CSPI05G02460 (gene) Wild cucumber (PI 183967)

NameCSPI05G02460
Typegene
OrganismCucumis sativus (Wild cucumber (PI 183967))
DescriptionProtoporphyrinogen oxidase
LocationChr5 : 3104993 .. 3109779 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTCATATTGAGACAAAATTCCTTCAGAAATGTTGTTGTAGATAGATAACACATCCCTTCTTCAACTTCACTCTTCATCATCCTCTCTTCACCCTCCAAAACTCTCACTTCCCTCTCCCCCATGGCCACCGGCTCCACACTCCTCACCGACCTCCCTTTCCGGCGCCCTCACCCTCTCACTCTTCTCCGCCCCTCAAACATTCCTTCTTTTCACCCACTCCACATCTCTCTCCAAAACAATCGCCTTCGCTCCTATTTCCGATGTTCAATTGCCGAGGGCTCCACCGCTCTTTCCCCTTCCAATGCCTCTTCCCAGTCTTCTATCCTGGATTGTGTGGTCGTCGGTGCTGGAATTAGTGGCCTCTGCATCGCTCAGGCTCTTGCTACCAAACACCCCGATGTTGCCCCTAACATCATTGTCACCGAGGCCAAGGATCGCGTTGGAGGCAACATCACCACGGTTGAGAGAGATGGATATCTCTGGGAAGAAGGCCCTAATAGCTTCCAACCTTCCGATCCTATTCTCACCATGGTGGTTAGTATCATTCGTTCGACCAAAACTTTAGTTTCTAACTAAATTTGGGGTCAAATTCGAGAGGAAACTTTAAGAATGTATAACTTCTATAGTAAATTTTATAATCTGAGACGAAGTATGGAGGGTAAGGATCTTCTCTTACCTTCAAGCCGCCATCGTCTAAGGCAAATTATTAGCCTTAGCTGATTGACCGTTTCTATTTCCCTATTTTGATCGTTAATTATGAGACAAGCAGGCCTCTTCTGACCATACATGTTCTAGGTGATTCCGTGCTAAGAGTTTTATATATGCATGATGGAGGAAGGTGCCCACTCTAAGTTGCGTAGTTTTGTTGATTGTAGTTCCATGGGATTTATGCTCTAACTAGAATCAGTGTAGTCGGACATTCTTCGTATTCACTACCATTTACTACCGAGGGATTTAATCTTAGATTTTTATATTGTGTCAGGTGCATGAAAGGTTTATATGTATTGTCAGGAAGTGATTTAGTTTGTGTACTTTTCCCTTGGTTTATTTGTTAATTCTTATACAATGGCAGGTGGATAGTGGCTTAAAAGATGATTTAGTTCTGGGAGACCCAGATGCACCTCGATTTGTATTGTGGAATGGAAAGCTCAGACCAGTGCCTGCGAAACCTAATGATCTACCTTTCTTTGACCTGATGAGCATTGGTGGAAAAATCAGAGCAGGCTTTGGTGCCCTGGGCATTCGCCCACCTCCTCCAGTTTGTCTTCTCAATTCTTTTTCTCTATATTGAAGTACATTGTCACTTTTTACTTCCCTCATCAATCTCAGAAAAACAAATATTTATTGTTTTTAGGTTCTCATATGTTGTAACACTAACAAAGGTAACAGGGTCGAGAGGAATCAGTTGAAGAATTTGTCCGTCGGAACCTTGGCAATGAAGTTTTTGAACGTTTGATAGAGCCATTTTGTTCTGGTGATTAACCTTATTCAATTAGATCTATATATCTATTTTTTCTTGCAAATTTTCTCTATCCATGTAATTCCTATTTTCTCGGTATCCAATTCATAGTAAGATCTAGAGAAATATTTGGACTTATGCAATGAAGACTTAAGAAATGAAGCTTAGGCTTGATGAGTAGGTTTACTAATTTGATAAACTACATTACCCTAACTTTTGAGTTCCGATTTTATGCTACAGTCAACACACTAAATTTTATTAATAGTGAACTAAACCATGGCATGAACTCAATTCTTATTATTCATTTGTTAGTAGAATCAAACGTAAATTACCATTTTGTTACTAGTAATAAAAAACACAATAGTAATGGCTAAACCATTTGTACTCTTTAGGAAAATTATAATAATTTAGTTTCAAGGTTATACTTCTTTGGTTGATTGTTTTACTCATTGTCAGTTGTTGACTTGAAACTATCTGTTCAGGTGTATACGCTGGTGACCCTTCAAAGCTAAGCATGAAAGCAGCTTTTGGTAAGGTTTGGAGGCTAGAGCAAAATGGTGGTAGTATTATTGGTGGGACTTTCAAAGCACTTCAAGAAAGGAATAAAACTACCAAACCACCAAGAGATCCGTAATGATTTAACTTCATGAAGTTTCTCCGTTTTTTTTCTTTTTTCCCTCCTCACCACTTTATGATATATTGTGTGTTTAATAACCATCTTCTTCTATTCCAGGCGTCTACCAAAGCCTAAGGGCCAAACTGTTGGATCTTTTCGGAAAGGACTTACCATGTTGCCAAATGCTATTTCTACTTGGTAAGCTTCAATTTTAAGACTAGTTTTCTGATTGCCTTTTTGACTTGGTCTTTAGATTTAACATTTCTTCAACTTTTTCTGTTGATTGTAGTTTGGGGAGTAAAGTAAAAGTATCTTGGAAGCTATCTAGTATCAGTAAAGTGGATGACGGAGGTTATAGTTTGACATACGAAACACCAGAAGGACTAGTCTCCATACTAAGCAGAAGTGTCATCATGACGGTTCCTTCTTATATTGCTGGCACTCTGTTGCGTCCAATCTCGGTAATTTAATTTCCTGGGACTTGAATGTGATTTTTGTCCTTTCAACAGTATTTTTATGATATTCATTTACATATATAGTTCCAGCTTTTCCATTGCATTCTTTATTGTTAATGATTGCAGCACCTTTTGCAATTCTAATCAACAGTTGAGTGTATCACTTGGAATTGACATTGTGGAAACACACTTCAACATTAGTCAGGTTGCAAAATAGATGTTTAACACTTTTTAGAGAATTTGGGATATTTGAGCGGAAGTTATGGGAGATTGTCAGGTATTTTTATTTTATTTTATTTTATTGATAAGAAAGAGAAACCATTTCACAGATGAGATGAAGTTATAAAAGATGGTGGGAGAGCCCAATATCCGAAACAAACAAATGAAGATTGCAAATAATTCTTCTAGCTGGTATAAAGATCACATAAAATACAACTATTGAAAGAAGCATTTAATTTTTTTATTTTTTTGAAAAGGTATTGTAATACTACTTGGGTGGGGGAATTTGAACTCGGGACCTTTTGTCCTCATATATATATAGGTGCCAGTTGAGCTAAGCTCATGTTGGCGAAACATATAACTACTTCATCAAGAAAGCTACAATCAATACATTAGGAAGAGAACTTGAAACTCAATTTACTCACAAGTGCAGAATATATGTATCTTGCAAATTCTGTACAGTAGTGCAGCTGATTGGTGCAAGAATAGGAAGTCCTGAATTTGTTGGTTGGTTCTCTGTTTTTAAGTTTGTTTATCTTCGATGATTCCTTCCCTGCAGGGGAAAGCTGCAGATGCACTTTCAAAATTTTATTATCCACCAGTTGCATCAGTGACCATATCATATCCAAAAGGAGCAATTAGGAAAGAATGCTTGATTGATGGTGAACTAAAGGGGTTTGGTCAATTGCACCCTCGTAGCCAGGGGGTGACTACTTTGGGTAAATCCGCATTCTTTACATTTCATCTGGCAGTCTGCCCTTAACTTCTGTTGACTTATATTTTGCCACACGATTCTTACAGCCCTAGCTGTTGGTCAGTTGGGTTGTTAAATTGCATTCAAATTCATGCTTCACTTTGTTGCAGGAACTATATACAGCTCATCACTTTTTCCTAATCGAGCGCCAGATGGAAGGGTATTGCTCTTGAACTACATTGGAGGGGCTACTAATACTGGAATTCTTTCTCAGGTATGTTTTTTTAACCACGGTTTGAAATTGAGCTTTCTGAATCATACGTGAAGTTTTGCTAGGAAATTGAAAAAGGCTTAATGTTGTATCTATATTTATATGCTTAGTAGTATTGATAATAATGGTACGATTAGGAAAGTTTCAACTACCATTTCTAACTCACTTTTTTAGATGAGAGACTGGTATAAATAACAAAAGAGATCTCTGCCTCGGGACTTGCCACTAAGTCAAGCAATTTGTAACCAACTTGGGTACTACATCTATAATTTTTCAAGGAACAGAAAATATGCAAATGTTGAATCATATTGATTTTCAAAATCTAGTAAAAGGAGCTTTTGAATTTGGCTTACATAAAAAATTGGTGTCCTTTGAATACCATTTTAAGTTCCATCTTCCATCCCTACTGTTTCTTATTTATAGCATTAGTTTTATTGTTATTATCTTGTAATTTGTAAGTACTTTTGTCACAACAGACAGAGAGCGAGCTCATAGAAGTAGTTGATCGGGATTTAAGAAAAATCCTCATAAACCCAAACGCAGAGGATCCTCTACCATTGAGCGTGAGGGTGTGGCCACAAGCCATTCCACAGTTCTTGATTGGCCATCTCGATGTTCTAGACACCGCCAAGGCCGGACTGAGAGAGGCTGGAATGGAGGGGCTATTTTTAGGTGGAAACTATGTATGCGGTGTGGCCTTGGGGAGATGTGTTGAGGGTGCCTATGAAGCTGCAGCTGAGGTAGCTGGTTTTCTGTCTAAAAAAGTGTACAAATAAATAGCTACTTCTTTTTGCTTTCTACCATTAGTAGGCACATAACCAAACTGATGGGCTCTTCACGCCATTGAGTTGTAACTTGTAAGGGATGGCGATCTTGCTTTGCAGATATGGTCTTTTATCTTGATTTTCGATTTGTTTTATTGTAGCAATAGTTGTAATCCTATTTTTAGGGTGAATCCTAACATGTTGAGCTCTCTAAAACTTCTTATGGCTCGACTTTGAGTGAATTGGACTTTTTTAGGGATTGTTTGGGATATTAAATTGGTTATTCTAGTTTGTAGTTATTATATTCTA

mRNA sequence

ATGGCCACCGGCTCCACACTCCTCACCGACCTCCCTTTCCGGCGCCCTCACCCTCTCACTCTTCTCCGCCCCTCAAACATTCCTTCTTTTCACCCACTCCACATCTCTCTCCAAAACAATCGCCTTCGCTCCTATTTCCGATGTTCAATTGCCGAGGGCTCCACCGCTCTTTCCCCTTCCAATGCCTCTTCCCAGTCTTCTATCCTGGATTGTGTGGTCGTCGGTGCTGGAATTAGTGGCCTCTGCATCGCTCAGGCTCTTGCTACCAAACACCCCGATGTTGCCCCTAACATCATTGTCACCGAGGCCAAGGATCGCGTTGGAGGCAACATCACCACGGTTGAGAGAGATGGATATCTCTGGGAAGAAGGCCCTAATAGCTTCCAACCTTCCGATCCTATTCTCACCATGGTGGTGGATAGTGGCTTAAAAGATGATTTAGTTCTGGGAGACCCAGATGCACCTCGATTTGTATTGTGGAATGGAAAGCTCAGACCAGTGCCTGCGAAACCTAATGATCTACCTTTCTTTGACCTGATGAGCATTGGTGGAAAAATCAGAGCAGGCTTTGGTGCCCTGGGCATTCGCCCACCTCCTCCAGGTCGAGAGGAATCAGTTGAAGAATTTGTCCGTCGGAACCTTGGCAATGAAGTTTTTGAACGTTTGATAGAGCCATTTTGTTCTGGTGTATACGCTGGTGACCCTTCAAAGCTAAGCATGAAAGCAGCTTTTGGTAAGGTTTGGAGGCTAGAGCAAAATGGTGGTAGTATTATTGGTGGGACTTTCAAAGCACTTCAAGAAAGGAATAAAACTACCAAACCACCAAGAGATCCGCGTCTACCAAAGCCTAAGGGCCAAACTGTTGGATCTTTTCGGAAAGGACTTACCATGTTGCCAAATGCTATTTCTACTTGTTTGGGGAGTAAAGTAAAAGTATCTTGGAAGCTATCTAGTATCAGTAAAGTGGATGACGGAGGTTATAGTTTGACATACGAAACACCAGAAGGACTAGTCTCCATACTAAGCAGAAGTGTCATCATGACGGTTCCTTCTTATATTGCTGGCACTCTGTTGCGTCCAATCTCGGGGAAAGCTGCAGATGCACTTTCAAAATTTTATTATCCACCAGTTGCATCAGTGACCATATCATATCCAAAAGGAGCAATTAGGAAAGAATGCTTGATTGATGGTGAACTAAAGGGGTTTGGTCAATTGCACCCTCGTAGCCAGGGGGTGACTACTTTGGGAACTATATACAGCTCATCACTTTTTCCTAATCGAGCGCCAGATGGAAGGGTATTGCTCTTGAACTACATTGGAGGGGCTACTAATACTGGAATTCTTTCTCAGACAGAGAGCGAGCTCATAGAAGTAGTTGATCGGGATTTAAGAAAAATCCTCATAAACCCAAACGCAGAGGATCCTCTACCATTGAGCGTGAGGGTGTGGCCACAAGCCATTCCACAGTTCTTGATTGGCCATCTCGATGTTCTAGACACCGCCAAGGCCGGACTGAGAGAGGCTGGAATGGAGGGGCTATTTTTAGGTGGAAACTATGTATGCGGTGTGGCCTTGGGGAGATGTGTTGAGGGTGCCTATGAAGCTGCAGCTGAGGTAGCTGGTTTTCTGTCTAAAAAAGTGTACAAATAA

Coding sequence (CDS)

ATGGCCACCGGCTCCACACTCCTCACCGACCTCCCTTTCCGGCGCCCTCACCCTCTCACTCTTCTCCGCCCCTCAAACATTCCTTCTTTTCACCCACTCCACATCTCTCTCCAAAACAATCGCCTTCGCTCCTATTTCCGATGTTCAATTGCCGAGGGCTCCACCGCTCTTTCCCCTTCCAATGCCTCTTCCCAGTCTTCTATCCTGGATTGTGTGGTCGTCGGTGCTGGAATTAGTGGCCTCTGCATCGCTCAGGCTCTTGCTACCAAACACCCCGATGTTGCCCCTAACATCATTGTCACCGAGGCCAAGGATCGCGTTGGAGGCAACATCACCACGGTTGAGAGAGATGGATATCTCTGGGAAGAAGGCCCTAATAGCTTCCAACCTTCCGATCCTATTCTCACCATGGTGGTGGATAGTGGCTTAAAAGATGATTTAGTTCTGGGAGACCCAGATGCACCTCGATTTGTATTGTGGAATGGAAAGCTCAGACCAGTGCCTGCGAAACCTAATGATCTACCTTTCTTTGACCTGATGAGCATTGGTGGAAAAATCAGAGCAGGCTTTGGTGCCCTGGGCATTCGCCCACCTCCTCCAGGTCGAGAGGAATCAGTTGAAGAATTTGTCCGTCGGAACCTTGGCAATGAAGTTTTTGAACGTTTGATAGAGCCATTTTGTTCTGGTGTATACGCTGGTGACCCTTCAAAGCTAAGCATGAAAGCAGCTTTTGGTAAGGTTTGGAGGCTAGAGCAAAATGGTGGTAGTATTATTGGTGGGACTTTCAAAGCACTTCAAGAAAGGAATAAAACTACCAAACCACCAAGAGATCCGCGTCTACCAAAGCCTAAGGGCCAAACTGTTGGATCTTTTCGGAAAGGACTTACCATGTTGCCAAATGCTATTTCTACTTGTTTGGGGAGTAAAGTAAAAGTATCTTGGAAGCTATCTAGTATCAGTAAAGTGGATGACGGAGGTTATAGTTTGACATACGAAACACCAGAAGGACTAGTCTCCATACTAAGCAGAAGTGTCATCATGACGGTTCCTTCTTATATTGCTGGCACTCTGTTGCGTCCAATCTCGGGGAAAGCTGCAGATGCACTTTCAAAATTTTATTATCCACCAGTTGCATCAGTGACCATATCATATCCAAAAGGAGCAATTAGGAAAGAATGCTTGATTGATGGTGAACTAAAGGGGTTTGGTCAATTGCACCCTCGTAGCCAGGGGGTGACTACTTTGGGAACTATATACAGCTCATCACTTTTTCCTAATCGAGCGCCAGATGGAAGGGTATTGCTCTTGAACTACATTGGAGGGGCTACTAATACTGGAATTCTTTCTCAGACAGAGAGCGAGCTCATAGAAGTAGTTGATCGGGATTTAAGAAAAATCCTCATAAACCCAAACGCAGAGGATCCTCTACCATTGAGCGTGAGGGTGTGGCCACAAGCCATTCCACAGTTCTTGATTGGCCATCTCGATGTTCTAGACACCGCCAAGGCCGGACTGAGAGAGGCTGGAATGGAGGGGCTATTTTTAGGTGGAAACTATGTATGCGGTGTGGCCTTGGGGAGATGTGTTGAGGGTGCCTATGAAGCTGCAGCTGAGGTAGCTGGTTTTCTGTCTAAAAAAGTGTACAAATAA
BLAST of CSPI05G02460 vs. Swiss-Prot
Match: PPOC_ARATH (Protoporphyrinogen oxidase 1, chloroplastic OS=Arabidopsis thaliana GN=PPOX1 PE=1 SV=1)

HSP 1 Score: 823.9 bits (2127), Expect = 1.0e-237
Identity = 412/540 (76.30%), Postives = 457/540 (84.63%), Query Frame = 1

Query: 19  LTLLRPSN---IPSFHPLHISLQNNRLRSY----FRCSIAEGSTALSPSNASSQSSIL-- 78
           L+LLRP+    +PSF     S  N RL  Y     RCS+A G T  S        + +  
Sbjct: 3   LSLLRPTTQSLLPSF-----SKPNLRLNVYKPLRLRCSVAGGPTVGSSKIEGGGGTTITT 62

Query: 79  DCVVVGAGISGLCIAQALATKHPDVAPNIIVTEAKDRVGGNITTVERDGYLWEEGPNSFQ 138
           DCV+VG GISGLCIAQALATKHPD APN+IVTEAKDRVGGNI T E +G+LWEEGPNSFQ
Sbjct: 63  DCVIVGGGISGLCIAQALATKHPDAAPNLIVTEAKDRVGGNIITREENGFLWEEGPNSFQ 122

Query: 139 PSDPILTMVVDSGLKDDLVLGDPDAPRFVLWNGKLRPVPAKPNDLPFFDLMSIGGKIRAG 198
           PSDP+LTMVVDSGLKDDLVLGDP APRFVLWNGKLRPVP+K  DLPFFDLMSIGGKIRAG
Sbjct: 123 PSDPMLTMVVDSGLKDDLVLGDPTAPRFVLWNGKLRPVPSKLTDLPFFDLMSIGGKIRAG 182

Query: 199 FGALGIRPPPPGREESVEEFVRRNLGNEVFERLIEPFCSGVYAGDPSKLSMKAAFGKVWR 258
           FGALGIRP PPGREESVEEFVRRNLG+EVFERLIEPFCSGVYAGDPSKLSMKAAFGKVW+
Sbjct: 183 FGALGIRPSPPGREESVEEFVRRNLGDEVFERLIEPFCSGVYAGDPSKLSMKAAFGKVWK 242

Query: 259 LEQNGGSIIGGTFKALQERNKTTKPPRDPRLPKPKGQTVGSFRKGLTMLPNAISTCLGSK 318
           LEQNGGSIIGGTFKA+QER    K  RDPRLPKP+GQTVGSFRKGL MLP AIS  LGSK
Sbjct: 243 LEQNGGSIIGGTFKAIQERKNAPKAERDPRLPKPQGQTVGSFRKGLRMLPEAISARLGSK 302

Query: 319 VKVSWKLSSISKVDDGGYSLTYETPEGLVSILSRSVIMTVPSYIAGTLLRPISGKAADAL 378
           VK+SWKLS I+K++ GGY+LTYETP+GLVS+ S+SV+MTVPS++A  LLRP+S  AA+AL
Sbjct: 303 VKLSWKLSGITKLESGGYNLTYETPDGLVSVQSKSVVMTVPSHVASGLLRPLSESAANAL 362

Query: 379 SKFYYPPVASVTISYPKGAIRKECLIDGELKGFGQLHPRSQGVTTLGTIYSSSLFPNRAP 438
           SK YYPPVA+V+ISYPK AIR ECLIDGELKGFGQLHPR+QGV TLGTIYSSSLFPNRAP
Sbjct: 363 SKLYYPPVAAVSISYPKEAIRTECLIDGELKGFGQLHPRTQGVETLGTIYSSSLFPNRAP 422

Query: 439 DGRVLLLNYIGGATNTGILSQTESELIEVVDRDLRKILINPNAEDPLPLSVRVWPQAIPQ 498
            GR+LLLNYIGG+TNTGILS++E EL+E VDRDLRK+LI PN+ DPL L VRVWPQAIPQ
Sbjct: 423 PGRILLLNYIGGSTNTGILSKSEGELVEAVDRDLRKMLIKPNSTDPLKLGVRVWPQAIPQ 482

Query: 499 FLIGHLDVLDTAKAGLREAGMEGLFLGGNYVCGVALGRCVEGAYEAAAEVAGFLSKKVYK 550
           FL+GH D+LDTAK+ L  +G EGLFLGGNYV GVALGRCVEGAYE A EV  F+S+  YK
Sbjct: 483 FLVGHFDILDTAKSSLTSSGYEGLFLGGNYVAGVALGRCVEGAYETAIEVNNFMSRYAYK 537

BLAST of CSPI05G02460 vs. Swiss-Prot
Match: PPOC_TOBAC (Protoporphyrinogen oxidase, chloroplastic OS=Nicotiana tabacum GN=PPXI PE=2 SV=1)

HSP 1 Score: 787.3 bits (2032), Expect = 1.1e-226
Identity = 382/503 (75.94%), Postives = 435/503 (86.48%), Query Frame = 1

Query: 47  RCSIAEGSTALSPSNASSQSSILDCVVVGAGISGLCIAQALATKHPDVAPNIIVTEAKDR 106
           RCS+A+  T  S +     ++ LDCV+VGAGISGLCIAQ ++  +P    N++VTEA+DR
Sbjct: 50  RCSVAKDYTVPSSAVDGGPAAELDCVIVGAGISGLCIAQVMSANYP----NLMVTEARDR 109

Query: 107 VGGNITTVERDGYLWEEGPNSFQPSDPILTMVVDSGLKDDLVLGDPDAPRFVLWNGKLRP 166
            GGNITTVERDGYLWEEGPNSFQPSDP+LTM VD GLKDDLVLGDP+APRFVLW GKLRP
Sbjct: 110 AGGNITTVERDGYLWEEGPNSFQPSDPMLTMAVDCGLKDDLVLGDPNAPRFVLWKGKLRP 169

Query: 167 VPAKPNDLPFFDLMSIGGKIRAGFGALGIRPPPPGREESVEEFVRRNLGNEVFERLIEPF 226
           VP+K  DL FFDLMSI GK+RAGFGA+G+RP PPG EESVE+FVRRNLG EVFERLIEPF
Sbjct: 170 VPSKLTDLAFFDLMSIPGKLRAGFGAIGLRPSPPGHEESVEQFVRRNLGGEVFERLIEPF 229

Query: 227 CSGVYAGDPSKLSMKAAFGKVWRLEQNGGSIIGGTFKALQERNKTTKPPRDPRLPKPKGQ 286
           CSGVYAGDPSKLSMKAAFGKVW+LE+ GGSIIGGTFKA++ER+ T K PRDPRLPKPKGQ
Sbjct: 230 CSGVYAGDPSKLSMKAAFGKVWKLEETGGSIIGGTFKAIKERSSTPKAPRDPRLPKPKGQ 289

Query: 287 TVGSFRKGLTMLPNAISTCLGSKVKVSWKLSSISKVDDGGYSLTYETPEGLVSILSRSVI 346
           TVGSFRKGL MLP+AIS  LGSK+K+SWKLSSI+K + GGY LTYETPEG+VS+ SRS++
Sbjct: 290 TVGSFRKGLRMLPDAISARLGSKLKLSWKLSSITKSEKGGYHLTYETPEGVVSLQSRSIV 349

Query: 347 MTVPSYIAGTLLRPISGKAADALSKFYYPPVASVTISYPKGAIRKECLIDGELKGFGQLH 406
           MTVPSY+A  +LRP+S  AADALS FYYPPV +VTI+YP+ AIR E L+DGELKGFGQLH
Sbjct: 350 MTVPSYVASNILRPLSVAAADALSNFYYPPVGAVTITYPQEAIRDERLVDGELKGFGQLH 409

Query: 407 PRSQGVTTLGTIYSSSLFPNRAPDGRVLLLNYIGGATNTGILSQTESELIEVVDRDLRKI 466
           PR+QGV TLGTIYSSSLFPNRAP GRVLLLNYIGGA N  ILS+TES+L+EVVDRDLRK+
Sbjct: 410 PRTQGVETLGTIYSSSLFPNRAPKGRVLLLNYIGGAKNPEILSKTESQLVEVVDRDLRKM 469

Query: 467 LINPNAEDPLPLSVRVWPQAIPQFLIGHLDVLDTAKAGLREAGMEGLFLGGNYVCGVALG 526
           LI P A+DPL + VRVWPQAIPQFL+GHLD L TAKA + + G+EGLFLGGNYV GVALG
Sbjct: 470 LIKPKAQDPLVVGVRVWPQAIPQFLVGHLDTLSTAKAAMNDNGLEGLFLGGNYVSGVALG 529

Query: 527 RCVEGAYEAAAEVAGFLSKKVYK 550
           RCVEGAYE A+EV GFLS+  YK
Sbjct: 530 RCVEGAYEVASEVTGFLSRYAYK 548

BLAST of CSPI05G02460 vs. Swiss-Prot
Match: PPOC_ORYSJ (Protoporphyrinogen oxidase, chloroplastic OS=Oryza sativa subsp. japonica GN=PPOX1 PE=2 SV=1)

HSP 1 Score: 785.8 bits (2028), Expect = 3.1e-226
Identity = 388/522 (74.33%), Postives = 447/522 (85.63%), Query Frame = 1

Query: 32  PLHI--SLQNNRLRSYFRCSIAEGSTALSPSNASSQSSILDCVVVGAGISGLCIAQALAT 91
           PL I  + +  R R + RC++A G+ A +P+   ++ S  DCVVVG GISGLC AQALAT
Sbjct: 19  PLRIRDAARRTRRRGHVRCAVASGA-AEAPAAPGARVSA-DCVVVGGGISGLCTAQALAT 78

Query: 92  KHPDVAPNIIVTEAKDRVGGNITTVER--DGYLWEEGPNSFQPSDPILTMVVDSGLKDDL 151
           KH     +++VTEA+ R GGNITT ER  +GYLWEEGPNSFQPSDP+LTM VDSGLKDDL
Sbjct: 79  KHG--VGDVLVTEARARPGGNITTAERAGEGYLWEEGPNSFQPSDPVLTMAVDSGLKDDL 138

Query: 152 VLGDPDAPRFVLWNGKLRPVPAKPNDLPFFDLMSIGGKIRAGFGALGIRPPPPGREESVE 211
           V GDP+APRFVLW GKLRPVP+KP DLPFFDLMSI GK+RAG GALG+R PPPGREESVE
Sbjct: 139 VFGDPNAPRFVLWEGKLRPVPSKPGDLPFFDLMSIPGKLRAGLGALGVRAPPPGREESVE 198

Query: 212 EFVRRNLGNEVFERLIEPFCSGVYAGDPSKLSMKAAFGKVWRLEQNGGSIIGGTFKALQE 271
           +FVRRNLG EVFERLIEPFCSGVYAGDPSKLSMKAAFGKVWRLE  GGSIIGGT K +QE
Sbjct: 199 DFVRRNLGAEVFERLIEPFCSGVYAGDPSKLSMKAAFGKVWRLEDTGGSIIGGTIKTIQE 258

Query: 272 RNKTTKPPRDPRLPKPKGQTVGSFRKGLTMLPNAISTCLGSKVKVSWKLSSISKVDDGGY 331
           R K  KPPRDPRLP PKGQTV SFRKGLTMLP+AI++ LGSKVK+SWKL+SI+K D+ GY
Sbjct: 259 RGKNPKPPRDPRLPTPKGQTVASFRKGLTMLPDAITSRLGSKVKLSWKLTSITKSDNKGY 318

Query: 332 SLTYETPEGLVSILSRSVIMTVPSYIAGTLLRPISGKAADALSKFYYPPVASVTISYPKG 391
           +L YETPEG+VS+ +++V+MT+PSY+A  +LRP+S  AADALS FYYPPVA+VT+SYPK 
Sbjct: 319 ALVYETPEGVVSVQAKTVVMTIPSYVASDILRPLSSDAADALSIFYYPPVAAVTVSYPKE 378

Query: 392 AIRKECLIDGELKGFGQLHPRSQGVTTLGTIYSSSLFPNRAPDGRVLLLNYIGGATNTGI 451
           AIRKECLIDGEL+GFGQLHPRSQGV TLGTIYSSSLFPNRAP GRVLLLNYIGG+TNTGI
Sbjct: 379 AIRKECLIDGELQGFGQLHPRSQGVETLGTIYSSSLFPNRAPAGRVLLLNYIGGSTNTGI 438

Query: 452 LSQTESELIEVVDRDLRKILINPNAEDPLPLSVRVWPQAIPQFLIGHLDVLDTAKAGLRE 511
           +S+TESEL+E VDRDLRK+LINP A DPL L VRVWPQAIPQFLIGHLD L+ AK+ L +
Sbjct: 439 VSKTESELVEAVDRDLRKMLINPKAVDPLVLGVRVWPQAIPQFLIGHLDHLEAAKSALGK 498

Query: 512 AGMEGLFLGGNYVCGVALGRCVEGAYEAAAEVAGFLSKKVYK 550
            G +GLFLGGNYV GVALGRCVEGAYE+A++++ +L+K  YK
Sbjct: 499 GGYDGLFLGGNYVAGVALGRCVEGAYESASQISDYLTKYAYK 536

BLAST of CSPI05G02460 vs. Swiss-Prot
Match: PPOX_MYXXA (Protoporphyrinogen oxidase OS=Myxococcus xanthus GN=hemY PE=1 SV=1)

HSP 1 Score: 214.9 bits (546), Expect = 2.2e-54
Identity = 153/476 (32.14%), Postives = 238/476 (50.00%), Query Frame = 1

Query: 69  LDCVVVGAGISGLCIAQALATKHPDVAPNIIVTEAKDRVGGNITTVERDGYLWEEGPNSF 128
           ++  VVG GISGL +A  L ++  D     ++ E+  R+GG + T    GYL E+GPNSF
Sbjct: 10  MNVAVVGGGISGLAVAHHLRSRGTDA----VLLESSARLGGAVGTHALAGYLVEQGPNSF 69

Query: 129 QPSDPILTMVVDS-GLKDDLVLGDPDAPR-FVLWNGKLRPVPAKPNDLPFFDLMSIGGKI 188
              +P    +  +  L+  +   DP A R +V   G+LR VPA P      D++ +G ++
Sbjct: 70  LDREPATRALAAALNLEGRIRAADPAAKRRYVYTRGRLRSVPASPPAFLASDILPLGARL 129

Query: 189 RAGFGALGIRPPPPGREESVEEFVRRNLGNEVFERLIEPFCSGVYAGDPSKLSMKAAFGK 248
           R   G L  R  P G +ES+  F RR+LG+   + L++   +G+YAGD  +LS+ A F  
Sbjct: 130 RVA-GELFSRRAPEGVDESLAAFGRRHLGHRATQVLLDAVQTGIYAGDVEQLSVAATFPM 189

Query: 249 VWRLEQNGGSIIGGTFKALQERNKTTKPPRDPRLPKPKGQTVGSFRKGLTMLPNAISTCL 308
           + ++E+   S+I G  +A + + +   P      PK  G  + +F  GL +L +A++  L
Sbjct: 190 LVKMEREHRSLILGAIRAQKAQRQAALPA--GTAPKLSG-ALSTFDGGLQVLIDALAASL 249

Query: 309 GSKVKVSWKLSSISKVDDGGYSLTYETPEGLVSILSRSVIMTVPSYIAGTLLRPISGKAA 368
           G    V  ++  +++ +DGG+ L  E       +    V++  P++    LLRP+    A
Sbjct: 250 GDAAHVGARVEGLAR-EDGGWRLIIEEHGRRAELSVAQVVLAAPAHATAKLLRPLDDALA 309

Query: 369 DALSKFYYPPVASVTISYPKGAIRKECLIDGELKGFGQLHPRSQGVTTLGTIYSSSLFPN 428
             ++   Y P+A V + +  G +           GFG L P  +    LG I++S+ FP 
Sbjct: 310 ALVAGIAYAPIAVVHLGFDAGTLPAP-------DGFGFLVPAEEQRRMLGAIHASTTFPF 369

Query: 429 RAPDGRVLLLNYIGGATNTGILSQTESELIEVVDRDLRKILINPNAEDPLPLSVRVWPQA 488
           RA  GRVL    +GGA   G++ Q E  L  +   +L K L    A  P    V  WP  
Sbjct: 370 RAEGGRVLYSCMVGGARQPGLVEQDEDALAALAREEL-KALAGVTAR-PSFTRVFRWPLG 429

Query: 489 IPQFLIGHLDVLDTAKAGLREAGMEGLFLGGNYVCGVALGRCVEGAYEAA-AEVAG 542
           IPQ+ +GHL+ +    A L+   + GL L GN   GV L  C+  A + A A VAG
Sbjct: 430 IPQYNLGHLERVAAIDAALQR--LPGLHLIGNAYKGVGLNDCIRNAAQLADALVAG 465

BLAST of CSPI05G02460 vs. Swiss-Prot
Match: PPOCM_SPIOL (Protoporphyrinogen oxidase, chloroplastic/mitochondrial OS=Spinacia oleracea GN=POX2 PE=1 SV=1)

HSP 1 Score: 194.9 bits (494), Expect = 2.3e-48
Identity = 145/489 (29.65%), Postives = 239/489 (48.88%), Query Frame = 1

Query: 73  VVGAGISGLCIAQALATKHPDVAPNIIVTEAKDRVGGNITTVERDGYLWEEGPNSFQPSD 132
           VVGAG+SGL  A  L +       N+ + EA  R GG + TV +DG +W+EG N+   SD
Sbjct: 47  VVGAGVSGLAAAYKLKSN----GLNVTLFEADSRAGGKLKTVVKDGLIWDEGANTMTESD 106

Query: 133 PILTMVVDS-GLKDDLVLGDPDAPRFVLWNGKLRPVPAKPNDLPFFDLMSIGGKIRAGFG 192
             +T + D  G+++ L L      R++  +G    +P+ P  L   +++S   K++    
Sbjct: 107 EEVTSLFDDLGIREKLQLPISQNKRYIARDGLPVLLPSNPVALLKSNILSAKSKLQIMLE 166

Query: 193 AL------GIRPPPPGREESVEEFVRRNLGNEVFERLIEPFCSGVYAGDPSKLSMKAAFG 252
                   G +      +ESV EF  R+ G E  + LI+PF +G   GDP  LSM+ AF 
Sbjct: 167 PFLWKKHNGAKVSDENAQESVAEFFERHFGKEFVDYLIDPFVAGTSGGDPQSLSMRHAFP 226

Query: 253 KVWRLEQNGGSIIGGTFKALQERNKTTKPPRDPRLPKPKGQTVGSFRKGLTMLPNAISTC 312
           ++W +E   GS+I G  ++     K     +     KP+ +   SF+ G+  L + I   
Sbjct: 227 ELWNIENRFGSVISGFIQSKLSSKKEKGGEKQSSNKKPRVRGSFSFQGGMQTLVDTICKE 286

Query: 313 LG-SKVKVSWKLSSISKVDDGGYSLTYETPEGLVSILSRSVIMTVPSYIAGTLLRPISG- 372
            G  ++K+  ++ S+S   +G  SLT E     VS +S S I   P Y A  +  PI+  
Sbjct: 287 FGEDELKLQSEVLSLSYSHNG--SLTSE--NWSVSSMSNSTIQDQP-YDAVVVTAPINNV 346

Query: 373 ------KAADALSKFYYPPVASVTISYPKGAIRKECLIDGELKGFGQLHPRSQ---GVTT 432
                 K  +  S  + P V+ + +S      +K   +   L+GFG L P ++   G+ T
Sbjct: 347 KELKIMKVENPFSLDFIPEVSCLPLSVIITTFKK-TNVKRPLEGFGVLVPSNEQHNGLKT 406

Query: 433 LGTIYSSSLFPNRAPDGRVLLLNYIGGATNTGILSQTESELIEVVDRDLRKILINPNAED 492
           LGT++SS +FP+RAP    L   ++GG+ N  +   +  EL ++V  DL+++L      +
Sbjct: 407 LGTLFSSMMFPDRAPSDVYLYTTFVGGSRNRELAKASTDELKQIVSSDLQQLL--GTEGE 466

Query: 493 PLPLSVRVWPQAIPQFLIGHLDVLDTAKAGLREAGMEGLFLGGNYVCGVALGRCVEGAYE 544
           P  ++   W +A P +   +  VL   +    E  + GLF  GN+  G+++G+ +   Y+
Sbjct: 467 PTFVNHFYWSKAFPLYGRNYDSVLRAIEK--MERDLPGLFYAGNHKGGLSVGKSIASGYK 521

BLAST of CSPI05G02460 vs. TrEMBL
Match: A0A0A0KMZ2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G128250 PE=4 SV=1)

HSP 1 Score: 1104.0 bits (2854), Expect = 0.0e+00
Identity = 549/549 (100.00%), Postives = 549/549 (100.00%), Query Frame = 1

Query: 1   MATGSTLLTDLPFRRPHPLTLLRPSNIPSFHPLHISLQNNRLRSYFRCSIAEGSTALSPS 60
           MATGSTLLTDLPFRRPHPLTLLRPSNIPSFHPLHISLQNNRLRSYFRCSIAEGSTALSPS
Sbjct: 1   MATGSTLLTDLPFRRPHPLTLLRPSNIPSFHPLHISLQNNRLRSYFRCSIAEGSTALSPS 60

Query: 61  NASSQSSILDCVVVGAGISGLCIAQALATKHPDVAPNIIVTEAKDRVGGNITTVERDGYL 120
           NASSQSSILDCVVVGAGISGLCIAQALATKHPDVAPNIIVTEAKDRVGGNITTVERDGYL
Sbjct: 61  NASSQSSILDCVVVGAGISGLCIAQALATKHPDVAPNIIVTEAKDRVGGNITTVERDGYL 120

Query: 121 WEEGPNSFQPSDPILTMVVDSGLKDDLVLGDPDAPRFVLWNGKLRPVPAKPNDLPFFDLM 180
           WEEGPNSFQPSDPILTMVVDSGLKDDLVLGDPDAPRFVLWNGKLRPVPAKPNDLPFFDLM
Sbjct: 121 WEEGPNSFQPSDPILTMVVDSGLKDDLVLGDPDAPRFVLWNGKLRPVPAKPNDLPFFDLM 180

Query: 181 SIGGKIRAGFGALGIRPPPPGREESVEEFVRRNLGNEVFERLIEPFCSGVYAGDPSKLSM 240
           SIGGKIRAGFGALGIRPPPPGREESVEEFVRRNLGNEVFERLIEPFCSGVYAGDPSKLSM
Sbjct: 181 SIGGKIRAGFGALGIRPPPPGREESVEEFVRRNLGNEVFERLIEPFCSGVYAGDPSKLSM 240

Query: 241 KAAFGKVWRLEQNGGSIIGGTFKALQERNKTTKPPRDPRLPKPKGQTVGSFRKGLTMLPN 300
           KAAFGKVWRLEQNGGSIIGGTFKALQERNKTTKPPRDPRLPKPKGQTVGSFRKGLTMLPN
Sbjct: 241 KAAFGKVWRLEQNGGSIIGGTFKALQERNKTTKPPRDPRLPKPKGQTVGSFRKGLTMLPN 300

Query: 301 AISTCLGSKVKVSWKLSSISKVDDGGYSLTYETPEGLVSILSRSVIMTVPSYIAGTLLRP 360
           AISTCLGSKVKVSWKLSSISKVDDGGYSLTYETPEGLVSILSRSVIMTVPSYIAGTLLRP
Sbjct: 301 AISTCLGSKVKVSWKLSSISKVDDGGYSLTYETPEGLVSILSRSVIMTVPSYIAGTLLRP 360

Query: 361 ISGKAADALSKFYYPPVASVTISYPKGAIRKECLIDGELKGFGQLHPRSQGVTTLGTIYS 420
           ISGKAADALSKFYYPPVASVTISYPKGAIRKECLIDGELKGFGQLHPRSQGVTTLGTIYS
Sbjct: 361 ISGKAADALSKFYYPPVASVTISYPKGAIRKECLIDGELKGFGQLHPRSQGVTTLGTIYS 420

Query: 421 SSLFPNRAPDGRVLLLNYIGGATNTGILSQTESELIEVVDRDLRKILINPNAEDPLPLSV 480
           SSLFPNRAPDGRVLLLNYIGGATNTGILSQTESELIEVVDRDLRKILINPNAEDPLPLSV
Sbjct: 421 SSLFPNRAPDGRVLLLNYIGGATNTGILSQTESELIEVVDRDLRKILINPNAEDPLPLSV 480

Query: 481 RVWPQAIPQFLIGHLDVLDTAKAGLREAGMEGLFLGGNYVCGVALGRCVEGAYEAAAEVA 540
           RVWPQAIPQFLIGHLDVLDTAKAGLREAGMEGLFLGGNYVCGVALGRCVEGAYEAAAEVA
Sbjct: 481 RVWPQAIPQFLIGHLDVLDTAKAGLREAGMEGLFLGGNYVCGVALGRCVEGAYEAAAEVA 540

Query: 541 GFLSKKVYK 550
           GFLSKKVYK
Sbjct: 541 GFLSKKVYK 549

BLAST of CSPI05G02460 vs. TrEMBL
Match: A0A072VNG3_MEDTR (Protoporphyrinogen IX oxidase OS=Medicago truncatula GN=MTR_1g085730 PE=4 SV=1)

HSP 1 Score: 860.9 bits (2223), Expect = 8.4e-247
Identity = 434/545 (79.63%), Postives = 472/545 (86.61%), Query Frame = 1

Query: 16  PHPLTLLRP---SNIPSFHPLHISLQNNRLRS---YFRCSIAEGSTALSPSNASS----- 75
           P   TLLRP   S  PS +     ++N    +     RCSIAE STA SP+  +S     
Sbjct: 8   PPTQTLLRPYLHSPFPSPNSKSKFIRNRNHHNPILRLRCSIAEESTA-SPTKTTSKSNSG 67

Query: 76  QSSILDCVVVGAGISGLCIAQALATKHPDVAPNIIVTEAKDRVGGNITTVERDGYLWEEG 135
           +SS +DCVVVG GISGLCIAQAL+TKH    PN++VTEAKDRVGGNI TVERDG+LWEEG
Sbjct: 68  ESSSVDCVVVGGGISGLCIAQALSTKHAHGVPNVVVTEAKDRVGGNIITVERDGFLWEEG 127

Query: 136 PNSFQPSDPILTMVVDSGLKDDLVLGDPDAPRFVLWNGKLRPVPAKPNDLPFFDLMSIGG 195
           PNSFQPSDP+LTMVVDSGLKDDLVLGDPDAPRFVLWNGKLRPVP KP DLPFFDLMSIGG
Sbjct: 128 PNSFQPSDPMLTMVVDSGLKDDLVLGDPDAPRFVLWNGKLRPVPGKPTDLPFFDLMSIGG 187

Query: 196 KIRAGFGALGIRPPPPGREESVEEFVRRNLGNEVFERLIEPFCSGVYAGDPSKLSMKAAF 255
           KIRAGFGALGIRPPPPG EESVEEFVRRNLG EVFERLIEPFCSGVYAGDPSKLSMKAAF
Sbjct: 188 KIRAGFGALGIRPPPPGHEESVEEFVRRNLGAEVFERLIEPFCSGVYAGDPSKLSMKAAF 247

Query: 256 GKVWRLEQNGGSIIGGTFKALQERNKTTKPPRDPRLPKPKGQTVGSFRKGLTMLPNAIST 315
           GKVWRLEQNGGSIIGG FKA+QERNK +KPPRDPRLPKPKGQTVGSFRKGLTMLPNAIS 
Sbjct: 248 GKVWRLEQNGGSIIGGAFKAIQERNKVSKPPRDPRLPKPKGQTVGSFRKGLTMLPNAISA 307

Query: 316 CLGSKVKVSWKLSSISKVDDGGYSLTYETPEGLVSILSRSVIMTVPSYIAGTLLRPISGK 375
            LG+KVK+SWKL SISK+D G YSLTYETPEG+VS+ S++VIMT+PS++A  LLRP+S  
Sbjct: 308 RLGNKVKLSWKLLSISKLDSGEYSLTYETPEGVVSLQSKTVIMTIPSHVASPLLRPLSPA 367

Query: 376 AADALSKFYYPPVASVTISYPKGAIRKECLIDGELKGFGQLHPRSQGVTTLGTIYSSSLF 435
           AADALSKFYYPPVA+V+ISYPK AIR ECLIDGELKGFGQLHPRSQGV TLGTIYSSSLF
Sbjct: 368 AADALSKFYYPPVAAVSISYPKEAIRSECLIDGELKGFGQLHPRSQGVQTLGTIYSSSLF 427

Query: 436 PNRAPDGRVLLLNYIGGATNTGILSQTESELIEVVDRDLRKILINPNAEDPLPLSVRVWP 495
           PNRAP GRVLLLNYIGGATN+GILS+TE EL+E VDRDLRKILINPNA+DP+ L VRVWP
Sbjct: 428 PNRAPPGRVLLLNYIGGATNSGILSKTEGELVEAVDRDLRKILINPNAQDPIVLGVRVWP 487

Query: 496 QAIPQFLIGHLDVLDTAKAGLREAGMEGLFLGGNYVCGVALGRCVEGAYEAAAEVAGFLS 550
           QAIPQFL+GHLD+LD AK  L+  G EGLFLGGNYV GVALGRCVEGAYE AAEV  FLS
Sbjct: 488 QAIPQFLVGHLDLLDAAKVSLKNTGFEGLFLGGNYVSGVALGRCVEGAYEIAAEVNDFLS 547

BLAST of CSPI05G02460 vs. TrEMBL
Match: A0A0D2SID4_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_007G117800 PE=4 SV=1)

HSP 1 Score: 852.4 bits (2201), Expect = 3.0e-244
Identity = 422/535 (78.88%), Postives = 465/535 (86.92%), Query Frame = 1

Query: 19  LTLLRPSNIPSFHPLHISLQNN--RLRSYF--RCSIAEGSTALSPSNASSQSSILDCVVV 78
           L+LLR S  PS  P  I    +  R R  F  RCS+AEG T  S      +SSI DCV+V
Sbjct: 7   LSLLRSS--PSVSPFSIPHHQHPPRFRKPFKLRCSLAEGPTISSSKIDGGESSIADCVIV 66

Query: 79  GAGISGLCIAQALATKHPDVAPNIIVTEAKDRVGGNITTVERDGYLWEEGPNSFQPSDPI 138
           G GISGLCIAQALATKH DVA N+IVTEA+DRVGGNITTVERDGYLWEEGPNSFQPSDPI
Sbjct: 67  GGGISGLCIAQALATKHRDVASNVIVTEARDRVGGNITTVERDGYLWEEGPNSFQPSDPI 126

Query: 139 LTMVVDSGLKDDLVLGDPDAPRFVLWNGKLRPVPAKPNDLPFFDLMSIGGKIRAGFGALG 198
           LTM VDSGLKDDLVLGDP+APRFVLW GKLRPVP+KP DLPFFDLMSI GK+RAGFGA+G
Sbjct: 127 LTMAVDSGLKDDLVLGDPNAPRFVLWEGKLRPVPSKPTDLPFFDLMSIAGKLRAGFGAIG 186

Query: 199 IRPPPPGREESVEEFVRRNLGNEVFERLIEPFCSGVYAGDPSKLSMKAAFGKVWRLEQNG 258
           IRPPPPG EESVEEFVRRNLG EVFER IEPFCSGVYAGDPSKLSMKAAFG+VW+LE+ G
Sbjct: 187 IRPPPPGYEESVEEFVRRNLGAEVFERFIEPFCSGVYAGDPSKLSMKAAFGRVWKLEEIG 246

Query: 259 GSIIGGTFKALQERNKTTKPPRDPRLPKPKGQTVGSFRKGLTMLPNAISTCLGSKVKVSW 318
           GSIIGGTFK +QERNKT KPPRDPRLPKPKGQTVGSFRKGLTMLP AI+  LGS VK+SW
Sbjct: 247 GSIIGGTFKTIQERNKTPKPPRDPRLPKPKGQTVGSFRKGLTMLPEAIANSLGSNVKLSW 306

Query: 319 KLSSISKVDDGGYSLTYETPEGLVSILSRSVIMTVPSYIAGTLLRPISGKAADALSKFYY 378
           KLSSI+K+ +GGY+L +ETPEG+VS+ SRSV+MT+PS++A  LL P+S  AADALS+FYY
Sbjct: 307 KLSSITKLGNGGYNLQFETPEGMVSLQSRSVVMTIPSHVASNLLHPLSAAAADALSQFYY 366

Query: 379 PPVASVTISYPKGAIRKECLIDGELKGFGQLHPRSQGVTTLGTIYSSSLFPNRAPDGRVL 438
           PPVASVT+SYPK AIRKECLIDGELKGFGQLHPRSQG+ TLGTIYSSSLFPNRAP GRVL
Sbjct: 367 PPVASVTVSYPKEAIRKECLIDGELKGFGQLHPRSQGIETLGTIYSSSLFPNRAPSGRVL 426

Query: 439 LLNYIGGATNTGILSQTESELIEVVDRDLRKILINPNAEDPLPLSVRVWPQAIPQFLIGH 498
           LLNYIGGATNTGILS+TE EL+E VDRDLRK+LINPNA+DPL L VRVWP+AIPQFL+GH
Sbjct: 427 LLNYIGGATNTGILSKTEGELVEAVDRDLRKMLINPNAKDPLVLGVRVWPKAIPQFLVGH 486

Query: 499 LDVLDTAKAGLREAGMEGLFLGGNYVCGVALGRCVEGAYEAAAEVAGFLSKKVYK 550
           LD+LD+AK  LR++G  GLFLGGNYV GVALGRCVEGAYE AAEV  FLS+  YK
Sbjct: 487 LDLLDSAKMALRDSGFHGLFLGGNYVSGVALGRCVEGAYEVAAEVKEFLSQYAYK 539

BLAST of CSPI05G02460 vs. TrEMBL
Match: A0A151SSA5_CAJCA (Uncharacterized protein OS=Cajanus cajan GN=KK1_003887 PE=4 SV=1)

HSP 1 Score: 852.0 bits (2200), Expect = 3.9e-244
Identity = 424/550 (77.09%), Postives = 476/550 (86.55%), Query Frame = 1

Query: 5   STLLTDLPFRRPHPLTLLRPS-----NIPSFHPLHISLQNNRLRSYFRCSIAEGSTALSP 64
           +T+ +D+ F  P   TLLRPS     ++ S  P        R     RCSI + S A+SP
Sbjct: 2   ATVFSDILF--PPNQTLLRPSLHSPTSLFSSSPTR-KFPRTRPNPVLRCSITKES-AVSP 61

Query: 65  SNASSQSSILDCVVVGAGISGLCIAQALATKHPDVAPNIIVTEAKDRVGGNITTVERDGY 124
             A   + + DCVVVG GISGLCIAQAL+TKH   + N++VTEA+DRVGGNITT+ERDGY
Sbjct: 62  PKARDAARV-DCVVVGGGISGLCIAQALSTKHASTSANVVVTEARDRVGGNITTLERDGY 121

Query: 125 LWEEGPNSFQPSDPILTMVVDSGLKDDLVLGDPDAPRFVLWNGKLRPVPAKPNDLPFFDL 184
           LWEEGPNSFQPSDP+LTMVVDSGLKD+LVLGDP+APRFVLWNGKLRPVP KP DLPFFDL
Sbjct: 122 LWEEGPNSFQPSDPMLTMVVDSGLKDELVLGDPNAPRFVLWNGKLRPVPGKPTDLPFFDL 181

Query: 185 MSIGGKIRAGFGALGIRPPPPGREESVEEFVRRNLGNEVFERLIEPFCSGVYAGDPSKLS 244
           MS+GGKIRAGFGALGIRPPPPG EESVEEFVRRNLG+EVFERLIEPFCSGVYAGDPSKLS
Sbjct: 182 MSLGGKIRAGFGALGIRPPPPGHEESVEEFVRRNLGDEVFERLIEPFCSGVYAGDPSKLS 241

Query: 245 MKAAFGKVWRLEQNGGSIIGGTFKALQERNKTTKPPRDPRLPKPKGQTVGSFRKGLTMLP 304
           MKAAFGKVWRLEQNGGSIIGGTFKA+QERN T+KPPRDPRLPKPKGQTVGSFRKGLTMLP
Sbjct: 242 MKAAFGKVWRLEQNGGSIIGGTFKAIQERNATSKPPRDPRLPKPKGQTVGSFRKGLTMLP 301

Query: 305 NAISTCLGSKVKVSWKLSSISKVDDGGYSLTYETPEGLVSILSRSVIMTVPSYIAGTLLR 364
           +AIS  LG+KVK+SWKLSSISK+D G Y+LTYETPEG+VS+  ++V++T+PS++A TLLR
Sbjct: 302 DAISARLGNKVKLSWKLSSISKLDSGEYNLTYETPEGVVSLQCKTVVLTIPSHVASTLLR 361

Query: 365 PISGKAADALSKFYYPPVASVTISYPKGAIRKECLIDGELKGFGQLHPRSQGVTTLGTIY 424
           P+S  AADALSKFYYPPVA+V+ISYPK A+R ECLIDGELKGFGQLHPRSQGV TLGTIY
Sbjct: 362 PLSAAAADALSKFYYPPVAAVSISYPKEALRSECLIDGELKGFGQLHPRSQGVETLGTIY 421

Query: 425 SSSLFPNRAPDGRVLLLNYIGGATNTGILSQTESELIEVVDRDLRKILINPNAEDPLPLS 484
           SSSLFPNRAP GRVLLLNYIGGATNTGILS+T+SEL+E VDRDLRKILINPNA+DP  L 
Sbjct: 422 SSSLFPNRAPPGRVLLLNYIGGATNTGILSKTDSELVEAVDRDLRKILINPNAQDPFVLG 481

Query: 485 VRVWPQAIPQFLIGHLDVLDTAKAGLREAGMEGLFLGGNYVCGVALGRCVEGAYEAAAEV 544
           VR+WPQAIPQFL+GHLD+LD AK+ L+  G EGLFLGGNYV GVALGRCVEGAYE AAEV
Sbjct: 482 VRLWPQAIPQFLVGHLDLLDVAKSSLKNTGFEGLFLGGNYVSGVALGRCVEGAYELAAEV 541

Query: 545 AGFLSKKVYK 550
             FL+ +VYK
Sbjct: 542 NDFLTNRVYK 546

BLAST of CSPI05G02460 vs. TrEMBL
Match: A0A061DRW0_THECC (Flavin containing amine oxidoreductase family OS=Theobroma cacao GN=TCM_004957 PE=4 SV=1)

HSP 1 Score: 851.3 bits (2198), Expect = 6.6e-244
Identity = 424/544 (77.94%), Postives = 463/544 (85.11%), Query Frame = 1

Query: 6   TLLTDLPFRRPHPLTLLRPSNIPSFHPLHISLQNNRLRSYFRCSIAEGSTALSPSNASSQ 65
           T +TD+   R  P     P +IP    LH   +  +LR    CSIAEG T  S      +
Sbjct: 2   TAVTDISLLRSSPFVF--PFSIPRHQILHRVYKPFKLR----CSIAEGPTISSSKIEGGE 61

Query: 66  SSILDCVVVGAGISGLCIAQALATKHPDVAPNIIVTEAKDRVGGNITTVERDGYLWEEGP 125
           S+I DCV+VGAGISGLCIAQALATKH DVA N+IVTEA+DRVGGNITTVERDGYLWEEGP
Sbjct: 62  STIADCVIVGAGISGLCIAQALATKHRDVASNVIVTEARDRVGGNITTVERDGYLWEEGP 121

Query: 126 NSFQPSDPILTMVVDSGLKDDLVLGDPDAPRFVLWNGKLRPVPAKPNDLPFFDLMSIGGK 185
           NSFQPSDPILTM VDSGLKDDLVLGDPDAPRFVLW GKLRPVP+KP DLP FDLMSIGGK
Sbjct: 122 NSFQPSDPILTMAVDSGLKDDLVLGDPDAPRFVLWEGKLRPVPSKPTDLPVFDLMSIGGK 181

Query: 186 IRAGFGALGIRPPPPGREESVEEFVRRNLGNEVFERLIEPFCSGVYAGDPSKLSMKAAFG 245
           +RAG GALGIRPPPPG EESVEEFVRRNLG EVFERLIEPFCSGVYAGDPSKLSMKAAFG
Sbjct: 182 LRAGLGALGIRPPPPGHEESVEEFVRRNLGAEVFERLIEPFCSGVYAGDPSKLSMKAAFG 241

Query: 246 KVWRLEQNGGSIIGGTFKALQERNKTTKPPRDPRLPKPKGQTVGSFRKGLTMLPNAISTC 305
           +VW+LEQ GGSII GTFK +QERNKT KPPRDPRLPKPKGQTVGSFRKGL MLP AI+T 
Sbjct: 242 RVWKLEQIGGSIISGTFKTIQERNKTPKPPRDPRLPKPKGQTVGSFRKGLAMLPEAIATS 301

Query: 306 LGSKVKVSWKLSSISKVDDGGYSLTYETPEGLVSILSRSVIMTVPSYIAGTLLRPISGKA 365
           LG KVK+SWKLSSI+K+++GGYSLTYETPEG+VS+ SRSV+MTVPS++A  LL P+S  A
Sbjct: 302 LGGKVKLSWKLSSITKLENGGYSLTYETPEGMVSLQSRSVVMTVPSHVASNLLHPLSAAA 361

Query: 366 ADALSKFYYPPVASVTISYPKGAIRKECLIDGELKGFGQLHPRSQGVTTLGTIYSSSLFP 425
           ADALS+FYYPPVASVT+SYPK AIR+ECLIDGELKGFGQLHPRSQG+ TLGTIYSSSLF 
Sbjct: 362 ADALSQFYYPPVASVTVSYPKEAIRRECLIDGELKGFGQLHPRSQGIETLGTIYSSSLFR 421

Query: 426 NRAPDGRVLLLNYIGGATNTGILSQTESELIEVVDRDLRKILINPNAEDPLPLSVRVWPQ 485
           NRAP GRVLLLNYIGGATN GILS+TE EL+E VDRDLRK+LINPNA+D   L VRVWPQ
Sbjct: 422 NRAPSGRVLLLNYIGGATNPGILSKTEGELVEAVDRDLRKMLINPNAKDSHILGVRVWPQ 481

Query: 486 AIPQFLIGHLDVLDTAKAGLREAGMEGLFLGGNYVCGVALGRCVEGAYEAAAEVAGFLSK 545
           AIPQFL+GHLD+LDTAK  L  +G  GLFLGGNYV GVALGRCVEGAYE AAEV  FLS+
Sbjct: 482 AIPQFLVGHLDLLDTAKTALMNSGFHGLFLGGNYVSGVALGRCVEGAYEVAAEVKDFLSQ 539

Query: 546 KVYK 550
             YK
Sbjct: 542 YAYK 539

BLAST of CSPI05G02460 vs. TAIR10
Match: AT4G01690.1 (AT4G01690.1 Flavin containing amine oxidoreductase family)

HSP 1 Score: 823.9 bits (2127), Expect = 5.7e-239
Identity = 412/540 (76.30%), Postives = 457/540 (84.63%), Query Frame = 1

Query: 19  LTLLRPSN---IPSFHPLHISLQNNRLRSY----FRCSIAEGSTALSPSNASSQSSIL-- 78
           L+LLRP+    +PSF     S  N RL  Y     RCS+A G T  S        + +  
Sbjct: 3   LSLLRPTTQSLLPSF-----SKPNLRLNVYKPLRLRCSVAGGPTVGSSKIEGGGGTTITT 62

Query: 79  DCVVVGAGISGLCIAQALATKHPDVAPNIIVTEAKDRVGGNITTVERDGYLWEEGPNSFQ 138
           DCV+VG GISGLCIAQALATKHPD APN+IVTEAKDRVGGNI T E +G+LWEEGPNSFQ
Sbjct: 63  DCVIVGGGISGLCIAQALATKHPDAAPNLIVTEAKDRVGGNIITREENGFLWEEGPNSFQ 122

Query: 139 PSDPILTMVVDSGLKDDLVLGDPDAPRFVLWNGKLRPVPAKPNDLPFFDLMSIGGKIRAG 198
           PSDP+LTMVVDSGLKDDLVLGDP APRFVLWNGKLRPVP+K  DLPFFDLMSIGGKIRAG
Sbjct: 123 PSDPMLTMVVDSGLKDDLVLGDPTAPRFVLWNGKLRPVPSKLTDLPFFDLMSIGGKIRAG 182

Query: 199 FGALGIRPPPPGREESVEEFVRRNLGNEVFERLIEPFCSGVYAGDPSKLSMKAAFGKVWR 258
           FGALGIRP PPGREESVEEFVRRNLG+EVFERLIEPFCSGVYAGDPSKLSMKAAFGKVW+
Sbjct: 183 FGALGIRPSPPGREESVEEFVRRNLGDEVFERLIEPFCSGVYAGDPSKLSMKAAFGKVWK 242

Query: 259 LEQNGGSIIGGTFKALQERNKTTKPPRDPRLPKPKGQTVGSFRKGLTMLPNAISTCLGSK 318
           LEQNGGSIIGGTFKA+QER    K  RDPRLPKP+GQTVGSFRKGL MLP AIS  LGSK
Sbjct: 243 LEQNGGSIIGGTFKAIQERKNAPKAERDPRLPKPQGQTVGSFRKGLRMLPEAISARLGSK 302

Query: 319 VKVSWKLSSISKVDDGGYSLTYETPEGLVSILSRSVIMTVPSYIAGTLLRPISGKAADAL 378
           VK+SWKLS I+K++ GGY+LTYETP+GLVS+ S+SV+MTVPS++A  LLRP+S  AA+AL
Sbjct: 303 VKLSWKLSGITKLESGGYNLTYETPDGLVSVQSKSVVMTVPSHVASGLLRPLSESAANAL 362

Query: 379 SKFYYPPVASVTISYPKGAIRKECLIDGELKGFGQLHPRSQGVTTLGTIYSSSLFPNRAP 438
           SK YYPPVA+V+ISYPK AIR ECLIDGELKGFGQLHPR+QGV TLGTIYSSSLFPNRAP
Sbjct: 363 SKLYYPPVAAVSISYPKEAIRTECLIDGELKGFGQLHPRTQGVETLGTIYSSSLFPNRAP 422

Query: 439 DGRVLLLNYIGGATNTGILSQTESELIEVVDRDLRKILINPNAEDPLPLSVRVWPQAIPQ 498
            GR+LLLNYIGG+TNTGILS++E EL+E VDRDLRK+LI PN+ DPL L VRVWPQAIPQ
Sbjct: 423 PGRILLLNYIGGSTNTGILSKSEGELVEAVDRDLRKMLIKPNSTDPLKLGVRVWPQAIPQ 482

Query: 499 FLIGHLDVLDTAKAGLREAGMEGLFLGGNYVCGVALGRCVEGAYEAAAEVAGFLSKKVYK 550
           FL+GH D+LDTAK+ L  +G EGLFLGGNYV GVALGRCVEGAYE A EV  F+S+  YK
Sbjct: 483 FLVGHFDILDTAKSSLTSSGYEGLFLGGNYVAGVALGRCVEGAYETAIEVNNFMSRYAYK 537

BLAST of CSPI05G02460 vs. TAIR10
Match: AT5G14220.1 (AT5G14220.1 Flavin containing amine oxidoreductase family)

HSP 1 Score: 176.8 bits (447), Expect = 3.7e-44
Identity = 141/495 (28.48%), Postives = 239/495 (48.28%), Query Frame = 1

Query: 73  VVGAGISGLCIAQALATKHPDVAPNIIVTEAKDRVGGNITTVERDGYLWEEGPNSFQPSD 132
           VVGAG+SGL  A  L ++      N+ V EA  RVGG + +V ++G +W+EG N+   ++
Sbjct: 21  VVGAGVSGLAAAYKLKSR----GLNVTVFEADGRVGGKLRSVMQNGLIWDEGANTMTEAE 80

Query: 133 P-ILTMVVDSGLKDDLVLGDPDAPRFVLWNGKLRPVPAKPNDLPFFDLMSIGGKIRAGFG 192
           P + +++ D GL++          R+++ NG    +P  P +L    ++S   K +    
Sbjct: 81  PEVGSLLDDLGLREKQQFPISQKKRYIVRNGVPVMLPTNPIELVTSSVLSTQSKFQILLE 140

Query: 193 AL-----GIRPPPPGREESVEEFVRRNLGNEVFERLIEPFCSGVYAGDPSKLSMKAAFGK 252
                    +      EESV EF +R+ G EV + LI+PF  G  A DP  LSMK +F  
Sbjct: 141 PFLWKKKSSKVSDASAEESVSEFFQRHFGQEVVDYLIDPFVGGTSAADPDSLSMKHSFPD 200

Query: 253 VWRLEQNGGSIIGGT----FKALQERNKTTKPPRDPRLPKPKGQTVG--SFRKGLTMLPN 312
           +W +E++ GSII G     F A   +++ TK       P  K  + G  SF+ G+ +LP+
Sbjct: 201 LWNVEKSFGSIIVGAIRTKFAAKGGKSRDTKSS-----PGTKKGSRGSFSFKGGMQILPD 260

Query: 313 AISTCLG-------SKVKVSWKLSSISKVDDGGYSLTYETPEGLVSILSRSVIMTVPSYI 372
            +   L        SKV +S   +S S+ ++   S          +    +VIMT P   
Sbjct: 261 TLCKSLSHDEINLDSKV-LSLSYNSGSRQENWSLSCVSHNETQRQNPHYDAVIMTAPLCN 320

Query: 373 AGTLLRPISGK--AADALSKFYYPPVASVTISYPKGAIRKECLIDGELKGFGQLHP---R 432
              +     G+    + L +  Y P++ +  ++ K  +++       L+GFG L P   +
Sbjct: 321 VKEMKVMKGGQPFQLNFLPEINYMPLSVLITTFTKEKVKR------PLEGFGVLIPSKEQ 380

Query: 433 SQGVTTLGTIYSSSLFPNRAPDGRVLLLNYIGGATNTGILSQTESELIEVVDRDLRKILI 492
             G  TLGT++SS +FP+R+P    L   +IGG+ N  +   +  EL +VV  DL+++L 
Sbjct: 381 KHGFKTLGTLFSSMMFPDRSPSDVHLYTTFIGGSRNQELAKASTDELKQVVTSDLQRLL- 440

Query: 493 NPNAEDPLPLSVRVWPQAIPQFLIGHLDVLDTAKAGLREAGMEGLFLGGNYVCGVALGRC 544
                +P+ ++   W +A P +   +  V++       E  + G F  GN+  G+++G+ 
Sbjct: 441 -GVEGEPVSVNHYYWRKAFPLYDSSYDSVMEAIDK--MENDLPGFFYAGNHRGGLSVGKS 495

BLAST of CSPI05G02460 vs. NCBI nr
Match: gi|449463515|ref|XP_004149479.1| (PREDICTED: protoporphyrinogen oxidase 1, chloroplastic [Cucumis sativus])

HSP 1 Score: 1104.0 bits (2854), Expect = 0.0e+00
Identity = 549/549 (100.00%), Postives = 549/549 (100.00%), Query Frame = 1

Query: 1   MATGSTLLTDLPFRRPHPLTLLRPSNIPSFHPLHISLQNNRLRSYFRCSIAEGSTALSPS 60
           MATGSTLLTDLPFRRPHPLTLLRPSNIPSFHPLHISLQNNRLRSYFRCSIAEGSTALSPS
Sbjct: 1   MATGSTLLTDLPFRRPHPLTLLRPSNIPSFHPLHISLQNNRLRSYFRCSIAEGSTALSPS 60

Query: 61  NASSQSSILDCVVVGAGISGLCIAQALATKHPDVAPNIIVTEAKDRVGGNITTVERDGYL 120
           NASSQSSILDCVVVGAGISGLCIAQALATKHPDVAPNIIVTEAKDRVGGNITTVERDGYL
Sbjct: 61  NASSQSSILDCVVVGAGISGLCIAQALATKHPDVAPNIIVTEAKDRVGGNITTVERDGYL 120

Query: 121 WEEGPNSFQPSDPILTMVVDSGLKDDLVLGDPDAPRFVLWNGKLRPVPAKPNDLPFFDLM 180
           WEEGPNSFQPSDPILTMVVDSGLKDDLVLGDPDAPRFVLWNGKLRPVPAKPNDLPFFDLM
Sbjct: 121 WEEGPNSFQPSDPILTMVVDSGLKDDLVLGDPDAPRFVLWNGKLRPVPAKPNDLPFFDLM 180

Query: 181 SIGGKIRAGFGALGIRPPPPGREESVEEFVRRNLGNEVFERLIEPFCSGVYAGDPSKLSM 240
           SIGGKIRAGFGALGIRPPPPGREESVEEFVRRNLGNEVFERLIEPFCSGVYAGDPSKLSM
Sbjct: 181 SIGGKIRAGFGALGIRPPPPGREESVEEFVRRNLGNEVFERLIEPFCSGVYAGDPSKLSM 240

Query: 241 KAAFGKVWRLEQNGGSIIGGTFKALQERNKTTKPPRDPRLPKPKGQTVGSFRKGLTMLPN 300
           KAAFGKVWRLEQNGGSIIGGTFKALQERNKTTKPPRDPRLPKPKGQTVGSFRKGLTMLPN
Sbjct: 241 KAAFGKVWRLEQNGGSIIGGTFKALQERNKTTKPPRDPRLPKPKGQTVGSFRKGLTMLPN 300

Query: 301 AISTCLGSKVKVSWKLSSISKVDDGGYSLTYETPEGLVSILSRSVIMTVPSYIAGTLLRP 360
           AISTCLGSKVKVSWKLSSISKVDDGGYSLTYETPEGLVSILSRSVIMTVPSYIAGTLLRP
Sbjct: 301 AISTCLGSKVKVSWKLSSISKVDDGGYSLTYETPEGLVSILSRSVIMTVPSYIAGTLLRP 360

Query: 361 ISGKAADALSKFYYPPVASVTISYPKGAIRKECLIDGELKGFGQLHPRSQGVTTLGTIYS 420
           ISGKAADALSKFYYPPVASVTISYPKGAIRKECLIDGELKGFGQLHPRSQGVTTLGTIYS
Sbjct: 361 ISGKAADALSKFYYPPVASVTISYPKGAIRKECLIDGELKGFGQLHPRSQGVTTLGTIYS 420

Query: 421 SSLFPNRAPDGRVLLLNYIGGATNTGILSQTESELIEVVDRDLRKILINPNAEDPLPLSV 480
           SSLFPNRAPDGRVLLLNYIGGATNTGILSQTESELIEVVDRDLRKILINPNAEDPLPLSV
Sbjct: 421 SSLFPNRAPDGRVLLLNYIGGATNTGILSQTESELIEVVDRDLRKILINPNAEDPLPLSV 480

Query: 481 RVWPQAIPQFLIGHLDVLDTAKAGLREAGMEGLFLGGNYVCGVALGRCVEGAYEAAAEVA 540
           RVWPQAIPQFLIGHLDVLDTAKAGLREAGMEGLFLGGNYVCGVALGRCVEGAYEAAAEVA
Sbjct: 481 RVWPQAIPQFLIGHLDVLDTAKAGLREAGMEGLFLGGNYVCGVALGRCVEGAYEAAAEVA 540

Query: 541 GFLSKKVYK 550
           GFLSKKVYK
Sbjct: 541 GFLSKKVYK 549

BLAST of CSPI05G02460 vs. NCBI nr
Match: gi|659072571|ref|XP_008466230.1| (PREDICTED: protoporphyrinogen oxidase 1, chloroplastic [Cucumis melo])

HSP 1 Score: 1086.6 bits (2809), Expect = 0.0e+00
Identity = 539/549 (98.18%), Postives = 546/549 (99.45%), Query Frame = 1

Query: 1   MATGSTLLTDLPFRRPHPLTLLRPSNIPSFHPLHISLQNNRLRSYFRCSIAEGSTALSPS 60
           MATG+TLLTDLPFRRPHPLTLLRPS+IPSF+PLHISLQNNRLRS+FRCSIAEGSTALSPS
Sbjct: 1   MATGATLLTDLPFRRPHPLTLLRPSDIPSFYPLHISLQNNRLRSHFRCSIAEGSTALSPS 60

Query: 61  NASSQSSILDCVVVGAGISGLCIAQALATKHPDVAPNIIVTEAKDRVGGNITTVERDGYL 120
           NASSQSSILDCVVVG GISGLCIAQALATKHPDVAPNIIVTEAKDRVGGNITTVERDGYL
Sbjct: 61  NASSQSSILDCVVVGGGISGLCIAQALATKHPDVAPNIIVTEAKDRVGGNITTVERDGYL 120

Query: 121 WEEGPNSFQPSDPILTMVVDSGLKDDLVLGDPDAPRFVLWNGKLRPVPAKPNDLPFFDLM 180
           WEEGPNSFQPSDPILTMVVDSGLKDDLVLGDPDAPRFVLWNGKLRPVPAKPNDLPFFDLM
Sbjct: 121 WEEGPNSFQPSDPILTMVVDSGLKDDLVLGDPDAPRFVLWNGKLRPVPAKPNDLPFFDLM 180

Query: 181 SIGGKIRAGFGALGIRPPPPGREESVEEFVRRNLGNEVFERLIEPFCSGVYAGDPSKLSM 240
           SIGGKIRAGFGALGIRPPPPGREESVEEFVRRNLG+EVFERLIEPFCSGVYAGDPSKLSM
Sbjct: 181 SIGGKIRAGFGALGIRPPPPGREESVEEFVRRNLGDEVFERLIEPFCSGVYAGDPSKLSM 240

Query: 241 KAAFGKVWRLEQNGGSIIGGTFKALQERNKTTKPPRDPRLPKPKGQTVGSFRKGLTMLPN 300
           KAAFGKVWRLEQNGGSIIGGTFKALQERNKTTKPPRDPRLPKPKGQTVGSFRKGLTMLPN
Sbjct: 241 KAAFGKVWRLEQNGGSIIGGTFKALQERNKTTKPPRDPRLPKPKGQTVGSFRKGLTMLPN 300

Query: 301 AISTCLGSKVKVSWKLSSISKVDDGGYSLTYETPEGLVSILSRSVIMTVPSYIAGTLLRP 360
           AISTCLGSKVK+SWKLSSISKVDDGGYSLTYETPEGLV ILSRSVIMTVPSYIAGTLLRP
Sbjct: 301 AISTCLGSKVKLSWKLSSISKVDDGGYSLTYETPEGLVPILSRSVIMTVPSYIAGTLLRP 360

Query: 361 ISGKAADALSKFYYPPVASVTISYPKGAIRKECLIDGELKGFGQLHPRSQGVTTLGTIYS 420
           ISGKAADALSKFYYPPVASVTISYPKGAIRKECLIDGELKGFGQLHPRSQGVTTLGTIYS
Sbjct: 361 ISGKAADALSKFYYPPVASVTISYPKGAIRKECLIDGELKGFGQLHPRSQGVTTLGTIYS 420

Query: 421 SSLFPNRAPDGRVLLLNYIGGATNTGILSQTESELIEVVDRDLRKILINPNAEDPLPLSV 480
           SSLFPNRAPDGRVLLLNYIGGATNTGILSQ ESELIEVVDRDLRKILINPNAEDPLPLSV
Sbjct: 421 SSLFPNRAPDGRVLLLNYIGGATNTGILSQKESELIEVVDRDLRKILINPNAEDPLPLSV 480

Query: 481 RVWPQAIPQFLIGHLDVLDTAKAGLREAGMEGLFLGGNYVCGVALGRCVEGAYEAAAEVA 540
           RVWPQAIPQFLIGHLD+LDTAKAGLREAGMEGLFLGGNYVCGVALGRCVEGAYEAAAEVA
Sbjct: 481 RVWPQAIPQFLIGHLDLLDTAKAGLREAGMEGLFLGGNYVCGVALGRCVEGAYEAAAEVA 540

Query: 541 GFLSKKVYK 550
           GFLSKKVYK
Sbjct: 541 GFLSKKVYK 549

BLAST of CSPI05G02460 vs. NCBI nr
Match: gi|502117534|ref|XP_004495854.1| (PREDICTED: protoporphyrinogen oxidase 1, chloroplastic [Cicer arietinum])

HSP 1 Score: 869.4 bits (2245), Expect = 3.4e-249
Identity = 443/558 (79.39%), Postives = 481/558 (86.20%), Query Frame = 1

Query: 6   TLLTDLPFRRPHPLTLLRPSNIPSFH---PLHIS--LQNNR---LRSYFRCSIAEGSTAL 65
           TL  DLPF  P   TLLRPS    FH   P+ IS  L+N+    LR   RCSIAE STA 
Sbjct: 5   TLFNDLPF--PPTQTLLRPSLHSPFHFSSPIPISKFLRNHHNPILR--LRCSIAEESTAS 64

Query: 66  SPSNASSQSSI------LDCVVVGAGISGLCIAQALATKHPDVAPNIIVTEAKDRVGGNI 125
               AS+  S+      +DCVVVG GISGLCI QAL+TKH +VAPN+IVTEA+DRVGGNI
Sbjct: 65  PTKTASNSGSVARGHSPVDCVVVGGGISGLCIVQALSTKHANVAPNVIVTEARDRVGGNI 124

Query: 126 TTVERDGYLWEEGPNSFQPSDPILTMVVDSGLKDDLVLGDPDAPRFVLWNGKLRPVPAKP 185
            TVERDGYLWEEGPNSFQPSDP+LTMVVDSGLKD+LVLGDPDAPRFVLWNGKLRPVP KP
Sbjct: 125 ITVERDGYLWEEGPNSFQPSDPMLTMVVDSGLKDELVLGDPDAPRFVLWNGKLRPVPGKP 184

Query: 186 NDLPFFDLMSIGGKIRAGFGALGIRPPPPGREESVEEFVRRNLGNEVFERLIEPFCSGVY 245
            DLPFFDLMSIGGK+RAGFGALGIRPPPPG EESVEEFVRRNLG+EVFERLIEPFCSGVY
Sbjct: 185 ADLPFFDLMSIGGKLRAGFGALGIRPPPPGYEESVEEFVRRNLGDEVFERLIEPFCSGVY 244

Query: 246 AGDPSKLSMKAAFGKVWRLEQNGGSIIGGTFKALQERNKTTKPPRDPRLPKPKGQTVGSF 305
           AGDPSKLSMKAAFGKVWRLEQNGGSIIGG+FKALQE+N  TKPPRDPRLPKPKGQTVGSF
Sbjct: 245 AGDPSKLSMKAAFGKVWRLEQNGGSIIGGSFKALQEKNGATKPPRDPRLPKPKGQTVGSF 304

Query: 306 RKGLTMLPNAISTCLGSKVKVSWKLSSISKVDDGGYSLTYETPEGLVSILSRSVIMTVPS 365
           RKGLTMLP AIS  LGSKVK+SWKL S+SK+D G YSLTYETPEG+VS+ S++V+MT+PS
Sbjct: 305 RKGLTMLPEAISARLGSKVKLSWKLLSVSKLDSGEYSLTYETPEGVVSLQSKTVVMTIPS 364

Query: 366 YIAGTLLRPISGKAADALSKFYYPPVASVTISYPKGAIRKECLIDGELKGFGQLHPRSQG 425
           ++A TLLRP++  AADALSKFYYPPVA+V+ISYPK AIR ECLIDGELKGFGQLHPRSQG
Sbjct: 365 HVASTLLRPLAPTAADALSKFYYPPVAAVSISYPKEAIRSECLIDGELKGFGQLHPRSQG 424

Query: 426 VTTLGTIYSSSLFPNRAPDGRVLLLNYIGGATNTGILSQTESELIEVVDRDLRKILINPN 485
           V TLGTIYSSSLFPNRAP GRVLLLNYIGGATN GILS+TESEL+E VDRDLR ILINPN
Sbjct: 425 VQTLGTIYSSSLFPNRAPPGRVLLLNYIGGATNPGILSKTESELVEAVDRDLRNILINPN 484

Query: 486 AEDPLPLSVRVWPQAIPQFLIGHLDVLDTAKAGLREAGMEGLFLGGNYVCGVALGRCVEG 545
           A DPL L VR+WPQAIPQFLIGHLD+LD AK  L+  G EGLFLGGNYV GVALGRCVEG
Sbjct: 485 APDPLFLGVRLWPQAIPQFLIGHLDLLDAAKVSLKSTGFEGLFLGGNYVTGVALGRCVEG 544

Query: 546 AYEAAAEVAGFLSKKVYK 550
           AYE AAEV  FLS++VYK
Sbjct: 545 AYEVAAEVNDFLSQRVYK 558

BLAST of CSPI05G02460 vs. NCBI nr
Match: gi|922404024|ref|XP_013469143.1| (protoporphyrinogen IX oxidase [Medicago truncatula])

HSP 1 Score: 860.9 bits (2223), Expect = 1.2e-246
Identity = 434/545 (79.63%), Postives = 472/545 (86.61%), Query Frame = 1

Query: 16  PHPLTLLRP---SNIPSFHPLHISLQNNRLRS---YFRCSIAEGSTALSPSNASS----- 75
           P   TLLRP   S  PS +     ++N    +     RCSIAE STA SP+  +S     
Sbjct: 8   PPTQTLLRPYLHSPFPSPNSKSKFIRNRNHHNPILRLRCSIAEESTA-SPTKTTSKSNSG 67

Query: 76  QSSILDCVVVGAGISGLCIAQALATKHPDVAPNIIVTEAKDRVGGNITTVERDGYLWEEG 135
           +SS +DCVVVG GISGLCIAQAL+TKH    PN++VTEAKDRVGGNI TVERDG+LWEEG
Sbjct: 68  ESSSVDCVVVGGGISGLCIAQALSTKHAHGVPNVVVTEAKDRVGGNIITVERDGFLWEEG 127

Query: 136 PNSFQPSDPILTMVVDSGLKDDLVLGDPDAPRFVLWNGKLRPVPAKPNDLPFFDLMSIGG 195
           PNSFQPSDP+LTMVVDSGLKDDLVLGDPDAPRFVLWNGKLRPVP KP DLPFFDLMSIGG
Sbjct: 128 PNSFQPSDPMLTMVVDSGLKDDLVLGDPDAPRFVLWNGKLRPVPGKPTDLPFFDLMSIGG 187

Query: 196 KIRAGFGALGIRPPPPGREESVEEFVRRNLGNEVFERLIEPFCSGVYAGDPSKLSMKAAF 255
           KIRAGFGALGIRPPPPG EESVEEFVRRNLG EVFERLIEPFCSGVYAGDPSKLSMKAAF
Sbjct: 188 KIRAGFGALGIRPPPPGHEESVEEFVRRNLGAEVFERLIEPFCSGVYAGDPSKLSMKAAF 247

Query: 256 GKVWRLEQNGGSIIGGTFKALQERNKTTKPPRDPRLPKPKGQTVGSFRKGLTMLPNAIST 315
           GKVWRLEQNGGSIIGG FKA+QERNK +KPPRDPRLPKPKGQTVGSFRKGLTMLPNAIS 
Sbjct: 248 GKVWRLEQNGGSIIGGAFKAIQERNKVSKPPRDPRLPKPKGQTVGSFRKGLTMLPNAISA 307

Query: 316 CLGSKVKVSWKLSSISKVDDGGYSLTYETPEGLVSILSRSVIMTVPSYIAGTLLRPISGK 375
            LG+KVK+SWKL SISK+D G YSLTYETPEG+VS+ S++VIMT+PS++A  LLRP+S  
Sbjct: 308 RLGNKVKLSWKLLSISKLDSGEYSLTYETPEGVVSLQSKTVIMTIPSHVASPLLRPLSPA 367

Query: 376 AADALSKFYYPPVASVTISYPKGAIRKECLIDGELKGFGQLHPRSQGVTTLGTIYSSSLF 435
           AADALSKFYYPPVA+V+ISYPK AIR ECLIDGELKGFGQLHPRSQGV TLGTIYSSSLF
Sbjct: 368 AADALSKFYYPPVAAVSISYPKEAIRSECLIDGELKGFGQLHPRSQGVQTLGTIYSSSLF 427

Query: 436 PNRAPDGRVLLLNYIGGATNTGILSQTESELIEVVDRDLRKILINPNAEDPLPLSVRVWP 495
           PNRAP GRVLLLNYIGGATN+GILS+TE EL+E VDRDLRKILINPNA+DP+ L VRVWP
Sbjct: 428 PNRAPPGRVLLLNYIGGATNSGILSKTEGELVEAVDRDLRKILINPNAQDPIVLGVRVWP 487

Query: 496 QAIPQFLIGHLDVLDTAKAGLREAGMEGLFLGGNYVCGVALGRCVEGAYEAAAEVAGFLS 550
           QAIPQFL+GHLD+LD AK  L+  G EGLFLGGNYV GVALGRCVEGAYE AAEV  FLS
Sbjct: 488 QAIPQFLVGHLDLLDAAKVSLKNTGFEGLFLGGNYVSGVALGRCVEGAYEIAAEVNDFLS 547

BLAST of CSPI05G02460 vs. NCBI nr
Match: gi|823187671|ref|XP_012490260.1| (PREDICTED: protoporphyrinogen oxidase 1, chloroplastic [Gossypium raimondii])

HSP 1 Score: 852.4 bits (2201), Expect = 4.3e-244
Identity = 422/535 (78.88%), Postives = 465/535 (86.92%), Query Frame = 1

Query: 19  LTLLRPSNIPSFHPLHISLQNN--RLRSYF--RCSIAEGSTALSPSNASSQSSILDCVVV 78
           L+LLR S  PS  P  I    +  R R  F  RCS+AEG T  S      +SSI DCV+V
Sbjct: 7   LSLLRSS--PSVSPFSIPHHQHPPRFRKPFKLRCSLAEGPTISSSKIDGGESSIADCVIV 66

Query: 79  GAGISGLCIAQALATKHPDVAPNIIVTEAKDRVGGNITTVERDGYLWEEGPNSFQPSDPI 138
           G GISGLCIAQALATKH DVA N+IVTEA+DRVGGNITTVERDGYLWEEGPNSFQPSDPI
Sbjct: 67  GGGISGLCIAQALATKHRDVASNVIVTEARDRVGGNITTVERDGYLWEEGPNSFQPSDPI 126

Query: 139 LTMVVDSGLKDDLVLGDPDAPRFVLWNGKLRPVPAKPNDLPFFDLMSIGGKIRAGFGALG 198
           LTM VDSGLKDDLVLGDP+APRFVLW GKLRPVP+KP DLPFFDLMSI GK+RAGFGA+G
Sbjct: 127 LTMAVDSGLKDDLVLGDPNAPRFVLWEGKLRPVPSKPTDLPFFDLMSIAGKLRAGFGAIG 186

Query: 199 IRPPPPGREESVEEFVRRNLGNEVFERLIEPFCSGVYAGDPSKLSMKAAFGKVWRLEQNG 258
           IRPPPPG EESVEEFVRRNLG EVFER IEPFCSGVYAGDPSKLSMKAAFG+VW+LE+ G
Sbjct: 187 IRPPPPGYEESVEEFVRRNLGAEVFERFIEPFCSGVYAGDPSKLSMKAAFGRVWKLEEIG 246

Query: 259 GSIIGGTFKALQERNKTTKPPRDPRLPKPKGQTVGSFRKGLTMLPNAISTCLGSKVKVSW 318
           GSIIGGTFK +QERNKT KPPRDPRLPKPKGQTVGSFRKGLTMLP AI+  LGS VK+SW
Sbjct: 247 GSIIGGTFKTIQERNKTPKPPRDPRLPKPKGQTVGSFRKGLTMLPEAIANSLGSNVKLSW 306

Query: 319 KLSSISKVDDGGYSLTYETPEGLVSILSRSVIMTVPSYIAGTLLRPISGKAADALSKFYY 378
           KLSSI+K+ +GGY+L +ETPEG+VS+ SRSV+MT+PS++A  LL P+S  AADALS+FYY
Sbjct: 307 KLSSITKLGNGGYNLQFETPEGMVSLQSRSVVMTIPSHVASNLLHPLSAAAADALSQFYY 366

Query: 379 PPVASVTISYPKGAIRKECLIDGELKGFGQLHPRSQGVTTLGTIYSSSLFPNRAPDGRVL 438
           PPVASVT+SYPK AIRKECLIDGELKGFGQLHPRSQG+ TLGTIYSSSLFPNRAP GRVL
Sbjct: 367 PPVASVTVSYPKEAIRKECLIDGELKGFGQLHPRSQGIETLGTIYSSSLFPNRAPSGRVL 426

Query: 439 LLNYIGGATNTGILSQTESELIEVVDRDLRKILINPNAEDPLPLSVRVWPQAIPQFLIGH 498
           LLNYIGGATNTGILS+TE EL+E VDRDLRK+LINPNA+DPL L VRVWP+AIPQFL+GH
Sbjct: 427 LLNYIGGATNTGILSKTEGELVEAVDRDLRKMLINPNAKDPLVLGVRVWPKAIPQFLVGH 486

Query: 499 LDVLDTAKAGLREAGMEGLFLGGNYVCGVALGRCVEGAYEAAAEVAGFLSKKVYK 550
           LD+LD+AK  LR++G  GLFLGGNYV GVALGRCVEGAYE AAEV  FLS+  YK
Sbjct: 487 LDLLDSAKMALRDSGFHGLFLGGNYVSGVALGRCVEGAYEVAAEVKEFLSQYAYK 539

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
PPOC_ARATH1.0e-23776.30Protoporphyrinogen oxidase 1, chloroplastic OS=Arabidopsis thaliana GN=PPOX1 PE=... [more]
PPOC_TOBAC1.1e-22675.94Protoporphyrinogen oxidase, chloroplastic OS=Nicotiana tabacum GN=PPXI PE=2 SV=1[more]
PPOC_ORYSJ3.1e-22674.33Protoporphyrinogen oxidase, chloroplastic OS=Oryza sativa subsp. japonica GN=PPO... [more]
PPOX_MYXXA2.2e-5432.14Protoporphyrinogen oxidase OS=Myxococcus xanthus GN=hemY PE=1 SV=1[more]
PPOCM_SPIOL2.3e-4829.65Protoporphyrinogen oxidase, chloroplastic/mitochondrial OS=Spinacia oleracea GN=... [more]
Match NameE-valueIdentityDescription
A0A0A0KMZ2_CUCSA0.0e+00100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_5G128250 PE=4 SV=1[more]
A0A072VNG3_MEDTR8.4e-24779.63Protoporphyrinogen IX oxidase OS=Medicago truncatula GN=MTR_1g085730 PE=4 SV=1[more]
A0A0D2SID4_GOSRA3.0e-24478.88Uncharacterized protein OS=Gossypium raimondii GN=B456_007G117800 PE=4 SV=1[more]
A0A151SSA5_CAJCA3.9e-24477.09Uncharacterized protein OS=Cajanus cajan GN=KK1_003887 PE=4 SV=1[more]
A0A061DRW0_THECC6.6e-24477.94Flavin containing amine oxidoreductase family OS=Theobroma cacao GN=TCM_004957 P... [more]
Match NameE-valueIdentityDescription
AT4G01690.15.7e-23976.30 Flavin containing amine oxidoreductase family[more]
AT5G14220.13.7e-4428.48 Flavin containing amine oxidoreductase family[more]
Match NameE-valueIdentityDescription
gi|449463515|ref|XP_004149479.1|0.0e+00100.00PREDICTED: protoporphyrinogen oxidase 1, chloroplastic [Cucumis sativus][more]
gi|659072571|ref|XP_008466230.1|0.0e+0098.18PREDICTED: protoporphyrinogen oxidase 1, chloroplastic [Cucumis melo][more]
gi|502117534|ref|XP_004495854.1|3.4e-24979.39PREDICTED: protoporphyrinogen oxidase 1, chloroplastic [Cicer arietinum][more]
gi|922404024|ref|XP_013469143.1|1.2e-24679.63protoporphyrinogen IX oxidase [Medicago truncatula][more]
gi|823187671|ref|XP_012490260.1|4.3e-24478.88PREDICTED: protoporphyrinogen oxidase 1, chloroplastic [Gossypium raimondii][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR002937Amino_oxidase
IPR004572Protoporphyrinogen_oxidase
IPR023753FAD/NAD-binding_dom
IPR027418Protoporphyrinogen oxidase, C-terminal domain
Vocabulary: Molecular Function
TermDefinition
GO:0016491oxidoreductase activity
GO:0004729oxygen-dependent protoporphyrinogen oxidase activity
Vocabulary: Biological Process
TermDefinition
GO:0055114oxidation-reduction process
GO:0006779porphyrin-containing compound biosynthetic process
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015994 chlorophyll metabolic process
biological_process GO:0055114 oxidation-reduction process
biological_process GO:0006779 porphyrin-containing compound biosynthetic process
biological_process GO:0006782 protoporphyrinogen IX biosynthetic process
cellular_component GO:0009941 chloroplast envelope
cellular_component GO:0009534 chloroplast thylakoid
cellular_component GO:0005575 cellular_component
molecular_function GO:0004729 oxygen-dependent protoporphyrinogen oxidase activity
molecular_function GO:0016491 oxidoreductase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI05G02460.1CSPI05G02460.1mRNA


Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002937Amine oxidasePFAMPF01593Amino_oxidasecoord: 78..540
score: 1.1
IPR004572Protoporphyrinogen oxidaseTIGRFAMsTIGR00562TIGR00562coord: 70..544
score: 7.6E
IPR023753FAD/NAD(P)-binding domainGENE3DG3DSA:3.50.50.60coord: 63..122
score: 1.4
IPR023753FAD/NAD(P)-binding domainunknownSSF51905FAD/NAD(P)-binding domaincoord: 70..389
score: 6.15E-46coord: 508..540
score: 6.15
IPR027418Protoporphyrinogen oxidase, C-terminal domainGENE3DG3DSA:3.90.660.20coord: 305..489
score: 3.6
NoneNo IPR availableGENE3DG3DSA:1.10.3110.10coord: 167..266
score: 1.2
NoneNo IPR availablePANTHERPTHR10742AMINE OXIDASEcoord: 36..547
score: 9.5E
NoneNo IPR availablePANTHERPTHR10742:SF294PROTOPORPHYRINOGEN OXIDASE 1, CHLOROPLASTICcoord: 36..547
score: 9.5E
NoneNo IPR availableunknownSSF54373FAD-linked reductases, C-terminal domaincoord: 373..489
score: 6.73