CSPI05G02400 (gene) Wild cucumber (PI 183967)

BLAST of CSPI05G02400 vs. Swiss-Prot
Match: PME28_ARATH (Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana GN=PME28 PE=2 SV=1)

HSP 1 Score: 444.9 bits (1143), Expect = 1.3e-123
Identity = 221/562 (39.32%), Postives = 348/562 (61.92%), Query Frame = 1

Query: 4   KVIVSGLSLILVVGIALAIAATINKSNASNGGTEDVSPKMKAVSAICSTTDYQDECKTTL 63
           + ++  +S +L++ + +A+   ++ + + N G E+++  +KA+  +C+ TDY++ C+ TL
Sbjct: 15  RYVIISISSVLLISMVVAVTIGVSVNKSDNAGDEEITTSVKAIKDVCAPTDYKETCEDTL 74

Query: 64  DHVARNTSSNDPKDYAEAAILATIGEITKGYNLSDSLIVEASTNASIKMSVEDCKDLLQF 123
              A++TS  DP +  + A  AT+ +I+     S ++I E   +   KM+++ CK+L+ +
Sbjct: 75  RKDAKDTS--DPLELVKTAFNATMKQISDVAKKSQTMI-ELQKDPRAKMALDQCKELMDY 134

Query: 124 AIDELQASYSAVGESDLHTDADRVADIKNWLSAVISYQQSCLDGLGEFDPQLKQRMQDGL 183
           AI EL  S+  +G+ + H   + +  ++ WLSA IS++Q+CLDG         + ++  L
Sbjct: 135 AIGELSKSFEELGKFEFHKVDEALVKLRIWLSATISHEQTCLDGFQGTQGNAGETIKKAL 194

Query: 184 DVAGKLTSNALAIVTAVSNILDNYRLQLKVQPSGRRLLGTTVVDRDGFPTWLTGADRKLL 243
             A +LT N LA+VT +SN L   ++    + + RRLL         FP+W+    R+LL
Sbjct: 195 KTAVQLTHNGLAMVTEMSNYLGQMQIP---EMNSRRLLS------QEFPSWMDARARRLL 254

Query: 244 ASKQGGVRPTPNAVVAKDGSGKYKTIAAALAAYPKVLRGRYVIYVKAGIYDEYITLTKDM 303
            +    V+  P+ VVA+DGSG+YKTI  AL   PK     +V+++K GIY EY+ + + M
Sbjct: 255 NAPMSEVK--PDIVVAQDGSGQYKTINEALNFVPKKKNTTFVVHIKEGIYKEYVQVNRSM 314

Query: 304 KNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQAVA 363
            ++   GDGP KT+++G KS +DG TT  TA+ A +G+ F+ K++ F NTAG   HQAVA
Sbjct: 315 THLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAFENTAGAIKHQAVA 374

Query: 364 LRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVVSGTVDFIFGDSSTVIQNSLIIVRR 423
           +RV +D S F+NC+ DG+QDTLY  +HRQFYR+C +SGT+DF+FGD++ V QN  ++VR+
Sbjct: 375 IRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAAAVFQNCTLLVRK 434

Query: 424 PMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYARTVIM 483
           P+ NQ   +TA GR + +E TG V+  C IV E    A + +  T+LGRPWK+Y+RT+IM
Sbjct: 435 PLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYLGRPWKEYSRTIIM 494

Query: 484 ESTLGDFIQPAGYMPWSGDFALETCLYLEYGNRGPGANTNRRVRWKGAKVIGRNEALQYT 543
            + + DF+ P G+ PW G+F L T  Y E  N GPGA   +RV W G K +   E L++T
Sbjct: 495 NTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPGIKKLSDEEILKFT 554

Query: 544 AGAFLLGRSWLPTTGGLYYLGL 566
              ++ G +W+P  G  Y LGL
Sbjct: 555 PAQYIQGDAWIPGKGVPYILGL 562

BLAST of CSPI05G02400 vs. Swiss-Prot
Match: PME21_ARATH (Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana GN=PME21 PE=2 SV=2)

HSP 1 Score: 442.6 bits (1137), Expect = 6.6e-123
Identity = 223/561 (39.75%), Postives = 343/561 (61.14%), Query Frame = 1

Query: 5   VIVSGLSLILVVGIALAIAATINKSNASNGGTEDVSPKMKAVSAICSTTDYQDECKTTLD 64
           + +S + LI +V +A+ +  ++NK +  + G  +V+  +KAV  +C+ TDY+  C+ TL 
Sbjct: 18  ITISSVLLISMV-VAVTVGVSLNKHDGDSKGKAEVNASVKAVKDVCAPTDYRKTCEDTLI 77

Query: 65  HVARNTSSNDPKDYAEAAILATIGEITKGYNLSDSLIVEASTNASIKMSVEDCKDLLQFA 124
              +NT+  DP +  + A   T+ +IT     S + I+E   ++  +M+++ CK+L+ +A
Sbjct: 78  KNGKNTT--DPMELVKTAFNVTMKQITDAAKKSQT-IMELQKDSRTRMALDQCKELMDYA 137

Query: 125 IDELQASYSAVGESDLHTDADRVADIKNWLSAVISYQQSCLDGLGEFDPQLKQRMQDGLD 184
           +DEL  S+  +G+ + H   + + +++ WLSA IS++++CL+G         + M+  L 
Sbjct: 138 LDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCLEGFQGTQGNAGETMKKALK 197

Query: 185 VAGKLTSNALAIVTAVSNILDNYRLQLKVQPSGRRLLGTTVVDRDGFPTWLTGADRKLLA 244
            A +LT N LAI++ +SN +   ++      + RRLL       +GFP+W+    RKLL 
Sbjct: 198 TAIELTHNGLAIISEMSNFVGQMQIP---GLNSRRLLA------EGFPSWVDQRGRKLLQ 257

Query: 245 SKQGGVRPTPNAVVAKDGSGKYKTIAAALAAYPKVLRGRYVIYVKAGIYDEYITLTKDMK 304
           +        P+ VVA+DGSG+YKTI  AL   PK     +V+++KAG+Y EY+ + K M 
Sbjct: 258 AAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIKAGLYKEYVQVNKTMS 317

Query: 305 NVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQAVAL 364
           ++   GDGP KTI++G K+ +DG TT  TA+ A +G  F+ K++GF NTAG   HQAVA+
Sbjct: 318 HLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIGFENTAGAIKHQAVAV 377

Query: 365 RVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVVSGTVDFIFGDSSTVIQNSLIIVRRP 424
           RVQSD S FFNCR DG+QDTLY  +HRQF+R+C +SGT+DF+FGD++ V QN  ++VR+P
Sbjct: 378 RVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDAAAVFQNCTLLVRKP 437

Query: 425 MDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYARTVIME 484
           + NQ   +TA GR + +E TG V   C I  E    A +     +LGRPWK+Y+RT+IM 
Sbjct: 438 LPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAYLGRPWKEYSRTIIMN 497

Query: 485 STLGDFIQPAGYMPWSGDFALETCLYLEYGNRGPGANTNRRVRWKGAKVIGRNEALQYTA 544
           + + DF+QP G+ PW GDF L+T  Y E  N GPG+    RV W G K +   + L++T 
Sbjct: 498 TFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWAGIKTLSEEDILKFTP 557

Query: 545 GAFLLGRSWLPTTGGLYYLGL 566
             ++ G  W+P  G  Y  GL
Sbjct: 558 AQYIQGDDWIPGKGVPYTTGL 565

BLAST of CSPI05G02400 vs. Swiss-Prot
Match: PME4_ARATH (Pectinesterase 4 OS=Arabidopsis thaliana GN=PME4 PE=2 SV=1)

HSP 1 Score: 427.9 bits (1099), Expect = 1.7e-118
Identity = 255/604 (42.22%), Postives = 358/604 (59.27%), Query Frame = 1

Query: 1   MLGKVIVSGLSLILVVGIALAIAATINKSNASNGGTEDVSPKMKAVSAICSTTDYQDECK 60
           M+GKV+VS  S++L+VG+A+ + A INK+     G  ++SP+MKAV  IC +T  +  C 
Sbjct: 1   MIGKVVVSVASILLIVGVAIGVVAFINKN-----GDANLSPQMKAVQGICQSTSDKASCV 60

Query: 61  TTLDHVARNTSSNDPKDYAEAAILATIGEITKGYNLSDSLIVE--ASTNASIKMSVEDCK 120
            TL+ V     S DP    +A +LAT  E+TK  N +    V   +S + + K  ++ CK
Sbjct: 61  KTLEPV----KSEDPNKLIKAFMLATKDELTKSSNFTGQTEVNMGSSISPNNKAVLDYCK 120

Query: 121 DLLQFAIDELQASYSAVGESDLHTDADRVADIKNWLSAVISYQQSCLDGLGEFDPQLKQR 180
            +  +A+++L      +GE DL     ++  +K WL  V +YQ  CLD + E D  L++ 
Sbjct: 121 RVFMYALEDLATIIEEMGE-DLSQIGSKIDQLKQWLIGVYNYQTDCLDDIEEDD--LRKA 180

Query: 181 MQDGLDVAGKLTSNALAI-------VTAVSNILDNYRLQLKVQPS--------------- 240
           + +G+  +  LT+NA+ I       +  ++N +D+ +      P+               
Sbjct: 181 IGEGIANSKILTTNAIDIFHTVVSAMAKINNKVDDLKNMTGGIPTPGAPPVVDESPVADP 240

Query: 241 ---GRRLLGTTVVDRDGFPTWLTGADRKLLA--------SKQGGVRPTPNAVVAKDGSGK 300
               RRLL    +D  G PTW++GADRKL+A         + GG R   N VVAKDGSG+
Sbjct: 241 DGPARRLLED--IDETGIPTWVSGADRKLMAKAGRGRRGGRGGGARVRTNFVVAKDGSGQ 300

Query: 301 YKTIAAALAAYPKVLRGRYVIYVKAGIYDEYITLTKDMKNVFMYGDGPRKTIVTGRKS-- 360
           +KT+  A+ A P+  RGR +IY+KAG+Y E + + K   N+FM+GDG RKT+++  +S  
Sbjct: 301 FKTVQQAVDACPENNRGRCIIYIKAGLYREQVIIPKKKNNIFMFGDGARKTVISYNRSVA 360

Query: 361 NRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQAVALRVQSDRSAFFNCRMDGHQD 420
              G TT  +A+     EGF+ K MGF NTAGP GHQA A+RV  DR+  FNCR DG+QD
Sbjct: 361 LSRGTTTSLSATVQVESEGFMAKWMGFKNTAGPMGHQAAAIRVNGDRAVIFNCRFDGYQD 420

Query: 421 TLYVQTHRQFYRNCVVSGTVDFIFGDSSTVIQNSLIIVRRPMDNQQNTVTAQGRAEQKEI 480
           TLYV   RQFYRNCVVSGTVDFIFG S+TVIQN+LI+VR+    Q NTVTA G      +
Sbjct: 421 TLYVNNGRQFYRNCVVSGTVDFIFGKSATVIQNTLIVVRKGSKGQYNTVTADGNELGLGM 480

Query: 481 -TGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYARTVIMESTLGDFIQPAGYMPWSGD 540
             G+V+ NCRIVP++KL  ER  + T+LGRPWK+++ TVIM + +GD I+P G+  W G+
Sbjct: 481 KIGIVLQNCRIVPDRKLTPERLTVATYLGRPWKKFSTTVIMSTEMGDLIRPEGWKIWDGE 540

Query: 541 FALETCLYLEYGNRGPGANTNRRVRWKGAKVI-GRNEALQYTAGAFLLGRSWL-----PT 561
              ++C Y+EY NRGPGA  NRRV W  AKV     E   +TA  +L   +W+     P 
Sbjct: 541 SFHKSCRYVEYNNRGPGAFANRRVNW--AKVARSAAEVNGFTAANWLGPINWIQEANVPV 588

BLAST of CSPI05G02400 vs. Swiss-Prot
Match: PME45_ARATH (Putative pectinesterase/pectinesterase inhibitor 45 OS=Arabidopsis thaliana GN=PME45 PE=2 SV=1)

HSP 1 Score: 427.9 bits (1099), Expect = 1.7e-118
Identity = 248/600 (41.33%), Postives = 354/600 (59.00%), Query Frame = 1

Query: 4   KVIVSGLSLILVVGIALAIAA----------------TINKSNASNGGTEDVSPKM---- 63
           K I+ G+  +LVV  A+   A                T NKS  S   +E  SPK     
Sbjct: 23  KRIILGVVSVLVVAAAIIGGAFAYVTYENKTQEQGKTTNNKSKDSPTKSESPSPKPPSSA 82

Query: 64  ----------KAVSAICSTTDYQDECKTTL-DHVARNTSSNDPKDYAEAAILATIGEITK 123
                     K +  +C++T Y+  C+ TL +   ++T   DP+   ++AI+A   ++ +
Sbjct: 83  AQTVKAGQVDKIIQTLCNSTLYKPTCQNTLKNETKKDTPQTDPRSLLKSAIVAVNDDLDQ 142

Query: 124 GYNLSDSLIVEASTNASIKMSVEDCKDLLQFAIDELQASYSAVGESDLHTDADRVADIKN 183
            +    SL  E   N   K ++  CK L+  A +EL  S   + +S+++  A  V D+ +
Sbjct: 143 VFKRVLSLKTE---NKDDKDAIAQCKLLVDEAKEELGTSMKRINDSEVNNFAKIVPDLDS 202

Query: 184 WLSAVISYQQSCLDGLGEFDPQLKQRMQDGLDVAGKLTSNALAIVTAVSNILDNY----- 243
           WLSAV+SYQ++C+DG  E   +LK  ++   + +  LTSN+LA++ ++   L +      
Sbjct: 203 WLSAVMSYQETCVDGFEE--GKLKTEIRKNFNSSQVLTSNSLAMIKSLDGYLSSVPKVKT 262

Query: 244 RLQLKVQPSGRRLLGTTVVDRDGFPTWLTGADRKLLASKQGGVRPT-PNAVVAKDGSGKY 303
           RL L+ + S +        + D   +WL+  +R++L  K   V+   PNA VAKDGSG +
Sbjct: 263 RLLLEARSSAK--------ETDHITSWLSNKERRML--KAVDVKALKPNATVAKDGSGNF 322

Query: 304 KTIAAALAAYPKVLRGRYVIYVKAGIYDEYITLTKDMKNVFMYGDGPRKTIVTGRKSNRD 363
            TI AAL A P   +GRY IY+K GIYDE + + K   NV M GDG +KTIVTG KS+  
Sbjct: 323 TTINAALKAMPAKYQGRYTIYIKHGIYDESVIIDKKKPNVTMVGDGSQKTIVTGNKSHAK 382

Query: 364 GFTTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQAVALRVQSDRSAFFNCRMDGHQDTLY 423
              T  TA+F A GEGF+ +SMGF NTAGPEGHQAVA+RVQSDRS F NCR +G+QDTLY
Sbjct: 383 KIRTFLTATFVAQGEGFMAQSMGFRNTAGPEGHQAVAIRVQSDRSVFLNCRFEGYQDTLY 442

Query: 424 VQTHRQFYRNCVVSGTVDFIFGDSSTVIQNSLIIVRRPMDNQQNTVTAQGRAEQKEITGL 483
             THRQ+YR+CV+ GTVDFIFGD++ + QN  I +R+ +  Q+NTVTAQGR ++ + TG 
Sbjct: 443 AYTHRQYYRSCVIIGTVDFIFGDAAAIFQNCDIFIRKGLPGQKNTVTAQGRVDKFQTTGF 502

Query: 484 VIHNCRIVPEQKLFAERFKIPTFLGRPWKQYARTVIMESTLGDFIQPAGYMPW-SGDFAL 543
           VIHNC + P + L   + +  ++LGRPWK ++RTV+MEST+ D I P G++ W   DFA+
Sbjct: 503 VIHNCTVAPNEDLKPVKAQFKSYLGRPWKPHSRTVVMESTIEDVIDPVGWLRWQETDFAI 562

Query: 544 ETCLYLEYGNRGPGANTNRRVRWKGAKVIGRNEALQYTAGAFLLGRSWLPTTGGLYYLGL 566
           +T  Y EY N GP   T  RV+W G +V+ + EA+++T G FL G  W+   G    LGL
Sbjct: 563 DTLSYAEYKNDGPSGATAARVKWPGFRVLNKEEAMKFTVGPFLQG-EWIQAIGSPVKLGL 606

BLAST of CSPI05G02400 vs. Swiss-Prot
Match: PME13_ARATH (Probable pectinesterase/pectinesterase inhibitor 13 OS=Arabidopsis thaliana GN=PME13 PE=2 SV=2)

HSP 1 Score: 425.2 bits (1092), Expect = 1.1e-117
Identity = 231/524 (44.08%), Postives = 330/524 (62.98%), Query Frame = 1

Query: 44  KAVSAICSTTDYQDECKTTL-DHVARNTSSNDPKDYAEAAILATIGEITKGYNLSDSLIV 103
           K +  +CS+T Y   C+ TL +   +  + ++P  + ++AI A   ++        SL  
Sbjct: 107 KIIQTLCSSTLYMQICEKTLKNRTDKGFALDNPTTFLKSAIEAVNEDLDLVLEKVLSLKT 166

Query: 104 EASTNASIKMSVEDCKDLLQFAIDELQASYSAVGESDLHTDADRVADIKNWLSAVISYQQ 163
           E   N   K ++E CK L++ A +E  AS + +  +++++    V D+++WLSAV+SYQ+
Sbjct: 167 E---NQDDKDAIEQCKLLVEDAKEETVASLNKINVTEVNSFEKVVPDLESWLSAVMSYQE 226

Query: 164 SCLDGLGEFDPQLKQRMQDGLDVAGKLTSNALAIVTAVSNILDNYRLQLKVQPSGRRLLG 223
           +CLDG  E +  LK  ++  ++ +  LTSN+LA++   +   +N    +KV    R LL 
Sbjct: 227 TCLDGFEEGN--LKSEVKTSVNSSQVLTSNSLALIKTFT---ENLSPVMKVVE--RHLL- 286

Query: 224 TTVVDRDGFPTWLTGADRKLLASKQGGVRPTPNAVVAKDGSGKYKTIAAALAAYPKVLRG 283
                 D  P+W++  DR++L +        PNA VAKDGSG + TI  AL A P+   G
Sbjct: 287 ------DDIPSWVSNDDRRMLRAVDVKALK-PNATVAKDGSGDFTTINDALRAMPEKYEG 346

Query: 284 RYVIYVKAGIYDEYITLTKDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEG 343
           RY+IYVK GIYDEY+T+ K   N+ M GDG +KTIVTG KS+     T  TA+F A GEG
Sbjct: 347 RYIIYVKQGIYDEYVTVDKKKANLTMVGDGSQKTIVTGNKSHAKKIRTFLTATFVAQGEG 406

Query: 344 FLCKSMGFTNTAGPEGHQAVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVVSGT 403
           F+ +SMGF NTAGPEGHQAVA+RVQSDRS F NCR +G+QDTLY  THRQ+YR+CV+ GT
Sbjct: 407 FMAQSMGFRNTAGPEGHQAVAIRVQSDRSIFLNCRFEGYQDTLYAYTHRQYYRSCVIVGT 466

Query: 404 VDFIFGDSSTVIQNSLIIVRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAE 463
           +DFIFGD++ + QN  I +R+ +  Q+NTVTAQGR ++ + TG V+HNC+I   + L   
Sbjct: 467 IDFIFGDAAAIFQNCNIFIRKGLPGQKNTVTAQGRVDKFQTTGFVVHNCKIAANEDLKPV 526

Query: 464 RFKIPTFLGRPWKQYARTVIMESTLGDFIQPAGYMPW-SGDFALETCLYLEYGNRGPGAN 523
           + +  ++LGRPWK Y+RT+IMES + + I P G++ W   DFA++T  Y EY N+G   +
Sbjct: 527 KEEYKSYLGRPWKNYSRTIIMESKIENVIDPVGWLRWQETDFAIDTLYYAEYNNKGSSGD 586

Query: 524 TNRRVRWKGAKVIGRNEALQYTAGAFLLGRSWLPTTGGLYYLGL 566
           T  RV+W G KVI + EAL YT G FL G  W+  +G    LGL
Sbjct: 587 TTSRVKWPGFKVINKEEALNYTVGPFLQG-DWISASGSPVKLGL 611

BLAST of CSPI05G02400 vs. TrEMBL
Match: A0A0A0KJ73_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_5G125700 PE=4 SV=1)

HSP 1 Score: 1122.8 bits (2903), Expect = 0.0e+00
Identity = 565/567 (99.65%), Postives = 566/567 (99.82%), Query Frame = 1

Query: 1   MLGKVIVSGLSLILVVGIALAIAATINKSNASNGGTEDVSPKMKAVSAICSTTDYQDECK 60
           MLGKVIVSGLSLILVVGIALAIAATINKSNASNGGTEDVSPKMKAVSAICSTTDYQDECK
Sbjct: 1   MLGKVIVSGLSLILVVGIALAIAATINKSNASNGGTEDVSPKMKAVSAICSTTDYQDECK 60

Query: 61  TTLDHVARNTSSNDPKDYAEAAILATIGEITKGYNLSDSLIVEASTNASIKMSVEDCKDL 120
           TTLDHVARNTSSNDPKDYAEAAILATIGEITKGYNLSDSLIVEASTNASIKMSVEDCKDL
Sbjct: 61  TTLDHVARNTSSNDPKDYAEAAILATIGEITKGYNLSDSLIVEASTNASIKMSVEDCKDL 120

Query: 121 LQFAIDELQASYSAVGESDLHTDADRVADIKNWLSAVISYQQSCLDGLGEFDPQLKQRMQ 180
           LQFAIDELQASYSAVGESDLHTD+DRVADIKNWLSAVISYQQSCLDGLGEFDPQLKQRMQ
Sbjct: 121 LQFAIDELQASYSAVGESDLHTDSDRVADIKNWLSAVISYQQSCLDGLGEFDPQLKQRMQ 180

Query: 181 DGLDVAGKLTSNALAIVTAVSNILDNYRLQLKVQPSGRRLLGTTVVDRDGFPTWLTGADR 240
           DGLDVAGKLTSNALAIVTAVSNILDNYRLQLKVQPSGRRLLGTTVVDRDGFPTWLTGADR
Sbjct: 181 DGLDVAGKLTSNALAIVTAVSNILDNYRLQLKVQPSGRRLLGTTVVDRDGFPTWLTGADR 240

Query: 241 KLLASKQGGVRPTPNAVVAKDGSGKYKTIAAALAAYPKVLRGRYVIYVKAGIYDEYITLT 300
           KLLASKQ GVRPTPNAVVAKDGSGKYKTIAAALAAYPKVLRGRYVIYVKAGIYDEYITLT
Sbjct: 241 KLLASKQRGVRPTPNAVVAKDGSGKYKTIAAALAAYPKVLRGRYVIYVKAGIYDEYITLT 300

Query: 301 KDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQ 360
           KDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQ
Sbjct: 301 KDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQ 360

Query: 361 AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVVSGTVDFIFGDSSTVIQNSLII 420
           AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVVSGTVDFIFGDSSTVIQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVVSGTVDFIFGDSSTVIQNSLII 420

Query: 421 VRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480
           VRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART
Sbjct: 421 VRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480

Query: 481 VIMESTLGDFIQPAGYMPWSGDFALETCLYLEYGNRGPGANTNRRVRWKGAKVIGRNEAL 540
           VIMESTLGDFIQPAGYMPWSGDFALETCLYLEYGNRGPGANTNRRVRWKGAKVIGRNEAL
Sbjct: 481 VIMESTLGDFIQPAGYMPWSGDFALETCLYLEYGNRGPGANTNRRVRWKGAKVIGRNEAL 540

Query: 541 QYTAGAFLLGRSWLPTTGGLYYLGLKN 568
           QYTAGAFLLGRSWLPTTGGLYYLGLKN
Sbjct: 541 QYTAGAFLLGRSWLPTTGGLYYLGLKN 567

BLAST of CSPI05G02400 vs. TrEMBL
Match: A0A0A0KLN2_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_5G119700 PE=4 SV=1)

HSP 1 Score: 938.7 bits (2425), Expect = 3.3e-270
Identity = 461/567 (81.31%), Postives = 517/567 (91.18%), Query Frame = 1

Query: 1   MLGKVIVSGLSLILVVGIALAIAATINKSNASNGGTEDVSPKMKAVSAICSTTDYQDECK 60
           M+GKV++SG+SLILVVG+ALA+ A +NKS++S+  T ++SPKMKAV++ICS  DYQ EC+
Sbjct: 1   MVGKVVMSGISLILVVGVALAVVAIVNKSDSSDE-TANLSPKMKAVASICSNADYQPECQ 60

Query: 61  TTLDHVARNTSSNDPKDYAEAAILATIGEITKGYNLSDSLIVEASTNASIKMSVEDCKDL 120
           TTL  V  N+S  DPK++ +AAI +TI E+ KGYNL+D+L+VEA+ NA+IKMSV+DCKDL
Sbjct: 61  TTLGSVGNNSSVEDPKEFIKAAITSTIEEMKKGYNLTDNLMVEAANNATIKMSVDDCKDL 120

Query: 121 LQFAIDELQASYSAVGESDLHTDADRVADIKNWLSAVISYQQSCLDGLGEFDPQLKQRMQ 180
           LQ AIDEL ASYS VG+ DLHT+ DR+ADIKNWL+AVISYQQSCLDGL EFDPQLKQ+MQ
Sbjct: 121 LQSAIDELHASYSTVGDPDLHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQ 180

Query: 181 DGLDVAGKLTSNALAIVTAVSNILDNYRLQLKVQPSGRRLLGTTVVDRDGFPTWLTGADR 240
           DGLDVAGKLTSNALAIV AVS+IL    LQLKVQPSGRRLLGTT VD D FPTWLTG+DR
Sbjct: 181 DGLDVAGKLTSNALAIVGAVSDILKELGLQLKVQPSGRRLLGTTEVDSDSFPTWLTGSDR 240

Query: 241 KLLASKQGGVRPTPNAVVAKDGSGKYKTIAAALAAYPKVLRGRYVIYVKAGIYDEYITLT 300
           KLLA+K+GGVR  PN VVAKDGSG+YKTI AALAAYPK L+GRYVIYVKAG+Y+E I +T
Sbjct: 241 KLLAAKRGGVRVKPNVVVAKDGSGQYKTIGAALAAYPKALKGRYVIYVKAGVYNEPIIVT 300

Query: 301 KDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQ 360
           KDMKN+FMYGDGPRKTIVTGRKSNRDG TTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQ
Sbjct: 301 KDMKNIFMYGDGPRKTIVTGRKSNRDGITTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQ 360

Query: 361 AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVVSGTVDFIFGDSSTVIQNSLII 420
           AVALRVQSDRSAFFNCRMDG+QDTLYVQTHRQFYRNCV+SGTVDFIFGDS+T+IQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSNTIIQNSLII 420

Query: 421 VRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480
           VRRPMDNQQNTVTA GRA++KEI+GLVIHNCRIVPEQKLFAERFKIPTFLGRPWK+YART
Sbjct: 421 VRRPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYART 480

Query: 481 VIMESTLGDFIQPAGYMPWSGDFALETCLYLEYGNRGPGANTNRRVRWKGAKVIGRNEAL 540
           VIMESTLGDFIQPAGYMPWSG+FAL TC Y EYGNRGPGANTNRRVRWKG +VIGRNEA+
Sbjct: 481 VIMESTLGDFIQPAGYMPWSGNFALATCSYFEYGNRGPGANTNRRVRWKGVRVIGRNEAM 540

Query: 541 QYTAGAFLLGRSWLPTTGGLYYLGLKN 568
           Q+TAG FLLG++WLP TGG Y LGLKN
Sbjct: 541 QFTAGPFLLGKAWLPGTGGPYLLGLKN 566

BLAST of CSPI05G02400 vs. TrEMBL
Match: A5ATJ7_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_040170 PE=4 SV=1)

HSP 1 Score: 708.0 bits (1826), Expect = 9.4e-201
Identity = 359/546 (65.75%), Postives = 435/546 (79.67%), Query Frame = 1

Query: 1   MLGKVIVSGLSLILVVGIALAIAATINKSNAS-NGGTEDVSPKMKAVSAICSTTDYQDEC 60
           M+GK +VSG+SLILVVG+ + I +      +  N G  ++S  MKAV+++C+T DY+D C
Sbjct: 1   MIGKAVVSGISLILVVGVIIGIVSVTRPHGSDRNDGNTNMSSSMKAVASVCATADYKDAC 60

Query: 61  KTTLDHVARNTSSNDPKDYAEAAILATIGEITKGYNLSDSLIVEASTNASIKMSVEDCKD 120
             TL  VA+N S+  PKDY +AA+  T+ EI    NLS+ L V+A+ ++  +M++ DCKD
Sbjct: 61  MQTLSPVAKNGSAT-PKDYIQAAVQVTMKEIKSSMNLSEKL-VQATNDSRTQMALGDCKD 120

Query: 121 LLQFAIDELQASYSAVGESDLHTDADRVADIKNWLSAVISYQQSCLDGLGEFDPQLKQRM 180
           LLQFAIDELQ S+S+VGESDL T      +I NWLSAV+SYQQ+CLDG+   +P+ +  M
Sbjct: 121 LLQFAIDELQESFSSVGESDLQTLDQLSTEIMNWLSAVVSYQQTCLDGV--IEPRFQTAM 180

Query: 181 QDGLDVAGKLTSNALAIVTAVSNILDNYRLQLKVQPSGRRLLGTT-VVDRDGFPTWLTGA 240
           Q GL  A +LTSNALAIV+ +S IL  + + L ++P+ RRLLG   V+  DG+PTW +  
Sbjct: 181 QKGLLNATQLTSNALAIVSDISQILTKFNVSLDLKPNSRRLLGEIDVLGHDGYPTWFSAT 240

Query: 241 DRKLLASKQGGVRPTPNAVVAKDGSGKYKTIAAALAAYPKVLRGRYVIYVKAGIYDEYIT 300
           DRKLLAS   G R TPNA+VAKDGSG + TIAAALAAYPK L+GRYVIYVKAGIY EYIT
Sbjct: 241 DRKLLASHDNG-RLTPNAIVAKDGSGHFTTIAAALAAYPKNLKGRYVIYVKAGIYREYIT 300

Query: 301 LTKDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPEG 360
           +TKD  NV+MYGDGPRKTIVTG KS RDG TT  TA+F+AIG+GF+ +SMGF NTAGP+G
Sbjct: 301 VTKDQVNVYMYGDGPRKTIVTGTKSYRDGITTYKTATFSAIGKGFVARSMGFVNTAGPDG 360

Query: 361 HQAVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVVSGTVDFIFGDSSTVIQNSL 420
           HQAVALRVQSD SA FNCRMDG+QDTLY+Q HRQFYRNCV+SGT+DFIFGDS+TVIQNSL
Sbjct: 361 HQAVALRVQSDMSAIFNCRMDGYQDTLYIQAHRQFYRNCVISGTIDFIFGDSTTVIQNSL 420

Query: 421 IIVRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYA 480
           IIVRRP DNQQNTVTA G+AE++E TGLVIHNCRIVPEQKLF +RFKIP+FLGRPWK Y+
Sbjct: 421 IIVRRPKDNQQNTVTAHGKAEKRETTGLVIHNCRIVPEQKLFPDRFKIPSFLGRPWKPYS 480

Query: 481 RTVIMESTLGDFIQPAGYMPWSGDFALETCLYLEYGNRGPGANTNRRVRWKGAKVI-GRN 540
           +T+IME+TLGDFIQPAG+MPW+GDFAL T  Y EYGNRGPGANT  RV WKG ++I  RN
Sbjct: 481 KTIIMETTLGDFIQPAGWMPWAGDFALNTLFYAEYGNRGPGANTRSRVTWKGYRIIKTRN 540

Query: 541 EALQYT 544
           EALQYT
Sbjct: 541 EALQYT 541

BLAST of CSPI05G02400 vs. TrEMBL
Match: F6HZG5_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_07s0005g02750 PE=4 SV=1)

HSP 1 Score: 699.5 bits (1804), Expect = 3.3e-198
Identity = 357/569 (62.74%), Postives = 436/569 (76.63%), Query Frame = 1

Query: 1   MLGKVIVSGLSLILVVGIALAIAATINKSNASN-GGTEDVSPKMKAVSAICSTTDYQDEC 60
           M+GK +VSG+SL LVVG+ + I +      +    G  ++S  MKAV+++C+T DY+D C
Sbjct: 249 MIGKAVVSGISLFLVVGVIIGIVSVTRPHGSDRTDGDTNMSSSMKAVASVCATADYKDAC 308

Query: 61  KTTLDHVARNTSSNDPKDYAEAAILATIGEITKGYNLSDSLIVEASTNASIKMSVEDCKD 120
             TL  V +N SS  PKDY +AA+  TI +I    NLS+ L  +A+ ++  +M++ DCKD
Sbjct: 309 MQTLSPVPKNGSSATPKDYIQAAVQVTIKQIKSSMNLSEKLF-QATNDSRTQMALGDCKD 368

Query: 121 LLQFAIDELQASYSAVGESDLHTDADRVADIKNWLSAVISYQQSCLDGLGEFDPQLKQRM 180
           LLQFAIDELQ S+S+VGESDL T      +I NWLSA +SYQQ+CLDG+   +P+ +  M
Sbjct: 369 LLQFAIDELQESFSSVGESDLQTLDQLSTEIMNWLSAAVSYQQTCLDGV--IEPRFQAAM 428

Query: 181 QDGLDVAGKLTSNALAIVTAVSNILDNYRLQLKVQPSGRRLLGTT-VVDRDGFPTWLTGA 240
           Q GL  A +LTSNALAIV+ +S IL  + + L ++P+ RRLLG   V+  DG+PTW +  
Sbjct: 429 QKGLLNATQLTSNALAIVSDLSQILTKFNVPLDLKPNSRRLLGEIEVLGHDGYPTWFSAT 488

Query: 241 DRKLLASKQGGVRPTPNAVVAKDGSGKYKTIAAALAAYPKVLRGRYVIYVKAGIYDEYIT 300
           DRKLLA +  G R TPNA+VAKDGSG + TIAAALAAYPK L+GRYVIYVKAGIY EYIT
Sbjct: 489 DRKLLALQDNG-RLTPNAIVAKDGSGHFTTIAAALAAYPKNLKGRYVIYVKAGIYREYIT 548

Query: 301 LTKDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPEG 360
           +TKD  NV+MYGDGPRKTIVTG K  RDG TT  TA+F+AIG+GF+ +SMGF NTAGP+G
Sbjct: 549 VTKDHVNVYMYGDGPRKTIVTGTKCYRDGITTYKTATFSAIGKGFVARSMGFVNTAGPDG 608

Query: 361 HQAVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVVSGTVDFIFGDSSTVIQNSL 420
           HQAVALRVQSD SAFFNCRMDG+QDTLYVQ HRQFYRNCV+SGT+DFIFGDS+TVIQNSL
Sbjct: 609 HQAVALRVQSDMSAFFNCRMDGYQDTLYVQAHRQFYRNCVISGTIDFIFGDSTTVIQNSL 668

Query: 421 IIVRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYA 480
           IIVRRP D QQNTVTAQG+ E++E TGLVIH+CRIVPEQKLF +RFKIP+FLGRPWK Y+
Sbjct: 669 IIVRRPNDKQQNTVTAQGKTEKRETTGLVIHDCRIVPEQKLFPDRFKIPSFLGRPWKPYS 728

Query: 481 RTVIMESTLGDFIQPAGYMPWSGDFALETCLYLEYGNRGPGANTNRRVRWKGAKVI-GRN 540
           +T+IME+TLGDFIQPAG+ PW+G F   T LY EYGN GPGANT+ RV WKG ++I  RN
Sbjct: 729 KTIIMETTLGDFIQPAGWTPWAGKFVPNTLLYAEYGNLGPGANTHSRVTWKGYRIIKTRN 788

Query: 541 EALQYTAGAFLLGRSWLPTTGGLYYLGLK 567
           EALQYT  +F+ G  WL      Y  GLK
Sbjct: 789 EALQYTVNSFIQGNLWLKQINIPYLPGLK 813

BLAST of CSPI05G02400 vs. TrEMBL
Match: F6HE41_VITVI (Pectinesterase OS=Vitis vinifera GN=VIT_05s0020g00800 PE=4 SV=1)

HSP 1 Score: 688.0 bits (1774), Expect = 1.0e-194
Identity = 351/569 (61.69%), Postives = 439/569 (77.15%), Query Frame = 1

Query: 3   GKVIVSGLSLILVVGIALAIAATINKSN---ASNGGTEDV-SPKMKAVSAICSTTDYQDE 62
           GKV V G+S+ILVVG+A+ + A ++      +++G  E V S   KAV+AICS TDY+ +
Sbjct: 4   GKVAVGGISIILVVGVAIGLIAGLHGRGGHKSTDGDDEKVLSTTTKAVAAICSPTDYKQQ 63

Query: 63  CKTTLDHVARNTSSNDPKDYAEAAILATIGEITKGYNLSDSLIVEASTNASIKMSVEDCK 122
           C ++   +A N S+  PKD+  AAI  T+ E+ +    S+S+ +  +++   KM+ EDC+
Sbjct: 64  CISSFQTLANNHSAT-PKDFLMAAIDITMKEVKEAIGKSESIGLAGNSSGRQKMATEDCQ 123

Query: 123 DLLQFAIDELQASYSAVGESDLHTDADRVADIKNWLSAVISYQQSCLDGLGEFDPQLKQR 182
           DLLQ AI ELQAS+S VG++ +HT  DR  D+KNWLSAVISYQQSC+DG  E  P++K  
Sbjct: 124 DLLQLAIGELQASFSMVGDAAMHTINDRAQDLKNWLSAVISYQQSCMDGFDE-TPEVKSA 183

Query: 183 MQDGLDVAGKLTSNALAIVTAVSNILDNYRLQLKVQPSGRRLLGTTVVDRDGFPTWLTGA 242
           +Q+GL  A +LTSNALAIV+ +S IL ++ + L    S RRL   T  + D +P+W + A
Sbjct: 184 IQNGLLNATQLTSNALAIVSEISAILTSFNIPLNFTSSFRRLQEATE-ESDQYPSWFSAA 243

Query: 243 DRKLLASKQGGVRPTPNAVVAKDGSGKYKTIAAALAAYPKVLRGRYVIYVKAGIYDEYIT 302
           DRKLL     G + TPNAVVA DGSG+YK+I AALAAYPK L GRYVIYVKAGIYDEYIT
Sbjct: 244 DRKLLGRVDNG-KVTPNAVVALDGSGQYKSIGAALAAYPKNLNGRYVIYVKAGIYDEYIT 303

Query: 303 LTKDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPEG 362
           + K++ N+FMYGDGPRKT+VTG+KS  DG TT  T++F+ IG GF+CKSMGF NTAGPEG
Sbjct: 304 IEKNLVNIFMYGDGPRKTMVTGKKSFLDGITTYKTSTFSVIGNGFICKSMGFRNTAGPEG 363

Query: 363 HQAVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVVSGTVDFIFGDSSTVIQNSL 422
           HQAVALRVQSD SAFFNCRMDG+QDTLYVQ HRQFYRNCV+SGTVDFIFGDS+T+IQNSL
Sbjct: 364 HQAVALRVQSDYSAFFNCRMDGYQDTLYVQAHRQFYRNCVISGTVDFIFGDSTTLIQNSL 423

Query: 423 IIVRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYA 482
           IIVR+P+DNQQNT+TA GRAE++E TGLVI NCRIVPE+KLF  RFK P++LGRPWK Y+
Sbjct: 424 IIVRKPLDNQQNTITAHGRAEKRETTGLVIQNCRIVPEEKLFPTRFKTPSYLGRPWKAYS 483

Query: 483 RTVIMESTLGDFIQPAGYMPWSGDFALETCLYLEYGNRGPGANTNRRVRWKGAKVI-GRN 542
           RTV+MEST+GDFI PAG++PW G+FAL+T  Y EY N+GPGANT +RV+WKG KVI  RN
Sbjct: 484 RTVVMESTMGDFIHPAGWLPWMGNFALDTLYYAEYANKGPGANTAQRVKWKGYKVITNRN 543

Query: 543 EALQYTAGAFLLGRSWLPTTGGLYYLGLK 567
           EALQ+TAG F+ G  WL  TG  Y+LG +
Sbjct: 544 EALQFTAGPFIQGNEWLRLTGAPYFLGFR 568

BLAST of CSPI05G02400 vs. TAIR10
Match: AT5G27870.1 (AT5G27870.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 444.9 bits (1143), Expect = 7.5e-125
Identity = 221/562 (39.32%), Postives = 348/562 (61.92%), Query Frame = 1

Query: 4   KVIVSGLSLILVVGIALAIAATINKSNASNGGTEDVSPKMKAVSAICSTTDYQDECKTTL 63
           + ++  +S +L++ + +A+   ++ + + N G E+++  +KA+  +C+ TDY++ C+ TL
Sbjct: 15  RYVIISISSVLLISMVVAVTIGVSVNKSDNAGDEEITTSVKAIKDVCAPTDYKETCEDTL 74

Query: 64  DHVARNTSSNDPKDYAEAAILATIGEITKGYNLSDSLIVEASTNASIKMSVEDCKDLLQF 123
              A++TS  DP +  + A  AT+ +I+     S ++I E   +   KM+++ CK+L+ +
Sbjct: 75  RKDAKDTS--DPLELVKTAFNATMKQISDVAKKSQTMI-ELQKDPRAKMALDQCKELMDY 134

Query: 124 AIDELQASYSAVGESDLHTDADRVADIKNWLSAVISYQQSCLDGLGEFDPQLKQRMQDGL 183
           AI EL  S+  +G+ + H   + +  ++ WLSA IS++Q+CLDG         + ++  L
Sbjct: 135 AIGELSKSFEELGKFEFHKVDEALVKLRIWLSATISHEQTCLDGFQGTQGNAGETIKKAL 194

Query: 184 DVAGKLTSNALAIVTAVSNILDNYRLQLKVQPSGRRLLGTTVVDRDGFPTWLTGADRKLL 243
             A +LT N LA+VT +SN L   ++    + + RRLL         FP+W+    R+LL
Sbjct: 195 KTAVQLTHNGLAMVTEMSNYLGQMQIP---EMNSRRLLS------QEFPSWMDARARRLL 254

Query: 244 ASKQGGVRPTPNAVVAKDGSGKYKTIAAALAAYPKVLRGRYVIYVKAGIYDEYITLTKDM 303
            +    V+  P+ VVA+DGSG+YKTI  AL   PK     +V+++K GIY EY+ + + M
Sbjct: 255 NAPMSEVK--PDIVVAQDGSGQYKTINEALNFVPKKKNTTFVVHIKEGIYKEYVQVNRSM 314

Query: 304 KNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQAVA 363
            ++   GDGP KT+++G KS +DG TT  TA+ A +G+ F+ K++ F NTAG   HQAVA
Sbjct: 315 THLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAFENTAGAIKHQAVA 374

Query: 364 LRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVVSGTVDFIFGDSSTVIQNSLIIVRR 423
           +RV +D S F+NC+ DG+QDTLY  +HRQFYR+C +SGT+DF+FGD++ V QN  ++VR+
Sbjct: 375 IRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAAAVFQNCTLLVRK 434

Query: 424 PMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYARTVIM 483
           P+ NQ   +TA GR + +E TG V+  C IV E    A + +  T+LGRPWK+Y+RT+IM
Sbjct: 435 PLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYLGRPWKEYSRTIIM 494

Query: 484 ESTLGDFIQPAGYMPWSGDFALETCLYLEYGNRGPGANTNRRVRWKGAKVIGRNEALQYT 543
            + + DF+ P G+ PW G+F L T  Y E  N GPGA   +RV W G K +   E L++T
Sbjct: 495 NTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPGIKKLSDEEILKFT 554

Query: 544 AGAFLLGRSWLPTTGGLYYLGL 566
              ++ G +W+P  G  Y LGL
Sbjct: 555 PAQYIQGDAWIPGKGVPYILGL 562

BLAST of CSPI05G02400 vs. TAIR10
Match: AT3G05610.1 (AT3G05610.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 442.6 bits (1137), Expect = 3.7e-124
Identity = 223/561 (39.75%), Postives = 343/561 (61.14%), Query Frame = 1

Query: 5   VIVSGLSLILVVGIALAIAATINKSNASNGGTEDVSPKMKAVSAICSTTDYQDECKTTLD 64
           + +S + LI +V +A+ +  ++NK +  + G  +V+  +KAV  +C+ TDY+  C+ TL 
Sbjct: 18  ITISSVLLISMV-VAVTVGVSLNKHDGDSKGKAEVNASVKAVKDVCAPTDYRKTCEDTLI 77

Query: 65  HVARNTSSNDPKDYAEAAILATIGEITKGYNLSDSLIVEASTNASIKMSVEDCKDLLQFA 124
              +NT+  DP +  + A   T+ +IT     S + I+E   ++  +M+++ CK+L+ +A
Sbjct: 78  KNGKNTT--DPMELVKTAFNVTMKQITDAAKKSQT-IMELQKDSRTRMALDQCKELMDYA 137

Query: 125 IDELQASYSAVGESDLHTDADRVADIKNWLSAVISYQQSCLDGLGEFDPQLKQRMQDGLD 184
           +DEL  S+  +G+ + H   + + +++ WLSA IS++++CL+G         + M+  L 
Sbjct: 138 LDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCLEGFQGTQGNAGETMKKALK 197

Query: 185 VAGKLTSNALAIVTAVSNILDNYRLQLKVQPSGRRLLGTTVVDRDGFPTWLTGADRKLLA 244
            A +LT N LAI++ +SN +   ++      + RRLL       +GFP+W+    RKLL 
Sbjct: 198 TAIELTHNGLAIISEMSNFVGQMQIP---GLNSRRLLA------EGFPSWVDQRGRKLLQ 257

Query: 245 SKQGGVRPTPNAVVAKDGSGKYKTIAAALAAYPKVLRGRYVIYVKAGIYDEYITLTKDMK 304
           +        P+ VVA+DGSG+YKTI  AL   PK     +V+++KAG+Y EY+ + K M 
Sbjct: 258 AAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIKAGLYKEYVQVNKTMS 317

Query: 305 NVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQAVAL 364
           ++   GDGP KTI++G K+ +DG TT  TA+ A +G  F+ K++GF NTAG   HQAVA+
Sbjct: 318 HLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIGFENTAGAIKHQAVAV 377

Query: 365 RVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVVSGTVDFIFGDSSTVIQNSLIIVRRP 424
           RVQSD S FFNCR DG+QDTLY  +HRQF+R+C +SGT+DF+FGD++ V QN  ++VR+P
Sbjct: 378 RVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDAAAVFQNCTLLVRKP 437

Query: 425 MDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYARTVIME 484
           + NQ   +TA GR + +E TG V   C I  E    A +     +LGRPWK+Y+RT+IM 
Sbjct: 438 LPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAYLGRPWKEYSRTIIMN 497

Query: 485 STLGDFIQPAGYMPWSGDFALETCLYLEYGNRGPGANTNRRVRWKGAKVIGRNEALQYTA 544
           + + DF+QP G+ PW GDF L+T  Y E  N GPG+    RV W G K +   + L++T 
Sbjct: 498 TFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWAGIKTLSEEDILKFTP 557

Query: 545 GAFLLGRSWLPTTGGLYYLGL 566
             ++ G  W+P  G  Y  GL
Sbjct: 558 AQYIQGDDWIPGKGVPYTTGL 565

BLAST of CSPI05G02400 vs. TAIR10
Match: AT2G47030.1 (AT2G47030.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 427.9 bits (1099), Expect = 9.5e-120
Identity = 255/604 (42.22%), Postives = 358/604 (59.27%), Query Frame = 1

Query: 1   MLGKVIVSGLSLILVVGIALAIAATINKSNASNGGTEDVSPKMKAVSAICSTTDYQDECK 60
           M+GKV+VS  S++L+VG+A+ + A INK+     G  ++SP+MKAV  IC +T  +  C 
Sbjct: 1   MIGKVVVSVASILLIVGVAIGVVAFINKN-----GDANLSPQMKAVQGICQSTSDKASCV 60

Query: 61  TTLDHVARNTSSNDPKDYAEAAILATIGEITKGYNLSDSLIVE--ASTNASIKMSVEDCK 120
            TL+ V     S DP    +A +LAT  E+TK  N +    V   +S + + K  ++ CK
Sbjct: 61  KTLEPV----KSEDPNKLIKAFMLATKDELTKSSNFTGQTEVNMGSSISPNNKAVLDYCK 120

Query: 121 DLLQFAIDELQASYSAVGESDLHTDADRVADIKNWLSAVISYQQSCLDGLGEFDPQLKQR 180
            +  +A+++L      +GE DL     ++  +K WL  V +YQ  CLD + E D  L++ 
Sbjct: 121 RVFMYALEDLATIIEEMGE-DLSQIGSKIDQLKQWLIGVYNYQTDCLDDIEEDD--LRKA 180

Query: 181 MQDGLDVAGKLTSNALAI-------VTAVSNILDNYRLQLKVQPS--------------- 240
           + +G+  +  LT+NA+ I       +  ++N +D+ +      P+               
Sbjct: 181 IGEGIANSKILTTNAIDIFHTVVSAMAKINNKVDDLKNMTGGIPTPGAPPVVDESPVADP 240

Query: 241 ---GRRLLGTTVVDRDGFPTWLTGADRKLLA--------SKQGGVRPTPNAVVAKDGSGK 300
               RRLL    +D  G PTW++GADRKL+A         + GG R   N VVAKDGSG+
Sbjct: 241 DGPARRLLED--IDETGIPTWVSGADRKLMAKAGRGRRGGRGGGARVRTNFVVAKDGSGQ 300

Query: 301 YKTIAAALAAYPKVLRGRYVIYVKAGIYDEYITLTKDMKNVFMYGDGPRKTIVTGRKS-- 360
           +KT+  A+ A P+  RGR +IY+KAG+Y E + + K   N+FM+GDG RKT+++  +S  
Sbjct: 301 FKTVQQAVDACPENNRGRCIIYIKAGLYREQVIIPKKKNNIFMFGDGARKTVISYNRSVA 360

Query: 361 NRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQAVALRVQSDRSAFFNCRMDGHQD 420
              G TT  +A+     EGF+ K MGF NTAGP GHQA A+RV  DR+  FNCR DG+QD
Sbjct: 361 LSRGTTTSLSATVQVESEGFMAKWMGFKNTAGPMGHQAAAIRVNGDRAVIFNCRFDGYQD 420

Query: 421 TLYVQTHRQFYRNCVVSGTVDFIFGDSSTVIQNSLIIVRRPMDNQQNTVTAQGRAEQKEI 480
           TLYV   RQFYRNCVVSGTVDFIFG S+TVIQN+LI+VR+    Q NTVTA G      +
Sbjct: 421 TLYVNNGRQFYRNCVVSGTVDFIFGKSATVIQNTLIVVRKGSKGQYNTVTADGNELGLGM 480

Query: 481 -TGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYARTVIMESTLGDFIQPAGYMPWSGD 540
             G+V+ NCRIVP++KL  ER  + T+LGRPWK+++ TVIM + +GD I+P G+  W G+
Sbjct: 481 KIGIVLQNCRIVPDRKLTPERLTVATYLGRPWKKFSTTVIMSTEMGDLIRPEGWKIWDGE 540

Query: 541 FALETCLYLEYGNRGPGANTNRRVRWKGAKVI-GRNEALQYTAGAFLLGRSWL-----PT 561
              ++C Y+EY NRGPGA  NRRV W  AKV     E   +TA  +L   +W+     P 
Sbjct: 541 SFHKSCRYVEYNNRGPGAFANRRVNW--AKVARSAAEVNGFTAANWLGPINWIQEANVPV 588

BLAST of CSPI05G02400 vs. TAIR10
Match: AT4G33230.1 (AT4G33230.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 427.9 bits (1099), Expect = 9.5e-120
Identity = 248/600 (41.33%), Postives = 354/600 (59.00%), Query Frame = 1

Query: 4   KVIVSGLSLILVVGIALAIAA----------------TINKSNASNGGTEDVSPKM---- 63
           K I+ G+  +LVV  A+   A                T NKS  S   +E  SPK     
Sbjct: 23  KRIILGVVSVLVVAAAIIGGAFAYVTYENKTQEQGKTTNNKSKDSPTKSESPSPKPPSSA 82

Query: 64  ----------KAVSAICSTTDYQDECKTTL-DHVARNTSSNDPKDYAEAAILATIGEITK 123
                     K +  +C++T Y+  C+ TL +   ++T   DP+   ++AI+A   ++ +
Sbjct: 83  AQTVKAGQVDKIIQTLCNSTLYKPTCQNTLKNETKKDTPQTDPRSLLKSAIVAVNDDLDQ 142

Query: 124 GYNLSDSLIVEASTNASIKMSVEDCKDLLQFAIDELQASYSAVGESDLHTDADRVADIKN 183
            +    SL  E   N   K ++  CK L+  A +EL  S   + +S+++  A  V D+ +
Sbjct: 143 VFKRVLSLKTE---NKDDKDAIAQCKLLVDEAKEELGTSMKRINDSEVNNFAKIVPDLDS 202

Query: 184 WLSAVISYQQSCLDGLGEFDPQLKQRMQDGLDVAGKLTSNALAIVTAVSNILDNY----- 243
           WLSAV+SYQ++C+DG  E   +LK  ++   + +  LTSN+LA++ ++   L +      
Sbjct: 203 WLSAVMSYQETCVDGFEE--GKLKTEIRKNFNSSQVLTSNSLAMIKSLDGYLSSVPKVKT 262

Query: 244 RLQLKVQPSGRRLLGTTVVDRDGFPTWLTGADRKLLASKQGGVRPT-PNAVVAKDGSGKY 303
           RL L+ + S +        + D   +WL+  +R++L  K   V+   PNA VAKDGSG +
Sbjct: 263 RLLLEARSSAK--------ETDHITSWLSNKERRML--KAVDVKALKPNATVAKDGSGNF 322

Query: 304 KTIAAALAAYPKVLRGRYVIYVKAGIYDEYITLTKDMKNVFMYGDGPRKTIVTGRKSNRD 363
            TI AAL A P   +GRY IY+K GIYDE + + K   NV M GDG +KTIVTG KS+  
Sbjct: 323 TTINAALKAMPAKYQGRYTIYIKHGIYDESVIIDKKKPNVTMVGDGSQKTIVTGNKSHAK 382

Query: 364 GFTTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQAVALRVQSDRSAFFNCRMDGHQDTLY 423
              T  TA+F A GEGF+ +SMGF NTAGPEGHQAVA+RVQSDRS F NCR +G+QDTLY
Sbjct: 383 KIRTFLTATFVAQGEGFMAQSMGFRNTAGPEGHQAVAIRVQSDRSVFLNCRFEGYQDTLY 442

Query: 424 VQTHRQFYRNCVVSGTVDFIFGDSSTVIQNSLIIVRRPMDNQQNTVTAQGRAEQKEITGL 483
             THRQ+YR+CV+ GTVDFIFGD++ + QN  I +R+ +  Q+NTVTAQGR ++ + TG 
Sbjct: 443 AYTHRQYYRSCVIIGTVDFIFGDAAAIFQNCDIFIRKGLPGQKNTVTAQGRVDKFQTTGF 502

Query: 484 VIHNCRIVPEQKLFAERFKIPTFLGRPWKQYARTVIMESTLGDFIQPAGYMPW-SGDFAL 543
           VIHNC + P + L   + +  ++LGRPWK ++RTV+MEST+ D I P G++ W   DFA+
Sbjct: 503 VIHNCTVAPNEDLKPVKAQFKSYLGRPWKPHSRTVVMESTIEDVIDPVGWLRWQETDFAI 562

Query: 544 ETCLYLEYGNRGPGANTNRRVRWKGAKVIGRNEALQYTAGAFLLGRSWLPTTGGLYYLGL 566
           +T  Y EY N GP   T  RV+W G +V+ + EA+++T G FL G  W+   G    LGL
Sbjct: 563 DTLSYAEYKNDGPSGATAARVKWPGFRVLNKEEAMKFTVGPFLQG-EWIQAIGSPVKLGL 606

BLAST of CSPI05G02400 vs. TAIR10
Match: AT2G26450.1 (AT2G26450.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 425.2 bits (1092), Expect = 6.1e-119
Identity = 231/524 (44.08%), Postives = 330/524 (62.98%), Query Frame = 1

Query: 44  KAVSAICSTTDYQDECKTTL-DHVARNTSSNDPKDYAEAAILATIGEITKGYNLSDSLIV 103
           K +  +CS+T Y   C+ TL +   +  + ++P  + ++AI A   ++        SL  
Sbjct: 107 KIIQTLCSSTLYMQICEKTLKNRTDKGFALDNPTTFLKSAIEAVNEDLDLVLEKVLSLKT 166

Query: 104 EASTNASIKMSVEDCKDLLQFAIDELQASYSAVGESDLHTDADRVADIKNWLSAVISYQQ 163
           E   N   K ++E CK L++ A +E  AS + +  +++++    V D+++WLSAV+SYQ+
Sbjct: 167 E---NQDDKDAIEQCKLLVEDAKEETVASLNKINVTEVNSFEKVVPDLESWLSAVMSYQE 226

Query: 164 SCLDGLGEFDPQLKQRMQDGLDVAGKLTSNALAIVTAVSNILDNYRLQLKVQPSGRRLLG 223
           +CLDG  E +  LK  ++  ++ +  LTSN+LA++   +   +N    +KV    R LL 
Sbjct: 227 TCLDGFEEGN--LKSEVKTSVNSSQVLTSNSLALIKTFT---ENLSPVMKVVE--RHLL- 286

Query: 224 TTVVDRDGFPTWLTGADRKLLASKQGGVRPTPNAVVAKDGSGKYKTIAAALAAYPKVLRG 283
                 D  P+W++  DR++L +        PNA VAKDGSG + TI  AL A P+   G
Sbjct: 287 ------DDIPSWVSNDDRRMLRAVDVKALK-PNATVAKDGSGDFTTINDALRAMPEKYEG 346

Query: 284 RYVIYVKAGIYDEYITLTKDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEG 343
           RY+IYVK GIYDEY+T+ K   N+ M GDG +KTIVTG KS+     T  TA+F A GEG
Sbjct: 347 RYIIYVKQGIYDEYVTVDKKKANLTMVGDGSQKTIVTGNKSHAKKIRTFLTATFVAQGEG 406

Query: 344 FLCKSMGFTNTAGPEGHQAVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVVSGT 403
           F+ +SMGF NTAGPEGHQAVA+RVQSDRS F NCR +G+QDTLY  THRQ+YR+CV+ GT
Sbjct: 407 FMAQSMGFRNTAGPEGHQAVAIRVQSDRSIFLNCRFEGYQDTLYAYTHRQYYRSCVIVGT 466

Query: 404 VDFIFGDSSTVIQNSLIIVRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAE 463
           +DFIFGD++ + QN  I +R+ +  Q+NTVTAQGR ++ + TG V+HNC+I   + L   
Sbjct: 467 IDFIFGDAAAIFQNCNIFIRKGLPGQKNTVTAQGRVDKFQTTGFVVHNCKIAANEDLKPV 526

Query: 464 RFKIPTFLGRPWKQYARTVIMESTLGDFIQPAGYMPW-SGDFALETCLYLEYGNRGPGAN 523
           + +  ++LGRPWK Y+RT+IMES + + I P G++ W   DFA++T  Y EY N+G   +
Sbjct: 527 KEEYKSYLGRPWKNYSRTIIMESKIENVIDPVGWLRWQETDFAIDTLYYAEYNNKGSSGD 586

Query: 524 TNRRVRWKGAKVIGRNEALQYTAGAFLLGRSWLPTTGGLYYLGL 566
           T  RV+W G KVI + EAL YT G FL G  W+  +G    LGL
Sbjct: 587 TTSRVKWPGFKVINKEEALNYTVGPFLQG-DWISASGSPVKLGL 611

BLAST of CSPI05G02400 vs. NCBI nr
Match: gi|449463555|ref|XP_004149499.1| (PREDICTED: pectinesterase-like [Cucumis sativus])

HSP 1 Score: 1122.8 bits (2903), Expect = 0.0e+00
Identity = 565/567 (99.65%), Postives = 566/567 (99.82%), Query Frame = 1

Query: 1   MLGKVIVSGLSLILVVGIALAIAATINKSNASNGGTEDVSPKMKAVSAICSTTDYQDECK 60
           MLGKVIVSGLSLILVVGIALAIAATINKSNASNGGTEDVSPKMKAVSAICSTTDYQDECK
Sbjct: 1   MLGKVIVSGLSLILVVGIALAIAATINKSNASNGGTEDVSPKMKAVSAICSTTDYQDECK 60

Query: 61  TTLDHVARNTSSNDPKDYAEAAILATIGEITKGYNLSDSLIVEASTNASIKMSVEDCKDL 120
           TTLDHVARNTSSNDPKDYAEAAILATIGEITKGYNLSDSLIVEASTNASIKMSVEDCKDL
Sbjct: 61  TTLDHVARNTSSNDPKDYAEAAILATIGEITKGYNLSDSLIVEASTNASIKMSVEDCKDL 120

Query: 121 LQFAIDELQASYSAVGESDLHTDADRVADIKNWLSAVISYQQSCLDGLGEFDPQLKQRMQ 180
           LQFAIDELQASYSAVGESDLHTD+DRVADIKNWLSAVISYQQSCLDGLGEFDPQLKQRMQ
Sbjct: 121 LQFAIDELQASYSAVGESDLHTDSDRVADIKNWLSAVISYQQSCLDGLGEFDPQLKQRMQ 180

Query: 181 DGLDVAGKLTSNALAIVTAVSNILDNYRLQLKVQPSGRRLLGTTVVDRDGFPTWLTGADR 240
           DGLDVAGKLTSNALAIVTAVSNILDNYRLQLKVQPSGRRLLGTTVVDRDGFPTWLTGADR
Sbjct: 181 DGLDVAGKLTSNALAIVTAVSNILDNYRLQLKVQPSGRRLLGTTVVDRDGFPTWLTGADR 240

Query: 241 KLLASKQGGVRPTPNAVVAKDGSGKYKTIAAALAAYPKVLRGRYVIYVKAGIYDEYITLT 300
           KLLASKQ GVRPTPNAVVAKDGSGKYKTIAAALAAYPKVLRGRYVIYVKAGIYDEYITLT
Sbjct: 241 KLLASKQRGVRPTPNAVVAKDGSGKYKTIAAALAAYPKVLRGRYVIYVKAGIYDEYITLT 300

Query: 301 KDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQ 360
           KDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQ
Sbjct: 301 KDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQ 360

Query: 361 AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVVSGTVDFIFGDSSTVIQNSLII 420
           AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVVSGTVDFIFGDSSTVIQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVVSGTVDFIFGDSSTVIQNSLII 420

Query: 421 VRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480
           VRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART
Sbjct: 421 VRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480

Query: 481 VIMESTLGDFIQPAGYMPWSGDFALETCLYLEYGNRGPGANTNRRVRWKGAKVIGRNEAL 540
           VIMESTLGDFIQPAGYMPWSGDFALETCLYLEYGNRGPGANTNRRVRWKGAKVIGRNEAL
Sbjct: 481 VIMESTLGDFIQPAGYMPWSGDFALETCLYLEYGNRGPGANTNRRVRWKGAKVIGRNEAL 540

Query: 541 QYTAGAFLLGRSWLPTTGGLYYLGLKN 568
           QYTAGAFLLGRSWLPTTGGLYYLGLKN
Sbjct: 541 QYTAGAFLLGRSWLPTTGGLYYLGLKN 567

BLAST of CSPI05G02400 vs. NCBI nr
Match: gi|659072607|ref|XP_008466403.1| (PREDICTED: pectinesterase-like [Cucumis melo])

HSP 1 Score: 1072.4 bits (2772), Expect = 2.7e-310
Identity = 531/567 (93.65%), Postives = 550/567 (97.00%), Query Frame = 1

Query: 1   MLGKVIVSGLSLILVVGIALAIAATINKSNASNGGTEDVSPKMKAVSAICSTTDYQDECK 60
           M+GKVIVS LSLILVVGIALA+ ATINK N SNGG E+VSPKMKA+SAICSTTDYQ+ECK
Sbjct: 1   MVGKVIVSALSLILVVGIALAVVATINKRNGSNGGKEEVSPKMKAISAICSTTDYQEECK 60

Query: 61  TTLDHVARNTSSNDPKDYAEAAILATIGEITKGYNLSDSLIVEASTNASIKMSVEDCKDL 120
            TL+HVA+NTSSNDPKDYA+AAILATI EITKGYNLSDSLIVEA  NASIKMSVEDC DL
Sbjct: 61  NTLNHVAQNTSSNDPKDYAKAAILATIKEITKGYNLSDSLIVEAGNNASIKMSVEDCNDL 120

Query: 121 LQFAIDELQASYSAVGESDLHTDADRVADIKNWLSAVISYQQSCLDGLGEFDPQLKQRMQ 180
           LQFAIDELQASYS VGESDLHTDADR+ADIKNWLSAV+SYQQSCLDGLGEFDPQLKQRMQ
Sbjct: 121 LQFAIDELQASYSTVGESDLHTDADRIADIKNWLSAVVSYQQSCLDGLGEFDPQLKQRMQ 180

Query: 181 DGLDVAGKLTSNALAIVTAVSNILDNYRLQLKVQPSGRRLLGTTVVDRDGFPTWLTGADR 240
           DGLDVAGKLTSNALAI+TAVSNILDNY LQLKVQPSGRRLLGTT VD DGFPTWLTGADR
Sbjct: 181 DGLDVAGKLTSNALAILTAVSNILDNYGLQLKVQPSGRRLLGTTEVDSDGFPTWLTGADR 240

Query: 241 KLLASKQGGVRPTPNAVVAKDGSGKYKTIAAALAAYPKVLRGRYVIYVKAGIYDEYITLT 300
           KLLASKQGGVRPTPNAVVAKDGSG+YKTIAAALAAYPK LRGRYVIYVKAGIYDEYIT+T
Sbjct: 241 KLLASKQGGVRPTPNAVVAKDGSGQYKTIAAALAAYPKALRGRYVIYVKAGIYDEYITVT 300

Query: 301 KDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQ 360
           KDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGP+GHQ
Sbjct: 301 KDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPQGHQ 360

Query: 361 AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVVSGTVDFIFGDSSTVIQNSLII 420
           AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCV+SGTVDFIFGDSST+IQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLII 420

Query: 421 VRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480
           VRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART
Sbjct: 421 VRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480

Query: 481 VIMESTLGDFIQPAGYMPWSGDFALETCLYLEYGNRGPGANTNRRVRWKGAKVIGRNEAL 540
           VIMESTLGDFIQPAGYMPWSG+FALETCLYLEYGNRGPGANTNRRVRWKG KVIGRNEAL
Sbjct: 481 VIMESTLGDFIQPAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVIGRNEAL 540

Query: 541 QYTAGAFLLGRSWLPTTGGLYYLGLKN 568
           QYTAGAFLLGR+WLP TGG+YYLGLKN
Sbjct: 541 QYTAGAFLLGRTWLPRTGGIYYLGLKN 567

BLAST of CSPI05G02400 vs. NCBI nr
Match: gi|778698533|ref|XP_004149498.2| (PREDICTED: pectinesterase-like [Cucumis sativus])

HSP 1 Score: 938.7 bits (2425), Expect = 4.7e-270
Identity = 461/567 (81.31%), Postives = 517/567 (91.18%), Query Frame = 1

Query: 1   MLGKVIVSGLSLILVVGIALAIAATINKSNASNGGTEDVSPKMKAVSAICSTTDYQDECK 60
           M+GKV++SG+SLILVVG+ALA+ A +NKS++S+  T ++SPKMKAV++ICS  DYQ EC+
Sbjct: 1   MVGKVVMSGISLILVVGVALAVVAIVNKSDSSDE-TANLSPKMKAVASICSNADYQPECQ 60

Query: 61  TTLDHVARNTSSNDPKDYAEAAILATIGEITKGYNLSDSLIVEASTNASIKMSVEDCKDL 120
           TTL  V  N+S  DPK++ +AAI +TI E+ KGYNL+D+L+VEA+ NA+IKMSV+DCKDL
Sbjct: 61  TTLGSVGNNSSVEDPKEFIKAAITSTIEEMKKGYNLTDNLMVEAANNATIKMSVDDCKDL 120

Query: 121 LQFAIDELQASYSAVGESDLHTDADRVADIKNWLSAVISYQQSCLDGLGEFDPQLKQRMQ 180
           LQ AIDEL ASYS VG+ DLHT+ DR+ADIKNWL+AVISYQQSCLDGL EFDPQLKQ+MQ
Sbjct: 121 LQSAIDELHASYSTVGDPDLHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQ 180

Query: 181 DGLDVAGKLTSNALAIVTAVSNILDNYRLQLKVQPSGRRLLGTTVVDRDGFPTWLTGADR 240
           DGLDVAGKLTSNALAIV AVS+IL    LQLKVQPSGRRLLGTT VD D FPTWLTG+DR
Sbjct: 181 DGLDVAGKLTSNALAIVGAVSDILKELGLQLKVQPSGRRLLGTTEVDSDSFPTWLTGSDR 240

Query: 241 KLLASKQGGVRPTPNAVVAKDGSGKYKTIAAALAAYPKVLRGRYVIYVKAGIYDEYITLT 300
           KLLA+K+GGVR  PN VVAKDGSG+YKTI AALAAYPK L+GRYVIYVKAG+Y+E I +T
Sbjct: 241 KLLAAKRGGVRVKPNVVVAKDGSGQYKTIGAALAAYPKALKGRYVIYVKAGVYNEPIIVT 300

Query: 301 KDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQ 360
           KDMKN+FMYGDGPRKTIVTGRKSNRDG TTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQ
Sbjct: 301 KDMKNIFMYGDGPRKTIVTGRKSNRDGITTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQ 360

Query: 361 AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVVSGTVDFIFGDSSTVIQNSLII 420
           AVALRVQSDRSAFFNCRMDG+QDTLYVQTHRQFYRNCV+SGTVDFIFGDS+T+IQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSNTIIQNSLII 420

Query: 421 VRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480
           VRRPMDNQQNTVTA GRA++KEI+GLVIHNCRIVPEQKLFAERFKIPTFLGRPWK+YART
Sbjct: 421 VRRPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYART 480

Query: 481 VIMESTLGDFIQPAGYMPWSGDFALETCLYLEYGNRGPGANTNRRVRWKGAKVIGRNEAL 540
           VIMESTLGDFIQPAGYMPWSG+FAL TC Y EYGNRGPGANTNRRVRWKG +VIGRNEA+
Sbjct: 481 VIMESTLGDFIQPAGYMPWSGNFALATCSYFEYGNRGPGANTNRRVRWKGVRVIGRNEAM 540

Query: 541 QYTAGAFLLGRSWLPTTGGLYYLGLKN 568
           Q+TAG FLLG++WLP TGG Y LGLKN
Sbjct: 541 QFTAGPFLLGKAWLPGTGGPYLLGLKN 566

BLAST of CSPI05G02400 vs. NCBI nr
Match: gi|659072603|ref|XP_008466382.1| (PREDICTED: pectinesterase-like isoform X2 [Cucumis melo])

HSP 1 Score: 933.3 bits (2411), Expect = 2.0e-268
Identity = 463/567 (81.66%), Postives = 516/567 (91.01%), Query Frame = 1

Query: 1   MLGKVIVSGLSLILVVGIALAIAATINKSNASNGGTEDVSPKMKAVSAICSTTDYQDECK 60
           M+ KV+++  SL LVVG+ LA+ A +NK+++S+  T D+SPKMKAVS+ICS TDYQ EC+
Sbjct: 1   MVAKVVMAVTSL-LVVGVVLAVFAMVNKNHSSDE-TADMSPKMKAVSSICSNTDYQQECQ 60

Query: 61  TTLDHVARNTSSNDPKDYAEAAILATIGEITKGYNLSDSLIVEASTNASIKMSVEDCKDL 120
           TTL +V  N+S +DPK++ +AAI ATI E+ KGYNL+D+L+VEA+ NASIKM+V+DC+DL
Sbjct: 61  TTLGNVGHNSSVDDPKEFLKAAITATIEEMKKGYNLTDNLMVEAANNASIKMTVDDCRDL 120

Query: 121 LQFAIDELQASYSAVGESDLHTDADRVADIKNWLSAVISYQQSCLDGLGEFDPQLKQRMQ 180
           LQ AIDEL ASYS VG+ DLHT+ DRVADIKNWL+AVISYQQSCLDGL EFDPQLKQ+MQ
Sbjct: 121 LQSAIDELHASYSTVGDPDLHTNEDRVADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQ 180

Query: 181 DGLDVAGKLTSNALAIVTAVSNILDNYRLQLKVQPSGRRLLGTTVVDRDGFPTWLTGADR 240
            GLDVA KLTSNALAIV AVS+IL +  LQLKVQPSGRRLLGTT VD DGFPTWLTGADR
Sbjct: 181 GGLDVAAKLTSNALAIVDAVSDILKSLGLQLKVQPSGRRLLGTTEVDNDGFPTWLTGADR 240

Query: 241 KLLASKQGGVRPTPNAVVAKDGSGKYKTIAAALAAYPKVLRGRYVIYVKAGIYDEYITLT 300
           KLLA+K+GGVR  PNAVVAKDGSG+YKTIAAALAAYPK L+GRYVIYVKAGIY+EYI +T
Sbjct: 241 KLLAAKRGGVRVKPNAVVAKDGSGQYKTIAAALAAYPKALKGRYVIYVKAGIYNEYIIVT 300

Query: 301 KDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQ 360
           KDMKNVFMYGDGPRKTIVTGRKSNRDG  TQNTA+FAAIGEGFLCKSMGFTNTAGPEGHQ
Sbjct: 301 KDMKNVFMYGDGPRKTIVTGRKSNRDGINTQNTATFAAIGEGFLCKSMGFTNTAGPEGHQ 360

Query: 361 AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVVSGTVDFIFGDSSTVIQNSLII 420
           AVALRVQSDRSAFFNCRMDG+QDTLYVQTHRQFYRNCVVSGTVDFIFGDS+T+IQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVVSGTVDFIFGDSNTIIQNSLII 420

Query: 421 VRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480
           VRRPMDNQQNTVTA GRA++KEI+GLVIHNCRIVPEQKLFAERFKIPTFLGRPWK+YART
Sbjct: 421 VRRPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYART 480

Query: 481 VIMESTLGDFIQPAGYMPWSGDFALETCLYLEYGNRGPGANTNRRVRWKGAKVIGRNEAL 540
           VIME+TLGDFIQPAGYMPWSGDFAL TC Y EYGNRGPGANT RRVRWKG +VIGRNEA+
Sbjct: 481 VIMETTLGDFIQPAGYMPWSGDFALATCSYFEYGNRGPGANTKRRVRWKGVRVIGRNEAM 540

Query: 541 QYTAGAFLLGRSWLPTTGGLYYLGLKN 568
           Q+TAG FLLGR+WLP TGG Y LGLKN
Sbjct: 541 QFTAGPFLLGRAWLPATGGPYLLGLKN 565

BLAST of CSPI05G02400 vs. NCBI nr
Match: gi|449467649|ref|XP_004151535.1| (PREDICTED: pectinesterase-like [Cucumis sativus])

HSP 1 Score: 932.9 bits (2410), Expect = 2.6e-268
Identity = 454/567 (80.07%), Postives = 514/567 (90.65%), Query Frame = 1

Query: 1   MLGKVIVSGLSLILVVGIALAIAATINKSNASNGGTEDVSPKMKAVSAICSTTDYQDECK 60
           M+ KV++ G+SL+LVVG+ALA+ A +N SN S+  TED+SPKMKAVS ICST +YQ+EC+
Sbjct: 1   MVNKVVLPGISLLLVVGVALAVVAIVN-SNKSSDATEDLSPKMKAVSTICSTANYQEECQ 60

Query: 61  TTLDHVARNTSSNDPKDYAEAAILATIGEITKGYNLSDSLIVEASTNASIKMSVEDCKDL 120
            TL + A N SS+DPK+Y +AAILATI E+ KGYNL+D  ++EA+ N SIKM VEDC+DL
Sbjct: 61  NTLTNAAHNASSDDPKEYVKAAILATIDEVKKGYNLTDGFLIEAANNRSIKMGVEDCRDL 120

Query: 121 LQFAIDELQASYSAVGESDLHTDADRVADIKNWLSAVISYQQSCLDGLGEFDPQLKQRMQ 180
           LQFAID+LQASYS VGE DLHT+ADRVADIKNWL++VISYQQSCLDGL EFDPQL+Q+MQ
Sbjct: 121 LQFAIDQLQASYSTVGEPDLHTNADRVADIKNWLTSVISYQQSCLDGLEEFDPQLRQKMQ 180

Query: 181 DGLDVAGKLTSNALAIVTAVSNILDNYRLQLKVQPSGRRLLGTTVVDRDGFPTWLTGADR 240
           DGL+ AGKLTSNALAIV AVS+IL ++ LQLK QPSGRRLLGTT VD DG+PTWLTGADR
Sbjct: 181 DGLNGAGKLTSNALAIVDAVSDILASFGLQLKAQPSGRRLLGTTEVDNDGYPTWLTGADR 240

Query: 241 KLLASKQGGVRPTPNAVVAKDGSGKYKTIAAALAAYPKVLRGRYVIYVKAGIYDEYITLT 300
           KLLAS+ GG +  PNAVVAKDGSG++KTI+AALAAYPK LRGRYVIYVKAGIY EY+ +T
Sbjct: 241 KLLASRGGGAKVKPNAVVAKDGSGQFKTISAALAAYPKTLRGRYVIYVKAGIYKEYVHIT 300

Query: 301 KDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQ 360
           KDMKN+FMYGDGP+KTIVTG KSNR GFTTQ+TA+F AIGEGFLCKSMGF NTAGPEGHQ
Sbjct: 301 KDMKNIFMYGDGPKKTIVTGNKSNRGGFTTQDTATFIAIGEGFLCKSMGFQNTAGPEGHQ 360

Query: 361 AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVVSGTVDFIFGDSSTVIQNSLII 420
           AVALRVQSDRSAFFNCRMDG+QDTLYVQT RQFYRNCV+SGTVDFIFGDS+TVIQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGYQDTLYVQTQRQFYRNCVISGTVDFIFGDSTTVIQNSLII 420

Query: 421 VRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480
           VRRPMDNQQNTVTA GRA+ KE++GLVIHNCRIVPEQKLFAERFKIPTFLGRPWK+YART
Sbjct: 421 VRRPMDNQQNTVTANGRADPKEVSGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYART 480

Query: 481 VIMESTLGDFIQPAGYMPWSGDFALETCLYLEYGNRGPGANTNRRVRWKGAKVIGRNEAL 540
           VIMEST+GDFIQP GYMPWSG+FALETCLYLEYGNRGPGA TNRRVRWKG KVIGRNEA+
Sbjct: 481 VIMESTMGDFIQPVGYMPWSGNFALETCLYLEYGNRGPGAVTNRRVRWKGVKVIGRNEAM 540

Query: 541 QYTAGAFLLGRSWLPTTGGLYYLGLKN 568
           Q+TAG+FL G++WLPTTGG Y LGLK+
Sbjct: 541 QFTAGSFLQGKTWLPTTGGPYLLGLKS 566