CSPI05G21980 (gene) Wild cucumber (PI 183967) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CSPI05G21980
Type	gene
Organism	Cucumis sativus (Wild cucumber (PI 183967))
Description	Cytochrome P450, putative
Location	Chr5 : 22543564 .. 22545132 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTCCTCACATTCGGCTCTGTCCCAACCGTCATCATTTTTTCCACAACAGTTGCAAAAGAGTTGTTCAAACACCATGATCTTGCTTCTTGTAGCCGCCCTTGCTTAGCGGCCACTGCAAAATATTCATACAACTTCTTAGACTTGATTTTTTCCTCTTACGACGACCATTGGAAGGAGCTTCGTAAGATTTGTATTGCTGAGCTCTTTTGTTCCAAACGTGTACAATCTTTTCAACATATTTGAGAAGAGGAAGTGAATCAACTTATGAACTCCATCTCTCAATCCTCATCTTCTTCAACTCTATTTGATTTTACAGCAAAATCATATTCTCTCACGGCTAATATCTTGACTAGGATTGCATTTGGGAAGAGTATTAGAGAGAGTGAAAGTGAACTAAATGATGGTGATGTTGAGGGGGTTATACAAAGAGCGAGTGCGGCAATTGGAAGCTTCTCGGCAAGTGATTTCTTTCCTAGTTTTAGGTGGATTATTGATCGGCTCACCGGTGTTCATGGACGGCTTGAGAAAACCTTTGAGGAGTTGGATGCTTTTTTAGAACATGTAATTGAGGATCGTATTAACTTTAGGACAGTTTGTCGAAACGAAGAAAACATATTCTTGATGTTTTGTTGAGAATGGAGAGAGATTGCTATGAATTTGGTTCAATCAAATTCACTCGAGATTGCATTAAGTCAATTGTCATGGTAAATCTTAAATTTCTTATGCATAACACATAGTAATTTCATCTCAAAATGTGGAATCGTTCTAAATTTTTGAATTCAGATTCTCTAACATGACCCAACAACATCCATTAGTTTTGGTATAGTAAAGGTTTTATTTCATACTTATTGTTTGTTTTAAAAAATAAAAAAATAATAATTTACAATTAAATTTGGAAAAAAAATTCTTTTTTTTTTTTTTTTAAATGTGAAATGCAAACAAGTAACACAAGAGAGGTTACAAAATCAATTTTTTAAACTTTAGCATATCAAGGTAAGTAATTCTTGTGTAACTTAGTTTAACTACCGTTCGAATGTAATAGTAATTGTATGAATTGTAGTTCAAATCACCCTATTCCCAACTGTACGTAGGTGTCTACATATTCACTTTTTTTCTTTAAGAAACTGATATTGTACAATCATGTATGTTTAAGTTTTTTCAACTGGCATGATCAAAGACCAAATCTTCCTGTTTAGTAGTGTGAAAAGAAAAAGTAATTTTCATGTGTTCGTTTTCTTTTGGTGTGTGTGATTTAAGGATTTATTTCTAGCTGGAGTGGAAACAGGAGCAAACACACTTGTTCGAACAATGATAGAGCTAGTTAGAAATTCAAAAGTAATGAAGAAGCTACAACATGAAATCAGAAGCACCATAATTAAACAAGATCAAGTGAAGGAGAACGAGCTCGAAAAGCTTCAATATCTAAAATTGGTGGTGAAAGAGGCTTTAAGGCTTCATCCACCCGTCCCAATGTTACTTCCAAGAGAATGCATGGGCAATTGGAAGAGACACGGATTCTTGGAAAAACCTAGAAGAGTTTTATCCAGAAAGGTTTATAAAAAGTAA

mRNA sequence

ATGCTCCTCACATTCGGCTCTGTCCCAACCGTCATCATTTTTTCCACAACAGTTGCAAAAGAGTTGTTCAAACACCATGATCTTGCTTCTTGTAGCCGCCCTTGCTTAGCGGCCACTGCAAAATATTCATACAACTTCTTAGACTTGATTTTTTCCTCTTACGACGACCATTGGAAGGAGCTTCCAAAATCATATTCTCTCACGGCTAATATCTTGACTAGGATTGCATTTGGGAAGAGTATTAGAGAGAGTGAAAGTGAACTAAATGATGGTGATGTTGAGGGGGTTATACAAAGAGCGAGTGCGGCAATTGGAAGCTTCTCGGCAAGTGATTTCTTTCCTAGTTTTAGGTGGATTATTGATCGGCTCACCGGTGTTCATGGACGGCTTGAGAAAACCTTTGAGGAGTTGGATGCTTTTTTAGAACATGATTTATTTCTAGCTGGAGTGGAAACAGGAGCAAACACACTTGTTCGAACAATGATAGAGCTAGTTAGAAATTCAAAAGTAATGAAGAAGCTACAACATGAAATCAGAAGCACCATAATTAAACAAGATCAAGTGAAGGAGAACGAGCTCGAAAAGCTTCAATATCTAAAATTGGTGGTGAAAGAGGCTTTAAGGCTTCATCCACCCGTCCCAATGTTACTTCCAAGAGAATGCATGGGCAATTGGAAGAGACACGGATTCTTGGAAAAACCTAGAAGAGTTTTATCCAGAAAGGTTTATAAAAAGTAA

Coding sequence (CDS)

ATGCTCCTCACATTCGGCTCTGTCCCAACCGTCATCATTTTTTCCACAACAGTTGCAAAAGAGTTGTTCAAACACCATGATCTTGCTTCTTGTAGCCGCCCTTGCTTAGCGGCCACTGCAAAATATTCATACAACTTCTTAGACTTGATTTTTTCCTCTTACGACGACCATTGGAAGGAGCTTCCAAAATCATATTCTCTCACGGCTAATATCTTGACTAGGATTGCATTTGGGAAGAGTATTAGAGAGAGTGAAAGTGAACTAAATGATGGTGATGTTGAGGGGGTTATACAAAGAGCGAGTGCGGCAATTGGAAGCTTCTCGGCAAGTGATTTCTTTCCTAGTTTTAGGTGGATTATTGATCGGCTCACCGGTGTTCATGGACGGCTTGAGAAAACCTTTGAGGAGTTGGATGCTTTTTTAGAACATGATTTATTTCTAGCTGGAGTGGAAACAGGAGCAAACACACTTGTTCGAACAATGATAGAGCTAGTTAGAAATTCAAAAGTAATGAAGAAGCTACAACATGAAATCAGAAGCACCATAATTAAACAAGATCAAGTGAAGGAGAACGAGCTCGAAAAGCTTCAATATCTAAAATTGGTGGTGAAAGAGGCTTTAAGGCTTCATCCACCCGTCCCAATGTTACTTCCAAGAGAATGCATGGGCAATTGGAAGAGACACGGATTCTTGGAAAAACCTAGAAGAGTTTTATCCAGAAAGGTTTATAAAAAGTAA

Homology

[Close]

BLAST of CSPI05G21980 vs. Swiss-Prot
Match: C71BY_ARATH (Cytochrome P450 71B37 OS=Arabidopsis thaliana GN=CYP71B37 PE=3 SV=2)

HSP 1 Score: 134.8 bits (338), Expect = 1.3e-30
Identity = 97/320 (30.31%), Postives = 148/320 (46.25%), Query Frame = 1


Query: 1   MLLTFGSVPTVIIFSTTVAKELFKHHDLASCSRPCLAATAKYSYNFLDLIFSSYDDHWKE 60
           MLL FGS+PTV++ S+  AK+  K HDL  CSRP LA     SYN+LD++FS ++D+WKE
Sbjct: 66  MLLKFGSIPTVVVSSSETAKQALKIHDLNCCSRPSLAGPRALSYNYLDIVFSPFNDYWKE 125

Query: 61  L---------------------------------------------PKSYSLTANILTRI 120
           L                                              K  SLT  ++ + 
Sbjct: 126 LRRMCVQELFSPKQVHLIQPIREEEVKKLMNSFSESAAQKTPVNLSEKLASLTVGVICKA 185

Query: 121 AFGKSIRESESELNDGDVEGVIQRASAAIGSFSASDFFPSFRWIIDRLTGVHGRLEKTFE 180
           AFG S + +   LN  + + +I  A   +GSFSASD+FP+  WIID LTG+ G+ E++  
Sbjct: 186 AFGVSFQGTV--LNSDNFDKLIHDAFLFLGSFSASDYFPNVGWIIDWLTGLQGQRERSVR 245

Query: 181 ELDAFLEH--DLFLAGVETGANTLVRTMIELVRNSKVM---KKLQHEIRSTIIK------ 231
            LDAF E   DL   G + G    V  +++L +   V+   K  ++ I++ ++       
Sbjct: 246 GLDAFYEQMFDLHKQGNKEGVEDFVDLLLKLEKEETVLGYGKLTRNHIKAVLMNVLLGGI 305

[Close]

BLAST of CSPI05G21980 vs. Swiss-Prot
Match: C71BE_ARATH (Cytochrome P450 71B14 OS=Arabidopsis thaliana GN=CYP71B14 PE=2 SV=1)

HSP 1 Score: 113.6 bits (283), Expect = 3.0e-24
Identity = 68/212 (32.08%), Postives = 109/212 (51.42%), Query Frame = 1


Query: 63  KSYSLTANILTRIAFGKSIRESESELNDGDVEGVIQRASAAIGSFSASDFFPSFRWIIDR 122
           K   L+ +++ ++ FG S+  S+        E VIQ     +G F+A+D+FP    IIDR
Sbjct: 171 KLVKLSGSVICKVGFGISLEWSKLA---NTYEEVIQGTMEVVGRFAAADYFPIIGRIIDR 230

Query: 123 LTGVHGRLEKTFEELDAF----LEHDL--------------------------------- 182
           +TG+H + EK F+E+D+F    ++H L                                 
Sbjct: 231 ITGLHSKCEKVFKEMDSFFDQSIKHHLEDTNIKDDIIGLLLKMEKGETGLGEFQLTRNHT 290

Query: 183 -------FLAGVETGANTLVRTMIELVRNSKVMKKLQHEIRSTIIKQDQVKENELEKLQY 231
                   +AGV+T  +T+   M  L++N +VMKK Q E+R  I  +D + E ++E+L+Y
Sbjct: 291 KGILLNVLIAGVDTSGHTVTWVMTHLIKNPRVMKKAQAEVREVIKNKDDITEEDIERLEY 350

[Close]

BLAST of CSPI05G21980 vs. Swiss-Prot
Match: C71B2_ARATH (Cytochrome P450 71B2 OS=Arabidopsis thaliana GN=CYP71B2 PE=2 SV=2)

HSP 1 Score: 109.0 bits (271), Expect = 7.5e-23
Identity = 68/226 (30.09%), Postives = 118/226 (52.21%), Query Frame = 1


Query: 65  YSLTANILTRIAFGKSIRESESELNDGDVEGVIQRASAAIGSFSASDFFPS--------- 124
           +SLTA+I+ R+A G++  ES   ++   +E ++  ++ A+G+F+ SDFFP          
Sbjct: 175 FSLTASIICRVALGQNFNESGFVIDQDRIEELVTESAEALGTFTFSDFFPGGLGRFVDWL 234

Query: 125 -----------------FRWIIDRLTGVHGRLEKTF-----------EELDAF------- 184
                            ++ +ID      GR  +             E+ D+F       
Sbjct: 235 FQRHKKINKVFKELDAFYQHVIDDHLKPEGRKNQDIVTLILDMIDKQEDSDSFKLNMDNL 294

Query: 185 --LEHDLFLAGVETGANTLVRTMIELVRNSKVMKKLQHEIRSTI-IKQDQVKENELEKLQ 244
             +  D+FLAG++T A T++  M EL+RN +VMKK Q  IR+T+ +K++++ E +L K++
Sbjct: 295 KAIVMDVFLAGIDTSAVTMIWAMTELIRNPRVMKKAQESIRTTLGLKKERITEEDLGKVE 354

[Close]

BLAST of CSPI05G21980 vs. Swiss-Prot
Match: C71B1_THLAR (Cytochrome P450 71B1 OS=Thlaspi arvense GN=CYP71B1 PE=2 SV=1)

HSP 1 Score: 101.7 bits (252), Expect = 1.2e-20
Identity = 66/212 (31.13%), Postives = 104/212 (49.06%), Query Frame = 1


Query: 63  KSYSLTANILTRIAFGKSIRESESELNDGDVEGVIQRASAAIGSFSASDFFPSFRWIIDR 122
           K   ++ +++ RI FG ++  S+ E      + +I +A   +GS +A D+FP    IIDR
Sbjct: 171 KILKMSGSVICRIGFGINLEGSKLE---NTYQEIIVQAFEVLGSLAAVDYFPVIGTIIDR 230

Query: 123 LTGVHGRLEKTFEELDAFLEH----------------DLFL------------------- 182
           +TG+H + EK F  +D+F +                 DL L                   
Sbjct: 231 ITGLHAKCEKVFHGIDSFFDQAIQRHIDDPSIKDDIIDLLLKMERGEGSLGEYELTREHT 290

Query: 183 ---------AGVETGANTLVRTMIELVRNSKVMKKLQHEIRSTIIKQDQVKENELEKLQY 231
                    AG++T A T+   M  L+ N +VMKKLQ EIR  I   D++ ++++E+L Y
Sbjct: 291 KGILMNILTAGIDTSAQTMTWAMTHLLANPRVMKKLQAEIREKIKNIDEITDDDVEQLDY 350

[Close]

BLAST of CSPI05G21980 vs. Swiss-Prot
Match: C71BP_ARATH (Cytochrome P450 71B25 OS=Arabidopsis thaliana GN=CYP71B25 PE=2 SV=1)

HSP 1 Score: 100.9 bits (250), Expect = 2.0e-20
Identity = 59/125 (47.20%), Postives = 81/125 (64.80%), Query Frame = 1


Query: 115 SFRWIIDRLTGVHGRLEKTFEELDAFLEHDLFLAGVETGANTLVRTMIELVRNSKVMKKL 174
           SF+  ID L G+                 D+FLAG++T A T++  M ELV N +VMKK+
Sbjct: 287 SFKLTIDHLKGI---------------TQDIFLAGIDTSAITMIWAMAELVNNPRVMKKV 346

Query: 175 QHEIRSTI-IKQDQVKENELEKLQYLKLVVKEALRLHPPVPMLLPRECMGNWKRHGFLEK 234
           Q EIRS I IK+++++E ++ KLQYLKLV+KE LRLHP  P+LLPRE M + K  G+ + 
Sbjct: 347 QDEIRSCIGIKKERIEEEDVGKLQYLKLVIKETLRLHPAAPLLLPRETMADIKIQGY-DI 395

Query: 235 PRRVL 239
           PR+ L
Sbjct: 407 PRKTL 395

[Close]

BLAST of CSPI05G21980 vs. TrEMBL
Match: A0A0D3AW12_BRAOL (Uncharacterized protein OS=Brassica oleracea var. oleracea PE=3 SV=1)

HSP 1 Score: 169.1 bits (427), Expect = 6.8e-39
Identity = 102/303 (33.66%), Postives = 154/303 (50.83%), Query Frame = 1


Query: 9   PTVIIFSTTVAKELFKHHDLASCSRPCLAATAKYSYNFLDLIFSSYDDHWKEL------- 68
           P+  + S+  AK+  K HDL  CSRP LA   + SYN+LD++FS YDD+WKEL       
Sbjct: 30  PSPPVSSSETAKQALKIHDLHCCSRPSLAGPRELSYNYLDIVFSPYDDYWKELRRMCVQE 89

Query: 69  --------------------------------------PKSYSLTANILTRIAFGKSIRE 128
                                                  K  +L  +++ + AFG S + 
Sbjct: 90  LFSAKRVHLIQPIKDEEVKKLIDTVKESASQGSPVNLSEKFLALNVSVICKAAFGLSFQG 149

Query: 129 SESELNDGDVEGVIQRASAAIGSFSASDFFPSFRWIIDRLTGVHGRLEKTFEELDAFLEH 188
           +   LN+   + +I+ A   +GSFSASDFFP+  WIIDRLTG+ GR EK+  +LDAF E 
Sbjct: 150 TV--LNNDSFDKLIREAFLFLGSFSASDFFPNGGWIIDRLTGLQGRREKSVRDLDAFYEQ 209

Query: 189 ----------------------------DLFLAGVETGANTLVRTMIELVRNSKVMKKLQ 239
                                       ++ L  + T A T+   M EL+RN + MKK+Q
Sbjct: 210 MFDLHKHENKDGVEDFVDLLLRLEKGETNVLLGAISTSAITMTWAMAELMRNPRAMKKVQ 269

[Close]

BLAST of CSPI05G21980 vs. TrEMBL
Match: A0A078EF26_BRANA (BnaC02g36040D protein OS=Brassica napus GN=BnaC02g36040D PE=3 SV=1)

HSP 1 Score: 159.8 bits (403), Expect = 4.1e-36
Identity = 104/314 (33.12%), Postives = 154/314 (49.04%), Query Frame = 1


Query: 9   PTVIIFSTTVAKELFKHHDLASCSRPCLAATAKYSYNFLDLIFSSYDDHWKEL------- 68
           P+  + S+  AK+  K HDL  CSRP LA   + SYN+LD++FS YDD+WKEL       
Sbjct: 30  PSPPVSSSETAKQALKIHDLHCCSRPSLAGPRELSYNYLDIVFSPYDDYWKELRRMCVQE 89

Query: 69  --------------------------------------PKSYSLTANILTRIAFGKSIRE 128
                                                  K  +L  +++ + AFG S + 
Sbjct: 90  LFSAKRVHLIQPIKDEEVKKLIDTVKESASQGSPVNLSEKFLALNVSVICKAAFGLSFQG 149

Query: 129 SESELNDGDVEGVIQRASAAIGSFSASDFFPSFRWIIDRLTGVHGRLEKTFEELDAFLEH 188
           +   LN+   + +I+ A   +GSFSASDFFP+  WIIDRLTG+ GR EK+  +LDAF E 
Sbjct: 150 TV--LNNDSFDKLIREAFLFLGSFSASDFFPNGGWIIDRLTGLQGRREKSVRDLDAFYEQ 209

Query: 189 DLFL------AGVETGANTLVR---------------------------------TMIEL 239
              L       GVE   + L+R                                  M EL
Sbjct: 210 MFDLHKHENKDGVEDFVDLLLRLEKGETLTRNHIKAILMNVLLGAISTSAITMTWAMAEL 269

[Close]

BLAST of CSPI05G21980 vs. TrEMBL
Match: A0A067KEL5_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_11117 PE=3 SV=1)

HSP 1 Score: 155.6 bits (392), Expect = 7.8e-35
Identity = 93/243 (38.27%), Postives = 140/243 (57.61%), Query Frame = 1


Query: 1   MLLTFGSVPTVIIFSTTVAKELFKHHDLASCSRPCLAATAKYSYNFLDLIFSSYDDHWKE 60
           MLL FG VPTVII S   AKELFK +DL SCSRP LA T + SY++LD+    Y D+W+E
Sbjct: 67  MLLQFGRVPTVIISSAEAAKELFKANDLNSCSRPRLAGTGRLSYSYLDIALIPYGDYWRE 126

Query: 61  LPKSYSLTANILTRIAFGKSIRESESELNDGDVEGVIQRASAAIGSFSASDFFPSFRWII 120
           +     L      R+   +SIRE E       ++ +++ +SA+      S+   S    +
Sbjct: 127 MRNICVLQLFSAKRVQSFQSIREEEVRFL---IDSILKSSSASSSPVHLSEKTLSLTANV 186

Query: 121 DRLTGVHGRLEKTFEELDAFLEHDLFLAGVETGANTLVRTMIELVRNSKVMKKLQHEIRS 180
                     ++     + F E ++F+AGV+TGA  LV  M ELVRN + M+K Q EIR+
Sbjct: 187 TCRVAFGESFQERGLAHERFQE-NIFIAGVDTGAIVLVWAMAELVRNPRAMRKAQEEIRT 246

Query: 181 TIIKQDQVKENELEKLQYLKLVVKEALRLHPPVPMLLPRECMGNWKRHGFLEKPRRVLSR 240
            +  + +V E +++KL+YLK+V+KE +RLHPP  +L+PRE M  +  +G+   P+  +  
Sbjct: 247 LLGNKTKVSETDIDKLEYLKMVLKETMRLHPPGTLLIPRETMSQFSINGYEIHPKTRIQV 305

Query: 241 KVY 244
            V+
Sbjct: 307 NVW 305

[Close]

BLAST of CSPI05G21980 vs. TrEMBL
Match: A0A067K354_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_17833 PE=3 SV=1)

HSP 1 Score: 153.7 bits (387), Expect = 2.9e-34
Identity = 116/335 (34.63%), Postives = 162/335 (48.36%), Query Frame = 1


Query: 1   MLLTFGSVPTVIIFSTTVAKELFKHHDLASCSRPCLAATAKY------------------ 60
           MLL FGSVP VII S   A+E  K HDL+ CSRP LA  A+                   
Sbjct: 69  MLLQFGSVPAVIISSAEAAEEALKIHDLSCCSRPPLAGAARLSYNYLDVAFSPYGDYWRE 128

Query: 61  ----------------SYNFLD------LIFSSYDDHWKELP-----KSYSLTANILTRI 120
                           S+ F+       LI S         P     K  +LTANI  R+
Sbjct: 129 IRRICVLELFSTKRVQSFQFVREEEVGLLINSISQSSSSATPVDLTEKFLTLTANITFRM 188

Query: 121 AFGKSIRESESELNDGDVEGVIQRASAAIGSFSASDFFPSFRWIIDRLTG---------- 180
           AFG S    E++ +    + +I  A A +GSFSA++FFP   WIIDR++G          
Sbjct: 189 AFGTSF--GETDFHKDRFKKLIDDAQALLGSFSANEFFPYVGWIIDRISGYHARTENVFC 248

Query: 181 --------------VHGRLEKTFEEL-DAFL----------------EH------DLFLA 240
                           GR +K  E++ D  L                +H      DLFLA
Sbjct: 249 QLDTFFQWIIDDHLKLGRTDKGQEDIIDVLLRIEREKTGVGSVQLTKDHIKAVLMDLFLA 308

Query: 241 GVETGANTLVRTMIELVRNSKVMKKLQHEIRSTIIKQDQVKENELEKLQYLKLVVKEALR 244
           GV TGA TL+  M EL R+ +VMKK Q E+R+ I  +++V E+++++L Y+K+V+KE LR
Sbjct: 309 GVNTGAVTLIWAMAELARSPRVMKKAQEEVRNVIRNKERVLESDVDELYYIKMVIKETLR 368

[Close]

BLAST of CSPI05G21980 vs. TrEMBL
Match: A0A0A0KQ37_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G594450 PE=4 SV=1)

HSP 1 Score: 149.4 bits (376), Expect = 5.6e-33
Identity = 74/81 (91.36%), Postives = 76/81 (93.83%), Query Frame = 1


Query: 63  KSYSLTANILTRIAFGKSIRESESELNDGDVEGVIQRASAAIGSFSASDFFPSFRWIIDR 122
           KSYSLTANILTRI FGKSIRES+SEL+D DVEGVIQRASAAIG FSASDFFPSF WIIDR
Sbjct: 181 KSYSLTANILTRIVFGKSIRESKSELDDSDVEGVIQRASAAIGCFSASDFFPSFGWIIDR 240

Query: 123 LTGVHGRLEKTFEELDAFLEH 144
           LTGVHGRLEK FEELDAFLEH
Sbjct: 241 LTGVHGRLEKNFEELDAFLEH 261

[Close]

BLAST of CSPI05G21980 vs. TAIR10
Match: AT3G26330.1 (AT3G26330.1 cytochrome P450, family 71, subfamily B, polypeptide 37)

HSP 1 Score: 134.8 bits (338), Expect = 7.2e-32
Identity = 97/320 (30.31%), Postives = 148/320 (46.25%), Query Frame = 1


Query: 1   MLLTFGSVPTVIIFSTTVAKELFKHHDLASCSRPCLAATAKYSYNFLDLIFSSYDDHWKE 60
           MLL FGS+PTV++ S+  AK+  K HDL  CSRP LA     SYN+LD++FS ++D+WKE
Sbjct: 66  MLLKFGSIPTVVVSSSETAKQALKIHDLNCCSRPSLAGPRALSYNYLDIVFSPFNDYWKE 125

Query: 61  L---------------------------------------------PKSYSLTANILTRI 120
           L                                              K  SLT  ++ + 
Sbjct: 126 LRRMCVQELFSPKQVHLIQPIREEEVKKLMNSFSESAAQKTPVNLSEKLASLTVGVICKA 185

Query: 121 AFGKSIRESESELNDGDVEGVIQRASAAIGSFSASDFFPSFRWIIDRLTGVHGRLEKTFE 180
           AFG S + +   LN  + + +I  A   +GSFSASD+FP+  WIID LTG+ G+ E++  
Sbjct: 186 AFGVSFQGTV--LNSDNFDKLIHDAFLFLGSFSASDYFPNVGWIIDWLTGLQGQRERSVR 245

Query: 181 ELDAFLEH--DLFLAGVETGANTLVRTMIELVRNSKVM---KKLQHEIRSTIIK------ 231
            LDAF E   DL   G + G    V  +++L +   V+   K  ++ I++ ++       
Sbjct: 246 GLDAFYEQMFDLHKQGNKEGVEDFVDLLLKLEKEETVLGYGKLTRNHIKAVLMNVLLGGI 305

[Close]

BLAST of CSPI05G21980 vs. TAIR10
Match: AT5G25180.1 (AT5G25180.1 cytochrome P450, family 71, subfamily B, polypeptide 14)

HSP 1 Score: 113.6 bits (283), Expect = 1.7e-25
Identity = 68/212 (32.08%), Postives = 109/212 (51.42%), Query Frame = 1


Query: 63  KSYSLTANILTRIAFGKSIRESESELNDGDVEGVIQRASAAIGSFSASDFFPSFRWIIDR 122
           K   L+ +++ ++ FG S+  S+        E VIQ     +G F+A+D+FP    IIDR
Sbjct: 171 KLVKLSGSVICKVGFGISLEWSKLA---NTYEEVIQGTMEVVGRFAAADYFPIIGRIIDR 230

Query: 123 LTGVHGRLEKTFEELDAF----LEHDL--------------------------------- 182
           +TG+H + EK F+E+D+F    ++H L                                 
Sbjct: 231 ITGLHSKCEKVFKEMDSFFDQSIKHHLEDTNIKDDIIGLLLKMEKGETGLGEFQLTRNHT 290

Query: 183 -------FLAGVETGANTLVRTMIELVRNSKVMKKLQHEIRSTIIKQDQVKENELEKLQY 231
                   +AGV+T  +T+   M  L++N +VMKK Q E+R  I  +D + E ++E+L+Y
Sbjct: 291 KGILLNVLIAGVDTSGHTVTWVMTHLIKNPRVMKKAQAEVREVIKNKDDITEEDIERLEY 350

[Close]

BLAST of CSPI05G21980 vs. TAIR10
Match: AT1G13080.1 (AT1G13080.1 cytochrome P450, family 71, subfamily B, polypeptide 2)

HSP 1 Score: 109.0 bits (271), Expect = 4.2e-24
Identity = 68/226 (30.09%), Postives = 118/226 (52.21%), Query Frame = 1


Query: 65  YSLTANILTRIAFGKSIRESESELNDGDVEGVIQRASAAIGSFSASDFFPS--------- 124
           +SLTA+I+ R+A G++  ES   ++   +E ++  ++ A+G+F+ SDFFP          
Sbjct: 175 FSLTASIICRVALGQNFNESGFVIDQDRIEELVTESAEALGTFTFSDFFPGGLGRFVDWL 234

Query: 125 -----------------FRWIIDRLTGVHGRLEKTF-----------EELDAF------- 184
                            ++ +ID      GR  +             E+ D+F       
Sbjct: 235 FQRHKKINKVFKELDAFYQHVIDDHLKPEGRKNQDIVTLILDMIDKQEDSDSFKLNMDNL 294

Query: 185 --LEHDLFLAGVETGANTLVRTMIELVRNSKVMKKLQHEIRSTI-IKQDQVKENELEKLQ 244
             +  D+FLAG++T A T++  M EL+RN +VMKK Q  IR+T+ +K++++ E +L K++
Sbjct: 295 KAIVMDVFLAGIDTSAVTMIWAMTELIRNPRVMKKAQESIRTTLGLKKERITEEDLGKVE 354

[Close]

BLAST of CSPI05G21980 vs. TAIR10
Match: AT3G26270.1 (AT3G26270.1 cytochrome P450, family 71, subfamily B, polypeptide 25)

HSP 1 Score: 100.9 bits (250), Expect = 1.1e-21
Identity = 59/125 (47.20%), Postives = 81/125 (64.80%), Query Frame = 1


Query: 115 SFRWIIDRLTGVHGRLEKTFEELDAFLEHDLFLAGVETGANTLVRTMIELVRNSKVMKKL 174
           SF+  ID L G+                 D+FLAG++T A T++  M ELV N +VMKK+
Sbjct: 287 SFKLTIDHLKGI---------------TQDIFLAGIDTSAITMIWAMAELVNNPRVMKKV 346

Query: 175 QHEIRSTI-IKQDQVKENELEKLQYLKLVVKEALRLHPPVPMLLPRECMGNWKRHGFLEK 234
           Q EIRS I IK+++++E ++ KLQYLKLV+KE LRLHP  P+LLPRE M + K  G+ + 
Sbjct: 347 QDEIRSCIGIKKERIEEEDVGKLQYLKLVIKETLRLHPAAPLLLPRETMADIKIQGY-DI 395

Query: 235 PRRVL 239
           PR+ L
Sbjct: 407 PRKTL 395

[Close]

BLAST of CSPI05G21980 vs. TAIR10
Match: AT5G25130.1 (AT5G25130.1 cytochrome P450, family 71, subfamily B, polypeptide 12)

HSP 1 Score: 100.9 bits (250), Expect = 1.1e-21
Identity = 65/212 (30.66%), Postives = 104/212 (49.06%), Query Frame = 1


Query: 63  KSYSLTANILTRIAFGKSIRESESELNDGDVEGVIQRASAAIGSFSASDFFPSFRWIIDR 122
           K   L+ +++ R+AFG +++ S+ E      E VIQ     +GSF+A+D+FP    IIDR
Sbjct: 171 KLMKLSGSVICRVAFGINLQGSKLE---NTYEEVIQGTVELVGSFAAADYFPVVGRIIDR 230

Query: 123 LTGVHGRLE--------------------------------------KTFEELDAFLEH- 182
           +TG+H + E                                       T  E     +H 
Sbjct: 231 ITGLHSKCEKLFKAMDAFFDQSIKHHLEDEIIKDDIIDLLLKMERGETTLGEFQLTRDHT 290

Query: 183 -----DLFLAGVETGANTLVRTMIELVRNSKVMKKLQHEIRSTIIKQDQVKENELEKLQY 231
                ++  AG++T A  +   M  L+ N +V+KK Q E+R  I  +D + E ++E+LQY
Sbjct: 291 KGILANILNAGIDTSAQVMTWVMTYLISNPRVLKKAQAEVREVIKHKDDIIEEDIERLQY 350

[Close]

BLAST of CSPI05G21980 vs. NCBI nr
Match: gi|778704855|ref|XP_004135281.2| (PREDICTED: cytochrome P450 71B2-like isoform X1 [Cucumis sativus])

HSP 1 Score: 181.8 bits (460), Expect = 1.5e-42
Identity = 107/229 (46.72%), Postives = 139/229 (60.70%), Query Frame = 1


Query: 63  KSYSLTANILTRIAFGKSIRESESELNDGDVEGVIQRASAAIGSFSASDFFPSFRWIIDR 122
           KSYSLTANI TRIAFGKS R  E  L++ + + V++R   AI  FS SDFFPSF WI+DR
Sbjct: 183 KSYSLTANITTRIAFGKSFRGGE--LDNQNFQKVMRRTIDAIKGFSISDFFPSFGWIVDR 242

Query: 123 LTGVHGRLEKTFEELDAFLEH--------------------------------------- 182
           ++GVHG+LEK+F E+DAF +                                        
Sbjct: 243 ISGVHGKLEKSFGEMDAFFQKVVDDRINMDKATSGNEENIVDVLLRMKRDGFQSDALILT 302

Query: 183 ---------DLFLAGVETGANTLVRTMIELVRNSKVMKKLQHEIRSTIIKQDQVKENELE 242
                    D+F+AGVETGANT+V  M EL+R  +VMKKLQ +IRS  IK++QVKE +LE
Sbjct: 303 QDCIKAIIKDIFIAGVETGANTIVWAMAELIRKPRVMKKLQDQIRS-YIKKEQVKETDLE 362

Query: 243 KLQYLKLVVKEALRLHPPVPMLLPRECMGNWKRHGFLEKPRRVLSRKVY 244
           +L YLK+VVKE LRLHPPVP+L+PRE   ++K +G+   P+  L   V+
Sbjct: 363 RLPYLKMVVKEVLRLHPPVPLLIPRETTSHFKLNGYDIHPKAHLHVNVW 408

[Close]

BLAST of CSPI05G21980 vs. NCBI nr
Match: gi|659092302|ref|XP_008447001.1| (PREDICTED: cytochrome P450 71B34-like [Cucumis melo])

HSP 1 Score: 176.8 bits (447), Expect = 4.7e-41
Identity = 110/256 (42.97%), Postives = 148/256 (57.81%), Query Frame = 1


Query: 36  LAATAKYSYNFLDLIFSSYDDHWKELPKSYSLTANILTRIAFGKSIRESESELNDGDVEG 95
           L+A+ K     + + +S   +      KSYSLTANI T+IAFGKS R  E  L++ + + 
Sbjct: 491 LSASKKLPLQLIPIRYSISSNPIDLSDKSYSLTANITTKIAFGKSFRGGE--LDNQNFQK 550

Query: 96  VIQRASAAIGSFSASDFFPSFRWIIDRLTGVHGRLEKTFEELDA---------------- 155
           V++RA  AI SFS +DFFP F WI+DR++GVHG+LEK+F E+DA                
Sbjct: 551 VMRRAVDAIKSFSITDFFPRFGWIVDRISGVHGKLEKSFGEMDAFFQKVVDDRMNFHNAT 610

Query: 156 --------------------------------FLEHDLFLAGVETGANTLVRTMIELVRN 215
                                            L  D+FLAGV+TGA T+V  M+EL+RN
Sbjct: 611 SGNEENIVDVLLKMKRDGFQSDELILTQDCIKALIMDIFLAGVDTGAGTIVWAMVELIRN 670

Query: 216 SKVMKKLQHEIRSTIIKQDQVKENELEKLQYLKLVVKEALRLHPPVPMLLPRECMGNWKR 244
            +VMKKLQ  IRS I K+DQVKE +LE+L YLK+VVKE LRLH P P+LLPRE   ++K 
Sbjct: 671 PRVMKKLQDHIRSHI-KKDQVKEMDLERLPYLKMVVKEVLRLHSPAPLLLPRETTSHFKL 730

[Close]

BLAST of CSPI05G21980 vs. NCBI nr
Match: gi|778704874|ref|XP_004135495.2| (PREDICTED: cytochrome P450 71B37-like [Cucumis sativus])

HSP 1 Score: 176.0 bits (445), Expect = 8.0e-41
Identity = 104/229 (45.41%), Postives = 135/229 (58.95%), Query Frame = 1


Query: 63  KSYSLTANILTRIAFGKSIRESESELNDGDVEGVIQRASAAIGSFSASDFFPSFRWIIDR 122
           KSYSLTANI TRIAFGKS R  E  L++ +   V+ R +  + SFS +DFFPSF WI+DR
Sbjct: 194 KSYSLTANITTRIAFGKSFRGGE--LDNQNFRKVMHRITGVLKSFSITDFFPSFGWIVDR 253

Query: 123 LTGVHGRLEKTFEELDAFLEH--------------------------------------- 182
           ++GVHG+LEK+F E+DAF +                                        
Sbjct: 254 ISGVHGKLEKSFGEMDAFFQKVVDDRINMDKATSGNEENIVDVLLRMKRDGFQFDELVLT 313

Query: 183 ---------DLFLAGVETGANTLVRTMIELVRNSKVMKKLQHEIRSTIIKQDQVKENELE 242
                    D+FLAGVETGA T+V  M EL+RN ++MK+LQ  IRS I K+DQVKE +LE
Sbjct: 314 QDCIKALIMDIFLAGVETGATTIVWAMTELIRNPRIMKRLQTHIRSHI-KKDQVKEKDLE 373

Query: 243 KLQYLKLVVKEALRLHPPVPMLLPRECMGNWKRHGFLEKPRRVLSRKVY 244
           +L YLK+VVKE LRLHPP P+LL RE   ++K +G+   P+  L   V+
Sbjct: 374 RLPYLKMVVKEVLRLHPPAPLLLSREITSHFKLNGYDIHPKAHLHVNVW 419

[Close]

BLAST of CSPI05G21980 vs. NCBI nr
Match: gi|778704869|ref|XP_004135497.2| (PREDICTED: cytochrome P450 71B37-like [Cucumis sativus])

HSP 1 Score: 174.1 bits (440), Expect = 3.0e-40
Identity = 109/220 (49.55%), Postives = 132/220 (60.00%), Query Frame = 1


Query: 63  KSYSLTANILTRIAFGKSIRESESELNDGDVEGVIQRASAAIGSFSASDFFPSFRWIIDR 122
           KSYSLTANILTRI FGKSIRES+SEL+D DVEGVIQ+AS A+G FSASDFFPSF WIIDR
Sbjct: 178 KSYSLTANILTRIVFGKSIRESKSELDDSDVEGVIQKASMAMGRFSASDFFPSFGWIIDR 237

Query: 123 LTGVHGRLEKTFEELDAFLEHDL-----FLAGVETGANTL------------------VR 182
           LTGVH +LEK F+ELDAFLEH +     F A  +   N L                   R
Sbjct: 238 LTGVHEQLEKNFQELDAFLEHVIEDRINFRAACQKEENILDVLLRMERDCYEFGSIKFTR 297

Query: 183 TMIELVRNSKVMKKLQHEIRSTIIKQDQ------------------------VKENELEK 236
             I+ V  +  +  ++    + +    +                        VKENELEK
Sbjct: 298 DCIKAVGMNLFLAGVETGANTLVWTMTELVKNSKIMKKLQHEIRSTIIQQDQVKENELEK 357

[Close]

BLAST of CSPI05G21980 vs. NCBI nr
Match: gi|659090669|ref|XP_008446138.1| (PREDICTED: cytochrome P450 71B37-like [Cucumis melo])

HSP 1 Score: 173.7 bits (439), Expect = 4.0e-40
Identity = 110/188 (58.51%), Postives = 116/188 (61.70%), Query Frame = 1


Query: 1   MLLTFGSVPTVIIFSTTVAKELFKHHDLASCSRPCLAATA-------------------- 60
           MLL FG VPTVII S  VAKELFK HDLASCSRP LAATA                    
Sbjct: 94  MLLKFGPVPTVIISSAMVAKELFKRHDLASCSRPRLAATAKYSYNFLDLIFSSYDDRWRE 153

Query: 61  ------------KYSYNF-----------LDLIF--SSYDDHWKELPKSYSLTANILTRI 120
                       K   +F           L+ I   SS    +    K+YSLT NILTRI
Sbjct: 154 LRKICIAELFSPKRVQSFQHIREEEVNQLLNAISQSSSSSTLFNFTAKTYSLTTNILTRI 213

Query: 121 AFGKSIRESESELNDGDVEGVIQRASAAIGSFSASDFFPSFRWIIDRLTGVHGRLEKTFE 144
           AFGKSIRESES+L+D DVE VIQRASAAIGSFSASD FPSF WIIDRLTGVHGRLEK  E
Sbjct: 214 AFGKSIRESESKLDDCDVERVIQRASAAIGSFSASDLFPSFGWIIDRLTGVHGRLEKNVE 273

The following BLAST results are available for this feature:

BLAST of CSPI05G21980 vs. Swiss-Prot


Match Name	E-value	Identity	Description
C71BY_ARATH	1.3e-30	30.31	Cytochrome P450 71B37 OS=Arabidopsis thaliana GN=CYP71B37 PE=3 SV=2	[more]
C71BE_ARATH	3.0e-24	32.08	Cytochrome P450 71B14 OS=Arabidopsis thaliana GN=CYP71B14 PE=2 SV=1	[more]
C71B2_ARATH	7.5e-23	30.09	Cytochrome P450 71B2 OS=Arabidopsis thaliana GN=CYP71B2 PE=2 SV=2	[more]
C71B1_THLAR	1.2e-20	31.13	Cytochrome P450 71B1 OS=Thlaspi arvense GN=CYP71B1 PE=2 SV=1	[more]
C71BP_ARATH	2.0e-20	47.20	Cytochrome P450 71B25 OS=Arabidopsis thaliana GN=CYP71B25 PE=2 SV=1	[more]

BLAST of CSPI05G21980 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0D3AW12_BRAOL	6.8e-39	33.66	Uncharacterized protein OS=Brassica oleracea var. oleracea PE=3 SV=1	[more]
A0A078EF26_BRANA	4.1e-36	33.12	BnaC02g36040D protein OS=Brassica napus GN=BnaC02g36040D PE=3 SV=1	[more]
A0A067KEL5_JATCU	7.8e-35	38.27	Uncharacterized protein OS=Jatropha curcas GN=JCGZ_11117 PE=3 SV=1	[more]
A0A067K354_JATCU	2.9e-34	34.63	Uncharacterized protein OS=Jatropha curcas GN=JCGZ_17833 PE=3 SV=1	[more]
A0A0A0KQ37_CUCSA	5.6e-33	91.36	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G594450 PE=4 SV=1	[more]

BLAST of CSPI05G21980 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G26330.1	7.2e-32	30.31	cytochrome P450, family 71, subfamily B, polypeptide 37	[more]
AT5G25180.1	1.7e-25	32.08	cytochrome P450, family 71, subfamily B, polypeptide 14	[more]
AT1G13080.1	4.2e-24	30.09	cytochrome P450, family 71, subfamily B, polypeptide 2	[more]
AT3G26270.1	1.1e-21	47.20	cytochrome P450, family 71, subfamily B, polypeptide 25	[more]
AT5G25130.1	1.1e-21	30.66	cytochrome P450, family 71, subfamily B, polypeptide 12	[more]

BLAST of CSPI05G21980 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778704855\|ref\|XP_004135281.2\|	1.5e-42	46.72	PREDICTED: cytochrome P450 71B2-like isoform X1 [Cucumis sativus]	[more]
gi\|659092302\|ref\|XP_008447001.1\|	4.7e-41	42.97	PREDICTED: cytochrome P450 71B34-like [Cucumis melo]	[more]
gi\|778704874\|ref\|XP_004135495.2\|	8.0e-41	45.41	PREDICTED: cytochrome P450 71B37-like [Cucumis sativus]	[more]
gi\|778704869\|ref\|XP_004135497.2\|	3.0e-40	49.55	PREDICTED: cytochrome P450 71B37-like [Cucumis sativus]	[more]
gi\|659090669\|ref\|XP_008446138.1\|	4.0e-40	58.51	PREDICTED: cytochrome P450 71B37-like [Cucumis melo]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR001128	Cyt_P450

Vocabulary: **Molecular Function**
Term	Definition
GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
GO:0005506	iron ion binding
GO:0020037	heme binding

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	oxidation-reduction process

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055114	oxidation-reduction process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
molecular_function	GO:0004497	monooxygenase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI05G21980.1	CSPI05G21980.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	GENE3D	G3DSA:1.10.630.10		coord: 3..61 score: 6.1E-5coord: 66..142 score: 1.7E-6coord: 144..226 score: 1.9
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 144..224 score: 4.7
IPR001128	Cytochrome P450	unknown	SSF48264	Cytochrome P450	coord: 2..230 score: 6.29
None	No IPR available	PANTHER	PTHR24298	FAMILY NOT NAMED	coord: 1..222 score: 2.1
None	No IPR available	PANTHER	PTHR24298:SF144	BIFUNCTIONAL DIHYDROCAMALEXATE SYNTHASE/CAMALEXIN SYNTHASE-RELATED	coord: 1..222 score: 2.1

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CSPI05G21980	Lsi04G006240	Bottle gourd (USVL1VR-Ls)	cpilsiB372
CSPI05G21980	MELO3C011931.2	Melon (DHL92) v3.6.1	cpimedB363
CSPI05G21980	CsaV3_5G030840	Cucumber (Chinese Long) v3	cpicucB284
CSPI05G21980	Cucsa.286700	Cucumber (Gy14) v1	cgycpiB432

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CSPI05G21980	Wild cucumber (PI 183967)	cpicpiB076
CSPI05G21980	Cucumber (Gy14) v1	cgycpiB580
CSPI05G21980	Cucurbita maxima (Rimu)	cmacpiB419
CSPI05G21980	Cucurbita maxima (Rimu)	cmacpiB758
CSPI05G21980	Cucurbita maxima (Rimu)	cmacpiB814
CSPI05G21980	Cucurbita moschata (Rifu)	cmocpiB407
CSPI05G21980	Cucurbita moschata (Rifu)	cmocpiB748
CSPI05G21980	Cucurbita moschata (Rifu)	cmocpiB797
CSPI05G21980	Cucumber (Chinese Long) v2	cpicuB220
CSPI05G21980	Cucumber (Chinese Long) v2	cpicuB239
CSPI05G21980	Melon (DHL92) v3.5.1	cpimeB369
CSPI05G21980	Melon (DHL92) v3.5.1	cpimeB389
CSPI05G21980	Watermelon (Charleston Gray)	cpiwcgB412
CSPI05G21980	Watermelon (Charleston Gray)	cpiwcgB429
CSPI05G21980	Watermelon (97103) v1	cpiwmB382
CSPI05G21980	Watermelon (97103) v1	cpiwmB410
CSPI05G21980	Cucurbita pepo (Zucchini)	cpecpiB042
CSPI05G21980	Cucurbita pepo (Zucchini)	cpecpiB116
CSPI05G21980	Cucurbita pepo (Zucchini)	cpecpiB440
CSPI05G21980	Bottle gourd (USVL1VR-Ls)	cpilsiB392
CSPI05G21980	Melon (DHL92) v3.6.1	cpimedB383
CSPI05G21980	Cucumber (Gy14) v2	cgybcpiB077
CSPI05G21980	Cucumber (Gy14) v2	cgybcpiB225
CSPI05G21980	Silver-seed gourd	carcpiB0275
CSPI05G21980	Silver-seed gourd	carcpiB0538
CSPI05G21980	Cucumber (Chinese Long) v3	cpicucB261
CSPI05G21980	Watermelon (97103) v2	cpiwmbB402
CSPI05G21980	Watermelon (97103) v2	cpiwmbB421
CSPI05G21980	Wax gourd	cpiwgoB516