CSPI05G06480 (gene) Wild cucumber (PI 183967)

NameCSPI05G06480
Typegene
OrganismCucumis sativus (Wild cucumber (PI 183967))
DescriptionPlant invertase/pectin methylesterase inhibitor
LocationChr5 : 5808266 .. 5809260 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTCCTAATTTTTTTGTTTTTGTTTTTGAAATCAAAACTAACCATCATCGTACCAATAATTACACTCTCCTTCTTCTTCCCTAAATATTCATTTCACAGATTCCCCTGTTTTGTGGGATCCCACTATCCTTTTATTACCAAATTCCCCCCCATAAATTTGTTTCCCCCCTTCTTCTCCAGATCTTCACATCCAGATCCAAATCAACAACAATGGAGAATCCCCTCCCCACACTCCTCCCCCTCCTCCTCCTCCTCATAATCTCCGATCAAACTCAAATCCTCTCCGTCGCCGCCTCAACACTCCCCCGCAAGTCCTCTGCCGGAATCAGAACTAATACAGAGTATGTCAGAACCTCCTGCAGTACTACTTCATACCCTCGTCTCTGCTACAATTCACTCTCGGTTTACGCCGGGAAGATCAAGACCAACCCCAAGACGCTGGCTCTCGCCGCCCTCCACGTTAACCTCGCCGCCGCCCGATCCTCAGCTGCATCAATGAGAAGGCTGGCGAAAACCCGCGGCCTGCGGCGGAGAGACGCATCGGCGATCTCTGATTGTGTGGAGGAGGTTGGAGATTCGGTTTTTGAGCTACAGAGAGCGATTCGGGAACTGGGACGGCCTCGAGGGTATGATTTTATGGGGTTGATCAGTGACATTGAGACGTGGGTTAGCTCTGCTCTGACGGACGAAGAGACGTGTATGGAAGGATTTGGGGGACGGCGTGTTAATGGAGTTAGTGTTAAGGCCAAAGTTCGAAGACACATTGTTAGAGTTGCGCATTTGACGAGTAATTCACTTGCTCTTATTAATAGCTATGCTTCGTCGGCCGCGGCGGAAGAAGGCGTCTTGCCCTAGGCATTTTTCTTTCTTTTTCTGTTTGGTTCTTGGGAAAAGTGAAAGGAAATTTGGAATGTTATGAGATTGGAAATGTAATTATTATTATGTAACTCTAGTGTTACGGTTTATGAAATTATTATTACTTAATTATGCTTCAAT

mRNA sequence

ATGGAGAATCCCCTCCCCACACTCCTCCCCCTCCTCCTCCTCCTCATAATCTCCGATCAAACTCAAATCCTCTCCGTCGCCGCCTCAACACTCCCCCGCAAGTCCTCTGCCGGAATCAGAACTAATACAGAGTATGTCAGAACCTCCTGCAGTACTACTTCATACCCTCGTCTCTGCTACAATTCACTCTCGGTTTACGCCGGGAAGATCAAGACCAACCCCAAGACGCTGGCTCTCGCCGCCCTCCACGTTAACCTCGCCGCCGCCCGATCCTCAGCTGCATCAATGAGAAGGCTGGCGAAAACCCGCGGCCTGCGGCGGAGAGACGCATCGGCGATCTCTGATTGTGTGGAGGAGGTTGGAGATTCGGTTTTTGAGCTACAGAGAGCGATTCGGGAACTGGGACGGCCTCGAGGGTATGATTTTATGGGGTTGATCAGTGACATTGAGACGTGGGTTAGCTCTGCTCTGACGGACGAAGAGACGTGTATGGAAGGATTTGGGGGACGGCGTGTTAATGGAGTTAGTGTTAAGGCCAAAGTTCGAAGACACATTGTTAGAGTTGCGCATTTGACGAGTAATTCACTTGCTCTTATTAATAGCTATGCTTCGTCGGCCGCGGCGGAAGAAGGCGTCTTGCCCTAG

Coding sequence (CDS)

ATGGAGAATCCCCTCCCCACACTCCTCCCCCTCCTCCTCCTCCTCATAATCTCCGATCAAACTCAAATCCTCTCCGTCGCCGCCTCAACACTCCCCCGCAAGTCCTCTGCCGGAATCAGAACTAATACAGAGTATGTCAGAACCTCCTGCAGTACTACTTCATACCCTCGTCTCTGCTACAATTCACTCTCGGTTTACGCCGGGAAGATCAAGACCAACCCCAAGACGCTGGCTCTCGCCGCCCTCCACGTTAACCTCGCCGCCGCCCGATCCTCAGCTGCATCAATGAGAAGGCTGGCGAAAACCCGCGGCCTGCGGCGGAGAGACGCATCGGCGATCTCTGATTGTGTGGAGGAGGTTGGAGATTCGGTTTTTGAGCTACAGAGAGCGATTCGGGAACTGGGACGGCCTCGAGGGTATGATTTTATGGGGTTGATCAGTGACATTGAGACGTGGGTTAGCTCTGCTCTGACGGACGAAGAGACGTGTATGGAAGGATTTGGGGGACGGCGTGTTAATGGAGTTAGTGTTAAGGCCAAAGTTCGAAGACACATTGTTAGAGTTGCGCATTTGACGAGTAATTCACTTGCTCTTATTAATAGCTATGCTTCGTCGGCCGCGGCGGAAGAAGGCGTCTTGCCCTAG
BLAST of CSPI05G06480 vs. Swiss-Prot
Match: 21KD_DAUCA (21 kDa protein OS=Daucus carota PE=2 SV=1)

HSP 1 Score: 144.1 bits (362), Expect = 1.8e-33
Identity = 80/191 (41.88%), Postives = 122/191 (63.87%), Query Frame = 1

Query: 17  ISDQTQILSVAASTLPRKSSAGIRTNTEYVRTSCSTTSYPRLCYNSLSVYAGKIKTNPKT 76
           +S  T + S     L   S+A      ++++TSC+ T+YP +C  SLS YA  I+ NP+ 
Sbjct: 3   LSKSTLVFSALLVILAAASAAPAN---QFIKTSCTLTTYPAVCEQSLSAYAKTIQNNPQE 62

Query: 77  LALAALHVNLAAARSSAASMRRLAKTRGLRRRDASAISDCVEEVGDSVFELQRA---IRE 136
           LA  AL V+L   + +   M+RL K +GL+ R  +AI DC+EEV DS+  + R+   ++ 
Sbjct: 63  LASTALQVSLTRTQQAQTFMKRLNKFKGLKARQYAAIHDCLEEVEDSLDRVSRSCDEMKN 122

Query: 137 LGRPRGYDFMGLISDIETWVSSALTDEETCMEGFGGRRVNGVSVKAKVRRHIVRVAHLTS 196
           L   +G DF   +S++ETWVS+ALTDE TCM+GF G+ ++G  +K  VR  +V VA +TS
Sbjct: 123 LSHAKGNDFTFRMSNVETWVSAALTDETTCMDGFAGKGMDG-KIKESVRAQVVAVARVTS 182

Query: 197 NSLALINSYAS 205
           N+LAL+N++A+
Sbjct: 183 NALALVNNFAA 189

BLAST of CSPI05G06480 vs. Swiss-Prot
Match: PME2_ARATH (Pectinesterase 2 OS=Arabidopsis thaliana GN=PME2 PE=2 SV=2)

HSP 1 Score: 82.8 bits (203), Expect = 5.0e-15
Identity = 61/208 (29.33%), Postives = 118/208 (56.73%), Query Frame = 1

Query: 11  LLLLLIISDQTQILSVAASTLPRKSSAGIRTNTE----YVRTSCSTTSYPRLCYNSLSVY 70
           + LLL+ S    I+ +AA+T  +  +  I T +      +++ CS+T YP LC+++++  
Sbjct: 27  IALLLLAS----IVGIAATTTNQNKNQKITTLSSTSHAILKSVCSSTLYPELCFSAVAAT 86

Query: 71  AGKIKTNPKTLALAALHVNLAAARSSAASMRRL-AKTRGLRRRDASAISDCVEEVGDSVF 130
            GK  T+ K +  A+L++   A + +  ++++L AK +GL  R+ +A+ DC+E + +++ 
Sbjct: 87  GGKELTSQKEVIEASLNLTTKAVKHNYFAVKKLIAKRKGLTPREVTALHDCLETIDETLD 146

Query: 131 ELQRAIRELGR-PRGYDFMGLISDIETWVSSALTDEETCMEGF----GGRRVNGVSVKAK 190
           EL  A+ +L + P+         D++T +SSA+T++ TC++GF      R+V    +K +
Sbjct: 147 ELHVAVEDLHQYPKQKSLRKHADDLKTLISSAITNQGTCLDGFSYDDADRKVRKALLKGQ 206

Query: 191 VRRHIVRVAHLTSNSLALINSYASSAAA 209
                V V H+ SN+LA+I +   +  A
Sbjct: 207 -----VHVEHMCSNALAMIKNMTETDIA 225

BLAST of CSPI05G06480 vs. Swiss-Prot
Match: PMEU1_SOLLC (Pectinesterase/pectinesterase inhibitor U1 OS=Solanum lycopersicum GN=PMEU1 PE=2 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 4.7e-13
Identity = 53/174 (30.46%), Postives = 101/174 (58.05%), Query Frame = 1

Query: 46  VRTSCSTTSYPRLCYNSL---SVYAGKIKTNPKTLALAALHVNLAAARSSAASMRRLAKT 105
           V+++CS T +P LCY+++   S ++ K+ T+ K +   +L++ + A R +  +++ L KT
Sbjct: 66  VKSACSNTLHPELCYSAIVNVSDFSKKV-TSQKDVIELSLNITVKAVRRNYYAVKELIKT 125

Query: 106 R-GLRRRDASAISDCVEEVGDSVFELQRAIRELGR-PRGYDFMGLISDIETWVSSALTDE 165
           R GL  R+  A+ DC+E + +++ EL  A+ +L   P        + D++T +SSA+T++
Sbjct: 126 RKGLTPREKVALHDCLETMDETLDELHTAVEDLELYPNKKSLKEHVEDLKTLISSAITNQ 185

Query: 166 ETCMEGFGGRRVNGVSVKAKVRRHIVR----VAHLTSNSLALINSYASSAAAEE 211
           ETC++GF     +      KVR+ +++    V  + SN+LA+I +   +  A E
Sbjct: 186 ETCLDGFSHDEAD-----KKVRKVLLKGQKHVEKMCSNALAMICNMTDTDIANE 233

BLAST of CSPI05G06480 vs. Swiss-Prot
Match: PME3_ARATH (Pectinesterase/pectinesterase inhibitor 3 OS=Arabidopsis thaliana GN=PME3 PE=2 SV=2)

HSP 1 Score: 74.7 bits (182), Expect = 1.4e-12
Identity = 58/209 (27.75%), Postives = 109/209 (52.15%), Query Frame = 1

Query: 2   ENPLPTLLPLLLLLIISDQTQILSVAASTLPRKSSAGIRTNTEYVRTSCSTTSYPRLCYN 61
           +N    LL   + L+       +S  AS    K +    ++   +R+SCS+T YP LC +
Sbjct: 16  KNKKLVLLSAAVALLFVAAVAGISAGASKANEKRTLSPSSHA-VLRSSCSSTRYPELCIS 75

Query: 62  SLSVYAGKIKTNPKTLALAALHVNLAAARSSAASMRRLAKTR-GLRRRDASAISDCVEEV 121
           ++    G   T+ K +  A++++ + A   +  ++++L K R GL  R+ +A+ DC+E +
Sbjct: 76  AVVTAGGVELTSQKDVIEASVNLTITAVEHNYFTVKKLIKKRKGLTPREKTALHDCLETI 135

Query: 122 GDSVFELQRAIRELG-RPRGYDFMGLISDIETWVSSALTDEETCMEGFGGRRVNGVSVKA 181
            +++ EL   + +L   P          D++T +SSA+T++ETC++GF     +    KA
Sbjct: 136 DETLDELHETVEDLHLYPTKKTLREHAGDLKTLISSAITNQETCLDGFSHDDADKQVRKA 195

Query: 182 KVRRHIVRVAHLTSNSLALINSYASSAAA 209
            ++  I  V H+ SN+LA+I +   +  A
Sbjct: 196 LLKGQI-HVEHMCSNALAMIKNMTDTDIA 222

BLAST of CSPI05G06480 vs. Swiss-Prot
Match: PME3_CITSI (Pectinesterase 3 OS=Citrus sinensis PE=1 SV=1)

HSP 1 Score: 74.3 bits (181), Expect = 1.8e-12
Identity = 50/184 (27.17%), Postives = 99/184 (53.80%), Query Frame = 1

Query: 23  ILSVAASTLPRKSSA--GIRTNTEYVRTSCSTTSYPRLCYNSLSVY--AGKIKTNPKTLA 82
           ++ + A    RK+S   G   +   +++SCS+T YP LC+++++    A K  T+ K + 
Sbjct: 44  VIGIVAGVNSRKNSGDNGNEPHHAILKSSCSSTRYPDLCFSAIAAVPEASKKVTSQKDVI 103

Query: 83  LAALHVNLAAARSSAASMRRLAKTRGLRRRDASAISDCVEEVGDSVFELQRAIRELGR-P 142
             +L++   A   +   +++L K   L +R+  A+ DC+E + +++ EL +A+ +L   P
Sbjct: 104 EMSLNITTTAVEHNYFGIQKLLKRTNLTKREKVALHDCLETIDETLDELHKAVEDLEEYP 163

Query: 143 RGYDFMGLISDIETWVSSALTDEETCMEGFGGRRVNGVSVKAKVRRHIVRVAHLTSNSLA 202
                     D++T +S+A+T++ TC++GF     N   V+  +    V V  + SN+LA
Sbjct: 164 NKKSLSQHADDLKTLMSAAMTNQGTCLDGFSHDDAN-KHVRDALSDGQVHVEKMCSNALA 223

BLAST of CSPI05G06480 vs. TrEMBL
Match: A0A0A0KP63_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G160150 PE=4 SV=1)

HSP 1 Score: 275.0 bits (702), Expect = 7.7e-71
Identity = 144/146 (98.63%), Postives = 145/146 (99.32%), Query Frame = 1

Query: 69  KIKTNPKTLALAALHVNLAAARSSAASMRRLAKTRGLRRRDASAISDCVEEVGDSVFELQ 128
           +IKTNPKTLALAALHVNLAAARSSAASMRRLAKTRGLRRRDASAISDCVEEVGDSVFELQ
Sbjct: 176 QIKTNPKTLALAALHVNLAAARSSAASMRRLAKTRGLRRRDASAISDCVEEVGDSVFELQ 235

Query: 129 RAIRELGRPRGYDFMGLISDIETWVSSALTDEETCMEGFGGRRVNGVSVKAKVRRHIVRV 188
           RAIRELGRPRGYDFMGLISDIETWVSSALTDEETCMEGFGGRRVNGVSVKAKVRRHIVRV
Sbjct: 236 RAIRELGRPRGYDFMGLISDIETWVSSALTDEETCMEGFGGRRVNGVSVKAKVRRHIVRV 295

Query: 189 AHLTSNSLALINSYASSAAAEEGVLP 215
           AHLTSNSLALINSYASSAA EEGVLP
Sbjct: 296 AHLTSNSLALINSYASSAAVEEGVLP 321

BLAST of CSPI05G06480 vs. TrEMBL
Match: A0A067JNZ4_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_20773 PE=4 SV=1)

HSP 1 Score: 191.8 bits (486), Expect = 8.5e-46
Identity = 103/196 (52.55%), Postives = 144/196 (73.47%), Query Frame = 1

Query: 11  LLLLLIISDQTQILSVAAST-LPRKSSAGIRTNTEYVRTSCSTTSYPRLCYNSLSVYAGK 70
           L+ +L +   T I S+  S  LP        TNT+Y++TSC+ T+YPRLCY+SLS+YA K
Sbjct: 11  LVAILTLQFSTHINSIEPSKPLPN------NTNTQYIKTSCTNTTYPRLCYHSLSIYASK 70

Query: 71  IKTNPKTLALAALHVNLAAARSSAASMRRLAKTRGLRRRDASAISDCVEEVGDSVFELQR 130
           IKTNPK L   AL+V L A +S++  M+RL++  GL+RR+A+A+ DCVE VGDSV+ELQR
Sbjct: 71  IKTNPKLLTNIALNVTLKATKSTSRLMKRLSRIHGLKRREAAALVDCVEVVGDSVYELQR 130

Query: 131 AIRELGRPRGYDFMGLISDIETWVSSALTDEETCMEGFGGRRVNGVSVKAKVRRHIVRVA 190
           +I E+G+  G  F  +I DI+TWVS+ALTD++TCM+GF    +N   VK   R+H++++A
Sbjct: 131 SIGEMGQAEGSKFAQVIDDIQTWVSAALTDDDTCMDGFSEVSMNR-KVKIIARKHLLKIA 190

Query: 191 HLTSNSLALINSYASS 206
           HLTSN+LALIN++ASS
Sbjct: 191 HLTSNALALINNFASS 199

BLAST of CSPI05G06480 vs. TrEMBL
Match: F6HHS0_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_00s0323g00060 PE=4 SV=1)

HSP 1 Score: 191.0 bits (484), Expect = 1.5e-45
Identity = 98/171 (57.31%), Postives = 132/171 (77.19%), Query Frame = 1

Query: 35  SSAGIRTNTEYVRTSCSTTSYPRLCYNSLSVYAGKIKTNPKTLALAALHVNLAAARSSAA 94
           SS+   TNT+Y++TSC  T+YP LCY+SLS+YA KI+T+PK LA  AL V  ++ARS+  
Sbjct: 26  SSSAAHTNTDYIKTSCLATTYPHLCYDSLSIYANKIQTSPKRLATTALSVASSSARSTLV 85

Query: 95  SMRRLAKTRGLRRRDASAISDCVEEVGDSVFELQRAIRELGRPRGYDFMGLISDIETWVS 154
           SM++L+KT GL+ R+ASA+ DCVEEV DSV EL ++I E+G   G DF   + +I+TWVS
Sbjct: 86  SMKQLSKTHGLKPREASAMIDCVEEVADSVDELHKSIGEMGHAGGPDFEFRMGNIQTWVS 145

Query: 155 SALTDEETCMEGFGGRRVNGVSVKAKVRRHIVRVAHLTSNSLALINSYASS 206
           +ALTDEETC +GF GR +NG ++K  V+RHI +VA LTSN+LAL+N YAS+
Sbjct: 146 AALTDEETCTDGFAGRAMNG-NLKKTVQRHINKVARLTSNALALVNKYAST 195

BLAST of CSPI05G06480 vs. TrEMBL
Match: M5WVK9_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa011699mg PE=4 SV=1)

HSP 1 Score: 186.4 bits (472), Expect = 3.6e-44
Identity = 108/202 (53.47%), Postives = 146/202 (72.28%), Query Frame = 1

Query: 8   LLPLLLLLIISDQTQILSVAASTLPRKSSAGIRTNTEYVRTSCSTTSYPRLCYNSLSVYA 67
           L+P LLL +      IL +     P  ++   +TNT+Y++ SCS T YPRLCY+SLS+YA
Sbjct: 5   LMPALLLAL-----HILQLFNHINPCTATG--KTNTQYIKRSCSVTIYPRLCYHSLSIYA 64

Query: 68  GKIKTNPKTLALAALHVNLAAARSSAASMRRLAKTRGLRRRDASAISDCVEEVGDSVFEL 127
           GKIKT+PK LA  AL+V LAA + S+  MRRL++  GL+  + +A+ DC+EE+GD++ EL
Sbjct: 65  GKIKTDPKVLAHTALNVTLAATQESSVIMRRLSEIHGLKPIETAAVLDCMEEIGDAILEL 124

Query: 128 QRAIRELGR-PRGYDFMGLISDIETWVSSALTDEETCMEGFGGRRVNGVSVKAKVRRHIV 187
           Q+++ ELG   RG +F   +SDI+TWVS+ALTDE+TCM+GF    + G  V+A VRR+ V
Sbjct: 125 QQSLDELGHVVRGSNFWFQMSDIQTWVSAALTDEDTCMDGFEEHHLRG-RVEAVVRRYTV 184

Query: 188 RVAHLTSNSLALINSYASSAAA 209
            VAHLTSN+LALINSYASS AA
Sbjct: 185 NVAHLTSNALALINSYASSKAA 198

BLAST of CSPI05G06480 vs. TrEMBL
Match: A0A061G5H5_THECC (Plant invertase/pectin methylesterase inhibitor superfamily protein, putative OS=Theobroma cacao GN=TCM_016048 PE=4 SV=1)

HSP 1 Score: 185.7 bits (470), Expect = 6.1e-44
Identity = 100/195 (51.28%), Postives = 145/195 (74.36%), Query Frame = 1

Query: 11  LLLLLIISDQTQILSVAASTLPRKSSAGIRTNTEYVRTSCSTTSYPRLCYNSLSVYAGKI 70
           L +LLII   T   +++++  P  S    + N EY+++SCSTT+YPRLCY SLS+YA KI
Sbjct: 10  LAILLIILQFTTYTALSSADTPFHS----QRNIEYIKSSCSTTTYPRLCYRSLSIYASKI 69

Query: 71  KTNPKTLALAALHVNLAAARSSAASMRRLAKTRGLRRRDASAISDCVEEVGDSVFELQRA 130
            T+P+ L   AL+V L A++S++  M ++++  GLR R A+A++DC+E +GDSV ELQ++
Sbjct: 70  NTSPRLLVHTALNVTLKASKSTSRLMIKISRIHGLRPRVAAAMADCIEVMGDSVDELQQS 129

Query: 131 IRELGRPRGYDFMGLISDIETWVSSALTDEETCMEGFGGRRVNGVSVKAKVRRHIVRVAH 190
           I ELG  R  +F   +SDI+TWVS+ALTDE+TCM+GF G+ +NG + K  VR+ IV++AH
Sbjct: 130 IGELGHIRSSNFSLTMSDIQTWVSAALTDEDTCMDGFAGKAMNGYA-KTMVRKRIVKIAH 189

Query: 191 LTSNSLALINSYASS 206
           LTSN+LALIN+YAS+
Sbjct: 190 LTSNALALINNYAST 199

BLAST of CSPI05G06480 vs. TAIR10
Match: AT5G62360.1 (AT5G62360.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 157.5 bits (397), Expect = 9.0e-39
Identity = 84/164 (51.22%), Postives = 126/164 (76.83%), Query Frame = 1

Query: 41  TNTEYVRTSCSTTSYPRLCYNSLSVYAGKIKTNPKTLALAALHVNLAAARSSAASMRRLA 100
           TNTE+V++SC+ T+YPRLC++SLS +A  I+T+PK +A AAL++ LA+A+ ++A M RL+
Sbjct: 39  TNTEFVKSSCTFTTYPRLCFSSLSTHASLIQTSPKLMAHAALNITLASAKVTSAMMVRLS 98

Query: 101 KTRGLRRRDASAISDCVEEVGDSVFELQRAIRELGRPRGYDFMGLISDIETWVSSALTDE 160
            +R L+ ++ SA+ DCVEE+GD++ EL+++I E+ +  G ++   ISDI+TWVS+ALTD 
Sbjct: 99  NSR-LKPKEVSAMRDCVEELGDTLEELRKSIGEMCQLSGSNYEVYISDIQTWVSAALTDV 158

Query: 161 ETCMEGFGGRRVNGVSVKAKVRRHIVRVAHLTSNSLALINSYAS 205
            TC +GF G  ++G  VK  VR  I+ +AHLTSN+LALIN +AS
Sbjct: 159 NTCTDGFEGEDMDG-KVKVLVRGRILVIAHLTSNALALINHFAS 200

BLAST of CSPI05G06480 vs. TAIR10
Match: AT1G62760.1 (AT1G62760.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 155.6 bits (392), Expect = 3.4e-38
Identity = 88/186 (47.31%), Postives = 129/186 (69.35%), Query Frame = 1

Query: 25  SVAASTLPRKSSAGI--RTNTEYVRTSCSTTSYPRLCYNSLSVYAGKIKTNPKTLALAAL 84
           S  +S  P  SS+    +TN +Y++TSC+ T Y  +CYNSLS YA  I++NP+ LA+ AL
Sbjct: 125 SPLSSLSPSSSSSTYSNQTNLDYIKTSCNITLYKTICYNSLSPYASTIRSNPQKLAVIAL 184

Query: 85  HVNLAAARSSAASMRRLAKTRGLRRRDASAISDCVEEVGDSVFELQRAIRELGRPRGYD- 144
           ++ L++A+S++  ++ ++   GL R +  A++DCVEE+GDSV  LQ +IREL      D 
Sbjct: 185 NLTLSSAKSASKFVKNISHGGGLTRLEVVAVADCVEEIGDSVTSLQDSIRELDSINYKDS 244

Query: 145 --FMGLISDIETWVSSALTDEETCMEGFGGRRVNGVSVKAKVRRHIVRVAHLTSNSLALI 204
             F  ++SD+ETWVS+ALT+++TCM+GF    +   +VK  VRRH+V VA LTSN+LALI
Sbjct: 245 AKFEMVMSDVETWVSAALTNDDTCMDGFS---LVKTAVKDLVRRHVVEVARLTSNALALI 304

Query: 205 NSYASS 206
           N YAS+
Sbjct: 305 NMYAST 307

BLAST of CSPI05G06480 vs. TAIR10
Match: AT4G25260.1 (AT4G25260.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 146.7 bits (369), Expect = 1.6e-35
Identity = 80/185 (43.24%), Postives = 117/185 (63.24%), Query Frame = 1

Query: 24  LSVAASTLPRKSSAGIRTNTEYVRTSCSTTSYPRLCYNSLSVYAGKIKTNPKTLALAALH 83
           LS AA  + R        +TE+++ SC TTSYP  C+ SLS YA +IK  P+ LA  AL 
Sbjct: 16  LSTAAIVMARNLEEESSGDTEFIKASCETTSYPDRCFQSLSSYASEIKKQPRKLAETALA 75

Query: 84  VNLAAARSSAASMRRLAKTRGLRRRDASAISDCVEEVGDSVFELQRAIRELGR----PRG 143
           V++A A+S+   +  +   +G+ +R   A++DC+EE+GD+V  L  +++EL        G
Sbjct: 76  VSIARAKSAKTYVSEMTDYKGITKRQHEAVADCLEEMGDTVDRLSNSLKELKHLEEGDSG 135

Query: 144 YDFMGLISDIETWVSSALTDEETCMEGFGGRRVNGVSVKAKVRRHIVRVAHLTSNSLALI 203
            DF   +S++ TW S+ALTDE  CM+GFGG+ + G  +K+ +R HIV VA  TSN+LALI
Sbjct: 136 EDFWFCLSNVRTWTSAALTDETACMDGFGGKAMAG-ELKSLIRTHIVSVAEETSNALALI 195

Query: 204 NSYAS 205
           N +AS
Sbjct: 196 NDFAS 199

BLAST of CSPI05G06480 vs. TAIR10
Match: AT5G62350.1 (AT5G62350.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 137.9 bits (346), Expect = 7.4e-33
Identity = 81/198 (40.91%), Postives = 127/198 (64.14%), Query Frame = 1

Query: 12  LLLLIISDQTQILSVAASTLPRKSSAGIRTNTEYVRTSCSTTSYPRLCYNSLSVYAGKIK 71
           L +L+       L + A+T   ++ A  +    ++++SC TT+YP LC +SLSVYA  I+
Sbjct: 6   LFVLLSISYLLSLELTAATAASQTGASKKA-INFIQSSCKTTTYPALCVHSLSVYANDIQ 65

Query: 72  TNPKTLALAALHVNLAAARSSAASMRRLAKTRGLRRRDASAISDCVEEVGDSVFELQRAI 131
           T+PK LA  A+ V L+ A+S+   + RL + +GL++R+  AI DCVEE+ D+V  L +++
Sbjct: 66  TSPKRLAETAIAVTLSRAQSTKLFVSRLTRMKGLKKREVEAIKDCVEEMNDTVDRLTKSV 125

Query: 132 REL---GRPRGYD-FMGLISDIETWVSSALTDEETCMEGFGGRRVNGVSVKAKVRRHIVR 191
           +EL   G  +  D F   +S+ +TW S+ALTDE TC +GF GR ++G  +K  VR  I+ 
Sbjct: 126 QELKLCGSAKDQDQFAYHMSNAQTWTSAALTDENTCSDGFSGRVMDG-RIKNSVRARIMN 185

Query: 192 VAHLTSNSLALINSYASS 206
           V H TSN+L+LIN++A +
Sbjct: 186 VGHETSNALSLINAFAKT 201

BLAST of CSPI05G06480 vs. TAIR10
Match: AT4G12390.1 (AT4G12390.1 pectin methylesterase inhibitor 1)

HSP 1 Score: 129.4 bits (324), Expect = 2.6e-30
Identity = 80/200 (40.00%), Postives = 122/200 (61.00%), Query Frame = 1

Query: 11  LLLLLIISDQTQILSVAASTLPRKSSAGIRTNTEYVRTSCSTTSYPRLCYNSLSVYAGKI 70
           LLL L +  Q+ I   ++S  P  S         ++ +SC  T Y  LC   L+ +A KI
Sbjct: 12  LLLFLPLLCQSTIAKPSSSPNPSSS-------INFIVSSCRVTRYQTLCVKCLAAFADKI 71

Query: 71  KTNPKTLALAALHVNLAAARSSAASMRRLAKTRGLRRRDASAISDCVEEVGDSVFELQRA 130
           + N   LA  AL V L   +S+   + +L K R ++RR+  A+ DCVE +GD +  L ++
Sbjct: 72  RRNENQLAQTALAVTLVRVQSTTIYVGKLTKARRIKRREYLAVKDCVENLGDGLEMLAQS 131

Query: 131 IREL------GRPRGYDFMGLISDIETWVSSALTDEETCMEGFGGRRVNGVSVKAKVRRH 190
           +REL      GR R  +F+  +S++ETWVS+ALTDE TC++GF G+ ++GV VK+ +RR 
Sbjct: 132 MRELKQVGRSGRDRD-EFLWRLSNVETWVSAALTDETTCLDGFDGKVMDGV-VKSAIRRR 191

Query: 191 IVRVAHLTSNSLALINSYAS 205
           +V VA +TSN+LAL+N +A+
Sbjct: 192 VVHVARVTSNALALVNRFAA 202

BLAST of CSPI05G06480 vs. NCBI nr
Match: gi|449459754|ref|XP_004147611.1| (PREDICTED: 21 kDa protein isoform X1 [Cucumis sativus])

HSP 1 Score: 395.2 bits (1014), Expect = 7.3e-107
Identity = 213/215 (99.07%), Postives = 213/215 (99.07%), Query Frame = 1

Query: 1   MENPLPTLLPLLLL-LIISDQTQILSVAASTLPRKSSAGIRTNTEYVRTSCSTTSYPRLC 60
           MENPLPTLLPLLLL LIISDQTQILSVAASTLPRKSSAGIRTNTEYVRTSCSTTSYPRLC
Sbjct: 1   MENPLPTLLPLLLLLLIISDQTQILSVAASTLPRKSSAGIRTNTEYVRTSCSTTSYPRLC 60

Query: 61  YNSLSVYAGKIKTNPKTLALAALHVNLAAARSSAASMRRLAKTRGLRRRDASAISDCVEE 120
           YNSLSVYAGKIKTNPKTLALAALHVNLAAARSSAASMRRLAKTRGLRRRDASAISDCVEE
Sbjct: 61  YNSLSVYAGKIKTNPKTLALAALHVNLAAARSSAASMRRLAKTRGLRRRDASAISDCVEE 120

Query: 121 VGDSVFELQRAIRELGRPRGYDFMGLISDIETWVSSALTDEETCMEGFGGRRVNGVSVKA 180
           VGDSVFELQRAIRELGRPRGYDFMGLISDIETWVSSALTDEETCMEGFGGRRVNGVSVKA
Sbjct: 121 VGDSVFELQRAIRELGRPRGYDFMGLISDIETWVSSALTDEETCMEGFGGRRVNGVSVKA 180

Query: 181 KVRRHIVRVAHLTSNSLALINSYASSAAAEEGVLP 215
           KVRRHIVRVAHLTSNSLALINSYASSAA EEGVLP
Sbjct: 181 KVRRHIVRVAHLTSNSLALINSYASSAAVEEGVLP 215

BLAST of CSPI05G06480 vs. NCBI nr
Match: gi|778699944|ref|XP_011654789.1| (PREDICTED: 21 kDa protein isoform X3 [Cucumis sativus])

HSP 1 Score: 392.5 bits (1007), Expect = 4.7e-106
Identity = 212/214 (99.07%), Postives = 212/214 (99.07%), Query Frame = 1

Query: 1   MENPLPTLLPLLLLLIISDQTQILSVAASTLPRKSSAGIRTNTEYVRTSCSTTSYPRLCY 60
           MENPLPTLL LLLLLIISDQTQILSVAASTLPRKSSAGIRTNTEYVRTSCSTTSYPRLCY
Sbjct: 1   MENPLPTLL-LLLLLIISDQTQILSVAASTLPRKSSAGIRTNTEYVRTSCSTTSYPRLCY 60

Query: 61  NSLSVYAGKIKTNPKTLALAALHVNLAAARSSAASMRRLAKTRGLRRRDASAISDCVEEV 120
           NSLSVYAGKIKTNPKTLALAALHVNLAAARSSAASMRRLAKTRGLRRRDASAISDCVEEV
Sbjct: 61  NSLSVYAGKIKTNPKTLALAALHVNLAAARSSAASMRRLAKTRGLRRRDASAISDCVEEV 120

Query: 121 GDSVFELQRAIRELGRPRGYDFMGLISDIETWVSSALTDEETCMEGFGGRRVNGVSVKAK 180
           GDSVFELQRAIRELGRPRGYDFMGLISDIETWVSSALTDEETCMEGFGGRRVNGVSVKAK
Sbjct: 121 GDSVFELQRAIRELGRPRGYDFMGLISDIETWVSSALTDEETCMEGFGGRRVNGVSVKAK 180

Query: 181 VRRHIVRVAHLTSNSLALINSYASSAAAEEGVLP 215
           VRRHIVRVAHLTSNSLALINSYASSAA EEGVLP
Sbjct: 181 VRRHIVRVAHLTSNSLALINSYASSAAVEEGVLP 213

BLAST of CSPI05G06480 vs. NCBI nr
Match: gi|659074645|ref|XP_008437716.1| (PREDICTED: 21 kDa protein-like, partial [Cucumis melo])

HSP 1 Score: 287.7 bits (735), Expect = 1.6e-74
Identity = 155/159 (97.48%), Postives = 155/159 (97.48%), Query Frame = 1

Query: 11  LLLLLIISDQTQILSVAASTLPRKSSAGIRTNTEYVRTSCSTTSYPRLCYNSLSVYAGKI 70
           LLLLLIISDQTQI SVAASTLP KS A IRTNTEYVRTSCSTTSYPRLCYNSLSVYAGKI
Sbjct: 65  LLLLLIISDQTQIFSVAASTLP-KSPARIRTNTEYVRTSCSTTSYPRLCYNSLSVYAGKI 124

Query: 71  KTNPKTLALAALHVNLAAARSSAASMRRLAKTRGLRRRDASAISDCVEEVGDSVFELQRA 130
           KTNPKTLALAALHVNLAAARSSAASMRRLAKTRGLRRRDASAISDCVEEVGDSVFELQRA
Sbjct: 125 KTNPKTLALAALHVNLAAARSSAASMRRLAKTRGLRRRDASAISDCVEEVGDSVFELQRA 184

Query: 131 IRELGRPRGYDFMGLISDIETWVSSALTDEETCMEGFGG 170
           IRELGRPRGYDFMGLISDIETWVSSALTDEETCMEGFGG
Sbjct: 185 IRELGRPRGYDFMGLISDIETWVSSALTDEETCMEGFGG 222

BLAST of CSPI05G06480 vs. NCBI nr
Match: gi|700195035|gb|KGN50212.1| (hypothetical protein Csa_5G160150 [Cucumis sativus])

HSP 1 Score: 275.0 bits (702), Expect = 1.1e-70
Identity = 144/146 (98.63%), Postives = 145/146 (99.32%), Query Frame = 1

Query: 69  KIKTNPKTLALAALHVNLAAARSSAASMRRLAKTRGLRRRDASAISDCVEEVGDSVFELQ 128
           +IKTNPKTLALAALHVNLAAARSSAASMRRLAKTRGLRRRDASAISDCVEEVGDSVFELQ
Sbjct: 176 QIKTNPKTLALAALHVNLAAARSSAASMRRLAKTRGLRRRDASAISDCVEEVGDSVFELQ 235

Query: 129 RAIRELGRPRGYDFMGLISDIETWVSSALTDEETCMEGFGGRRVNGVSVKAKVRRHIVRV 188
           RAIRELGRPRGYDFMGLISDIETWVSSALTDEETCMEGFGGRRVNGVSVKAKVRRHIVRV
Sbjct: 236 RAIRELGRPRGYDFMGLISDIETWVSSALTDEETCMEGFGGRRVNGVSVKAKVRRHIVRV 295

Query: 189 AHLTSNSLALINSYASSAAAEEGVLP 215
           AHLTSNSLALINSYASSAA EEGVLP
Sbjct: 296 AHLTSNSLALINSYASSAAVEEGVLP 321

BLAST of CSPI05G06480 vs. NCBI nr
Match: gi|694367306|ref|XP_009362111.1| (PREDICTED: 21 kDa protein-like [Pyrus x bretschneideri])

HSP 1 Score: 195.7 bits (496), Expect = 8.5e-47
Identity = 114/208 (54.81%), Postives = 155/208 (74.52%), Query Frame = 1

Query: 8   LLPLLLLLIISDQTQILSVAASTLPRKSSAGIRTNTEYVRTSCSTTSYPRLCYNSLSVYA 67
           L+PL++LL + +  Q+ S  A+ LP KS      NT+Y++ SCS T+YP+LCYNSLS+YA
Sbjct: 6   LIPLVVLLALLN-LQLCS--ANELPSKS------NTQYIKRSCSVTTYPKLCYNSLSIYA 65

Query: 68  GKIKTNPKTLALAALHVNLAAARSSAASMRRLAKTRGLRRRDASAISDCVEEVGDSVFEL 127
           GKIKTNPK LA  AL+V +AA + +  +M+RL+KT GL   +A+A  DCVEE+GD+V E+
Sbjct: 66  GKIKTNPKVLARTALNVTIAATQETYVTMKRLSKTHGLNPIEAAAALDCVEEIGDAVEEV 125

Query: 128 QRAIRELGR-PRGYDFMGLISDIETWVSSALTDEETCMEGFGGRRVNGVSVKAKVRRHIV 187
           Q++I E G   RG +F   +SDI+TWVS+ALTDE+TCM+GF    ++G  V++ VRR+IV
Sbjct: 126 QKSIDEFGHVARGSNFWVQMSDIQTWVSAALTDEDTCMDGFDEHHMSG-RVESLVRRYIV 185

Query: 188 RVAHLTSNSLALINSYASSAAAEEGVLP 215
            VAHLTSN+LALINSYAS  A+ E +LP
Sbjct: 186 NVAHLTSNALALINSYAS--ASTEALLP 201

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
21KD_DAUCA1.8e-3341.8821 kDa protein OS=Daucus carota PE=2 SV=1[more]
PME2_ARATH5.0e-1529.33Pectinesterase 2 OS=Arabidopsis thaliana GN=PME2 PE=2 SV=2[more]
PMEU1_SOLLC4.7e-1330.46Pectinesterase/pectinesterase inhibitor U1 OS=Solanum lycopersicum GN=PMEU1 PE=2... [more]
PME3_ARATH1.4e-1227.75Pectinesterase/pectinesterase inhibitor 3 OS=Arabidopsis thaliana GN=PME3 PE=2 S... [more]
PME3_CITSI1.8e-1227.17Pectinesterase 3 OS=Citrus sinensis PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KP63_CUCSA7.7e-7198.63Uncharacterized protein OS=Cucumis sativus GN=Csa_5G160150 PE=4 SV=1[more]
A0A067JNZ4_JATCU8.5e-4652.55Uncharacterized protein OS=Jatropha curcas GN=JCGZ_20773 PE=4 SV=1[more]
F6HHS0_VITVI1.5e-4557.31Putative uncharacterized protein OS=Vitis vinifera GN=VIT_00s0323g00060 PE=4 SV=... [more]
M5WVK9_PRUPE3.6e-4453.47Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa011699mg PE=4 SV=1[more]
A0A061G5H5_THECC6.1e-4451.28Plant invertase/pectin methylesterase inhibitor superfamily protein, putative OS... [more]
Match NameE-valueIdentityDescription
AT5G62360.19.0e-3951.22 Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
AT1G62760.13.4e-3847.31 Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
AT4G25260.11.6e-3543.24 Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
AT5G62350.17.4e-3340.91 Plant invertase/pectin methylesterase inhibitor superfamily protein[more]
AT4G12390.12.6e-3040.00 pectin methylesterase inhibitor 1[more]
Match NameE-valueIdentityDescription
gi|449459754|ref|XP_004147611.1|7.3e-10799.07PREDICTED: 21 kDa protein isoform X1 [Cucumis sativus][more]
gi|778699944|ref|XP_011654789.1|4.7e-10699.07PREDICTED: 21 kDa protein isoform X3 [Cucumis sativus][more]
gi|659074645|ref|XP_008437716.1|1.6e-7497.48PREDICTED: 21 kDa protein-like, partial [Cucumis melo][more]
gi|700195035|gb|KGN50212.1|1.1e-7098.63hypothetical protein Csa_5G160150 [Cucumis sativus][more]
gi|694367306|ref|XP_009362111.1|8.5e-4754.81PREDICTED: 21 kDa protein-like [Pyrus x bretschneideri][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR006501Pectinesterase_inhib_dom
Vocabulary: Molecular Function
TermDefinition
GO:0004857enzyme inhibitor activity
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0005982 starch metabolic process
biological_process GO:0005985 sucrose metabolic process
cellular_component GO:0005575 cellular_component
molecular_function GO:0004857 enzyme inhibitor activity
molecular_function GO:0030599 pectinesterase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI05G06480.1CSPI05G06480.1mRNA


Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainGENE3DG3DSA:1.20.140.40coord: 42..201
score: 1.5
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 45..198
score: 3.6
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 41..198
score: 1.0
IPR006501Pectinesterase inhibitor domainTIGRFAMsTIGR01614TIGR01614coord: 31..202
score: 3.7
IPR006501Pectinesterase inhibitor domainunknownSSF101148Plant invertase/pectin methylesterase inhibitorcoord: 44..201
score: 1.57
NoneNo IPR availablePANTHERPTHR31080FAMILY NOT NAMEDcoord: 1..211
score: 3.3
NoneNo IPR availablePANTHERPTHR31080:SF8F23N19.12coord: 1..211
score: 3.3

The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
CSPI05G06480CSPI03G03060Wild cucumber (PI 183967)cpicpiB115
The following block(s) are covering this gene:
GeneOrganismBlock
CSPI05G06480Silver-seed gourdcarcpiB0902
CSPI05G06480Silver-seed gourdcarcpiB0962
CSPI05G06480Cucumber (Chinese Long) v3cpicucB269
CSPI05G06480Cucumber (Chinese Long) v3cpicucB268
CSPI05G06480Cucumber (Chinese Long) v3cpicucB285
CSPI05G06480Watermelon (97103) v2cpiwmbB349
CSPI05G06480Watermelon (97103) v2cpiwmbB372
CSPI05G06480Watermelon (97103) v2cpiwmbB404
CSPI05G06480Wax gourdcpiwgoB440
CSPI05G06480Wild cucumber (PI 183967)cpicpiB116
CSPI05G06480Cucumber (Gy14) v1cgycpiB386
CSPI05G06480Cucumber (Gy14) v1cgycpiB457
CSPI05G06480Cucumber (Gy14) v1cgycpiB487
CSPI05G06480Cucurbita maxima (Rimu)cmacpiB614
CSPI05G06480Cucurbita maxima (Rimu)cmacpiB853
CSPI05G06480Cucurbita moschata (Rifu)cmocpiB339
CSPI05G06480Cucurbita moschata (Rifu)cmocpiB606
CSPI05G06480Cucurbita moschata (Rifu)cmocpiB836
CSPI05G06480Cucumber (Chinese Long) v2cpicuB226
CSPI05G06480Cucumber (Chinese Long) v2cpicuB240
CSPI05G06480Melon (DHL92) v3.5.1cpimeB348
CSPI05G06480Melon (DHL92) v3.5.1cpimeB403
CSPI05G06480Watermelon (Charleston Gray)cpiwcgB368
CSPI05G06480Watermelon (Charleston Gray)cpiwcgB379
CSPI05G06480Watermelon (Charleston Gray)cpiwcgB414
CSPI05G06480Watermelon (97103) v1cpiwmB381
CSPI05G06480Watermelon (97103) v1cpiwmB416
CSPI05G06480Watermelon (97103) v1cpiwmB412
CSPI05G06480Cucurbita pepo (Zucchini)cpecpiB742
CSPI05G06480Cucurbita pepo (Zucchini)cpecpiB874
CSPI05G06480Bottle gourd (USVL1VR-Ls)cpilsiB322
CSPI05G06480Bottle gourd (USVL1VR-Ls)cpilsiB379
CSPI05G06480Bottle gourd (USVL1VR-Ls)cpilsiB390
CSPI05G06480Melon (DHL92) v3.6.1cpimedB399
CSPI05G06480Cucumber (Gy14) v2cgybcpiB130
CSPI05G06480Cucumber (Gy14) v2cgybcpiB131
CSPI05G06480Cucumber (Gy14) v2cgybcpiB226