQTWAANTQFKFSKIEDFANADIKISFESGDHGDGNPFDGRGGPEGI 244

Query: 248 IAHGFAPRDGRLHFDAAESWAVGAIADSFDLETIALHEIGHLLGLYHSSIERAIMWPSIM 307
           IAH FAP +GR H+DA E WAVGA+  +FDLET+ALHEIGHLLGL+HSS+E AIM+PSI 
Sbjct: 245 IAHAFAPTNGRFHYDADEPWAVGAVQGAFDLETVALHEIGHLLGLHHSSVEGAIMYPSIS 304

Query: 308 EGSTKGLDADDIAGINALYN 315
            G TKGL  DDI GI  LYN
Sbjct: 305 AGVTKGLHGDDIQGIRDLYN 319

[Close]

BLAST of CSPI05G28500 vs. TrEMBL
Match: B9RUG6_RICCO (Metalloendoproteinase 1, putative OS=Ricinus communis GN=RCOM_0852690 PE=3 SV=1)

HSP 1 Score: 354.4 bits (908), Expect = 1.5e-94
Identity = 172/303 (56.77%), Postives = 221/303 (72.94%), Query Frame = 1


Query: 17  VIVLFPLISS-----SHPKSHLSNGLVFLKNLNGCKKGDKVEGIHQVKEYLQHFGYLNNY 76
           +I+L  L+SS     SH K+  S+   FLK+L GC KGD ++GIH +K+YL++FGYL+  
Sbjct: 13  LILLISLLSSAALAHSHSKTEKSSAFDFLKHLQGCHKGDNLKGIHDLKKYLENFGYLSYK 72

Query: 77  VQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRCGFADIVDGKTR 136
            Q++S   +DD+FD +LE A+KTYQ NY+L  TG ++++T+  M  PRCG ADI++G TR
Sbjct: 73  NQSHS---NDDDFDDLLEYALKTYQFNYHLNVTGFLDSETVTKMMMPRCGVADIINGTTR 132

Query: 137 MKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLTYGFLPGTPSKAVATIGRAF 196
           M+S  K  N H   +  FH+VSHY FF GNP+WPASK HLTYGFLPGTP++A+  + +AF
Sbjct: 133 MQSSNK--NPHHHSSTSFHTVSHYEFFPGNPRWPASKYHLTYGFLPGTPNQAMEPVAKAF 192

Query: 197 KTWGANTHFNFSQTSRYKKADIKISFETGDHGDGHSFDGVRGVIAHGFAPRDGRLHFDAA 256
           +TW ANTHF F++   Y+ ADI I F  GDHGDG  FDG  G +AH FAP+DGR H+D  
Sbjct: 193 QTWAANTHFRFTRVQDYRAADITIGFHRGDHGDGSPFDGRGGTLAHAFAPQDGRFHYDGD 252

Query: 257 ESWAVGAIADSFDLETIALHEIGHLLGLYHSSIERAIMWPSIMEGSTKGLDADDIAGINA 315
           E WAVGA   +FD+ET+ALHEIGHLLGL HSS+E AIM PSI  G+TKGL +DDI GI A
Sbjct: 253 EHWAVGATQGAFDVETVALHEIGHLLGLGHSSVEGAIMHPSIQSGATKGLHSDDIQGIRA 310

[Close]

BLAST of CSPI05G28500 vs. TAIR10
Match: AT1G70170.1 (AT1G70170.1 matrix metalloproteinase)

HSP 1 Score: 243.4 bits (620), Expect = 1.8e-64
Identity = 127/305 (41.64%), Postives = 178/305 (58.36%), Query Frame = 1


Query: 25  SSSHPKSHLSNGLVF---LKNLNGCKKGDKVEGIHQVKEYLQHFGYLNNYVQNYSKSFDD 84
           S++ P S  +   VF     N  GC  G  V+G++++K+Y Q FGY+    + +S +F D
Sbjct: 26  STAVPPSLRNTTRVFWDAFSNFTGCHHGQNVDGLYRIKKYFQRFGYI---PETFSGNFTD 85

Query: 85  DEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRCGFADIVDGKTRMKSGKKMVNQ 144
           D FD +L++A++ YQ N+NL  TG ++A T+  +  PRCG  D+V+G + M  G++   +
Sbjct: 86  D-FDDILKAAVELYQTNFNLNVTGELDALTIQHIVIPRCGNPDVVNGTSLMHGGRRKTFE 145

Query: 145 HRKINGHFHSVSHYAFFDGNPKWPASKSHLTYGFLPGTP--SKAVATIGRAFKTWGANTH 204
                 H H+V  Y  F G P+WP ++  LTY F P  P   +  +   RAF  W   T 
Sbjct: 146 VNFSRTHLHAVKRYTLFPGEPRWPRNRRDLTYAFDPKNPLTEEVKSVFSRAFGRWSDVTA 205

Query: 205 FNFSQTSRYKKADIKISFETGDHGDGHSFDGVRGVIAHGFAPRDGRLHFDAAESWAVGAI 264
            NF+ +  +  +DI I F TGDHGDG  FDGV G +AH F+P  G+ H DA E+W V   
Sbjct: 206 LNFTLSESFSTSDITIGFYTGDHGDGEPFDGVLGTLAHAFSPPSGKFHLDADENWVVSGD 265

Query: 265 ADSF-------DLETIALHEIGHLLGLYHSSIERAIMWPSIMEGSTK-GLDADDIAGINA 317
            DSF       DLE++A+HEIGHLLGL HSS+E +IM+P+I  G  K  L  DD+ GI  
Sbjct: 266 LDSFLSVTAAVDLESVAVHEIGHLLGLGHSSVEESIMYPTITTGKRKVDLTNDDVEGIQY 325

[Close]

BLAST of CSPI05G28500 vs. TAIR10
Match: AT1G24140.1 (AT1G24140.1 Matrixin family protein)

HSP 1 Score: 230.7 bits (587), Expect = 1.2e-60
Identity = 118/287 (41.11%), Postives = 168/287 (58.54%), Query Frame = 1


Query: 42  NLNGCKKGDKVEGIHQVKEYLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNL 101
           N  GC  G K +G++ +K+Y QHFGY+     N S +F DD FD +L++A++ YQ N+ L
Sbjct: 48  NFTGCHAGKKYDGLYMLKQYFQHFGYITE--TNLSGNFTDD-FDDILKNAVEMYQRNFQL 107

Query: 102 KATGTINAKTLDLMSRPRCGFADIVDGKTRMKSGKKMVNQHRKINGH-FHSVSHYAFFDG 161
             TG ++  TL  +  PRCG  D+V+G + M SG+K         G  FH+V HY+FF G
Sbjct: 108 NVTGVLDELTLKHVVIPRCGNPDVVNGTSTMHSGRKTFEVSFAGRGQRFHAVKHYSFFPG 167

Query: 162 NPKWPASKSHLTYGFLP--GTPSKAVATIGRAFKTWGANTHFNFSQTSRYKKADIKISFE 221
            P+WP ++  LTY F P      +  +   RAF  W   T   F++  R+  +DI I F 
Sbjct: 168 EPRWPRNRRDLTYAFDPRNALTEEVKSVFSRAFTRWEEVTPLTFTRVERFSTSDISIGFY 227

Query: 222 TGDHGDGHSFDGVRGVIAHGFAPRDGRLHFDAAESWAVG--------AIADSFDLETIAL 281
           +G+HGDG  FDG    +AH F+P  G  H D  E+W V         +++++ DLE++A+
Sbjct: 228 SGEHGDGEPFDGPMRTLAHAFSPPTGHFHLDGEENWIVSGEGGDGFISVSEAVDLESVAV 287

Query: 282 HEIGHLLGLYHSSIERAIMWPSIMEGSTK-GLDADDIAGINALYNAN 317
           HEIGHLLGL HSS+E +IM+P+I  G  K  L  DD+ G+  LY AN
Sbjct: 288 HEIGHLLGLGHSSVEGSIMYPTIRTGRRKVDLTTDDVEGVQYLYGAN 331

[Close]

BLAST of CSPI05G28500 vs. TAIR10
Match: AT1G59970.1 (AT1G59970.1 Matrixin family protein)

HSP 1 Score: 212.2 bits (539), Expect = 4.6e-55
Identity = 124/327 (37.92%), Postives = 170/327 (51.99%), Query Frame = 1


Query: 5   TLLSFLTTSFFLVIVLFPLISSSHPKSHLS---NGLVFLKNLNGCKKGDKVEGIHQVKEY 64
           T+L F  T   +    +  +SS  P   L+   N       L GC  G+ + G+ ++K+Y
Sbjct: 7   TILIFFFTVNPISAKFYTNVSSIPPLQFLNATQNAWETFSKLAGCHIGENINGLSKLKQY 66

Query: 65  LQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRCG 124
            + FGY+       +     D+FD VL+SAI TYQ N+NLK TG +++ TL  + +PRCG
Sbjct: 67  FRRFGYIT------TTGNCTDDFDDVLQSAINTYQKNFNLKVTGKLDSSTLRQIVKPRCG 126

Query: 125 FADIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLTYGFLP--GT 184
             D++DG + M  GK +            +   Y+FF G P+WP  K  LTY F P    
Sbjct: 127 NPDLIDGVSEMNGGKIL-----------RTTEKYSFFPGKPRWPKRKRDLTYAFAPQNNL 186

Query: 185 PSKAVATIGRAFKTWGANTHFNFSQTSRYKKADIKISFETGDHGDGHSFDGVRGVIAHGF 244
             +      RAF  W   T  NF+++    +ADI I F +G+HGDG  FDG  G +AH  
Sbjct: 187 TDEVKRVFSRAFTRWAEVTPLNFTRSESILRADIVIGFFSGEHGDGEPFDGAMGTLAHAS 246

Query: 245 APRDGRLHFDAAESWAVG---------AIADSFDLETIALHEIGHLLGLYHSSIERAIMW 304
           +P  G LH D  E W +           +    DLE++A+HEIGHLLGL HSS+E AIM+
Sbjct: 247 SPPTGMLHLDGDEDWLISNGEISRRILPVTTVVDLESVAVHEIGHLLGLGHSSVEDAIMF 306

Query: 305 PSIMEGSTK-GLDADDIAGINALYNAN 317
           P+I  G  K  L  DDI GI  LY  N
Sbjct: 307 PAISGGDRKVELAKDDIEGIQHLYGGN 316

[Close]

BLAST of CSPI05G28500 vs. TAIR10
Match: AT4G16640.1 (AT4G16640.1 Matrixin family protein)

HSP 1 Score: 205.7 bits (522), Expect = 4.3e-53
Identity = 121/281 (43.06%), Postives = 158/281 (56.23%), Query Frame = 1


Query: 49  GDKVEGIHQVKEYLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTIN 108
           G  V G+ ++K YL  FGY+N+  + +S     D FDG LESAI  YQ N  L  TG ++
Sbjct: 64  GSHVSGVSELKRYLHRFGYVNDGSEIFS-----DVFDGPLESAISLYQENLGLPITGRLD 123

Query: 109 AKTLDLMSRPRCGFADIVDGKTRMKSGKKMVNQHRKINGHF-HSVSHYAFFDGNPKWPAS 168
             T+ LMS PRCG +D                 H  IN  F H+ +HY +F+G PKW  +
Sbjct: 124 TSTVTLMSLPRCGVSDT----------------HMTINNDFLHTTAHYTYFNGKPKW--N 183

Query: 169 KSHLTYGF-----LPGTPSKAVATI-GRAFKTWGANTHFNFSQTSRYKKADIKISFETGD 228
           +  LTY       L    S+ V T+  RAF  W +    +F +   +  AD+KI F  GD
Sbjct: 184 RDTLTYAISKTHKLDYLTSEDVKTVFRRAFSQWSSVIPVSFEEVDDFTTADLKIGFYAGD 243

Query: 229 HGDGHSFDGVRGVIAHGFAPRDGRLHFDAAESWAV-----GAIADSFDLETIALHEIGHL 288
           HGDG  FDGV G +AH FAP +GRLH DAAE+W V     G+   + DLE++A HEIGHL
Sbjct: 244 HGDGLPFDGVLGTLAHAFAPENGRLHLDAAETWIVDDDLKGSSEVAVDLESVATHEIGHL 303

Query: 289 LGLYHSSIERAIMWPSIMEGSTK-GLDADDIAGINALYNAN 317
           LGL HSS E A+M+PS+   + K  L  DD+AG+  LY  N
Sbjct: 304 LGLGHSSQESAVMYPSLRPRTKKVDLTVDDVAGVLKLYGPN 321

[Close]

BLAST of CSPI05G28500 vs. TAIR10
Match: AT2G45040.1 (AT2G45040.1 Matrixin family protein)

HSP 1 Score: 176.4 bits (446), Expect = 2.8e-44
Identity = 108/274 (39.42%), Postives = 144/274 (52.55%), Query Frame = 1


Query: 55  IHQVKEYLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDL 114
           I ++K +LQ +GYL       +K  DD  F    E A+  YQ N  L  TG  ++ TL  
Sbjct: 50  IPEIKRHLQQYGYLPQ-----NKESDDVSF----EQALVRYQKNLGLPITGKPDSDTLSQ 109

Query: 115 MSRPRCGFADIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKS-HLTY 174
           +  PRCGF D V+ KT                  FH+   Y +F G P+W       LTY
Sbjct: 110 ILLPRCGFPDDVEPKT----------------APFHTGKKYVYFPGRPRWTRDVPLKLTY 169

Query: 175 GFLPGT------PSKAVATIGRAFKTWGANTHFNFSQTSRYKKADIKISFETGDHGDGHS 234
            F          P+       RAF  W +    +F +T  Y  ADIKI F  GDHGDG  
Sbjct: 170 AFSQENLTPYLAPTDIRRVFRRAFGKWASVIPVSFIETEDYVIADIKIGFFNGDHGDGEP 229

Query: 235 FDGVRGVIAHGFAPRDGRLHFDAAESWAVGAIAD----SFDLETIALHEIGHLLGLYHSS 294
           FDGV GV+AH F+P +GRLH D AE+WAV    +    + DLE++A+HEIGH+LGL HSS
Sbjct: 230 FDGVLGVLAHTFSPENGRLHLDKAETWAVDFDEEKSSVAVDLESVAVHEIGHVLGLGHSS 289

Query: 295 IERAIMWPSIMEGSTK-GLDADDIAGINALYNAN 317
           ++ A M+P++   S K  L+ DD+ G+ +LY  N
Sbjct: 290 VKDAAMYPTLKPRSKKVNLNMDDVVGVQSLYGTN 298

[Close]

BLAST of CSPI05G28500 vs. NCBI nr
Match: gi|449449417|ref|XP_004142461.1| (PREDICTED: metalloendoproteinase 1-like [Cucumis sativus])

HSP 1 Score: 644.8 bits (1662), Expect = 7.8e-182
Identity = 316/316 (100.00%), Postives = 316/316 (100.00%), Query Frame = 1


Query: 1   MAFETLLSFLTTSFFLVIVLFPLISSSHPKSHLSNGLVFLKNLNGCKKGDKVEGIHQVKE 60
           MAFETLLSFLTTSFFLVIVLFPLISSSHPKSHLSNGLVFLKNLNGCKKGDKVEGIHQVKE
Sbjct: 1   MAFETLLSFLTTSFFLVIVLFPLISSSHPKSHLSNGLVFLKNLNGCKKGDKVEGIHQVKE 60

Query: 61  YLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRC 120
           YLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRC
Sbjct: 61  YLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRC 120

Query: 121 GFADIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLTYGFLPGTP 180
           GFADIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLTYGFLPGTP
Sbjct: 121 GFADIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLTYGFLPGTP 180

Query: 181 SKAVATIGRAFKTWGANTHFNFSQTSRYKKADIKISFETGDHGDGHSFDGVRGVIAHGFA 240
           SKAVATIGRAFKTWGANTHFNFSQTSRYKKADIKISFETGDHGDGHSFDGVRGVIAHGFA
Sbjct: 181 SKAVATIGRAFKTWGANTHFNFSQTSRYKKADIKISFETGDHGDGHSFDGVRGVIAHGFA 240

Query: 241 PRDGRLHFDAAESWAVGAIADSFDLETIALHEIGHLLGLYHSSIERAIMWPSIMEGSTKG 300
           PRDGRLHFDAAESWAVGAIADSFDLETIALHEIGHLLGLYHSSIERAIMWPSIMEGSTKG
Sbjct: 241 PRDGRLHFDAAESWAVGAIADSFDLETIALHEIGHLLGLYHSSIERAIMWPSIMEGSTKG 300

Query: 301 LDADDIAGINALYNAN 317
           LDADDIAGINALYNAN
Sbjct: 301 LDADDIAGINALYNAN 316

[Close]

BLAST of CSPI05G28500 vs. NCBI nr
Match: gi|659118019|ref|XP_008458907.1| (PREDICTED: metalloendoproteinase 1-like [Cucumis melo])

HSP 1 Score: 592.8 bits (1527), Expect = 3.5e-166
Identity = 283/316 (89.56%), Postives = 301/316 (95.25%), Query Frame = 1


Query: 1   MAFETLLSFLTTSFFLVIVLFPLISSSHPKSHLSNGLVFLKNLNGCKKGDKVEGIHQVKE 60
           M FETLLSFLTTSFFLVIVLFPLISSSHP  H SNGL FLKNLNGC+KGDKV GIHQ+KE
Sbjct: 1   MTFETLLSFLTTSFFLVIVLFPLISSSHPNIHHSNGLAFLKNLNGCRKGDKVAGIHQIKE 60

Query: 61  YLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRC 120
           YLQHFGYLNNY+QNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGT++AKTLDLMS+PRC
Sbjct: 61  YLQHFGYLNNYLQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTLDAKTLDLMSKPRC 120

Query: 121 GFADIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLTYGFLPGTP 180
           G ADI+DGKTRMKSGKKMVNQHRKINGHFH+VSHYAFFDGNPKWPASK HLTYGFLPGTP
Sbjct: 121 GVADIIDGKTRMKSGKKMVNQHRKINGHFHTVSHYAFFDGNPKWPASKFHLTYGFLPGTP 180

Query: 181 SKAVATIGRAFKTWGANTHFNFSQTSRYKKADIKISFETGDHGDGHSFDGVRGVIAHGFA 240
           SKAVA +GRAFKTWGA+THFNFS+ S+YKKADIKISF +GDHGDGHSFDGV GVIAHGFA
Sbjct: 181 SKAVAPVGRAFKTWGAHTHFNFSRASKYKKADIKISFRSGDHGDGHSFDGVGGVIAHGFA 240

Query: 241 PRDGRLHFDAAESWAVGAIADSFDLETIALHEIGHLLGLYHSSIERAIMWPSIMEGSTKG 300
           P DGRLHFDA ESWAVGA+ADSFDLET+ALHEIGHLLGL+HSS+E AIMWPSIMEG+TKG
Sbjct: 241 PTDGRLHFDAIESWAVGAVADSFDLETVALHEIGHLLGLHHSSVEGAIMWPSIMEGTTKG 300

Query: 301 LDADDIAGINALYNAN 317
           LDADDIAGINALYNAN
Sbjct: 301 LDADDIAGINALYNAN 316

[Close]

BLAST of CSPI05G28500 vs. NCBI nr
Match: gi|449449409|ref|XP_004142457.1| (PREDICTED: metalloendoproteinase 1-like [Cucumis sativus])

HSP 1 Score: 454.5 bits (1168), Expect = 1.5e-124
Identity = 221/316 (69.94%), Postives = 257/316 (81.33%), Query Frame = 1


Query: 1   MAFETLLSFLTTSFFLVIVLFPLISSSHPKSHLSNGLVFLKNLNGCKKGDKVEGIHQVKE 60
           M F+ L  F T + FL+I LFPLISSS PK    +GLVFLKNL GCKKGDKVEGIHQ+K+
Sbjct: 1   MGFQGLSLFFTFTLFLIISLFPLISSSQPKP--VDGLVFLKNLKGCKKGDKVEGIHQMKK 60

Query: 61  YLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDLMSRPRC 120
           YLQHFGYLNN V   S+S  DDEFD +LE A+KTYQ+NYNLK TGT++  TL  MS+PRC
Sbjct: 61  YLQHFGYLNNDVHIRSES-SDDEFDEILEFAVKTYQINYNLKVTGTLDNSTLMQMSKPRC 120

Query: 121 GFADIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLTYGFLPGTP 180
           G ADI++GKT MKSGK+ VNQHRKI+GHFH VSH+AFF+GN +WP SKSHLTYGFLPGTP
Sbjct: 121 GVADIINGKTSMKSGKRFVNQHRKISGHFHEVSHFAFFEGNLRWPDSKSHLTYGFLPGTP 180

Query: 181 SKAVATIGRAFKTWGANTHFNFSQTSRYKKADIKISFETGDHGDGHSFDGVRGVIAHGFA 240
           S+A++ + RAF TW ANTHF+FSQ S+Y+ ADIKISFE GDHGDG  FD V GV+AH FA
Sbjct: 181 SEAISPVNRAFTTWAANTHFSFSQESKYENADIKISFEKGDHGDGFPFDSVGGVLAHAFA 240

Query: 241 PRDGRLHFDAAESWAVGAIADSFDLETIALHEIGHLLGLYHSSIERAIMWPSIMEGSTKG 300
           P DGRLHFDA E WA GA+  S+D+ET+ALHEIGHLLGL+HSS+E AIMWPSIM G+TKG
Sbjct: 241 PTDGRLHFDAVEHWADGAVPKSYDMETVALHEIGHLLGLHHSSVEGAIMWPSIMGGATKG 300

Query: 301 LDADDIAGINALYNAN 317
           L ADDI GI  LY  +
Sbjct: 301 LHADDIEGIKVLYTTS 313

[Close]

BLAST of CSPI05G28500 vs. NCBI nr
Match: gi|659117646|ref|XP_008458713.1| (PREDICTED: metalloendoproteinase 1-like [Cucumis melo])

HSP 1 Score: 434.9 bits (1117), Expect = 1.2e-118
Identity = 201/282 (71.28%), Postives = 239/282 (84.75%), Query Frame = 1


Query: 35  NGLVFLKNLNGCKKGDKVEGIHQVKEYLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKT 94
           +GLVFLKNLNGCKKGDKVEGIHQ+K+YLQHFGY+NN V  +SKS  DDEFD +LE A+KT
Sbjct: 2   DGLVFLKNLNGCKKGDKVEGIHQMKKYLQHFGYMNNDVHIHSKS-SDDEFDEILEFAVKT 61

Query: 95  YQLNYNLKATGTINAKTLDLMSRPRCGFADIVDGKTRMKSGKKMVNQHRKINGHFHSVSH 154
           YQ+NYNLKATGT++  T+  MS+PRCG ADI++G+T MKSGK++VN+HRKI GHFH VSH
Sbjct: 62  YQINYNLKATGTLDNSTISQMSKPRCGVADIINGRTGMKSGKRLVNRHRKIIGHFHEVSH 121

Query: 155 YAFFDGNPKWPASKSHLTYGFLPGTPSKAVATIGRAFKTWGANTHFNFSQTSRYKKADIK 214
           +AFF+GN KWP SKSHLTYGFLPGTPS+A++ + RAF TW ANTHF+FSQ S Y+ ADIK
Sbjct: 122 FAFFEGNLKWPNSKSHLTYGFLPGTPSEAISPVNRAFTTWAANTHFSFSQESNYENADIK 181

Query: 215 ISFETGDHGDGHSFDGVRGVIAHGFAPRDGRLHFDAAESWAVGAIADSFDLETIALHEIG 274
           ++FE GDHGDG  FD V GV+AH FAP DGRLHFDA E WA GA+ +S+D+ET+ALHEIG
Sbjct: 182 VTFERGDHGDGFPFDSVGGVLAHAFAPTDGRLHFDAVEHWADGAVPNSYDMETVALHEIG 241

Query: 275 HLLGLYHSSIERAIMWPSIMEGSTKGLDADDIAGINALYNAN 317
           HLLGL+HSS+E AIMWPSIM G+TKGL ADDI GI  LY  +
Sbjct: 242 HLLGLHHSSVEGAIMWPSIMGGTTKGLHADDIEGIKVLYTTS 282

[Close]

BLAST of CSPI05G28500 vs. NCBI nr
Match: gi|449449419|ref|XP_004142462.1| (PREDICTED: metalloendoproteinase 1 [Cucumis sativus])

HSP 1 Score: 396.4 bits (1017), Expect = 4.8e-107
Identity = 202/319 (63.32%), Postives = 237/319 (74.29%), Query Frame = 1


Query: 1   MAFETLLSFLTTSFFLVIVLFPLISSS---HPKSH--LSNGLVFLKNLNGCKKGDKVEGI 60
           MA E L  F  T   L++  FPLISSS   H  SH   S+  VFL  L G +KGDKVEGI
Sbjct: 1   MALEELSFFTFT--LLLLTFFPLISSSQSNHSHSHGKASSQFVFLNQLYGSQKGDKVEGI 60

Query: 61  HQVKEYLQHFGYLNNYVQNYSKSFDDDEFDGVLESAIKTYQLNYNLKATGTINAKTLDLM 120
           HQ+K+YL  FGYLN   Q +S++ +DDEFD +LE+AIKTYQ NYNLK TGT++A T+  M
Sbjct: 61  HQLKKYLHQFGYLNEDAQIHSEN-NDDEFDELLETAIKTYQKNYNLKVTGTLDAMTIAQM 120

Query: 121 SRPRCGFADIVDGKTRMKSGKKMVNQHRKINGHFHSVSHYAFFDGNPKWPASKSHLTYGF 180
           S+PRCG ADI+ G T M+S KK       I GHFHSVSH+AFFDGNPKWPA+KSHLTYGF
Sbjct: 121 SKPRCGVADIIHGNTWMRSSKKRKQLEHGIVGHFHSVSHFAFFDGNPKWPATKSHLTYGF 180

Query: 181 LPGTPSKAVATIGRAFKTWGANTHFNFSQTSRYKKADIKISFETGDHGDGHSFDGVRGVI 240
           LPGTPS+ V+ + RAF TW AN+HF FSQ    + +DI I FETGDHGDG+ FDGV GV+
Sbjct: 181 LPGTPSETVSPVARAFATWAANSHFTFSQALDNQTSDITIGFETGDHGDGYPFDGVGGVV 240

Query: 241 AHGFAPRDGRLHFDAAESWAVGAIADSFDLETIALHEIGHLLGLYHSSIERAIMWPSIME 300
           AH F+P DGR H DA ESWA G ++DSFDLET+ALHEIGHLLGL HSS E AIMWP +  
Sbjct: 241 AHAFSPSDGRFHLDADESWANGVVSDSFDLETVALHEIGHLLGLQHSSTEGAIMWPQVPV 300

Query: 301 GSTKGLDADDIAGINALYN 315
           G++KGL  DDIAGI ALYN
Sbjct: 301 GASKGLHVDDIAGIKALYN 316

The following BLAST results are available for this feature:

BLAST of CSPI05G28500 vs. Swiss-Prot


Match Name	E-value	Identity	Description
2MMP_ARATH	3.3e-63	41.64	Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana GN=2MMP PE=1 SV=1	[more]
3MMP_ARATH	2.2e-59	41.11	Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana GN=3MMP PE=1 SV=1	[more]
5MMP_ARATH	8.1e-54	37.92	Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana GN=5MMP PE=1 SV=1	[more]
1MMP_ARATH	7.6e-52	43.06	Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana GN=1MMP PE=1 SV=1	[more]
MEP1_SOYBN	3.2e-50	40.06	Metalloendoproteinase 1 OS=Glycine max PE=1 SV=2	[more]

BLAST of CSPI05G28500 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KU08_CUCSA	5.4e-182	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G632130 PE=3 SV=1	[more]
A0A0A0KS47_CUCSA	1.0e-124	69.94	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G633160 PE=3 SV=1	[more]
A0A0A0KS39_CUCSA	3.4e-107	63.32	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G632120 PE=3 SV=1	[more]
A0A067DG72_CITSI	8.6e-95	58.44	Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g035681mg PE=3 SV=1	[more]
B9RUG6_RICCO	1.5e-94	56.77	Metalloendoproteinase 1, putative OS=Ricinus communis GN=RCOM_0852690 PE=3 SV=1	[more]

BLAST of CSPI05G28500 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G70170.1	1.8e-64	41.64	matrix metalloproteinase	[more]
AT1G24140.1	1.2e-60	41.11	Matrixin family protein	[more]
AT1G59970.1	4.6e-55	37.92	Matrixin family protein	[more]
AT4G16640.1	4.3e-53	43.06	Matrixin family protein	[more]
AT2G45040.1	2.8e-44	39.42	Matrixin family protein	[more]

BLAST of CSPI05G28500 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449449417\|ref\|XP_004142461.1\|	7.8e-182	100.00	PREDICTED: metalloendoproteinase 1-like [Cucumis sativus]	[more]
gi\|659118019\|ref\|XP_008458907.1\|	3.5e-166	89.56	PREDICTED: metalloendoproteinase 1-like [Cucumis melo]	[more]
gi\|449449409\|ref\|XP_004142457.1\|	1.5e-124	69.94	PREDICTED: metalloendoproteinase 1-like [Cucumis sativus]	[more]
gi\|659117646\|ref\|XP_008458713.1\|	1.2e-118	71.28	PREDICTED: metalloendoproteinase 1-like [Cucumis melo]	[more]
gi\|449449419\|ref\|XP_004142462.1\|	4.8e-107	63.32	PREDICTED: metalloendoproteinase 1 [Cucumis sativus]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR001818	Pept_M10_metallopeptidase
IPR002477	Peptidoglycan-bd-like
IPR006026	Peptidase_Metallo
IPR021190	Pept_M10A
IPR024079	MetalloPept_cat_dom_sf

Vocabulary: **Molecular Function**
Term	Definition
GO:0004222	metalloendopeptidase activity
GO:0008270	zinc ion binding
GO:0008237	metallopeptidase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0006508	proteolysis

Vocabulary: **Cellular Component**
Term	Definition
GO:0031012	extracellular matrix

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
cellular_component	GO:0031012	extracellular matrix
molecular_function	GO:0004222	metalloendopeptidase activity
molecular_function	GO:0008270	zinc ion binding
molecular_function	GO:0008237	metallopeptidase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI05G28500.1	CSPI05G28500.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001818	Peptidase M10, metallopeptidase	PFAM	PF00413	Peptidase_M10	coord: 164..313 score: 5.7
IPR002477	Peptidoglycan binding-like	GENE3D	G3DSA:1.10.101.10		coord: 54..116 score: 5.6
IPR002477	Peptidoglycan binding-like	PFAM	PF01471	PG_binding_1	coord: 54..115 score: 2.5
IPR002477	Peptidoglycan binding-like	unknown	SSF47090	PGBD-like	coord: 46..125 score: 5.81
IPR006026	Peptidase, metallopeptidase	SMART	SM00235	col_5	coord: 160..315 score: 8.7
IPR021190	Peptidase M10A	PRINTS	PR00138	MATRIXIN	coord: 187..202 score: 3.0E-32coord: 268..293 score: 3.0E-32coord: 301..314 score: 3.0E-32coord: 211..239 score: 3.0E-32coord: 115..128 score: 3.0
IPR024079	Metallopeptidase, catalytic domain	GENE3D	G3DSA:3.40.390.10		coord: 117..316 score: 6.2
None	No IPR available	PANTHER	PTHR10201	MATRIX METALLOPROTEINASE	coord: 4..128 score: 6.7E-113coord: 150..316 score: 6.7E
None	No IPR available	PANTHER	PTHR10201:SF140	F20P5.11 PROTEIN	coord: 4..128 score: 6.7E-113coord: 150..316 score: 6.7E
None	No IPR available	unknown	SSF55486	Metalloproteases ("zincins"), catalytic domain	coord: 156..316 score: 5.63

Synteny

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CSPI05G28500	Cucumber (Chinese Long) v3	cpicucB259
CSPI05G28500	Cucumber (Chinese Long) v3	cpicucB284
CSPI05G28500	Watermelon (97103) v2	cpiwmbB357
CSPI05G28500	Watermelon (97103) v2	cpiwmbB429
CSPI05G28500	Wax gourd	cpiwgoB473
CSPI05G28500	Wax gourd	cpiwgoB505
CSPI05G28500	Wild cucumber (PI 183967)	cpicpiB036
CSPI05G28500	Cucumber (Gy14) v1	cgycpiB110
CSPI05G28500	Cucumber (Gy14) v1	cgycpiB236
CSPI05G28500	Cucurbita maxima (Rimu)	cmacpiB304
CSPI05G28500	Cucurbita maxima (Rimu)	cmacpiB730
CSPI05G28500	Cucurbita moschata (Rifu)	cmocpiB216
CSPI05G28500	Cucurbita moschata (Rifu)	cmocpiB296
CSPI05G28500	Cucurbita moschata (Rifu)	cmocpiB722
CSPI05G28500	Cucumber (Chinese Long) v2	cpicuB219
CSPI05G28500	Cucumber (Chinese Long) v2	cpicuB239
CSPI05G28500	Melon (DHL92) v3.5.1	cpimeB349
CSPI05G28500	Melon (DHL92) v3.5.1	cpimeB382
CSPI05G28500	Watermelon (Charleston Gray)	cpiwcgB384
CSPI05G28500	Watermelon (Charleston Gray)	cpiwcgB356
CSPI05G28500	Watermelon (Charleston Gray)	cpiwcgB360
CSPI05G28500	Watermelon (97103) v1	cpiwmB370
CSPI05G28500	Watermelon (97103) v1	cpiwmB432
CSPI05G28500	Cucurbita pepo (Zucchini)	cpecpiB177
CSPI05G28500	Cucurbita pepo (Zucchini)	cpecpiB414
CSPI05G28500	Bottle gourd (USVL1VR-Ls)	cpilsiB355
CSPI05G28500	Bottle gourd (USVL1VR-Ls)	cpilsiB352
CSPI05G28500	Melon (DHL92) v3.6.1	cpimedB339
CSPI05G28500	Melon (DHL92) v3.6.1	cpimedB377
CSPI05G28500	Cucumber (Gy14) v2	cgybcpiB035
CSPI05G28500	Cucumber (Gy14) v2	cgybcpiB225
CSPI05G28500	Silver-seed gourd	carcpiB0297
CSPI05G28500	Silver-seed gourd	carcpiB0722