CSPI05G20310 (gene) Wild cucumber (PI 183967)

Overview
NameCSPI05G20310
Typegene
OrganismCucumis sativus (Wild cucumber (PI 183967))
DescriptionGRAM domain-containing protein / ABA-responsive protein-related
LocationChr5 : 21431771 .. 21432736 (-)
Genome Viewer
Gene Expression
   



Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTTTATTTTGGTTCGAAAAAAAATGAAAACTACAATTCAAGAACATATGTTGGGATTTCCAATGTCATTGGCTTTGTCACGGGCTAGATATTTGAGTCATCCTTCGAATCGTCTTCATTTATCTTCTTCAGATGATGGATTTCAATCGTCAATTAAAAAATGTAAGAGTGAAGAAAAAAAAAATATTTCATTTTGGTTGAATATATATAAAATAGTTTGCTAACTAAATTTGGTTTTTGAGTTACTTGTAGATAATGGAAAAGATTGTATCTTGGATAGGCTAAACAAAAATGGAAAGAAAACGGACAATATCATTCATGCGCTCCGAGAACATGGTAAGTCTTTTTTTATAGATATCATCGATCTGGTACATTATGCAAAGTGTAATTAATTTTGATGATTTGAGGTTGAATTTTGCAGTGAAATTAGGAGCCAAGATATCAGAAACAGTGAAGGGAAAGTTGAGCTTAGGAGCAAGAATATTGAGAGTTGGTGGGGTAAGGAAAATCTACAAGAAATTATTTTCAATGAGCGAAGAAGAGAAGCTTTTGAAGGTTTCACAATGCTATTTATCAACAACAGCTGGTCCTTTACCTGGTCTTCTCTTCATTTCCACTCATAAAATTGCTTTTTGTAGTGACAAATCCATCAAAATCGCTTCCCCAAATGGAGATCATATCAGAATTCACTATAAAGTAAGTCTCTAATTTATATATACATACACTAAAATAACTGATCAGTTTTTAATTTGGGTAATTAAAATTTTCATTAGTACTGATGAAGGTTAATTAATGTTTGCATTACAGGTTGTGATTCCAAAGGAGAAGGTGATGAGAGTAAATGAAAGCGAGAATGTAAAGAAAACATCAGAAAGATACATACAAATAGAGACATTGGATAACTTTGAATTTTGGTTTATGGGATTTCTAAATTATCAATCAACTTTCAACTCTCTACAGTGGTAG

mRNA sequence

ATGAAAACTACAATTCAAGAACATATGTTGGGATTTCCAATGTCATTGGCTTTGTCACGGGCTAGATATTTGAGTCATCCTTCGAATCGTCTTCATTTATCTTCTTCAGATGATGGATTTCAATCGTCAATTAAAAAATATAATGGAAAAGATTGTATCTTGGATAGGCTAAACAAAAATGGAAAGAAAACGGACAATATCATTCATGCGCTCCGAGAACATGTGAAATTAGGAGCCAAGATATCAGAAACAGTGAAGGGAAAGTTGAGCTTAGGAGCAAGAATATTGAGAGTTGGTGGGGTAAGGAAAATCTACAAGAAATTATTTTCAATGAGCGAAGAAGAGAAGCTTTTGAAGGTTTCACAATGCTATTTATCAACAACAGCTGGTCCTTTACCTGGTCTTCTCTTCATTTCCACTCATAAAATTGCTTTTTGTAGTGACAAATCCATCAAAATCGCTTCCCCAAATGGAGATCATATCAGAATTCACTATAAAGTTGTGATTCCAAAGGAGAAGGTGATGAGAGTAAATGAAAGCGAGAATGTAAAGAAAACATCAGAAAGATACATACAAATAGAGACATTGGATAACTTTGAATTTTGGTTTATGGGATTTCTAAATTATCAATCAACTTTCAACTCTCTACAGTGGTAG

Coding sequence (CDS)

ATGAAAACTACAATTCAAGAACATATGTTGGGATTTCCAATGTCATTGGCTTTGTCACGGGCTAGATATTTGAGTCATCCTTCGAATCGTCTTCATTTATCTTCTTCAGATGATGGATTTCAATCGTCAATTAAAAAATATAATGGAAAAGATTGTATCTTGGATAGGCTAAACAAAAATGGAAAGAAAACGGACAATATCATTCATGCGCTCCGAGAACATGTGAAATTAGGAGCCAAGATATCAGAAACAGTGAAGGGAAAGTTGAGCTTAGGAGCAAGAATATTGAGAGTTGGTGGGGTAAGGAAAATCTACAAGAAATTATTTTCAATGAGCGAAGAAGAGAAGCTTTTGAAGGTTTCACAATGCTATTTATCAACAACAGCTGGTCCTTTACCTGGTCTTCTCTTCATTTCCACTCATAAAATTGCTTTTTGTAGTGACAAATCCATCAAAATCGCTTCCCCAAATGGAGATCATATCAGAATTCACTATAAAGTTGTGATTCCAAAGGAGAAGGTGATGAGAGTAAATGAAAGCGAGAATGTAAAGAAAACATCAGAAAGATACATACAAATAGAGACATTGGATAACTTTGAATTTTGGTTTATGGGATTTCTAAATTATCAATCAACTTTCAACTCTCTACAGTGGTAG
Homology
[Close]
BLAST of CSPI05G20310 vs. Swiss-Prot
Match: GEML4_ARATH (GEM-like protein 4 OS=Arabidopsis thaliana GN=At5g08350 PE=2 SV=1)

HSP 1 Score: 202.6 bits (514), Expect = 4.4e-51
Identity = 109/216 (50.46%), Postives = 154/216 (71.30%), Query Frame = 1 

Query: 5   IQEHMLGFPMSLALSRARYLSHPS--NRLHL-SSSDDGFQSSIKKYNGKDCILDRLNKNG 64
           +++ ++ +P + A +   YL  P+  N+  + +SS    QS++K       IL R     
Sbjct: 6   VEQQVIRYPAAKA-TPVGYLPDPASFNKFRVPASSKKSEQSNVKS------ILKR----- 65

Query: 65  KKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLLKVS 124
           KKTD   + +R+  K+  K++ETVK KLSLGARIL+VGG+ KI+K+LF +SE EKL K+ 
Sbjct: 66  KKTDGFTNGVRDQSKIRPKLTETVKRKLSLGARILQVGGLEKIFKRLFRVSEGEKLFKMY 125

Query: 125 QCYLSTTAGPLPGLLFISTHKIAFCSDKSIKIASPNGDHIRIHYKVVIPKEKVMRVNESE 184
           QCYLSTTAGP+ GLLFIS+ K+AFCS++SIK+ SP GD IR+HYKV IP  K+ RVN+S+
Sbjct: 126 QCYLSTTAGPIAGLLFISSKKMAFCSERSIKVDSPQGDIIRVHYKVSIPLCKIDRVNQSQ 185

Query: 185 NVKKTSERYIQIETLDNFEFWFMGFLNYQSTFNSLQ 218
           N KK S++Y+++ T+D F+FWFMGFL+YQ  FN L+
Sbjct: 186 NTKKPSQKYLEVVTVDGFDFWFMGFLSYQKAFNCLE 209

[Close]
BLAST of CSPI05G20310 vs. Swiss-Prot
Match: GEML8_ARATH (Putative GEM-like protein 8 OS=Arabidopsis thaliana GN=At5g23370 PE=3 SV=1)

HSP 1 Score: 201.8 bits (512), Expect = 7.6e-51
Identity = 107/215 (49.77%), Postives = 150/215 (69.77%), Query Frame = 1 

Query: 5   IQEHMLGFPMSLALSRARYLSHPS--NRLHLSSSDDGFQSSIKKYNGKDCILDRLNKNGK 64
           + + ++ FP ++  S A YL  P+  N+L + +S    + S     GK  +        K
Sbjct: 6   VHQQLITFP-AVKTSPAGYLPDPASINKLQIPTSS---KFSFLTGKGKSML------RKK 65

Query: 65  KTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLLKVSQ 124
           K D+  + +R+  KLG K++ETVK KLSLGARIL++GG+ KIYK+LF +S+EEKL K  Q
Sbjct: 66  KNDSFTNGVRDQDKLGPKLTETVKRKLSLGARILQMGGLEKIYKRLFKVSDEEKLFKAYQ 125

Query: 125 CYLSTTAGPLPGLLFISTHKIAFCSDKSIKIASPNGDHIRIHYKVVIPKEKVMRVNESEN 184
           CYLSTTAGP+ GLLFIS+ KIAFCS++SIK+ASP G+  R+HYKV IP  K+  VN+S+N
Sbjct: 126 CYLSTTAGPIAGLLFISSKKIAFCSERSIKVASPQGELNRVHYKVSIPLCKINGVNQSQN 185

Query: 185 VKKTSERYIQIETLDNFEFWFMGFLNYQSTFNSLQ 218
             K S++Y+++ T+D F+FWFMGFL+YQ  FN L+
Sbjct: 186 TTKPSQKYLEVVTVDGFDFWFMGFLSYQKAFNCLE 210

[Close]
BLAST of CSPI05G20310 vs. Swiss-Prot
Match: GEML6_ARATH (GEM-like protein 6 OS=Arabidopsis thaliana GN=At5g23350 PE=2 SV=2)

HSP 1 Score: 199.1 bits (505), Expect = 4.9e-50
Identity = 94/156 (60.26%), Postives = 125/156 (80.13%), Query Frame = 1 

Query: 62  KKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLLKVS 121
           KKTD+  +  R+  KLG K++ETVK KLSLGA+IL++GG+ KIYK+LF + ++EKL K  
Sbjct: 55  KKTDSFTNGARDQDKLGPKLTETVKRKLSLGAKILQMGGLEKIYKRLFKVCDQEKLFKAY 114

Query: 122 QCYLSTTAGPLPGLLFISTHKIAFCSDKSIKIASPNGDHIRIHYKVVIPKEKVMRVNESE 181
           QCYLSTTAGP+ GLLFIS+ KIAFCS++SIK+ASP G   R+HYKV IP  K+  VN+S+
Sbjct: 115 QCYLSTTAGPIAGLLFISSKKIAFCSERSIKVASPQGVLSRVHYKVSIPLCKINGVNQSQ 174

Query: 182 NVKKTSERYIQIETLDNFEFWFMGFLNYQSTFNSLQ 218
           N KK S++Y++I T+DNF+FWFMGF++YQ  FN L+
Sbjct: 175 NTKKPSQKYLEIVTIDNFDFWFMGFVSYQKAFNCLE 210

[Close]
BLAST of CSPI05G20310 vs. Swiss-Prot
Match: GEML7_ARATH (GEM-like protein 7 OS=Arabidopsis thaliana GN=At5g23360 PE=2 SV=1)

HSP 1 Score: 195.3 bits (495), Expect = 7.1e-49
Identity = 91/156 (58.33%), Postives = 123/156 (78.85%), Query Frame = 1 

Query: 62  KKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLLKVS 121
           KKTD+  +  R+  KLG K++ETVK KLSLGA+IL++GG+ KIYK+LF + ++EKL K  
Sbjct: 47  KKTDSFTNGARDQDKLGPKLTETVKRKLSLGAKILQMGGLEKIYKRLFKVCDKEKLFKAY 106

Query: 122 QCYLSTTAGPLPGLLFISTHKIAFCSDKSIKIASPNGDHIRIHYKVVIPKEKVMRVNESE 181
           QCYLSTT G + GLLFIS+ KIAFCS++SIK+ SP GD  R+HYKV IP  K+  VN+S+
Sbjct: 107 QCYLSTTEGSIAGLLFISSKKIAFCSERSIKVTSPQGDLTRVHYKVSIPLCKINGVNQSQ 166

Query: 182 NVKKTSERYIQIETLDNFEFWFMGFLNYQSTFNSLQ 218
           N KK S+RY+++ T+DN++FWFMGF++YQ  FN L+
Sbjct: 167 NTKKPSQRYLEVVTVDNYDFWFMGFVSYQKAFNCLE 202

[Close]
BLAST of CSPI05G20310 vs. Swiss-Prot
Match: GEML2_ARATH (GEM-like protein 2 OS=Arabidopsis thaliana GN=At4g01600 PE=2 SV=1)

HSP 1 Score: 140.6 bits (353), Expect = 2.1e-32
Identity = 71/183 (38.80%), Postives = 115/183 (62.84%), Query Frame = 1 

Query: 42  SSIKKYNGKDCILDRLNKNGKKTDN-------IIHALREHVKLGAKISETVKGKLSLGAR 101
           +S      KD +L+ LN+ GKK ++       ++  L++H+K    IS+    +LS G +
Sbjct: 38  TSASDKRSKDKVLEVLNRCGKKVEDATRKAEALVGGLKDHLKFSPSISDAAMARLSQGTK 97

Query: 102 ILRVGGVRKIYKKLFSMSEEEKLLKVSQCYLSTTAGPLPGLLFISTHKIAFCSDKSIKI- 161
           ++  GG  +++++ F +   EKLL    CY+STT+GP+ G+++IS  +IAFCSD +I++ 
Sbjct: 98  MIVEGGPERVFQREFGVLAVEKLLDSFVCYISTTSGPVTGVIYISNRRIAFCSDYAIRLP 157

Query: 162 ASPNGDHIRIHYKVVIPKEKVMRVNESENVKKTSERYIQIETLDNFEFWFMGFLNYQSTF 217
           +S  G+ +  +YKVV+  EK+  ++ S NV K SERY+ + T D FEFWFMGF++Y   F
Sbjct: 158 SSAGGNGVAAYYKVVMEWEKISSISSSTNVLKPSERYVHMVTRDGFEFWFMGFVSYIDAF 217

[Close]
BLAST of CSPI05G20310 vs. TrEMBL
Match: A0A0A0KPM2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G579030 PE=4 SV=1)

HSP 1 Score: 403.3 bits (1035), Expect = 1.9e-109
Identity = 204/205 (99.51%), Postives = 204/205 (99.51%), Query Frame = 1 

Query: 14  MSLALSRARYLSHPSNRLHLSSSDDGFQSSIKKYNGKDCILDRLNKNGKKTDNIIHALRE 73
           MSLALSRARYLSHPSNRLHLSSSDDGFQSSIKKYNGKDCIL RLNKNGKKTDNIIHALRE
Sbjct: 1   MSLALSRARYLSHPSNRLHLSSSDDGFQSSIKKYNGKDCILKRLNKNGKKTDNIIHALRE 60

Query: 74  HVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLLKVSQCYLSTTAGPLP 133
           HVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLLKVSQCYLSTTAGPLP
Sbjct: 61  HVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLLKVSQCYLSTTAGPLP 120

Query: 134 GLLFISTHKIAFCSDKSIKIASPNGDHIRIHYKVVIPKEKVMRVNESENVKKTSERYIQI 193
           GLLFISTHKIAFCSDKSIKIASPNGDHIRIHYKVVIPKEKVMRVNESENVKKTSERYIQI
Sbjct: 121 GLLFISTHKIAFCSDKSIKIASPNGDHIRIHYKVVIPKEKVMRVNESENVKKTSERYIQI 180

Query: 194 ETLDNFEFWFMGFLNYQSTFNSLQW 219
           ETLDNFEFWFMGFLNYQSTFNSLQW
Sbjct: 181 ETLDNFEFWFMGFLNYQSTFNSLQW 205

[Close]
BLAST of CSPI05G20310 vs. TrEMBL
Match: A0A0A0KT32_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G579000 PE=4 SV=1)

HSP 1 Score: 375.9 bits (964), Expect = 3.3e-101
Identity = 195/205 (95.12%), Postives = 196/205 (95.61%), Query Frame = 1 

Query: 14  MSLALSRARYLSHPSNRLHLSSSDDGF-QSSIKKYNGKDCILDRLNKNGKKTDNIIHALR 73
           MSLALSRAR L H S  LH+SSSDD   QSSIKKYNGKDCIL RLNKNGKKTDNIIHALR
Sbjct: 1   MSLALSRARSLGHSSKHLHVSSSDDRISQSSIKKYNGKDCILKRLNKNGKKTDNIIHALR 60

Query: 74  EHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLLKVSQCYLSTTAGPL 133
           EHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLLKVSQCYLSTTAGPL
Sbjct: 61  EHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLLKVSQCYLSTTAGPL 120

Query: 134 PGLLFISTHKIAFCSDKSIKIASPNGDHIRIHYKVVIPKEKVMRVNESENVKKTSERYIQ 193
           PGLLFISTHKIAFCSDKSIKIASPNGDHIRIHYKVVIPKEKVMRVNESENVKKTSERYIQ
Sbjct: 121 PGLLFISTHKIAFCSDKSIKIASPNGDHIRIHYKVVIPKEKVMRVNESENVKKTSERYIQ 180

Query: 194 IETLDNFEFWFMGFLNYQSTFNSLQ 218
           IETLDNFEFWFMGFLNYQSTFNSLQ
Sbjct: 181 IETLDNFEFWFMGFLNYQSTFNSLQ 205

[Close]
BLAST of CSPI05G20310 vs. TrEMBL
Match: A0A0A0KV22_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G578990 PE=4 SV=1)

HSP 1 Score: 348.6 bits (893), Expect = 5.6e-93
Identity = 183/219 (83.56%), Postives = 195/219 (89.04%), Query Frame = 1 

Query: 1   MKTTIQEHM-LGFPM-SLALSRARYLSHPSNRLHLSSSDDGFQSSIKKYNGKDCILDRLN 60
           MKTTIQE + LG  M SL L+RAR L  PS  LH+SSSDDG Q SIKKYNGKDCIL+RLN
Sbjct: 1   MKTTIQEQIVLGIQMKSLGLARARLLGDPSKYLHISSSDDGSQ-SIKKYNGKDCILNRLN 60

Query: 61  KNGKKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLL 120
           KNGKKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLL
Sbjct: 61  KNGKKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLL 120

Query: 121 KVSQCYLSTTAGPLPGLLFISTHKIAFCSDKSIKIASPNGDHIRIHYKVVIPKEKVMRVN 180
           KVSQCYLSTTAGPLPGLLFISTHKIAFCSDKSIKIASPNGDHIRIHYKV IP  K+ RV 
Sbjct: 121 KVSQCYLSTTAGPLPGLLFISTHKIAFCSDKSIKIASPNGDHIRIHYKVTIPLGKITRVF 180

Query: 181 ESENVKKTSERYIQIETLDNFEFWFMGFLNYQSTFNSLQ 218
           +SENVK  SE+Y++I T+DN+EFWFMGFLNY  +FN LQ
Sbjct: 181 QSENVKNPSEKYMEIVTVDNYEFWFMGFLNYHKSFNCLQ 218

[Close]
BLAST of CSPI05G20310 vs. TrEMBL
Match: A0A0A0KRR9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G579020 PE=4 SV=1)

HSP 1 Score: 293.9 bits (751), Expect = 1.6e-76
Identity = 161/226 (71.24%), Postives = 184/226 (81.42%), Query Frame = 1 

Query: 1   MKTTIQEHMLGFPMSLALSRARYLSHPSNRLHLSSSDDGFQSSIKKYNGKDCILDRLNKN 60
           MKTTIQEHML F M+LAL++ARYL H S  L +SSS++G   S+KK NGKD IL+RLNKN
Sbjct: 1   MKTTIQEHMLEFQMTLALAKARYLGHSSKHLDISSSNNG---SLKKGNGKDWILNRLNKN 60

Query: 61  GKKT---DNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKL 120
             KT   D+IIHAL+EHVKLG KISE +KGKL LG R LRVGG+RK+YKKLF M+EEEKL
Sbjct: 61  EGKTDMLDDIIHALQEHVKLGGKISEKIKGKLGLGGRTLRVGGMRKMYKKLFPMNEEEKL 120

Query: 121 LKVSQCYLSTTAGPLPGLLFISTHKIAFCSDKSIKIASP---NGDHIRIHYKVVIPKEKV 180
           LKVSQCYLSTTAGPL GLLF+STHKIAFCS KSI + SP   + D++RIHYKVVIP EKV
Sbjct: 121 LKVSQCYLSTTAGPLGGLLFLSTHKIAFCSAKSITVLSPPNGDNDYVRIHYKVVIPMEKV 180

Query: 181 MRVNESE--NVKKTSERYIQIETLDNFEFWFMGFL-NYQSTFNSLQ 218
           M VNES+  NVK  SE+YIQI T+DN EFWFMG L NYQS FNSL+
Sbjct: 181 MGVNESDQKNVKTDSEKYIQIVTVDNLEFWFMGLLNNYQSIFNSLE 223

[Close]
BLAST of CSPI05G20310 vs. TrEMBL
Match: A0A0A0KMT0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G352650 PE=4 SV=1)

HSP 1 Score: 253.1 bits (645), Expect = 3.2e-64
Identity = 131/222 (59.01%), Postives = 172/222 (77.48%), Query Frame = 1 

Query: 1   MKTTIQEHMLGFPM-SLAL----SRARYLSHPSNRLHLSSSDDGFQSSIKKYNGKDCILD 60
           M+T +Q H++GFP+ S AL    S  R L   + + ++SSSD  + +   K   KD +L 
Sbjct: 1   METLLQRHVIGFPIGSTALQVEQSVQRLLPDLAGQYNISSSDTKYPAL--KQCSKDSMLS 60

Query: 61  RLNKNGKKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEE 120
           R+NK GK+ D+  H +REHV+LG+KISETVKGKLSLGARIL+VGG+RKI+K+LF++ + E
Sbjct: 61  RVNKLGKRADSFAHGVREHVRLGSKISETVKGKLSLGARILQVGGLRKIFKQLFNVGDGE 120

Query: 121 KLLKVSQCYLSTTAGPLPGLLFISTHKIAFCSDKSIKIASPNGDHIRIHYKVVIPKEKVM 180
           KLLK   CYLSTTAGP+ GLLFIST+KIAFCSD+SIK++SP+G+ IR HYKVVIP  K+ 
Sbjct: 121 KLLKAVHCYLSTTAGPIAGLLFISTNKIAFCSDRSIKVSSPSGELIRFHYKVVIPVGKIE 180

Query: 181 RVNESENVKKTSERYIQIETLDNFEFWFMGFLNYQSTFNSLQ 218
           R+N+SENVKK S++Y++I T+DNF+FWFMGFLNY  T   LQ
Sbjct: 181 RINQSENVKKPSQKYMEIVTVDNFDFWFMGFLNYHKTLQYLQ 220

[Close]
BLAST of CSPI05G20310 vs. TAIR10
Match: AT5G08350.1 (AT5G08350.1 GRAM domain-containing protein / ABA-responsive protein-related)

HSP 1 Score: 202.6 bits (514), Expect = 2.5e-52
Identity = 109/216 (50.46%), Postives = 154/216 (71.30%), Query Frame = 1 

Query: 5   IQEHMLGFPMSLALSRARYLSHPS--NRLHL-SSSDDGFQSSIKKYNGKDCILDRLNKNG 64
           +++ ++ +P + A +   YL  P+  N+  + +SS    QS++K       IL R     
Sbjct: 6   VEQQVIRYPAAKA-TPVGYLPDPASFNKFRVPASSKKSEQSNVKS------ILKR----- 65

Query: 65  KKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLLKVS 124
           KKTD   + +R+  K+  K++ETVK KLSLGARIL+VGG+ KI+K+LF +SE EKL K+ 
Sbjct: 66  KKTDGFTNGVRDQSKIRPKLTETVKRKLSLGARILQVGGLEKIFKRLFRVSEGEKLFKMY 125

Query: 125 QCYLSTTAGPLPGLLFISTHKIAFCSDKSIKIASPNGDHIRIHYKVVIPKEKVMRVNESE 184
           QCYLSTTAGP+ GLLFIS+ K+AFCS++SIK+ SP GD IR+HYKV IP  K+ RVN+S+
Sbjct: 126 QCYLSTTAGPIAGLLFISSKKMAFCSERSIKVDSPQGDIIRVHYKVSIPLCKIDRVNQSQ 185

Query: 185 NVKKTSERYIQIETLDNFEFWFMGFLNYQSTFNSLQ 218
           N KK S++Y+++ T+D F+FWFMGFL+YQ  FN L+
Sbjct: 186 NTKKPSQKYLEVVTVDGFDFWFMGFLSYQKAFNCLE 209

[Close]
BLAST of CSPI05G20310 vs. TAIR10
Match: AT5G23370.1 (AT5G23370.1 GRAM domain-containing protein / ABA-responsive protein-related)

HSP 1 Score: 201.8 bits (512), Expect = 4.3e-52
Identity = 107/215 (49.77%), Postives = 150/215 (69.77%), Query Frame = 1 

Query: 5   IQEHMLGFPMSLALSRARYLSHPS--NRLHLSSSDDGFQSSIKKYNGKDCILDRLNKNGK 64
           + + ++ FP ++  S A YL  P+  N+L + +S    + S     GK  +        K
Sbjct: 6   VHQQLITFP-AVKTSPAGYLPDPASINKLQIPTSS---KFSFLTGKGKSML------RKK 65

Query: 65  KTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLLKVSQ 124
           K D+  + +R+  KLG K++ETVK KLSLGARIL++GG+ KIYK+LF +S+EEKL K  Q
Sbjct: 66  KNDSFTNGVRDQDKLGPKLTETVKRKLSLGARILQMGGLEKIYKRLFKVSDEEKLFKAYQ 125

Query: 125 CYLSTTAGPLPGLLFISTHKIAFCSDKSIKIASPNGDHIRIHYKVVIPKEKVMRVNESEN 184
           CYLSTTAGP+ GLLFIS+ KIAFCS++SIK+ASP G+  R+HYKV IP  K+  VN+S+N
Sbjct: 126 CYLSTTAGPIAGLLFISSKKIAFCSERSIKVASPQGELNRVHYKVSIPLCKINGVNQSQN 185

Query: 185 VKKTSERYIQIETLDNFEFWFMGFLNYQSTFNSLQ 218
             K S++Y+++ T+D F+FWFMGFL+YQ  FN L+
Sbjct: 186 TTKPSQKYLEVVTVDGFDFWFMGFLSYQKAFNCLE 210

[Close]
BLAST of CSPI05G20310 vs. TAIR10
Match: AT5G23350.1 (AT5G23350.1 GRAM domain-containing protein / ABA-responsive protein-related)

HSP 1 Score: 199.1 bits (505), Expect = 2.8e-51
Identity = 94/156 (60.26%), Postives = 125/156 (80.13%), Query Frame = 1 

Query: 62  KKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLLKVS 121
           KKTD+  +  R+  KLG K++ETVK KLSLGA+IL++GG+ KIYK+LF + ++EKL K  
Sbjct: 117 KKTDSFTNGARDQDKLGPKLTETVKRKLSLGAKILQMGGLEKIYKRLFKVCDQEKLFKAY 176

Query: 122 QCYLSTTAGPLPGLLFISTHKIAFCSDKSIKIASPNGDHIRIHYKVVIPKEKVMRVNESE 181
           QCYLSTTAGP+ GLLFIS+ KIAFCS++SIK+ASP G   R+HYKV IP  K+  VN+S+
Sbjct: 177 QCYLSTTAGPIAGLLFISSKKIAFCSERSIKVASPQGVLSRVHYKVSIPLCKINGVNQSQ 236

Query: 182 NVKKTSERYIQIETLDNFEFWFMGFLNYQSTFNSLQ 218
           N KK S++Y++I T+DNF+FWFMGF++YQ  FN L+
Sbjct: 237 NTKKPSQKYLEIVTIDNFDFWFMGFVSYQKAFNCLE 272

[Close]
BLAST of CSPI05G20310 vs. TAIR10
Match: AT5G23360.1 (AT5G23360.1 GRAM domain-containing protein / ABA-responsive protein-related)

HSP 1 Score: 195.3 bits (495), Expect = 4.0e-50
Identity = 91/156 (58.33%), Postives = 123/156 (78.85%), Query Frame = 1 

Query: 62  KKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLLKVS 121
           KKTD+  +  R+  KLG K++ETVK KLSLGA+IL++GG+ KIYK+LF + ++EKL K  
Sbjct: 47  KKTDSFTNGARDQDKLGPKLTETVKRKLSLGAKILQMGGLEKIYKRLFKVCDKEKLFKAY 106

Query: 122 QCYLSTTAGPLPGLLFISTHKIAFCSDKSIKIASPNGDHIRIHYKVVIPKEKVMRVNESE 181
           QCYLSTT G + GLLFIS+ KIAFCS++SIK+ SP GD  R+HYKV IP  K+  VN+S+
Sbjct: 107 QCYLSTTEGSIAGLLFISSKKIAFCSERSIKVTSPQGDLTRVHYKVSIPLCKINGVNQSQ 166

Query: 182 NVKKTSERYIQIETLDNFEFWFMGFLNYQSTFNSLQ 218
           N KK S+RY+++ T+DN++FWFMGF++YQ  FN L+
Sbjct: 167 NTKKPSQRYLEVVTVDNYDFWFMGFVSYQKAFNCLE 202

[Close]
BLAST of CSPI05G20310 vs. TAIR10
Match: AT4G01600.1 (AT4G01600.1 GRAM domain family protein)

HSP 1 Score: 140.6 bits (353), Expect = 1.2e-33
Identity = 71/183 (38.80%), Postives = 115/183 (62.84%), Query Frame = 1 

Query: 42  SSIKKYNGKDCILDRLNKNGKKTDN-------IIHALREHVKLGAKISETVKGKLSLGAR 101
           +S      KD +L+ LN+ GKK ++       ++  L++H+K    IS+    +LS G +
Sbjct: 38  TSASDKRSKDKVLEVLNRCGKKVEDATRKAEALVGGLKDHLKFSPSISDAAMARLSQGTK 97

Query: 102 ILRVGGVRKIYKKLFSMSEEEKLLKVSQCYLSTTAGPLPGLLFISTHKIAFCSDKSIKI- 161
           ++  GG  +++++ F +   EKLL    CY+STT+GP+ G+++IS  +IAFCSD +I++ 
Sbjct: 98  MIVEGGPERVFQREFGVLAVEKLLDSFVCYISTTSGPVTGVIYISNRRIAFCSDYAIRLP 157

Query: 162 ASPNGDHIRIHYKVVIPKEKVMRVNESENVKKTSERYIQIETLDNFEFWFMGFLNYQSTF 217
           +S  G+ +  +YKVV+  EK+  ++ S NV K SERY+ + T D FEFWFMGF++Y   F
Sbjct: 158 SSAGGNGVAAYYKVVMEWEKISSISSSTNVLKPSERYVHMVTRDGFEFWFMGFVSYIDAF 217

[Close]
BLAST of CSPI05G20310 vs. NCBI nr
Match: gi|778704224|ref|XP_011655497.1| (PREDICTED: GEM-like protein 4 [Cucumis sativus])

HSP 1 Score: 430.3 bits (1105), Expect = 2.1e-117
Identity = 217/218 (99.54%), Postives = 217/218 (99.54%), Query Frame = 1 

Query: 1   MKTTIQEHMLGFPMSLALSRARYLSHPSNRLHLSSSDDGFQSSIKKYNGKDCILDRLNKN 60
           MKTTIQEHMLGFPMSLALSRARYLSHPSNRLHLSSSDDGFQSSIKKYNGKDCIL RLNKN
Sbjct: 1   MKTTIQEHMLGFPMSLALSRARYLSHPSNRLHLSSSDDGFQSSIKKYNGKDCILKRLNKN 60

Query: 61  GKKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLLKV 120
           GKKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLLKV
Sbjct: 61  GKKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLLKV 120

Query: 121 SQCYLSTTAGPLPGLLFISTHKIAFCSDKSIKIASPNGDHIRIHYKVVIPKEKVMRVNES 180
           SQCYLSTTAGPLPGLLFISTHKIAFCSDKSIKIASPNGDHIRIHYKVVIPKEKVMRVNES
Sbjct: 121 SQCYLSTTAGPLPGLLFISTHKIAFCSDKSIKIASPNGDHIRIHYKVVIPKEKVMRVNES 180

Query: 181 ENVKKTSERYIQIETLDNFEFWFMGFLNYQSTFNSLQW 219
           ENVKKTSERYIQIETLDNFEFWFMGFLNYQSTFNSLQW
Sbjct: 181 ENVKKTSERYIQIETLDNFEFWFMGFLNYQSTFNSLQW 218

[Close]
BLAST of CSPI05G20310 vs. NCBI nr
Match: gi|700196388|gb|KGN51565.1| (hypothetical protein Csa_5G579030 [Cucumis sativus])

HSP 1 Score: 403.3 bits (1035), Expect = 2.7e-109
Identity = 204/205 (99.51%), Postives = 204/205 (99.51%), Query Frame = 1 

Query: 14  MSLALSRARYLSHPSNRLHLSSSDDGFQSSIKKYNGKDCILDRLNKNGKKTDNIIHALRE 73
           MSLALSRARYLSHPSNRLHLSSSDDGFQSSIKKYNGKDCIL RLNKNGKKTDNIIHALRE
Sbjct: 1   MSLALSRARYLSHPSNRLHLSSSDDGFQSSIKKYNGKDCILKRLNKNGKKTDNIIHALRE 60

Query: 74  HVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLLKVSQCYLSTTAGPLP 133
           HVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLLKVSQCYLSTTAGPLP
Sbjct: 61  HVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLLKVSQCYLSTTAGPLP 120

Query: 134 GLLFISTHKIAFCSDKSIKIASPNGDHIRIHYKVVIPKEKVMRVNESENVKKTSERYIQI 193
           GLLFISTHKIAFCSDKSIKIASPNGDHIRIHYKVVIPKEKVMRVNESENVKKTSERYIQI
Sbjct: 121 GLLFISTHKIAFCSDKSIKIASPNGDHIRIHYKVVIPKEKVMRVNESENVKKTSERYIQI 180

Query: 194 ETLDNFEFWFMGFLNYQSTFNSLQW 219
           ETLDNFEFWFMGFLNYQSTFNSLQW
Sbjct: 181 ETLDNFEFWFMGFLNYQSTFNSLQW 205

[Close]
BLAST of CSPI05G20310 vs. NCBI nr
Match: gi|778704232|ref|XP_011655499.1| (PREDICTED: GEM-like protein 4 [Cucumis sativus])

HSP 1 Score: 400.2 bits (1027), Expect = 2.3e-108
Identity = 207/218 (94.95%), Postives = 208/218 (95.41%), Query Frame = 1 

Query: 1   MKTTIQEHMLGFPMSLALSRARYLSHPSNRLHLSSSDDGF-QSSIKKYNGKDCILDRLNK 60
           MKTTIQEHMLGF MSLALSRAR L H S  LH+SSSDD   QSSIKKYNGKDCIL RLNK
Sbjct: 1   MKTTIQEHMLGFQMSLALSRARSLGHSSKHLHVSSSDDRISQSSIKKYNGKDCILKRLNK 60

Query: 61  NGKKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLLK 120
           NGKKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLLK
Sbjct: 61  NGKKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLLK 120

Query: 121 VSQCYLSTTAGPLPGLLFISTHKIAFCSDKSIKIASPNGDHIRIHYKVVIPKEKVMRVNE 180
           VSQCYLSTTAGPLPGLLFISTHKIAFCSDKSIKIASPNGDHIRIHYKVVIPKEKVMRVNE
Sbjct: 121 VSQCYLSTTAGPLPGLLFISTHKIAFCSDKSIKIASPNGDHIRIHYKVVIPKEKVMRVNE 180

Query: 181 SENVKKTSERYIQIETLDNFEFWFMGFLNYQSTFNSLQ 218
           SENVKKTSERYIQIETLDNFEFWFMGFLNYQSTFNSLQ
Sbjct: 181 SENVKKTSERYIQIETLDNFEFWFMGFLNYQSTFNSLQ 218

[Close]
BLAST of CSPI05G20310 vs. NCBI nr
Match: gi|659090211|ref|XP_008445894.1| (PREDICTED: GEM-like protein 4 [Cucumis melo])

HSP 1 Score: 397.9 bits (1021), Expect = 1.1e-107
Identity = 201/218 (92.20%), Postives = 207/218 (94.95%), Query Frame = 1 

Query: 1   MKTTIQEHMLGFPMSLALSRARYLSHPSNRLHLSSSDDGFQSSIKKYNGKDCILDRLNKN 60
           MKTTIQEHMLGF MSLALSRARYL HP  RLHLSSSDDG QSSIKKYNGKDCIL+RLN N
Sbjct: 1   MKTTIQEHMLGFQMSLALSRARYLGHPLKRLHLSSSDDGSQSSIKKYNGKDCILNRLNTN 60

Query: 61  GKKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLLKV 120
           GK+TDNIIHALREHVKLG KISE VKGKLSLGA+ILRVGG+RKIYKKLFSM+EEEKLLKV
Sbjct: 61  GKRTDNIIHALREHVKLGGKISEAVKGKLSLGAKILRVGGLRKIYKKLFSMNEEEKLLKV 120

Query: 121 SQCYLSTTAGPLPGLLFISTHKIAFCSDKSIKIASPNGDHIRIHYKVVIPKEKVMRVNES 180
           SQCYLSTTAGPL GLLFISTHKIAFCSDKSIKIASPNGDHIRI YKVVIPKEKVMRVNES
Sbjct: 121 SQCYLSTTAGPLAGLLFISTHKIAFCSDKSIKIASPNGDHIRILYKVVIPKEKVMRVNES 180

Query: 181 ENVKKTSERYIQIETLDNFEFWFMGFLNYQSTFNSLQW 219
           ENVKK SE+YIQIETLDNFEFWFMGFLNYQSTFNSLQW
Sbjct: 181 ENVKKASEKYIQIETLDNFEFWFMGFLNYQSTFNSLQW 218

[Close]
BLAST of CSPI05G20310 vs. NCBI nr
Match: gi|659131076|ref|XP_008465496.1| (PREDICTED: GEM-like protein 4 [Cucumis melo])

HSP 1 Score: 385.6 bits (989), Expect = 5.9e-104
Identity = 202/218 (92.66%), Postives = 206/218 (94.50%), Query Frame = 1 

Query: 3   TTIQEHMLGFPMSLALSRARYLSH-PSNRLHLSSSDDGF-QSSIKKYNGKDCILDRLNKN 62
           TTIQEHMLGF MS ALSRARYL H PS  LH+SSSDD   QSSIKKYNGKDCIL+RLNKN
Sbjct: 4   TTIQEHMLGFQMSFALSRARYLGHYPSKHLHVSSSDDRISQSSIKKYNGKDCILNRLNKN 63

Query: 63  GKKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEKLLKV 122
           GKKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSM+EEEKLLKV
Sbjct: 64  GKKTDNIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMNEEEKLLKV 123

Query: 123 SQCYLSTTAGPLPGLLFISTHKIAFCSDKSIKIASP-NGDHIRIHYKVVIPKEKVMRVNE 182
           SQCYLSTTAGPL GLLFISTHKIAFCSDKSIKIASP NGDHIRIHYKVVIPKEKVMRVNE
Sbjct: 124 SQCYLSTTAGPLAGLLFISTHKIAFCSDKSIKIASPNNGDHIRIHYKVVIPKEKVMRVNE 183

Query: 183 SENVKKTSERYIQIETLDNFEFWFMGFLNYQSTFNSLQ 218
           SENVKK SE+YIQIETLDNFEFWFMGFLNYQSTFNSLQ
Sbjct: 184 SENVKKASEKYIQIETLDNFEFWFMGFLNYQSTFNSLQ 221

The following BLAST results are available for this feature:
BLAST of CSPI05G20310 vs. Swiss-Prot
Match NameE-valueIdentityDescription
GEML4_ARATH4.4e-5150.46GEM-like protein 4 OS=Arabidopsis thaliana GN=At5g08350 PE=2 SV=1[more]
GEML8_ARATH7.6e-5149.77Putative GEM-like protein 8 OS=Arabidopsis thaliana GN=At5g23370 PE=3 SV=1[more]
GEML6_ARATH4.9e-5060.26GEM-like protein 6 OS=Arabidopsis thaliana GN=At5g23350 PE=2 SV=2[more]
GEML7_ARATH7.1e-4958.33GEM-like protein 7 OS=Arabidopsis thaliana GN=At5g23360 PE=2 SV=1[more]
GEML2_ARATH2.1e-3238.80GEM-like protein 2 OS=Arabidopsis thaliana GN=At4g01600 PE=2 SV=1[more]
BLAST of CSPI05G20310 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0KPM2_CUCSA1.9e-10999.51Uncharacterized protein OS=Cucumis sativus GN=Csa_5G579030 PE=4 SV=1[more]
A0A0A0KT32_CUCSA3.3e-10195.12Uncharacterized protein OS=Cucumis sativus GN=Csa_5G579000 PE=4 SV=1[more]
A0A0A0KV22_CUCSA5.6e-9383.56Uncharacterized protein OS=Cucumis sativus GN=Csa_5G578990 PE=4 SV=1[more]
A0A0A0KRR9_CUCSA1.6e-7671.24Uncharacterized protein OS=Cucumis sativus GN=Csa_5G579020 PE=4 SV=1[more]
A0A0A0KMT0_CUCSA3.2e-6459.01Uncharacterized protein OS=Cucumis sativus GN=Csa_5G352650 PE=4 SV=1[more]
BLAST of CSPI05G20310 vs. TAIR10
Match NameE-valueIdentityDescription
AT5G08350.12.5e-5250.46 GRAM domain-containing protein / ABA-responsive protein-related[more]
AT5G23370.14.3e-5249.77 GRAM domain-containing protein / ABA-responsive protein-related[more]
AT5G23350.12.8e-5160.26 GRAM domain-containing protein / ABA-responsive protein-related[more]
AT5G23360.14.0e-5058.33 GRAM domain-containing protein / ABA-responsive protein-related[more]
AT4G01600.11.2e-3338.80 GRAM domain family protein[more]
BLAST of CSPI05G20310 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|778704224|ref|XP_011655497.1|2.1e-11799.54PREDICTED: GEM-like protein 4 [Cucumis sativus][more]
gi|700196388|gb|KGN51565.1|2.7e-10999.51hypothetical protein Csa_5G579030 [Cucumis sativus][more]
gi|778704232|ref|XP_011655499.1|2.3e-10894.95PREDICTED: GEM-like protein 4 [Cucumis sativus][more]
gi|659090211|ref|XP_008445894.1|1.1e-10792.20PREDICTED: GEM-like protein 4 [Cucumis melo][more]
gi|659131076|ref|XP_008465496.1|5.9e-10492.66PREDICTED: GEM-like protein 4 [Cucumis melo][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR004182GRAM
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0003674 molecular_function
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI05G20310.1CSPI05G20310.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004182GRAM domainPFAMPF02893GRAMcoord: 105..179
score: 5.1
IPR004182GRAM domainSMARTSM00568gram2001ccoord: 103..181
score: 6.4
NoneNo IPR availablePANTHERPTHR31969FAMILY NOT NAMEDcoord: 1..217
score: 1.4E
NoneNo IPR availablePANTHERPTHR31969:SF1GEM-LIKE PROTEIN 4-RELATEDcoord: 1..217
score: 1.4E

Synteny