BLAST of CSPI05G27660 vs. Swiss-Prot
Match:
AED3_ARATH (Aspartyl protease AED3 OS=Arabidopsis thaliana GN=AED3 PE=1 SV=1)
HSP 1 Score: 406.0 bits (1042), Expect = 5.3e-112
Identity = 227/456 (49.78%), Postives = 304/456 (66.67%), Query Frame = 1
Query: 1 MNNTKSPFLLLLLLLLLHLFSISTAKSHIPSNCNPAA-DRSSTLQVFHIFSPCSPFRPSK 60
M ++ F L LLL F+ +T + C AA D S L + I + CSPF P+
Sbjct: 1 MASSSLHFFFFLTLLLPFTFTTATRDT-----CATAAPDGSDDLSIIPINAKCSPFAPTH 60
Query: 61 -PLSWADNVLQMQAKDQARLQFLSSLVARR---SFVPIASARQLIQSPTFVVRAKIGTPA 120
S D VL M + D RL +LSSLVA + + VP+AS QL +VVRAK+GTP
Sbjct: 61 VSASVIDTVLHMASSDSHRLTYLSSLVAGKPKPTSVPVASGNQL-HIGNYVVRAKLGTPP 120
Query: 121 QTLLLALDTSNDAAWIPCSGCIGCPS-TTVFSSDKSSSFRPLPCQSSQCNQV-----PNP 180
Q + + LDTSNDA W+PCSGC GC + +T F+++ SS++ + C ++QC Q P+
Sbjct: 121 QLMFMVLDTSNDAVWLPCSGCSGCSNASTSFNTNSSSTYSTVSCSTAQCTQARGLTCPSS 180
Query: 181 SCSGSACGFNLTYGS-STVAADLVQDNLTLATDSVPSYTFGCIRKATGSSVPPQGLLGLG 240
S S C FN +YG S+ +A LVQD LTLA D +P+++FGCI A+G+S+PPQGL+GLG
Sbjct: 181 SPQPSVCSFNQSYGGDSSFSASLVQDTLTLAPDVIPNFSFGCINSASGNSLPPQGLMGLG 240
Query: 241 RGPLSLLGQSQSLYQSTFSYCLPSFKSVNFSGSLRLGPVAQPIRIKYTPLLRNPRRSSLY 300
RGP+SL+ Q+ SLY FSYCLPSF+S FSGSL+LG + QP I+YTPLLRNPRR SLY
Sbjct: 241 RGPMSLVSQTTSLYSGVFSYCLPSFRSFYFSGSLKLGLLGQPKSIRYTPLLRNPRRPSLY 300
Query: 301 YVNLISIRVGRKIVDIPPSALAFNSATGAGTVIDSGTTFTRLVAPAYTAVRDEFRRRVGR 360
YVNL + VG V + P L F++ +GAGT+IDSGT TR P Y A+RDEFR++V
Sbjct: 301 YVNLTGVSVGSVQVPVDPVYLTFDANSGAGTIIDSGTVITRFAQPVYEAIRDEFRKQVNV 360
Query: 361 NVTVSSLGGFDTCYTV--PIISPTITFMFAGMNVTLPPDNFLIHSTAGSTTCLAMAAAPD 420
+ + S+LG FDTC++ ++P IT +++ LP +N LIHS+AG+ TCL+MA
Sbjct: 361 S-SFSTLGAFDTCFSADNENVAPKITLHMTSLDLKLPMENTLIHSSAGTLTCLSMAGIRQ 420
Query: 421 NVNSVLNVIASMQQQNHRILFDIPNSRVGVARESCS 443
N N+VLNVIA++QQQN RILFD+PNSR+G+A E C+
Sbjct: 421 NANAVLNVIANLQQQNLRILFDVPNSRIGIAPEPCN 449
BLAST of CSPI05G27660 vs. Swiss-Prot
Match:
AP25_ORYSJ (Aspartyl protease 25 OS=Oryza sativa subsp. japonica GN=AP25 PE=2 SV=1)
HSP 1 Score: 363.2 bits (931), Expect = 4.0e-99
Identity = 207/430 (48.14%), Postives = 274/430 (63.72%), Query Frame = 1
Query: 37 ADRSSTLQVFHIFSPCSPFRPSKPLSWADNVLQMQAKDQARLQFLSSLVARR--SFVPIA 96
A ++ L V+H P SP PL ++++ + D ARL FLSS A S P+A
Sbjct: 19 AAAAAELSVYHNVHPSSP----SPL---ESIIALARDDDARLLFLSSKAATAGVSSAPVA 78
Query: 97 SARQLIQSPTFVVRAKIGTPAQTLLLALDTSNDAAWIPCSGCIGCPSTTVFSSDKSSSFR 156
S + P++VVRA +G+P+Q LLLALDTS DA W CS C CPS+++F+ SSS+
Sbjct: 79 SGQA---PPSYVVRAGLGSPSQQLLLALDTSADATWAHCSPCGTCPSSSLFAPANSSSYA 138
Query: 157 PLPCQSSQC-----NQVPNPSCSGSA---------CGFNLTYGSSTVAADLVQDNLTLAT 216
LPC SS C P P G A C F+ + ++ A L D L L
Sbjct: 139 SLPCSSSWCPLFQGQACPAPQGGGDAAPPPATLPTCAFSKPFADASFQAALASDTLRLGK 198
Query: 217 DSVPSYTFGCIRKATG--SSVPPQGLLGLGRGPLSLLGQSQSLYQSTFSYCLPSFKSVNF 276
D++P+YTFGC+ TG +++P QGLLGLGRGP++LL Q+ SLY FSYCLPS++S F
Sbjct: 199 DAIPNYTFGCVSSVTGPTTNMPRQGLLGLGRGPMALLSQAGSLYNGVFSYCLPSYRSYYF 258
Query: 277 SGSLRLGPVA-QPIRIKYTPLLRNPRRSSLYYVNLISIRVGRKIVDIPPSALAFNSATGA 336
SGSLRLG QP ++YTP+LRNP RSSLYYVN+ + VG V +P + AF++ATGA
Sbjct: 259 SGSLRLGAGGGQPRSVRYTPMLRNPHRSSLYYVNVTGLSVGHAWVKVPAGSFAFDAATGA 318
Query: 337 GTVIDSGTTFTRLVAPAYTAVRDEFRRRVGRNVTVSSLGGFDTCYTVPII----SPTIT- 396
GTV+DSGT TR AP Y A+R+EFRR+V +SLG FDTC+ + +P +T
Sbjct: 319 GTVVDSGTVITRWTAPVYAALREEFRRQVAAPSGYTSLGAFDTCFNTDEVAAGGAPAVTV 378
Query: 397 FMFAGMNVTLPPDNFLIHSTAGSTTCLAMAAAPDNVNSVLNVIASMQQQNHRILFDIPNS 443
M G+++ LP +N LIHS+A CLAMA AP NVNSV+NVIA++QQQN R++FD+ NS
Sbjct: 379 HMDGGVDLALPMENTLIHSSATPLACLAMAEAPQNVNSVVNVIANLQQQNIRVVFDVANS 438
BLAST of CSPI05G27660 vs. Swiss-Prot
Match:
APF2_ARATH (Aspartyl protease family protein 2 OS=Arabidopsis thaliana GN=APF2 PE=2 SV=1)
HSP 1 Score: 187.2 bits (474), Expect = 3.9e-46
Identity = 132/441 (29.93%), Postives = 208/441 (47.17%), Query Frame = 1
Query: 21 SISTAKSHIPSNCNPAADRSSTLQVFHIFSPCSPFRPSKPLSWADNVLQMQAKDQARLQF 80
S S +S S + + S TL + HI + S P + S + LQ ++ +
Sbjct: 52 SESLLESEFESGSDSESSSSITLNLDHIDALSSNKTPDELFS---SRLQRDSRRVKSIAT 111
Query: 81 LSSLVARRSFV----PIASARQLIQ-----SPTFVVRAKIGTPAQTLLLALDTSNDAAWI 140
L++ + R+ P + ++ S + R +GTPA+ + + LDT +D W+
Sbjct: 112 LAAQIPGRNVTHAPRPGGFSSSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWL 171
Query: 141 PCSGCIGCPSTT--VFSSDKSSSFRPLPCQSSQCNQVPNPSCSG--SACGFNLTYGS-ST 200
C+ C C S + +F KS ++ +PC S C ++ + C+ C + ++YG S
Sbjct: 172 QCAPCRRCYSQSDPIFDPRKSKTYATIPCSSPHCRRLDSAGCNTRRKTCLYQVSYGDGSF 231
Query: 201 VAADLVQDNLTLATDSVPSYTFGCIRKATGSSVPPQGLLGLGRGPLSLLGQSQSLYQSTF 260
D + LT + V GC G V GLLGLG+G LS GQ+ + F
Sbjct: 232 TVGDFSTETLTFRRNRVKGVALGCGHDNEGLFVGAAGLLGLGKGKLSFPGQTGHRFNQKF 291
Query: 261 SYCLPSFKSVNFSGSLRLGPVAQPIRIKYTPLLRNPRRSSLYYVNLISIRVG-RKIVDIP 320
SYCL + + S+ G A ++TPLL NP+ + YYV L+ I VG ++ +
Sbjct: 292 SYCLVDRSASSKPSSVVFGNAAVSRIARFTPLLSNPKLDTFYYVGLLGISVGGTRVPGVT 351
Query: 321 PSALAFNSATGAGTVIDSGTTFTRLVAPAYTAVRDEFRRRVGRNVTVSSLGGFDTCYTVP 380
S + G +IDSGT+ TRL+ PAY A+RD FR FDTC+ +
Sbjct: 352 ASLFKLDQIGNGGVIIDSGTSVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLFDTCFDLS 411
Query: 381 IIS----PTITFMFAGMNVTLPPDNFLIHSTAGSTTCLAMAAAPDNVNSVLNVIASMQQQ 440
++ PT+ F G +V+LP N+LI C A A L++I ++QQQ
Sbjct: 412 NMNEVKVPTVVLHFRGADVSLPATNYLIPVDTNGKFCFAFAGTMGG----LSIIGNIQQQ 471
Query: 441 NHRILFDIPNSRVGVARESCS 443
R+++D+ +SRVG A C+
Sbjct: 472 GFRVVYDLASSRVGFAPGGCA 485
BLAST of CSPI05G27660 vs. Swiss-Prot
Match:
ASPG2_ARATH (Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana GN=ASPG2 PE=2 SV=1)
HSP 1 Score: 185.7 bits (470), Expect = 1.1e-45
Identity = 117/348 (33.62%), Postives = 167/348 (47.99%), Query Frame = 1
Query: 102 SPTFVVRAKIGTPAQTLLLALDTSNDAAWIPCSGCIGC--PSTTVFSSDKSSSFRPLPCQ 161
S + VR +G+P + + +D+ +D W+ C C C S VF KS S+ + C
Sbjct: 128 SGEYFVRIGVGSPPRDQYMVIDSGSDMVWVQCQPCKLCYKQSDPVFDPAKSGSYTGVSCG 187
Query: 162 SSQCNQVPNPSCSGSACGFNLTYGS-STVAADLVQDNLTLATDSVPSYTFGCIRKATGSS 221
SS C+++ N C C + + YG S L + LT A V + GC + G
Sbjct: 188 SSVCDRIENSGCHSGGCRYEVMYGDGSYTKGTLALETLTFAKTVVRNVAMGCGHRNRGMF 247
Query: 222 VPPQGLLGLGRGPLSLLGQSQSLYQSTFSYCLPSFKSVNFSGSLRLGPVAQPIRIKYTPL 281
+ GLLG+G G +S +GQ F YCL S + + +GSL G A P+ + PL
Sbjct: 248 IGAAGLLGIGGGSMSFVGQLSGQTGGAFGYCLVS-RGTDSTGSLVFGREALPVGASWVPL 307
Query: 282 LRNPRRSSLYYVNLISIRVGRKIVDIPPSALAFNSATGAGTVIDSGTTFTRLVAPAYTAV 341
+RNPR S YYV L + VG + +P G V+D+GT TRL AY A
Sbjct: 308 VRNPRAPSFYYVGLKGLGVGGVRIPLPDGVFDLTETGDGGVVMDTGTAVTRLPTAAYVAF 367
Query: 342 RDEFRRRVGRNVTVSSLGGFDTCYT----VPIISPTITFMFA-GMNVTLPPDNFLIHSTA 401
RD F+ + S + FDTCY V + PT++F F G +TLP NFL+
Sbjct: 368 RDGFKSQTANLPRASGVSIFDTCYDLSGFVSVRVPTVSFYFTEGPVLTLPARNFLMPVDD 427
Query: 402 GSTTCLAMAAAPDNVNSVLNVIASMQQQNHRILFDIPNSRVGVARESC 442
T C A AA+P L++I ++QQ+ ++ FD N VG C
Sbjct: 428 SGTYCFAFAASPTG----LSIIGNIQQEGIQVSFDGANGFVGFGPNVC 470
BLAST of CSPI05G27660 vs. Swiss-Prot
Match:
NEP1_NEPGR (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis GN=nep1 PE=1 SV=1)
HSP 1 Score: 182.2 bits (461), Expect = 1.3e-44
Identity = 123/351 (35.04%), Postives = 177/351 (50.43%), Query Frame = 1
Query: 105 FVVRAKIGTPAQTLLLALDTSNDAAWIPCSGCIGC--PSTTVFSSDKSSSFRPLPCQSSQ 164
+++ IGTPAQ +DT +D W C C C ST +F+ SSSF LPC S
Sbjct: 95 YLMNLSIGTPAQPFSAIMDTGSDLIWTQCQPCTQCFNQSTPIFNPQGSSSFSTLPCSSQL 154
Query: 165 CNQVPNPSCSGSACGFNLTYGS-STVAADLVQDNLTLATDSVPSYTFGCIRKATG-SSVP 224
C + +P+CS + C + YG S + + LT + S+P+ TFGC G
Sbjct: 155 CQALSSPTCSNNFCQYTYGYGDGSETQGSMGTETLTFGSVSIPNITFGCGENNQGFGQGN 214
Query: 225 PQGLLGLGRGPLSLLGQSQSLYQSTFSYCLPSFKSVNFSGSLRLGPVAQPIRI--KYTPL 284
GL+G+GRGPLSL Q L + FSYC+ S S +L LG +A + T L
Sbjct: 215 GAGLVGMGRGPLSLPSQ---LDVTKFSYCMTPIGSSTPS-NLLLGSLANSVTAGSPNTTL 274
Query: 285 LRNPRRSSLYYVNLISIRVGRKIVDIPPSALAFNSATGAG-TVIDSGTTFTRLVAPAYTA 344
+++ + + YY+ L + VG + I PSA A NS G G +IDSGTT T V AY +
Sbjct: 275 IQSSQIPTFYYITLNGLSVGSTRLPIDPSAFALNSNNGTGGIIIDSGTTLTYFVNNAYQS 334
Query: 345 VRDEFRRRVGRNVTVSSLGGFDTCYTVP-----IISPTITFMFAGMNVTLPPDNFLIHST 404
VR EF ++ V S GFD C+ P + PT F G ++ LP +N+ I S
Sbjct: 335 VRQEFISQINLPVVNGSSSGFDLCFQTPSDPSNLQIPTFVMHFDGGDLELPSENYFI-SP 394
Query: 405 AGSTTCLAMAAAPDNVNSVLNVIASMQQQNHRILFDIPNSRVGVARESCSS 444
+ CLAM ++ +++ ++QQQN +++D NS V A C +
Sbjct: 395 SNGLICLAMGSSSQG----MSIFGNIQQQNMLVVYDTGNSVVSFASAQCGA 436
BLAST of CSPI05G27660 vs. TrEMBL
Match:
A0A0A0KTR5_CUCSA (Aspartic proteinase nepenthesin-1 OS=Cucumis sativus GN=Csa_5G623870 PE=3 SV=1)
HSP 1 Score: 858.2 bits (2216), Expect = 4.4e-246
Identity = 440/443 (99.32%), Postives = 440/443 (99.32%), Query Frame = 1
Query: 1 MNNTKSPFLLLLLLLLLHLFSISTAKSHIPSNCNPAADRSSTLQVFHIFSPCSPFRPSKP 60
MNNTKSPFL LLLLLLHLFSISTAKSHIPSNCNPAADRSSTLQVFHIFSPCSPFRPSKP
Sbjct: 1 MNNTKSPFL--LLLLLLHLFSISTAKSHIPSNCNPAADRSSTLQVFHIFSPCSPFRPSKP 60
Query: 61 LSWADNVLQMQAKDQARLQFLSSLVARRSFVPIASARQLIQSPTFVVRAKIGTPAQTLLL 120
LSWADNVLQMQAKDQARLQFLSSLVARRSFVPIASARQLIQSPTFVVRAKIGTPAQTLLL
Sbjct: 61 LSWADNVLQMQAKDQARLQFLSSLVARRSFVPIASARQLIQSPTFVVRAKIGTPAQTLLL 120
Query: 121 ALDTSNDAAWIPCSGCIGCPSTTVFSSDKSSSFRPLPCQSSQCNQVPNPSCSGSACGFNL 180
ALDTSNDAAWIPCSGCIGCPSTTVFSSDKSSSFRPLPCQS QCNQVPNPSCSGSACGFNL
Sbjct: 121 ALDTSNDAAWIPCSGCIGCPSTTVFSSDKSSSFRPLPCQSPQCNQVPNPSCSGSACGFNL 180
Query: 181 TYGSSTVAADLVQDNLTLATDSVPSYTFGCIRKATGSSVPPQGLLGLGRGPLSLLGQSQS 240
TYGSSTVAADLVQDNLTLATDSVPSYTFGCIRKATGSSVPPQGLLGLGRGPLSLLGQSQS
Sbjct: 181 TYGSSTVAADLVQDNLTLATDSVPSYTFGCIRKATGSSVPPQGLLGLGRGPLSLLGQSQS 240
Query: 241 LYQSTFSYCLPSFKSVNFSGSLRLGPVAQPIRIKYTPLLRNPRRSSLYYVNLISIRVGRK 300
LYQSTFSYCLPSFKSVNFSGSLRLGPVAQPIRIKYTPLLRNPRRSSLYYVNLISIRVGRK
Sbjct: 241 LYQSTFSYCLPSFKSVNFSGSLRLGPVAQPIRIKYTPLLRNPRRSSLYYVNLISIRVGRK 300
Query: 301 IVDIPPSALAFNSATGAGTVIDSGTTFTRLVAPAYTAVRDEFRRRVGRNVTVSSLGGFDT 360
IVDIPPSALAFNSATGAGTVIDSGTTFTRLVAPAYTAVRDEFRRRVGRNVTVSSLGGFDT
Sbjct: 301 IVDIPPSALAFNSATGAGTVIDSGTTFTRLVAPAYTAVRDEFRRRVGRNVTVSSLGGFDT 360
Query: 361 CYTVPIISPTITFMFAGMNVTLPPDNFLIHSTAGSTTCLAMAAAPDNVNSVLNVIASMQQ 420
CYTVPIISPTITFMFAGMNVTLPPDNFLIHSTAGSTTCLAMAAAPDNVNSVLNVIASMQQ
Sbjct: 361 CYTVPIISPTITFMFAGMNVTLPPDNFLIHSTAGSTTCLAMAAAPDNVNSVLNVIASMQQ 420
Query: 421 QNHRILFDIPNSRVGVARESCSS 444
QNHRILFDIPNSRVGVARESCSS
Sbjct: 421 QNHRILFDIPNSRVGVARESCSS 441
BLAST of CSPI05G27660 vs. TrEMBL
Match:
A0A067L7Q3_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_24539 PE=3 SV=1)
HSP 1 Score: 666.0 bits (1717), Expect = 3.2e-188
Identity = 335/430 (77.91%), Postives = 381/430 (88.60%), Query Frame = 1
Query: 13 LLLLLHLFSISTAKSHIPSNCNPAADRSSTLQVFHIFSPCSPFRPSKPLSWADNVLQMQA 72
LL L LF H+ C+ + D+ STLQVFH++SPCSPFRPSKPLSW ++VLQMQA
Sbjct: 5 LLSLAFLFFSLAQGLHLNPKCS-SQDQGSTLQVFHVYSPCSPFRPSKPLSWEESVLQMQA 64
Query: 73 KDQARLQFLSSLVARRSFVPIASARQLIQSPTFVVRAKIGTPAQTLLLALDTSNDAAWIP 132
KDQARLQFLSSLVA RSFVPIAS RQ+IQSPT++VRAKIGTPAQTLLLA+DTSNDAAWIP
Sbjct: 65 KDQARLQFLSSLVAGRSFVPIASGRQIIQSPTYIVRAKIGTPAQTLLLAVDTSNDAAWIP 124
Query: 133 CSGCIGCPSTTVFSSDKSSSFRPLPCQSSQCNQVPNPSCSGSACGFNLTYGSSTVAADLV 192
CSGC GC S+TVF S KS+SF+ + C + QC QVPNP+CSGSAC FN TYGSS++AA+L
Sbjct: 125 CSGCDGC-SSTVFDSVKSTSFQTVGCGAPQCKQVPNPTCSGSACTFNTTYGSSSIAANLS 184
Query: 193 QDNLTLATDSVPSYTFGCIRKATGSSVPPQGLLGLGRGPLSLLGQSQSLYQSTFSYCLPS 252
QD ++LATDSVP YTFGCI KATGSSVPPQGLLGLGRGPLSLL Q+Q+LYQSTFSYCLPS
Sbjct: 185 QDTVSLATDSVPGYTFGCIAKATGSSVPPQGLLGLGRGPLSLLSQTQNLYQSTFSYCLPS 244
Query: 253 FKSVNFSGSLRLGPVAQPIRIKYTPLLRNPRRSSLYYVNLISIRVGRKIVDIPPSALAFN 312
F+S+NFSG+LRLGP QP RIK TPLLRNPRRSSLYYVNL++IRVGR++VDIPPSALAFN
Sbjct: 245 FRSLNFSGTLRLGPNGQPKRIKTTPLLRNPRRSSLYYVNLVAIRVGRRVVDIPPSALAFN 304
Query: 313 SATGAGTVIDSGTTFTRLVAPAYTAVRDEFRRRVGRNVTVSSLGGFDTCYTVPIISPTIT 372
TGAGT+ DSGT FTRLV PAYTAVRD FR+RVG N TV+SLGGFDTCY+VPI++PTIT
Sbjct: 305 PTTGAGTIFDSGTVFTRLVTPAYTAVRDAFRKRVG-NATVTSLGGFDTCYSVPIVAPTIT 364
Query: 373 FMFAGMNVTLPPDNFLIHSTAGSTTCLAMAAAPDNVNSVLNVIASMQQQNHRILFDIPNS 432
FMF+GMNVTLPP+N LIHSTAGST+CLA+AAAPDNVNSVLNVIA+MQQQNHRILFD+PNS
Sbjct: 365 FMFSGMNVTLPPENLLIHSTAGSTSCLAIAAAPDNVNSVLNVIANMQQQNHRILFDVPNS 424
Query: 433 RVGVARESCS 443
R+GVARE C+
Sbjct: 425 RLGVAREQCT 431
BLAST of CSPI05G27660 vs. TrEMBL
Match:
I1JW44_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_04G136000 PE=3 SV=1)
HSP 1 Score: 658.3 bits (1697), Expect = 6.7e-186
Identity = 328/436 (75.23%), Postives = 381/436 (87.39%), Query Frame = 1
Query: 11 LLLLLLLHLFSISTAKSHIPSNCNPAADRSSTLQVFHIFSPCSPFRPSKPLSWADNVLQM 70
L L L LF + + + C+ D STL+VFH+FSPCSPFRP KPLSWA++VLQ+
Sbjct: 5 LFSLSPLFLFLLFSLVEGLTPKCD-TQDHGSTLEVFHVFSPCSPFRPPKPLSWAESVLQL 64
Query: 71 QAKDQARLQFLSSLVARRSFVPIASARQLIQSPTFVVRAKIGTPAQTLLLALDTSNDAAW 130
QAKDQARLQFL+S+VA RS VPIAS RQ+IQSPT++VRAKIG+P QTLLLA+DTSNDAAW
Sbjct: 65 QAKDQARLQFLASMVAGRSVVPIASGRQIIQSPTYIVRAKIGSPPQTLLLAMDTSNDAAW 124
Query: 131 IPCSGCIGCPSTTVFSSDKSSSFRPLPCQSSQCNQVPNPSCSGSACGFNLTYGSSTVAAD 190
IPC+ C GC ST +F+ +KS++F+ + C S QCNQVPNPSC SAC FNLTYGSS++AA+
Sbjct: 125 IPCTACDGCTST-LFAPEKSTTFKNVSCGSPQCNQVPNPSCGTSACTFNLTYGSSSIAAN 184
Query: 191 LVQDNLTLATDSVPSYTFGCIRKATGSSVPPQGLLGLGRGPLSLLGQSQSLYQSTFSYCL 250
+VQD +TLATD +P YTFGC+ K TG+S PPQGLLGLGRGPLSLL Q+Q+LYQSTFSYCL
Sbjct: 185 VVQDTVTLATDPIPDYTFGCVAKTTGASAPPQGLLGLGRGPLSLLSQTQNLYQSTFSYCL 244
Query: 251 PSFKSVNFSGSLRLGPVAQPIRIKYTPLLRNPRRSSLYYVNLISIRVGRKIVDIPPSALA 310
PSFKS+NFSGSLRLGPVAQPIRIKYTPLL+NPRRSSLYYVNL++IRVGRK+VDIPP ALA
Sbjct: 245 PSFKSLNFSGSLRLGPVAQPIRIKYTPLLKNPRRSSLYYVNLVAIRVGRKVVDIPPEALA 304
Query: 311 FNSATGAGTVIDSGTTFTRLVAPAYTAVRDEFRRRVG----RNVTVSSLGGFDTCYTVPI 370
FN+ATGAGTV DSGT FTRLVAPAYTAVRDEF+RRV N+TV+SLGGFDTCYTVPI
Sbjct: 305 FNAATGAGTVFDSGTVFTRLVAPAYTAVRDEFQRRVAIAAKANLTVTSLGGFDTCYTVPI 364
Query: 371 ISPTITFMFAGMNVTLPPDNFLIHSTAGSTTCLAMAAAPDNVNSVLNVIASMQQQNHRIL 430
++PTITFMF+GMNVTLP DN LIHSTAGSTTCLAMA+APDNVNSVLNVIA+MQQQNHR+L
Sbjct: 365 VAPTITFMFSGMNVTLPEDNILIHSTAGSTTCLAMASAPDNVNSVLNVIANMQQQNHRVL 424
Query: 431 FDIPNSRVGVARESCS 443
+D+PNSR+GVARE C+
Sbjct: 425 YDVPNSRLGVARELCT 438
BLAST of CSPI05G27660 vs. TrEMBL
Match:
A0A0B2PGF3_GLYSO (Aspartic proteinase nepenthesin-1 OS=Glycine soja GN=glysoja_045617 PE=3 SV=1)
HSP 1 Score: 658.3 bits (1697), Expect = 6.7e-186
Identity = 328/436 (75.23%), Postives = 381/436 (87.39%), Query Frame = 1
Query: 11 LLLLLLLHLFSISTAKSHIPSNCNPAADRSSTLQVFHIFSPCSPFRPSKPLSWADNVLQM 70
L L L LF + + + C+ D STL+VFH+FSPCSPFRP KPLSWA++VLQ+
Sbjct: 5 LFSLSPLFLFLLFSLVEGLTPKCD-TQDHGSTLEVFHVFSPCSPFRPPKPLSWAESVLQL 64
Query: 71 QAKDQARLQFLSSLVARRSFVPIASARQLIQSPTFVVRAKIGTPAQTLLLALDTSNDAAW 130
QAKDQARLQFL+S+VA RS VPIAS RQ+IQSPT++VRAKIG+P QTLLLA+DTSNDAAW
Sbjct: 65 QAKDQARLQFLASMVAGRSVVPIASGRQIIQSPTYIVRAKIGSPPQTLLLAMDTSNDAAW 124
Query: 131 IPCSGCIGCPSTTVFSSDKSSSFRPLPCQSSQCNQVPNPSCSGSACGFNLTYGSSTVAAD 190
IPC+ C GC ST +F+ +KS++F+ + C S QCNQVPNPSC SAC FNLTYGSS++AA+
Sbjct: 125 IPCTACDGCTST-LFAPEKSTTFKNVSCGSPQCNQVPNPSCGTSACTFNLTYGSSSIAAN 184
Query: 191 LVQDNLTLATDSVPSYTFGCIRKATGSSVPPQGLLGLGRGPLSLLGQSQSLYQSTFSYCL 250
+VQD +TLATD +P YTFGC+ K TG+S PPQGLLGLGRGPLSLL Q+Q+LYQSTFSYCL
Sbjct: 185 VVQDTVTLATDPIPDYTFGCVAKTTGASAPPQGLLGLGRGPLSLLSQTQNLYQSTFSYCL 244
Query: 251 PSFKSVNFSGSLRLGPVAQPIRIKYTPLLRNPRRSSLYYVNLISIRVGRKIVDIPPSALA 310
PSFKS+NFSGSLRLGPVAQPIRIKYTPLL+NPRRSSLYYVNL++IRVGRK+VDIPP ALA
Sbjct: 245 PSFKSLNFSGSLRLGPVAQPIRIKYTPLLKNPRRSSLYYVNLVAIRVGRKVVDIPPEALA 304
Query: 311 FNSATGAGTVIDSGTTFTRLVAPAYTAVRDEFRRRVG----RNVTVSSLGGFDTCYTVPI 370
FN+ATGAGTV DSGT FTRLVAPAYTAVRDEF+RRV N+TV+SLGGFDTCYTVPI
Sbjct: 305 FNAATGAGTVFDSGTVFTRLVAPAYTAVRDEFQRRVAIAAKANLTVTSLGGFDTCYTVPI 364
Query: 371 ISPTITFMFAGMNVTLPPDNFLIHSTAGSTTCLAMAAAPDNVNSVLNVIASMQQQNHRIL 430
++PTITFMF+GMNVTLP DN LIHSTAGSTTCLAMA+APDNVNSVLNVIA+MQQQNHR+L
Sbjct: 365 VAPTITFMFSGMNVTLPEDNILIHSTAGSTTCLAMASAPDNVNSVLNVIANMQQQNHRVL 424
Query: 431 FDIPNSRVGVARESCS 443
+D+PNSR+GVARE C+
Sbjct: 425 YDVPNSRLGVARELCT 438
BLAST of CSPI05G27660 vs. TrEMBL
Match:
V4V1N5_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10001212mg PE=3 SV=1)
HSP 1 Score: 656.8 bits (1693), Expect = 1.9e-185
Identity = 328/434 (75.58%), Postives = 384/434 (88.48%), Query Frame = 1
Query: 10 LLLLLLLLHLFSISTAKSHIPSNCNPAADRSSTLQVFHIFSPCSPFRPSKPLSWADNVLQ 69
L+ L L LFS+S + I C+ D SSTLQVFH+FSPCSPF+PSKPLSW ++VL+
Sbjct: 5 LVFFLAFLFLFSLSEGLNPI---CD-TQDHSSTLQVFHVFSPCSPFKPSKPLSWEESVLE 64
Query: 70 MQAKDQARLQFLSSL-VARRSFVPIASARQLIQSPTFVVRAKIGTPAQTLLLALDTSNDA 129
M AKDQARLQFLSSL VAR+S VPIAS RQ+ QSPT++VRAKIGTPAQTLL+A+DTSNDA
Sbjct: 65 MLAKDQARLQFLSSLAVARKSVVPIASGRQITQSPTYIVRAKIGTPAQTLLMAMDTSNDA 124
Query: 130 AWIPCSGCIGCPSTTVFSSDKSSSFRPLPCQSSQCNQVPNPSCSGSACGFNLTYGSSTVA 189
AW+PC+GC+GC S+TVF+S +S++F+ L CQ++QC QVPNP+C G AC FNLTYGSST+A
Sbjct: 125 AWVPCTGCVGC-SSTVFNSAQSTTFKNLGCQAAQCKQVPNPTCGGGACAFNLTYGSSTIA 184
Query: 190 ADLVQDNLTLATDSVPSYTFGCIRKATGSSVPPQGLLGLGRGPLSLLGQSQSLYQSTFSY 249
A+L QD ++LATD VP YTFGCI+KATG+SVPPQGLLGLGRG LSLL Q+Q+LYQSTFSY
Sbjct: 185 ANLSQDTISLATDIVPGYTFGCIQKATGNSVPPQGLLGLGRGSLSLLAQTQNLYQSTFSY 244
Query: 250 CLPSFKSVNFSGSLRLGPVAQPIRIKYTPLLRNPRRSSLYYVNLISIRVGRKIVDIPPSA 309
CLPSFK+++FSGSLRLGP+ QP RIKYTPLL+NPRRSSLYYVNL++IRVGR++VDIPP A
Sbjct: 245 CLPSFKALSFSGSLRLGPIGQPKRIKYTPLLKNPRRSSLYYVNLLAIRVGRRVVDIPPGA 304
Query: 310 LAFNSATGAGTVIDSGTTFTRLVAPAYTAVRDEFRRRVGRNVTVSSLGGFDTCYTVPIIS 369
L FN TGAGT+IDSGT FTRLVAPAYTAVRD FRRRVG N+TV+SLGGFDTCY+VPI++
Sbjct: 305 LQFNPTTGAGTIIDSGTVFTRLVAPAYTAVRDVFRRRVGSNLTVTSLGGFDTCYSVPIVA 364
Query: 370 PTITFMFAGMNVTLPPDNFLIHSTAGSTTCLAMAAAPDNVNSVLNVIASMQQQNHRILFD 429
PTIT MF+GMNVTLP DN LIHSTAGS TCLAMAAAPDNVNSVLNVIA+MQQQNHRIL+D
Sbjct: 365 PTITLMFSGMNVTLPQDNLLIHSTAGSITCLAMAAAPDNVNSVLNVIANMQQQNHRILYD 424
Query: 430 IPNSRVGVARESCS 443
+PNSR+GVARE C+
Sbjct: 425 VPNSRLGVARELCT 433
BLAST of CSPI05G27660 vs. TAIR10
Match:
AT5G07030.1 (AT5G07030.1 Eukaryotic aspartyl protease family protein)
HSP 1 Score: 577.8 bits (1488), Expect = 5.8e-165
Identity = 286/447 (63.98%), Postives = 354/447 (79.19%), Query Frame = 1
Query: 2 NNTKSPFLLLLLLLLLHLFSI---STAKSHIPSNCNPAADRSSTLQVFHIFSPCSPFRPS 61
+N K+ + L+L L LFSI + +H + D+ STL++FHI SPCSPF+ S
Sbjct: 9 SNPKAYNTMSTLVLFLQLFSILPLALGLNHPNCDLTKTQDQGSTLRIFHIDSPCSPFKSS 68
Query: 62 KPLSWADNVLQMQAKDQARLQFLSSLVARRSFVPIASARQLIQSPTFVVRAKIGTPAQTL 121
PLSW VLQ A+DQARLQ+LSSLVA RS VPIAS RQ++QS T++V+A IGTPAQ L
Sbjct: 69 SPLSWEARVLQTLAQDQARLQYLSSLVAGRSVVPIASGRQMLQSTTYIVKALIGTPAQPL 128
Query: 122 LLALDTSNDAAWIPCSGCIGCPSTTVFSSDKSSSFRPLPCQSSQCNQVPNPSCSGSACGF 181
LLA+DTS+D AWIPCSGC+GCPS T FS KS+SF+ + C + QC QVPNP+C AC F
Sbjct: 129 LLAMDTSSDVAWIPCSGCVGCPSNTAFSPAKSTSFKNVSCSAPQCKQVPNPTCGARACSF 188
Query: 182 NLTYGSSTVAADLVQDNLTLATDSVPSYTFGCIRKATGSSV--PPQGLLGLGRGPLSLLG 241
NLTYGSS++AA+L QD + LA D + ++TFGC+ K G PPQGLLGLGRGPLSL+
Sbjct: 189 NLTYGSSSIAANLSQDTIRLAADPIKAFTFGCVNKVAGGGTIPPPQGLLGLGRGPLSLMS 248
Query: 242 QSQSLYQSTFSYCLPSFKSVNFSGSLRLGPVAQPIRIKYTPLLRNPRRSSLYYVNLISIR 301
Q+QS+Y+STFSYCLPSF+S+ FSGSLRLGP +QP R+KYT LLRNPRRSSLYYVNL++IR
Sbjct: 249 QAQSIYKSTFSYCLPSFRSLTFSGSLRLGPTSQPQRVKYTQLLRNPRRSSLYYVNLVAIR 308
Query: 302 VGRKIVDIPPSALAFNSATGAGTVIDSGTTFTRLVAPAYTAVRDEFRRRV-GRNVTVSSL 361
VGRK+VD+PP+A+AFN +TGAGT+ DSGT +TRL P Y AVR+EFR+RV V+SL
Sbjct: 309 VGRKVVDLPPAAIAFNPSTGAGTIFDSGTVYTRLAKPVYEAVRNEFRKRVKPTTAVVTSL 368
Query: 362 GGFDTCYTVPIISPTITFMFAGMNVTLPPDNFLIHSTAGSTTCLAMAAAPDNVNSVLNVI 421
GGFDTCY+ + PTITFMF G+N+T+P DN ++HSTAGST+CLAMAAAP+NVNSV+NVI
Sbjct: 369 GGFDTCYSGQVKVPTITFMFKGVNMTMPADNLMLHSTAGSTSCLAMAAAPENVNSVVNVI 428
Query: 422 ASMQQQNHRILFDIPNSRVGVARESCS 443
ASMQQQNHR+L D+PN R+G+ARE CS
Sbjct: 429 ASMQQQNHRVLIDVPNGRLGLARERCS 455
BLAST of CSPI05G27660 vs. TAIR10
Match:
AT3G54400.1 (AT3G54400.1 Eukaryotic aspartyl protease family protein)
HSP 1 Score: 563.5 bits (1451), Expect = 1.1e-160
Identity = 295/436 (67.66%), Postives = 353/436 (80.96%), Query Frame = 1
Query: 9 LLLLLLLLLHLFSISTAKSHIPSNCNPAADRSSTLQVFHIFSPCSPFRPSKPLSWADNVL 68
LL+LL+ LL L S S NCN + SS L+VFHI S CSPF+ S +SWAD +L
Sbjct: 5 LLILLISLLILKSESI-------NCNEKS-HSSDLRVFHINSLCSPFKTS--VSWADTLL 64
Query: 69 QMQAKDQARLQFLSSLVA-RRSFVPIASARQLIQSPTFVVRAKIGTPAQTLLLALDTSND 128
Q D+AR +LSSL R+S VPIAS R ++QSPT++VRA IGTPAQ +L+ALDTSND
Sbjct: 65 Q----DKARFLYLSSLAGVRKSSVPIASGRAIVQSPTYIVRANIGTPAQPMLVALDTSND 124
Query: 129 AAWIPCSGCIGCPSTTVFSSDKSSSFRPLPCQSSQCNQVPNPSCSGS-ACGFNLTYGSST 188
AAWIPCSGC+GC S+ +F KSSS R L C++ QC Q PNPSC+ S +CGFN+TYG ST
Sbjct: 125 AAWIPCSGCVGCSSSVLFDPSKSSSSRTLQCEAPQCKQAPNPSCTVSKSCGFNMTYGGST 184
Query: 189 VAADLVQDNLTLATDSVPSYTFGCIRKATGSSVPPQGLLGLGRGPLSLLGQSQSLYQSTF 248
+ A L QD LTLA+D +P+YTFGCI KA+G+S+P QGL+GLGRGPLSL+ QSQ+LYQSTF
Sbjct: 185 IEAYLTQDTLTLASDVIPNYTFGCINKASGTSLPAQGLMGLGRGPLSLISQSQNLYQSTF 244
Query: 249 SYCLPSFKSVNFSGSLRLGPVAQPIRIKYTPLLRNPRRSSLYYVNLISIRVGRKIVDIPP 308
SYCLP+ KS NFSGSLRLGP QPIRIK TPLL+NPRRSSLYYVNL+ IRVG KIVDIP
Sbjct: 245 SYCLPNSKSSNFSGSLRLGPKNQPIRIKTTPLLKNPRRSSLYYVNLVGIRVGNKIVDIPT 304
Query: 309 SALAFNSATGAGTVIDSGTTFTRLVAPAYTAVRDEFRRRVGRNVTVSSLGGFDTCYTVPI 368
SALAF+ ATGAGT+ DSGT +TRLV PAY AVR+EFRRRV +N +SLGGFDTCY+ +
Sbjct: 305 SALAFDPATGAGTIFDSGTVYTRLVEPAYVAVRNEFRRRV-KNANATSLGGFDTCYSGSV 364
Query: 369 ISPTITFMFAGMNVTLPPDNFLIHSTAGSTTCLAMAAAPDNVNSVLNVIASMQQQNHRIL 428
+ P++TFMFAGMNVTLPPDN LIHS+AG+ +CLAMAAAP NVNSVLNVIASMQQQNHR+L
Sbjct: 365 VFPSVTFMFAGMNVTLPPDNLLIHSSAGNLSCLAMAAAPVNVNSVLNVIASMQQQNHRVL 424
Query: 429 FDIPNSRVGVARESCS 443
D+PNSR+G++RE+C+
Sbjct: 425 IDVPNSRLGISRETCT 425
BLAST of CSPI05G27660 vs. TAIR10
Match:
AT1G09750.1 (AT1G09750.1 Eukaryotic aspartyl protease family protein)
HSP 1 Score: 406.0 bits (1042), Expect = 3.0e-113
Identity = 227/456 (49.78%), Postives = 304/456 (66.67%), Query Frame = 1
Query: 1 MNNTKSPFLLLLLLLLLHLFSISTAKSHIPSNCNPAA-DRSSTLQVFHIFSPCSPFRPSK 60
M ++ F L LLL F+ +T + C AA D S L + I + CSPF P+
Sbjct: 1 MASSSLHFFFFLTLLLPFTFTTATRDT-----CATAAPDGSDDLSIIPINAKCSPFAPTH 60
Query: 61 -PLSWADNVLQMQAKDQARLQFLSSLVARR---SFVPIASARQLIQSPTFVVRAKIGTPA 120
S D VL M + D RL +LSSLVA + + VP+AS QL +VVRAK+GTP
Sbjct: 61 VSASVIDTVLHMASSDSHRLTYLSSLVAGKPKPTSVPVASGNQL-HIGNYVVRAKLGTPP 120
Query: 121 QTLLLALDTSNDAAWIPCSGCIGCPS-TTVFSSDKSSSFRPLPCQSSQCNQV-----PNP 180
Q + + LDTSNDA W+PCSGC GC + +T F+++ SS++ + C ++QC Q P+
Sbjct: 121 QLMFMVLDTSNDAVWLPCSGCSGCSNASTSFNTNSSSTYSTVSCSTAQCTQARGLTCPSS 180
Query: 181 SCSGSACGFNLTYGS-STVAADLVQDNLTLATDSVPSYTFGCIRKATGSSVPPQGLLGLG 240
S S C FN +YG S+ +A LVQD LTLA D +P+++FGCI A+G+S+PPQGL+GLG
Sbjct: 181 SPQPSVCSFNQSYGGDSSFSASLVQDTLTLAPDVIPNFSFGCINSASGNSLPPQGLMGLG 240
Query: 241 RGPLSLLGQSQSLYQSTFSYCLPSFKSVNFSGSLRLGPVAQPIRIKYTPLLRNPRRSSLY 300
RGP+SL+ Q+ SLY FSYCLPSF+S FSGSL+LG + QP I+YTPLLRNPRR SLY
Sbjct: 241 RGPMSLVSQTTSLYSGVFSYCLPSFRSFYFSGSLKLGLLGQPKSIRYTPLLRNPRRPSLY 300
Query: 301 YVNLISIRVGRKIVDIPPSALAFNSATGAGTVIDSGTTFTRLVAPAYTAVRDEFRRRVGR 360
YVNL + VG V + P L F++ +GAGT+IDSGT TR P Y A+RDEFR++V
Sbjct: 301 YVNLTGVSVGSVQVPVDPVYLTFDANSGAGTIIDSGTVITRFAQPVYEAIRDEFRKQVNV 360
Query: 361 NVTVSSLGGFDTCYTV--PIISPTITFMFAGMNVTLPPDNFLIHSTAGSTTCLAMAAAPD 420
+ + S+LG FDTC++ ++P IT +++ LP +N LIHS+AG+ TCL+MA
Sbjct: 361 S-SFSTLGAFDTCFSADNENVAPKITLHMTSLDLKLPMENTLIHSSAGTLTCLSMAGIRQ 420
Query: 421 NVNSVLNVIASMQQQNHRILFDIPNSRVGVARESCS 443
N N+VLNVIA++QQQN RILFD+PNSR+G+A E C+
Sbjct: 421 NANAVLNVIANLQQQNLRILFDVPNSRIGIAPEPCN 449
BLAST of CSPI05G27660 vs. TAIR10
Match:
AT1G01300.1 (AT1G01300.1 Eukaryotic aspartyl protease family protein)
HSP 1 Score: 187.2 bits (474), Expect = 2.2e-47
Identity = 132/441 (29.93%), Postives = 208/441 (47.17%), Query Frame = 1
Query: 21 SISTAKSHIPSNCNPAADRSSTLQVFHIFSPCSPFRPSKPLSWADNVLQMQAKDQARLQF 80
S S +S S + + S TL + HI + S P + S + LQ ++ +
Sbjct: 52 SESLLESEFESGSDSESSSSITLNLDHIDALSSNKTPDELFS---SRLQRDSRRVKSIAT 111
Query: 81 LSSLVARRSFV----PIASARQLIQ-----SPTFVVRAKIGTPAQTLLLALDTSNDAAWI 140
L++ + R+ P + ++ S + R +GTPA+ + + LDT +D W+
Sbjct: 112 LAAQIPGRNVTHAPRPGGFSSSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWL 171
Query: 141 PCSGCIGCPSTT--VFSSDKSSSFRPLPCQSSQCNQVPNPSCSG--SACGFNLTYGS-ST 200
C+ C C S + +F KS ++ +PC S C ++ + C+ C + ++YG S
Sbjct: 172 QCAPCRRCYSQSDPIFDPRKSKTYATIPCSSPHCRRLDSAGCNTRRKTCLYQVSYGDGSF 231
Query: 201 VAADLVQDNLTLATDSVPSYTFGCIRKATGSSVPPQGLLGLGRGPLSLLGQSQSLYQSTF 260
D + LT + V GC G V GLLGLG+G LS GQ+ + F
Sbjct: 232 TVGDFSTETLTFRRNRVKGVALGCGHDNEGLFVGAAGLLGLGKGKLSFPGQTGHRFNQKF 291
Query: 261 SYCLPSFKSVNFSGSLRLGPVAQPIRIKYTPLLRNPRRSSLYYVNLISIRVG-RKIVDIP 320
SYCL + + S+ G A ++TPLL NP+ + YYV L+ I VG ++ +
Sbjct: 292 SYCLVDRSASSKPSSVVFGNAAVSRIARFTPLLSNPKLDTFYYVGLLGISVGGTRVPGVT 351
Query: 321 PSALAFNSATGAGTVIDSGTTFTRLVAPAYTAVRDEFRRRVGRNVTVSSLGGFDTCYTVP 380
S + G +IDSGT+ TRL+ PAY A+RD FR FDTC+ +
Sbjct: 352 ASLFKLDQIGNGGVIIDSGTSVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLFDTCFDLS 411
Query: 381 IIS----PTITFMFAGMNVTLPPDNFLIHSTAGSTTCLAMAAAPDNVNSVLNVIASMQQQ 440
++ PT+ F G +V+LP N+LI C A A L++I ++QQQ
Sbjct: 412 NMNEVKVPTVVLHFRGADVSLPATNYLIPVDTNGKFCFAFAGTMGG----LSIIGNIQQQ 471
Query: 441 NHRILFDIPNSRVGVARESCS 443
R+++D+ +SRVG A C+
Sbjct: 472 GFRVVYDLASSRVGFAPGGCA 485
BLAST of CSPI05G27660 vs. TAIR10
Match:
AT3G20015.1 (AT3G20015.1 Eukaryotic aspartyl protease family protein)
HSP 1 Score: 185.7 bits (470), Expect = 6.4e-47
Identity = 117/348 (33.62%), Postives = 167/348 (47.99%), Query Frame = 1
Query: 102 SPTFVVRAKIGTPAQTLLLALDTSNDAAWIPCSGCIGC--PSTTVFSSDKSSSFRPLPCQ 161
S + VR +G+P + + +D+ +D W+ C C C S VF KS S+ + C
Sbjct: 128 SGEYFVRIGVGSPPRDQYMVIDSGSDMVWVQCQPCKLCYKQSDPVFDPAKSGSYTGVSCG 187
Query: 162 SSQCNQVPNPSCSGSACGFNLTYGS-STVAADLVQDNLTLATDSVPSYTFGCIRKATGSS 221
SS C+++ N C C + + YG S L + LT A V + GC + G
Sbjct: 188 SSVCDRIENSGCHSGGCRYEVMYGDGSYTKGTLALETLTFAKTVVRNVAMGCGHRNRGMF 247
Query: 222 VPPQGLLGLGRGPLSLLGQSQSLYQSTFSYCLPSFKSVNFSGSLRLGPVAQPIRIKYTPL 281
+ GLLG+G G +S +GQ F YCL S + + +GSL G A P+ + PL
Sbjct: 248 IGAAGLLGIGGGSMSFVGQLSGQTGGAFGYCLVS-RGTDSTGSLVFGREALPVGASWVPL 307
Query: 282 LRNPRRSSLYYVNLISIRVGRKIVDIPPSALAFNSATGAGTVIDSGTTFTRLVAPAYTAV 341
+RNPR S YYV L + VG + +P G V+D+GT TRL AY A
Sbjct: 308 VRNPRAPSFYYVGLKGLGVGGVRIPLPDGVFDLTETGDGGVVMDTGTAVTRLPTAAYVAF 367
Query: 342 RDEFRRRVGRNVTVSSLGGFDTCYT----VPIISPTITFMFA-GMNVTLPPDNFLIHSTA 401
RD F+ + S + FDTCY V + PT++F F G +TLP NFL+
Sbjct: 368 RDGFKSQTANLPRASGVSIFDTCYDLSGFVSVRVPTVSFYFTEGPVLTLPARNFLMPVDD 427
Query: 402 GSTTCLAMAAAPDNVNSVLNVIASMQQQNHRILFDIPNSRVGVARESC 442
T C A AA+P L++I ++QQ+ ++ FD N VG C
Sbjct: 428 SGTYCFAFAASPTG----LSIIGNIQQEGIQVSFDGANGFVGFGPNVC 470
BLAST of CSPI05G27660 vs. NCBI nr
Match:
gi|449449334|ref|XP_004142420.1| (PREDICTED: protein ASPARTIC PROTEASE IN GUARD CELL 2 [Cucumis sativus])
HSP 1 Score: 858.2 bits (2216), Expect = 6.3e-246
Identity = 440/443 (99.32%), Postives = 440/443 (99.32%), Query Frame = 1
Query: 1 MNNTKSPFLLLLLLLLLHLFSISTAKSHIPSNCNPAADRSSTLQVFHIFSPCSPFRPSKP 60
MNNTKSPFL LLLLLLHLFSISTAKSHIPSNCNPAADRSSTLQVFHIFSPCSPFRPSKP
Sbjct: 1 MNNTKSPFL--LLLLLLHLFSISTAKSHIPSNCNPAADRSSTLQVFHIFSPCSPFRPSKP 60
Query: 61 LSWADNVLQMQAKDQARLQFLSSLVARRSFVPIASARQLIQSPTFVVRAKIGTPAQTLLL 120
LSWADNVLQMQAKDQARLQFLSSLVARRSFVPIASARQLIQSPTFVVRAKIGTPAQTLLL
Sbjct: 61 LSWADNVLQMQAKDQARLQFLSSLVARRSFVPIASARQLIQSPTFVVRAKIGTPAQTLLL 120
Query: 121 ALDTSNDAAWIPCSGCIGCPSTTVFSSDKSSSFRPLPCQSSQCNQVPNPSCSGSACGFNL 180
ALDTSNDAAWIPCSGCIGCPSTTVFSSDKSSSFRPLPCQS QCNQVPNPSCSGSACGFNL
Sbjct: 121 ALDTSNDAAWIPCSGCIGCPSTTVFSSDKSSSFRPLPCQSPQCNQVPNPSCSGSACGFNL 180
Query: 181 TYGSSTVAADLVQDNLTLATDSVPSYTFGCIRKATGSSVPPQGLLGLGRGPLSLLGQSQS 240
TYGSSTVAADLVQDNLTLATDSVPSYTFGCIRKATGSSVPPQGLLGLGRGPLSLLGQSQS
Sbjct: 181 TYGSSTVAADLVQDNLTLATDSVPSYTFGCIRKATGSSVPPQGLLGLGRGPLSLLGQSQS 240
Query: 241 LYQSTFSYCLPSFKSVNFSGSLRLGPVAQPIRIKYTPLLRNPRRSSLYYVNLISIRVGRK 300
LYQSTFSYCLPSFKSVNFSGSLRLGPVAQPIRIKYTPLLRNPRRSSLYYVNLISIRVGRK
Sbjct: 241 LYQSTFSYCLPSFKSVNFSGSLRLGPVAQPIRIKYTPLLRNPRRSSLYYVNLISIRVGRK 300
Query: 301 IVDIPPSALAFNSATGAGTVIDSGTTFTRLVAPAYTAVRDEFRRRVGRNVTVSSLGGFDT 360
IVDIPPSALAFNSATGAGTVIDSGTTFTRLVAPAYTAVRDEFRRRVGRNVTVSSLGGFDT
Sbjct: 301 IVDIPPSALAFNSATGAGTVIDSGTTFTRLVAPAYTAVRDEFRRRVGRNVTVSSLGGFDT 360
Query: 361 CYTVPIISPTITFMFAGMNVTLPPDNFLIHSTAGSTTCLAMAAAPDNVNSVLNVIASMQQ 420
CYTVPIISPTITFMFAGMNVTLPPDNFLIHSTAGSTTCLAMAAAPDNVNSVLNVIASMQQ
Sbjct: 361 CYTVPIISPTITFMFAGMNVTLPPDNFLIHSTAGSTTCLAMAAAPDNVNSVLNVIASMQQ 420
Query: 421 QNHRILFDIPNSRVGVARESCSS 444
QNHRILFDIPNSRVGVARESCSS
Sbjct: 421 QNHRILFDIPNSRVGVARESCSS 441
BLAST of CSPI05G27660 vs. NCBI nr
Match:
gi|659092233|ref|XP_008446966.1| (PREDICTED: aspartic proteinase nepenthesin-1 [Cucumis melo])
HSP 1 Score: 834.7 bits (2155), Expect = 7.4e-239
Identity = 427/444 (96.17%), Postives = 436/444 (98.20%), Query Frame = 1
Query: 1 MNNTKSPFLLL-LLLLLLHLFSISTAKSHIPSNCNPAADRSSTLQVFHIFSPCSPFRPSK 60
MNNTKSPFL L LLLLLL LFSISTAKSHIP NCNPA DRSSTL+VFHIFSPCSPFRPSK
Sbjct: 1 MNNTKSPFLPLPLLLLLLLLFSISTAKSHIPLNCNPADDRSSTLKVFHIFSPCSPFRPSK 60
Query: 61 PLSWADNVLQMQAKDQARLQFLSSLVARRSFVPIASARQLIQSPTFVVRAKIGTPAQTLL 120
PLSWADNVLQMQAKDQARLQFLSSLVARRS VPIASARQLIQSPTFVVRAKIGTPAQTLL
Sbjct: 61 PLSWADNVLQMQAKDQARLQFLSSLVARRSVVPIASARQLIQSPTFVVRAKIGTPAQTLL 120
Query: 121 LALDTSNDAAWIPCSGCIGCPSTTVFSSDKSSSFRPLPCQSSQCNQVPNPSCSGSACGFN 180
LALDTSNDAAWIPCSGC+GCPSTTVFSSDKSSSFRPLPCQS QCNQVPNPSCSG+ACGFN
Sbjct: 121 LALDTSNDAAWIPCSGCVGCPSTTVFSSDKSSSFRPLPCQSPQCNQVPNPSCSGTACGFN 180
Query: 181 LTYGSSTVAADLVQDNLTLATDSVPSYTFGCIRKATGSSVPPQGLLGLGRGPLSLLGQSQ 240
LTYGSSTVAADLVQDN+TLATDSVP+YTFGCIRKATGSSVPPQGLLGLGRGPLSLLGQSQ
Sbjct: 181 LTYGSSTVAADLVQDNVTLATDSVPAYTFGCIRKATGSSVPPQGLLGLGRGPLSLLGQSQ 240
Query: 241 SLYQSTFSYCLPSFKSVNFSGSLRLGPVAQPIRIKYTPLLRNPRRSSLYYVNLISIRVGR 300
SLY+STFSYCLPSFKSVNFSGSLRLGPVAQPIRIKYTPLLRNPRRSSLYYVNLI+IRVGR
Sbjct: 241 SLYRSTFSYCLPSFKSVNFSGSLRLGPVAQPIRIKYTPLLRNPRRSSLYYVNLIAIRVGR 300
Query: 301 KIVDIPPSALAFNSATGAGTVIDSGTTFTRLVAPAYTAVRDEFRRRVGRNVTVSSLGGFD 360
+IVDIPPSALAFNSATGAGT+IDSGTTFTRLVAPAYTAVRDEFRRRVGRNVTVSSLGGFD
Sbjct: 301 RIVDIPPSALAFNSATGAGTIIDSGTTFTRLVAPAYTAVRDEFRRRVGRNVTVSSLGGFD 360
Query: 361 TCYTVPIISPTITFMFAGMNVTLPPDNFLIHSTAGSTTCLAMAAAPDNVNSVLNVIASMQ 420
TCYTVPIISPTITFMFAGMNVTLPPDNFLIHSTAGSTTCLAMAAAPDNVNSVLNVIASMQ
Sbjct: 361 TCYTVPIISPTITFMFAGMNVTLPPDNFLIHSTAGSTTCLAMAAAPDNVNSVLNVIASMQ 420
Query: 421 QQNHRILFDIPNSRVGVARESCSS 444
QQNHRILFDIPNSRVGVARE CSS
Sbjct: 421 QQNHRILFDIPNSRVGVAREPCSS 444
BLAST of CSPI05G27660 vs. NCBI nr
Match:
gi|802574128|ref|XP_012068673.1| (PREDICTED: protein ASPARTIC PROTEASE IN GUARD CELL 2 [Jatropha curcas])
HSP 1 Score: 666.0 bits (1717), Expect = 4.6e-188
Identity = 335/430 (77.91%), Postives = 381/430 (88.60%), Query Frame = 1
Query: 13 LLLLLHLFSISTAKSHIPSNCNPAADRSSTLQVFHIFSPCSPFRPSKPLSWADNVLQMQA 72
LL L LF H+ C+ + D+ STLQVFH++SPCSPFRPSKPLSW ++VLQMQA
Sbjct: 5 LLSLAFLFFSLAQGLHLNPKCS-SQDQGSTLQVFHVYSPCSPFRPSKPLSWEESVLQMQA 64
Query: 73 KDQARLQFLSSLVARRSFVPIASARQLIQSPTFVVRAKIGTPAQTLLLALDTSNDAAWIP 132
KDQARLQFLSSLVA RSFVPIAS RQ+IQSPT++VRAKIGTPAQTLLLA+DTSNDAAWIP
Sbjct: 65 KDQARLQFLSSLVAGRSFVPIASGRQIIQSPTYIVRAKIGTPAQTLLLAVDTSNDAAWIP 124
Query: 133 CSGCIGCPSTTVFSSDKSSSFRPLPCQSSQCNQVPNPSCSGSACGFNLTYGSSTVAADLV 192
CSGC GC S+TVF S KS+SF+ + C + QC QVPNP+CSGSAC FN TYGSS++AA+L
Sbjct: 125 CSGCDGC-SSTVFDSVKSTSFQTVGCGAPQCKQVPNPTCSGSACTFNTTYGSSSIAANLS 184
Query: 193 QDNLTLATDSVPSYTFGCIRKATGSSVPPQGLLGLGRGPLSLLGQSQSLYQSTFSYCLPS 252
QD ++LATDSVP YTFGCI KATGSSVPPQGLLGLGRGPLSLL Q+Q+LYQSTFSYCLPS
Sbjct: 185 QDTVSLATDSVPGYTFGCIAKATGSSVPPQGLLGLGRGPLSLLSQTQNLYQSTFSYCLPS 244
Query: 253 FKSVNFSGSLRLGPVAQPIRIKYTPLLRNPRRSSLYYVNLISIRVGRKIVDIPPSALAFN 312
F+S+NFSG+LRLGP QP RIK TPLLRNPRRSSLYYVNL++IRVGR++VDIPPSALAFN
Sbjct: 245 FRSLNFSGTLRLGPNGQPKRIKTTPLLRNPRRSSLYYVNLVAIRVGRRVVDIPPSALAFN 304
Query: 313 SATGAGTVIDSGTTFTRLVAPAYTAVRDEFRRRVGRNVTVSSLGGFDTCYTVPIISPTIT 372
TGAGT+ DSGT FTRLV PAYTAVRD FR+RVG N TV+SLGGFDTCY+VPI++PTIT
Sbjct: 305 PTTGAGTIFDSGTVFTRLVTPAYTAVRDAFRKRVG-NATVTSLGGFDTCYSVPIVAPTIT 364
Query: 373 FMFAGMNVTLPPDNFLIHSTAGSTTCLAMAAAPDNVNSVLNVIASMQQQNHRILFDIPNS 432
FMF+GMNVTLPP+N LIHSTAGST+CLA+AAAPDNVNSVLNVIA+MQQQNHRILFD+PNS
Sbjct: 365 FMFSGMNVTLPPENLLIHSTAGSTSCLAIAAAPDNVNSVLNVIANMQQQNHRILFDVPNS 424
Query: 433 RVGVARESCS 443
R+GVARE C+
Sbjct: 425 RLGVAREQCT 431
BLAST of CSPI05G27660 vs. NCBI nr
Match:
gi|1009171231|ref|XP_015866632.1| (PREDICTED: protein ASPARTIC PROTEASE IN GUARD CELL 2-like [Ziziphus jujuba])
HSP 1 Score: 662.5 bits (1708), Expect = 5.1e-187
Identity = 323/421 (76.72%), Postives = 372/421 (88.36%), Query Frame = 1
Query: 22 ISTAKSHIPSNCNPAADRSSTLQVFHIFSPCSPFRPSKPLSWADNVLQMQAKDQARLQFL 81
I+T + NC D+ STLQV H++SPCSPFRPSKPLSW ++VLQMQAKDQARLQFL
Sbjct: 17 ITTIVQGLNLNCQNQ-DKGSTLQVLHVYSPCSPFRPSKPLSWEESVLQMQAKDQARLQFL 76
Query: 82 SSLVARRSFVPIASARQLIQSPTFVVRAKIGTPAQTLLLALDTSNDAAWIPCSGCIGCPS 141
SSLVAR+S VPIAS RQ+IQSPT++VRAKIGTP QTLLLALDTSNDAAWIPC+GC+GC S
Sbjct: 77 SSLVARKSVVPIASGRQIIQSPTYIVRAKIGTPVQTLLLALDTSNDAAWIPCAGCVGC-S 136
Query: 142 TTVFSSDKSSSFRPLPCQSSQCNQVPNPSCSGSACGFNLTYGSSTVAADLVQDNLTLATD 201
+ F+ KS+SF+ L CQ+ QC QVPNP+C+G+ C FNLTYG S++AADL QD +TLA D
Sbjct: 137 SAAFAPIKSTSFKSLGCQAPQCRQVPNPTCTGTTCSFNLTYGGSSIAADLSQDTITLAND 196
Query: 202 SVPSYTFGCIRKATGSSVPPQGLLGLGRGPLSLLGQSQSLYQSTFSYCLPSFKSVNFSGS 261
+VP+YTFGCI+KATGSS+PPQGLLGLGRGPLSLL Q+Q+LYQSTFSYCLPSFKS+NFSGS
Sbjct: 197 AVPAYTFGCIKKATGSSLPPQGLLGLGRGPLSLLSQTQNLYQSTFSYCLPSFKSLNFSGS 256
Query: 262 LRLGPVAQPIRIKYTPLLRNPRRSSLYYVNLISIRVGRKIVDIPPSALAFNSATGAGTVI 321
LRLGP+ QPIRIKYTPLL+NPRRSSLYYVNL +IRVGRK+VDIPP+ LAFN TGAGT+
Sbjct: 257 LRLGPIGQPIRIKYTPLLKNPRRSSLYYVNLFAIRVGRKVVDIPPADLAFNPTTGAGTIF 316
Query: 322 DSGTTFTRLVAPAYTAVRDEFRRRVGRNVTVSSLGGFDTCYTVPIISPTITFMFAGMNVT 381
DSGT FTRLVA AYTAVRDEFR+RV N +++LGGFDTCY+VPI +PT+TFMF GMNVT
Sbjct: 317 DSGTVFTRLVASAYTAVRDEFRKRVKPNAPITTLGGFDTCYSVPITAPTVTFMFTGMNVT 376
Query: 382 LPPDNFLIHSTAGSTTCLAMAAAPDNVNSVLNVIASMQQQNHRILFDIPNSRVGVARESC 441
LPPDN LIHSTAGS TCLAMAAAPDNVNSVLNVIA+MQQQNHRIL+D+PNSR+GVARE C
Sbjct: 377 LPPDNLLIHSTAGSITCLAMAAAPDNVNSVLNVIANMQQQNHRILYDVPNSRLGVAREPC 435
Query: 442 S 443
+
Sbjct: 437 T 435
BLAST of CSPI05G27660 vs. NCBI nr
Match:
gi|356508308|ref|XP_003522900.1| (PREDICTED: protein ASPARTIC PROTEASE IN GUARD CELL 2-like [Glycine max])
HSP 1 Score: 658.3 bits (1697), Expect = 9.5e-186
Identity = 328/436 (75.23%), Postives = 381/436 (87.39%), Query Frame = 1
Query: 11 LLLLLLLHLFSISTAKSHIPSNCNPAADRSSTLQVFHIFSPCSPFRPSKPLSWADNVLQM 70
L L L LF + + + C+ D STL+VFH+FSPCSPFRP KPLSWA++VLQ+
Sbjct: 5 LFSLSPLFLFLLFSLVEGLTPKCD-TQDHGSTLEVFHVFSPCSPFRPPKPLSWAESVLQL 64
Query: 71 QAKDQARLQFLSSLVARRSFVPIASARQLIQSPTFVVRAKIGTPAQTLLLALDTSNDAAW 130
QAKDQARLQFL+S+VA RS VPIAS RQ+IQSPT++VRAKIG+P QTLLLA+DTSNDAAW
Sbjct: 65 QAKDQARLQFLASMVAGRSVVPIASGRQIIQSPTYIVRAKIGSPPQTLLLAMDTSNDAAW 124
Query: 131 IPCSGCIGCPSTTVFSSDKSSSFRPLPCQSSQCNQVPNPSCSGSACGFNLTYGSSTVAAD 190
IPC+ C GC ST +F+ +KS++F+ + C S QCNQVPNPSC SAC FNLTYGSS++AA+
Sbjct: 125 IPCTACDGCTST-LFAPEKSTTFKNVSCGSPQCNQVPNPSCGTSACTFNLTYGSSSIAAN 184
Query: 191 LVQDNLTLATDSVPSYTFGCIRKATGSSVPPQGLLGLGRGPLSLLGQSQSLYQSTFSYCL 250
+VQD +TLATD +P YTFGC+ K TG+S PPQGLLGLGRGPLSLL Q+Q+LYQSTFSYCL
Sbjct: 185 VVQDTVTLATDPIPDYTFGCVAKTTGASAPPQGLLGLGRGPLSLLSQTQNLYQSTFSYCL 244
Query: 251 PSFKSVNFSGSLRLGPVAQPIRIKYTPLLRNPRRSSLYYVNLISIRVGRKIVDIPPSALA 310
PSFKS+NFSGSLRLGPVAQPIRIKYTPLL+NPRRSSLYYVNL++IRVGRK+VDIPP ALA
Sbjct: 245 PSFKSLNFSGSLRLGPVAQPIRIKYTPLLKNPRRSSLYYVNLVAIRVGRKVVDIPPEALA 304
Query: 311 FNSATGAGTVIDSGTTFTRLVAPAYTAVRDEFRRRVG----RNVTVSSLGGFDTCYTVPI 370
FN+ATGAGTV DSGT FTRLVAPAYTAVRDEF+RRV N+TV+SLGGFDTCYTVPI
Sbjct: 305 FNAATGAGTVFDSGTVFTRLVAPAYTAVRDEFQRRVAIAAKANLTVTSLGGFDTCYTVPI 364
Query: 371 ISPTITFMFAGMNVTLPPDNFLIHSTAGSTTCLAMAAAPDNVNSVLNVIASMQQQNHRIL 430
++PTITFMF+GMNVTLP DN LIHSTAGSTTCLAMA+APDNVNSVLNVIA+MQQQNHR+L
Sbjct: 365 VAPTITFMFSGMNVTLPEDNILIHSTAGSTTCLAMASAPDNVNSVLNVIANMQQQNHRVL 424
Query: 431 FDIPNSRVGVARESCS 443
+D+PNSR+GVARE C+
Sbjct: 425 YDVPNSRLGVARELCT 438
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| AED3_ARATH | 5.3e-112 | 49.78 | Aspartyl protease AED3 OS=Arabidopsis thaliana GN=AED3 PE=1 SV=1 | [more] |
| AP25_ORYSJ | 4.0e-99 | 48.14 | Aspartyl protease 25 OS=Oryza sativa subsp. japonica GN=AP25 PE=2 SV=1 | [more] |
| APF2_ARATH | 3.9e-46 | 29.93 | Aspartyl protease family protein 2 OS=Arabidopsis thaliana GN=APF2 PE=2 SV=1 | [more] |
| ASPG2_ARATH | 1.1e-45 | 33.62 | Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana GN=ASPG2 PE=2 ... | [more] |
| NEP1_NEPGR | 1.3e-44 | 35.04 | Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis GN=nep1 PE=1 SV=1 | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A0A0KTR5_CUCSA | 4.4e-246 | 99.32 | Aspartic proteinase nepenthesin-1 OS=Cucumis sativus GN=Csa_5G623870 PE=3 SV=1 | [more] |
| A0A067L7Q3_JATCU | 3.2e-188 | 77.91 | Uncharacterized protein OS=Jatropha curcas GN=JCGZ_24539 PE=3 SV=1 | [more] |
| I1JW44_SOYBN | 6.7e-186 | 75.23 | Uncharacterized protein OS=Glycine max GN=GLYMA_04G136000 PE=3 SV=1 | [more] |
| A0A0B2PGF3_GLYSO | 6.7e-186 | 75.23 | Aspartic proteinase nepenthesin-1 OS=Glycine soja GN=glysoja_045617 PE=3 SV=1 | [more] |
| V4V1N5_9ROSI | 1.9e-185 | 75.58 | Uncharacterized protein OS=Citrus clementina GN=CICLE_v10001212mg PE=3 SV=1 | [more] |
