CSPI04G26850 (gene) Wild cucumber (PI 183967)

NameCSPI04G26850
Typegene
OrganismCucumis sativus (Wild cucumber (PI 183967))
DescriptionAMP-dependent synthetase and ligase family protein
LocationChr4 : 23788487 .. 23791049 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTTGTTTACTCAATTGTTCTAGAAAAAGTTAGTATCAATCAATCAATATCATGGCGAGACGACGTCGAAGAGACGCCACTATCCTTCTGAGCTAAAGGATCAGCCACTGATTTCTTCTTCTCTATCAATCCCTCCAAAATCCATGGACGGAAGCCACCGTTGCTCTGCTAACTACGTTCCTCTCACTCCAATCTCCTTCTTGGAGCGCTCTGCCGCCGTCTATGGTGACCGGATTTCTCTTGTCTATGGACGCGTTCAGTATACTTGGCGAGATACGCTTCAGCGATGTACCAGGCTCGCTTCTGCTCTTGTTCGTACGGGAATCGCTCGTGGAGATGTGGTATGTATTCAACAACTTTCTATATATTTGTTTGTGTTTTTCTTATTTTACTCGATTTTTTGAGGTCATTGTGTTTATGTGGAACATTGAATTGTGCAAGTTGCTTCGATGGCCTTTTTTTTTATATATATATATTTATGATCATGAGGTTCAATTGCGACGATTTGAAGTTTACGGCTCTAGTTTTGTGTTTATGGTGATAGTTATTCGGATGGATGAACTCTGGTTCCCGTCTTATTATGATCAATTGTGAAATTGAAAGTAGTATTTGGTTAGATTAAGGTCTTTCTGGGTTAAACTTTCTCGATATAATTTTGGAAATGAAATGCGATTCTCTCGAGCAGGTTGCTGCCTTGGTACCGAATATTCCAGCTATGTACGAGTTACATTTTGCTGTGCCAATGGCTGGTGCAGTTCTTTGCTCCCTCAACACACGCCATGATGCAGCAATGGTTTCAACATTGCTAAGCCACTCAGAAGCTAAAATTATTGTTGTAGACTACCAGCTTGAACATATCGTAACCGGAGCAATTAAAGCCATGTCCGAAAGGAAAGAAAAGCTACCTCGTGTCGTTATTATTCAAGAGTATGATCAGCCACCTTCCCGCATCGATAGACCGGGATCTGCTTTAGAATATCTGGAGTTTGAGAGCTTTTTAGCATCTGGAAAACTCAATTTTGAGATTAGGCGACCTAGGGATGAATTGGATCCAATTGCTCTTAACTATACTTCAGGCACAACATCGAGGCCAAAGGGTGTTATTTTCTCCCATAGAGGCGCATATCTCAATTCCCTATCTGCAGTCCTACTGAATGATATGTGTTCACTTCCTGTGTATTTGTGGACTGTTCCAATGTTTCATTGCAATGGATGGTGTTTAACTTGGGGCGTAGCTGCACAGAGCGGCACAAACATCTGCCAGAGAAATGTGACTGCCAAAGAAATCTTTGATAATATTTCTCTGCATAAGGTTACTCATATGGGCGGTGCACCAACAGTTTCGAACATGATTATCAATGCACCAATTAGTGAACAGAAGCCACTTCCCAGGGAAGTAACTATGATGTCTGGTGGCGCTCCGCCGCCTTCTCATGTACTCTATAAGCTTAAAGCTTTGGGATTCCGTATTGTCCATTCATATGGTTTAACTGAAACATATGGGCCGGCAACAGTTTGCTCTTGGAAGCCTGAATGGGATTCTCTACCACAAGATAAACAAGCAAAACTAAATTCTCGCCAAGGATTGCAGCATATTGGGCTAGAGGCAGCAGACGTAAAGGATCCTGTCACAATGGAGAGTGTTCCAGCTGATGGGAAAACCATGGGTGAAGTTATGCTGAGAGGCAATACTGTGATGAGTGGATATTTGCAAGATCTCAAAGCCACACGGGAAGCGTTTAATGGCGGATGGTTTCGAAGTGGGGACTTAGGAGTCAAACACCCTGATGGTTATATAGAATTGAAGGACCGTTCAAAGGACATCATCATTTCTGGGGGAGAAAATATTAGCACAATTGAAGTGGAGTCTGTTCTTTTCAGTCATCCATCAGTTCTTGACGCTGCTGTTGTGGGAAGACCTGATGATCACTGGGGAGAAACACCATGCGCATTTGTGAAGCTCAAAGATGGGTGCAGTGCTACTGAAGGAGAGATCATAAAATTCTGTAGAGAACACCTACCTCATTACATGGCTCCACGAAGTGTTGTATTTAGGGATTTACCTAAAACTTCTACAGGGAAAACTCAAAAATTTATTCTCAAGAAGGAAGCCAAAGCCATGGGTAGCCTTCCAAAACGGGTTAGTAAACTGTAAATACATTCTTGAAAGGGAAGGCAACGGTGTTGGAAAGGCAATGATGTTTGAATAATTAAGGTCCTTATTGTTTGTGTCTTGATTGGTATCAATTTTAGTTCTCCACAGATGTGCACAGGCCTACAAATCTTCTCTGTTTTGATTGCATTTAATGTTGTACTTTTACATTTTCAGGAAAATTAAGAACAAAAAGAGAGTCTGTCTTATTTCTATTTATTGAAGTTGTTGTAATGGTACGATTAATATTGCACTTTTTTTCTTTCGACAAACATTGGTCAAATATGTGAAAGTACATATTCATTATTACTACTTCCGGTTCTGGTACTTAATTTTGATCTTTCACGGAGTTTACTTCTTCTAAATTGTATTGATATTTCTTTATTTATGGTTAAATTTTAATATTACTCT

mRNA sequence

ATGGACGGAAGCCACCGTTGCTCTGCTAACTACGTTCCTCTCACTCCAATCTCCTTCTTGGAGCGCTCTGCCGCCGTCTATGGTGACCGGATTTCTCTTGTCTATGGACGCGTTCAGTATACTTGGCGAGATACGCTTCAGCGATGTACCAGGCTCGCTTCTGCTCTTGTTCGTACGGGAATCGCTCGTGGAGATGTGGTTGCTGCCTTGGTACCGAATATTCCAGCTATGTACGAGTTACATTTTGCTGTGCCAATGGCTGGTGCAGTTCTTTGCTCCCTCAACACACGCCATGATGCAGCAATGGTTTCAACATTGCTAAGCCACTCAGAAGCTAAAATTATTGTTGTAGACTACCAGCTTGAACATATCGTAACCGGAGCAATTAAAGCCATGTCCGAAAGGAAAGAAAAGCTACCTCGTGTCGTTATTATTCAAGAGTATGATCAGCCACCTTCCCGCATCGATAGACCGGGATCTGCTTTAGAATATCTGGAGTTTGAGAGCTTTTTAGCATCTGGAAAACTCAATTTTGAGATTAGGCGACCTAGGGATGAATTGGATCCAATTGCTCTTAACTATACTTCAGGCACAACATCGAGGCCAAAGGGTGTTATTTTCTCCCATAGAGGCGCATATCTCAATTCCCTATCTGCAGTCCTACTGAATGATATGTGTTCACTTCCTGTGTATTTGTGGACTGTTCCAATGTTTCATTGCAATGGATGGTGTTTAACTTGGGGCGTAGCTGCACAGAGCGGCACAAACATCTGCCAGAGAAATGTGACTGCCAAAGAAATCTTTGATAATATTTCTCTGCATAAGGTTACTCATATGGGCGGTGCACCAACAGTTTCGAACATGATTATCAATGCACCAATTAGTGAACAGAAGCCACTTCCCAGGGAAGTAACTATGATGTCTGGTGGCGCTCCGCCGCCTTCTCATGTACTCTATAAGCTTAAAGCTTTGGGATTCCGTATTGTCCATTCATATGGTTTAACTGAAACATATGGGCCGGCAACAGTTTGCTCTTGGAAGCCTGAATGGGATTCTCTACCACAAGATAAACAAGCAAAACTAAATTCTCGCCAAGGATTGCAGCATATTGGGCTAGAGGCAGCAGACGTAAAGGATCCTGTCACAATGGAGAGTGTTCCAGCTGATGGGAAAACCATGGGTGAAGTTATGCTGAGAGGCAATACTGTGATGAGTGGATATTTGCAAGATCTCAAAGCCACACGGGAAGCGTTTAATGGCGGATGGTTTCGAAGTGGGGACTTAGGAGTCAAACACCCTGATGGTTATATAGAATTGAAGGACCGTTCAAAGGACATCATCATTTCTGGGGGAGAAAATATTAGCACAATTGAAGTGGAGTCTGTTCTTTTCAGTCATCCATCAGTTCTTGACGCTGCTGTTGTGGGAAGACCTGATGATCACTGGGGAGAAACACCATGCGCATTTGTGAAGCTCAAAGATGGGTGCAGTGCTACTGAAGGAGAGATCATAAAATTCTGTAGAGAACACCTACCTCATTACATGGCTCCACGAAGTGTTGTATTTAGGGATTTACCTAAAACTTCTACAGGGAAAACTCAAAAATTTATTCTCAAGAAGGAAGCCAAAGCCATGGGTAGCCTTCCAAAACGGGTTAGTAAACTGTAA

Coding sequence (CDS)

ATGGACGGAAGCCACCGTTGCTCTGCTAACTACGTTCCTCTCACTCCAATCTCCTTCTTGGAGCGCTCTGCCGCCGTCTATGGTGACCGGATTTCTCTTGTCTATGGACGCGTTCAGTATACTTGGCGAGATACGCTTCAGCGATGTACCAGGCTCGCTTCTGCTCTTGTTCGTACGGGAATCGCTCGTGGAGATGTGGTTGCTGCCTTGGTACCGAATATTCCAGCTATGTACGAGTTACATTTTGCTGTGCCAATGGCTGGTGCAGTTCTTTGCTCCCTCAACACACGCCATGATGCAGCAATGGTTTCAACATTGCTAAGCCACTCAGAAGCTAAAATTATTGTTGTAGACTACCAGCTTGAACATATCGTAACCGGAGCAATTAAAGCCATGTCCGAAAGGAAAGAAAAGCTACCTCGTGTCGTTATTATTCAAGAGTATGATCAGCCACCTTCCCGCATCGATAGACCGGGATCTGCTTTAGAATATCTGGAGTTTGAGAGCTTTTTAGCATCTGGAAAACTCAATTTTGAGATTAGGCGACCTAGGGATGAATTGGATCCAATTGCTCTTAACTATACTTCAGGCACAACATCGAGGCCAAAGGGTGTTATTTTCTCCCATAGAGGCGCATATCTCAATTCCCTATCTGCAGTCCTACTGAATGATATGTGTTCACTTCCTGTGTATTTGTGGACTGTTCCAATGTTTCATTGCAATGGATGGTGTTTAACTTGGGGCGTAGCTGCACAGAGCGGCACAAACATCTGCCAGAGAAATGTGACTGCCAAAGAAATCTTTGATAATATTTCTCTGCATAAGGTTACTCATATGGGCGGTGCACCAACAGTTTCGAACATGATTATCAATGCACCAATTAGTGAACAGAAGCCACTTCCCAGGGAAGTAACTATGATGTCTGGTGGCGCTCCGCCGCCTTCTCATGTACTCTATAAGCTTAAAGCTTTGGGATTCCGTATTGTCCATTCATATGGTTTAACTGAAACATATGGGCCGGCAACAGTTTGCTCTTGGAAGCCTGAATGGGATTCTCTACCACAAGATAAACAAGCAAAACTAAATTCTCGCCAAGGATTGCAGCATATTGGGCTAGAGGCAGCAGACGTAAAGGATCCTGTCACAATGGAGAGTGTTCCAGCTGATGGGAAAACCATGGGTGAAGTTATGCTGAGAGGCAATACTGTGATGAGTGGATATTTGCAAGATCTCAAAGCCACACGGGAAGCGTTTAATGGCGGATGGTTTCGAAGTGGGGACTTAGGAGTCAAACACCCTGATGGTTATATAGAATTGAAGGACCGTTCAAAGGACATCATCATTTCTGGGGGAGAAAATATTAGCACAATTGAAGTGGAGTCTGTTCTTTTCAGTCATCCATCAGTTCTTGACGCTGCTGTTGTGGGAAGACCTGATGATCACTGGGGAGAAACACCATGCGCATTTGTGAAGCTCAAAGATGGGTGCAGTGCTACTGAAGGAGAGATCATAAAATTCTGTAGAGAACACCTACCTCATTACATGGCTCCACGAAGTGTTGTATTTAGGGATTTACCTAAAACTTCTACAGGGAAAACTCAAAAATTTATTCTCAAGAAGGAAGCCAAAGCCATGGGTAGCCTTCCAAAACGGGTTAGTAAACTGTAA
BLAST of CSPI04G26850 vs. Swiss-Prot
Match: AAE1_ARATH (Probable acyl-activating enzyme 1, peroxisomal OS=Arabidopsis thaliana GN=AAE1 PE=2 SV=1)

HSP 1 Score: 780.8 bits (2015), Expect = 1.0e-224
Identity = 365/551 (66.24%), Postives = 448/551 (81.31%), Query Frame = 1

Query: 1   MDGSHRCSANYVPLTPISFLERSAAVYGDRISLVYGRVQYTWRDTLQRCTRLASALVRTG 60
           M+G+ +  ANYVPLTPISFL+RSA VY DR+S+VYG V+YTWR T  RC R+ASAL + G
Sbjct: 3   MEGTIKSPANYVPLTPISFLDRSAVVYADRVSIVYGSVKYTWRQTRDRCVRIASALSQLG 62

Query: 61  IARGDVVAALVPNIPAMYELHFAVPMAGAVLCSLNTRHDAAMVSTLLSHSEAKIIVVDYQ 120
           I+ GDVV+ L PN+PAM ELHF VPMAGA+LC+LN RHD+++V+ LL HS  K+I  D+Q
Sbjct: 63  ISTGDVVSVLAPNVPAMVELHFGVPMAGALLCTLNIRHDSSLVAVLLRHSGTKVIFADHQ 122

Query: 121 LEHIVTGAIKAMSERKEKLPRVVIIQEYDQPPSRIDRPGSALEYLEFESFLASGKLNFEI 180
              I  GA + +S + +K+P +V+I E       + R   + E +E+E  +A GK +FE+
Sbjct: 123 FLQIAEGACEILSNKGDKVPILVLIPE--PLTQSVSRKKRSEEMMEYEDVVAMGKSDFEV 182

Query: 181 RRPRDELDPIALNYTSGTTSRPKGVIFSHRGAYLNSLSAVLLNDMCSLPVYLWTVPMFHC 240
            RP DE D I++NYTSGTTS PKGV++SHRGAYLNSL+AVLLN+M S P YLWT PMFHC
Sbjct: 183 IRPTDECDAISVNYTSGTTSSPKGVVYSHRGAYLNSLAAVLLNEMHSSPTYLWTNPMFHC 242

Query: 241 NGWCLTWGVAAQSGTNICQRNVTAKEIFDNISLHKVTHMGGAPTVSNMIINAPISEQKPL 300
           NGWCL WGV A  GTNIC RNVTAK IFDNIS HKVTHMGGAPT+ NMIINAP SEQKPL
Sbjct: 243 NGWCLLWGVTAIGGTNICLRNVTAKAIFDNISQHKVTHMGGAPTILNMIINAPESEQKPL 302

Query: 301 PREVTMMSGGAPPPSHVLYKLKALGFRIVHSYGLTETYGPATVCSWKPEWDSLPQDKQAK 360
           P +V+ ++G APPP+HV++K++ LGF + HSYGLTETYGP T+C+WKPEWDSLP+++QAK
Sbjct: 303 PGKVSFITGAAPPPAHVIFKMEELGFSMFHSYGLTETYGPGTICTWKPEWDSLPREEQAK 362

Query: 361 LNSRQGLQHIGLEAADVKDPVTMESVPADGKTMGEVMLRGNTVMSGYLQDLKATREAFNG 420
           + +RQG+ H+GLE   VKDPVTM ++PADG TMGEV+ RGNTVM+GYL++ +AT+EAF G
Sbjct: 363 MKARQGVNHLGLEEIQVKDPVTMRTLPADGVTMGEVVFRGNTVMNGYLKNPEATKEAFKG 422

Query: 421 GWFRSGDLGVKHPDGYIELKDRSKDIIISGGENISTIEVESVLFSHPSVLDAAVVGRPDD 480
           GWF SGDLGVKHPDGYIELKDRSKDIIISGGENIS+IEVES LF+HP VL+AAVV RPD+
Sbjct: 423 GWFWSGDLGVKHPDGYIELKDRSKDIIISGGENISSIEVESTLFTHPCVLEAAVVARPDE 482

Query: 481 HWGETPCAFVKLKDGCSATEGEIIKFCREHLPHYMAPRSVVFRDLPKTSTGKTQKFILKK 540
           +WGET CAFVKLKDG  A+  E+I +CR+ LPHYMAPRS+VF DLPKTSTGK QKF+L+ 
Sbjct: 483 YWGETACAFVKLKDGSKASAEELISYCRDRLPHYMAPRSIVFEDLPKTSTGKVQKFVLRT 542

Query: 541 EAKAMGSLPKR 552
           +AKA+ SL K+
Sbjct: 543 KAKALVSLSKK 551

BLAST of CSPI04G26850 vs. Swiss-Prot
Match: AAE2_ARATH (Probable acyl-activating enzyme 2 OS=Arabidopsis thaliana GN=AAE2 PE=2 SV=1)

HSP 1 Score: 726.9 bits (1875), Expect = 1.7e-208
Identity = 352/556 (63.31%), Postives = 431/556 (77.52%), Query Frame = 1

Query: 1   MDGSHRCSANYVPLTPISFLERSAAVYGDRISLVYGRVQYTWRDTLQRCTRLASALVRTG 60
           ++G  R  AN+ PL+PI+FLERSA VY DR SLV+G V++TW  T QRC RLASAL   G
Sbjct: 48  IEGLLRSPANFSPLSPITFLERSAKVYRDRTSLVFGSVKHTWFQTYQRCLRLASALTNLG 107

Query: 61  IARGDVVAALVPNIPAMYELHFAVPMAGAVLCSLNTRHDAAMVSTLLSHSEAKIIVVDYQ 120
           I+RGDVVAAL PN+PAM+ELHFAVPMAG +LC LNTR D + +S LL+HSEAKI+ VD+Q
Sbjct: 108 ISRGDVVAALAPNVPAMHELHFAVPMAGLILCPLNTRLDPSTLSVLLAHSEAKILFVDHQ 167

Query: 121 LEHIVTGAIKAMSER---KEKLPRVVIIQEYDQPPSRIDRPGS-ALEY---LEFESFLAS 180
           L  I  GA+  +++    ++ L  V+I Q  D   S  D   + A +Y    E+E+ L S
Sbjct: 168 LLEIAHGALDLLAKSDKTRKSLKLVLISQSNDDDDSDEDSSSTFASKYSFDYEYETLLKS 227

Query: 181 GKLNFEIRRPRDELDPIALNYTSGTTSRPKGVIFSHRGAYLNSLSAVLLNDMCSLPVYLW 240
           G   FEI +PR E DPI++NYTSGTTSRPKGV++SHRGAYLNSL+ V L+ M   PVYLW
Sbjct: 228 GDSEFEIIKPRCEWDPISINYTSGTTSRPKGVVYSHRGAYLNSLATVFLHQMSVYPVYLW 287

Query: 241 TVPMFHCNGWCLTWGVAAQSGTNICQRNVTAKEIFDNISLHKVTHMGGAPTVSNMIINAP 300
           TVPMFHCNGWCL WGVAAQ GTNIC R V+ K IF NI++HKVTHMGGAPTV NMI+N  
Sbjct: 288 TVPMFHCNGWCLVWGVAAQGGTNICLRKVSPKMIFKNIAMHKVTHMGGAPTVLNMIVNYT 347

Query: 301 ISEQKPLPREVTMMSGGAPPPSHVLYKLKALGFRIVHSYGLTETYGPATVCSWKPEWDSL 360
           ++E KPLP  V +M+GG+PP   +L K++ LGF + H YGLTETYGP T C WKPEWDSL
Sbjct: 348 VTEHKPLPHRVEIMTGGSPPLPQILAKMEELGFNVSHLYGLTETYGPGTHCVWKPEWDSL 407

Query: 361 PQDKQAKLNSRQGLQHIGLEAADVKDPVTMESVPADGKTMGEVMLRGNTVMSGYLQDLKA 420
             +++ KL +RQG+QH+GLE  DVKDP+TME+VP DG TMGEVM RGNTVMSGY +D++A
Sbjct: 408 SLEERTKLKARQGVQHLGLEGLDVKDPLTMETVPDDGLTMGEVMFRGNTVMSGYFKDIEA 467

Query: 421 TREAFNGGWFRSGDLGVKHPDGYIELKDRSKDIIISGGENISTIEVESVLFSHPSVLDAA 480
           TR+AF G WF SGDL VK+PDGYIE+KDR KD+IISGGENIS++EVE VL SH +VL+AA
Sbjct: 468 TRKAFEGDWFHSGDLAVKYPDGYIEIKDRLKDVIISGGENISSVEVERVLCSHQAVLEAA 527

Query: 481 VVGRPDDHWGETPCAFVKLKDGCSATE-GEIIKFCREHLPHYMAPRSVVFRDLPKTSTGK 540
           VV RPD HWG+TPC FVKLK+G    +  EII FCR+HLPHYMAP+++VF D+PKTSTGK
Sbjct: 528 VVARPDHHWGQTPCGFVKLKEGFDTIKPEEIIGFCRDHLPHYMAPKTIVFGDIPKTSTGK 587

Query: 541 TQKFILKKEAKAMGSL 549
            QK++L+K+A  MGSL
Sbjct: 588 VQKYLLRKKADEMGSL 603

BLAST of CSPI04G26850 vs. Swiss-Prot
Match: AEE7_ARATH (Acetate/butyrate--CoA ligase AAE7, peroxisomal OS=Arabidopsis thaliana GN=AAE7 PE=1 SV=1)

HSP 1 Score: 596.3 bits (1536), Expect = 3.5e-169
Identity = 293/559 (52.42%), Postives = 387/559 (69.23%), Query Frame = 1

Query: 1   MDGSHRCSANYVPLTPISFLERSAAVYGDRISLVYGRVQYTWRDTLQRCTRLASALVRTG 60
           +D   +  ANY  LTP+ FL+R+A V+  R S+++G  +YTWR T  RC RLASAL    
Sbjct: 9   IDDLPKIPANYTALTPLWFLDRAAVVHPTRKSVIHGSREYTWRQTYDRCRRLASALADRS 68

Query: 61  IARGDVVAALVPNIPAMYELHFAVPMAGAVLCSLNTRHDAAMVSTLLSHSEAKIIVVDYQ 120
           I  G  VA + PNIPAMYE HF VPM GAVL  +N R +A  V+ LLSHS++ +I+VD +
Sbjct: 69  IGPGSTVAIIAPNIPAMYEAHFGVPMCGAVLNCVNIRLNAPTVAFLLSHSQSSVIMVDQE 128

Query: 121 LEHIVTGAIKAMSERKE---KLPRVVIIQEYDQPPSRIDRPGSALEYLEFESFLASGKLN 180
              +   +++ M E+     K P +++I ++   P  ++R  S    +E+E FLA+G  N
Sbjct: 129 FFTLAEDSLRLMEEKAGSSFKRPLLIVIGDHTCAPESLNRALSK-GAIEYEDFLATGDPN 188

Query: 181 FEIRRPRDELDPIALNYTSGTTSRPKGVIFSHRGAYLNSLSAVLLNDMCSLPVYLWTVPM 240
           +  + P DE   IAL YTSGTT+ PKGV+  HRGAY+ +LS  L+  M    VYLWT+PM
Sbjct: 189 YPWQPPADEWQSIALGYTSGTTASPKGVVLHHRGAYIMALSNPLIWGMQDGAVYLWTLPM 248

Query: 241 FHCNGWCLTWGVAAQSGTNICQRNVTAKEIFDNISLHKVTHMGGAPTVSNMIINAPISEQ 300
           FHCNGWC  W +A  SGT+IC R VTAKE++  I+ +KVTH   AP V N I+NAP  + 
Sbjct: 249 FHCNGWCFPWSLAVLSGTSICLRQVTAKEVYSMIAKYKVTHFCAAPVVLNAIVNAPKEDT 308

Query: 301 -KPLPREVTMMSGGAPPPSHVLYKLKALGFRIVHSYGLTETYGPATVCSWKPEWDSLPQD 360
             PLP  V +M+ GA PP  VL+ +   GFR+ H+YGL+ETYGP+TVC+WKPEWDSLP +
Sbjct: 309 ILPLPHTVHVMTAGAAPPPSVLFSMNQKGFRVAHTYGLSETYGPSTVCAWKPEWDSLPPE 368

Query: 361 KQAKLNSRQGLQHIGLEAADVKDPVTMESVPADGKTMGEVMLRGNTVMSGYLQDLKATRE 420
            QAKLN+RQG+++ G+E  DV D  T + VPADGKT GE++ RGN VM GYL++ +A +E
Sbjct: 369 TQAKLNARQGVRYTGMEQLDVIDTQTGKPVPADGKTAGEIVFRGNMVMKGYLKNPEANKE 428

Query: 421 AFNGGWFRSGDLGVKHPDGYIELKDRSKDIIISGGENISTIEVESVLFSHPSVLDAAVVG 480
            F GGWF SGD+ VKHPD YIE+KDRSKD+IISGGENIS++EVE+V++ HP+VL+A+VV 
Sbjct: 429 TFAGGWFHSGDIAVKHPDNYIEIKDRSKDVIISGGENISSVEVENVVYHHPAVLEASVVA 488

Query: 481 RPDDHWGETPCAFVKLKDGCSATE-----GEIIKFCREHLPHYMAPRSVVFRDLPKTSTG 540
           RPD+ W E+PCAFV LK      +      +I+KFCRE LP Y  P+SVVF  LPKT+TG
Sbjct: 489 RPDERWQESPCAFVTLKSDYEKHDQNKLAQDIMKFCREKLPAYWVPKSVVFGPLPKTATG 548

Query: 541 KTQKFILKKEAKAMGSLPK 551
           K QK IL+ +AK MG +P+
Sbjct: 549 KIQKHILRTKAKEMGPVPR 566

BLAST of CSPI04G26850 vs. Swiss-Prot
Match: AEE21_ARATH (Probable acyl-activating enzyme 21 OS=Arabidopsis thaliana GN=AEE21 PE=3 SV=1)

HSP 1 Score: 586.6 bits (1511), Expect = 2.8e-166
Identity = 290/554 (52.35%), Postives = 383/554 (69.13%), Query Frame = 1

Query: 1   MDGSHRCSANYVPLTPISFLERSAAVYGDRISLVYGRVQYTWRDTLQRCTRLASALVRTG 60
           M+G+ RCSANYVPL+PISFLER+A V+G R S+VYG +QYTW  T  RC RLASAL   G
Sbjct: 1   MEGTMRCSANYVPLSPISFLERAAVVFGSRTSVVYGDIQYTWHQTRDRCVRLASALSDLG 60

Query: 61  IARGDVVAALVPNIPAMYELHFAVPMAGAVLCSLNTRHDAAMVSTLLSHSEAKIIVVDYQ 120
           ++R DVVAAL PN+PA+ EL+F  PMAGAVLC LNT  D+ M++  L  ++ K+  VD +
Sbjct: 61  LSRHDVVAALAPNVPALCELYFGAPMAGAVLCVLNTTFDSQMLAMALEKTKPKVFFVDSE 120

Query: 121 LEHIVTGAIKAMSERKEKLPRVVIIQEYDQPPSRIDRPGSALEYLEFESFLASGKLNFEI 180
              +   ++  +S  +EK P ++ I E          P    +Y ++E FL++G  NF+ 
Sbjct: 121 FLSVAEESLSLLSNIEEK-PLIITITE---------NPTEQSKYEQYEDFLSTGNPNFKP 180

Query: 181 RRPRDELDPIALNYTSGTTSRPKGVIFSHRGAYLNSLSAVLLNDMCSLPVYLWTVPMFHC 240
            RP DE DPIALN+TSGTTS PK V++SHRGAYLN+ +  ++N+M  +PVYL TVPM+HC
Sbjct: 181 IRPVDECDPIALNFTSGTTSTPKCVVYSHRGAYLNATAVGVMNEMKPMPVYLCTVPMYHC 240

Query: 241 NGWCLTWGVAAQSGTNICQRNVTAKEIFDNISLHKVTHMGGAPTVSNMIINAPISEQKPL 300
           +GWC  W V A  G  +C R V  + IFD+I  HKVT+ GG+P V NMI NA  S +K  
Sbjct: 241 SGWCYIWTVTAFGGVIVCLREVNDEVIFDSIVKHKVTNFGGSPPVLNMIANARDSVKKSF 300

Query: 301 PREVTMMSGGAPPPSHVLYKLKALGFRIVHSYGLTETYGPATVCSWKPEWDSLPQDKQAK 360
           P  V +MSGG+ PP  V+ KLK LGF+++ +YG +E YG  T C W PEW++LP+++  +
Sbjct: 301 PWTVQVMSGGSSPPE-VMLKLKKLGFKVMMAYGCSEVYGLGTACLWMPEWETLPEEESLR 360

Query: 361 LNSRQGLQHIGLEAADVKDPVTMESVPADGKTMGEVMLRGNTVMSGYLQDLKATREAFNG 420
           L +R GL H   EA DV DP TM+SVP DGKT+  + LRGNTVMSGY +D +AT  AF G
Sbjct: 361 LKARDGLNHFAKEAVDVLDPTTMKSVPHDGKTIRVIALRGNTVMSGYFKDKEATEAAFRG 420

Query: 421 GWFRSGDLGVKHPDGYIELKDRSKDIIISGGENISTIEVESVLFSHPSVLDAAVVGRPDD 480
           GW+ S D+GV  PDGYI+ KDRS+D+I  GGE + + E+E +L+SHP+V DA VVGRPD+
Sbjct: 421 GWYWSRDMGVIDPDGYIQFKDRSQDVITCGGEIVGSKEIEGILYSHPAVYDAGVVGRPDE 480

Query: 481 HWGETPCAFVKLKDGCSATEGEIIKFCREHLPH---YMAPRSVVFRDLPKTSTGKTQKFI 540
             GE+ CAFVKLK+G  A E EII+FC+  L +    M P++VVF D+PKT TGK +K +
Sbjct: 481 TLGESMCAFVKLKEGAEAREEEIIEFCKRKLGNKNMKMIPKTVVFSDVPKTPTGKIRKNV 540

Query: 541 LKKEAKAMGSLPKR 552
           L+K AK MG +  R
Sbjct: 541 LRKMAKDMGYVQLR 543

BLAST of CSPI04G26850 vs. Swiss-Prot
Match: AAE11_ARATH (Butyrate--CoA ligase AAE11, peroxisomal OS=Arabidopsis thaliana GN=AAE11 PE=1 SV=1)

HSP 1 Score: 569.7 bits (1467), Expect = 3.5e-161
Identity = 272/554 (49.10%), Postives = 386/554 (69.68%), Query Frame = 1

Query: 1   MDGSHRCSANYVPLTPISFLERSAAVYGDRISLVYGRVQYTWRDTLQRCTRLASALVRTG 60
           MD    C AN VPLTPI+FL+R++  Y +R S++YG+ ++TW  T  RC RLA++L+   
Sbjct: 1   MDNLVLCEANNVPLTPITFLKRASECYPNRTSIIYGQTRFTWPQTYDRCCRLAASLLSLN 60

Query: 61  IARGDVVAALVPNIPAMYELHFAVPMAGAVLCSLNTRHDAAMVSTLLSHSEAKIIVVDYQ 120
           I R DVV+ L PN+PAMYE+HF+VPM GAVL  +NTR DA  ++ +L H+E KI+ VDY+
Sbjct: 61  ITRNDVVSILAPNVPAMYEMHFSVPMTGAVLNPINTRLDAKTIAIILRHAEPKILFVDYE 120

Query: 121 LEHIVTGAIKAM-SERKEKLPRVVIIQEYDQPPSRIDRPGSALEYLEFESFLASGK---- 180
              ++   ++ + + + +  PR+++I E D       +       L++E  +  G+    
Sbjct: 121 FAPLIQEVLRLIPTYQSQPHPRIILINEIDSTTKPFSKE------LDYEGLIRKGEPTPS 180

Query: 181 LNFEIRRPRDELDPIALNYTSGTTSRPKGVIFSHRGAYLNSLSAVLLNDMCSLPVYLWTV 240
            +  + R  +E DPI+LNYTSGTT+ PKGV+ SH+GAYL++LS+++  +M   PVYLWT+
Sbjct: 181 SSASMFRVHNEHDPISLNYTSGTTADPKGVVISHQGAYLSALSSIIGWEMGIFPVYLWTL 240

Query: 241 PMFHCNGWCLTWGVAAQSGTNICQRNVTAKEIFDNISLHKVTHMGGAPTVSNMIINAPIS 300
           PMFHCNGW  TW VAA+ GTN+C R+VTA EI+ NI LH VTHM   PTV   ++    +
Sbjct: 241 PMFHCNGWTHTWSVAARGGTNVCIRHVTAPEIYKNIELHGVTHMSCVPTVFRFLLEGSRT 300

Query: 301 EQKPLPREVTMMSGGAPPPSHVLYKLKALGFRIVHSYGLTETYGPATVCSWKPEWDSLPQ 360
           +Q P    V +++GG+ PP+ ++ K++ LGF ++H YGLTE  GP   C W+ EW+ LP+
Sbjct: 301 DQSPKSSPVQVLTGGSSPPAVLIKKVEQLGFHVMHGYGLTEATGPVLFCEWQDEWNKLPE 360

Query: 361 DKQAKLNSRQGLQHIGLEAADVKDPVTMESVPADGKTMGEVMLRGNTVMSGYLQDLKATR 420
            +Q +L  RQG++++ L   DVK+  T+ESVP DGKTMGE++++G+++M GYL++ KAT 
Sbjct: 361 HQQIELQQRQGVRNLTLADVDVKNTKTLESVPRDGKTMGEIVIKGSSLMKGYLKNPKATS 420

Query: 421 EAFNGGWFRSGDLGVKHPDGYIELKDRSKDIIISGGENISTIEVESVLFSHPSVLDAAVV 480
           EAF  GW  +GD+GV HPDGY+E+KDRSKDIIISGGENIS+IEVE VL+ +  VL+AAVV
Sbjct: 421 EAFKHGWLNTGDIGVIHPDGYVEIKDRSKDIIISGGENISSIEVEKVLYMYQEVLEAAVV 480

Query: 481 GRPDDHWGETPCAFVKLK---DGCSATEGEIIKFCREHLPHYMAPRSVV-FRDLPKTSTG 540
             P   WGETPCAFV LK   +G   +EG++IK+CRE++PH+M P+ VV F++LPK S G
Sbjct: 481 AMPHPLWGETPCAFVVLKKGEEGLVTSEGDLIKYCRENMPHFMCPKKVVFFQELPKNSNG 540

Query: 541 KTQKFILKKEAKAM 546
           K  K  L+  AKA+
Sbjct: 541 KILKSKLRDIAKAL 548

BLAST of CSPI04G26850 vs. TrEMBL
Match: A0A0A0L1B6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G658530 PE=4 SV=1)

HSP 1 Score: 1129.4 bits (2920), Expect = 0.0e+00
Identity = 554/555 (99.82%), Postives = 555/555 (100.00%), Query Frame = 1

Query: 1   MDGSHRCSANYVPLTPISFLERSAAVYGDRISLVYGRVQYTWRDTLQRCTRLASALVRTG 60
           MDGSHRCSANYVPLTPISFLERSAAVYGDRISLVYGRVQYTWRDTLQRCTRLASALVRTG
Sbjct: 1   MDGSHRCSANYVPLTPISFLERSAAVYGDRISLVYGRVQYTWRDTLQRCTRLASALVRTG 60

Query: 61  IARGDVVAALVPNIPAMYELHFAVPMAGAVLCSLNTRHDAAMVSTLLSHSEAKIIVVDYQ 120
           IARGDVVAALVPNIPAMYELHFAVPMAGAVLCSLNTRHDAAMVSTLLSHSEAKIIVVDYQ
Sbjct: 61  IARGDVVAALVPNIPAMYELHFAVPMAGAVLCSLNTRHDAAMVSTLLSHSEAKIIVVDYQ 120

Query: 121 LEHIVTGAIKAMSERKEKLPRVVIIQEYDQPPSRIDRPGSALEYLEFESFLASGKLNFEI 180
           LEHIVTGAIKAMSERKEKLPRVVIIQEYDQPPSRIDRPGSALEYLEFESFLASGKLNFEI
Sbjct: 121 LEHIVTGAIKAMSERKEKLPRVVIIQEYDQPPSRIDRPGSALEYLEFESFLASGKLNFEI 180

Query: 181 RRPRDELDPIALNYTSGTTSRPKGVIFSHRGAYLNSLSAVLLNDMCSLPVYLWTVPMFHC 240
           RRPRDELDPIALNYTSGTTSRPKGVIFSHRGAYLNSLSAVLLNDMCSLPVYLWTVPMFHC
Sbjct: 181 RRPRDELDPIALNYTSGTTSRPKGVIFSHRGAYLNSLSAVLLNDMCSLPVYLWTVPMFHC 240

Query: 241 NGWCLTWGVAAQSGTNICQRNVTAKEIFDNISLHKVTHMGGAPTVSNMIINAPISEQKPL 300
           NGWCLTWGVAAQSGTNICQRNVTAKEIFDNISLHKVTHMGGAPTVSNMIINAPISEQKPL
Sbjct: 241 NGWCLTWGVAAQSGTNICQRNVTAKEIFDNISLHKVTHMGGAPTVSNMIINAPISEQKPL 300

Query: 301 PREVTMMSGGAPPPSHVLYKLKALGFRIVHSYGLTETYGPATVCSWKPEWDSLPQDKQAK 360
           PREVTMMSGGAPPPSHVLYKLKALGFRIVHSYGLTETYGPATVCSWKPEWDSLPQDKQAK
Sbjct: 301 PREVTMMSGGAPPPSHVLYKLKALGFRIVHSYGLTETYGPATVCSWKPEWDSLPQDKQAK 360

Query: 361 LNSRQGLQHIGLEAADVKDPVTMESVPADGKTMGEVMLRGNTVMSGYLQDLKATREAFNG 420
           LNSRQGLQHIGLEAADVKDPVTMESVPADGKTMGEVMLRGNTVMSGYL+DLKATREAFNG
Sbjct: 361 LNSRQGLQHIGLEAADVKDPVTMESVPADGKTMGEVMLRGNTVMSGYLKDLKATREAFNG 420

Query: 421 GWFRSGDLGVKHPDGYIELKDRSKDIIISGGENISTIEVESVLFSHPSVLDAAVVGRPDD 480
           GWFRSGDLGVKHPDGYIELKDRSKDIIISGGENISTIEVESVLFSHPSVLDAAVVGRPDD
Sbjct: 421 GWFRSGDLGVKHPDGYIELKDRSKDIIISGGENISTIEVESVLFSHPSVLDAAVVGRPDD 480

Query: 481 HWGETPCAFVKLKDGCSATEGEIIKFCREHLPHYMAPRSVVFRDLPKTSTGKTQKFILKK 540
           HWGETPCAFVKLKDGCSATEGEIIKFCREHLPHYMAPRSVVFRDLPKTSTGKTQKFILKK
Sbjct: 481 HWGETPCAFVKLKDGCSATEGEIIKFCREHLPHYMAPRSVVFRDLPKTSTGKTQKFILKK 540

Query: 541 EAKAMGSLPKRVSKL 556
           EAKAMGSLPKRVSKL
Sbjct: 541 EAKAMGSLPKRVSKL 555

BLAST of CSPI04G26850 vs. TrEMBL
Match: A0A0A0L3B4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G658540 PE=4 SV=1)

HSP 1 Score: 880.9 bits (2275), Expect = 7.9e-253
Identity = 424/553 (76.67%), Postives = 485/553 (87.70%), Query Frame = 1

Query: 6   RCSANYVPLTPISFLERSAAVYGDRISLVYGRVQYTWRDTLQRCTRLASALVRTGIARGD 65
           RCSAN VPLTPI+FLER+AAV+GDRISLVYG V++TWR+TLQRCT+ ASALV  GI+RGD
Sbjct: 5   RCSANSVPLTPITFLERAAAVFGDRISLVYGNVRFTWRETLQRCTKFASALVHVGISRGD 64

Query: 66  VVAALVPNIPAMYELHFAVPMAGAVLCSLNTRHDAAMVSTLLSHSEAKIIVVDYQLEHIV 125
           VVA L PN+PA YELHFAVPMAGA+LC+LN RHDAAMVSTLL HSEAKIIV D+Q  HIV
Sbjct: 65  VVAVLAPNVPATYELHFAVPMAGAILCTLNMRHDAAMVSTLLGHSEAKIIVADHQYLHIV 124

Query: 126 TGAIKAMSERK--EKLPRVVIIQEYDQPPSRIDRPGSALEYLEFESFLASGKLNFEIRRP 185
             AI+ MS+    E+LPR+VI+QE+D P S I+   SA + LE+ES L +G L+FEIRRP
Sbjct: 125 KAAIEIMSKTMAAEELPRIVIVQEFDHPSSNINGFDSASDDLEYESLLNTGTLDFEIRRP 184

Query: 186 RDELDPIALNYTSGTTSRPKGVIFSHRGAYLNSLSAVLLNDMCSLPVYLWTVPMFHCNGW 245
            DE DPI+LNYTSGTTSRPKGVI+SHRG YLN+LS VLLNDM S+ VYLWTVPMFHCNGW
Sbjct: 185 IDERDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTVLLNDMSSMAVYLWTVPMFHCNGW 244

Query: 246 CLTWGVAAQSGTNICQRNVTAKEIFDNISLHKVTHMGGAPTVSNMIINAPISEQKPLPRE 305
            +TWGVAAQ GTNICQRNV AKEIF NISLH VTHMGGAPTV NMI+NAPI+EQKPL  +
Sbjct: 245 SMTWGVAAQGGTNICQRNVNAKEIFANISLHNVTHMGGAPTVLNMIVNAPITEQKPLLGK 304

Query: 306 VTMMSGGAPPPSHVLYKLKALGFRIVHSYGLTETYGPATVCSWKPEWDSLPQDKQAKLNS 365
           VT+M+GGAPPPS++LYK++ LGF IVH YGLTETYG AT C WKPEWDSLP +KQAKL S
Sbjct: 305 VTVMTGGAPPPSNILYKMRELGFLIVHCYGLTETYGVATFCHWKPEWDSLPDEKQAKLKS 364

Query: 366 RQGLQHIGLEAADVKDPVTMESVPADGKTMGEVMLRGNTVMSGYLQDLKATREAFNGGWF 425
           RQG+QHIG+E  D+K+P+TMESVPADGKTMGEVM+RGNTVM GYL+D+KAT EAFNGGWF
Sbjct: 365 RQGMQHIGVEGVDIKNPITMESVPADGKTMGEVMIRGNTVMIGYLKDVKATEEAFNGGWF 424

Query: 426 RSGDLGVKHPDGYIELKDRSKDIIISGGENISTIEVESVLFSHPSVLDAAVVGRPDDHWG 485
           RSGDLGV+HPDGYIELKDRSKDIIISGGENISTIEVESVLF+HPSVL+AA+VGRPDDHWG
Sbjct: 425 RSGDLGVRHPDGYIELKDRSKDIIISGGENISTIEVESVLFNHPSVLEAAIVGRPDDHWG 484

Query: 486 ETPCAFVKLKDGCSATEGEIIKFCREHLPHYMAPRSVVFR-DLPKTSTGKTQKFILKKEA 545
           ETPCAFV LK G +ATE EIIKFCR++LPHYMAP++VVF+ DLPK+STGK QK ILK+EA
Sbjct: 485 ETPCAFVNLKHGSNATEEEIIKFCRDNLPHYMAPKTVVFKSDLPKSSTGKIQKLILKEEA 544

Query: 546 KAMGSLPKRVSKL 556
           KAMG+L    ++L
Sbjct: 545 KAMGTLSLAANRL 557

BLAST of CSPI04G26850 vs. TrEMBL
Match: F6I6S6_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_18s0122g01440 PE=4 SV=1)

HSP 1 Score: 874.8 bits (2259), Expect = 5.7e-251
Identity = 412/554 (74.37%), Postives = 481/554 (86.82%), Query Frame = 1

Query: 1   MDGSHRCSANYVPLTPISFLERSAAVYGDRISLVYGRVQYTWRDTLQRCTRLASALVRTG 60
           M+G  RCSAN+VPL+PISFLERSA+VY DR+++VYGRV++TWRDTL+RCTRLASA+   G
Sbjct: 10  MEGMIRCSANHVPLSPISFLERSASVYRDRVAIVYGRVKFTWRDTLERCTRLASAITHLG 69

Query: 61  IARGDVVAALVPNIPAMYELHFAVPMAGAVLCSLNTRHDAAMVSTLLSHSEAKIIVVDYQ 120
           I+RGDVVAAL PNIPAMYELHF VPMAGAVLC+LN RHD+AMVSTLL HSEAK+I VDYQ
Sbjct: 70  ISRGDVVAALAPNIPAMYELHFGVPMAGAVLCTLNIRHDSAMVSTLLKHSEAKMIFVDYQ 129

Query: 121 LEHIVTGAIKAMSERKEKLPRVVIIQEYDQPPSRIDRPGSALEY-LEFESFLASGKLNFE 180
           L     GA++ +S+ + KLPR+V+I E D+    I   G  +   LE+E+ +A GK +F+
Sbjct: 130 LLDTAQGALEILSKTRTKLPRLVLIPECDKLQPTISNSGPTVAGDLEYETLIAMGKTDFD 189

Query: 181 IRRPRDELDPIALNYTSGTTSRPKGVIFSHRGAYLNSLSAVLLNDMCSLPVYLWTVPMFH 240
           IR P DE DPIALNYTSGTTSRPKGVI+SHRGA+LNSL+AVLLN+M S+PVYLWTVPMFH
Sbjct: 190 IRWPNDEWDPIALNYTSGTTSRPKGVIYSHRGAHLNSLAAVLLNEMGSMPVYLWTVPMFH 249

Query: 241 CNGWCLTWGVAAQSGTNICQRNVTAKEIFDNISLHKVTHMGGAPTVSNMIINAPISEQKP 300
           CNGWCLTWGVAAQ GTN+C RNVTAK IFD+IS H+VTHMGGAPTV NMIINAP+ +Q+P
Sbjct: 250 CNGWCLTWGVAAQGGTNVCLRNVTAKGIFDSISQHRVTHMGGAPTVLNMIINAPVGDQRP 309

Query: 301 LPREVTMMSGGAPPPSHVLYKLKALGFRIVHSYGLTETYGPATVCSWKPEWDSLPQDKQA 360
           LP +V +M+GGAPPP  VL+ +K LGF + HSYGLTETYGP TVC+WKPEWD LP DKQA
Sbjct: 310 LPGKVAVMTGGAPPPPQVLFNMKELGFGVFHSYGLTETYGPGTVCTWKPEWDLLPPDKQA 369

Query: 361 KLNSRQGLQHIGLEAADVKDPVTMESVPADGKTMGEVMLRGNTVMSGYLQDLKATREAFN 420
           K+N+RQGL H+G+E  D+KDPVTM+SVP D KT+GEVM RGNTVM+GYL+D+KAT E+F 
Sbjct: 370 KINARQGLHHLGMEEVDIKDPVTMKSVPPDAKTIGEVMFRGNTVMNGYLKDIKATEESFK 429

Query: 421 GGWFRSGDLGVKHPDGYIELKDRSKDIIISGGENISTIEVESVLFSHPSVLDAAVVGRPD 480
           GGWFRSGDLGVKHPDGYIELKDRSKDIIISGGENISTIEVE+VLFSHP++L+AAVVGRPD
Sbjct: 430 GGWFRSGDLGVKHPDGYIELKDRSKDIIISGGENISTIEVEAVLFSHPAILEAAVVGRPD 489

Query: 481 DHWGETPCAFVKLKDGCSATEGEIIKFCREHLPHYMAPRSVVFRDLPKTSTGKTQKFILK 540
           DHWGETPCAFVKLK+GC+A   EIIKFCR  LPHYMAP++VVF DLPKTSTGKTQK+ILK
Sbjct: 490 DHWGETPCAFVKLKEGCNANANEIIKFCRNKLPHYMAPKTVVFYDLPKTSTGKTQKYILK 549

Query: 541 KEAKAMGSLPKRVS 554
           ++AKAMGSL KR S
Sbjct: 550 EKAKAMGSLSKRSS 563

BLAST of CSPI04G26850 vs. TrEMBL
Match: M5VIW5_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa003716mg PE=4 SV=1)

HSP 1 Score: 860.5 bits (2222), Expect = 1.1e-246
Identity = 411/556 (73.92%), Postives = 474/556 (85.25%), Query Frame = 1

Query: 1   MDGSHRCSANYVPLTPISFLERSAAVYGDRISLVYGRVQYTWRDTLQRCTRLASALVRTG 60
           M+GS RCSANY+PL+PISFLERSA VY DR S+VYG + YTWR+TL+RCTRLASAL + G
Sbjct: 1   MEGSIRCSANYIPLSPISFLERSAIVYRDRPSVVYGSIIYTWRETLERCTRLASALSQLG 60

Query: 61  IARGDVVAALVPNIPAMYELHFAVPMAGAVLCSLNTRHDAAMVSTLLSHSEAKIIVVDYQ 120
           I+RGDVVAAL PNIPAMYELHF VPMAGAVLC+LN RHD+ MVS LL HS+AK I VDYQ
Sbjct: 61  ISRGDVVAALAPNIPAMYELHFGVPMAGAVLCTLNVRHDSQMVSVLLKHSDAKFIFVDYQ 120

Query: 121 LEHIVTGAIKAMSERKEKLPRVVIIQEYDQPPSRIDRPGSALEYLEFESFLASGKLNFEI 180
           L H+  GA   +S+ + KLP +V+I E DQ    +  P S    LE+ES L  GKL+FEI
Sbjct: 121 LFHVAKGAFDILSKTRTKLPVLVLIPESDQSSPELCNPISG--NLEYESLLGRGKLDFEI 180

Query: 181 RRPRDELDPIALNYTSGTTSRPKGVIFSHRGAYLNSLSAVLLNDMCSLPVYLWTVPMFHC 240
           RRP+DE DPI+LNYTSGTTS PKGVI+SHRGAYLNS +AVLLN+M S+PVYLW VPMFHC
Sbjct: 181 RRPKDEWDPISLNYTSGTTSSPKGVIYSHRGAYLNSFAAVLLNEMPSMPVYLWCVPMFHC 240

Query: 241 NGWCLTWGVAAQSGTNICQRNVTAKEIFDNISLHKVTHMGGAPTVSNMIINAPISEQKPL 300
           NGWCLTW VAAQ GTNICQRNVTAK IF  IS HKVTHMGGAPTV NMI+NAP +E++PL
Sbjct: 241 NGWCLTWAVAAQGGTNICQRNVTAKGIFSCISQHKVTHMGGAPTVLNMIVNAPENERRPL 300

Query: 301 PREVTMMSGGAPPPSHVLYKLKALGFRIVHSYGLTETYGPATVCSWKPEWDSLPQDKQAK 360
           P +V +M+GGAPPP+ VL+K++ LGF + HSYGLTETYGP TVCSWKPEWDSLP+ +QAK
Sbjct: 301 PGKVIVMTGGAPPPAQVLFKMEELGFNVTHSYGLTETYGPGTVCSWKPEWDSLPRVEQAK 360

Query: 361 LNSRQGLQHIGLEAADVKDPVTMESVPADGKTMGEVMLRGNTVMSGYLQDLKATREAFNG 420
           + SRQGLQH+G+E  DVKDPVTM+SVP+D KTMGEVM RGNTVM+GYL+D +ATR+AF G
Sbjct: 361 IKSRQGLQHLGMEELDVKDPVTMKSVPSDAKTMGEVMFRGNTVMNGYLKDHQATRDAFKG 420

Query: 421 GWFRSGDLGVKHPDGYIELKDRSKDIIISGGENISTIEVESVLFSHPSVLDAAVVGRPDD 480
           GWF SGDLGVKHPDGYIELKDRSKDIIISGGENISTIEVESVLFSHP +L+AA+VGRPD+
Sbjct: 421 GWFHSGDLGVKHPDGYIELKDRSKDIIISGGENISTIEVESVLFSHPDILEAAIVGRPDE 480

Query: 481 HWGETPCAFVKLKDGCSATEGEIIKFCREHLPHYMAPRSVVFRDLPKTSTGKTQKFILKK 540
           +WGETPCAFVKLKDGC     EIIKFC++ LPHYMAPR+VVF DLPKTSTGK QKF+L++
Sbjct: 481 YWGETPCAFVKLKDGCKTGREEIIKFCKDRLPHYMAPRTVVFEDLPKTSTGKVQKFVLRE 540

Query: 541 EAKAMGSLPKR-VSKL 556
           +AKAMGSL K  +SKL
Sbjct: 541 KAKAMGSLTKNTISKL 554

BLAST of CSPI04G26850 vs. TrEMBL
Match: B9H5H2_POPTR (AMP-dependent synthetase and ligase family protein OS=Populus trichocarpa GN=POPTR_0005s27210g PE=4 SV=1)

HSP 1 Score: 852.4 bits (2201), Expect = 3.0e-244
Identity = 399/550 (72.55%), Postives = 473/550 (86.00%), Query Frame = 1

Query: 1   MDGSHRCSANYVPLTPISFLERSAAVYGDRISLVYGRVQYTWRDTLQRCTRLASALVRTG 60
           M+G  +CSANYVPLTPISFLERSA VY DR+S+ YG ++YTW++T +RC RLASAL   G
Sbjct: 1   MEGMVKCSANYVPLTPISFLERSAIVYRDRVSVAYGDIKYTWKETHERCVRLASALAHLG 60

Query: 61  IARGDVVAALVPNIPAMYELHFAVPMAGAVLCSLNTRHDAAMVSTLLSHSEAKIIVVDYQ 120
           I+ GDVVAAL PNIPAMYELHF VPMAGAVLC+LN RHD++MVS LL HSEAK+I VDYQ
Sbjct: 61  ISPGDVVAALAPNIPAMYELHFGVPMAGAVLCTLNVRHDSSMVSVLLKHSEAKLIFVDYQ 120

Query: 121 LEHIVTGAIKAMSERKEKLPRVVIIQEYDQPPSRIDRPGSALEYLEFESFLASGKLNFEI 180
              I  GA++ +SERK KLP +V+I E  QP +      S+   LE+ES L  GKL+FE+
Sbjct: 121 CLPIAQGALEILSERKTKLPLLVLIPECGQPAAI-----SSPRILEYESLLEKGKLDFEV 180

Query: 181 RRPRDELDPIALNYTSGTTSRPKGVIFSHRGAYLNSLSAVLLNDMCSLPVYLWTVPMFHC 240
           RRPRDE DPI+LNYTSGTTS PKGVI+SHRGAYLNSL+A LLNDM ++PVYLW VPMFHC
Sbjct: 181 RRPRDEWDPISLNYTSGTTSSPKGVIYSHRGAYLNSLAAALLNDMSAMPVYLWCVPMFHC 240

Query: 241 NGWCLTWGVAAQSGTNICQRNVTAKEIFDNISLHKVTHMGGAPTVSNMIINAPISEQKPL 300
           NGWCLTW VAAQ GTN+CQR VTA++IF+NI+ HKVTHM GAPTV NMIINA  SE++PL
Sbjct: 241 NGWCLTWAVAAQGGTNVCQRYVTARDIFENIAQHKVTHMSGAPTVLNMIINASASEKRPL 300

Query: 301 PREVTMMSGGAPPPSHVLYKLKALGFRIVHSYGLTETYGPATVCSWKPEWDSLPQDKQAK 360
           P +V +M+GGAPPPSHVL+K++ LGF + HSYGLTETYGP TVC+WKPEW SL  + QAK
Sbjct: 301 PGKVAVMTGGAPPPSHVLFKMEELGFHVTHSYGLTETYGPGTVCAWKPEWASLTPEAQAK 360

Query: 361 LNSRQGLQHIGLEAADVKDPVTMESVPADGKTMGEVMLRGNTVMSGYLQDLKATREAFNG 420
           + SRQG+QH+GLE  D+KDPVTM+SVPAD KTMGEVM RGNTVM+GYL++L+AT++AF+G
Sbjct: 361 IKSRQGVQHLGLEELDIKDPVTMKSVPADAKTMGEVMFRGNTVMNGYLKNLEATKDAFSG 420

Query: 421 GWFRSGDLGVKHPDGYIELKDRSKDIIISGGENISTIEVESVLFSHPSVLDAAVVGRPDD 480
           GWFRSGDLGVKHPDGYIELKDRSKDIIISGGENIS+IEVES LFSHP+VL+AA+VGRPDD
Sbjct: 421 GWFRSGDLGVKHPDGYIELKDRSKDIIISGGENISSIEVESALFSHPAVLEAAIVGRPDD 480

Query: 481 HWGETPCAFVKLKDGCSATEGEIIKFCREHLPHYMAPRSVVFRDLPKTSTGKTQKFILKK 540
           +WGETPCAFVKLK+GC+A   E+IKFCR+HLPHYM PR+VVF++LPKTSTGK QK++LK+
Sbjct: 481 YWGETPCAFVKLKEGCNANAEELIKFCRDHLPHYMTPRTVVFQELPKTSTGKVQKYVLKE 540

Query: 541 EAKAMGSLPK 551
           +AKAMGS+ K
Sbjct: 541 KAKAMGSISK 545

BLAST of CSPI04G26850 vs. TAIR10
Match: AT1G20560.1 (AT1G20560.1 acyl activating enzyme 1)

HSP 1 Score: 780.8 bits (2015), Expect = 5.6e-226
Identity = 365/551 (66.24%), Postives = 448/551 (81.31%), Query Frame = 1

Query: 1   MDGSHRCSANYVPLTPISFLERSAAVYGDRISLVYGRVQYTWRDTLQRCTRLASALVRTG 60
           M+G+ +  ANYVPLTPISFL+RSA VY DR+S+VYG V+YTWR T  RC R+ASAL + G
Sbjct: 3   MEGTIKSPANYVPLTPISFLDRSAVVYADRVSIVYGSVKYTWRQTRDRCVRIASALSQLG 62

Query: 61  IARGDVVAALVPNIPAMYELHFAVPMAGAVLCSLNTRHDAAMVSTLLSHSEAKIIVVDYQ 120
           I+ GDVV+ L PN+PAM ELHF VPMAGA+LC+LN RHD+++V+ LL HS  K+I  D+Q
Sbjct: 63  ISTGDVVSVLAPNVPAMVELHFGVPMAGALLCTLNIRHDSSLVAVLLRHSGTKVIFADHQ 122

Query: 121 LEHIVTGAIKAMSERKEKLPRVVIIQEYDQPPSRIDRPGSALEYLEFESFLASGKLNFEI 180
              I  GA + +S + +K+P +V+I E       + R   + E +E+E  +A GK +FE+
Sbjct: 123 FLQIAEGACEILSNKGDKVPILVLIPE--PLTQSVSRKKRSEEMMEYEDVVAMGKSDFEV 182

Query: 181 RRPRDELDPIALNYTSGTTSRPKGVIFSHRGAYLNSLSAVLLNDMCSLPVYLWTVPMFHC 240
            RP DE D I++NYTSGTTS PKGV++SHRGAYLNSL+AVLLN+M S P YLWT PMFHC
Sbjct: 183 IRPTDECDAISVNYTSGTTSSPKGVVYSHRGAYLNSLAAVLLNEMHSSPTYLWTNPMFHC 242

Query: 241 NGWCLTWGVAAQSGTNICQRNVTAKEIFDNISLHKVTHMGGAPTVSNMIINAPISEQKPL 300
           NGWCL WGV A  GTNIC RNVTAK IFDNIS HKVTHMGGAPT+ NMIINAP SEQKPL
Sbjct: 243 NGWCLLWGVTAIGGTNICLRNVTAKAIFDNISQHKVTHMGGAPTILNMIINAPESEQKPL 302

Query: 301 PREVTMMSGGAPPPSHVLYKLKALGFRIVHSYGLTETYGPATVCSWKPEWDSLPQDKQAK 360
           P +V+ ++G APPP+HV++K++ LGF + HSYGLTETYGP T+C+WKPEWDSLP+++QAK
Sbjct: 303 PGKVSFITGAAPPPAHVIFKMEELGFSMFHSYGLTETYGPGTICTWKPEWDSLPREEQAK 362

Query: 361 LNSRQGLQHIGLEAADVKDPVTMESVPADGKTMGEVMLRGNTVMSGYLQDLKATREAFNG 420
           + +RQG+ H+GLE   VKDPVTM ++PADG TMGEV+ RGNTVM+GYL++ +AT+EAF G
Sbjct: 363 MKARQGVNHLGLEEIQVKDPVTMRTLPADGVTMGEVVFRGNTVMNGYLKNPEATKEAFKG 422

Query: 421 GWFRSGDLGVKHPDGYIELKDRSKDIIISGGENISTIEVESVLFSHPSVLDAAVVGRPDD 480
           GWF SGDLGVKHPDGYIELKDRSKDIIISGGENIS+IEVES LF+HP VL+AAVV RPD+
Sbjct: 423 GWFWSGDLGVKHPDGYIELKDRSKDIIISGGENISSIEVESTLFTHPCVLEAAVVARPDE 482

Query: 481 HWGETPCAFVKLKDGCSATEGEIIKFCREHLPHYMAPRSVVFRDLPKTSTGKTQKFILKK 540
           +WGET CAFVKLKDG  A+  E+I +CR+ LPHYMAPRS+VF DLPKTSTGK QKF+L+ 
Sbjct: 483 YWGETACAFVKLKDGSKASAEELISYCRDRLPHYMAPRSIVFEDLPKTSTGKVQKFVLRT 542

Query: 541 EAKAMGSLPKR 552
           +AKA+ SL K+
Sbjct: 543 KAKALVSLSKK 551

BLAST of CSPI04G26850 vs. TAIR10
Match: AT2G17650.1 (AT2G17650.1 AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 726.9 bits (1875), Expect = 9.7e-210
Identity = 352/556 (63.31%), Postives = 431/556 (77.52%), Query Frame = 1

Query: 1   MDGSHRCSANYVPLTPISFLERSAAVYGDRISLVYGRVQYTWRDTLQRCTRLASALVRTG 60
           ++G  R  AN+ PL+PI+FLERSA VY DR SLV+G V++TW  T QRC RLASAL   G
Sbjct: 48  IEGLLRSPANFSPLSPITFLERSAKVYRDRTSLVFGSVKHTWFQTYQRCLRLASALTNLG 107

Query: 61  IARGDVVAALVPNIPAMYELHFAVPMAGAVLCSLNTRHDAAMVSTLLSHSEAKIIVVDYQ 120
           I+RGDVVAAL PN+PAM+ELHFAVPMAG +LC LNTR D + +S LL+HSEAKI+ VD+Q
Sbjct: 108 ISRGDVVAALAPNVPAMHELHFAVPMAGLILCPLNTRLDPSTLSVLLAHSEAKILFVDHQ 167

Query: 121 LEHIVTGAIKAMSER---KEKLPRVVIIQEYDQPPSRIDRPGS-ALEY---LEFESFLAS 180
           L  I  GA+  +++    ++ L  V+I Q  D   S  D   + A +Y    E+E+ L S
Sbjct: 168 LLEIAHGALDLLAKSDKTRKSLKLVLISQSNDDDDSDEDSSSTFASKYSFDYEYETLLKS 227

Query: 181 GKLNFEIRRPRDELDPIALNYTSGTTSRPKGVIFSHRGAYLNSLSAVLLNDMCSLPVYLW 240
           G   FEI +PR E DPI++NYTSGTTSRPKGV++SHRGAYLNSL+ V L+ M   PVYLW
Sbjct: 228 GDSEFEIIKPRCEWDPISINYTSGTTSRPKGVVYSHRGAYLNSLATVFLHQMSVYPVYLW 287

Query: 241 TVPMFHCNGWCLTWGVAAQSGTNICQRNVTAKEIFDNISLHKVTHMGGAPTVSNMIINAP 300
           TVPMFHCNGWCL WGVAAQ GTNIC R V+ K IF NI++HKVTHMGGAPTV NMI+N  
Sbjct: 288 TVPMFHCNGWCLVWGVAAQGGTNICLRKVSPKMIFKNIAMHKVTHMGGAPTVLNMIVNYT 347

Query: 301 ISEQKPLPREVTMMSGGAPPPSHVLYKLKALGFRIVHSYGLTETYGPATVCSWKPEWDSL 360
           ++E KPLP  V +M+GG+PP   +L K++ LGF + H YGLTETYGP T C WKPEWDSL
Sbjct: 348 VTEHKPLPHRVEIMTGGSPPLPQILAKMEELGFNVSHLYGLTETYGPGTHCVWKPEWDSL 407

Query: 361 PQDKQAKLNSRQGLQHIGLEAADVKDPVTMESVPADGKTMGEVMLRGNTVMSGYLQDLKA 420
             +++ KL +RQG+QH+GLE  DVKDP+TME+VP DG TMGEVM RGNTVMSGY +D++A
Sbjct: 408 SLEERTKLKARQGVQHLGLEGLDVKDPLTMETVPDDGLTMGEVMFRGNTVMSGYFKDIEA 467

Query: 421 TREAFNGGWFRSGDLGVKHPDGYIELKDRSKDIIISGGENISTIEVESVLFSHPSVLDAA 480
           TR+AF G WF SGDL VK+PDGYIE+KDR KD+IISGGENIS++EVE VL SH +VL+AA
Sbjct: 468 TRKAFEGDWFHSGDLAVKYPDGYIEIKDRLKDVIISGGENISSVEVERVLCSHQAVLEAA 527

Query: 481 VVGRPDDHWGETPCAFVKLKDGCSATE-GEIIKFCREHLPHYMAPRSVVFRDLPKTSTGK 540
           VV RPD HWG+TPC FVKLK+G    +  EII FCR+HLPHYMAP+++VF D+PKTSTGK
Sbjct: 528 VVARPDHHWGQTPCGFVKLKEGFDTIKPEEIIGFCRDHLPHYMAPKTIVFGDIPKTSTGK 587

Query: 541 TQKFILKKEAKAMGSL 549
            QK++L+K+A  MGSL
Sbjct: 588 VQKYLLRKKADEMGSL 603

BLAST of CSPI04G26850 vs. TAIR10
Match: AT3G16910.1 (AT3G16910.1 acyl-activating enzyme 7)

HSP 1 Score: 596.3 bits (1536), Expect = 2.0e-170
Identity = 293/559 (52.42%), Postives = 387/559 (69.23%), Query Frame = 1

Query: 1   MDGSHRCSANYVPLTPISFLERSAAVYGDRISLVYGRVQYTWRDTLQRCTRLASALVRTG 60
           +D   +  ANY  LTP+ FL+R+A V+  R S+++G  +YTWR T  RC RLASAL    
Sbjct: 9   IDDLPKIPANYTALTPLWFLDRAAVVHPTRKSVIHGSREYTWRQTYDRCRRLASALADRS 68

Query: 61  IARGDVVAALVPNIPAMYELHFAVPMAGAVLCSLNTRHDAAMVSTLLSHSEAKIIVVDYQ 120
           I  G  VA + PNIPAMYE HF VPM GAVL  +N R +A  V+ LLSHS++ +I+VD +
Sbjct: 69  IGPGSTVAIIAPNIPAMYEAHFGVPMCGAVLNCVNIRLNAPTVAFLLSHSQSSVIMVDQE 128

Query: 121 LEHIVTGAIKAMSERKE---KLPRVVIIQEYDQPPSRIDRPGSALEYLEFESFLASGKLN 180
              +   +++ M E+     K P +++I ++   P  ++R  S    +E+E FLA+G  N
Sbjct: 129 FFTLAEDSLRLMEEKAGSSFKRPLLIVIGDHTCAPESLNRALSK-GAIEYEDFLATGDPN 188

Query: 181 FEIRRPRDELDPIALNYTSGTTSRPKGVIFSHRGAYLNSLSAVLLNDMCSLPVYLWTVPM 240
           +  + P DE   IAL YTSGTT+ PKGV+  HRGAY+ +LS  L+  M    VYLWT+PM
Sbjct: 189 YPWQPPADEWQSIALGYTSGTTASPKGVVLHHRGAYIMALSNPLIWGMQDGAVYLWTLPM 248

Query: 241 FHCNGWCLTWGVAAQSGTNICQRNVTAKEIFDNISLHKVTHMGGAPTVSNMIINAPISEQ 300
           FHCNGWC  W +A  SGT+IC R VTAKE++  I+ +KVTH   AP V N I+NAP  + 
Sbjct: 249 FHCNGWCFPWSLAVLSGTSICLRQVTAKEVYSMIAKYKVTHFCAAPVVLNAIVNAPKEDT 308

Query: 301 -KPLPREVTMMSGGAPPPSHVLYKLKALGFRIVHSYGLTETYGPATVCSWKPEWDSLPQD 360
             PLP  V +M+ GA PP  VL+ +   GFR+ H+YGL+ETYGP+TVC+WKPEWDSLP +
Sbjct: 309 ILPLPHTVHVMTAGAAPPPSVLFSMNQKGFRVAHTYGLSETYGPSTVCAWKPEWDSLPPE 368

Query: 361 KQAKLNSRQGLQHIGLEAADVKDPVTMESVPADGKTMGEVMLRGNTVMSGYLQDLKATRE 420
            QAKLN+RQG+++ G+E  DV D  T + VPADGKT GE++ RGN VM GYL++ +A +E
Sbjct: 369 TQAKLNARQGVRYTGMEQLDVIDTQTGKPVPADGKTAGEIVFRGNMVMKGYLKNPEANKE 428

Query: 421 AFNGGWFRSGDLGVKHPDGYIELKDRSKDIIISGGENISTIEVESVLFSHPSVLDAAVVG 480
            F GGWF SGD+ VKHPD YIE+KDRSKD+IISGGENIS++EVE+V++ HP+VL+A+VV 
Sbjct: 429 TFAGGWFHSGDIAVKHPDNYIEIKDRSKDVIISGGENISSVEVENVVYHHPAVLEASVVA 488

Query: 481 RPDDHWGETPCAFVKLKDGCSATE-----GEIIKFCREHLPHYMAPRSVVFRDLPKTSTG 540
           RPD+ W E+PCAFV LK      +      +I+KFCRE LP Y  P+SVVF  LPKT+TG
Sbjct: 489 RPDERWQESPCAFVTLKSDYEKHDQNKLAQDIMKFCREKLPAYWVPKSVVFGPLPKTATG 548

Query: 541 KTQKFILKKEAKAMGSLPK 551
           K QK IL+ +AK MG +P+
Sbjct: 549 KIQKHILRTKAKEMGPVPR 566

BLAST of CSPI04G26850 vs. TAIR10
Match: AT1G76290.1 (AT1G76290.1 AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 586.6 bits (1511), Expect = 1.6e-167
Identity = 290/554 (52.35%), Postives = 383/554 (69.13%), Query Frame = 1

Query: 1   MDGSHRCSANYVPLTPISFLERSAAVYGDRISLVYGRVQYTWRDTLQRCTRLASALVRTG 60
           M+G+ RCSANYVPL+PISFLER+A V+G R S+VYG +QYTW  T  RC RLASAL   G
Sbjct: 1   MEGTMRCSANYVPLSPISFLERAAVVFGSRTSVVYGDIQYTWHQTRDRCVRLASALSDLG 60

Query: 61  IARGDVVAALVPNIPAMYELHFAVPMAGAVLCSLNTRHDAAMVSTLLSHSEAKIIVVDYQ 120
           ++R DVVAAL PN+PA+ EL+F  PMAGAVLC LNT  D+ M++  L  ++ K+  VD +
Sbjct: 61  LSRHDVVAALAPNVPALCELYFGAPMAGAVLCVLNTTFDSQMLAMALEKTKPKVFFVDSE 120

Query: 121 LEHIVTGAIKAMSERKEKLPRVVIIQEYDQPPSRIDRPGSALEYLEFESFLASGKLNFEI 180
              +   ++  +S  +EK P ++ I E          P    +Y ++E FL++G  NF+ 
Sbjct: 121 FLSVAEESLSLLSNIEEK-PLIITITE---------NPTEQSKYEQYEDFLSTGNPNFKP 180

Query: 181 RRPRDELDPIALNYTSGTTSRPKGVIFSHRGAYLNSLSAVLLNDMCSLPVYLWTVPMFHC 240
            RP DE DPIALN+TSGTTS PK V++SHRGAYLN+ +  ++N+M  +PVYL TVPM+HC
Sbjct: 181 IRPVDECDPIALNFTSGTTSTPKCVVYSHRGAYLNATAVGVMNEMKPMPVYLCTVPMYHC 240

Query: 241 NGWCLTWGVAAQSGTNICQRNVTAKEIFDNISLHKVTHMGGAPTVSNMIINAPISEQKPL 300
           +GWC  W V A  G  +C R V  + IFD+I  HKVT+ GG+P V NMI NA  S +K  
Sbjct: 241 SGWCYIWTVTAFGGVIVCLREVNDEVIFDSIVKHKVTNFGGSPPVLNMIANARDSVKKSF 300

Query: 301 PREVTMMSGGAPPPSHVLYKLKALGFRIVHSYGLTETYGPATVCSWKPEWDSLPQDKQAK 360
           P  V +MSGG+ PP  V+ KLK LGF+++ +YG +E YG  T C W PEW++LP+++  +
Sbjct: 301 PWTVQVMSGGSSPPE-VMLKLKKLGFKVMMAYGCSEVYGLGTACLWMPEWETLPEEESLR 360

Query: 361 LNSRQGLQHIGLEAADVKDPVTMESVPADGKTMGEVMLRGNTVMSGYLQDLKATREAFNG 420
           L +R GL H   EA DV DP TM+SVP DGKT+  + LRGNTVMSGY +D +AT  AF G
Sbjct: 361 LKARDGLNHFAKEAVDVLDPTTMKSVPHDGKTIRVIALRGNTVMSGYFKDKEATEAAFRG 420

Query: 421 GWFRSGDLGVKHPDGYIELKDRSKDIIISGGENISTIEVESVLFSHPSVLDAAVVGRPDD 480
           GW+ S D+GV  PDGYI+ KDRS+D+I  GGE + + E+E +L+SHP+V DA VVGRPD+
Sbjct: 421 GWYWSRDMGVIDPDGYIQFKDRSQDVITCGGEIVGSKEIEGILYSHPAVYDAGVVGRPDE 480

Query: 481 HWGETPCAFVKLKDGCSATEGEIIKFCREHLPH---YMAPRSVVFRDLPKTSTGKTQKFI 540
             GE+ CAFVKLK+G  A E EII+FC+  L +    M P++VVF D+PKT TGK +K +
Sbjct: 481 TLGESMCAFVKLKEGAEAREEEIIEFCKRKLGNKNMKMIPKTVVFSDVPKTPTGKIRKNV 540

Query: 541 LKKEAKAMGSLPKR 552
           L+K AK MG +  R
Sbjct: 541 LRKMAKDMGYVQLR 543

BLAST of CSPI04G26850 vs. TAIR10
Match: AT1G66120.1 (AT1G66120.1 AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 569.7 bits (1467), Expect = 2.0e-162
Identity = 272/554 (49.10%), Postives = 386/554 (69.68%), Query Frame = 1

Query: 1   MDGSHRCSANYVPLTPISFLERSAAVYGDRISLVYGRVQYTWRDTLQRCTRLASALVRTG 60
           MD    C AN VPLTPI+FL+R++  Y +R S++YG+ ++TW  T  RC RLA++L+   
Sbjct: 1   MDNLVLCEANNVPLTPITFLKRASECYPNRTSIIYGQTRFTWPQTYDRCCRLAASLLSLN 60

Query: 61  IARGDVVAALVPNIPAMYELHFAVPMAGAVLCSLNTRHDAAMVSTLLSHSEAKIIVVDYQ 120
           I R DVV+ L PN+PAMYE+HF+VPM GAVL  +NTR DA  ++ +L H+E KI+ VDY+
Sbjct: 61  ITRNDVVSILAPNVPAMYEMHFSVPMTGAVLNPINTRLDAKTIAIILRHAEPKILFVDYE 120

Query: 121 LEHIVTGAIKAM-SERKEKLPRVVIIQEYDQPPSRIDRPGSALEYLEFESFLASGK---- 180
              ++   ++ + + + +  PR+++I E D       +       L++E  +  G+    
Sbjct: 121 FAPLIQEVLRLIPTYQSQPHPRIILINEIDSTTKPFSKE------LDYEGLIRKGEPTPS 180

Query: 181 LNFEIRRPRDELDPIALNYTSGTTSRPKGVIFSHRGAYLNSLSAVLLNDMCSLPVYLWTV 240
            +  + R  +E DPI+LNYTSGTT+ PKGV+ SH+GAYL++LS+++  +M   PVYLWT+
Sbjct: 181 SSASMFRVHNEHDPISLNYTSGTTADPKGVVISHQGAYLSALSSIIGWEMGIFPVYLWTL 240

Query: 241 PMFHCNGWCLTWGVAAQSGTNICQRNVTAKEIFDNISLHKVTHMGGAPTVSNMIINAPIS 300
           PMFHCNGW  TW VAA+ GTN+C R+VTA EI+ NI LH VTHM   PTV   ++    +
Sbjct: 241 PMFHCNGWTHTWSVAARGGTNVCIRHVTAPEIYKNIELHGVTHMSCVPTVFRFLLEGSRT 300

Query: 301 EQKPLPREVTMMSGGAPPPSHVLYKLKALGFRIVHSYGLTETYGPATVCSWKPEWDSLPQ 360
           +Q P    V +++GG+ PP+ ++ K++ LGF ++H YGLTE  GP   C W+ EW+ LP+
Sbjct: 301 DQSPKSSPVQVLTGGSSPPAVLIKKVEQLGFHVMHGYGLTEATGPVLFCEWQDEWNKLPE 360

Query: 361 DKQAKLNSRQGLQHIGLEAADVKDPVTMESVPADGKTMGEVMLRGNTVMSGYLQDLKATR 420
            +Q +L  RQG++++ L   DVK+  T+ESVP DGKTMGE++++G+++M GYL++ KAT 
Sbjct: 361 HQQIELQQRQGVRNLTLADVDVKNTKTLESVPRDGKTMGEIVIKGSSLMKGYLKNPKATS 420

Query: 421 EAFNGGWFRSGDLGVKHPDGYIELKDRSKDIIISGGENISTIEVESVLFSHPSVLDAAVV 480
           EAF  GW  +GD+GV HPDGY+E+KDRSKDIIISGGENIS+IEVE VL+ +  VL+AAVV
Sbjct: 421 EAFKHGWLNTGDIGVIHPDGYVEIKDRSKDIIISGGENISSIEVEKVLYMYQEVLEAAVV 480

Query: 481 GRPDDHWGETPCAFVKLK---DGCSATEGEIIKFCREHLPHYMAPRSVV-FRDLPKTSTG 540
             P   WGETPCAFV LK   +G   +EG++IK+CRE++PH+M P+ VV F++LPK S G
Sbjct: 481 AMPHPLWGETPCAFVVLKKGEEGLVTSEGDLIKYCRENMPHFMCPKKVVFFQELPKNSNG 540

Query: 541 KTQKFILKKEAKAM 546
           K  K  L+  AKA+
Sbjct: 541 KILKSKLRDIAKAL 548

BLAST of CSPI04G26850 vs. NCBI nr
Match: gi|449455591|ref|XP_004145536.1| (PREDICTED: probable acyl-activating enzyme 1, peroxisomal [Cucumis sativus])

HSP 1 Score: 1129.4 bits (2920), Expect = 0.0e+00
Identity = 554/555 (99.82%), Postives = 555/555 (100.00%), Query Frame = 1

Query: 1   MDGSHRCSANYVPLTPISFLERSAAVYGDRISLVYGRVQYTWRDTLQRCTRLASALVRTG 60
           MDGSHRCSANYVPLTPISFLERSAAVYGDRISLVYGRVQYTWRDTLQRCTRLASALVRTG
Sbjct: 1   MDGSHRCSANYVPLTPISFLERSAAVYGDRISLVYGRVQYTWRDTLQRCTRLASALVRTG 60

Query: 61  IARGDVVAALVPNIPAMYELHFAVPMAGAVLCSLNTRHDAAMVSTLLSHSEAKIIVVDYQ 120
           IARGDVVAALVPNIPAMYELHFAVPMAGAVLCSLNTRHDAAMVSTLLSHSEAKIIVVDYQ
Sbjct: 61  IARGDVVAALVPNIPAMYELHFAVPMAGAVLCSLNTRHDAAMVSTLLSHSEAKIIVVDYQ 120

Query: 121 LEHIVTGAIKAMSERKEKLPRVVIIQEYDQPPSRIDRPGSALEYLEFESFLASGKLNFEI 180
           LEHIVTGAIKAMSERKEKLPRVVIIQEYDQPPSRIDRPGSALEYLEFESFLASGKLNFEI
Sbjct: 121 LEHIVTGAIKAMSERKEKLPRVVIIQEYDQPPSRIDRPGSALEYLEFESFLASGKLNFEI 180

Query: 181 RRPRDELDPIALNYTSGTTSRPKGVIFSHRGAYLNSLSAVLLNDMCSLPVYLWTVPMFHC 240
           RRPRDELDPIALNYTSGTTSRPKGVIFSHRGAYLNSLSAVLLNDMCSLPVYLWTVPMFHC
Sbjct: 181 RRPRDELDPIALNYTSGTTSRPKGVIFSHRGAYLNSLSAVLLNDMCSLPVYLWTVPMFHC 240

Query: 241 NGWCLTWGVAAQSGTNICQRNVTAKEIFDNISLHKVTHMGGAPTVSNMIINAPISEQKPL 300
           NGWCLTWGVAAQSGTNICQRNVTAKEIFDNISLHKVTHMGGAPTVSNMIINAPISEQKPL
Sbjct: 241 NGWCLTWGVAAQSGTNICQRNVTAKEIFDNISLHKVTHMGGAPTVSNMIINAPISEQKPL 300

Query: 301 PREVTMMSGGAPPPSHVLYKLKALGFRIVHSYGLTETYGPATVCSWKPEWDSLPQDKQAK 360
           PREVTMMSGGAPPPSHVLYKLKALGFRIVHSYGLTETYGPATVCSWKPEWDSLPQDKQAK
Sbjct: 301 PREVTMMSGGAPPPSHVLYKLKALGFRIVHSYGLTETYGPATVCSWKPEWDSLPQDKQAK 360

Query: 361 LNSRQGLQHIGLEAADVKDPVTMESVPADGKTMGEVMLRGNTVMSGYLQDLKATREAFNG 420
           LNSRQGLQHIGLEAADVKDPVTMESVPADGKTMGEVMLRGNTVMSGYL+DLKATREAFNG
Sbjct: 361 LNSRQGLQHIGLEAADVKDPVTMESVPADGKTMGEVMLRGNTVMSGYLKDLKATREAFNG 420

Query: 421 GWFRSGDLGVKHPDGYIELKDRSKDIIISGGENISTIEVESVLFSHPSVLDAAVVGRPDD 480
           GWFRSGDLGVKHPDGYIELKDRSKDIIISGGENISTIEVESVLFSHPSVLDAAVVGRPDD
Sbjct: 421 GWFRSGDLGVKHPDGYIELKDRSKDIIISGGENISTIEVESVLFSHPSVLDAAVVGRPDD 480

Query: 481 HWGETPCAFVKLKDGCSATEGEIIKFCREHLPHYMAPRSVVFRDLPKTSTGKTQKFILKK 540
           HWGETPCAFVKLKDGCSATEGEIIKFCREHLPHYMAPRSVVFRDLPKTSTGKTQKFILKK
Sbjct: 481 HWGETPCAFVKLKDGCSATEGEIIKFCREHLPHYMAPRSVVFRDLPKTSTGKTQKFILKK 540

Query: 541 EAKAMGSLPKRVSKL 556
           EAKAMGSLPKRVSKL
Sbjct: 541 EAKAMGSLPKRVSKL 555

BLAST of CSPI04G26850 vs. NCBI nr
Match: gi|659104645|ref|XP_008452947.1| (PREDICTED: probable acyl-activating enzyme 1, peroxisomal [Cucumis melo])

HSP 1 Score: 1083.2 bits (2800), Expect = 0.0e+00
Identity = 530/555 (95.50%), Postives = 543/555 (97.84%), Query Frame = 1

Query: 1   MDGSHRCSANYVPLTPISFLERSAAVYGDRISLVYGRVQYTWRDTLQRCTRLASALVRTG 60
           MDG HRCSANYVPLTPI+FLERSAAVYGDRISLVYGRVQYTWRDTL+RCTRLASALVRTG
Sbjct: 1   MDGIHRCSANYVPLTPITFLERSAAVYGDRISLVYGRVQYTWRDTLERCTRLASALVRTG 60

Query: 61  IARGDVVAALVPNIPAMYELHFAVPMAGAVLCSLNTRHDAAMVSTLLSHSEAKIIVVDYQ 120
           IARGDVVAALVPNIPAMYELHFAVPMAGAVLCSLNTRHDAAMVSTLLSHSEAKIIVVDYQ
Sbjct: 61  IARGDVVAALVPNIPAMYELHFAVPMAGAVLCSLNTRHDAAMVSTLLSHSEAKIIVVDYQ 120

Query: 121 LEHIVTGAIKAMSERKEKLPRVVIIQEYDQPPSRIDRPGSALEYLEFESFLASGKLNFEI 180
           LEHIVTGAI  MSERKEKLPRVVIIQEYDQPPS IDRPGSALE   FESFLASG+L+FEI
Sbjct: 121 LEHIVTGAINTMSERKEKLPRVVIIQEYDQPPSHIDRPGSALE---FESFLASGELDFEI 180

Query: 181 RRPRDELDPIALNYTSGTTSRPKGVIFSHRGAYLNSLSAVLLNDMCSLPVYLWTVPMFHC 240
           RRPRDE DPIALNYTSGTTSRPKGVI+SHRGAYLN+LSAVLLNDMCSLPVYLWTVPMFHC
Sbjct: 181 RRPRDEWDPIALNYTSGTTSRPKGVIYSHRGAYLNALSAVLLNDMCSLPVYLWTVPMFHC 240

Query: 241 NGWCLTWGVAAQSGTNICQRNVTAKEIFDNISLHKVTHMGGAPTVSNMIINAPISEQKPL 300
           NGWCLTWGVAAQSGTNICQRNVTAKEIFDNISLHKVTHMGGAPTVSNMIINAPISEQKPL
Sbjct: 241 NGWCLTWGVAAQSGTNICQRNVTAKEIFDNISLHKVTHMGGAPTVSNMIINAPISEQKPL 300

Query: 301 PREVTMMSGGAPPPSHVLYKLKALGFRIVHSYGLTETYGPATVCSWKPEWDSLPQDKQAK 360
           PR+VTMMSGGAPPPSHVLYKLKALGFRIVHSYGLTETYGP TVCSWKPEWDSLPQDKQAK
Sbjct: 301 PRQVTMMSGGAPPPSHVLYKLKALGFRIVHSYGLTETYGPGTVCSWKPEWDSLPQDKQAK 360

Query: 361 LNSRQGLQHIGLEAADVKDPVTMESVPADGKTMGEVMLRGNTVMSGYLQDLKATREAFNG 420
           LNSRQGLQHIGLEA D+KD VTM+SVPADGKTMGEVMLRGNTVMSGYL+DLKAT+EAFNG
Sbjct: 361 LNSRQGLQHIGLEAVDIKDSVTMKSVPADGKTMGEVMLRGNTVMSGYLKDLKATQEAFNG 420

Query: 421 GWFRSGDLGVKHPDGYIELKDRSKDIIISGGENISTIEVESVLFSHPSVLDAAVVGRPDD 480
           GWFRSGDLGVKHPDGYIELKDRSKDIIISGGENISTIEVESVLFSHPSVLDAAVVGRPDD
Sbjct: 421 GWFRSGDLGVKHPDGYIELKDRSKDIIISGGENISTIEVESVLFSHPSVLDAAVVGRPDD 480

Query: 481 HWGETPCAFVKLKDGCSATEGEIIKFCREHLPHYMAPRSVVFRDLPKTSTGKTQKFILKK 540
           HWGETPCAF+KLKDGCSATE EIIKFCREHLPHYMAPRSVVFR+LPKTSTGKTQKFILKK
Sbjct: 481 HWGETPCAFLKLKDGCSATEEEIIKFCREHLPHYMAPRSVVFRELPKTSTGKTQKFILKK 540

Query: 541 EAKAMGSLPKRVSKL 556
           EAKAMGSLPKRVSKL
Sbjct: 541 EAKAMGSLPKRVSKL 552

BLAST of CSPI04G26850 vs. NCBI nr
Match: gi|659104647|ref|XP_008452948.1| (PREDICTED: probable acyl-activating enzyme 1, peroxisomal [Cucumis melo])

HSP 1 Score: 886.3 bits (2289), Expect = 2.7e-254
Identity = 425/551 (77.13%), Postives = 486/551 (88.20%), Query Frame = 1

Query: 1   MDGSHRCSANYVPLTPISFLERSAAVYGDRISLVYGRVQYTWRDTLQRCTRLASALVRTG 60
           M+G   CSAN +PLTPI+FLER+AAV+ DRISLVYG V++TW +TLQRCT+ ASALV  G
Sbjct: 1   MEGIIPCSANSLPLTPITFLERAAAVFADRISLVYGNVRFTWGETLQRCTKFASALVHLG 60

Query: 61  IARGDVVAALVPNIPAMYELHFAVPMAGAVLCSLNTRHDAAMVSTLLSHSEAKIIVVDYQ 120
           I+RGDVVA L PN+PA YELHFAVPMAGA+LC+LN RHDAAM+STLL HSEAKIIVVD+Q
Sbjct: 61  ISRGDVVAVLAPNVPATYELHFAVPMAGAILCTLNMRHDAAMISTLLGHSEAKIIVVDHQ 120

Query: 121 LEHIVTGAIKAMSERK--EKLPRVVIIQEYDQPPSRIDRPGSALEYLEFESFLASGKLNF 180
             HIV  AI+ MS+    E+LPR+VI+QE+D P S I+   SA + LE+ES L +G L+F
Sbjct: 121 YLHIVKAAIEIMSKMTGAEELPRIVIVQEFDHPSSNINGFDSASDDLEYESLLNTGTLDF 180

Query: 181 EIRRPRDELDPIALNYTSGTTSRPKGVIFSHRGAYLNSLSAVLLNDMCSLPVYLWTVPMF 240
           EIRRP DE DPI+LNYTSGTTSRPKGVI+SHRG YLN+LS +LLNDM S+PVYLWTVPMF
Sbjct: 181 EIRRPIDEWDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTILLNDMSSMPVYLWTVPMF 240

Query: 241 HCNGWCLTWGVAAQSGTNICQRNVTAKEIFDNISLHKVTHMGGAPTVSNMIINAPISEQK 300
           HCNGW +TWGVAAQ GTNICQRNV AKEIF NISLHKVTHMGGAPTV NMI+NAPI+EQK
Sbjct: 241 HCNGWSMTWGVAAQGGTNICQRNVNAKEIFANISLHKVTHMGGAPTVLNMIVNAPITEQK 300

Query: 301 PLPREVTMMSGGAPPPSHVLYKLKALGFRIVHSYGLTETYGPATVCSWKPEWDSLPQDKQ 360
           PLP +VT+M+GGAPPPS++LYK++ LGF IVH YGLTET G AT C WKPEWDSLP DKQ
Sbjct: 301 PLPGKVTVMTGGAPPPSNILYKMRELGFLIVHCYGLTETCGVATFCQWKPEWDSLPDDKQ 360

Query: 361 AKLNSRQGLQHIGLEAADVKDPVTMESVPADGKTMGEVMLRGNTVMSGYLQDLKATREAF 420
           AKL SRQG+QHIG+E  D+K+P+TMESVPADGKTMGEVM+RGNTVMSGYL+DLKAT EAF
Sbjct: 361 AKLKSRQGMQHIGVEGLDIKNPITMESVPADGKTMGEVMIRGNTVMSGYLKDLKATEEAF 420

Query: 421 NGGWFRSGDLGVKHPDGYIELKDRSKDIIISGGENISTIEVESVLFSHPSVLDAAVVGRP 480
           NGGWFRSGDLGV+HPDGYIELKDRSKDIIISGGENISTIEVESVLF+HPSVL+AA+VGRP
Sbjct: 421 NGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISTIEVESVLFNHPSVLEAAIVGRP 480

Query: 481 DDHWGETPCAFVKLKDGCSATEGEIIKFCREHLPHYMAPRSVVFR-DLPKTSTGKTQKFI 540
           DDHWGETPCAFV LKDG +AT+ EII+FCR+ LPHYMAP++VVF+ DLPK+STGK QK I
Sbjct: 481 DDHWGETPCAFVNLKDGSNATQEEIIEFCRDKLPHYMAPKTVVFKSDLPKSSTGKIQKLI 540

Query: 541 LKKEAKAMGSL 549
           LK+EAKAMGSL
Sbjct: 541 LKEEAKAMGSL 551

BLAST of CSPI04G26850 vs. NCBI nr
Match: gi|449455718|ref|XP_004145598.1| (PREDICTED: probable acyl-activating enzyme 1, peroxisomal [Cucumis sativus])

HSP 1 Score: 880.9 bits (2275), Expect = 1.1e-252
Identity = 424/553 (76.67%), Postives = 485/553 (87.70%), Query Frame = 1

Query: 6   RCSANYVPLTPISFLERSAAVYGDRISLVYGRVQYTWRDTLQRCTRLASALVRTGIARGD 65
           RCSAN VPLTPI+FLER+AAV+GDRISLVYG V++TWR+TLQRCT+ ASALV  GI+RGD
Sbjct: 5   RCSANSVPLTPITFLERAAAVFGDRISLVYGNVRFTWRETLQRCTKFASALVHVGISRGD 64

Query: 66  VVAALVPNIPAMYELHFAVPMAGAVLCSLNTRHDAAMVSTLLSHSEAKIIVVDYQLEHIV 125
           VVA L PN+PA YELHFAVPMAGA+LC+LN RHDAAMVSTLL HSEAKIIV D+Q  HIV
Sbjct: 65  VVAVLAPNVPATYELHFAVPMAGAILCTLNMRHDAAMVSTLLGHSEAKIIVADHQYLHIV 124

Query: 126 TGAIKAMSERK--EKLPRVVIIQEYDQPPSRIDRPGSALEYLEFESFLASGKLNFEIRRP 185
             AI+ MS+    E+LPR+VI+QE+D P S I+   SA + LE+ES L +G L+FEIRRP
Sbjct: 125 KAAIEIMSKTMAAEELPRIVIVQEFDHPSSNINGFDSASDDLEYESLLNTGTLDFEIRRP 184

Query: 186 RDELDPIALNYTSGTTSRPKGVIFSHRGAYLNSLSAVLLNDMCSLPVYLWTVPMFHCNGW 245
            DE DPI+LNYTSGTTSRPKGVI+SHRG YLN+LS VLLNDM S+ VYLWTVPMFHCNGW
Sbjct: 185 IDERDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTVLLNDMSSMAVYLWTVPMFHCNGW 244

Query: 246 CLTWGVAAQSGTNICQRNVTAKEIFDNISLHKVTHMGGAPTVSNMIINAPISEQKPLPRE 305
            +TWGVAAQ GTNICQRNV AKEIF NISLH VTHMGGAPTV NMI+NAPI+EQKPL  +
Sbjct: 245 SMTWGVAAQGGTNICQRNVNAKEIFANISLHNVTHMGGAPTVLNMIVNAPITEQKPLLGK 304

Query: 306 VTMMSGGAPPPSHVLYKLKALGFRIVHSYGLTETYGPATVCSWKPEWDSLPQDKQAKLNS 365
           VT+M+GGAPPPS++LYK++ LGF IVH YGLTETYG AT C WKPEWDSLP +KQAKL S
Sbjct: 305 VTVMTGGAPPPSNILYKMRELGFLIVHCYGLTETYGVATFCHWKPEWDSLPDEKQAKLKS 364

Query: 366 RQGLQHIGLEAADVKDPVTMESVPADGKTMGEVMLRGNTVMSGYLQDLKATREAFNGGWF 425
           RQG+QHIG+E  D+K+P+TMESVPADGKTMGEVM+RGNTVM GYL+D+KAT EAFNGGWF
Sbjct: 365 RQGMQHIGVEGVDIKNPITMESVPADGKTMGEVMIRGNTVMIGYLKDVKATEEAFNGGWF 424

Query: 426 RSGDLGVKHPDGYIELKDRSKDIIISGGENISTIEVESVLFSHPSVLDAAVVGRPDDHWG 485
           RSGDLGV+HPDGYIELKDRSKDIIISGGENISTIEVESVLF+HPSVL+AA+VGRPDDHWG
Sbjct: 425 RSGDLGVRHPDGYIELKDRSKDIIISGGENISTIEVESVLFNHPSVLEAAIVGRPDDHWG 484

Query: 486 ETPCAFVKLKDGCSATEGEIIKFCREHLPHYMAPRSVVFR-DLPKTSTGKTQKFILKKEA 545
           ETPCAFV LK G +ATE EIIKFCR++LPHYMAP++VVF+ DLPK+STGK QK ILK+EA
Sbjct: 485 ETPCAFVNLKHGSNATEEEIIKFCRDNLPHYMAPKTVVFKSDLPKSSTGKIQKLILKEEA 544

Query: 546 KAMGSLPKRVSKL 556
           KAMG+L    ++L
Sbjct: 545 KAMGTLSLAANRL 557

BLAST of CSPI04G26850 vs. NCBI nr
Match: gi|225457528|ref|XP_002270022.1| (PREDICTED: probable acyl-activating enzyme 1, peroxisomal isoform X1 [Vitis vinifera])

HSP 1 Score: 874.8 bits (2259), Expect = 8.1e-251
Identity = 412/554 (74.37%), Postives = 481/554 (86.82%), Query Frame = 1

Query: 1   MDGSHRCSANYVPLTPISFLERSAAVYGDRISLVYGRVQYTWRDTLQRCTRLASALVRTG 60
           M+G  RCSAN+VPL+PISFLERSA+VY DR+++VYGRV++TWRDTL+RCTRLASA+   G
Sbjct: 10  MEGMIRCSANHVPLSPISFLERSASVYRDRVAIVYGRVKFTWRDTLERCTRLASAITHLG 69

Query: 61  IARGDVVAALVPNIPAMYELHFAVPMAGAVLCSLNTRHDAAMVSTLLSHSEAKIIVVDYQ 120
           I+RGDVVAAL PNIPAMYELHF VPMAGAVLC+LN RHD+AMVSTLL HSEAK+I VDYQ
Sbjct: 70  ISRGDVVAALAPNIPAMYELHFGVPMAGAVLCTLNIRHDSAMVSTLLKHSEAKMIFVDYQ 129

Query: 121 LEHIVTGAIKAMSERKEKLPRVVIIQEYDQPPSRIDRPGSALEY-LEFESFLASGKLNFE 180
           L     GA++ +S+ + KLPR+V+I E D+    I   G  +   LE+E+ +A GK +F+
Sbjct: 130 LLDTAQGALEILSKTRTKLPRLVLIPECDKLQPTISNSGPTVAGDLEYETLIAMGKTDFD 189

Query: 181 IRRPRDELDPIALNYTSGTTSRPKGVIFSHRGAYLNSLSAVLLNDMCSLPVYLWTVPMFH 240
           IR P DE DPIALNYTSGTTSRPKGVI+SHRGA+LNSL+AVLLN+M S+PVYLWTVPMFH
Sbjct: 190 IRWPNDEWDPIALNYTSGTTSRPKGVIYSHRGAHLNSLAAVLLNEMGSMPVYLWTVPMFH 249

Query: 241 CNGWCLTWGVAAQSGTNICQRNVTAKEIFDNISLHKVTHMGGAPTVSNMIINAPISEQKP 300
           CNGWCLTWGVAAQ GTN+C RNVTAK IFD+IS H+VTHMGGAPTV NMIINAP+ +Q+P
Sbjct: 250 CNGWCLTWGVAAQGGTNVCLRNVTAKGIFDSISQHRVTHMGGAPTVLNMIINAPVGDQRP 309

Query: 301 LPREVTMMSGGAPPPSHVLYKLKALGFRIVHSYGLTETYGPATVCSWKPEWDSLPQDKQA 360
           LP +V +M+GGAPPP  VL+ +K LGF + HSYGLTETYGP TVC+WKPEWD LP DKQA
Sbjct: 310 LPGKVAVMTGGAPPPPQVLFNMKELGFGVFHSYGLTETYGPGTVCTWKPEWDLLPPDKQA 369

Query: 361 KLNSRQGLQHIGLEAADVKDPVTMESVPADGKTMGEVMLRGNTVMSGYLQDLKATREAFN 420
           K+N+RQGL H+G+E  D+KDPVTM+SVP D KT+GEVM RGNTVM+GYL+D+KAT E+F 
Sbjct: 370 KINARQGLHHLGMEEVDIKDPVTMKSVPPDAKTIGEVMFRGNTVMNGYLKDIKATEESFK 429

Query: 421 GGWFRSGDLGVKHPDGYIELKDRSKDIIISGGENISTIEVESVLFSHPSVLDAAVVGRPD 480
           GGWFRSGDLGVKHPDGYIELKDRSKDIIISGGENISTIEVE+VLFSHP++L+AAVVGRPD
Sbjct: 430 GGWFRSGDLGVKHPDGYIELKDRSKDIIISGGENISTIEVEAVLFSHPAILEAAVVGRPD 489

Query: 481 DHWGETPCAFVKLKDGCSATEGEIIKFCREHLPHYMAPRSVVFRDLPKTSTGKTQKFILK 540
           DHWGETPCAFVKLK+GC+A   EIIKFCR  LPHYMAP++VVF DLPKTSTGKTQK+ILK
Sbjct: 490 DHWGETPCAFVKLKEGCNANANEIIKFCRNKLPHYMAPKTVVFYDLPKTSTGKTQKYILK 549

Query: 541 KEAKAMGSLPKRVS 554
           ++AKAMGSL KR S
Sbjct: 550 EKAKAMGSLSKRSS 563

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AAE1_ARATH1.0e-22466.24Probable acyl-activating enzyme 1, peroxisomal OS=Arabidopsis thaliana GN=AAE1 P... [more]
AAE2_ARATH1.7e-20863.31Probable acyl-activating enzyme 2 OS=Arabidopsis thaliana GN=AAE2 PE=2 SV=1[more]
AEE7_ARATH3.5e-16952.42Acetate/butyrate--CoA ligase AAE7, peroxisomal OS=Arabidopsis thaliana GN=AAE7 P... [more]
AEE21_ARATH2.8e-16652.35Probable acyl-activating enzyme 21 OS=Arabidopsis thaliana GN=AEE21 PE=3 SV=1[more]
AAE11_ARATH3.5e-16149.10Butyrate--CoA ligase AAE11, peroxisomal OS=Arabidopsis thaliana GN=AAE11 PE=1 SV... [more]
Match NameE-valueIdentityDescription
A0A0A0L1B6_CUCSA0.0e+0099.82Uncharacterized protein OS=Cucumis sativus GN=Csa_4G658530 PE=4 SV=1[more]
A0A0A0L3B4_CUCSA7.9e-25376.67Uncharacterized protein OS=Cucumis sativus GN=Csa_4G658540 PE=4 SV=1[more]
F6I6S6_VITVI5.7e-25174.37Putative uncharacterized protein OS=Vitis vinifera GN=VIT_18s0122g01440 PE=4 SV=... [more]
M5VIW5_PRUPE1.1e-24673.92Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa003716mg PE=4 SV=1[more]
B9H5H2_POPTR3.0e-24472.55AMP-dependent synthetase and ligase family protein OS=Populus trichocarpa GN=POP... [more]
Match NameE-valueIdentityDescription
AT1G20560.15.6e-22666.24 acyl activating enzyme 1[more]
AT2G17650.19.7e-21063.31 AMP-dependent synthetase and ligase family protein[more]
AT3G16910.12.0e-17052.42 acyl-activating enzyme 7[more]
AT1G76290.11.6e-16752.35 AMP-dependent synthetase and ligase family protein[more]
AT1G66120.12.0e-16249.10 AMP-dependent synthetase and ligase family protein[more]
Match NameE-valueIdentityDescription
gi|449455591|ref|XP_004145536.1|0.0e+0099.82PREDICTED: probable acyl-activating enzyme 1, peroxisomal [Cucumis sativus][more]
gi|659104645|ref|XP_008452947.1|0.0e+0095.50PREDICTED: probable acyl-activating enzyme 1, peroxisomal [Cucumis melo][more]
gi|659104647|ref|XP_008452948.1|2.7e-25477.13PREDICTED: probable acyl-activating enzyme 1, peroxisomal [Cucumis melo][more]
gi|449455718|ref|XP_004145598.1|1.1e-25276.67PREDICTED: probable acyl-activating enzyme 1, peroxisomal [Cucumis sativus][more]
gi|225457528|ref|XP_002270022.1|8.1e-25174.37PREDICTED: probable acyl-activating enzyme 1, peroxisomal isoform X1 [Vitis vini... [more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000873AMP-dep_Synth/Lig
IPR020845AMP-binding_CS
IPR025110AMP-bd_C
Vocabulary: Molecular Function
TermDefinition
GO:0003824catalytic activity
Vocabulary: Biological Process
TermDefinition
GO:0008152metabolic process
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015996 chlorophyll catabolic process
biological_process GO:0006744 ubiquinone biosynthetic process
biological_process GO:0008152 metabolic process
cellular_component GO:0005777 peroxisome
cellular_component GO:0005575 cellular_component
molecular_function GO:0008756 o-succinylbenzoate-CoA ligase activity
molecular_function GO:0003824 catalytic activity
molecular_function GO:0016874 ligase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI04G26850.1CSPI04G26850.1mRNA


Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000873AMP-dependent synthetase/ligasePFAMPF00501AMP-bindingcoord: 20..449
score: 9.0
IPR020845AMP-binding, conserved sitePROSITEPS00455AMP_BINDINGcoord: 192..203
scor
IPR025110AMP-binding enzyme C-terminal domainPFAMPF13193AMP-binding_Ccoord: 458..532
score: 3.3
NoneNo IPR availableGENE3DG3DSA:2.30.38.10coord: 377..442
score: 1.2
NoneNo IPR availableGENE3DG3DSA:3.30.300.30coord: 446..544
score: 5.9
NoneNo IPR availableGENE3DG3DSA:3.40.50.980coord: 201..349
score: 7.4E-29coord: 14..127
score: 2.8E-32coord: 177..200
score: 2.8
NoneNo IPR availablePANTHERPTHR24095FAMILY NOT NAMEDcoord: 6..555
score:
NoneNo IPR availablePANTHERPTHR24095:SF119ACYL-ACTIVATING ENZYME 1, PEROXISOMAL-RELATEDcoord: 6..555
score:
NoneNo IPR availableunknownSSF56801Acetyl-CoA synthetase-likecoord: 8..544
score: 8.63E

The following gene(s) are paralogous to this gene:

None