CSPI04G22320 (gene) Wild cucumber (PI 183967)

BLAST of CSPI04G22320 vs. Swiss-Prot
Match: C7A29_PANGI (Cytochrome P450 CYP72A219 OS=Panax ginseng PE=2 SV=1)

HSP 1 Score: 640.6 bits (1651), Expect = 1.5e-182
Identity = 303/505 (60.00%), Postives = 388/505 (76.83%), Query Frame = 1

Query: 13  LVLLLLLGWKLADWIWFRPKKLEKLLRQQGFAGNSYRILHGDLKERAAMREEAMSKPMNF 72
           +V+++LLGW++ +W+W RP+KLEK LR QGF GNSYR+  GD+KE   M +EA SKP+N 
Sbjct: 14  IVVVILLGWRIFNWVWLRPRKLEKYLRNQGFNGNSYRLFFGDVKEMIVMLKEAKSKPINL 73

Query: 73  SNHIAPRVIPSIYHTIQRYGKNSFMWLGPIPRVHIMDPEQLKTVFSFFNDFQKPTMNPFT 132
            + I PR+IP     I  YGKNSF+WLGP P VHIM+P+ +K V S F  FQKP  NP T
Sbjct: 74  YDDIIPRIIPLNQKIITNYGKNSFLWLGPKPMVHIMNPDHIKDVLSKFYQFQKPRHNPLT 133

Query: 133 KLLVQGIVNLDGEKWVKHRKIINPAFHLEKLKDMVPAFYHSCNEMVSKWESMVSKEGSCE 192
           KLL  G+ + +G++W KHRK+INPAFHLEKLK+M+PA Y S +E+V+KWE MVS +G  E
Sbjct: 134 KLLATGVADAEGDRWAKHRKLINPAFHLEKLKNMLPAIYLSSSEIVTKWEEMVSTKGQFE 193

Query: 193 LDVMPYLKNMASDVISRTAFGSSYEKGKKIFKLQTELTNFVIQTTLGIYIPGWRFLPTKL 252
           LDV+PYL+ + SDVISRTAFGSSYE+G+KIF+LQ E    +IQ +  IY+PG RFLPTK 
Sbjct: 194 LDVLPYLETLTSDVISRTAFGSSYEEGRKIFQLQREQAELIIQASQTIYLPGMRFLPTKR 253

Query: 253 NRKMKEISKEITTLILGIMDEREKSMKAGE-AIQTDLLSILMESNMNEIKQHGNKKDIGM 312
           N++MKEI+KE+   +  I+++R K+M+AGE +   DLL IL+ESN  EIKQHGN  + G+
Sbjct: 254 NKRMKEIAKEVKIALKSIINKRLKAMEAGERSSHDDLLGILLESNSKEIKQHGN-TNFGL 313

Query: 313 SIEDVIEECKLFYIAGQETTATLLVWTMILLSSYSEWQERARAEVFEIFGNKQPNYDGLN 372
           ++++VIEECKLF+ AGQETT+ LLVWTMILLS + +WQ+RA+ EV   FGN +P++DGLN
Sbjct: 314 TVDEVIEECKLFFFAGQETTSNLLVWTMILLSQHQDWQKRAKEEVLRTFGNNKPDFDGLN 373

Query: 373 RLKVVTMIFNEVLRLYPPGSLFVRIVRKETRLGNLTLPGGVMLGLPIVLIQRDPELWGED 432
            LKVV MI  EVLRLYPP     R + +E +LG ++LP GV+L LPI+L+  D E+WG+D
Sbjct: 374 HLKVVNMILLEVLRLYPPILSLDRTIYEEIKLGEISLPAGVILLLPIILLHYDQEIWGDD 433

Query: 433 AHEFNPERFSGGVSKATKNPSAFIPFGWGPRICIGQTFAMIEAKMALSMILQRFSFELSP 492
           A EFNPERFS GV KATK    + PF WGPRICIGQ FAM+EAKMA++MILQRFSF LSP
Sbjct: 434 AKEFNPERFSEGVLKATKGRVTYFPFSWGPRICIGQNFAMLEAKMAMAMILQRFSFVLSP 493

Query: 493 SYTHSPIASLTTQPQHGAHIILHKL 517
           SY H+P A +T QPQ+GAH+ILH L
Sbjct: 494 SYAHAPHAIITLQPQYGAHLILHSL 517

BLAST of CSPI04G22320 vs. Swiss-Prot
Match: C7A15_ARATH (Cytochrome P450 72A15 OS=Arabidopsis thaliana GN=CYP72A15 PE=2 SV=1)

HSP 1 Score: 596.7 bits (1537), Expect = 2.5e-169
Identity = 281/509 (55.21%), Postives = 377/509 (74.07%), Query Frame = 1

Query: 9   ISIVLVLLLLLGWKLADWIWFRPKKLEKLLRQQGFAGNSYRILHGDLKERAAMREEAMSK 68
           IS+VL ++    W+   W+WF+PK LE  LR+QG AG  Y  L GDLK+   M  EA SK
Sbjct: 10  ISVVLAVVSWWIWRTLQWVWFKPKMLEHYLRRQGLAGTPYTPLVGDLKKNFTMLSEARSK 69

Query: 69  PMNFSNHIAPRVIPSIYHTIQRYGKNSFMWLGPIPRVHIMDPEQLKTVFSFFNDFQKPTM 128
           P+  ++ I+PRV+P      + YG+  F W GPIP + IMDPEQ+K VF+   DFQKP  
Sbjct: 70  PLKLTDDISPRVVPYPLQMFKTYGRTYFTWFGPIPTITIMDPEQIKEVFNKVYDFQKPHT 129

Query: 129 NPFTKLLVQGIVNLDGEKWVKHRKIINPAFHLEKLKDMVPAFYHSCNEMVSKWESMVSKE 188
            P   ++ +G+ N DG+KW KHR+IINPAFH+EK+K+MVPAF+ SC E+V +W+ +VS +
Sbjct: 130 FPLATIIAKGLANYDGDKWAKHRRIINPAFHIEKIKNMVPAFHQSCREVVGEWDQLVSDK 189

Query: 189 GS-CELDVMPYLKNMASDVISRTAFGSSYEKGKKIFKLQTELTNFVIQTTLGIYIPGWRF 248
           GS CE+DV P L +M +DVISRTAFGSSY++G++IF+LQ EL   +IQ     +IPG+ +
Sbjct: 190 GSSCEVDVWPGLVSMTADVISRTAFGSSYKEGQRIFELQAELAQLIIQAFRKAFIPGYSY 249

Query: 249 LPTKLNRKMKEISKEITTLILGIMDEREKSMKAGEAIQTDLLSILMESNMNEIKQHGNKK 308
           LPTK NR+MK  ++EI  ++ GI+++R ++ +AGEA   DLL IL+ESN+ + +  GN  
Sbjct: 250 LPTKSNRRMKAAAREIQVILRGIVNKRLRAREAGEAPSDDLLGILLESNLRQTE--GN-- 309

Query: 309 DIGMSIEDVIEECKLFYIAGQETTATLLVWTMILLSSYSEWQERARAEVFEIFGNKQPNY 368
             GMS ED++EECKLFY AGQETT+ LLVWTM+LLS + +WQ RAR EV ++FG+K+P+ 
Sbjct: 310 --GMSTEDLMEECKLFYFAGQETTSVLLVWTMVLLSQHQDWQARAREEVKQVFGDKEPDA 369

Query: 369 DGLNRLKVVTMIFNEVLRLYPPGSLFVRIVRKETRLGNLTLPGGVMLGLPIVLIQRDPEL 428
           +GLN+LKV+TMI  EVLRLYPP +   R + KE +LG+LTLPGGV + LPI+L+Q D EL
Sbjct: 370 EGLNQLKVMTMILYEVLRLYPPVTQLTRAIHKELKLGDLTLPGGVQISLPILLVQHDIEL 429

Query: 429 WGEDAHEFNPERFSGGVSKATKNPSAFIPFGWGPRICIGQTFAMIEAKMALSMILQRFSF 488
           WG DA EFNP+RF  G+SKATK+  +F PF WGPRICIGQ FA++EAKMA+++IL+RFSF
Sbjct: 430 WGNDAAEFNPDRFKDGLSKATKSQVSFFPFAWGPRICIGQNFALLEAKMAMALILRRFSF 489

Query: 489 ELSPSYTHSPIASLTTQPQHGAHIILHKL 517
           E+SPSY H+P   +T  PQ GA +I+HKL
Sbjct: 490 EISPSYVHAPYTVITIHPQFGAQLIMHKL 512

BLAST of CSPI04G22320 vs. Swiss-Prot
Match: C72A1_CATRO (Secologanin synthase OS=Catharanthus roseus GN=CYP72A1 PE=2 SV=1)

HSP 1 Score: 594.0 bits (1530), Expect = 1.6e-168
Identity = 275/511 (53.82%), Postives = 369/511 (72.21%), Query Frame = 1

Query: 8   IISIVLVLLLLLGWKLADWIWFRPKKLEKLLRQQGFAGNSYRILHGDLKERAAMREEAMS 67
           I + +  L++   W++ DW WF PK++EK LRQQGF GN YR L GD+KE   M +EA+S
Sbjct: 12  IAATIFALVMAWAWRVLDWAWFTPKRIEKRLRQQGFRGNPYRFLVGDVKESGKMHQEALS 71

Query: 68  KPMNFSNHIAPRVIPSIYHTIQRYGKNSFMWLGPIPRVHIMDPEQLKTVFSFFNDFQK-- 127
           KPM F+N I PR++P I HTI  YG+NSF W+G IPR+H+M+PE +K V +  + +QK  
Sbjct: 72  KPMEFNNDIVPRLMPHINHTINTYGRNSFTWMGRIPRIHVMEPELIKEVLTHSSKYQKNF 131

Query: 128 PTMNPFTKLLVQGIVNLDGEKWVKHRKIINPAFHLEKLKDMVPAFYHSCNEMVSKWESMV 187
              NP  K L+ G+ + +G KW KHR+II+PAF LEKLK M+PAF    ++M++KWE + 
Sbjct: 132 DVHNPLVKFLLTGVGSFEGAKWSKHRRIISPAFTLEKLKSMLPAFAICYHDMLTKWEKIA 191

Query: 188 SKEGSCELDVMPYLKNMASDVISRTAFGSSYEKGKKIFKLQTELTNFVIQTTLGIYIPGW 247
            K+GS E+D+ P    + SDVIS+ AFGS+YE+G KIF+L  EL +  I     +YIPGW
Sbjct: 192 EKQGSHEVDIFPTFDVLTSDVISKVAFGSTYEEGGKIFRLLKELMDLTIDCMRDVYIPGW 251

Query: 248 RFLPTKLNRKMKEISKEITTLILGIMDEREKSMKAGEAIQTDLLSILMESNMNEIKQHGN 307
            +LPTK N++MKEI+KEIT ++  I+++R K++KAGE  + DLL +L+ESN+ EI++ GN
Sbjct: 252 SYLPTKRNKRMKEINKEITDMLRFIINKRMKALKAGEPGEDDLLGVLLESNIQEIQKQGN 311

Query: 308 KKDIGMSIEDVIEECKLFYIAGQETTATLLVWTMILLSSYSEWQERARAEVFEIFGNKQP 367
           KKD GMSI DVIEECKLFY AGQETT  LL WT ILLS + EWQERAR EV + FG  +P
Sbjct: 312 KKDGGMSINDVIEECKLFYFAGQETTGVLLTWTTILLSKHPEWQERAREEVLQAFGKNKP 371

Query: 368 NYDGLNRLKVVTMIFNEVLRLYPPGSLFVRIVRKETRLGNLTLPGGVMLGLPIVLIQRDP 427
            ++ LN LK V+MI  EVLRLYPP     +IV K+T+LG+ T+P G  + LP V++ R+ 
Sbjct: 372 EFERLNHLKYVSMILYEVLRLYPPVIDLTKIVHKDTKLGSYTIPAGTQVMLPTVMLHREK 431

Query: 428 ELWGEDAHEFNPERFSGGVSKATKNPSAFIPFGWGPRICIGQTFAMIEAKMALSMILQRF 487
            +WGEDA EFNP RF  GV+ ATKN   ++PF WGPR+C+GQ FA+++AK+ L+MILQRF
Sbjct: 432 SIWGEDAMEFNPMRFVDGVANATKNNVTYLPFSWGPRVCLGQNFALLQAKLGLAMILQRF 491

Query: 488 SFELSPSYTHSPIASLTTQPQHGAHIILHKL 517
            F+++PSY H+P   LT QPQ G+H+I  KL
Sbjct: 492 KFDVAPSYVHAPFTILTVQPQFGSHVIYKKL 522

BLAST of CSPI04G22320 vs. Swiss-Prot
Match: C7A13_ARATH (Cytochrome P450 72A13 OS=Arabidopsis thaliana GN=CYP72A13 PE=2 SV=1)

HSP 1 Score: 587.4 bits (1513), Expect = 1.5e-166
Identity = 280/509 (55.01%), Postives = 376/509 (73.87%), Query Frame = 1

Query: 9   ISIVLVLLLLLGWKLADWIWFRPKKLEKLLRQQGFAGNSYRILHGDLKERAAMREEAMSK 68
           +S+ +V++    W+    +W +PK LE  LR+QG AG  Y  L GDLK   +M  EA SK
Sbjct: 10  VSVAVVVVSWWVWRTLQRVWLKPKMLESYLRRQGLAGTPYTPLVGDLKRNFSMLAEARSK 69

Query: 69  PMNFSNHIAPRVIPSIYHTIQRYGKNSFMWLGPIPRVHIMDPEQLKTVFSFFNDFQKPTM 128
           P+N ++ I PR++P     ++ +G+  F W GPIP + IMDPEQ+K VF+   DFQK   
Sbjct: 70  PINLTDDITPRIVPYPLQMLKTHGRTFFTWFGPIPTITIMDPEQIKEVFNKVYDFQKAHT 129

Query: 129 NPFTKLLVQGIVNLDGEKWVKHRKIINPAFHLEKLKDMVPAFYHSCNEMVSKWESMVS-K 188
            P  +L+  G+V+ DG+KW KHR+IINPAFHLEK+K+MVPAF+ SC+E+V +W+ +V+ K
Sbjct: 130 FPLGRLIAAGLVSYDGDKWTKHRRIINPAFHLEKIKNMVPAFHQSCSEIVGEWDKLVTDK 189

Query: 189 EGSCELDVMPYLKNMASDVISRTAFGSSYEKGKKIFKLQTELTNFVIQTTLGIYIPGWRF 248
           + SCE+D+ P+L +M +DVISRTAFGSSY++G++IF+LQ EL   +IQ      IPG+R+
Sbjct: 190 QSSCEVDIWPWLVSMTADVISRTAFGSSYKEGQRIFELQAELAQLIIQAFRKAIIPGYRY 249

Query: 249 LPTKLNRKMKEISKEITTLILGIMDEREKSMKAGEAIQTDLLSILMESNMNEIKQHGNKK 308
            PTK NR+MK  ++EI  ++ GI+++R ++ +AGEA   DLL IL+ESN+ + K  GN  
Sbjct: 250 FPTKGNRRMKAAAREIKFILRGIVNKRLRAREAGEAPSDDLLGILLESNLGQTK--GN-- 309

Query: 309 DIGMSIEDVIEECKLFYIAGQETTATLLVWTMILLSSYSEWQERARAEVFEIFGNKQPNY 368
             GMS E+++EECKLFY AGQETT  LLVWTM+LLS + +WQ RAR EV ++FG+K+P+ 
Sbjct: 310 --GMSTEELMEECKLFYFAGQETTTVLLVWTMVLLSQHQDWQARAREEVKQVFGDKEPDA 369

Query: 369 DGLNRLKVVTMIFNEVLRLYPPGSLFVRIVRKETRLGNLTLPGGVMLGLPIVLIQRDPEL 428
           +GLN+LKV+TMI  EVLRLYPP     R + KE +LG+LTLPGGV + LPI+LIQRD EL
Sbjct: 370 EGLNQLKVMTMILYEVLRLYPPVVQLTRAIHKEMQLGDLTLPGGVQISLPILLIQRDREL 429

Query: 429 WGEDAHEFNPERFSGGVSKATKNPSAFIPFGWGPRICIGQTFAMIEAKMALSMILQRFSF 488
           WG DA EF P+RF  G+SKATKN  +F PF WGPRICIGQ FA++EAKMA+++IL++FSF
Sbjct: 430 WGNDAGEFKPDRFKDGLSKATKNQVSFFPFAWGPRICIGQNFALLEAKMAMTLILRKFSF 489

Query: 489 ELSPSYTHSPIASLTTQPQHGAHIILHKL 517
           ELSPSY H+P   LTT PQ GA +ILHKL
Sbjct: 490 ELSPSYVHAPYTVLTTHPQFGAPLILHKL 512

BLAST of CSPI04G22320 vs. Swiss-Prot
Match: C7A11_ARATH (Cytochrome P450 72A11 OS=Arabidopsis thaliana GN=CYP72A11 PE=2 SV=1)

HSP 1 Score: 586.6 bits (1511), Expect = 2.6e-166
Identity = 280/509 (55.01%), Postives = 374/509 (73.48%), Query Frame = 1

Query: 9   ISIVLVLLLLLGWKLADWIWFRPKKLEKLLRQQGFAGNSYRILHGDLKERAAMREEAMSK 68
           +S+ +V++    W+   W+WF+PK LE  LR+QG AG  Y  L GDLK+  +MR EA SK
Sbjct: 10  VSVAVVVVSWWVWRTLQWVWFKPKMLESYLRRQGLAGTPYTPLVGDLKKNFSMRAEARSK 69

Query: 69  PMNFSNHIAPRVIPSIYHTIQRYGKNSFMWLGPIPRVHIMDPEQLKTVFSFFNDFQKPTM 128
           P+N ++ I PR++P     ++ +G+  F W G IP + IMDPEQ+  V +   DFQK   
Sbjct: 70  PINLTDDITPRIVPYPLQMLKTHGRTFFTWFGAIPTITIMDPEQITEVLNKVYDFQKAHT 129

Query: 129 NPFTKLLVQGIVNLDGEKWVKHRKIINPAFHLEKLKDMVPAFYHSCNEMVSKWESMVS-K 188
            P  +L+  G+++ DG+KW KHR+IINPAFHLEK+K+MVPAF+ SC+E+V KW+ +VS K
Sbjct: 130 FPLGRLIATGVLSYDGDKWAKHRRIINPAFHLEKIKNMVPAFHQSCSEIVCKWDKLVSDK 189

Query: 189 EGSCELDVMPYLKNMASDVISRTAFGSSYEKGKKIFKLQTELTNFVIQTTLGIYIPGWRF 248
           E SCE+DV P L +M +DVISRTAFGSS  +G++IF+LQ EL   +IQT    +IPG+ +
Sbjct: 190 ESSCEVDVWPGLVSMTADVISRTAFGSSCVEGQRIFELQAELAQLIIQTVRKAFIPGYSY 249

Query: 249 LPTKLNRKMKEISKEITTLILGIMDEREKSMKAGEAIQTDLLSILMESNMNEIKQHGNKK 308
           LPTK NR+MK  ++EI  ++ GI+++R ++ +AGEA   DLL IL+ESN+ + K  GN  
Sbjct: 250 LPTKGNRRMKAKAREIQVILRGIVNKRLRAREAGEAPNDDLLGILLESNLGQTK--GN-- 309

Query: 309 DIGMSIEDVIEECKLFYIAGQETTATLLVWTMILLSSYSEWQERARAEVFEIFGNKQPNY 368
             GMS ED++EECKLFY  GQETT+ LLVWTM+LLS + +WQ RAR EV ++FG+K+P+ 
Sbjct: 310 --GMSTEDLMEECKLFYFVGQETTSVLLVWTMVLLSQHQDWQARAREEVKQVFGDKEPDA 369

Query: 369 DGLNRLKVVTMIFNEVLRLYPPGSLFVRIVRKETRLGNLTLPGGVMLGLPIVLIQRDPEL 428
           +GLN+LKV+TMI  EVLRLYPP     R + KE  LG+LTLPGGV++ LPI+L+QRD EL
Sbjct: 370 EGLNQLKVMTMILYEVLRLYPPIPQLSRAIHKEMELGDLTLPGGVLINLPILLVQRDTEL 429

Query: 429 WGEDAHEFNPERFSGGVSKATKNPSAFIPFGWGPRICIGQTFAMIEAKMALSMILQRFSF 488
           WG DA EF P+RF  G+SKATKN ++F PF WG RICIGQ FA++EAKMA+++ILQRFSF
Sbjct: 430 WGNDAGEFKPDRFKDGLSKATKNQASFFPFAWGSRICIGQNFALLEAKMAMALILQRFSF 489

Query: 489 ELSPSYTHSPIASLTTQPQHGAHIILHKL 517
           ELSPSY H+P    T  PQ GA +I+HKL
Sbjct: 490 ELSPSYVHAPYTVFTIHPQFGAPLIMHKL 512

BLAST of CSPI04G22320 vs. TrEMBL
Match: V7BTM1_PHAVU (Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_005G063300g PE=3 SV=1)

HSP 1 Score: 674.9 bits (1740), Expect = 8.0e-191
Identity = 309/512 (60.35%), Postives = 405/512 (79.10%), Query Frame = 1

Query: 7   TIISIVLVLLLLLGWKLADWIWFRPKKLEKLLRQQGFAGNSYRILHGDLKERAAMREEAM 66
           T ++++++LLL   WK+ +W+WF PK+LEKLLR+QGF GN Y++L GD KE   MR+EA+
Sbjct: 7   TTLTLIVILLLTWAWKILNWLWFNPKRLEKLLREQGFQGNPYKLLVGDSKEFLKMRKEAL 66

Query: 67  SKPMNFSNHIAPRVIPSIYHTIQRYGKNSFMWLGPIPRVHIMDPEQLKTVFSFFNDFQKP 126
           SKPM+ S+ I PRV   I H+++ +GKNSF+WLGP PRV I+DPEQ+K +F+   DF KP
Sbjct: 67  SKPMDLSDDIVPRVSSYINHSVKSHGKNSFIWLGPTPRVTILDPEQIKDIFNKIYDFPKP 126

Query: 127 TMNPFTKLLVQGIVNLDGEKWVKHRKIINPAFHLEKLKDMVPAFYHSCNEMVSKWESMVS 186
            +NP  KLL  G+   +GEKW KHR++INPAF+LEKLK M+P F+ SCN++V+KWE M+S
Sbjct: 127 DINPLFKLLATGLAGYEGEKWSKHRRLINPAFNLEKLKAMLPLFFESCNDLVNKWERMLS 186

Query: 187 KEGSCELDVMPYLKNMASDVISRTAFGSSYEKGKKIFKLQTELTNFVIQTTLGIYIPGWR 246
            +GSCE+D  P+L+NM SDVI+R+AFGSS+E+G++IF+LQ E T   +Q  L + IPGWR
Sbjct: 187 SDGSCEIDAWPFLQNMTSDVIARSAFGSSFEEGRRIFQLQREQTKLALQVILKVQIPGWR 246

Query: 247 FLPTKLNRKMKEISKEITTLILGIMDEREKSMKAGEAIQTDLLSILMESNMNEIKQH--G 306
           FLPTK +R+MKEI ++I   +  ++ +REK++K GEAI  DLL IL+ESN  EI++H  G
Sbjct: 247 FLPTKSHRRMKEIDRDIKASLKDMIYKREKALKKGEAINDDLLGILLESNHKEIQEHAKG 306

Query: 307 NKKDIGMSIEDVIEECKLFYIAGQETTATLLVWTMILLSSYSEWQERARAEVFEIFGNKQ 366
           + K++GMS+EDVIEECKLFY AGQETT+ LLVWTM+LLS Y +WQERAR EV ++FGN++
Sbjct: 307 DNKNVGMSLEDVIEECKLFYFAGQETTSVLLVWTMVLLSRYPDWQERAREEVLQVFGNQK 366

Query: 367 PNYDGLNRLKVVTMIFNEVLRLYPPGSLFVRIVRKETRLGNLTLPGGVMLGLPIVLIQRD 426
           P++DGLNRLK+VTMI NEVLRLYPP     R+ +++ +LGN+TLP GV + LP VL+  D
Sbjct: 367 PSFDGLNRLKIVTMILNEVLRLYPPALGMNRLAKRDLKLGNITLPAGVQIFLPTVLVHHD 426

Query: 427 PELWGEDAHEFNPERFSGGVSKATKNPSAFIPFGWGPRICIGQTFAMIEAKMALSMILQR 486
            E WGEDA +FNPERFS GV KATK   +F PFGWGPRICIGQ F+++EAKMALSMILQ 
Sbjct: 427 CEFWGEDAKQFNPERFSEGVLKATKGRVSFFPFGWGPRICIGQNFSLLEAKMALSMILQH 486

Query: 487 FSFELSPSYTHSPIASLTTQPQHGAHIILHKL 517
           FSFELSP+Y H+P   +T QPQ+GAHIILHK+
Sbjct: 487 FSFELSPAYAHAPAMLITLQPQYGAHIILHKV 518

BLAST of CSPI04G22320 vs. TrEMBL
Match: W9QZH6_9ROSA (Secologanin synthase OS=Morus notabilis GN=L484_024637 PE=3 SV=1)

HSP 1 Score: 673.7 bits (1737), Expect = 1.8e-190
Identity = 313/508 (61.61%), Postives = 394/508 (77.56%), Query Frame = 1

Query: 10  SIVLVLLLLL--GWKLADWIWFRPKKLEKLLRQQGFAGNSYRILHGDLKERAAMREEAMS 69
           S+V  +LL+    W++ +W+W RPK+LE  LR+QG  GNSYR+  GDLKE + M +EA S
Sbjct: 10  SVVFAILLITTWAWRVLNWVWLRPKRLENCLRKQGLKGNSYRLWSGDLKESSLMIKEASS 69

Query: 70  KPMNFSNHIAPRVIPSIYHTIQRYGKNSFMWLGPIPRVHIMDPEQLKTVFSFFNDFQKPT 129
           KP+N S  I  R+ P   HT+  YGK+SFMW+GP PRV I +PE +K +F+   DF KP 
Sbjct: 70  KPINLSEDITARIFPFGLHTVNNYGKDSFMWIGPTPRVSITNPEYIKDIFNKLGDFPKPQ 129

Query: 130 MNPFTKLLVQGIVNLDGEKWVKHRKIINPAFHLEKLKDMVPAFYHSCNEMVSKWESMVSK 189
             P  KLL  G+   +GEKW +HRKIINPAFH+EKLK +VPAFY SC+EM++KWE +++ 
Sbjct: 130 SIPLVKLLATGVATYEGEKWTRHRKIINPAFHMEKLKRLVPAFYLSCSEMLNKWERLLNS 189

Query: 190 EGSCELDVMPYLKNMASDVISRTAFGSSYEKGKKIFKLQTELTNFVIQTTLGIYIPGWRF 249
           EGSCELDV  YL+NM  DVISRTAFGSSYE+G++IF+LQ E     I++   +YIPGWRF
Sbjct: 190 EGSCELDVWSYLQNMTGDVISRTAFGSSYEEGRRIFQLQKEQGELAIKSLQTVYIPGWRF 249

Query: 250 LPTKLNRKMKEISKEITTLILGIMDEREKSMKAGEAIQTDLLSILMESNMNEIKQHGNKK 309
           +PTKLNR+MK+I  EI  L+  I+ +RE +M+AGEA   DLL ILMESN+ EI++HGN K
Sbjct: 250 VPTKLNRRMKKIYNEIEALVKNIIHKREGAMRAGEAPNDDLLGILMESNLKEIQEHGNNK 309

Query: 310 DIGMSIEDVIEECKLFYIAGQETTATLLVWTMILLSSYSEWQERARAEVFEIFGNKQPNY 369
           ++G+SI DVIEECKLFYIAGQETT+TLLVWTM+LLS +  WQ+RAR EV +IFGN +P +
Sbjct: 310 NMGISIRDVIEECKLFYIAGQETTSTLLVWTMVLLSRFPSWQDRAREEVLQIFGNNKPGF 369

Query: 370 DGLNRLKVVTMIFNEVLRLYPPGSLFVRIVRKETRLGNLTLPGGVMLGLPIVLIQRDPEL 429
           DGL +LKVVTMIF EVLRLYPP  LF R V K+T+LGNL+LP GV + +P +L+ RD EL
Sbjct: 370 DGLTQLKVVTMIFYEVLRLYPPLILFTRKVEKKTQLGNLSLPAGVEVSIPTILVHRDTEL 429

Query: 430 WGEDAHEFNPERFSGGVSKATKNPSAFIPFGWGPRICIGQTFAMIEAKMALSMILQRFSF 489
           WGEDA +F PERF+ G+SK TK   +F PFGWGPRIC+GQ FAM+EAKMAL++ILQRF+F
Sbjct: 430 WGEDAKDFKPERFAEGMSKVTKGQVSFFPFGWGPRICVGQNFAMVEAKMALALILQRFTF 489

Query: 490 ELSPSYTHSPIASLTTQPQHGAHIILHK 516
           ELSPSY H+P+  LT QPQ+GAHIILHK
Sbjct: 490 ELSPSYAHAPLTILTLQPQYGAHIILHK 517

BLAST of CSPI04G22320 vs. TrEMBL
Match: A0A0B2SBC8_GLYSO (Secologanin synthase OS=Glycine soja GN=glysoja_039974 PE=3 SV=1)

HSP 1 Score: 673.3 bits (1736), Expect = 2.3e-190
Identity = 313/515 (60.78%), Postives = 403/515 (78.25%), Query Frame = 1

Query: 4   APDTIISIVLVLLLLLGWKLADWIWFRPKKLEKLLRQQGFAGNSYRILHGDLKERAAMRE 63
           A  TI+ ++L+L L+  WK+ +W+W RPK+LEKLLR+QGF GN Y +  GD KE   MR+
Sbjct: 4   ASATILLLILILALICSWKVLNWLWLRPKRLEKLLREQGFRGNPYTLFFGDSKEFLKMRK 63

Query: 64  EAMSKPMNFSNHIAPRVIPSIYHTIQRYGKNSFMWLGPIPRVHIMDPEQLKTVFSFFNDF 123
           EA+SKPM  S+ I PRV   + H++ ++GKNSF+W GP+PRV I+DPEQ+K VF+   DF
Sbjct: 64  EAVSKPMTLSDDIVPRVSAYVQHSVNKHGKNSFIWFGPMPRVTILDPEQIKDVFNKNYDF 123

Query: 124 QKPTMNPFTKLLVQGIVNLDGEKWVKHRKIINPAFHLEKLKDMVPAFYHSCNEMVSKWES 183
            KP +NP  KLL  G+   +GEKW KHR+IINPAF+LEKLK M+P F  SCN++VSKWE 
Sbjct: 124 PKPNLNPLVKLLATGLAGYEGEKWSKHRRIINPAFNLEKLKIMLPLFLQSCNDLVSKWEG 183

Query: 184 MVSKEGSCELDVMPYLKNMASDVISRTAFGSSYEKGKKIFKLQTELTNFVIQTTLGIYIP 243
           M+S EGSCE+D  P+L+N+ SDVI+R+AFGSSYE+G++IF+LQ E T  +++  L I IP
Sbjct: 184 MLSSEGSCEMDAWPFLQNLTSDVIARSAFGSSYEEGRRIFQLQREQTELLMKVILKIQIP 243

Query: 244 GWRFLPTKLNRKMKEISKEITTLILGIMDEREKSMKAGEAIQTDLLSILMESNMNEIKQH 303
           GWRFLPTK +R+MKEI +EI   +  ++++REK++K+GEA + DLL IL+ESN  EI++H
Sbjct: 244 GWRFLPTKTHRRMKEIDREIKASLKNMINKREKALKSGEATKNDLLGILLESNHKEIQEH 303

Query: 304 GNK--KDIGMSIEDVIEECKLFYIAGQETTATLLVWTMILLSSYSEWQERARAEVFEIFG 363
           GN+  K++GMS+EDVIEECKLFY AGQETT+ LLVWTM+LLS Y +WQ RAR E F++FG
Sbjct: 304 GNRNSKNVGMSLEDVIEECKLFYFAGQETTSVLLVWTMVLLSRYPDWQARAREEAFQVFG 363

Query: 364 NKQPNYDGLNRLKVVTMIFNEVLRLYPPGSLFVRIVRKETRLGNLTLPGGVMLGLPIVLI 423
            ++P++DGL+RLK+VTMI  EVLRLYPP     R+V K+ +LGNLTLP GV + LP VLI
Sbjct: 364 YQKPDFDGLSRLKIVTMILYEVLRLYPPLIGMNRLVEKDVKLGNLTLPAGVQVFLPTVLI 423

Query: 424 QRDPELWGEDAHEFNPERFSGGVSKATKNPSAFIPFGWGPRICIGQTFAMIEAKMALSMI 483
             D ELWGEDA +FNPERFS GV KAT    +F PFGWGPRICIGQ F+++EAKMALSMI
Sbjct: 424 HHDSELWGEDAKQFNPERFSEGVLKATNGRVSFFPFGWGPRICIGQNFSLLEAKMALSMI 483

Query: 484 LQRFSFELSPSYTHSPIASLTTQPQHGAHIILHKL 517
           LQ F FELSP+Y H+P  S+TTQPQ+GAHIIL K+
Sbjct: 484 LQNFLFELSPAYAHAPTMSITTQPQYGAHIILRKV 518

BLAST of CSPI04G22320 vs. TrEMBL
Match: I1MIY5_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_15G244300 PE=3 SV=1)

HSP 1 Score: 672.9 bits (1735), Expect = 3.0e-190
Identity = 313/515 (60.78%), Postives = 403/515 (78.25%), Query Frame = 1

Query: 4   APDTIISIVLVLLLLLGWKLADWIWFRPKKLEKLLRQQGFAGNSYRILHGDLKERAAMRE 63
           A  TI+ ++L+L L+  WK+ +W+W RPK+LEKLLR+QGF GN Y +  GD KE   MR+
Sbjct: 4   ASATILLLILILALICSWKVLNWLWLRPKRLEKLLREQGFRGNPYTLFFGDSKEFLKMRK 63

Query: 64  EAMSKPMNFSNHIAPRVIPSIYHTIQRYGKNSFMWLGPIPRVHIMDPEQLKTVFSFFNDF 123
           EA+SKPM  S+ I PRV   + H++ ++GKNSF+W GP+PRV I+DPEQ+K VF+   DF
Sbjct: 64  EAVSKPMTLSDDIVPRVSAYVQHSVNKHGKNSFIWFGPMPRVTILDPEQIKDVFNKNYDF 123

Query: 124 QKPTMNPFTKLLVQGIVNLDGEKWVKHRKIINPAFHLEKLKDMVPAFYHSCNEMVSKWES 183
            KP +NP  KLL  G+   +GEKW KHR+IINPAF+LEKLK M+P F  SCN++VSKWE 
Sbjct: 124 PKPNLNPLVKLLATGLAGYEGEKWSKHRRIINPAFNLEKLKIMLPLFLQSCNDLVSKWEG 183

Query: 184 MVSKEGSCELDVMPYLKNMASDVISRTAFGSSYEKGKKIFKLQTELTNFVIQTTLGIYIP 243
           M+S EGSCE+D  P+L+N+ SDVI+R+AFGSSYE+G++IF+LQ E T  +++  L I IP
Sbjct: 184 MLSSEGSCEMDAWPFLQNLTSDVIARSAFGSSYEEGRRIFQLQREQTEHLMKVILKIQIP 243

Query: 244 GWRFLPTKLNRKMKEISKEITTLILGIMDEREKSMKAGEAIQTDLLSILMESNMNEIKQH 303
           GWRFLPTK +R+MKEI +EI   +  ++++REK++K+GEA + DLL IL+ESN  EI++H
Sbjct: 244 GWRFLPTKTHRRMKEIDREIKASLKNMINKREKALKSGEATKNDLLGILLESNHKEIQEH 303

Query: 304 GNK--KDIGMSIEDVIEECKLFYIAGQETTATLLVWTMILLSSYSEWQERARAEVFEIFG 363
           GN+  K++GMS+EDVIEECKLFY AGQETT+ LLVWTM+LLS Y +WQ RAR E F++FG
Sbjct: 304 GNRNSKNVGMSLEDVIEECKLFYFAGQETTSVLLVWTMVLLSRYPDWQARAREEAFQVFG 363

Query: 364 NKQPNYDGLNRLKVVTMIFNEVLRLYPPGSLFVRIVRKETRLGNLTLPGGVMLGLPIVLI 423
            ++P++DGL+RLK+VTMI  EVLRLYPP     R+V K+ +LGNLTLP GV + LP VLI
Sbjct: 364 YQKPDFDGLSRLKIVTMILYEVLRLYPPLIGMNRLVEKDVKLGNLTLPAGVQVFLPTVLI 423

Query: 424 QRDPELWGEDAHEFNPERFSGGVSKATKNPSAFIPFGWGPRICIGQTFAMIEAKMALSMI 483
             D ELWGEDA +FNPERFS GV KAT    +F PFGWGPRICIGQ F+++EAKMALSMI
Sbjct: 424 HHDSELWGEDAKQFNPERFSEGVLKATNGRVSFFPFGWGPRICIGQNFSLLEAKMALSMI 483

Query: 484 LQRFSFELSPSYTHSPIASLTTQPQHGAHIILHKL 517
           LQ F FELSP+Y H+P  S+TTQPQ+GAHIIL K+
Sbjct: 484 LQNFLFELSPAYAHAPTMSITTQPQYGAHIILRKV 518

BLAST of CSPI04G22320 vs. TrEMBL
Match: V7BXP5_PHAVU (Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_005G063200g PE=3 SV=1)

HSP 1 Score: 671.0 bits (1730), Expect = 1.2e-189
Identity = 307/512 (59.96%), Postives = 405/512 (79.10%), Query Frame = 1

Query: 7   TIISIVLVLLLLLGWKLADWIWFRPKKLEKLLRQQGFAGNSYRILHGDLKERAAMREEAM 66
           T ++++++LLL+  WK+ +W+WF PK+LEKLLR+QGF GN Y++L GD KE   MR+EA+
Sbjct: 7   TTLTLIVILLLIWAWKILNWLWFNPKRLEKLLREQGFQGNPYKLLVGDSKEFLKMRKEAL 66

Query: 67  SKPMNFSNHIAPRVIPSIYHTIQRYGKNSFMWLGPIPRVHIMDPEQLKTVFSFFNDFQKP 126
           SKPM+ S+ I PRV   I H+++ +GKNSF+WLGP PRV I+DPEQ+K +F+   DF KP
Sbjct: 67  SKPMDLSDDIVPRVSSYINHSVKSHGKNSFIWLGPTPRVTILDPEQIKDIFNKIYDFPKP 126

Query: 127 TMNPFTKLLVQGIVNLDGEKWVKHRKIINPAFHLEKLKDMVPAFYHSCNEMVSKWESMVS 186
            +NP  KLL  G+ + +GEKW KHR++INPAF+LEKLK M+P F+ SCN++V+KWE M+S
Sbjct: 127 DINPLLKLLATGLADYEGEKWSKHRRLINPAFNLEKLKAMLPLFFESCNDLVNKWERMLS 186

Query: 187 KEGSCELDVMPYLKNMASDVISRTAFGSSYEKGKKIFKLQTELTNFVIQTTLGIYIPGWR 246
            +GSCE+D  P+L+NM SDVI+R+AFGSS+E+G++IF+LQ E     +Q  L I IPGWR
Sbjct: 187 SDGSCEIDAWPFLQNMTSDVIARSAFGSSFEEGRRIFQLQREQAKLALQVILKIQIPGWR 246

Query: 247 FLPTKLNRKMKEISKEITTLILGIMDEREKSMKAGEAIQTDLLSILMESNMNEIKQH--G 306
           FLPTK +R+MKEI ++I   +  ++ +REK++K GEAI  DLL IL+ESN  EI++H  G
Sbjct: 247 FLPTKSHRRMKEIDRDIKASLKDMIYKREKALKKGEAINDDLLGILLESNHKEIQEHAKG 306

Query: 307 NKKDIGMSIEDVIEECKLFYIAGQETTATLLVWTMILLSSYSEWQERARAEVFEIFGNKQ 366
           + K++GMS+EDVIEECKLFY AGQETT+ LLVWTM+LLS Y +WQERAR EV ++FGN++
Sbjct: 307 DNKNVGMSLEDVIEECKLFYFAGQETTSVLLVWTMVLLSRYPDWQERAREEVLQVFGNQK 366

Query: 367 PNYDGLNRLKVVTMIFNEVLRLYPPGSLFVRIVRKETRLGNLTLPGGVMLGLPIVLIQRD 426
           P++DGLNRLK+VTMI NEVLRLYPP     R+ +++ +LGN+ LP GV + LP VL+  +
Sbjct: 367 PSFDGLNRLKIVTMILNEVLRLYPPALGMNRLAKRDLKLGNIILPAGVQIFLPTVLVHHN 426

Query: 427 PELWGEDAHEFNPERFSGGVSKATKNPSAFIPFGWGPRICIGQTFAMIEAKMALSMILQR 486
            E WGEDA +FNPERFS GV KATK   +F PFGWGPRICIGQ F+++EAKMALSMILQ 
Sbjct: 427 CEFWGEDAKQFNPERFSEGVLKATKGRVSFFPFGWGPRICIGQNFSLLEAKMALSMILQH 486

Query: 487 FSFELSPSYTHSPIASLTTQPQHGAHIILHKL 517
           FSFELSP+Y H+P   +T QPQ+GAHIILHK+
Sbjct: 487 FSFELSPAYAHAPAMLITLQPQYGAHIILHKV 518

BLAST of CSPI04G22320 vs. TAIR10
Match: AT3G14690.1 (AT3G14690.1 cytochrome P450, family 72, subfamily A, polypeptide 15)

HSP 1 Score: 596.7 bits (1537), Expect = 1.4e-170
Identity = 281/509 (55.21%), Postives = 377/509 (74.07%), Query Frame = 1

Query: 9   ISIVLVLLLLLGWKLADWIWFRPKKLEKLLRQQGFAGNSYRILHGDLKERAAMREEAMSK 68
           IS+VL ++    W+   W+WF+PK LE  LR+QG AG  Y  L GDLK+   M  EA SK
Sbjct: 10  ISVVLAVVSWWIWRTLQWVWFKPKMLEHYLRRQGLAGTPYTPLVGDLKKNFTMLSEARSK 69

Query: 69  PMNFSNHIAPRVIPSIYHTIQRYGKNSFMWLGPIPRVHIMDPEQLKTVFSFFNDFQKPTM 128
           P+  ++ I+PRV+P      + YG+  F W GPIP + IMDPEQ+K VF+   DFQKP  
Sbjct: 70  PLKLTDDISPRVVPYPLQMFKTYGRTYFTWFGPIPTITIMDPEQIKEVFNKVYDFQKPHT 129

Query: 129 NPFTKLLVQGIVNLDGEKWVKHRKIINPAFHLEKLKDMVPAFYHSCNEMVSKWESMVSKE 188
            P   ++ +G+ N DG+KW KHR+IINPAFH+EK+K+MVPAF+ SC E+V +W+ +VS +
Sbjct: 130 FPLATIIAKGLANYDGDKWAKHRRIINPAFHIEKIKNMVPAFHQSCREVVGEWDQLVSDK 189

Query: 189 GS-CELDVMPYLKNMASDVISRTAFGSSYEKGKKIFKLQTELTNFVIQTTLGIYIPGWRF 248
           GS CE+DV P L +M +DVISRTAFGSSY++G++IF+LQ EL   +IQ     +IPG+ +
Sbjct: 190 GSSCEVDVWPGLVSMTADVISRTAFGSSYKEGQRIFELQAELAQLIIQAFRKAFIPGYSY 249

Query: 249 LPTKLNRKMKEISKEITTLILGIMDEREKSMKAGEAIQTDLLSILMESNMNEIKQHGNKK 308
           LPTK NR+MK  ++EI  ++ GI+++R ++ +AGEA   DLL IL+ESN+ + +  GN  
Sbjct: 250 LPTKSNRRMKAAAREIQVILRGIVNKRLRAREAGEAPSDDLLGILLESNLRQTE--GN-- 309

Query: 309 DIGMSIEDVIEECKLFYIAGQETTATLLVWTMILLSSYSEWQERARAEVFEIFGNKQPNY 368
             GMS ED++EECKLFY AGQETT+ LLVWTM+LLS + +WQ RAR EV ++FG+K+P+ 
Sbjct: 310 --GMSTEDLMEECKLFYFAGQETTSVLLVWTMVLLSQHQDWQARAREEVKQVFGDKEPDA 369

Query: 369 DGLNRLKVVTMIFNEVLRLYPPGSLFVRIVRKETRLGNLTLPGGVMLGLPIVLIQRDPEL 428
           +GLN+LKV+TMI  EVLRLYPP +   R + KE +LG+LTLPGGV + LPI+L+Q D EL
Sbjct: 370 EGLNQLKVMTMILYEVLRLYPPVTQLTRAIHKELKLGDLTLPGGVQISLPILLVQHDIEL 429

Query: 429 WGEDAHEFNPERFSGGVSKATKNPSAFIPFGWGPRICIGQTFAMIEAKMALSMILQRFSF 488
           WG DA EFNP+RF  G+SKATK+  +F PF WGPRICIGQ FA++EAKMA+++IL+RFSF
Sbjct: 430 WGNDAAEFNPDRFKDGLSKATKSQVSFFPFAWGPRICIGQNFALLEAKMAMALILRRFSF 489

Query: 489 ELSPSYTHSPIASLTTQPQHGAHIILHKL 517
           E+SPSY H+P   +T  PQ GA +I+HKL
Sbjct: 490 EISPSYVHAPYTVITIHPQFGAQLIMHKL 512

BLAST of CSPI04G22320 vs. TAIR10
Match: AT3G14610.1 (AT3G14610.1 cytochrome P450, family 72, subfamily A, polypeptide 7)

HSP 1 Score: 588.6 bits (1516), Expect = 3.8e-168
Identity = 276/510 (54.12%), Postives = 371/510 (72.75%), Query Frame = 1

Query: 9   ISIVLVLLLLLGWKLADWIWFRPKKLEKLLRQQGFAGNSYRILHGDLKERAAMREEAMSK 68
           + +++ +++L  W++  W+W +PK LE  L++QG  G  Y  L GD+K    M  EA SK
Sbjct: 9   LPVLVAVVVLWTWRIVKWVWIKPKMLESSLKRQGLTGTPYTPLVGDIKRNVDMMMEARSK 68

Query: 69  PMNFSNHIAPRVIPSIYHTIQRYGKNSFMWLGPIPRVHIMDPEQLKTVFSFFNDFQKPTM 128
           P+N ++ I PR++P     +  +GK  F+W+GP+P + I +PEQ+K VF+  NDF+K + 
Sbjct: 69  PINVTDDITPRLLPLALKMLNSHGKTFFIWIGPLPTIVITNPEQIKEVFNKVNDFEKAST 128

Query: 129 NPFTKLLVQGIVNLDGEKWVKHRKIINPAFHLEKLKDMVPAFYHSCNEMVSKWESMVS-K 188
            P  +LL  G+ +  G+KW  HR+IINPAFHLEK+K+M+PAFYH C+E+V +WE + + K
Sbjct: 129 FPLIRLLAGGLASYKGDKWASHRRIINPAFHLEKIKNMIPAFYHCCSEVVCQWEKLFTDK 188

Query: 189 EGSCELDVMPYLKNMASDVISRTAFGSSYEKGKKIFKLQTELTNFVIQTTLGIYIPGWRF 248
           E   E+DV P+L NM +DVIS TAFGSSY++G++IF+LQ EL   + Q     YIPG RF
Sbjct: 189 ESPLEVDVWPWLVNMTADVISHTAFGSSYKEGQRIFQLQGELAELIAQAFKKSYIPGSRF 248

Query: 249 LPTKLNRKMKEISKEITTLILGIMDEREKSMKAGEAIQTDLLSILMESNMNEIKQHGNKK 308
            PTK NR+MK I +E+  ++ GI+ +REK+ +AGE    DLL IL+ESN  E    GN  
Sbjct: 249 YPTKSNRRMKAIDREVDVILRGIVSKREKAREAGEPANDDLLGILLESNSEE--SQGN-- 308

Query: 309 DIGMSIEDVIEECKLFYIAGQETTATLLVWTMILLSSYSEWQERARAEVFEIFG-NKQPN 368
             GMS+EDV++ECKLFY AGQETT+ LLVWTM+LLS + +WQ RAR EV ++ G N +P+
Sbjct: 309 --GMSVEDVMKECKLFYFAGQETTSVLLVWTMVLLSHHQDWQARAREEVMQVLGENNKPD 368

Query: 369 YDGLNRLKVVTMIFNEVLRLYPPGSLFVRIVRKETRLGNLTLPGGVMLGLPIVLIQRDPE 428
            + LN LKV+TMIFNEVLRLYPP +   R+V KE +LG LTLP G+ + LP +L+QRD E
Sbjct: 369 MESLNNLKVMTMIFNEVLRLYPPVAQLKRVVNKEMKLGELTLPAGIQIYLPTILVQRDTE 428

Query: 429 LWGEDAHEFNPERFSGGVSKATKNPSAFIPFGWGPRICIGQTFAMIEAKMALSMILQRFS 488
           LWG+DA +F PERF  G+SKATKN  +F PFGWGPRICIGQ FAM+EAKMA+++ILQ+FS
Sbjct: 429 LWGDDAADFKPERFRDGLSKATKNQVSFFPFGWGPRICIGQNFAMLEAKMAMALILQKFS 488

Query: 489 FELSPSYTHSPIASLTTQPQHGAHIILHKL 517
           FELSPSY H+P   +TT+PQ GAH+ILHKL
Sbjct: 489 FELSPSYVHAPQTVMTTRPQFGAHLILHKL 512

BLAST of CSPI04G22320 vs. TAIR10
Match: AT3G14660.1 (AT3G14660.1 cytochrome P450, family 72, subfamily A, polypeptide 13)

HSP 1 Score: 587.4 bits (1513), Expect = 8.5e-168
Identity = 280/509 (55.01%), Postives = 376/509 (73.87%), Query Frame = 1

Query: 9   ISIVLVLLLLLGWKLADWIWFRPKKLEKLLRQQGFAGNSYRILHGDLKERAAMREEAMSK 68
           +S+ +V++    W+    +W +PK LE  LR+QG AG  Y  L GDLK   +M  EA SK
Sbjct: 10  VSVAVVVVSWWVWRTLQRVWLKPKMLESYLRRQGLAGTPYTPLVGDLKRNFSMLAEARSK 69

Query: 69  PMNFSNHIAPRVIPSIYHTIQRYGKNSFMWLGPIPRVHIMDPEQLKTVFSFFNDFQKPTM 128
           P+N ++ I PR++P     ++ +G+  F W GPIP + IMDPEQ+K VF+   DFQK   
Sbjct: 70  PINLTDDITPRIVPYPLQMLKTHGRTFFTWFGPIPTITIMDPEQIKEVFNKVYDFQKAHT 129

Query: 129 NPFTKLLVQGIVNLDGEKWVKHRKIINPAFHLEKLKDMVPAFYHSCNEMVSKWESMVS-K 188
            P  +L+  G+V+ DG+KW KHR+IINPAFHLEK+K+MVPAF+ SC+E+V +W+ +V+ K
Sbjct: 130 FPLGRLIAAGLVSYDGDKWTKHRRIINPAFHLEKIKNMVPAFHQSCSEIVGEWDKLVTDK 189

Query: 189 EGSCELDVMPYLKNMASDVISRTAFGSSYEKGKKIFKLQTELTNFVIQTTLGIYIPGWRF 248
           + SCE+D+ P+L +M +DVISRTAFGSSY++G++IF+LQ EL   +IQ      IPG+R+
Sbjct: 190 QSSCEVDIWPWLVSMTADVISRTAFGSSYKEGQRIFELQAELAQLIIQAFRKAIIPGYRY 249

Query: 249 LPTKLNRKMKEISKEITTLILGIMDEREKSMKAGEAIQTDLLSILMESNMNEIKQHGNKK 308
            PTK NR+MK  ++EI  ++ GI+++R ++ +AGEA   DLL IL+ESN+ + K  GN  
Sbjct: 250 FPTKGNRRMKAAAREIKFILRGIVNKRLRAREAGEAPSDDLLGILLESNLGQTK--GN-- 309

Query: 309 DIGMSIEDVIEECKLFYIAGQETTATLLVWTMILLSSYSEWQERARAEVFEIFGNKQPNY 368
             GMS E+++EECKLFY AGQETT  LLVWTM+LLS + +WQ RAR EV ++FG+K+P+ 
Sbjct: 310 --GMSTEELMEECKLFYFAGQETTTVLLVWTMVLLSQHQDWQARAREEVKQVFGDKEPDA 369

Query: 369 DGLNRLKVVTMIFNEVLRLYPPGSLFVRIVRKETRLGNLTLPGGVMLGLPIVLIQRDPEL 428
           +GLN+LKV+TMI  EVLRLYPP     R + KE +LG+LTLPGGV + LPI+LIQRD EL
Sbjct: 370 EGLNQLKVMTMILYEVLRLYPPVVQLTRAIHKEMQLGDLTLPGGVQISLPILLIQRDREL 429

Query: 429 WGEDAHEFNPERFSGGVSKATKNPSAFIPFGWGPRICIGQTFAMIEAKMALSMILQRFSF 488
           WG DA EF P+RF  G+SKATKN  +F PF WGPRICIGQ FA++EAKMA+++IL++FSF
Sbjct: 430 WGNDAGEFKPDRFKDGLSKATKNQVSFFPFAWGPRICIGQNFALLEAKMAMTLILRKFSF 489

Query: 489 ELSPSYTHSPIASLTTQPQHGAHIILHKL 517
           ELSPSY H+P   LTT PQ GA +ILHKL
Sbjct: 490 ELSPSYVHAPYTVLTTHPQFGAPLILHKL 512

BLAST of CSPI04G22320 vs. TAIR10
Match: AT3G14650.1 (AT3G14650.1 cytochrome P450, family 72, subfamily A, polypeptide 11)

HSP 1 Score: 586.6 bits (1511), Expect = 1.5e-167
Identity = 280/509 (55.01%), Postives = 374/509 (73.48%), Query Frame = 1

Query: 9   ISIVLVLLLLLGWKLADWIWFRPKKLEKLLRQQGFAGNSYRILHGDLKERAAMREEAMSK 68
           +S+ +V++    W+   W+WF+PK LE  LR+QG AG  Y  L GDLK+  +MR EA SK
Sbjct: 10  VSVAVVVVSWWVWRTLQWVWFKPKMLESYLRRQGLAGTPYTPLVGDLKKNFSMRAEARSK 69

Query: 69  PMNFSNHIAPRVIPSIYHTIQRYGKNSFMWLGPIPRVHIMDPEQLKTVFSFFNDFQKPTM 128
           P+N ++ I PR++P     ++ +G+  F W G IP + IMDPEQ+  V +   DFQK   
Sbjct: 70  PINLTDDITPRIVPYPLQMLKTHGRTFFTWFGAIPTITIMDPEQITEVLNKVYDFQKAHT 129

Query: 129 NPFTKLLVQGIVNLDGEKWVKHRKIINPAFHLEKLKDMVPAFYHSCNEMVSKWESMVS-K 188
            P  +L+  G+++ DG+KW KHR+IINPAFHLEK+K+MVPAF+ SC+E+V KW+ +VS K
Sbjct: 130 FPLGRLIATGVLSYDGDKWAKHRRIINPAFHLEKIKNMVPAFHQSCSEIVCKWDKLVSDK 189

Query: 189 EGSCELDVMPYLKNMASDVISRTAFGSSYEKGKKIFKLQTELTNFVIQTTLGIYIPGWRF 248
           E SCE+DV P L +M +DVISRTAFGSS  +G++IF+LQ EL   +IQT    +IPG+ +
Sbjct: 190 ESSCEVDVWPGLVSMTADVISRTAFGSSCVEGQRIFELQAELAQLIIQTVRKAFIPGYSY 249

Query: 249 LPTKLNRKMKEISKEITTLILGIMDEREKSMKAGEAIQTDLLSILMESNMNEIKQHGNKK 308
           LPTK NR+MK  ++EI  ++ GI+++R ++ +AGEA   DLL IL+ESN+ + K  GN  
Sbjct: 250 LPTKGNRRMKAKAREIQVILRGIVNKRLRAREAGEAPNDDLLGILLESNLGQTK--GN-- 309

Query: 309 DIGMSIEDVIEECKLFYIAGQETTATLLVWTMILLSSYSEWQERARAEVFEIFGNKQPNY 368
             GMS ED++EECKLFY  GQETT+ LLVWTM+LLS + +WQ RAR EV ++FG+K+P+ 
Sbjct: 310 --GMSTEDLMEECKLFYFVGQETTSVLLVWTMVLLSQHQDWQARAREEVKQVFGDKEPDA 369

Query: 369 DGLNRLKVVTMIFNEVLRLYPPGSLFVRIVRKETRLGNLTLPGGVMLGLPIVLIQRDPEL 428
           +GLN+LKV+TMI  EVLRLYPP     R + KE  LG+LTLPGGV++ LPI+L+QRD EL
Sbjct: 370 EGLNQLKVMTMILYEVLRLYPPIPQLSRAIHKEMELGDLTLPGGVLINLPILLVQRDTEL 429

Query: 429 WGEDAHEFNPERFSGGVSKATKNPSAFIPFGWGPRICIGQTFAMIEAKMALSMILQRFSF 488
           WG DA EF P+RF  G+SKATKN ++F PF WG RICIGQ FA++EAKMA+++ILQRFSF
Sbjct: 430 WGNDAGEFKPDRFKDGLSKATKNQASFFPFAWGSRICIGQNFALLEAKMAMALILQRFSF 489

Query: 489 ELSPSYTHSPIASLTTQPQHGAHIILHKL 517
           ELSPSY H+P    T  PQ GA +I+HKL
Sbjct: 490 ELSPSYVHAPYTVFTIHPQFGAPLIMHKL 512

BLAST of CSPI04G22320 vs. TAIR10
Match: AT3G14680.1 (AT3G14680.1 cytochrome P450, family 72, subfamily A, polypeptide 14)

HSP 1 Score: 584.7 bits (1506), Expect = 5.5e-167
Identity = 279/508 (54.92%), Postives = 375/508 (73.82%), Query Frame = 1

Query: 10  SIVLVLLLLLGWKLADWIWFRPKKLEKLLRQQGFAGNSYRILHGDLKERAAMREEAMSKP 69
           S+ +V++    W+   W+WF PK LE+ LR+QG +G SY  L GD K+  +M  EA SKP
Sbjct: 11  SLAVVVVSWWVWRTLKWVWFTPKMLERSLRRQGLSGTSYTPLIGDFKKMISMFIEATSKP 70

Query: 70  MNFSNHIAPRVIPSIYHTIQRYGKNSFMWLGPIPRVHIMDPEQLKTVFSFFNDFQKPTMN 129
           +  ++ I PRV+P     ++ +G+ +  W GPIP + IMDPEQ+K VF+   DFQK    
Sbjct: 71  IKPTDDITPRVMPHPLQMLKTHGRTNLTWFGPIPTITIMDPEQIKEVFNKVYDFQKAHTF 130

Query: 130 PFTKLLVQGIVNLDGEKWVKHRKIINPAFHLEKLKDMVPAFYHSCNEMVSKWESMVSKEG 189
           P +K+L  G+V+ DG+KW +HR+IINPAFHLEK+K+MV  F+ SC+E+V +W+ +VS +G
Sbjct: 131 PLSKILGTGLVSYDGDKWAQHRRIINPAFHLEKIKNMVHVFHESCSELVGEWDKLVSDKG 190

Query: 190 S-CELDVMPYLKNMASDVISRTAFGSSYEKGKKIFKLQTELTNFVIQTTLGIYIPGWRFL 249
           S CE+DV P L +M +DVISRTAFGSSY +G +IF+LQ EL   V+Q     +IPG+ +L
Sbjct: 191 SSCEVDVWPGLTSMTADVISRTAFGSSYREGHRIFELQAELAQLVMQAFQKFFIPGYIYL 250

Query: 250 PTKLNRKMKEISKEITTLILGIMDEREKSMKAGEAIQTDLLSILMESNMNEIKQHGNKKD 309
           PTK NR+MK  ++EI  ++ GI+++RE++ ++GEA   DLL IL+ESN+ + +  GN   
Sbjct: 251 PTKGNRRMKTAAREIQDILRGIINKRERARESGEAPSEDLLGILLESNLGQTE--GN--- 310

Query: 310 IGMSIEDVIEECKLFYIAGQETTATLLVWTMILLSSYSEWQERARAEVFEIFGNKQPNYD 369
            GMS ED++EECKLFY+AGQETT+ LLVWTM+LLS + +WQ RAR EV ++FG+KQP+ +
Sbjct: 311 -GMSTEDMMEECKLFYLAGQETTSVLLVWTMVLLSQHQDWQARAREEVKQVFGDKQPDTE 370

Query: 370 GLNRLKVVTMIFNEVLRLYPPGSLFVRIVRKETRLGNLTLPGGVMLGLPIVLIQRDPELW 429
           GLN+LKV+TMI  EVLRLYPP     R + KE +LG+LTLPGGV + LP++L+ RD ELW
Sbjct: 371 GLNQLKVMTMILYEVLRLYPPVVQLTRAIHKEMKLGDLTLPGGVQISLPVLLVHRDTELW 430

Query: 430 GEDAHEFNPERFSGGVSKATKNPSAFIPFGWGPRICIGQTFAMIEAKMALSMILQRFSFE 489
           G DA EF PERF  G+SKATKN  +F PF WGPRICIGQ F ++EAKMA+S+ILQRFSFE
Sbjct: 431 GNDAGEFKPERFKDGLSKATKNQVSFFPFAWGPRICIGQNFTLLEAKMAMSLILQRFSFE 490

Query: 490 LSPSYTHSPIASLTTQPQHGAHIILHKL 517
           LSPSY H+P   +T  PQ GAH++LHKL
Sbjct: 491 LSPSYVHAPYTIITLYPQFGAHLMLHKL 512

BLAST of CSPI04G22320 vs. NCBI nr
Match: gi|778695562|ref|XP_011654016.1| (PREDICTED: cytochrome P450 CYP72A219-like [Cucumis sativus])

HSP 1 Score: 1050.4 bits (2715), Expect = 1.0e-303
Identity = 515/516 (99.81%), Postives = 516/516 (100.00%), Query Frame = 1

Query: 1   MWYAPDTIISIVLVLLLLLGWKLADWIWFRPKKLEKLLRQQGFAGNSYRILHGDLKERAA 60
           MWYAPDTIISIVLVLLLLLGWKLADWIWFRPKKLEKLLRQQGFAGNSYRILHGDLKERAA
Sbjct: 1   MWYAPDTIISIVLVLLLLLGWKLADWIWFRPKKLEKLLRQQGFAGNSYRILHGDLKERAA 60

Query: 61  MREEAMSKPMNFSNHIAPRVIPSIYHTIQRYGKNSFMWLGPIPRVHIMDPEQLKTVFSFF 120
           MREEAMSKPMNFSNHIAPRVIPSIYHTIQRYGKNSFMWLGPIPRVHIMDPEQLKTVFSF+
Sbjct: 61  MREEAMSKPMNFSNHIAPRVIPSIYHTIQRYGKNSFMWLGPIPRVHIMDPEQLKTVFSFY 120

Query: 121 NDFQKPTMNPFTKLLVQGIVNLDGEKWVKHRKIINPAFHLEKLKDMVPAFYHSCNEMVSK 180
           NDFQKPTMNPFTKLLVQGIVNLDGEKWVKHRKIINPAFHLEKLKDMVPAFYHSCNEMVSK
Sbjct: 121 NDFQKPTMNPFTKLLVQGIVNLDGEKWVKHRKIINPAFHLEKLKDMVPAFYHSCNEMVSK 180

Query: 181 WESMVSKEGSCELDVMPYLKNMASDVISRTAFGSSYEKGKKIFKLQTELTNFVIQTTLGI 240
           WESMVSKEGSCELDVMPYLKNMASDVISRTAFGSSYEKGKKIFKLQTELTNFVIQTTLGI
Sbjct: 181 WESMVSKEGSCELDVMPYLKNMASDVISRTAFGSSYEKGKKIFKLQTELTNFVIQTTLGI 240

Query: 241 YIPGWRFLPTKLNRKMKEISKEITTLILGIMDEREKSMKAGEAIQTDLLSILMESNMNEI 300
           YIPGWRFLPTKLNRKMKEISKEITTLILGIMDEREKSMKAGEAIQTDLLSILMESNMNEI
Sbjct: 241 YIPGWRFLPTKLNRKMKEISKEITTLILGIMDEREKSMKAGEAIQTDLLSILMESNMNEI 300

Query: 301 KQHGNKKDIGMSIEDVIEECKLFYIAGQETTATLLVWTMILLSSYSEWQERARAEVFEIF 360
           KQHGNKKDIGMSIEDVIEECKLFYIAGQETTATLLVWTMILLSSYSEWQERARAEVFEIF
Sbjct: 301 KQHGNKKDIGMSIEDVIEECKLFYIAGQETTATLLVWTMILLSSYSEWQERARAEVFEIF 360

Query: 361 GNKQPNYDGLNRLKVVTMIFNEVLRLYPPGSLFVRIVRKETRLGNLTLPGGVMLGLPIVL 420
           GNKQPNYDGLNRLKVVTMIFNEVLRLYPPGSLFVRIVRKETRLGNLTLPGGVMLGLPIVL
Sbjct: 361 GNKQPNYDGLNRLKVVTMIFNEVLRLYPPGSLFVRIVRKETRLGNLTLPGGVMLGLPIVL 420

Query: 421 IQRDPELWGEDAHEFNPERFSGGVSKATKNPSAFIPFGWGPRICIGQTFAMIEAKMALSM 480
           IQRDPELWGEDAHEFNPERFSGGVSKATKNPSAFIPFGWGPRICIGQTFAMIEAKMALSM
Sbjct: 421 IQRDPELWGEDAHEFNPERFSGGVSKATKNPSAFIPFGWGPRICIGQTFAMIEAKMALSM 480

Query: 481 ILQRFSFELSPSYTHSPIASLTTQPQHGAHIILHKL 517
           ILQRFSFELSPSYTHSPIASLTTQPQHGAHIILHKL
Sbjct: 481 ILQRFSFELSPSYTHSPIASLTTQPQHGAHIILHKL 516

BLAST of CSPI04G22320 vs. NCBI nr
Match: gi|659103032|ref|XP_008452438.1| (PREDICTED: cytochrome P450 CYP72A219-like [Cucumis melo])

HSP 1 Score: 932.9 bits (2410), Expect = 2.3e-268
Identity = 457/521 (87.72%), Postives = 487/521 (93.47%), Query Frame = 1

Query: 1   MWYAP---DTIIS-IVLVLLLLLGWKLADWIWFRPKKLEKLLRQQGFAGNSYRILHGDLK 60
           M Y P     IIS ++L+LLLLLGWKL DWIWFRPKKLEK+LR+QGFAGNSYRILHGDLK
Sbjct: 1   MSYVPIHIGVIISYVLLLLLLLLGWKLVDWIWFRPKKLEKILRRQGFAGNSYRILHGDLK 60

Query: 61  ERAAMREEAMSKPMNFSNHIAPRVIPSIYHTIQRYGKNSFMWLGPIPRVHIMDPEQLKTV 120
           E AAMREEAMSKPMNFSNHIAPRVIPS+YHTIQ YGK S MWLGP+PRVHIMDPEQLK V
Sbjct: 61  ESAAMREEAMSKPMNFSNHIAPRVIPSVYHTIQLYGKKSMMWLGPMPRVHIMDPEQLKIV 120

Query: 121 FSFFNDFQKPTMNPFTKLLVQGIVNLDGEKWVKHRKIINPAFHLEKLKDMVPAFYHSC-N 180
           FS  NDFQKPTMNP T+LLVQG+ +L+G+KWVKHRKIINPAFH+EKLKDMVPAFYHSC N
Sbjct: 121 FSSINDFQKPTMNPLTRLLVQGLASLEGQKWVKHRKIINPAFHVEKLKDMVPAFYHSCYN 180

Query: 181 EMVSKWESMVSKEGSCELDVMPYLKNMASDVISRTAFGSSYEKGKKIFKLQTELTNFVIQ 240
           EMVSKWE MVS+EGSCELDVMPYL+NMASDVISRTAFGSSYEKGKKIF+LQTEL N VIQ
Sbjct: 181 EMVSKWEGMVSREGSCELDVMPYLQNMASDVISRTAFGSSYEKGKKIFELQTELANLVIQ 240

Query: 241 TTLGIYIPGWRFLPTKLNRKMKEISKEITTLILGIMDEREKSMKAGEAIQTDLLSILMES 300
             LGIYIPGWRFLPTK NRKMKEISKEITTLILGIM+E+EKS+KAGEAIQTDLLSILMES
Sbjct: 241 ANLGIYIPGWRFLPTKSNRKMKEISKEITTLILGIMNEKEKSIKAGEAIQTDLLSILMES 300

Query: 301 NMNEIKQHGNKKDIGMSIEDVIEECKLFYIAGQETTATLLVWTMILLSSYSEWQERARAE 360
           NMNEIKQHGN K+IGMSIEDVIEECKLFYIAGQETTATLLVWTM+LLSSYSEWQERARAE
Sbjct: 301 NMNEIKQHGNNKNIGMSIEDVIEECKLFYIAGQETTATLLVWTMVLLSSYSEWQERARAE 360

Query: 361 VFEIFGNKQPNYDGLNRLKVVTMIFNEVLRLYPPGSLFVRIVRKETRLGNLTLPGGVMLG 420
           VFEIFGNK+P+YDGLNRLKVVTMIFNEVLRLYPP ++F RI+RKETRLGNLTLP GVMLG
Sbjct: 361 VFEIFGNKKPDYDGLNRLKVVTMIFNEVLRLYPPVNMFGRIIRKETRLGNLTLPEGVMLG 420

Query: 421 LPIVLIQRDPELWGEDAHEFNPERFSGGVSKATKNPSAFIPFGWGPRICIGQTFAMIEAK 480
           LP+VLI  DPELWGEDAHEFNPERFS G+SKATKNPSAFIPFGWGPRICIGQ FAMIEAK
Sbjct: 421 LPVVLIHLDPELWGEDAHEFNPERFSEGISKATKNPSAFIPFGWGPRICIGQNFAMIEAK 480

Query: 481 MALSMILQRFSFELSPSYTHSPIASLTTQPQHGAHIILHKL 517
           MALS+ILQRFSFELSPSYTH+PIA LTTQPQHGAHIILHKL
Sbjct: 481 MALSIILQRFSFELSPSYTHAPIAVLTTQPQHGAHIILHKL 521

BLAST of CSPI04G22320 vs. NCBI nr
Match: gi|778695577|ref|XP_004146006.2| (PREDICTED: cytochrome P450 CYP72A219-like [Cucumis sativus])

HSP 1 Score: 905.6 bits (2339), Expect = 4.0e-260
Identity = 431/523 (82.41%), Postives = 478/523 (91.40%), Query Frame = 1

Query: 1   MWYAPDTI-------ISIVLVLLLLLGWKLADWIWFRPKKLEKLLRQQGFAGNSYRILHG 60
           MWY P  I        S+VL+LLL LGWKLADWIWF P++LE+LLR+QGF GNSY+ILHG
Sbjct: 1   MWYVPHMISTPIVIITSLVLLLLLFLGWKLADWIWFGPRRLEQLLRRQGFTGNSYKILHG 60

Query: 61  DLKERAAMREEAMSKPMNFSNHIAPRVIPSIYHTIQRYGKNSFMWLGPIPRVHIMDPEQL 120
           D+KE AAMR+EA+SKPM FSNHIAPRVIPS+YHTIQRYGKNS MWLGP+PRVHIMDPEQL
Sbjct: 61  DMKESAAMRKEALSKPMKFSNHIAPRVIPSVYHTIQRYGKNSMMWLGPLPRVHIMDPEQL 120

Query: 121 KTVFSFFNDFQKPTMNPFTKLLVQGIVNLDGEKWVKHRKIINPAFHLEKLKDMVPAFYHS 180
           KTVFSFFNDFQ+PT +PF +LL+ G+V+L+GEKWVKHRKIINPAFHLEKLK MVPAFYHS
Sbjct: 121 KTVFSFFNDFQRPTTDPFIRLLIHGLVSLEGEKWVKHRKIINPAFHLEKLKAMVPAFYHS 180

Query: 181 CNEMVSKWESMVSKEGSCELDVMPYLKNMASDVISRTAFGSSYEKGKKIFKLQTELTNFV 240
           CNEMVSKWESMVS+EGSCELDVMPYL+NMA+DVISRTAFGSSYE+GKKIFKLQTELTN V
Sbjct: 181 CNEMVSKWESMVSREGSCELDVMPYLQNMAADVISRTAFGSSYERGKKIFKLQTELTNLV 240

Query: 241 IQTTLGIYIPGWRFLPTKLNRKMKEISKEITTLILGIMDEREKSMKAGEAIQTDLLSILM 300
           IQ +L  YIPGWRFLPTK NRK+KE ++EITTL+LGI++E+EKSMKAGE IQTDLLSILM
Sbjct: 241 IQASLETYIPGWRFLPTKSNRKIKETNREITTLVLGIINEKEKSMKAGEEIQTDLLSILM 300

Query: 301 ESNMNEIKQHGNKKDIGMSIEDVIEECKLFYIAGQETTATLLVWTMILLSSYSEWQERAR 360
           ESNMNEIK   N KD+GMSI+ VIEEC+LFYIAGQETTATLL+WTM+LLSSYS+WQERAR
Sbjct: 301 ESNMNEIKHGRNSKDVGMSIQAVIEECRLFYIAGQETTATLLIWTMVLLSSYSDWQERAR 360

Query: 361 AEVFEIFGNKQPNYDGLNRLKVVTMIFNEVLRLYPPGSLFVRIVRKETRLGNLTLPGGVM 420
           AEVFEIFGNK+P+YDGLNRLKVVTMIFNEVLRLYPP S+  R  RKET+LGN+TLPGG+M
Sbjct: 361 AEVFEIFGNKKPDYDGLNRLKVVTMIFNEVLRLYPPVSMLGRTTRKETKLGNMTLPGGIM 420

Query: 421 LGLPIVLIQRDPELWGEDAHEFNPERFSGGVSKATKNPSAFIPFGWGPRICIGQTFAMIE 480
           L LPI+LIQRDPELWGEDAHEFNPERFS GV KATKNP AF+PFGWGPRICIGQ FAMIE
Sbjct: 421 LSLPIILIQRDPELWGEDAHEFNPERFSEGVFKATKNPGAFMPFGWGPRICIGQNFAMIE 480

Query: 481 AKMALSMILQRFSFELSPSYTHSPIASLTTQPQHGAHIILHKL 517
           AKMALSMILQ FSFELSPSYTH+P A+LTTQPQHGAHIILHKL
Sbjct: 481 AKMALSMILQHFSFELSPSYTHTPFAALTTQPQHGAHIILHKL 523

BLAST of CSPI04G22320 vs. NCBI nr
Match: gi|778695569|ref|XP_011654017.1| (PREDICTED: cytochrome P450 CYP72A219 isoform X2 [Cucumis sativus])

HSP 1 Score: 889.4 bits (2297), Expect = 3.0e-255
Identity = 429/514 (83.46%), Postives = 472/514 (91.83%), Query Frame = 1

Query: 8   IISIVLVL---LLLLGWKLADWIWFRPKKLEKLLRQQGFAGNSYRILHGDLKERAAMREE 67
           I+ IV++L   LLLLGWKL DWIWFRPKKLEKLLRQQGF GNSYRILHGDLKERAAMR++
Sbjct: 13  IVVIVVLLSSSLLLLGWKLVDWIWFRPKKLEKLLRQQGFTGNSYRILHGDLKERAAMRDQ 72

Query: 68  AMSKPMNFSNHIAPRVIPSIYHTIQRYGKNSFMWLGPIPRVHIMDPEQLKTVFSFFNDFQ 127
           A+SKPMNFSN+IAPRVIPS++HTIQ YGKNSFMW+GP+PRVHIMDPEQLKTVFS  ND+Q
Sbjct: 73  AISKPMNFSNYIAPRVIPSVHHTIQHYGKNSFMWIGPMPRVHIMDPEQLKTVFSLINDYQ 132

Query: 128 KPT--MNPFTKLLVQGIVNLDGEKWVKHRKIINPAFHLEKLKDMVPAFYHSCNEMVSKWE 187
           KPT  +NP  KLL  G++N +G+KWVKHRKIINPAFHLEKLKDMVPAFYHSC+EM+SKWE
Sbjct: 133 KPTASLNPLAKLLADGLLNHEGQKWVKHRKIINPAFHLEKLKDMVPAFYHSCDEMISKWE 192

Query: 188 SMVSKEGSCELDVMPYLKNMASDVISRTAFGSSYEKGKKIFKLQTELTNFVIQTTLGIYI 247
           SMVS EG CELDVMPYL+NM +DVISRTAFGSSYEKGKKIFKLQTEL   V+Q+TLGIYI
Sbjct: 193 SMVSVEGFCELDVMPYLQNMTADVISRTAFGSSYEKGKKIFKLQTELGEMVMQSTLGIYI 252

Query: 248 PGWRFLPTKLNRKMKEISKEITTLILGIMDEREKSMKAGEAIQTDLLSILMESNMNEIKQ 307
           PGWRFLPTK N KMK ISKEI+TLI+ I++ERE SMK+GEAIQTDLLSILMESN+NEIKQ
Sbjct: 253 PGWRFLPTKSNNKMKVISKEISTLIMDIINERETSMKSGEAIQTDLLSILMESNLNEIKQ 312

Query: 308 HGNKKDIGMSIEDVIEECKLFYIAGQETTATLLVWTMILLSSYSEWQERARAEVFEIFGN 367
           HGN KDIGMSIEDVI+ECKLFYIAGQETTATLL WTM+LLSSYSEWQERARAEVFEIFGN
Sbjct: 313 HGNNKDIGMSIEDVIDECKLFYIAGQETTATLLTWTMVLLSSYSEWQERARAEVFEIFGN 372

Query: 368 KQPNYDGLNRLKVVTMIFNEVLRLYPPGSLFVRIVRKETRLGNLTLPGGVMLGLPIVLIQ 427
           K+P+YDGL+RLKVVTMI NEVLRLYPP S+F R+V+KET+LG LTLP GVMLGLP+VL+Q
Sbjct: 373 KKPDYDGLSRLKVVTMILNEVLRLYPPVSMFGRLVKKETKLGKLTLPAGVMLGLPVVLMQ 432

Query: 428 RDPELWGEDAHEFNPERFSGGVSKATKNPSAFIPFGWGPRICIGQTFAMIEAKMALSMIL 487
            DPELWGEDAHEF PERFS GVSKA KNP AF+PFGWGPRICIG  FAMIEAKMALSMIL
Sbjct: 433 CDPELWGEDAHEFKPERFSEGVSKAAKNPGAFVPFGWGPRICIGLNFAMIEAKMALSMIL 492

Query: 488 QRFSFELSPSYTHSPIASLTTQPQHGAHIILHKL 517
           QRFSF+LSPSYTH+PIA LTTQPQHGAHIILHKL
Sbjct: 493 QRFSFQLSPSYTHTPIAGLTTQPQHGAHIILHKL 526

BLAST of CSPI04G22320 vs. NCBI nr
Match: gi|778695565|ref|XP_004146005.2| (PREDICTED: cytochrome P450 CYP72A219 isoform X1 [Cucumis sativus])

HSP 1 Score: 883.2 bits (2281), Expect = 2.1e-253
Identity = 426/514 (82.88%), Postives = 470/514 (91.44%), Query Frame = 1

Query: 8   IISIVLVL---LLLLGWKLADWIWFRPKKLEKLLRQQGFAGNSYRILHGDLKERAAMREE 67
           I+ IV++L   LLLLGWKL DWIWFRPKKLEKLLRQQGF GNSYRILHGDLKERAAMR++
Sbjct: 13  IVVIVVLLSSSLLLLGWKLVDWIWFRPKKLEKLLRQQGFTGNSYRILHGDLKERAAMRDQ 72

Query: 68  AMSKPMNFSNHIAPRVIPSIYHTIQRYGKNSFMWLGPIPRVHIMDPEQLKTVFSFFNDFQ 127
           A+SKPMNFSN+IAPRVIPS++HTIQ YGKNSFMW+GP+PRVHIMDPEQLKTVFS  ND+Q
Sbjct: 73  AISKPMNFSNYIAPRVIPSVHHTIQHYGKNSFMWIGPMPRVHIMDPEQLKTVFSLINDYQ 132

Query: 128 KPT--MNPFTKLLVQGIVNLDGEKWVKHRKIINPAFHLEKLKDMVPAFYHSCNEMVSKWE 187
           KPT  +NP  KLL  G++N +G+KWVKHRKIINPAFHLEKLKDMVPAFYHSC+EM+SKWE
Sbjct: 133 KPTASLNPLAKLLADGLLNHEGQKWVKHRKIINPAFHLEKLKDMVPAFYHSCDEMISKWE 192

Query: 188 SMVSKEGSCELDVMPYLKNMASDVISRTAFGSSYEKGKKIFKLQTELTNFVIQTTLGIYI 247
           SMVS EG CELDVMPYL+NM +DVISRTAFGSSYEKGKKIFKLQTEL   V+Q+TLGIYI
Sbjct: 193 SMVSVEGFCELDVMPYLQNMTADVISRTAFGSSYEKGKKIFKLQTELGEMVMQSTLGIYI 252

Query: 248 PGWRFLPTKLNRKMKEISKEITTLILGIMDEREKSMKAGEAIQTDLLSILMESNMNEIKQ 307
           PGWRFLPTK N KMK ISKEI+TLI+ I++ERE SMK+GEAIQTDLLSILMESN+NEIKQ
Sbjct: 253 PGWRFLPTKSNNKMKVISKEISTLIMDIINERETSMKSGEAIQTDLLSILMESNLNEIKQ 312

Query: 308 HGNKKDIGMSIEDVIEECKLFYIAGQETTATLLVWTMILLSSYSEWQERARAEVFEIFGN 367
           HGN KDIGMSIEDVI+ECKLFYIAGQETTATLL WTM+LLSSYSEWQERARAEVFEIFGN
Sbjct: 313 HGNNKDIGMSIEDVIDECKLFYIAGQETTATLLTWTMVLLSSYSEWQERARAEVFEIFGN 372

Query: 368 KQPNYDGLNRLKVVTMIFNEVLRLYPPGSLFVRIVRKETRLGNLTLPGGVMLGLPIVLIQ 427
           K+P+YDGL+RLKVVTMI NEVLRLYPP S+F R+V+KET+LG LTLP GVMLGLP+VL+Q
Sbjct: 373 KKPDYDGLSRLKVVTMILNEVLRLYPPVSMFGRLVKKETKLGKLTLPAGVMLGLPVVLMQ 432

Query: 428 RDPELWGEDAHEFNPERFSGGVSKATKNPSAFIPFGWGPRICIGQTFAMIEAKMALSMIL 487
            DPELWGEDAHEF PERFS GVSKA KNP AF+PFGWGPRICIG  FAMIEAKM LSMIL
Sbjct: 433 CDPELWGEDAHEFKPERFSEGVSKAAKNPGAFVPFGWGPRICIGLNFAMIEAKMTLSMIL 492

Query: 488 QRFSFELSPSYTHSPIASLTTQPQHGAHIILHKL 517
           QRFS ELS SYTH+PIA++TTQPQHGAHIILHKL
Sbjct: 493 QRFSLELSSSYTHAPIAAITTQPQHGAHIILHKL 526