CSPI05G21810 (gene) Wild cucumber (PI 183967)

Overview
NameCSPI05G21810
Typegene
OrganismCucumis sativus (Wild cucumber (PI 183967))
DescriptionVery-long-chain 3-oxoacyl-CoA reductase-like protein At1g24470
LocationChr5 : 22425242 .. 22426890 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGGAGACATTCTTTCTGCTACTTTCCACTAGTCCCATTTGGCTTCTCATCCTCTCCTTTCTCGGTTTCTTCACCATTCTTTGGCACTCCGCCACTGTCGCCCGATGGGTTTACCGAGCCTTTCTCAGACCCTCCAAGGATCTGAGATACTATGGCTCTTGGGGAATAGTCACTGGCGCCACTGACGGCATTGGAAAGTCCTTTGCCTATCAGCTGGCCAGAGCCGGACTCAACCTTGTACTGGTGAGCAGAAGCTCCATGAAGCTTAAGGCAGTTTCTAAGGATATTCAATCTGAATTTCCTGATACAAAGGTAAAGATAATTGAATTAGACTTCACAGAAGACGATATTAGCGGTGGTATTGCAGAGATTGAGGAGGTGATTGAAGATTTAGATGTGGGGATTTTGATAAACAACGTGGGGATTACATATCCGAACGCTAGCTTTTTCCATGAAGTGGACGAGAAAGTTTGGATGAATGTTTTCAAAGTGAATGTGAAGGGAACTACTTGGGTTACTAAAGTTGTTTTGCCAAAGATGATTAAGAAGAACAGAGGAGCTATTGTGAATATTGGCTCTGGAGCTGCTGTTATTGTTCCTTCTCATCCTCTCTATGCAATCTATGCTGCTACGAAAGCGTATGTAATCAAATTCTCAACTCTCTTCCTTAATATATTTTCTTGTTCTTGTTCTTGTTGTTCTTCTTTTAGGGAATAAAGAAAATGAAGAAGGTTTGAGGGAAGAAGTCCATCTCTTTCTCTATCTTCCTGAGGAAATAAGTGTTCACTGAGATGGGCCTCCCTCACTTACCCCATGTCTCACCAACTACCATGTCTTCTGCCAAAGTTCCTAATTAATTTTTGAGAAATTATTGACGTCTTCTCTGCTTTTTGAAATAAGAAAAAGTACAGGTACGGTTGAAAAGGTTTTGGCATGTGCTGTTGTCAGAAGTTTATTCAAAATCACTCCAAAATATGTTGTTAGTCCTTCTAAATCAAAGTTGATGATATGAAAATTGGCTTTAAAAGTGTAATTAAAGTCATGTTTCCGAGTCATTTTTAACATGACATGATTGATTTCAACAATTTCAAAAACACTCTCAAAACGTGGGAAATCCAACTCATTTTTCCATCCGTACACGGGTCCCCAACTTCCACCTCCAAGTTAAAAACCTCTACTTTTTCTGATTATGGGATAATCGAAAAGCTGAAAAGCAACCCTCCATTTGATCCTTGTTGAATGTTGATGATATGATATAATCATGTTGATCTTTCTAATTCTTTCTTTCTTTTCTCAGGTACGTTGACCAACTCTCAAGATCTCTACACGTGGAATACAAAGATTGGGGAATTGATGTTCAATGTCAGGTGCCGTTGTACGTCGCAACTGAAATGGCATCAAGAGTAGCGTCGGTAAGCCGAGCGTCGCTGTTCATTCCATCAGCCGATGACTATGTGAAAGCTGCAATTCGCCAAATTGGATACGAGCCACGGTGCACCCCTTACTGGGCGCACTCGCTCCAATGGTGTTTTGCTTCCCTGCTCCCGGAGGCGATGCTTGACGCCTGGCGGCTCTCCATTGGCTTGGAGAGGAGGAGGAAGGAATCCGTCACCATGAAACGGATTGAAGGTTGCAAACAAGAATGA

mRNA sequence

ATGAAGGAGACATTCTTTCTGCTACTTTCCACTAGTCCCATTTGGCTTCTCATCCTCTCCTTTCTCGGTTTCTTCACCATTCTTTGGCACTCCGCCACTGTCGCCCGATGGGTTTACCGAGCCTTTCTCAGACCCTCCAAGGATCTGAGATACTATGGCTCTTGGGGAATAGTCACTGGCGCCACTGACGGCATTGGAAAGTCCTTTGCCTATCAGCTGGCCAGAGCCGGACTCAACCTTGTACTGGTGAGCAGAAGCTCCATGAAGCTTAAGGCAGTTTCTAAGGATATTCAATCTGAATTTCCTGATACAAAGGTAAAGATAATTGAATTAGACTTCACAGAAGACGATATTAGCGGTGGTATTGCAGAGATTGAGGAGGTGATTGAAGATTTAGATGTGGGGATTTTGATAAACAACGTGGGGATTACATATCCGAACGCTAGCTTTTTCCATGAAGTGGACGAGAAAGTTTGGATGAATGTTTTCAAAGTGAATGTGAAGGGAACTACTTGGGTTACTAAAGTTGTTTTGCCAAAGATGATTAAGAAGAACAGAGGAGCTATTGTGAATATTGGCTCTGGAGCTGCTGTTATTGTTCCTTCTCATCCTCTCTATGCAATCTATGCTGCTACGAAAGCGTACGTTGACCAACTCTCAAGATCTCTACACGTGGAATACAAAGATTGGGGAATTGATGTTCAATGTCAGGTGCCGTTGTACGTCGCAACTGAAATGGCATCAAGAGTAGCGTCGGTAAGCCGAGCGTCGCTGTTCATTCCATCAGCCGATGACTATGTGAAAGCTGCAATTCGCCAAATTGGATACGAGCCACGGTGCACCCCTTACTGGGCGCACTCGCTCCAATGGTGTTTTGCTTCCCTGCTCCCGGAGGCGATGCTTGACGCCTGGCGGCTCTCCATTGGCTTGGAGAGGAGGAGGAAGGAATCCGTCACCATGAAACGGATTGAAGGTTGCAAACAAGAATGA

Coding sequence (CDS)

ATGAAGGAGACATTCTTTCTGCTACTTTCCACTAGTCCCATTTGGCTTCTCATCCTCTCCTTTCTCGGTTTCTTCACCATTCTTTGGCACTCCGCCACTGTCGCCCGATGGGTTTACCGAGCCTTTCTCAGACCCTCCAAGGATCTGAGATACTATGGCTCTTGGGGAATAGTCACTGGCGCCACTGACGGCATTGGAAAGTCCTTTGCCTATCAGCTGGCCAGAGCCGGACTCAACCTTGTACTGGTGAGCAGAAGCTCCATGAAGCTTAAGGCAGTTTCTAAGGATATTCAATCTGAATTTCCTGATACAAAGGTAAAGATAATTGAATTAGACTTCACAGAAGACGATATTAGCGGTGGTATTGCAGAGATTGAGGAGGTGATTGAAGATTTAGATGTGGGGATTTTGATAAACAACGTGGGGATTACATATCCGAACGCTAGCTTTTTCCATGAAGTGGACGAGAAAGTTTGGATGAATGTTTTCAAAGTGAATGTGAAGGGAACTACTTGGGTTACTAAAGTTGTTTTGCCAAAGATGATTAAGAAGAACAGAGGAGCTATTGTGAATATTGGCTCTGGAGCTGCTGTTATTGTTCCTTCTCATCCTCTCTATGCAATCTATGCTGCTACGAAAGCGTACGTTGACCAACTCTCAAGATCTCTACACGTGGAATACAAAGATTGGGGAATTGATGTTCAATGTCAGGTGCCGTTGTACGTCGCAACTGAAATGGCATCAAGAGTAGCGTCGGTAAGCCGAGCGTCGCTGTTCATTCCATCAGCCGATGACTATGTGAAAGCTGCAATTCGCCAAATTGGATACGAGCCACGGTGCACCCCTTACTGGGCGCACTCGCTCCAATGGTGTTTTGCTTCCCTGCTCCCGGAGGCGATGCTTGACGCCTGGCGGCTCTCCATTGGCTTGGAGAGGAGGAGGAAGGAATCCGTCACCATGAAACGGATTGAAGGTTGCAAACAAGAATGA
Homology
[Close]
BLAST of CSPI05G21810 vs. Swiss-Prot
Match: KCR2_ARATH (Very-long-chain 3-oxoacyl-CoA reductase-like protein At1g24470 OS=Arabidopsis thaliana GN=KCR2 PE=2 SV=1)

HSP 1 Score: 355.5 bits (911), Expect = 6.2e-97
Identity = 177/304 (58.22%), Postives = 226/304 (74.34%), Query Frame = 1 

Query: 11  TSPIWLLILSFLGF-FTILWHSATVARWVYRAFLRPSKDLRYYGSWGIVTGATDGIGKSF 70
           + P +L  + F+GF F +      + +W    FL   K L+ YGSW +VTGAT+GIG++F
Sbjct: 9   SQPWYLHFVCFIGFLFLLRVLFIPLLKWFTTRFLLTPKRLKRYGSWAMVTGATEGIGRAF 68

Query: 71  AYQLARAGLNLVLVSRSSMKLKAVSKDIQSEFPDTKVKIIELDFTEDDISGGIAEIEEVI 130
           A++LA+ GLNL+LVSR+  KL++VS D Q EFP  K+KII  DF+ +   GG   IEE I
Sbjct: 69  AHELAKHGLNLILVSRNLSKLESVSDDFQQEFPHIKIKIIPFDFSSE---GGYGAIEEGI 128

Query: 131 EDLDVGILINNVGITYPNASFFHEVDEKVWMNVFKVNVKGTTWVTKVVLPKMIKKNRGAI 190
           + L+VGILINNVGITYP A FFHEVD+  W  + +VN++ TTWVT+ ++  M+ + RGAI
Sbjct: 129 KGLEVGILINNVGITYPRAMFFHEVDQLTWTKILRVNLEATTWVTRSLIGPMLHRRRGAI 188

Query: 191 VNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHVEYKDWGIDVQCQVPLYVATEMASR 250
           VNI SGAAV+VPSHPLYAIYAATKAYVD LSRSLHVEYK +GIDVQCQVPLYV+T M S 
Sbjct: 189 VNISSGAAVVVPSHPLYAIYAATKAYVDALSRSLHVEYKQFGIDVQCQVPLYVSTRMVSE 248

Query: 251 VASVSRASLFIPSADDYVKAAIRQIGYEPRCTPYWAHSLQWCFASLLPEAMLDAWRLSIG 310
           VA++ + SLF+PS + Y KAA+ QIG   RC+P+WAHSLQW    L+P+ ++D WRLSIG
Sbjct: 249 VAAIDKPSLFVPSPEVYAKAAVAQIGIGSRCSPFWAHSLQWFLVGLVPDNLVDTWRLSIG 308

Query: 311 LERR 314
           L RR
Sbjct: 309 LRRR 309

[Close]
BLAST of CSPI05G21810 vs. Swiss-Prot
Match: KCR1_ARATH (Very-long-chain 3-oxoacyl-CoA reductase 1 OS=Arabidopsis thaliana GN=KCR1 PE=1 SV=1)

HSP 1 Score: 322.8 bits (826), Expect = 4.4e-87
Identity = 162/305 (53.11%), Postives = 220/305 (72.13%), Query Frame = 1 

Query: 11  TSPIWLLILSFLGFFTILWHSATVARWVYRAFLRPSKDLRYYGSWGIVTGATDGIGKSFA 70
           + P WLLIL  LG  +I     T+ R  Y  FLRPSK+LR YGSW I+TG TDGIGK+FA
Sbjct: 9   SQPTWLLILFVLGSISIFKFIFTLLRSFYIYFLRPSKNLRRYGSWAIITGPTDGIGKAFA 68

Query: 71  YQLARAGLNLVLVSRSSMKLKAVSKDIQSEFPDTKVKIIELDFTEDDISGGIAEIEEVIE 130
           +QLA+ GLNL+LV+R+  KLK VS  I+S++  T++  + +DF+  DI  G+  I+E IE
Sbjct: 69  FQLAQKGLNLILVARNPDKLKDVSDSIRSKYSQTQILTVVMDFS-GDIDEGVKRIKESIE 128

Query: 131 DLDVGILINNVGITYPNASFFHEVDEKVWMNVFKVNVKGTTWVTKVVLPKMIKKNRGAIV 190
            LDVGILINN G++YP A +FHEVDE++  N+ K+NV+GTT VT+ VLP M+K+ +GAI+
Sbjct: 129 GLDVGILINNAGMSYPYAKYFHEVDEELINNLIKINVEGTTKVTQAVLPNMLKRKKGAII 188

Query: 191 NIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHVEYKDWGIDVQCQVPLYVATEMASRV 250
           N+GSGAA ++PS+P Y++YA  K YVDQ ++ LHVEYK  GIDVQCQVPLYVAT+M    
Sbjct: 189 NMGSGAAALIPSYPFYSVYAGAKTYVDQFTKCLHVEYKKSGIDVQCQVPLYVATKM---- 248

Query: 251 ASVSRASLFIPSADDYVKAAIRQIGYEPRCTPYWAHSLQWCFASLLPEAMLDAWRLSIGL 310
             + RAS  + S + Y KAA+R +GYE +CTPYW H+L     S LPE++ +++ +   L
Sbjct: 249 TKIRRASFLVASPEGYAKAALRFVGYEAQCTPYWPHALMGAVVSALPESVFESFNIKRCL 308

Query: 311 ERRRK 316
           + R+K
Sbjct: 309 QIRKK 308

[Close]
BLAST of CSPI05G21810 vs. Swiss-Prot
Match: HSDL1_HUMAN (Inactive hydroxysteroid dehydrogenase-like protein 1 OS=Homo sapiens GN=HSDL1 PE=1 SV=3)

HSP 1 Score: 194.1 bits (492), Expect = 2.4e-48
Identity = 108/274 (39.42%), Postives = 159/274 (58.03%), Query Frame = 1 

Query: 49  LRYYGSWGIVTGATDGIGKSFAYQLARAGLNLVLVSRSSMKLKAVSKDIQSEFPDTKVKI 108
           ++ YG W +V+GATDGIGK++A +LA  GLN++L+SR+  KL+ V+KDI   +       
Sbjct: 63  IKQYGRWAVVSGATDGIGKAYAEELASRGLNIILISRNEEKLQVVAKDIADTYK------ 122

Query: 109 IELDFTEDDISGG---IAEIEEVIEDLDVGILINNVGITYPNASFFHEVDEKVWMNVFKV 168
           +E D    D S G      I E ++D DVGIL+NNVG+ YP   +F ++ E    ++  V
Sbjct: 123 VETDIIVADFSSGREIYLPIREALKDKDVGILVNNVGVFYPYPQYFTQLSEDKLWDIINV 182

Query: 169 NVKGTTWVTKVVLPKMIKKNRGAIVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHV 228
           N+   + +  VVLP M+++ +GAIV I SG+       P  A ++A+KAY+D  SR+L  
Sbjct: 183 NIAAASLMVHVVLPGMVERKKGAIVTISSGSC--CKPTPQLAAFSASKAYLDHFSRALQY 242

Query: 229 EYKDWGIDVQCQVPLYVATEMASRVASVSRASLFIPSADDYVKAAIRQIGYEPRCTPYWA 288
           EY   GI VQ  +P YVAT M +    + R S  +PS   Y   A+  +G   R T YW+
Sbjct: 243 EYASKGIFVQSLIPFYVATSMTAPSNFLHRCSWLVPSPKVYAHHAVSTLGISKRTTGYWS 302

Query: 289 HSLQWCFASLLPEAMLDAWRLSIGLERRRKESVT 320
           HS+Q+ FA  +PE  L  W  +I     RKE+++
Sbjct: 303 HSIQFLFAQYMPE-WLWVWGANILNRSLRKEALS 327

[Close]
BLAST of CSPI05G21810 vs. Swiss-Prot
Match: HSDL1_BOVIN (Inactive hydroxysteroid dehydrogenase-like protein 1 OS=Bos taurus GN=HSDL1 PE=2 SV=1)

HSP 1 Score: 193.4 bits (490), Expect = 4.0e-48
Identity = 112/291 (38.49%), Postives = 167/291 (57.39%), Query Frame = 1 

Query: 34  VARWVYRAFLRPSKDLRYYGSWGIVTGATDGIGKSFAYQLARAGLNLVLVSRSSMKLKAV 93
           + R V RA L     ++ YG W +V+GATDGIG+++A +LA  GLN+VL+SRS  KL+ V
Sbjct: 53  IPRLVSRADL-----IKQYGRWAVVSGATDGIGRAYAEELASRGLNIVLISRSQEKLQMV 112

Query: 94  SKDIQSEFPDTKVKIIELDFTEDDISGG---IAEIEEVIEDLDVGILINNVGITYPNASF 153
           +KDI   +       +E D    D S G      I E ++D D+GIL+NNVG+ YP   +
Sbjct: 113 AKDIADTYK------VETDIIVADFSSGREIYDMIREALQDRDIGILVNNVGVFYPYPQY 172

Query: 154 FHEVDEKVWMNVFKVNVKGTTWVTKVVLPKMIKKNRGAIVNIGSGAAVIVPSHPLYAIYA 213
           F +V E    ++  VN+   + +  +VLP M+++ +GAIV I SG+       P  A ++
Sbjct: 173 FTQVSEDTLWDIVNVNIAAASLMVHIVLPGMVERKKGAIVTISSGSCC--KPTPQLAAFS 232

Query: 214 ATKAYVDQLSRSLHVEYKDWGIDVQCQVPLYVATEMASRVASVSRASLFIPSADDYVKAA 273
           A+KAY+D  SR+L  EY   GI VQ  +P YVAT +A+  + + +    +PS   Y   A
Sbjct: 233 ASKAYLDHFSRALQYEYASKGIFVQSLIPFYVATNVATPGSFLHKCPWLVPSPKVYAHHA 292

Query: 274 IRQIGYEPRCTPYWAHSLQWCFASLLPEAMLDAWRLSIGLERRRKESVTMK 322
           +  +G   R T YW+HS+Q+ FA  +PE  L  W  +I     RKE+++ K
Sbjct: 293 VSTLGISKRTTGYWSHSIQFLFAQYMPE-WLWVWGANILNRSLRKEALSCK 329

[Close]
BLAST of CSPI05G21810 vs. Swiss-Prot
Match: HSDL1_PONAB (Inactive hydroxysteroid dehydrogenase-like protein 1 OS=Pongo abelii GN=HSDL1 PE=2 SV=1)

HSP 1 Score: 191.0 bits (484), Expect = 2.0e-47
Identity = 105/274 (38.32%), Postives = 159/274 (58.03%), Query Frame = 1 

Query: 49  LRYYGSWGIVTGATDGIGKSFAYQLARAGLNLVLVSRSSMKLKAVSKDIQSEFPDTKVKI 108
           ++ YG W +V+GATDGIG+++A +LA  GLN++L+SR+  KL+ V+KDI   +       
Sbjct: 63  IKQYGRWAVVSGATDGIGRAYAEELASRGLNIILISRNEEKLQVVAKDIADTYK------ 122

Query: 109 IELDFTEDDISGG---IAEIEEVIEDLDVGILINNVGITYPNASFFHEVDEKVWMNVFKV 168
           +E      D S G      I E ++D D+GIL+NNVG+ YP   +F ++ E    ++  V
Sbjct: 123 VETGIIVADFSSGREIYLPIREALKDKDIGILVNNVGVFYPYPQYFTQLSEDKLWDIINV 182

Query: 169 NVKGTTWVTKVVLPKMIKKNRGAIVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHV 228
           N+   + +  VVLP M+++ +GAIV I SG+       P  A ++A+KAY+D  SR+L  
Sbjct: 183 NIAAASLMVHVVLPGMVERKKGAIVTISSGSC--CKPTPQLAAFSASKAYLDHFSRALQY 242

Query: 229 EYKDWGIDVQCQVPLYVATEMASRVASVSRASLFIPSADDYVKAAIRQIGYEPRCTPYWA 288
           EY   GI VQ  +P YVAT M +  + + R S  +PS   Y   A+  +G   R T YW+
Sbjct: 243 EYASKGIFVQSLIPFYVATSMTAPSSFLHRCSWLVPSPKVYAHHAVSTLGISKRTTGYWS 302

Query: 289 HSLQWCFASLLPEAMLDAWRLSIGLERRRKESVT 320
           HS+Q+ FA  +PE  L  W  +I     RKE+++
Sbjct: 303 HSIQFLFAQYMPE-WLWVWGANILNRSLRKEALS 327

[Close]
BLAST of CSPI05G21810 vs. TrEMBL
Match: A0A0A0KS33_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G591790 PE=3 SV=1)

HSP 1 Score: 648.3 bits (1671), Expect = 5.1e-183
Identity = 327/329 (99.39%), Postives = 328/329 (99.70%), Query Frame = 1 

Query: 1   MKETFFLLLSTSPIWLLILSFLGFFTILWHSATVARWVYRAFLRPSKDLRYYGSWGIVTG 60
           MKETFFLLLSTSPIWLLILSFLGFFTILWHSATVARWVYRAFLRPSKDLRYYGSWGIVTG
Sbjct: 4   MKETFFLLLSTSPIWLLILSFLGFFTILWHSATVARWVYRAFLRPSKDLRYYGSWGIVTG 63

Query: 61  ATDGIGKSFAYQLARAGLNLVLVSRSSMKLKAVSKDIQSEFPDTKVKIIELDFTEDDISG 120
           ATDGIGKSFAYQLARAGLNLVLVSRSSMKLKAVSKDIQSEFPDTKVKIIELDFTEDDISG
Sbjct: 64  ATDGIGKSFAYQLARAGLNLVLVSRSSMKLKAVSKDIQSEFPDTKVKIIELDFTEDDISG 123

Query: 121 GIAEIEEVIEDLDVGILINNVGITYPNASFFHEVDEKVWMNVFKVNVKGTTWVTKVVLPK 180
           GIAEIEEVIEDLDVGILINNVGITYPNASFFHEVDEKVWMNVFKVNVKGTTWVTK VLPK
Sbjct: 124 GIAEIEEVIEDLDVGILINNVGITYPNASFFHEVDEKVWMNVFKVNVKGTTWVTKAVLPK 183

Query: 181 MIKKNRGAIVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHVEYKDWGIDVQCQVPL 240
           MIKKNRGAIVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHVEYKDWGIDVQCQVPL
Sbjct: 184 MIKKNRGAIVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHVEYKDWGIDVQCQVPL 243

Query: 241 YVATEMASRVASVSRASLFIPSADDYVKAAIRQIGYEPRCTPYWAHSLQWCFASLLPEAM 300
           YVATEMASRVASVS+ASLFIPSADDYVKAAIRQIGYEPRCTPYWAHSLQWCFASLLPEAM
Sbjct: 244 YVATEMASRVASVSQASLFIPSADDYVKAAIRQIGYEPRCTPYWAHSLQWCFASLLPEAM 303

Query: 301 LDAWRLSIGLERRRKESVTMKRIEGCKQE 330
           LDAWRLSIGLERRRKESVTMKRIEGCKQE
Sbjct: 304 LDAWRLSIGLERRRKESVTMKRIEGCKQE 332

[Close]
BLAST of CSPI05G21810 vs. TrEMBL
Match: M5XGG4_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa015113mg PE=3 SV=1)

HSP 1 Score: 418.3 bits (1074), Expect = 8.6e-114
Identity = 203/307 (66.12%), Postives = 251/307 (81.76%), Query Frame = 1 

Query: 9   LSTSPIWLLILSFLGFFTILWHSATVARWVYRAFLRPSKDLRYYGSWGIVTGATDGIGKS 68
           L+  PIWLL+LS LGFF  L  + ++A WV+   LRP K+L+ YGSW +VTGATDGIGK+
Sbjct: 11  LTNQPIWLLLLSSLGFFIFLKQATSLANWVFIVLLRPPKNLKRYGSWAMVTGATDGIGKA 70

Query: 69  FAYQLARAGLNLVLVSRSSMKLKAVSKDIQSEFPDTKVKIIELDFTEDDISGGIAEIEEV 128
           FAYQLA+ GLNL+LVSR+S KLK+VSK+IQ+ +P T++K I  DF+   + G    +EE 
Sbjct: 71  FAYQLAQQGLNLILVSRNSAKLKSVSKEIQANYPTTQIKTIAFDFSVGSLGGMNELLEEE 130

Query: 129 IEDLDVGILINNVGITYPNASFFHEVDEKVWMNVFKVNVKGTTWVTKVVLPKMIKKNRGA 188
           I  LDVG+LINNVG+TYP+A F HEVDE+VW NV KVNV+GTT VT  VL  M+++ RGA
Sbjct: 131 IRGLDVGVLINNVGVTYPSARFLHEVDEQVWTNVVKVNVEGTTRVTMAVLRGMVERKRGA 190

Query: 189 IVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHVEYKDWGIDVQCQVPLYVATEMAS 248
           +VNIGSGA ++VPSHPL+ +YAATKAYVDQLSRSLHVEYK +GIDVQ QVPLYVAT++AS
Sbjct: 191 VVNIGSGAGIVVPSHPLFTVYAATKAYVDQLSRSLHVEYKRYGIDVQSQVPLYVATKLAS 250

Query: 249 RVASVSRASLFIPSADDYVKAAIRQIGYEPRCTPYWAHSLQWCFASLLPEAMLDAWRLSI 308
           +VAS+ R+SLFIP+   Y KAAIR+IGYE RCTP+WAHSLQWC  SL+PE++LDAWRLSI
Sbjct: 251 KVASIERSSLFIPTPHAYAKAAIRRIGYEARCTPFWAHSLQWCLGSLVPESLLDAWRLSI 310

Query: 309 GLERRRK 316
           GL+RR K
Sbjct: 311 GLKRRAK 317

[Close]
BLAST of CSPI05G21810 vs. TrEMBL
Match: B9RMQ7_RICCO (Steroid dehydrogenase, putative OS=Ricinus communis GN=RCOM_1082610 PE=3 SV=1)

HSP 1 Score: 415.2 bits (1066), Expect = 7.3e-113
Identity = 205/306 (66.99%), Postives = 248/306 (81.05%), Query Frame = 1 

Query: 9   LSTSPIWLLILSFLGFFTILWHSATVARWVYRAFLRPSKDLRY-YGSWGIVTGATDGIGK 68
           L+T P+WLLI+SFLGF ++  +S T+  WVY+  LR  K+L+  YGSW ++TGATDGIGK
Sbjct: 8   LTTQPLWLLIVSFLGFLSLFNYSFTLLNWVYKTVLRQPKNLKENYGSWALITGATDGIGK 67

Query: 69  SFAYQLARAGLNLVLVSRSSMKLKAVSKDIQSEFPDTKVKIIELDFTEDDISGGIAEIEE 128
           +FA+QLA+ GLNL+LVSR+  KLK VS +IQ EFP TK+KI+ LDF+ +D SG +  IEE
Sbjct: 68  AFAFQLAKQGLNLILVSRNLNKLKTVSSEIQEEFPSTKIKIMNLDFSSEDSSGLVHVIEE 127

Query: 129 VIEDLDVGILINNVGITYPNASFFHEVDEKVWMNVFKVNVKGTTWVTKVVLPKMIKKNRG 188
            I+ +DVG+LINNVGITYP A FF EVDE  WM+V KVNVKGTT VT+ VL  MI++ RG
Sbjct: 128 AIKGVDVGVLINNVGITYPQARFFDEVDESTWMSVVKVNVKGTTRVTEAVLSGMIERRRG 187

Query: 189 AIVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHVEYKDWGIDVQCQVPLYVATEMA 248
           AIVNIGSGA+++VPSHPLY IYAATKAY+DQLSR L+VEY+  GIDVQCQVPLYVAT M 
Sbjct: 188 AIVNIGSGASIVVPSHPLYTIYAATKAYIDQLSRCLYVEYRSCGIDVQCQVPLYVATNMT 247

Query: 249 SRVASVSRASLFIPSADDYVKAAIRQIGYEPRCTPYWAHSLQWCFASLLPEAMLDAWRLS 308
           SRVA + ++SLFIPS   Y +AAIR IGYE RCTPYWAHSLQW F  LLPEA+LD+WRLS
Sbjct: 248 SRVALIEKSSLFIPSPQAYAEAAIRCIGYEARCTPYWAHSLQWFFVRLLPEAVLDSWRLS 307

Query: 309 IGLERR 314
           IG+ RR
Sbjct: 308 IGIHRR 313

[Close]
BLAST of CSPI05G21810 vs. TrEMBL
Match: W9S1A9_9ROSA (Inactive hydroxysteroid dehydrogenase-like protein 1 OS=Morus notabilis GN=L484_027623 PE=3 SV=1)

HSP 1 Score: 414.1 bits (1063), Expect = 1.6e-112
Identity = 206/310 (66.45%), Postives = 253/310 (81.61%), Query Frame = 1 

Query: 7   LLLSTSPIWLL--ILSFLGFFTILWHSATVARWVYRAFLRPSKDL-RYYGSWGIVTGATD 66
           L ++  PI+LL  I++ LG FTIL  S ++ +WV+  F+RPSK+L + YGSW +VTGATD
Sbjct: 10  LYVTNQPIFLLLIIITSLGLFTILGRSISLLKWVFITFIRPSKNLIKIYGSWAMVTGATD 69

Query: 67  GIGKSFAYQLARAGLNLVLVSRSSMKLKAVSKDIQSEFPDTKVKIIELDFTEDDISGGIA 126
           GIGK+FAYQLA+ GLNL+LV R+S KLK VSK+I SEFP T++K +  DF+  D+S GI 
Sbjct: 70  GIGKAFAYQLAKQGLNLILVGRNSNKLKTVSKEILSEFPTTRIKTVIFDFS-GDVSAGIK 129

Query: 127 EIEEVIEDLDVGILINNVGITYPNASFFHEVDEKVWMNVFKVNVKGTTWVTKVVLPKMIK 186
            +EE IE LDVG+LINN G+TYP A +FHEVDEKVWMN+ KVN++GTT VT+ VLP M++
Sbjct: 130 AVEEAIEGLDVGVLINNAGVTYPAARYFHEVDEKVWMNIVKVNLEGTTRVTRAVLPGMLQ 189

Query: 187 KNRGAIVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHVEYKDWGIDVQCQVPLYVA 246
           + RGAIVNIGSGA+++VPSHPLY IYAATKAY+DQ SRSL+VEY   GIDVQCQVPLYV 
Sbjct: 190 RKRGAIVNIGSGASIVVPSHPLYTIYAATKAYIDQFSRSLYVEYNRSGIDVQCQVPLYVV 249

Query: 247 TEMASRVASVSRASLFIPSADDYVKAAIRQIGYEPRCTPYWAHSLQWCFASLLPEAMLDA 306
           T+M S VAS+ + SLFIPSA+DY KAAIR+IGYE RCTPYWAHSLQW FAS L E +LDA
Sbjct: 250 TKMVSEVASIEKTSLFIPSAEDYAKAAIRRIGYEARCTPYWAHSLQWFFASFLSEDILDA 309

Query: 307 WRLSIGLERR 314
           WRL+IG+ RR
Sbjct: 310 WRLAIGIRRR 318

[Close]
BLAST of CSPI05G21810 vs. TrEMBL
Match: A0A068UXM9_COFCA (Uncharacterized protein OS=Coffea canephora GN=GSCOC_T00038162001 PE=4 SV=1)

HSP 1 Score: 414.1 bits (1063), Expect = 1.6e-112
Identity = 198/306 (64.71%), Postives = 243/306 (79.41%), Query Frame = 1 

Query: 9   LSTSPIWLLILSFLGFFTILWHSATVARWVYRAFLRPSKDLRYYGSWGIVTGATDGIGKS 68
           L T P W+L L  +GF ++  +   V +W    F RP K L +YGSW ++TG+TDGIGK+
Sbjct: 9   LKTQPFWILCLFSVGFLSLSRNCMLVIKWGLTTFFRPPKKLTFYGSWALITGSTDGIGKA 68

Query: 69  FAYQLARAGLNLVLVSRSSMKLKAVSKDIQSEFPDTKVKIIELDFTEDDISGGIA-EIEE 128
           FA+ LA+ GLNLVLVSR+  KL+ VS +IQ+ FP T++K I LDF+ DD S  +  E+E+
Sbjct: 69  FAFDLAKKGLNLVLVSRNLEKLEQVSTEIQAGFPTTRIKTIALDFSRDDASSSVLQEVEK 128

Query: 129 VIEDLDVGILINNVGITYPNASFFHEVDEKVWMNVFKVNVKGTTWVTKVVLPKMIKKNRG 188
            IE LD+G+LINNVG+TYP A FFHEVDE+VWMNV +VN+KGTT VTK VLP M+KK RG
Sbjct: 129 AIEGLDIGVLINNVGVTYPGARFFHEVDEEVWMNVVRVNLKGTTLVTKAVLPGMLKKKRG 188

Query: 189 AIVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHVEYKDWGIDVQCQVPLYVATEMA 248
           AIVN+GSGAA+IVPSHPLYAIYAATKAY+DQ SRSLHVEYK WG+DVQCQVPLYV T+MA
Sbjct: 189 AIVNLGSGAAIIVPSHPLYAIYAATKAYIDQFSRSLHVEYKGWGVDVQCQVPLYVWTKMA 248

Query: 249 SRVASVSRASLFIPSADDYVKAAIRQIGYEPRCTPYWAHSLQWCFASLLPEAMLDAWRLS 308
           SRVA + ++S F+P+A+DYV AA+ +IGYE RCTP+W HSLQW  AS LPEA+LD WRLS
Sbjct: 249 SRVAEIEKSSFFVPTAEDYVSAAVGRIGYEARCTPWWTHSLQWSLASFLPEAVLDRWRLS 308

Query: 309 IGLERR 314
           IG  RR
Sbjct: 309 IGTRRR 314

[Close]
BLAST of CSPI05G21810 vs. TAIR10
Match: AT1G24470.1 (AT1G24470.1 beta-ketoacyl reductase 2)

HSP 1 Score: 355.5 bits (911), Expect = 3.5e-98
Identity = 177/304 (58.22%), Postives = 226/304 (74.34%), Query Frame = 1 

Query: 11  TSPIWLLILSFLGF-FTILWHSATVARWVYRAFLRPSKDLRYYGSWGIVTGATDGIGKSF 70
           + P +L  + F+GF F +      + +W    FL   K L+ YGSW +VTGAT+GIG++F
Sbjct: 9   SQPWYLHFVCFIGFLFLLRVLFIPLLKWFTTRFLLTPKRLKRYGSWAMVTGATEGIGRAF 68

Query: 71  AYQLARAGLNLVLVSRSSMKLKAVSKDIQSEFPDTKVKIIELDFTEDDISGGIAEIEEVI 130
           A++LA+ GLNL+LVSR+  KL++VS D Q EFP  K+KII  DF+ +   GG   IEE I
Sbjct: 69  AHELAKHGLNLILVSRNLSKLESVSDDFQQEFPHIKIKIIPFDFSSE---GGYGAIEEGI 128

Query: 131 EDLDVGILINNVGITYPNASFFHEVDEKVWMNVFKVNVKGTTWVTKVVLPKMIKKNRGAI 190
           + L+VGILINNVGITYP A FFHEVD+  W  + +VN++ TTWVT+ ++  M+ + RGAI
Sbjct: 129 KGLEVGILINNVGITYPRAMFFHEVDQLTWTKILRVNLEATTWVTRSLIGPMLHRRRGAI 188

Query: 191 VNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHVEYKDWGIDVQCQVPLYVATEMASR 250
           VNI SGAAV+VPSHPLYAIYAATKAYVD LSRSLHVEYK +GIDVQCQVPLYV+T M S 
Sbjct: 189 VNISSGAAVVVPSHPLYAIYAATKAYVDALSRSLHVEYKQFGIDVQCQVPLYVSTRMVSE 248

Query: 251 VASVSRASLFIPSADDYVKAAIRQIGYEPRCTPYWAHSLQWCFASLLPEAMLDAWRLSIG 310
           VA++ + SLF+PS + Y KAA+ QIG   RC+P+WAHSLQW    L+P+ ++D WRLSIG
Sbjct: 249 VAAIDKPSLFVPSPEVYAKAAVAQIGIGSRCSPFWAHSLQWFLVGLVPDNLVDTWRLSIG 308

Query: 311 LERR 314
           L RR
Sbjct: 309 LRRR 309

[Close]
BLAST of CSPI05G21810 vs. TAIR10
Match: AT1G67730.1 (AT1G67730.1 beta-ketoacyl reductase 1)

HSP 1 Score: 322.8 bits (826), Expect = 2.5e-88
Identity = 162/305 (53.11%), Postives = 220/305 (72.13%), Query Frame = 1 

Query: 11  TSPIWLLILSFLGFFTILWHSATVARWVYRAFLRPSKDLRYYGSWGIVTGATDGIGKSFA 70
           + P WLLIL  LG  +I     T+ R  Y  FLRPSK+LR YGSW I+TG TDGIGK+FA
Sbjct: 9   SQPTWLLILFVLGSISIFKFIFTLLRSFYIYFLRPSKNLRRYGSWAIITGPTDGIGKAFA 68

Query: 71  YQLARAGLNLVLVSRSSMKLKAVSKDIQSEFPDTKVKIIELDFTEDDISGGIAEIEEVIE 130
           +QLA+ GLNL+LV+R+  KLK VS  I+S++  T++  + +DF+  DI  G+  I+E IE
Sbjct: 69  FQLAQKGLNLILVARNPDKLKDVSDSIRSKYSQTQILTVVMDFS-GDIDEGVKRIKESIE 128

Query: 131 DLDVGILINNVGITYPNASFFHEVDEKVWMNVFKVNVKGTTWVTKVVLPKMIKKNRGAIV 190
            LDVGILINN G++YP A +FHEVDE++  N+ K+NV+GTT VT+ VLP M+K+ +GAI+
Sbjct: 129 GLDVGILINNAGMSYPYAKYFHEVDEELINNLIKINVEGTTKVTQAVLPNMLKRKKGAII 188

Query: 191 NIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHVEYKDWGIDVQCQVPLYVATEMASRV 250
           N+GSGAA ++PS+P Y++YA  K YVDQ ++ LHVEYK  GIDVQCQVPLYVAT+M    
Sbjct: 189 NMGSGAAALIPSYPFYSVYAGAKTYVDQFTKCLHVEYKKSGIDVQCQVPLYVATKM---- 248

Query: 251 ASVSRASLFIPSADDYVKAAIRQIGYEPRCTPYWAHSLQWCFASLLPEAMLDAWRLSIGL 310
             + RAS  + S + Y KAA+R +GYE +CTPYW H+L     S LPE++ +++ +   L
Sbjct: 249 TKIRRASFLVASPEGYAKAALRFVGYEAQCTPYWPHALMGAVVSALPESVFESFNIKRCL 308

Query: 311 ERRRK 316
           + R+K
Sbjct: 309 QIRKK 308

[Close]
BLAST of CSPI05G21810 vs. TAIR10
Match: AT5G10050.1 (AT5G10050.1 NAD(P)-binding Rossmann-fold superfamily protein)

HSP 1 Score: 78.6 bits (192), Expect = 8.2e-15
Identity = 64/202 (31.68%), Postives = 107/202 (52.97%), Query Frame = 1 

Query: 57  IVTGATDG-IGKSFAYQLARAGLNLVLVSRSSMKLKAVSKDIQSEFPDTKVKIIELDFTE 116
           ++TG + G IG + A +    G  +V  SRS    ++   D++    D+++ + ELD   
Sbjct: 11  LITGCSQGGIGHALAREFTEKGCRVVATSRS----RSTMTDLEQ---DSRLFVKELDVQS 70

Query: 117 D-DISGGIAEIEEVIEDLDVGILINNVGITYPNASFFHEVDEKVWMNVFKVNVKGTTWVT 176
           D ++S  ++E+ +    +DV  L+NN G+         E       N F  NV G+  +T
Sbjct: 71  DQNVSKVLSEVIDKFGKIDV--LVNNAGVQCVGP--LAETPISAMENTFNTNVFGSMRMT 130

Query: 177 KVVLPKMIKKNRGAIVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHVEYKDWGIDV 236
           + V+P M+ K +G IVN+GS   V+ P  P   +Y ATKA +  L+ +L +E + +GIDV
Sbjct: 131 QAVVPHMVSKKKGKIVNVGS-ITVMAPG-PWAGVYTATKAAIHALTDTLRLELRPFGIDV 190

Query: 237 QCQVPLYVATEMA-SRVASVSR 256
              VP  + T +A S VA+ ++
Sbjct: 191 INVVPGGIRTNIANSAVATFNK 199

[Close]
BLAST of CSPI05G21810 vs. TAIR10
Match: AT3G03330.1 (AT3G03330.1 NAD(P)-binding Rossmann-fold superfamily protein)

HSP 1 Score: 77.0 bits (188), Expect = 2.4e-14
Identity = 72/236 (30.51%), Postives = 108/236 (45.76%), Query Frame = 1 

Query: 58  VTGATDGIGKSFAYQLARAGLNLVLVSRSSMKLKAVSKDIQSEFPDTKVKIIELDFT--E 117
           +TGA+ GIG+  A Q A     L+L +R+  +L  V  +++ +F    VK++ LD    E
Sbjct: 48  ITGASRGIGEILAKQFASLDAKLILSARNKAELDRVKSELKGKFAPEDVKVLPLDLASGE 107

Query: 118 DDISGGIAEIEEVIEDLDVGILINNVGITYPNASFFHEVDEKVWMNVFKVNVKGTTWVTK 177
           + +   + +   +     V  L++N     P +    +  E+     F VNV GT  +TK
Sbjct: 108 ESLKHVVEQAVSLFPGAGVDYLVHNAAFERPKSKA-SDASEETLKTTFDVNVFGTISLTK 167

Query: 178 VVLPKMIKKNRGAIVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHVEYKDWGIDVQ 237
           +V P M+K+  G  V I S AA  VPS P  AIY+A+K  +     SL  E+   GI V 
Sbjct: 168 LVAPHMLKQGGGHFVVI-SSAAGKVPS-PGQAIYSASKHALHGYFHSLRSEFCQKGIKVT 227

Query: 238 CQVPLYVAT-------------EMASRVASVSRASLFIPSADDYVKAAIRQIGYEP 279
              P  + T                 RV+S   A L I +A   +K A   I Y+P
Sbjct: 228 VVCPGPIETWNGTGTSTSEDSKSPEKRVSSERCAELTIIAASHNLKEA--WISYQP 278

[Close]
BLAST of CSPI05G21810 vs. TAIR10
Match: AT5G65205.1 (AT5G65205.1 NAD(P)-binding Rossmann-fold superfamily protein)

HSP 1 Score: 73.9 bits (180), Expect = 2.0e-13
Identity = 72/257 (28.02%), Postives = 118/257 (45.91%), Query Frame = 1 

Query: 57  IVTGATDG-IGKSFAYQLARAGLNLVLVSRSSMKLKAVSKDIQSEFPDTKVKIIELDF-T 116
           ++TG + G IG + A + +  G  +V  SRS   +  + KD       +K  + ELD  +
Sbjct: 12  LITGCSQGGIGHALAREFSANGCRVVATSRSQKTMTELEKD-------SKFFVQELDVQS 71

Query: 117 EDDISGGIAEIEEVIEDLDVGILINNVGITYPNASFFHEVDEKVWMNVFKVNVKGTTWVT 176
           E  +S  ++++ +    +DV  L+NN G+         E+        F  NV G+  +T
Sbjct: 72  EQSVSKVVSKVIDKFGQIDV--LVNNAGVQCIGP--LAEIPISAMDYTFNTNVLGSMRMT 131

Query: 177 KVVLPKMIKKNRGAIVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHVEYKDWGIDV 236
           + V+P M  K +G IVNIGS  +++ P  P   +Y A+KA +  L+ +L +E K +GIDV
Sbjct: 132 QAVVPHMASKKKGKIVNIGS-ISIMAPG-PWAGVYTASKAALHALTDTLRLELKPFGIDV 191

Query: 237 QCQVPLYVATEMA----SRVASVSRASLFIPSADDYVKAAIRQIGYEPRCTPYWAHSLQW 296
              VP  + + +A    S   ++    L+ P  D   + A      +P  T  +A     
Sbjct: 192 INIVPGGIQSNIANSGISSFNNLPELKLYKPFEDAIRERAFLSQNIKPIPTETFAKETVS 251

Query: 297 CFASLLPEAMLDAWRLS 308
                 P A     RLS
Sbjct: 252 VVLKKNPPAWYSTGRLS 255

[Close]
BLAST of CSPI05G21810 vs. NCBI nr
Match: gi|449435019|ref|XP_004135293.1| (PREDICTED: very-long-chain 3-oxoacyl-CoA reductase-like protein At1g24470 [Cucumis sativus])

HSP 1 Score: 648.3 bits (1671), Expect = 7.3e-183
Identity = 327/329 (99.39%), Postives = 328/329 (99.70%), Query Frame = 1 

Query: 1   MKETFFLLLSTSPIWLLILSFLGFFTILWHSATVARWVYRAFLRPSKDLRYYGSWGIVTG 60
           MKETFFLLLSTSPIWLLILSFLGFFTILWHSATVARWVYRAFLRPSKDLRYYGSWGIVTG
Sbjct: 4   MKETFFLLLSTSPIWLLILSFLGFFTILWHSATVARWVYRAFLRPSKDLRYYGSWGIVTG 63

Query: 61  ATDGIGKSFAYQLARAGLNLVLVSRSSMKLKAVSKDIQSEFPDTKVKIIELDFTEDDISG 120
           ATDGIGKSFAYQLARAGLNLVLVSRSSMKLKAVSKDIQSEFPDTKVKIIELDFTEDDISG
Sbjct: 64  ATDGIGKSFAYQLARAGLNLVLVSRSSMKLKAVSKDIQSEFPDTKVKIIELDFTEDDISG 123

Query: 121 GIAEIEEVIEDLDVGILINNVGITYPNASFFHEVDEKVWMNVFKVNVKGTTWVTKVVLPK 180
           GIAEIEEVIEDLDVGILINNVGITYPNASFFHEVDEKVWMNVFKVNVKGTTWVTK VLPK
Sbjct: 124 GIAEIEEVIEDLDVGILINNVGITYPNASFFHEVDEKVWMNVFKVNVKGTTWVTKAVLPK 183

Query: 181 MIKKNRGAIVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHVEYKDWGIDVQCQVPL 240
           MIKKNRGAIVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHVEYKDWGIDVQCQVPL
Sbjct: 184 MIKKNRGAIVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHVEYKDWGIDVQCQVPL 243

Query: 241 YVATEMASRVASVSRASLFIPSADDYVKAAIRQIGYEPRCTPYWAHSLQWCFASLLPEAM 300
           YVATEMASRVASVS+ASLFIPSADDYVKAAIRQIGYEPRCTPYWAHSLQWCFASLLPEAM
Sbjct: 244 YVATEMASRVASVSQASLFIPSADDYVKAAIRQIGYEPRCTPYWAHSLQWCFASLLPEAM 303

Query: 301 LDAWRLSIGLERRRKESVTMKRIEGCKQE 330
           LDAWRLSIGLERRRKESVTMKRIEGCKQE
Sbjct: 304 LDAWRLSIGLERRRKESVTMKRIEGCKQE 332

[Close]
BLAST of CSPI05G21810 vs. NCBI nr
Match: gi|659090591|ref|XP_008446096.1| (PREDICTED: very-long-chain 3-oxoacyl-CoA reductase-like protein At1g24470 [Cucumis melo])

HSP 1 Score: 611.7 bits (1576), Expect = 7.6e-172
Identity = 308/329 (93.62%), Postives = 318/329 (96.66%), Query Frame = 1 

Query: 1   MKETFFLLLSTSPIWLLILSFLGFFTILWHSATVARWVYRAFLRPSKDLRYYGSWGIVTG 60
           MKETFFLLLST+PIWLLILS LGFFTIL HSATVARWV+RAFLRPSKDLRYYGSWGIVTG
Sbjct: 4   MKETFFLLLSTNPIWLLILSLLGFFTILRHSATVARWVFRAFLRPSKDLRYYGSWGIVTG 63

Query: 61  ATDGIGKSFAYQLARAGLNLVLVSRSSMKLKAVSKDIQSEFPDTKVKIIELDFTEDDISG 120
           ATDGIGKSFAYQLARAGLNL+LVSRSS KLKAVS+DIQSEFPDTKVKIIELDFTEDDISG
Sbjct: 64  ATDGIGKSFAYQLARAGLNLILVSRSSTKLKAVSEDIQSEFPDTKVKIIELDFTEDDISG 123

Query: 121 GIAEIEEVIEDLDVGILINNVGITYPNASFFHEVDEKVWMNVFKVNVKGTTWVTKVVLPK 180
           GIAEIEEVIEDLDVGILINNVGITYPNASFFHEVDEKVWMN+FKVNVKGTTWVTK VLPK
Sbjct: 124 GIAEIEEVIEDLDVGILINNVGITYPNASFFHEVDEKVWMNLFKVNVKGTTWVTKAVLPK 183

Query: 181 MIKKNRGAIVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHVEYKDWGIDVQCQVPL 240
           MIKKNRGAIVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSL+VEYKDWGIDVQCQVPL
Sbjct: 184 MIKKNRGAIVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLYVEYKDWGIDVQCQVPL 243

Query: 241 YVATEMASRVASVSRASLFIPSADDYVKAAIRQIGYEPRCTPYWAHSLQWCFASLLPEAM 300
           YVATEMASRVASVSRASLF+PSADDYVKAAIRQIGYEPRCTPYWAHSLQWCFA LLPEA+
Sbjct: 244 YVATEMASRVASVSRASLFVPSADDYVKAAIRQIGYEPRCTPYWAHSLQWCFARLLPEAV 303

Query: 301 LDAWRLSIGLERRRKESVTMKRIEGCKQE 330
           LDAWRLSIGL+RR   SV M+  EGCKQE
Sbjct: 304 LDAWRLSIGLQRRSNGSVAMEGTEGCKQE 332

[Close]
BLAST of CSPI05G21810 vs. NCBI nr
Match: gi|1009153207|ref|XP_015894513.1| (PREDICTED: very-long-chain 3-oxoacyl-CoA reductase-like protein At1g24470 [Ziziphus jujuba])

HSP 1 Score: 438.7 bits (1127), Expect = 8.8e-120
Identity = 207/306 (67.65%), Postives = 260/306 (84.97%), Query Frame = 1 

Query: 9   LSTSPIWLLILSFLGFFTILWHSATVARWVYRAFLRPSKDLRYYGSWGIVTGATDGIGKS 68
           L T PIW L++S LGFFTIL  SAT   WV+  F+R  KDL+ YGSW +VTG+TDGIGK+
Sbjct: 10  LRTQPIWFLLISSLGFFTILKKSATFLNWVFITFIRDPKDLKRYGSWAMVTGSTDGIGKA 69

Query: 69  FAYQLARAGLNLVLVSRSSMKLKAVSKDIQSEFPDTKVKIIELDFTEDDISGGIAEIEEV 128
           FAYQLA+ GLNL+LVSR+S KLK+VSK+ Q+EFPDT++K I  DF+ D I  G+ EIEE 
Sbjct: 70  FAYQLAKKGLNLILVSRNSSKLKSVSKEFQAEFPDTQIKTIAFDFSSD-ILEGVKEIEEG 129

Query: 129 IEDLDVGILINNVGITYPNASFFHEVDEKVWMNVFKVNVKGTTWVTKVVLPKMIKKNRGA 188
           I+ L++GILINNVG++YP+A++FHEVDE+VWM++ +VN++GTTWV++ VLP M+++ RGA
Sbjct: 130 IKGLEIGILINNVGVSYPSAAYFHEVDEQVWMDIVRVNLEGTTWVSRAVLPGMLQRKRGA 189

Query: 189 IVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHVEYKDWGIDVQCQVPLYVATEMAS 248
           IVNIGSGAA++VPSHPLY  YAATK+Y+DQLSR LHVEYK  GIDVQCQVPLYVAT+MAS
Sbjct: 190 IVNIGSGAAIVVPSHPLYTTYAATKSYIDQLSRCLHVEYKHCGIDVQCQVPLYVATKMAS 249

Query: 249 RVASVSRASLFIPSADDYVKAAIRQIGYEPRCTPYWAHSLQWCFASLLPEAMLDAWRLSI 308
            +AS+ R+SLF+PS DDY +AAIR IGYE RCTPYW+H +QWCFA LLPEA+LD+WRLSI
Sbjct: 250 DIASIKRSSLFVPSPDDYAEAAIRHIGYEARCTPYWSHWVQWCFAGLLPEAILDSWRLSI 309

Query: 309 GLERRR 315
           G+ RR+
Sbjct: 310 GINRRQ 314

[Close]
BLAST of CSPI05G21810 vs. NCBI nr
Match: gi|470148953|ref|XP_004310011.1| (PREDICTED: very-long-chain 3-oxoacyl-CoA reductase-like protein At1g24470 [Fragaria vesca subsp. vesca])

HSP 1 Score: 430.6 bits (1106), Expect = 2.4e-117
Identity = 210/309 (67.96%), Postives = 263/309 (85.11%), Query Frame = 1 

Query: 8   LLSTSPIWLLILSFLGFFTILWHSATVARWVYRAFLRPSKDL-RYYGSWGIVTGATDGIG 67
           L +  P+WLL+LS LGFF  L HS ++ARW++  FLRP+KDL R YGSWG++TGATDGIG
Sbjct: 15  LTNQQPVWLLLLSSLGFFIFLKHSISLARWLFITFLRPAKDLERSYGSWGLITGATDGIG 74

Query: 68  KSFAYQLARAGLNLVLVSRSSMKLKAVSKDIQSEFPDTKVKIIELDFTEDDISGGIAEIE 127
           K+FAYQLA+ GLNLVLVSRSS KL++VSK+IQS FPD K+K +  DF+ D+  G +  +E
Sbjct: 75  KAFAYQLAKKGLNLVLVSRSSNKLESVSKEIQSHFPDVKIKTVAFDFSSDE--GLVKLVE 134

Query: 128 EVIEDLDVGILINNVGITYPNASFFHEVDEKVWMNVFKVNVKGTTWVTKVVLPKMIKKNR 187
           E I+ LDVGILINNVG+TYP AS+FHEVDEKVWMN+ +VN++GTT VT  V+P M+++ R
Sbjct: 135 EKIKGLDVGILINNVGVTYPAASYFHEVDEKVWMNIIRVNLEGTTRVTMAVVPGMLQRKR 194

Query: 188 GAIVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHVEYKDWGIDVQCQVPLYVATEM 247
           GAIVN+GSGAAV+VPSHPLY IYAATKAYVD+LS SL++EYK++GIDVQ QVPLYVAT+M
Sbjct: 195 GAIVNLGSGAAVVVPSHPLYTIYAATKAYVDKLSTSLYMEYKEYGIDVQIQVPLYVATKM 254

Query: 248 ASRVASVSRASLFIPSADDYVKAAIRQIGYEPRCTPYWAHSLQWCFASLLPEAMLDAWRL 307
            S+VA++ R+SLFIPSAD Y +AA+++IGYE RCTP+WAHSLQW FA L+P+++LDAWRL
Sbjct: 255 VSKVAAIERSSLFIPSADAYAEAAVKRIGYEARCTPFWAHSLQWWFARLVPDSLLDAWRL 314

Query: 308 SIGLERRRK 316
           SIGL RR K
Sbjct: 315 SIGLNRRGK 321

[Close]
BLAST of CSPI05G21810 vs. NCBI nr
Match: gi|596218792|ref|XP_007224023.1| (hypothetical protein PRUPE_ppa015113mg [Prunus persica])

HSP 1 Score: 418.3 bits (1074), Expect = 1.2e-113
Identity = 203/307 (66.12%), Postives = 251/307 (81.76%), Query Frame = 1 

Query: 9   LSTSPIWLLILSFLGFFTILWHSATVARWVYRAFLRPSKDLRYYGSWGIVTGATDGIGKS 68
           L+  PIWLL+LS LGFF  L  + ++A WV+   LRP K+L+ YGSW +VTGATDGIGK+
Sbjct: 11  LTNQPIWLLLLSSLGFFIFLKQATSLANWVFIVLLRPPKNLKRYGSWAMVTGATDGIGKA 70

Query: 69  FAYQLARAGLNLVLVSRSSMKLKAVSKDIQSEFPDTKVKIIELDFTEDDISGGIAEIEEV 128
           FAYQLA+ GLNL+LVSR+S KLK+VSK+IQ+ +P T++K I  DF+   + G    +EE 
Sbjct: 71  FAYQLAQQGLNLILVSRNSAKLKSVSKEIQANYPTTQIKTIAFDFSVGSLGGMNELLEEE 130

Query: 129 IEDLDVGILINNVGITYPNASFFHEVDEKVWMNVFKVNVKGTTWVTKVVLPKMIKKNRGA 188
           I  LDVG+LINNVG+TYP+A F HEVDE+VW NV KVNV+GTT VT  VL  M+++ RGA
Sbjct: 131 IRGLDVGVLINNVGVTYPSARFLHEVDEQVWTNVVKVNVEGTTRVTMAVLRGMVERKRGA 190

Query: 189 IVNIGSGAAVIVPSHPLYAIYAATKAYVDQLSRSLHVEYKDWGIDVQCQVPLYVATEMAS 248
           +VNIGSGA ++VPSHPL+ +YAATKAYVDQLSRSLHVEYK +GIDVQ QVPLYVAT++AS
Sbjct: 191 VVNIGSGAGIVVPSHPLFTVYAATKAYVDQLSRSLHVEYKRYGIDVQSQVPLYVATKLAS 250

Query: 249 RVASVSRASLFIPSADDYVKAAIRQIGYEPRCTPYWAHSLQWCFASLLPEAMLDAWRLSI 308
           +VAS+ R+SLFIP+   Y KAAIR+IGYE RCTP+WAHSLQWC  SL+PE++LDAWRLSI
Sbjct: 251 KVASIERSSLFIPTPHAYAKAAIRRIGYEARCTPFWAHSLQWCLGSLVPESLLDAWRLSI 310

Query: 309 GLERRRK 316
           GL+RR K
Sbjct: 311 GLKRRAK 317

The following BLAST results are available for this feature:
BLAST of CSPI05G21810 vs. Swiss-Prot
Match NameE-valueIdentityDescription
KCR2_ARATH6.2e-9758.22Very-long-chain 3-oxoacyl-CoA reductase-like protein At1g24470 OS=Arabidopsis th... [more]
KCR1_ARATH4.4e-8753.11Very-long-chain 3-oxoacyl-CoA reductase 1 OS=Arabidopsis thaliana GN=KCR1 PE=1 S... [more]
HSDL1_HUMAN2.4e-4839.42Inactive hydroxysteroid dehydrogenase-like protein 1 OS=Homo sapiens GN=HSDL1 PE... [more]
HSDL1_BOVIN4.0e-4838.49Inactive hydroxysteroid dehydrogenase-like protein 1 OS=Bos taurus GN=HSDL1 PE=2... [more]
HSDL1_PONAB2.0e-4738.32Inactive hydroxysteroid dehydrogenase-like protein 1 OS=Pongo abelii GN=HSDL1 PE... [more]
BLAST of CSPI05G21810 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0KS33_CUCSA5.1e-18399.39Uncharacterized protein OS=Cucumis sativus GN=Csa_5G591790 PE=3 SV=1[more]
M5XGG4_PRUPE8.6e-11466.12Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa015113mg PE=3 SV=1[more]
B9RMQ7_RICCO7.3e-11366.99Steroid dehydrogenase, putative OS=Ricinus communis GN=RCOM_1082610 PE=3 SV=1[more]
W9S1A9_9ROSA1.6e-11266.45Inactive hydroxysteroid dehydrogenase-like protein 1 OS=Morus notabilis GN=L484_... [more]
A0A068UXM9_COFCA1.6e-11264.71Uncharacterized protein OS=Coffea canephora GN=GSCOC_T00038162001 PE=4 SV=1[more]
BLAST of CSPI05G21810 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G24470.13.5e-9858.22 beta-ketoacyl reductase 2[more]
AT1G67730.12.5e-8853.11 beta-ketoacyl reductase 1[more]
AT5G10050.18.2e-1531.68 NAD(P)-binding Rossmann-fold superfamily protein[more]
AT3G03330.12.4e-1430.51 NAD(P)-binding Rossmann-fold superfamily protein[more]
AT5G65205.12.0e-1328.02 NAD(P)-binding Rossmann-fold superfamily protein[more]
BLAST of CSPI05G21810 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|449435019|ref|XP_004135293.1|7.3e-18399.39PREDICTED: very-long-chain 3-oxoacyl-CoA reductase-like protein At1g24470 [Cucum... [more]
gi|659090591|ref|XP_008446096.1|7.6e-17293.62PREDICTED: very-long-chain 3-oxoacyl-CoA reductase-like protein At1g24470 [Cucum... [more]
gi|1009153207|ref|XP_015894513.1|8.8e-12067.65PREDICTED: very-long-chain 3-oxoacyl-CoA reductase-like protein At1g24470 [Zizip... [more]
gi|470148953|ref|XP_004310011.1|2.4e-11767.96PREDICTED: very-long-chain 3-oxoacyl-CoA reductase-like protein At1g24470 [Fraga... [more]
gi|596218792|ref|XP_007224023.1|1.2e-11366.12hypothetical protein PRUPE_ppa015113mg [Prunus persica][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR002347SDR_fam
IPR016040NAD(P)-bd_dom
IPR020904Sc_DH/Rdtase_CS
Vocabulary: Molecular Function
TermDefinition
GO:0016491oxidoreductase activity
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008209 androgen metabolic process
biological_process GO:0046520 sphingoid biosynthetic process
biological_process GO:0008150 biological_process
biological_process GO:0008210 estrogen metabolic process
biological_process GO:0016126 sterol biosynthetic process
biological_process GO:0042761 very long-chain fatty acid biosynthetic process
biological_process GO:0009793 embryo development ending in seed dormancy
biological_process GO:0055114 oxidation-reduction process
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0016020 membrane
cellular_component GO:0005575 cellular_component
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0045703 ketoreductase activity
molecular_function GO:0004303 estradiol 17-beta-dehydrogenase activity
molecular_function GO:0018454 acetoacetyl-CoA reductase activity
molecular_function GO:0016491 oxidoreductase activity
molecular_function GO:0016614 oxidoreductase activity, acting on CH-OH group of donors
molecular_function GO:0003674 molecular_function
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI05G21810.1CSPI05G21810.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00080SDRFAMILYcoord: 132..143
score: 3.7E-6coord: 187..195
score: 3.7E-6coord: 209..228
score: 3.
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00081GDHRDHcoord: 132..143
score: 5.2E-17coord: 181..197
score: 5.2E-17coord: 55..72
score: 5.2E-17coord: 209..228
score: 5.2E-17coord: 230..247
score: 5.2
IPR002347Short-chain dehydrogenase/reductase SDRPIRPIRSF00012611-beta-HSD1coord: 6..318
score: 4.7
IPR002347Short-chain dehydrogenase/reductase SDRPANTHERPTHR24322FAMILY NOT NAMEDcoord: 209..240
score: 2.2E-76coord: 58..191
score: 2.2
IPR002347Short-chain dehydrogenase/reductase SDRPFAMPF00106adh_shortcoord: 57..250
score: 8.6
IPR016040NAD(P)-binding domainGENE3DG3DSA:3.40.50.720coord: 53..252
score: 1.9
IPR016040NAD(P)-binding domainunknownSSF51735NAD(P)-binding Rossmann-fold domainscoord: 51..308
score: 2.07
IPR020904Short-chain dehydrogenase/reductase, conserved sitePROSITEPS00061ADH_SHORTcoord: 196..224
scor
NoneNo IPR availablePANTHERPTHR24322:SF552SUBFAMILY NOT NAMEDcoord: 58..191
score: 2.2E-76coord: 209..240
score: 2.2

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
CSPI05G21810Cla022721Watermelon (97103) v1cpiwmB382
CSPI05G21810Cla010003Watermelon (97103) v1cpiwmB410
CSPI05G21810Csa2G350250Cucumber (Chinese Long) v2cpicuB220
CSPI05G21810Csa5G591790Cucumber (Chinese Long) v2cpicuB239
CSPI05G21810MELO3C010710Melon (DHL92) v3.5.1cpimeB389
CSPI05G21810MELO3C011901Melon (DHL92) v3.5.1cpimeB366
CSPI05G21810ClCG08G017490Watermelon (Charleston Gray)cpiwcgB429
CSPI05G21810Lsi08G016240Bottle gourd (USVL1VR-Ls)cpilsiB392
CSPI05G21810Lsi04G006390Bottle gourd (USVL1VR-Ls)cpilsiB372
CSPI05G21810MELO3C011901.2Melon (DHL92) v3.6.1cpimedB359
CSPI05G21810MELO3C010710.2Melon (DHL92) v3.6.1cpimedB383
CSPI05G21810CsaV3_2G025130Cucumber (Chinese Long) v3cpicucB261
CSPI05G21810CsaV3_5G030680Cucumber (Chinese Long) v3cpicucB284
CSPI05G21810Cla97C05G108360Watermelon (97103) v2cpiwmbB402
CSPI05G21810Cla97C08G161190Watermelon (97103) v2cpiwmbB421
CSPI05G21810Bhi04G000507Wax gourdcpiwgoB491
CSPI05G21810Bhi07G001488Wax gourdcpiwgoB516
CSPI05G21810Cucsa.075320Cucumber (Gy14) v1cgycpiB076
CSPI05G21810Cucsa.394680Cucumber (Gy14) v1cgycpiB580
CSPI05G21810CmaCh18G007280Cucurbita maxima (Rimu)cmacpiB419
CSPI05G21810CmaCh04G017990Cucurbita maxima (Rimu)cmacpiB758
CSPI05G21810CmaCh05G000600Cucurbita maxima (Rimu)cmacpiB814
CSPI05G21810CmoCh04G019000Cucurbita moschata (Rifu)cmocpiB748
CSPI05G21810CmoCh12G000380Cucurbita moschata (Rifu)cmocpiB176
CSPI05G21810CmoCh18G007140Cucurbita moschata (Rifu)cmocpiB407
CSPI05G21810CmoCh05G000670Cucurbita moschata (Rifu)cmocpiB797
CSPI05G21810Cp4.1LG11g00350Cucurbita pepo (Zucchini)cpecpiB116
CSPI05G21810Cp4.1LG09g06250Cucurbita pepo (Zucchini)cpecpiB042
CSPI05G21810CsGy2G017500Cucumber (Gy14) v2cgybcpiB077
CSPI05G21810Carg27654Silver-seed gourdcarcpiB1106
CSPI05G21810Carg21850Silver-seed gourdcarcpiB0538
CSPI05G21810Carg14292Silver-seed gourdcarcpiB0275
The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
CSPI05G21810CSPI02G17530Wild cucumber (PI 183967)cpicpiB076
The following block(s) are covering this gene:
GeneOrganismBlock
CSPI05G21810Cucurbita maxima (Rimu)cmacpiB190
CSPI05G21810Melon (DHL92) v3.5.1cpimeB369
CSPI05G21810Watermelon (Charleston Gray)cpiwcgB412
CSPI05G21810Cucurbita pepo (Zucchini)cpecpiB440
CSPI05G21810Cucurbita pepo (Zucchini)cpecpiB837
CSPI05G21810Melon (DHL92) v3.6.1cpimedB363
CSPI05G21810Cucumber (Gy14) v2cgybcpiB225
CSPI05G21810Silver-seed gourdcarcpiB0657