CSPI05G08400 (gene) Wild cucumber (PI 183967) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CSPI05G08400
Type	gene
Organism	Cucumis sativus (Wild cucumber (PI 183967))
Description	Cytochrome P450
Location	Chr5 : 7111979 .. 7112614 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTGATACCTTCCTTTTCCATTCTTCTTCCATCTCTTCTTCTTCTTCTTCTACCTTCAGTTTTCATTATTCTCAAACACATCAAATCTCCATCTTCAAAAAAACTCCCACTTCCTCCTCGTCCAAATCCATGGCCAATCTTAGGAAGTCTCTTTCACATTGGTCAAAATGCTCACATTTCCATCACCCAATTTGCCAATATCTATGGCCCTTTAATCTCCCTCAAGCTCGGAGCTCAGCGTCTCATCGTCGCTTCTTCTCGGGCTGCCACCACTGCAGTTCTCAGGACGCAAGACTGCCTTCTCTCTGCTCGATACATCTTTCAAATGACCCCTGATAGAGCTCTCCACGATCAGCACTCTCTTGTTTTCTCCCCTGAATATGGAGACCAATGGAAAAATTTGAGGTCAATTTGTAAAGTGAACTTGTTTACGGCTAAAGAAATTGAATCACAGACTACTTTGAGAGAGAAGAAGATGAAGGAGTTAGTGGAATTTCTGGAATCAAAACAAGGATCTGTTGTGGAGATTAAAGATTTTGTTTTTACCTCTGTTTTCAAATCTCTTACTAACTTGATACAAAATCAAGTTAGAAAGCCAACAAACTTATTTCCAATTAAACTCACTTATATCTGA

mRNA sequence

ATGTTGATACCTTCCTTTTCCATTCTTCTTCCATCTCTTCTTCTTCTTCTTCTACCTTCAGTTTTCATTATTCTCAAACACATCAAATCTCCATCTTCAAAAAAACTCCCACTTCCTCCTCGTCCAAATCCATGGCCAATCTTAGGAAGTCTCTTTCACATTGGTCAAAATGCTCACATTTCCATCACCCAATTTGCCAATATCTATGGCCCTTTAATCTCCCTCAAGCTCGGAGCTCAGCGTCTCATCGTCGCTTCTTCTCGGGCTGCCACCACTGCAGTTCTCAGGACGCAAGACTGCCTTCTCTCTGCTCGATACATCTTTCAAATGACCCCTGATAGAGCTCTCCACGATCAGCACTCTCTTGTTTTCTCCCCTGAATATGGAGACCAATGGAAAAATTTGAGGTCAATTTGTAAAGTGAACTTGTTTACGGCTAAAGAAATTGAATCACAGACTACTTTGAGAGAGAAGAAGATGAAGGAGTTAGTGGAATTTCTGGAATCAAAACAAGGATCTGTTGTGGAGATTAAAGATTTTGTTTTTACCTCTGTTTTCAAATCTCTTACTAACTTGATACAAAATCAAGTTAGAAAGCCAACAAACTTATTTCCAATTAAACTCACTTATATCTGA

Coding sequence (CDS)

ATGTTGATACCTTCCTTTTCCATTCTTCTTCCATCTCTTCTTCTTCTTCTTCTACCTTCAGTTTTCATTATTCTCAAACACATCAAATCTCCATCTTCAAAAAAACTCCCACTTCCTCCTCGTCCAAATCCATGGCCAATCTTAGGAAGTCTCTTTCACATTGGTCAAAATGCTCACATTTCCATCACCCAATTTGCCAATATCTATGGCCCTTTAATCTCCCTCAAGCTCGGAGCTCAGCGTCTCATCGTCGCTTCTTCTCGGGCTGCCACCACTGCAGTTCTCAGGACGCAAGACTGCCTTCTCTCTGCTCGATACATCTTTCAAATGACCCCTGATAGAGCTCTCCACGATCAGCACTCTCTTGTTTTCTCCCCTGAATATGGAGACCAATGGAAAAATTTGAGGTCAATTTGTAAAGTGAACTTGTTTACGGCTAAAGAAATTGAATCACAGACTACTTTGAGAGAGAAGAAGATGAAGGAGTTAGTGGAATTTCTGGAATCAAAACAAGGATCTGTTGTGGAGATTAAAGATTTTGTTTTTACCTCTGTTTTCAAATCTCTTACTAACTTGATACAAAATCAAGTTAGAAAGCCAACAAACTTATTTCCAATTAAACTCACTTATATCTGA

Homology

[Close]

BLAST of CSPI05G08400 vs. Swiss-Prot
Match: C80B1_ESCCA ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Eschscholzia californica GN=CYP80B1 PE=2 SV=1)

HSP 1 Score: 136.7 bits (343), Expect = 2.9e-31
Identity = 68/159 (42.77%), Postives = 105/159 (66.04%), Query Frame = 1


Query: 38  LPPRPNPWPILGSLFHIGQNAHISITQFANIYGPLISLKLGAQRLIVASSRAATTAVLRT 97
           LPP P PWPI+G+L  +G+  H    + A  YG + +LK+G + ++VAS+ +A + +L+T
Sbjct: 28  LPPGPKPWPIVGNLLQLGEKPHAQFAELAQTYGDIFTLKMGTETVVVASTSSAASEILKT 87

Query: 98  QDCLLSARYIFQMTPDRALHDQHSLVFSPEYGDQWKNLRSICKVNLFTAKEIESQTTLRE 157
            D +LSARY+FQ    +  H ++S+V+S +  + WKNLR +C+  LFT K IESQ  +RE
Sbjct: 88  HDRILSARYVFQSFRVKG-HVENSIVWS-DCTETWKNLRKVCRTELFTQKMIESQAHVRE 147

Query: 158 KKMKELVEFLESKQGSVVEIKDFVFTSVFKSLTNLIQNQ 197
           KK +E+VE+L  KQG  V+I + +F ++     NLI +Q
Sbjct: 148 KKCEEMVEYLMKKQGEEVKIVEVIFGTLVNIFGNLIFSQ 184

[Close]

BLAST of CSPI05G08400 vs. Swiss-Prot
Match: C80B2_ESCCA ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Eschscholzia californica GN=CYP80B2 PE=2 SV=1)

HSP 1 Score: 131.7 bits (330), Expect = 9.3e-30
Identity = 73/189 (38.62%), Postives = 119/189 (62.96%), Query Frame = 1


Query: 8   ILLPSLLLLLLPSVFIILKHIKSPSSKKLPLPPRPNPWPILGSLFHIGQNAHISITQFAN 67
           ++  +L+ +++ S+  +L      SS    LPP P PWPI+G+L  +G+  H    + A 
Sbjct: 3   VVTVALIAVIISSILYLL----FGSSGHKNLPPGPKPWPIVGNLLQLGEKPHAQFAELAQ 62

Query: 68  IYGPLISLKLGAQRLIVASSRAATTAVLRTQDCLLSARYIFQMTPDRALHDQHSLVFSPE 127
            YG + +LK+G + ++VAS+ +A + +L+T D +LSARY+FQ    +  H ++S+V+S +
Sbjct: 63  TYGDIFTLKMGTETVVVASTSSAASEILKTHDRILSARYVFQSFRVKG-HVENSIVWS-D 122

Query: 128 YGDQWKNLRSICKVNLFTAKEIESQTTLREKKMKELVEFLESKQGSVVEIKDFVFTSVFK 187
             + WKNLR +C+  LFT K IESQ  +REKK +E+VE+L  KQG  V+I + +F ++  
Sbjct: 123 CTETWKNLRKVCRTELFTQKMIESQAHVREKKCEEMVEYLMKKQGEEVKIVEVIFGTLVN 182

Query: 188 SLTNLIQNQ 197
              NLI +Q
Sbjct: 183 IFGNLIFSQ 185

[Close]

BLAST of CSPI05G08400 vs. Swiss-Prot
Match: C80B3_PAPSO ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Papaver somniferum GN=CYP80B3 PE=1 SV=1)

HSP 1 Score: 129.4 bits (324), Expect = 4.6e-29
Identity = 73/185 (39.46%), Postives = 112/185 (60.54%), Query Frame = 1


Query: 12  SLLLLLLPSVFIILKHIKSPSSKKLPLPPRPNPWPILGSLFHIGQNAHISITQFANIYGP 71
           SL+ +++ +   ++    SP      LPP P PWPI+G+L  +G+  H    Q A  YG 
Sbjct: 1   SLVAVVITTFLYLIFRDSSPKG----LPPGPKPWPIVGNLLQLGEKPHSQFAQLAETYGD 60

Query: 72  LISLKLGAQRLIVASSRAATTAVLRTQDCLLSARYIFQMTPDRALHDQHSLVFSPEYGDQ 131
           L SLKLG++ ++VAS+  A + +L+T D +LS RY+FQ    +  H ++S+V+S E  + 
Sbjct: 61  LFSLKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKE-HVENSIVWS-ECNET 120

Query: 132 WKNLRSICKVNLFTAKEIESQTTLREKKMKELVEFLESKQGSVVEIKDFVFTSVFKSLTN 191
           WK LR +C+  LFT K IESQ  +RE K  E+VE+L+   G+ V+I + VF ++     N
Sbjct: 121 WKKLRKVCRTELFTQKMIESQAEVRESKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGN 179

Query: 192 LIQNQ 197
           LI +Q
Sbjct: 181 LIFSQ 179

[Close]

BLAST of CSPI05G08400 vs. Swiss-Prot
Match: C80B2_COPJA (Probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Coptis japonica GN=CYP80B2 PE=2 SV=1)

HSP 1 Score: 129.0 bits (323), Expect = 6.0e-29
Identity = 83/193 (43.01%), Postives = 116/193 (60.10%), Query Frame = 1


Query: 1   MLIPSFSILLPSLLLLLLPSVFIILKHIKSPSSKKLPLPPRPNPWPILGSLFHIGQNAHI 60
           M + S +I+  S LL L    F IL+      S+   LPP P P PI+G+L  +G   H 
Sbjct: 1   MEVLSIAIVSFSFLLFL----FFILR-----DSRPKNLPPGPRPSPIVGNLLQLGDKPHA 60

Query: 61  SITQFANIYGPLISLKLGAQRLIVASSRAATTAVLRTQDCLLSARYIFQMTPDRALHDQH 120
              + A  YG L SLKLG+Q ++VASS AA   +L+T D +LS RY+FQ    +  H ++
Sbjct: 61  EFAKLAQKYGELFSLKLGSQTVVVASSPAAAAEILKTHDKILSGRYVFQSFRVKE-HVEN 120

Query: 121 SLVFSPEYGDQWKNLRSICKVNLFTAKEIESQTTLREKKMKELVEFLESKQGSVVEIKDF 180
           S+V+S E  D WK LR +C+  LFT K IESQ+ +RE K +E+V+FL  K+G VV+I + 
Sbjct: 121 SIVWS-ECNDNWKLLRKVCRTELFTPKMIESQSEIREAKAREMVKFLRGKEGEVVKIVEV 180

Query: 181 VFTSVFKSLTNLI 194
           VF ++     NLI
Sbjct: 181 VFGTLVNIFGNLI 182

[Close]

BLAST of CSPI05G08400 vs. Swiss-Prot
Match: C80BX_PAPSO ((S)-N-methylcoclaurine 3'-hydroxylase-like protein OS=Papaver somniferum GN=CYP80BX PE=2 SV=1)

HSP 1 Score: 117.9 bits (294), Expect = 1.4e-25
Identity = 68/189 (35.98%), Postives = 110/189 (58.20%), Query Frame = 1


Query: 8   ILLPSLLLLLLPSVFIILKHIKSPSSKKLPLPPRPNPWPILGSLFHIGQNAHISITQFAN 67
           I+  +L+ ++  +   ++    SP+     LPP P PWPI+G+L  +G+  H    Q A 
Sbjct: 3   IVTVALIAIVFTTFLYLIVRESSPNG----LPPGPKPWPIVGNLLQLGEKPHSQFAQLAE 62

Query: 68  IYGPLISLKLGAQRLIVASSRAATTAVLRTQDCLLSARYIFQMTPDRALHDQHSLVFSPE 127
            YG L +LKLG Q ++VAS+  A + VL+  D  L  RY+FQ    +  H ++S+V++ E
Sbjct: 63  TYGDLFTLKLGTQTVVVASTPLAASEVLKAHDRTLCGRYVFQSFRVKN-HVENSIVWN-E 122

Query: 128 YGDQWKNLRSICKVNLFTAKEIESQTTLREKKMKELVEFLESKQGSVVEIKDFVFTSVFK 187
             + WK LR +C+  LFT K IE+Q  +RE K  E+V++L+  +G  V+I + +F ++  
Sbjct: 123 CNETWKKLRKVCRTQLFTQKMIENQAEVREIKTMEMVKYLKKNEGIEVKIVEVIFGTLVN 182

Query: 188 SLTNLIQNQ 197
              NLI +Q
Sbjct: 183 MFGNLIFSQ 185

[Close]

BLAST of CSPI05G08400 vs. TrEMBL
Match: A0A0A0KL66_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G173470 PE=4 SV=1)

HSP 1 Score: 384.0 bits (985), Expect = 1.2e-103
Identity = 200/211 (94.79%), Postives = 205/211 (97.16%), Query Frame = 1


Query: 1   MLIPSFSILLPSLLLLLLPSVFIILKHIKSPSSKKLPLPPRPNPWPILGSLFHIGQNAHI 60
           MLIPSFSILLPSLLLLLLPSVFIILKHIKSPSSKKLPLPP P+PWPI G+L  +GQNAH+
Sbjct: 1   MLIPSFSILLPSLLLLLLPSVFIILKHIKSPSSKKLPLPPGPSPWPIFGNLLQMGQNAHV 60

Query: 61  SITQFANIYGPLISLKLGAQRLIVASSRAATTAVLRTQDCLLSARYIFQMTPDRALHDQH 120
           SITQFAN+YGPLISLKLGAQRLIVASSRAATTAVLRTQDCLLSARYIFQMTPDRALHDQH
Sbjct: 61  SITQFANVYGPLISLKLGAQRLIVASSRAATTAVLRTQDCLLSARYIFQMTPDRALHDQH 120

Query: 121 SLVFSPEYGDQWKNLRSICKVNLFTAKEIESQTTLREKKMKELVEFLESKQGSVVEIKDF 180
           SLVFS EYGDQWKNLRSICKVNLFTAKEIESQ TLREKKMKELVEFLESKQGSVVEIKDF
Sbjct: 121 SLVFSHEYGDQWKNLRSICKVNLFTAKEIESQDTLREKKMKELVEFLESKQGSVVEIKDF 180

Query: 181 VFTSVFKSLTNLIQNQVRKPTNLFPIKLTYI 212
           VFTSVFKSLTNLIQNQVRKPTNLFPIKLTYI
Sbjct: 181 VFTSVFKSLTNLIQNQVRKPTNLFPIKLTYI 211

[Close]

BLAST of CSPI05G08400 vs. TrEMBL
Match: A0A0A0KPU9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G173490 PE=4 SV=1)

HSP 1 Score: 305.4 bits (781), Expect = 5.2e-80
Identity = 165/193 (85.49%), Postives = 172/193 (89.12%), Query Frame = 1


Query: 1   MLIPSFSILLPSLLLLLLPSVFIILKHIKSPSSKKLPLPPRPNPWPILGSLFHIGQNAHI 60
           MLIPSFSILLPSLLLLL P +FIILK IKSPSSKKLPLPP PNPWPILG+L  IGQNAHI
Sbjct: 11  MLIPSFSILLPSLLLLL-PPIFIILKRIKSPSSKKLPLPPGPNPWPILGNLLQIGQNAHI 70

Query: 61  SITQFANIYGPLISLKLGAQRLIVASSRAATTAVLRTQDCLLSARYIFQMTPDRALHDQH 120
           SITQFANIYGPLIS KLGAQRL+VASS AA TAVL+TQD LLSARYIFQ+TPDRALHDQH
Sbjct: 71  SITQFANIYGPLISFKLGAQRLVVASSPAAATAVLKTQDHLLSARYIFQITPDRALHDQH 130

Query: 121 SLVFSPEYGDQWKNLRSICKVNLFTAKEIESQTTLREKKMKELVEFLESKQGSVVEIKDF 180
           SLVFS E GD WK LRSICKVNLFTAK  ESQ  LREKKMKELVEF+ESKQGSVVEIKDF
Sbjct: 131 SLVFSSECGDVWKKLRSICKVNLFTAKAFESQAILREKKMKELVEFVESKQGSVVEIKDF 190

Query: 181 VFTSVFKSLTNLI 194
           VFTS F  L+NLI
Sbjct: 191 VFTSAFNILSNLI 202

[Close]

BLAST of CSPI05G08400 vs. TrEMBL
Match: A0A0A0KRT5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G173480 PE=4 SV=1)

HSP 1 Score: 298.5 bits (763), Expect = 6.4e-78
Identity = 161/193 (83.42%), Postives = 171/193 (88.60%), Query Frame = 1


Query: 1   MLIPSFSILLPSLLLLLLPSVFIILKHIKSPSSKKLPLPPRPNPWPILGSLFHIGQNAHI 60
           MLIPSFSILLPSLLLL+ P +FIILK IKSPS KKLPLPP PNPWPILG+L  IGQNAHI
Sbjct: 1   MLIPSFSILLPSLLLLI-PPIFIILKRIKSPSLKKLPLPPGPNPWPILGNLPQIGQNAHI 60

Query: 61  SITQFANIYGPLISLKLGAQRLIVASSRAATTAVLRTQDCLLSARYIFQMTPDRALHDQH 120
           SITQFANIYGPLISLKLGAQRL+VASS A  TAVL+T D  LS RYI QMTPDRAL++QH
Sbjct: 61  SITQFANIYGPLISLKLGAQRLVVASSPATATAVLKTHDRFLSNRYILQMTPDRALYNQH 120

Query: 121 SLVFSPEYGDQWKNLRSICKVNLFTAKEIESQTTLREKKMKELVEFLESKQGSVVEIKDF 180
           SLVFSPE GD WKNLRSICK NLFTAK IESQ  LREKKMKELVEFLESKQGSVV+IKDF
Sbjct: 121 SLVFSPECGDHWKNLRSICKANLFTAKAIESQAILREKKMKELVEFLESKQGSVVKIKDF 180

Query: 181 VFTSVFKSLTNLI 194
           VFTSVF +L+NLI
Sbjct: 181 VFTSVFNTLSNLI 192

[Close]

BLAST of CSPI05G08400 vs. TrEMBL
Match: B9RHY2_RICCO ((S)-N-methylcoclaurine 3'-hydroxylase isozyme, putative OS=Ricinus communis GN=RCOM_1574460 PE=4 SV=1)

HSP 1 Score: 179.1 bits (453), Expect = 5.7e-42
Identity = 101/195 (51.79%), Postives = 141/195 (72.31%), Query Frame = 1


Query: 2   LIPSFSILLPSLLLLLLPSVFIILKHIKSPSSKKLP-LPPRPNPWPILGSLFHIGQNAHI 61
           L+  F+     LLLLLLP +FIILKHI +  S K P LPP P PWPI+G+L  IG+  HI
Sbjct: 7   LLNQFASSFYLLLLLLLPLIFIILKHIVTSLSSKRPRLPPGPRPWPIIGNLHQIGKKLHI 66

Query: 62  SITQFANIYGPLISLKLGAQRLIVASSRAATTAVLRTQDCLLSARYIFQMTPDRALH--D 121
           S+TQFA ++GPLISL+LG++ ++V SS  A T +L+T D LLSAR I ++ P + +H  D
Sbjct: 67  SVTQFAKVHGPLISLRLGSRVVVVGSSPLAATEILKTHDRLLSARTIPKVLPYK-IHFVD 126

Query: 122 QHSLVFSPEYGDQWKNLRSICKVNLFTAKEIESQTTLREKKMKELVEFLESKQGSVVEIK 181
           + ++V++    ++WK+LR++C+  LF+A  IESQ  LREKKM E+VE+L S+QG VV I 
Sbjct: 127 RLAIVWASSCNERWKSLRALCRTELFSANAIESQAALREKKMIEMVEYLSSEQGQVVNIG 186

Query: 182 DFVFTSVFKSLTNLI 194
           + VFTSVF +++NLI
Sbjct: 187 EVVFTSVFNTISNLI 200

[Close]

BLAST of CSPI05G08400 vs. TrEMBL
Match: B9RHX8_RICCO ((S)-N-methylcoclaurine 3'-hydroxylase isozyme, putative OS=Ricinus communis GN=RCOM_1574420 PE=3 SV=1)

HSP 1 Score: 173.7 bits (439), Expect = 2.4e-40
Identity = 94/191 (49.21%), Postives = 136/191 (71.20%), Query Frame = 1


Query: 5   SFSILLPSLLLLLLPSVFIILKHIKSPSSKKLPLPPRPNPWPILGSLFHIGQNAHISITQ 64
           +F++  PS LLL L  + I+++ + S SSK+ PLPP P  WPILG++  +G+N HIS+  
Sbjct: 5   AFTLTSPSFLLLPL-ILIIVMQILSSISSKRRPLPPGPRQWPILGNILQVGKNPHISMAN 64

Query: 65  FANIYGPLISLKLGAQRLIVASSRAATTAVLRTQDCLLSARYIFQMTP--DRALHDQHSL 124
           +A ++GPLISL+LG + ++VASS  A   +L+T D LLS RYI   TP  D  L D+ +L
Sbjct: 65  YAKVHGPLISLRLGTRVVVVASSPTAAAEILKTHDRLLSGRYIPATTPYEDNVL-DRIAL 124

Query: 125 VFSPEYGDQWKNLRSICKVNLFTAKEIESQTTLREKKMKELVEFLESKQGSVVEIKDFVF 184
           V++P   DQWK LR++C+  LF+AK IESQ TLREKK+ E+++FL SKQG +V I + VF
Sbjct: 125 VWNPSCSDQWKFLRAMCRSELFSAKAIESQATLREKKLTEMLDFLTSKQGQIVNIGEVVF 184

Query: 185 TSVFKSLTNLI 194
           T+ F +++NL+
Sbjct: 185 TTAFNTISNLL 193

[Close]

BLAST of CSPI05G08400 vs. TAIR10
Match: AT2G45570.1 (AT2G45570.1 cytochrome P450, family 76, subfamily C, polypeptide 2)

HSP 1 Score: 100.9 bits (250), Expect = 9.9e-22
Identity = 66/188 (35.11%), Postives = 102/188 (54.26%), Query Frame = 1


Query: 14  LLLLLPSVFIILKHIKSPSS--KKLPLPPRPNPWPILGSLFHIGQNAHISITQFANIYGP 73
           L   + S FII      P S  K +P PP P   PI+G++  +G+N H S    +  YGP
Sbjct: 12  LFCFVLSFFIIFFTTTRPRSSRKVVPSPPGPPRLPIIGNIHLVGRNPHHSFADLSKTYGP 71

Query: 74  LISLKLGAQRLIVASSRAATTAVLRTQDCLLSARYIFQMTPDRAL----HDQHSLVFSPE 133
           ++SLK G+   +V +S  A   VLRT D +LS+R     TP  ++    HD+ S+V+ P 
Sbjct: 72  IMSLKFGSLNTVVVTSPEAAREVLRTYDQILSSR-----TPTNSIRSINHDKVSVVWLPP 131

Query: 134 YGDQWKNLRSICKVNLFTAKEIESQTTLREKKMKELVEFL--ESKQGSVVEIKDFVFTSV 193
              +W+ LR +    LF+ + IE+  TLRE K+KELV F+   S++   V+I    F + 
Sbjct: 132 SSSRWRLLRKLSATQLFSPQRIEATKTLRENKVKELVSFMSESSEREEAVDISRATFITA 191

[Close]

BLAST of CSPI05G08400 vs. TAIR10
Match: AT2G45550.1 (AT2G45550.1 cytochrome P450, family 76, subfamily C, polypeptide 4)

HSP 1 Score: 90.9 bits (224), Expect = 1.0e-18
Identity = 61/186 (32.80%), Postives = 99/186 (53.23%), Query Frame = 1


Query: 13  LLLLLLPSVFIILKHIKS--PSSKKLPLPPRPNPWPILGSLFHIGQNAHISITQFANIYG 72
           LL   + S F+I    +S   S +   LPP P   PI+G++  +G+N H S    A IYG
Sbjct: 11  LLFCFISSCFLISTTARSRRSSGRAATLPPGPPRLPIIGNIHQVGKNPHSSFADLAKIYG 70

Query: 73  PLISLKLGAQRLIVASSRAATTAVLRTQDCLLSARYIFQMTPDRAL-HDQHSLVFSPEYG 132
           P++SLK G    +V +S  A   VLRT D +LS R        R   H++ S+++ P   
Sbjct: 71  PIMSLKFGCLNSVVITSPEAAREVLRTHDQILSGRK--SNDSIRCFGHEEVSVIWLPPSS 130

Query: 133 DQWKNLRSICKVNLFTAKEIESQTTLREKKMKELVEFL--ESKQGSVVEIKDFVFTSVFK 192
            +W+ LR +    +F+ +  E+   LR KK++ELV F+   S++   V+I    +T+V  
Sbjct: 131 ARWRMLRKLSVTLMFSPQRTEATKALRMKKVQELVSFMNESSERKEAVDISRASYTTVLN 190

Query: 193 SLTNLI 194
            ++N++
Sbjct: 191 IISNIL 194

[Close]

BLAST of CSPI05G08400 vs. TAIR10
Match: AT1G33720.1 (AT1G33720.1 cytochrome P450, family 76, subfamily C, polypeptide 6)

HSP 1 Score: 89.7 bits (221), Expect = 2.3e-18
Identity = 53/157 (33.76%), Postives = 87/157 (55.41%), Query Frame = 1


Query: 39  PPRPNPWPILGSLFHIGQNAHISITQFANIYGPLISLKLGAQRLIVASSRAATTAVLRTQ 98
           PP P   PI+G++  +G+N H S T  +  YGP++SLKLG    +V +SR A   VL+T 
Sbjct: 39  PPGPPRLPIIGNIHLVGKNPHHSFTDLSKTYGPVMSLKLGCLNSVVIASRDAVREVLKTH 98

Query: 99  DCLLSARYIFQMTPDRALHDQHSLVFSPEYGDQWKNLRSICKVNLFTAKEIESQTTLREK 158
           D +LS RYI + T     H + S+ +      +++ LR +    LF+ + I++   LR K
Sbjct: 99  DQILSGRYISEATKSNN-HHEFSVGWIHPSSSRFRMLRKLSATQLFSPQCIQATKALRMK 158

Query: 159 KMKELVEFLES--KQGSVVEIKDFVFTSVFKSLTNLI 194
           K++ELV FL    ++   V+I    F +    ++N++
Sbjct: 159 KVQELVNFLSESCEREEAVDISHVSFVTALNIISNIL 194

[Close]

BLAST of CSPI05G08400 vs. TAIR10
Match: AT2G45560.1 (AT2G45560.1 cytochrome P450, family 76, subfamily C, polypeptide 1)

HSP 1 Score: 87.8 bits (216), Expect = 8.7e-18
Identity = 63/189 (33.33%), Postives = 98/189 (51.85%), Query Frame = 1


Query: 13  LLLLLLPSVFIILKHIKSP--SSKKLPLPPRPNPWPILGSLFHIGQNAHISITQFANIYG 72
           LL   + S F+I    +S   S     LPP P   PI+G++  +G++ H S  + +  YG
Sbjct: 11  LLFCFILSCFLIFTTTRSGRISRGATALPPGPPRLPIIGNIHLVGKHPHRSFAELSKTYG 70

Query: 73  PLISLKLGAQRLIVASSRAATTAVLRTQDCLLSARYIFQMTPDRAL----HDQHSLVFSP 132
           P++SLKLG+   +V +S  A   VLRT D +LSAR     +P  A+    H   SLV+ P
Sbjct: 71  PVMSLKLGSLNTVVIASPEAAREVLRTHDQILSAR-----SPTNAVRSINHQDASLVWLP 130

Query: 133 EYGDQWKNLRSICKVNLFTAKEIESQTTLREKKMKELVEFL--ESKQGSVVEIKDFVFTS 192
               +W+ LR +    L + + IE+   LR  K+KELV F+   S +   V+I    F +
Sbjct: 131 SSSARWRLLRRLSVTQLLSPQRIEATKALRMNKVKELVSFISESSDREESVDISRVAFIT 190

Query: 193 VFKSLTNLI 194
               ++N++
Sbjct: 191 TLNIISNIL 194

[Close]

BLAST of CSPI05G08400 vs. TAIR10
Match: AT5G07990.1 (AT5G07990.1 Cytochrome P450 superfamily protein)

HSP 1 Score: 83.2 bits (204), Expect = 2.1e-16
Identity = 55/161 (34.16%), Postives = 91/161 (56.52%), Query Frame = 1


Query: 7   SILLPSLLLLLLPSVFIILKHIKSPSSKKLPLPPRPNPWPILGSLFHIGQNAHISITQFA 66
           +ILL ++L L+L     I  H ++ S     LPP PNPWPI+G+L H+G   H +++   
Sbjct: 7   TILLATVLFLILR----IFSHRRNRSHNNR-LPPGPNPWPIIGNLPHMGTKPHRTLSAMV 66

Query: 67  NIYGPLISLKLGAQRLIVASSRAATTAVLRTQDCLLSARYIFQMTPDRALHDQHSLVFSP 126
             YGP++ L+LG   ++VA+S++     L+  D   ++R         A ++   LVF+P
Sbjct: 67  TTYGPILHLRLGFVDVVVAASKSVAEQFLKIHDANFASRPPNSGAKHMA-YNYQDLVFAP 126

Query: 127 EYGDQWKNLRSICKVNLFTAKEIESQTTLREKKMKELVEFL 168
            YG +W+ LR I  V+LF+AK +E    +R++++  L   L
Sbjct: 127 -YGHRWRLLRKISSVHLFSAKALEDFKHVRQEEVGTLTREL 160

[Close]

BLAST of CSPI05G08400 vs. NCBI nr
Match: gi|700195243|gb|KGN50420.1| (hypothetical protein Csa_5G173470 [Cucumis sativus])

HSP 1 Score: 384.0 bits (985), Expect = 1.7e-103
Identity = 200/211 (94.79%), Postives = 205/211 (97.16%), Query Frame = 1


Query: 1   MLIPSFSILLPSLLLLLLPSVFIILKHIKSPSSKKLPLPPRPNPWPILGSLFHIGQNAHI 60
           MLIPSFSILLPSLLLLLLPSVFIILKHIKSPSSKKLPLPP P+PWPI G+L  +GQNAH+
Sbjct: 1   MLIPSFSILLPSLLLLLLPSVFIILKHIKSPSSKKLPLPPGPSPWPIFGNLLQMGQNAHV 60

Query: 61  SITQFANIYGPLISLKLGAQRLIVASSRAATTAVLRTQDCLLSARYIFQMTPDRALHDQH 120
           SITQFAN+YGPLISLKLGAQRLIVASSRAATTAVLRTQDCLLSARYIFQMTPDRALHDQH
Sbjct: 61  SITQFANVYGPLISLKLGAQRLIVASSRAATTAVLRTQDCLLSARYIFQMTPDRALHDQH 120

Query: 121 SLVFSPEYGDQWKNLRSICKVNLFTAKEIESQTTLREKKMKELVEFLESKQGSVVEIKDF 180
           SLVFS EYGDQWKNLRSICKVNLFTAKEIESQ TLREKKMKELVEFLESKQGSVVEIKDF
Sbjct: 121 SLVFSHEYGDQWKNLRSICKVNLFTAKEIESQDTLREKKMKELVEFLESKQGSVVEIKDF 180

Query: 181 VFTSVFKSLTNLIQNQVRKPTNLFPIKLTYI 212
           VFTSVFKSLTNLIQNQVRKPTNLFPIKLTYI
Sbjct: 181 VFTSVFKSLTNLIQNQVRKPTNLFPIKLTYI 211

[Close]

BLAST of CSPI05G08400 vs. NCBI nr
Match: gi|778708322|ref|XP_011656165.1| (PREDICTED: (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2-like [Cucumis sativus])

HSP 1 Score: 354.0 bits (907), Expect = 1.8e-94
Identity = 185/196 (94.39%), Postives = 190/196 (96.94%), Query Frame = 1


Query: 1   MLIPSFSILLPSLLLLLLPSVFIILKHIKSPSSKKLPLPPRPNPWPILGSLFHIGQNAHI 60
           MLIPSFSILLPSLLLLLLPSVFIILKHIKSPSSKKLPLPP P+PWPI G+L  +GQNAH+
Sbjct: 1   MLIPSFSILLPSLLLLLLPSVFIILKHIKSPSSKKLPLPPGPSPWPIFGNLLQMGQNAHV 60

Query: 61  SITQFANIYGPLISLKLGAQRLIVASSRAATTAVLRTQDCLLSARYIFQMTPDRALHDQH 120
           SITQFAN+YGPLISLKLGAQRLIVASSRAATTAVLRTQDCLLSARYIFQMTPDRALHDQH
Sbjct: 61  SITQFANVYGPLISLKLGAQRLIVASSRAATTAVLRTQDCLLSARYIFQMTPDRALHDQH 120

Query: 121 SLVFSPEYGDQWKNLRSICKVNLFTAKEIESQTTLREKKMKELVEFLESKQGSVVEIKDF 180
           SLVFS EYGDQWKNLRSICKVNLFTAKEIESQ TLREKKMKELVEFLESKQGSVVEIKDF
Sbjct: 121 SLVFSHEYGDQWKNLRSICKVNLFTAKEIESQDTLREKKMKELVEFLESKQGSVVEIKDF 180

Query: 181 VFTSVFKSLTNLIQNQ 197
           VFTSVFKSLTNLIQNQ
Sbjct: 181 VFTSVFKSLTNLIQNQ 196

[Close]

BLAST of CSPI05G08400 vs. NCBI nr
Match: gi|659073029|ref|XP_008467223.1| (PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucumis melo])

HSP 1 Score: 313.2 bits (801), Expect = 3.6e-82
Identity = 170/193 (88.08%), Postives = 178/193 (92.23%), Query Frame = 1


Query: 1   MLIPSFSILLPSLLLLLLPSVFIILKHIKSPSSKKLPLPPRPNPWPILGSLFHIGQNAHI 60
           MLIPSFSILLPSLLLLL   +FIILKHIKSPSSK LPLPP PNPWPILG+L  IGQNAHI
Sbjct: 1   MLIPSFSILLPSLLLLL--PIFIILKHIKSPSSK-LPLPPGPNPWPILGNLLQIGQNAHI 60

Query: 61  SITQFANIYGPLISLKLGAQRLIVASSRAATTAVLRTQDCLLSARYIFQMTPDRALHDQH 120
           SITQFANIYGPLISLKLGAQRLIVASS AA TAVL+TQD LLSARYIFQMTPDRALHDQH
Sbjct: 61  SITQFANIYGPLISLKLGAQRLIVASSPAAATAVLKTQDRLLSARYIFQMTPDRALHDQH 120

Query: 121 SLVFSPEYGDQWKNLRSICKVNLFTAKEIESQTTLREKKMKELVEFLESKQGSVVEIKDF 180
           SLVFSPE GDQWKNLRS+CKVNLFTAK IESQ  LREKKMKELV+FLESKQGSVVE+KDF
Sbjct: 121 SLVFSPECGDQWKNLRSLCKVNLFTAKAIESQAILREKKMKELVKFLESKQGSVVEVKDF 180

Query: 181 VFTSVFKSLTNLI 194
           VFTSVF +L+NLI
Sbjct: 181 VFTSVFNTLSNLI 190

[Close]

BLAST of CSPI05G08400 vs. NCBI nr
Match: gi|700195245|gb|KGN50422.1| (hypothetical protein Csa_5G173490 [Cucumis sativus])

HSP 1 Score: 305.4 bits (781), Expect = 7.5e-80
Identity = 165/193 (85.49%), Postives = 172/193 (89.12%), Query Frame = 1


Query: 1   MLIPSFSILLPSLLLLLLPSVFIILKHIKSPSSKKLPLPPRPNPWPILGSLFHIGQNAHI 60
           MLIPSFSILLPSLLLLL P +FIILK IKSPSSKKLPLPP PNPWPILG+L  IGQNAHI
Sbjct: 11  MLIPSFSILLPSLLLLL-PPIFIILKRIKSPSSKKLPLPPGPNPWPILGNLLQIGQNAHI 70

Query: 61  SITQFANIYGPLISLKLGAQRLIVASSRAATTAVLRTQDCLLSARYIFQMTPDRALHDQH 120
           SITQFANIYGPLIS KLGAQRL+VASS AA TAVL+TQD LLSARYIFQ+TPDRALHDQH
Sbjct: 71  SITQFANIYGPLISFKLGAQRLVVASSPAAATAVLKTQDHLLSARYIFQITPDRALHDQH 130

Query: 121 SLVFSPEYGDQWKNLRSICKVNLFTAKEIESQTTLREKKMKELVEFLESKQGSVVEIKDF 180
           SLVFS E GD WK LRSICKVNLFTAK  ESQ  LREKKMKELVEF+ESKQGSVVEIKDF
Sbjct: 131 SLVFSSECGDVWKKLRSICKVNLFTAKAFESQAILREKKMKELVEFVESKQGSVVEIKDF 190

Query: 181 VFTSVFKSLTNLI 194
           VFTS F  L+NLI
Sbjct: 191 VFTSAFNILSNLI 202

[Close]

BLAST of CSPI05G08400 vs. NCBI nr
Match: gi|700195244|gb|KGN50421.1| (hypothetical protein Csa_5G173480 [Cucumis sativus])

HSP 1 Score: 298.5 bits (763), Expect = 9.2e-78
Identity = 161/193 (83.42%), Postives = 171/193 (88.60%), Query Frame = 1


Query: 1   MLIPSFSILLPSLLLLLLPSVFIILKHIKSPSSKKLPLPPRPNPWPILGSLFHIGQNAHI 60
           MLIPSFSILLPSLLLL+ P +FIILK IKSPS KKLPLPP PNPWPILG+L  IGQNAHI
Sbjct: 1   MLIPSFSILLPSLLLLI-PPIFIILKRIKSPSLKKLPLPPGPNPWPILGNLPQIGQNAHI 60

Query: 61  SITQFANIYGPLISLKLGAQRLIVASSRAATTAVLRTQDCLLSARYIFQMTPDRALHDQH 120
           SITQFANIYGPLISLKLGAQRL+VASS A  TAVL+T D  LS RYI QMTPDRAL++QH
Sbjct: 61  SITQFANIYGPLISLKLGAQRLVVASSPATATAVLKTHDRFLSNRYILQMTPDRALYNQH 120

Query: 121 SLVFSPEYGDQWKNLRSICKVNLFTAKEIESQTTLREKKMKELVEFLESKQGSVVEIKDF 180
           SLVFSPE GD WKNLRSICK NLFTAK IESQ  LREKKMKELVEFLESKQGSVV+IKDF
Sbjct: 121 SLVFSPECGDHWKNLRSICKANLFTAKAIESQAILREKKMKELVEFLESKQGSVVKIKDF 180

Query: 181 VFTSVFKSLTNLI 194
           VFTSVF +L+NLI
Sbjct: 181 VFTSVFNTLSNLI 192

The following BLAST results are available for this feature:

BLAST of CSPI05G08400 vs. Swiss-Prot


Match Name	E-value	Identity	Description
C80B1_ESCCA	2.9e-31	42.77	(S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Eschscholzia calif...	[more]
C80B2_ESCCA	9.3e-30	38.62	(S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Eschscholzia californica GN=C...	[more]
C80B3_PAPSO	4.6e-29	39.46	(S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Papaver somniferum...	[more]
C80B2_COPJA	6.0e-29	43.01	Probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Coptis japonica GN=C...	[more]
C80BX_PAPSO	1.4e-25	35.98	(S)-N-methylcoclaurine 3'-hydroxylase-like protein OS=Papaver somniferum GN=CYP8...	[more]

BLAST of CSPI05G08400 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KL66_CUCSA	1.2e-103	94.79	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G173470 PE=4 SV=1	[more]
A0A0A0KPU9_CUCSA	5.2e-80	85.49	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G173490 PE=4 SV=1	[more]
A0A0A0KRT5_CUCSA	6.4e-78	83.42	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G173480 PE=4 SV=1	[more]
B9RHY2_RICCO	5.7e-42	51.79	(S)-N-methylcoclaurine 3'-hydroxylase isozyme, putative OS=Ricinus communis GN=R...	[more]
B9RHX8_RICCO	2.4e-40	49.21	(S)-N-methylcoclaurine 3'-hydroxylase isozyme, putative OS=Ricinus communis GN=R...	[more]

BLAST of CSPI05G08400 vs. TAIR10


Match Name	E-value	Identity	Description
AT2G45570.1	9.9e-22	35.11	cytochrome P450, family 76, subfamily C, polypeptide 2	[more]
AT2G45550.1	1.0e-18	32.80	cytochrome P450, family 76, subfamily C, polypeptide 4	[more]
AT1G33720.1	2.3e-18	33.76	cytochrome P450, family 76, subfamily C, polypeptide 6	[more]
AT2G45560.1	8.7e-18	33.33	cytochrome P450, family 76, subfamily C, polypeptide 1	[more]
AT5G07990.1	2.1e-16	34.16	Cytochrome P450 superfamily protein	[more]

BLAST of CSPI05G08400 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700195243\|gb\|KGN50420.1\|	1.7e-103	94.79	hypothetical protein Csa_5G173470 [Cucumis sativus]	[more]
gi\|778708322\|ref\|XP_011656165.1\|	1.8e-94	94.39	PREDICTED: (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2-like [Cucumis sativus...	[more]
gi\|659073029\|ref\|XP_008467223.1\|	3.6e-82	88.08	PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucumis mel...	[more]
gi\|700195245\|gb\|KGN50422.1\|	7.5e-80	85.49	hypothetical protein Csa_5G173490 [Cucumis sativus]	[more]
gi\|700195244\|gb\|KGN50421.1\|	9.2e-78	83.42	hypothetical protein Csa_5G173480 [Cucumis sativus]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR001128	Cyt_P450

Vocabulary: **Molecular Function**
Term	Definition
GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
GO:0005506	iron ion binding
GO:0020037	heme binding

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	oxidation-reduction process

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055114	oxidation-reduction process
cellular_component	GO:0005575	cellular_component
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
molecular_function	GO:0005515	protein binding
molecular_function	GO:0050593	N-methylcoclaurine 3'-monooxygenase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI05G08400.1	CSPI05G08400.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	GENE3D	G3DSA:1.10.630.10		coord: 33..193 score: 2.1
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 39..193 score: 3.3
IPR001128	Cytochrome P450	unknown	SSF48264	Cytochrome P450	coord: 34..193 score: 8.64
None	No IPR available	PANTHER	PTHR24298	FAMILY NOT NAMED	coord: 1..193 score: 6.7
None	No IPR available	PANTHER	PTHR24298:SF55	SUBFAMILY NOT NAMED	coord: 1..193 score: 6.7

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CSPI05G08400	Csa5G173470	Cucumber (Chinese Long) v2	cpicuB240
CSPI05G08400	MELO3C005375	Melon (DHL92) v3.5.1	cpimeB351
CSPI05G08400	Lsi09G005880	Bottle gourd (USVL1VR-Ls)	cpilsiB322
CSPI05G08400	MELO3C005375.2	Melon (DHL92) v3.6.1	cpimedB341
CSPI05G08400	CsaV3_5G005790	Cucumber (Chinese Long) v3	cpicucB285
CSPI05G08400	Cla97C01G005800	Watermelon (97103) v2	cpiwmbB349
CSPI05G08400	Bhi12G000482	Wax gourd	cpiwgoB458
CSPI05G08400	Cucsa.166420	Cucumber (Gy14) v1	cgycpiB258
CSPI05G08400	CmoCh15G014820	Cucurbita moschata (Rifu)	cmocpiB287
CSPI05G08400	Cp4.1LG13g00480	Cucurbita pepo (Zucchini)	cpecpiB187
CSPI05G08400	CsGy5G005790	Cucumber (Gy14) v2	cgybcpiB226
CSPI05G08400	Carg20770	Silver-seed gourd	carcpiB0773

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CSPI05G08400	Cucumber (Chinese Long) v3	cpicucB265
CSPI05G08400	Wild cucumber (PI 183967)	cpicpiB113
CSPI05G08400	Cucumber (Gy14) v1	cgycpiB388
CSPI05G08400	Cucurbita maxima (Rimu)	cmacpiB296
CSPI05G08400	Cucurbita maxima (Rimu)	cmacpiB615
CSPI05G08400	Cucurbita moschata (Rifu)	cmocpiB607
CSPI05G08400	Melon (DHL92) v3.5.1	cpimeB400
CSPI05G08400	Watermelon (Charleston Gray)	cpiwcgB379
CSPI05G08400	Watermelon (97103) v1	cpiwmB409
CSPI05G08400	Watermelon (97103) v1	cpiwmB419
CSPI05G08400	Cucurbita pepo (Zucchini)	cpecpiB739
CSPI05G08400	Bottle gourd (USVL1VR-Ls)	cpilsiB384
CSPI05G08400	Melon (DHL92) v3.6.1	cpimedB395
CSPI05G08400	Cucumber (Gy14) v2	cgybcpiB129
CSPI05G08400	Silver-seed gourd	carcpiB0749