CSPI04G20830 (gene) Wild cucumber (PI 183967)

BLAST of CSPI04G20830 vs. Swiss-Prot
Match: GACP3_ARATH (Gamma-tubulin complex component 3 OS=Arabidopsis thaliana GN=GCP3 PE=1 SV=1)

HSP 1 Score: 1273.8 bits (3295), Expect = 0.0e+00
Identity = 646/844 (76.54%), Postives = 730/844 (86.49%), Query Frame = 1

Query: 1   MEEDDSTKVLDLIKDLVLRLLSHNP-TSNSSPTSSDFQKSLRYAIRILTSRMTPSIAPDA 60
           ME+DD  K  DL+++LVLRL+S NP T N  P S  F K+LRYA RIL+SR+TPS+ PDA
Sbjct: 1   MEDDDQQKAADLVQELVLRLVSQNPQTPNLDPNSPAFLKTLRYAFRILSSRLTPSVLPDA 60

Query: 61  AAIAESIKRRLATEGKSSQALTFADLYTKFASKTGPGSVNNKWAVLYLLKIVAEDRKCRQ 120
            AIAES+KRRLAT+GKSS AL FADLYTKFASKTGPGSVNNKWA++YLLKIV++DRK   
Sbjct: 61  TAIAESLKRRLATQGKSSDALAFADLYTKFASKTGPGSVNNKWALVYLLKIVSDDRKSAI 120

Query: 121 TQFESSMLLPNLVASDPVLGKKS-GAAPEKEWQKGVLLVAKDPENLRDVAFKEFSNLLKE 180
              +SS+LLPNL   D   G  S G A +K+W  GVLLV+KDPENLRD+AF+E++ L+KE
Sbjct: 121 NGLDSSVLLPNLGIGDTGNGVLSRGEAKKKDWSNGVLLVSKDPENLRDIAFREYAILVKE 180

Query: 181 ENEVTEEVLVRDVLYACQGIDGKYVKFDNNSDGYVLSNLVKASRATRTMVRKLCEMGWLF 240
           ENEVTEEVLVRDVLYA QGIDGKYVKF++  DGY +   VK  RATR MVR L E+GWLF
Sbjct: 181 ENEVTEEVLVRDVLYASQGIDGKYVKFNSEIDGYAVQESVKVPRATRIMVRMLSELGWLF 240

Query: 241 RKVKGYISESMERFPAEDIGTVGHAFCAALQDELSEYYKLLAILEAQSMNPIPLVSEAAS 300
           RKVK +I+ESM+RFPAED+GTVG AFCAALQDELS+YYKLLA+LEAQ+MNPIPLVSE+AS
Sbjct: 241 RKVKTFITESMDRFPAEDVGTVGQAFCAALQDELSDYYKLLAVLEAQAMNPIPLVSESAS 300

Query: 301 SGNYLSLRRLAVWFAEPMAKVRLMAVLVDKCRVLKGGTMAGAIHLHAQHGDPLVLEFMRR 360
           S NYLSLRRL+VWFAEPM K+RLMAVLVDKC+VL+GG MAGAIHLHAQHGDPLV +FM  
Sbjct: 301 SNNYLSLRRLSVWFAEPMVKMRLMAVLVDKCKVLRGGAMAGAIHLHAQHGDPLVHDFMMS 360

Query: 361 LLRRVCSPLFEMVRSWVLEGELEDIFSEFFVVGQQVKAESLWREGYRLHAGMLPSFISQS 420
           LLR VCSPLFEMVRSWVLEGELED F EFFVVGQ VK + LWREGY+LH  MLPSFIS S
Sbjct: 361 LLRCVCSPLFEMVRSWVLEGELEDTFGEFFVVGQPVKVDLLWREGYKLHPAMLPSFISPS 420

Query: 421 LAQRILRTGKSINFLRVCCEDMGWADAATEAAVAAGTTTKRGGLGYGETDALESLVDEAA 480
           LAQRILRTGKSINFLRVCC+D GWADAA+EAA A+GTTT+RGGLGYGETDALE LV EAA
Sbjct: 421 LAQRILRTGKSINFLRVCCDDHGWADAASEAAAASGTTTRRGGLGYGETDALEHLVTEAA 480

Query: 481 KRIDKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGPELSEPANAISSFKLS 540
           KRIDKHLLDV++KRYKFK+HCLAIKRYLLLGQGDFVQYLMDIVGP+LSEPAN ISSF+L+
Sbjct: 481 KRIDKHLLDVLYKRYKFKEHCLAIKRYLLLGQGDFVQYLMDIVGPKLSEPANNISSFELA 540

Query: 541 GLLETAIRSSNAQYDDPDILDRLKVKMMPHGTGDRGWDVFSLEYEARVPLDTVFTESVMS 600
           G LE AIR+SNAQYDD D+LDRL+VKMMPHG+GDRGWDVFSLEYEARVPLDTVFTESV+S
Sbjct: 541 GFLEAAIRASNAQYDDRDMLDRLRVKMMPHGSGDRGWDVFSLEYEARVPLDTVFTESVLS 600

Query: 601 KYLRIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLTKLHHGVKLQLLSTLRRCQVLWVE 660
           KYLR+FNFLWKL+RVEHALIG WKTMKPNCITS S  KL   VKLQLLS LRRCQVLW E
Sbjct: 601 KYLRVFNFLWKLKRVEHALIGIWKTMKPNCITSNSFVKLQSSVKLQLLSALRRCQVLWNE 660

Query: 661 MNHFVTNLQYYIMFEVLEVSWSDFSNEMEAAMDLDDLLAAHEKYLHSIFEKSLLGEQSQT 720
           MNHFVTN QYYIMFEVLEVSWS+FS EMEAA DLDDLLAAHEKYL++I  KSLLGEQSQT
Sbjct: 661 MNHFVTNFQYYIMFEVLEVSWSNFSKEMEAAKDLDDLLAAHEKYLNAIVGKSLLGEQSQT 720

Query: 721 LCKSLFVLFDIILRFRSHADRLYEGIHELQCRTIESSLPSRDKSKKSRTTERSLETASWI 780
           + +SLFVLF++ILRFRSHADRLYEGIHELQ R+ ES    R+K+K       S E  SWI
Sbjct: 721 IRESLFVLFELILRFRSHADRLYEGIHELQIRSKES---GREKNK-------SQEPGSWI 780

Query: 781 ADGKKALTQRAGEFLRNVEQDLAALAKEYSSLLEEFISQLPLQQHVDLKFLLFRLDFTEF 840
           ++G+K LTQRAGEFL+++ QD+ ++AKEY+S L+ F+S LPLQQ VDLKFL FRLDFTEF
Sbjct: 781 SEGRKGLTQRAGEFLQSMSQDMDSIAKEYTSSLDGFLSLLPLQQSVDLKFLFFRLDFTEF 834

Query: 841 YSQL 843
           YS+L
Sbjct: 841 YSRL 834

BLAST of CSPI04G20830 vs. Swiss-Prot
Match: GCP3_XENLA (Gamma-tubulin complex component 3 homolog OS=Xenopus laevis GN=tubgcp3 PE=1 SV=1)

HSP 1 Score: 461.5 bits (1186), Expect = 2.0e-128
Identity = 259/670 (38.66%), Postives = 382/670 (57.01%), Query Frame = 1

Query: 181 EVTEEVLVRDVLYACQGIDGKYVKFDNNSDGYVLSNLVKASRATRTMVRKLCEMGWLFRK 240
           E+TE  LVRD+LY  QGIDGK+VK  N+ + Y +   V  S++ + +  KL E+GWL  K
Sbjct: 243 EITETSLVRDLLYVFQGIDGKFVKMCNSENCYKVDGKVAVSKSLKDITSKLSELGWLHNK 302

Query: 241 VKGYISE-SMERFPAEDIGTVGHAFCAALQDELSEYYKLLAILEAQ-SMNPIPLVSEAAS 300
           +K Y  + S++R      G VG +FCAAL  EL EYY+LL++L +Q  +     V+    
Sbjct: 303 IKKYTDQRSLDRA----FGLVGQSFCAALHQELKEYYRLLSVLHSQLQVEDDQGVNLGVE 362

Query: 301 SGNYLSLRRLAVWFAEPMAKVRLMAVLVDKCRVLKGGTMAGAIHLHAQHGDPLVLEFMRR 360
           S   L+LRRL VW  +P  +++ +A LVD C+  KGG +A A+H + + GDP +   ++ 
Sbjct: 363 SS--LTLRRLLVWTFDPKIRLKTLAALVDHCQGRKGGELASAVHAYTKTGDPYMRSLVQH 422

Query: 361 LLRRVCSPLFEMVRSWVLEGELEDIFSEFFVVGQQV-KAESLWREGYRLHAGMLPSFISQ 420
           +L  V  P+   +  W+ +GELED + EFFV    V K + LW + Y L   M+PSF++ 
Sbjct: 423 ILGLVAYPILNFLYRWIYDGELEDTYHEFFVASDPVVKTDRLWHDKYSLRKSMIPSFMTM 482

Query: 421 SLAQRILRTGKSINFLRVCCEDMGWADAATEAAVAAGTTTKRGGLGYGETDALESLVDEA 480
             ++++L  GKSINFL   C D   A  A     +A +      L     +A ++ +D A
Sbjct: 483 DQSRKVLLIGKSINFLHQVCHDQTPASKAMAVGKSAESPKDAAELFTDLENAFQTKIDAA 542

Query: 481 AKRIDKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGPELSEPANAISSFKL 540
                K+LLDV++K Y   +H  A++RYLLLGQGDF+++LMD++ PEL  PA  +    L
Sbjct: 543 YFDTSKYLLDVLNKNYNLLEHMQAMRRYLLLGQGDFIRHLMDLLKPELVRPATTLYQHNL 602

Query: 541 SGLLETAIRSSNAQYDDPDILDRLKVKMMPHGTGDRGWDVFSLEYEARVPLDTVFTESVM 600
           +G+LETA+R++NAQ+D+P+IL RL V+++    GD GWDVFSL+Y    P+ TVFT   M
Sbjct: 603 TGILETAVRATNAQFDNPEILKRLDVRLLEVSPGDTGWDVFSLDYHVDGPIATVFTRECM 662

Query: 601 SKYLRIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLTKLHHGVKLQLLSTLRRCQVLWV 660
           S YLR+FNFLW+ +R+E+ L   WK    N        KL  G+  +L   L +C +L  
Sbjct: 663 SHYLRVFNFLWRAKRMEYILTDIWKGHMCN-------AKLLKGMP-ELSGVLHQCHILAS 722

Query: 661 EMNHFVTNLQYYIMFEVLEVSWSDFSNEMEAAMDLDDLLAAHEKYLHSIFEKSLLGEQSQ 720
           EM HF+  +QYYI FEVLE SW +  N++  A DLD ++AAH+ +L +I  + LL  +S+
Sbjct: 723 EMVHFIHQMQYYITFEVLECSWDELWNKVLKAQDLDHIIAAHDVFLDTIISRCLLDSESR 782

Query: 721 TLCKSLFVLFDIILRFRSHADRLYE-GIHELQCRTIESSLPSRDKSKKSRTTERSLETAS 780
            L   L  +FD I+ F++  D LY   + ELQ R         ++ KK R +E      +
Sbjct: 783 ALLNQLRAVFDQIIEFQNAQDALYRAALEELQQRL------QFEERKKERESEGEWGVTA 842

Query: 781 WIADGKKALTQRAGEFLRNVEQDLAALAKEYSSLLEEFISQLPLQQHVDLKFLLFRLDFT 840
              D +    Q   E +  +   L  L   Y  ++++F+  L       L+FL FRLDF 
Sbjct: 843 AEEDVENKRIQEFQESIPKMRSQLRILTHFYQGIVQQFLVLLTTSTDESLRFLSFRLDFN 892

Query: 841 EFYSQLRPHV 847
           E Y    P +
Sbjct: 903 EHYKAREPRL 892

BLAST of CSPI04G20830 vs. Swiss-Prot
Match: GCP3_HUMAN (Gamma-tubulin complex component 3 OS=Homo sapiens GN=TUBGCP3 PE=1 SV=2)

HSP 1 Score: 447.2 bits (1149), Expect = 4.0e-124
Identity = 255/675 (37.78%), Postives = 376/675 (55.70%), Query Frame = 1

Query: 181 EVTEEVLVRDVLYACQGIDGKYVKFDNNSDGYVLSNLVKASRATRTMVRKLCEMGWLFRK 240
           E+TE  LVRD+LY  QGIDGK +K +N  + Y +      SR+ R    +L E+GWL  K
Sbjct: 244 EITEAALVRDILYVFQGIDGKNIKMNNTENCYKVEGKANLSRSLRDTAVRLSELGWLHNK 303

Query: 241 VKGYISE-SMERFPAEDIGTVGHAFCAALQDELSEYYKLLAILEAQ-SMNPIPLVSEAAS 300
           ++ Y  + S++R      G VG +FCAAL  EL EYY+LL++L +Q  +     V+    
Sbjct: 304 IRRYTDQRSLDR----SFGLVGQSFCAALHQELREYYRLLSVLHSQLQLEDDQGVNLGLE 363

Query: 301 SGNYLSLRRLAVWFAEPMAKVRLMAVLVDKCRVLKGGTMAGAIHLHAQHGDPLVLEFMRR 360
           S   L+LRRL VW  +P  +++ +A LVD C+  KGG +A A+H + + GDP +   ++ 
Sbjct: 364 SS--LTLRRLLVWTYDPKIRLKTLAALVDHCQGRKGGELASAVHAYTKTGDPYMRSLVQH 423

Query: 361 LLRRVCSPLFEMVRSWVLEGELEDIFSEFFVVGQQ-VKAESLWREGYRLHAGMLPSFISQ 420
           +L  V  P+   +  W+ +GELED + EFFV     VK + LW + Y L   M+PSF++ 
Sbjct: 424 ILSLVSHPVLSFLYRWIYDGELEDTYHEFFVASDPTVKTDRLWHDKYTLRKSMIPSFMTM 483

Query: 421 SLAQRILRTGKSINFLRVCCEDMGWADAATEAAVAAGTTTKRGGLGYGETDALESLVDEA 480
             ++++L  GKSINFL   C D            +A +      L     +A +  +D A
Sbjct: 484 DQSRKVLLIGKSINFLHQVCHDQTPTTKMIAVTKSAESPQDAADLFTDLENAFQGKIDAA 543

Query: 481 AKRIDKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGPELSEPANAISSFKL 540
                K+LLDV++K+Y   DH  A++RYLLLGQGDF+++LMD++ PEL  PA  +    L
Sbjct: 544 YFETSKYLLDVLNKKYSLLDHMQAMRRYLLLGQGDFIRHLMDLLKPELVRPATTLYQHNL 603

Query: 541 SGLLETAIRSSNAQYDDPDILDRLKVKMMPHGTGDRGWDVFSLEYEARVPLDTVFTESVM 600
           +G+LETA+R++NAQ+D P+IL RL V+++    GD GWDVFSL+Y    P+ TVFT   M
Sbjct: 604 TGILETAVRATNAQFDSPEILRRLDVRLLEVSPGDTGWDVFSLDYHVDGPIATVFTRECM 663

Query: 601 SKYLRIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLTKLHHGVKLQLLSTLRRCQVLWV 660
           S YLR+FNFLW+ +R+E+ L    K    N        KL   +  +    L +C +L  
Sbjct: 664 SHYLRVFNFLWRAKRMEYILTDIRKGHMCN-------AKLLRNMP-EFSGVLHQCHILAS 723

Query: 661 EMNHFVTNLQYYIMFEVLEVSWSDFSNEMEAAMDLDDLLAAHEKYLHSIFEKSLLGEQSQ 720
           EM HF+  +QYYI FEVLE SW +  N+++ A DLD ++AAHE +L +I  + LL   S+
Sbjct: 724 EMVHFIHQMQYYITFEVLECSWDELWNKVQQAQDLDHIIAAHEVFLDTIISRCLLDSDSR 783

Query: 721 TLCKSLFVLFDIILRFRSHADRLYE-GIHELQCRTIESSLPSRDKSKKSRTTERSLETAS 780
            L   L  +FD I+  ++  D +Y   + ELQ R         ++ KK R  E       
Sbjct: 784 ALLNQLRAVFDQIIELQNAQDAIYRAALEELQRRL------QFEEKKKQREIE-----GQ 843

Query: 781 W--IADGKKALTQRAGEFLRNVEQ---DLAALAKEYSSLLEEFISQLPLQQHVDLKFLLF 840
           W   A  ++   +R GEF  ++ +    L  L   Y  ++++F+  L       L+FL F
Sbjct: 844 WGVTAAEEEEENKRIGEFKESIPKMCSQLRILTHFYQGIVQQFLVLLTTSSDESLRFLSF 893

Query: 841 RLDFTEFYSQLRPHV 847
           RLDF E Y    P +
Sbjct: 904 RLDFNEHYKAREPRL 893

BLAST of CSPI04G20830 vs. Swiss-Prot
Match: GCP3_MOUSE (Gamma-tubulin complex component 3 OS=Mus musculus GN=Tubgcp3 PE=1 SV=2)

HSP 1 Score: 436.8 bits (1122), Expect = 5.4e-121
Identity = 247/670 (36.87%), Postives = 370/670 (55.22%), Query Frame = 1

Query: 181 EVTEEVLVRDVLYACQGIDGKYVKFDNNSDGYVLSNLVKASRATRTMVRKLCEMGWLFRK 240
           EVTE  LVRD+LY  QGIDGK +K  +  + Y +      +++ R    +L E+GWL  K
Sbjct: 242 EVTEAALVRDILYVFQGIDGKNIKMSSTENCYKVEAKANLNKSLRDTAVRLAELGWLHNK 301

Query: 241 VKGYISE-SMERFPAEDIGTVGHAFCAALQDELSEYYKLLAILEAQ-SMNPIPLVSEAAS 300
           ++ Y  + S++R      G VG +FCAAL  EL EYY+LL++L +Q  +     V+    
Sbjct: 302 IRKYADQRSLDR----SFGLVGQSFCAALHQELKEYYRLLSVLHSQLQLEDDQGVNLGLE 361

Query: 301 SGNYLSLRRLAVWFAEPMAKVRLMAVLVDKCRVLKGGTMAGAIHLHAQHGDPLVLEFMRR 360
           S   L+LRRL VW  +P  +++ +A LVD C+  KGG +A A+H + + GDP +   ++ 
Sbjct: 362 SS--LTLRRLLVWTYDPKIRLKTLAALVDHCQGRKGGELASAVHAYTKTGDPYMKSLVQH 421

Query: 361 LLRRVCSPLFEMVRSWVLEGELEDIFSEFFVVGQQ-VKAESLWREGYRLHAGMLPSFISQ 420
           +L  V  P+   +  W+ +GELED + EFFV     VK + LW + Y L   M+PSFI+ 
Sbjct: 422 ILSLVSHPVLSFLYRWIYDGELEDTYHEFFVASDPTVKTDRLWHDKYTLRKSMIPSFITM 481

Query: 421 SLAQRILRTGKSINFLRVCCEDMGWADAATEAAVAAGTTTKRGGLGYGETDALESLVDEA 480
             ++++L  GKSINFL   C D            +A +      L     +A +  +D A
Sbjct: 482 DQSRKVLLIGKSINFLHQVCHDQTPTTKMIAVTKSAESPRDAADLFTDLENAFQGKIDAA 541

Query: 481 AKRIDKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGPELSEPANAISSFKL 540
                K+LLDV++K+Y   +H  A++RYLLLGQGDF+++LMD++ PEL  PA  +    L
Sbjct: 542 YFETSKYLLDVLNKKYSLLEHMQAMRRYLLLGQGDFIRHLMDLLKPELVRPATTLYQHNL 601

Query: 541 SGLLETAIRSSNAQYDDPDILDRLKVKMMPHGTGDRGWDVFSLEYEARVPLDTVFTESVM 600
           +G+LETA+R++NAQ+D P+IL RL V+++    GD GWDVFSL+Y    P+ TVFT   M
Sbjct: 602 TGILETAVRATNAQFDSPEILKRLDVRLLEVSPGDTGWDVFSLDYHVDGPIATVFTRECM 661

Query: 601 SKYLRIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLTKLHHGVKLQLLSTLRRCQVLWV 660
           S YLR+FNFLW+ +R+E+ L    K    N        KL   +  +    L +C +L  
Sbjct: 662 SHYLRVFNFLWRAKRMEYILTDIRKGHMCN-------AKLLRNMP-EFSGVLHQCHILAS 721

Query: 661 EMNHFVTNLQYYIMFEVLEVSWSDFSNEMEAAMDLDDLLAAHEKYLHSIFEKSLLGEQSQ 720
           EM HF+  +QYYI FEVLE SW +  N ++ A DLD ++AAHE +L++I  + LL   S+
Sbjct: 722 EMVHFIHQMQYYITFEVLECSWDELWNRVQQAQDLDHIIAAHEAFLNTITSRCLLDSNSR 781

Query: 721 TLCKSLFVLFDIILRFRSHADRLYE-GIHELQCRTIESSLPSRDKSKKSRTTERSLETAS 780
            L   L  +FD I+  ++  D +Y   + ELQ R         ++ KK R  E      +
Sbjct: 782 VLLNQLRAVFDQIIELQNAQDVMYRAALEELQRRL------QFEEKKKQREIEGQWGVTA 841

Query: 781 WIADGKKALTQRAGEFLRNVEQDLAALAKEYSSLLEEFISQLPLQQHVDLKFLLFRLDFT 840
              + +    +   + +  +   L  L   Y  ++++F+  L       L+FL FRLDF 
Sbjct: 842 AEEEEENKRIREFQDSIPKMCSQLRILTHFYQGVVQQFLVLLTTSSDESLQFLSFRLDFN 891

Query: 841 EFYSQLRPHV 847
           E Y    P +
Sbjct: 902 EHYKAREPRL 891

BLAST of CSPI04G20830 vs. Swiss-Prot
Match: ALP6_SCHPO (Spindle pole body component alp6 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=alp6 PE=1 SV=2)

HSP 1 Score: 310.1 bits (793), Expect = 7.6e-83
Identity = 245/867 (28.26%), Postives = 400/867 (46.14%), Query Frame = 1

Query: 39  SLRYAIRILTS---RMTPSIAPDAAA-----IAESIKRRLATEGKSSQALT-FADLYTKF 98
           S+ Y I  + S    +  SI+PD        I   I  ++  E  +    +  ++L ++ 
Sbjct: 25  SVPYTIETIVSFFQEIIHSISPDTFQLDIDDILYKIYSKIPPEENNDALFSKLSNLVSRL 84

Query: 99  ASKTGPGSVNNKWAVLYLLKIVA----EDRKCRQTQFESSMLLPNLVASDPVLGKKSGAA 158
            S+T    ++NK  +LY L +++      R       + S+  P  + S  V     G  
Sbjct: 85  KSQT---VIHNKSQILYFLYLLSPISQSSRDVSSHLLDESISNPINIPSTEVESSNFGQT 144

Query: 159 -----PEK----EWQKGVLLVAKDPENLRDVAFKEFSNLLKEENE--------VTEEVLV 218
                PE     +W +G+       EN   ++    S+ L    E        +TE  L+
Sbjct: 145 RYDQVPENPQITDWDEGL-------ENESSISIAHDSSRLNRSTETSSVQHTLITEADLL 204

Query: 219 RDVLYACQGIDGKYVKFDNNSDGYVLSNLVKASRATRTMVRKLCEMGWLFRKVKGYISES 278
             + Y  QGI  +YV+F N     +LS  +      +  +R L E G L++++K + +  
Sbjct: 205 SSISYVLQGISTEYVQFKNELA--LLSKRIPVQYLLQ--MRALSETGLLYQELKVFSNYD 264

Query: 279 MERFPAEDIGTVGHAFC-----------AALQDELSEYYKLLAILEAQSMNPIPLVSEAA 338
                + D   V  AF            + +  EL+ +  L+A L++Q      + ++A+
Sbjct: 265 PSVSQSIDGDNVSKAFINDQSLALQSLKSVISKELTNFLALIASLDSQ------IRADAS 324

Query: 339 SSGNYLSLRRLAVWFAEPMAKVRLMAVLV-DKCRVLKGGTMAGAIHLHAQHGDPLVLEFM 398
                +++RR   W      K+R+++ +V D         +   +  +  HGDPL+ E  
Sbjct: 325 LEKPMVTIRRCIAWTQVAKLKLRILSSVVNDNMNQENKKRLIQVVSKYNVHGDPLIQELS 384

Query: 399 RRLLRRVCSPLFEMVRSWVLEGELEDIFSEFFVVGQ--------QVKAESLWREGYRLHA 458
            ++L  +  PL+EM+ +W+ +GEL D + EFFV  +        Q + + +W+  Y L  
Sbjct: 385 DKILTEITGPLYEMIENWIYKGELVDPYQEFFVKEKNGSESHDHQGQGDVVWKGKYFLDK 444

Query: 459 GMLPSFISQSLAQRILRTGKSINFLRVCCEDMGWADAATEAAVAAGTTTKRGGLGYGETD 518
            ++PSF+S+ L  +I   GKS+NF R  C D  WA    +  V          L Y +  
Sbjct: 445 ELIPSFLSEELVDKIFLIGKSLNFARYGCGDFDWAQEHYQKLVKK--------LSYRDPH 504

Query: 519 ALESLVDEAAKRIDKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGPELSEP 578
           +LE++VD+A      HL+ +M + +   DH  AIK+YLLLGQGDFV  LM+ +G  L +P
Sbjct: 505 SLETVVDKAYTESINHLVYLMEEVFHLTDHLKAIKKYLLLGQGDFVDLLMESLGNSLDQP 564

Query: 579 ANAISSFKLSGLLETAIRSSNAQYDDPDILDRLKVKMMPHGTGDRGWDVFSLEYEARVPL 638
           AN +    L+  LE+AIRSSNA Y+   +L RL  +++    G+ GWDVF+LEY+   P+
Sbjct: 565 ANTLFRHNLTASLESAIRSSNASYEPEYVLKRLDARLLELSHGETGWDVFTLEYKVDSPI 624

Query: 639 DTVFTESVMSKYLRIFNFLWKLRRVEHALIGTWKTM---KPNCITSCSLTKLH-HGVKLQ 698
           + + T     +YL+IFNFLW+L+R+E AL  +W+ +   + N   +   TK   H V   
Sbjct: 625 NVIITPYCSRQYLKIFNFLWRLKRIEFALAHSWRRVNLGERNVFRNLDYTKFEWHFVSCH 684

Query: 699 LLSTLRRCQVLWVEMNHFVTNLQYYIMFEVLEVSWSDFSNEMEAA-MDLDDLLAAHEKYL 758
           L            EM HFV  LQYYI+FEV+E+SW +    ME     LD  + AHEKY+
Sbjct: 685 L-----------AEMIHFVCQLQYYILFEVIEISWQELQLAMEKPNATLDTYIEAHEKYV 744

Query: 759 HSIFEKSLLG----EQSQTLCKSLFVLFDIILRFRSHADRLYEGIHELQCRTIESSLPSR 818
            SI  K LLG        +    L  +  +IL F    + LY     L  R I  ++P  
Sbjct: 745 TSITHKGLLGGGKSRNEDSFLHQLHDILKVILNFHDAIELLYNFSCSLSNR-IRINVPIS 804

Query: 819 DKSKKSRTTERSLETASWIADGKKALTQRAGEFLRNVEQDLAALAKEYSSLLEEFISQLP 846
             +  ++ T                           ++ +L+   +E+   L++ +  L 
Sbjct: 805 TDALAAQYTP--------------------------IKNELSNFTEEFQVRLQKLLHGLA 825

BLAST of CSPI04G20830 vs. TrEMBL
Match: A0A0A0KYU4_CUCSA (Gamma-tubulin complex component OS=Cucumis sativus GN=Csa_4G561690 PE=3 SV=1)

HSP 1 Score: 1663.7 bits (4307), Expect = 0.0e+00
Identity = 844/846 (99.76%), Postives = 845/846 (99.88%), Query Frame = 1

Query: 1   MEEDDSTKVLDLIKDLVLRLLSHNPTSNSSPTSSDFQKSLRYAIRILTSRMTPSIAPDAA 60
           MEEDDSTKVLDLIKDLVLRLLSHNPTSNSSPTSSDFQKSLRYAIRILTSRMTPSIAPDAA
Sbjct: 1   MEEDDSTKVLDLIKDLVLRLLSHNPTSNSSPTSSDFQKSLRYAIRILTSRMTPSIAPDAA 60

Query: 61  AIAESIKRRLATEGKSSQALTFADLYTKFASKTGPGSVNNKWAVLYLLKIVAEDRKCRQT 120
           AIAESIKRRLATEGKSSQALTFADLYTKFASKTGPGSVNNKWAVLYLLKIVAEDRKCRQT
Sbjct: 61  AIAESIKRRLATEGKSSQALTFADLYTKFASKTGPGSVNNKWAVLYLLKIVAEDRKCRQT 120

Query: 121 QFESSMLLPNLVASDPVLGKKSGAAPEKEWQKGVLLVAKDPENLRDVAFKEFSNLLKEEN 180
           QFESSMLLPNLVASDPVLGKKSGAAPEKEWQKGVLLVAKDPENLRDVAFKEFSNLLKEEN
Sbjct: 121 QFESSMLLPNLVASDPVLGKKSGAAPEKEWQKGVLLVAKDPENLRDVAFKEFSNLLKEEN 180

Query: 181 EVTEEVLVRDVLYACQGIDGKYVKFDNNSDGYVLSNLVKASRATRTMVRKLCEMGWLFRK 240
           EVTEEVLVRDVLYACQGIDGKYVKFDNNSDGYVLSNLVKASRATRTMVRKLCEMGWLFRK
Sbjct: 181 EVTEEVLVRDVLYACQGIDGKYVKFDNNSDGYVLSNLVKASRATRTMVRKLCEMGWLFRK 240

Query: 241 VKGYISESMERFPAEDIGTVGHAFCAALQDELSEYYKLLAILEAQSMNPIPLVSEAASSG 300
           VKGYISESMERFPAEDIGTVGHAFCAALQDELSEYYKLLAILEAQSMNPIPLVSEAASSG
Sbjct: 241 VKGYISESMERFPAEDIGTVGHAFCAALQDELSEYYKLLAILEAQSMNPIPLVSEAASSG 300

Query: 301 NYLSLRRLAVWFAEPMAKVRLMAVLVDKCRVLKGGTMAGAIHLHAQHGDPLVLEFMRRLL 360
           NYLSLRRLAVWFAEPMAK+RLMAVLVDKCRVLKGGTMAGAIHLHAQHGDPLVLEFMRRLL
Sbjct: 301 NYLSLRRLAVWFAEPMAKMRLMAVLVDKCRVLKGGTMAGAIHLHAQHGDPLVLEFMRRLL 360

Query: 361 RRVCSPLFEMVRSWVLEGELEDIFSEFFVVGQQVKAESLWREGYRLHAGMLPSFISQSLA 420
           RRVCSPLFEMVRSWVLEGELEDIFSEFFVVGQQVKAESLWREGYRLHAGMLPSFISQSLA
Sbjct: 361 RRVCSPLFEMVRSWVLEGELEDIFSEFFVVGQQVKAESLWREGYRLHAGMLPSFISQSLA 420

Query: 421 QRILRTGKSINFLRVCCEDMGWADAATEAAVAAGTTTKRGGLGYGETDALESLVDEAAKR 480
           QRILRTGKSINFLRVCCEDMGWADAATEAA AAGTTTKRGGLGYGETDALESLVDEAAKR
Sbjct: 421 QRILRTGKSINFLRVCCEDMGWADAATEAAAAAGTTTKRGGLGYGETDALESLVDEAAKR 480

Query: 481 IDKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGPELSEPANAISSFKLSGL 540
           IDKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGPELSEPANAISSFKLSGL
Sbjct: 481 IDKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGPELSEPANAISSFKLSGL 540

Query: 541 LETAIRSSNAQYDDPDILDRLKVKMMPHGTGDRGWDVFSLEYEARVPLDTVFTESVMSKY 600
           LETAIRSSNAQYDDPDILDRLKVKMMPHGTGDRGWDVFSLEYEARVPLDTVFTESVMSKY
Sbjct: 541 LETAIRSSNAQYDDPDILDRLKVKMMPHGTGDRGWDVFSLEYEARVPLDTVFTESVMSKY 600

Query: 601 LRIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLTKLHHGVKLQLLSTLRRCQVLWVEMN 660
           LRIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLTKLHHGVKLQLLSTLRRCQVLWVEMN
Sbjct: 601 LRIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLTKLHHGVKLQLLSTLRRCQVLWVEMN 660

Query: 661 HFVTNLQYYIMFEVLEVSWSDFSNEMEAAMDLDDLLAAHEKYLHSIFEKSLLGEQSQTLC 720
           HFVTNLQYYIMFEVLEVSWSDFSNEMEAAMDLDDLLAAHEKYLHSIFEKSLLGEQSQTLC
Sbjct: 661 HFVTNLQYYIMFEVLEVSWSDFSNEMEAAMDLDDLLAAHEKYLHSIFEKSLLGEQSQTLC 720

Query: 721 KSLFVLFDIILRFRSHADRLYEGIHELQCRTIESSLPSRDKSKKSRTTERSLETASWIAD 780
           KSLFVLFDIILRFRSHADRLYEGIHELQCRTIESSLPSRDKSKKSRTTERSLETASWIAD
Sbjct: 721 KSLFVLFDIILRFRSHADRLYEGIHELQCRTIESSLPSRDKSKKSRTTERSLETASWIAD 780

Query: 781 GKKALTQRAGEFLRNVEQDLAALAKEYSSLLEEFISQLPLQQHVDLKFLLFRLDFTEFYS 840
           GKKALTQRAGEFLRNVEQDLAALAKEYSSLLEEFISQLPLQQHVDLKFLLFRLDFTEFYS
Sbjct: 781 GKKALTQRAGEFLRNVEQDLAALAKEYSSLLEEFISQLPLQQHVDLKFLLFRLDFTEFYS 840

Query: 841 QLRPHV 847
           QLRPHV
Sbjct: 841 QLRPHV 846

BLAST of CSPI04G20830 vs. TrEMBL
Match: A0A061EWQ5_THECC (Gamma-tubulin complex component OS=Theobroma cacao GN=TCM_024946 PE=3 SV=1)

HSP 1 Score: 1367.1 bits (3537), Expect = 0.0e+00
Identity = 689/852 (80.87%), Postives = 760/852 (89.20%), Query Frame = 1

Query: 1   MEEDDSTKVLDLIKDLVLRLLSHNPTSNSSPTSSDFQKSLRYAIRILTSRMTPSIAPDAA 60
           MEE+D  KV DL+ +LV RLLS   + N +  S  F +SLRYA+RIL+SR+TPSI+PDA 
Sbjct: 1   MEEEDQRKVTDLVIELVRRLLSQQNSQNPNLNSPHFSQSLRYALRILSSRLTPSISPDAD 60

Query: 61  AIAESIKRRLATEGKSSQALTFADLYTKFASKTGPGSVNNKWAVLYLLKIVAEDRKCRQT 120
           AIAESIKRRLAT+G SS ALTFADLYTKFASK GPGSVNNKWAVLYLLKIV+EDRK  + 
Sbjct: 61  AIAESIKRRLATQGNSSDALTFADLYTKFASKNGPGSVNNKWAVLYLLKIVSEDRKTAKN 120

Query: 121 QFESSMLLPNLVASDPVLGKKSGAAP-----EKEWQKGVLLVAKDPENLRDVAFKEFSNL 180
             +SS  LPNL  +D  +G            EK W+ GVLLV+KDPENLR+++F+EF NL
Sbjct: 121 SMDSSFSLPNLGLNDDEMGNNLRVLNGKDNREKGWKNGVLLVSKDPENLREISFREFRNL 180

Query: 181 LKEENEVTEEVLVRDVLYACQGIDGKYVKFDNNSDGYVLSNLVKASRATRTMVRKLCEMG 240
           +KEENEV+EEVLVRDVLYACQGIDGKYVKFD+  DGY LS+LVK  RATR +VRKLCE+G
Sbjct: 181 VKEENEVSEEVLVRDVLYACQGIDGKYVKFDSTLDGYALSDLVKVPRATRIIVRKLCELG 240

Query: 241 WLFRKVKGYISESMERFPAEDIGTVGHAFCAALQDELSEYYKLLAILEAQSMNPIPLVSE 300
           WLFRKVKGYISESM+RFPAED+GTVG AFCAALQDELSEYYKLLA+LEAQSMNP+PLVSE
Sbjct: 241 WLFRKVKGYISESMDRFPAEDVGTVGQAFCAALQDELSEYYKLLAVLEAQSMNPVPLVSE 300

Query: 301 AASSGNYLSLRRLAVWFAEPMAKVRLMAVLVDKCRVLKGGTMAGAIHLHAQHGDPLVLEF 360
            ASSGNYLSLRRL+VWFAEPM K+RLMAVLVDKC+VL+GG MAGAIHLHAQHGDPLV +F
Sbjct: 301 TASSGNYLSLRRLSVWFAEPMVKMRLMAVLVDKCKVLRGGAMAGAIHLHAQHGDPLVHDF 360

Query: 361 MRRLLRRVCSPLFEMVRSWVLEGELEDIFSEFFVVGQQVKAESLWREGYRLHAGMLPSFI 420
           MRRLLRRVCSPLFEMVRSWVLEGELEDI++EFF+VGQ VKAESLWREGYRLHAGMLPSFI
Sbjct: 361 MRRLLRRVCSPLFEMVRSWVLEGELEDIYAEFFIVGQPVKAESLWREGYRLHAGMLPSFI 420

Query: 421 SQSLAQRILRTGKSINFLRVCCEDMGWADAATEAAVAAGTTTKRGGLGYGETDALESLVD 480
           SQSLAQRILRTGKSINFLRVCC+D GWADA TEAA AAGTTT+RGGLGYGETDALESLV 
Sbjct: 421 SQSLAQRILRTGKSINFLRVCCDDRGWADATTEAAAAAGTTTRRGGLGYGETDALESLVM 480

Query: 481 EAAKRIDKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGPELSEPANAISSF 540
           EAAKRIDKHLLDV++KRYKFK+HCLAIKRYLLLGQGDFVQYLMDIVGPELSEPAN ISSF
Sbjct: 481 EAAKRIDKHLLDVIYKRYKFKEHCLAIKRYLLLGQGDFVQYLMDIVGPELSEPANTISSF 540

Query: 541 KLSGLLETAIRSSNAQYDDPDILDRLKVKMMPHGTGDRGWDVFSLEYEARVPLDTVFTES 600
           KL+GLLE+AIRSSNAQYDDPDILDRL+V+MMPH TGDRGWDVFSLEY+ARVPLDTVFTES
Sbjct: 541 KLAGLLESAIRSSNAQYDDPDILDRLRVRMMPHNTGDRGWDVFSLEYDARVPLDTVFTES 600

Query: 601 VMSKYLRIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLTKLHHGVKLQLLSTLRRCQVL 660
           VM++YLRIFNFLWKLRRVEHALIG WKTMKPNCITS + TKL   VKLQLLSTLRRCQVL
Sbjct: 601 VMTRYLRIFNFLWKLRRVEHALIGAWKTMKPNCITSHAFTKLQRAVKLQLLSTLRRCQVL 660

Query: 661 WVEMNHFVTNLQYYIMFEVLEVSWSDFSNEMEAAMDLDDLLAAHEKYLHSIFEKSLLGEQ 720
           W EMNHFVTNLQYYIMFEVLEVSWS+FSNEME A DLDDLLAAHEKYLHSI EKSLLGE+
Sbjct: 661 WDEMNHFVTNLQYYIMFEVLEVSWSNFSNEMEVAKDLDDLLAAHEKYLHSIVEKSLLGER 720

Query: 721 SQTLCKSLFVLFDIILRFRSHADRLYEGIHELQCRTIESSLPSRDKSKKSR-TTERSLET 780
           SQTL KSLFVLFD+IL+FRSHADRLYEGIHELQ RT+ESS  SRDKSK SR   ++S E 
Sbjct: 721 SQTLYKSLFVLFDLILQFRSHADRLYEGIHELQSRTVESSSNSRDKSKSSRQRKDKSSEP 780

Query: 781 ASWIADGKKALTQRAGEFLRNVEQDLAALAKEYSSLLEEFISQLPLQQHVDLKFLLFRLD 840
            SWI++G+KALTQRA EFL+N+ QDL ALA EY+SLLE F++QLP+QQH+DLKFLLFRLD
Sbjct: 781 GSWISEGRKALTQRASEFLQNMGQDLDALATEYTSLLEGFLAQLPVQQHIDLKFLLFRLD 840

Query: 841 FTEFYSQLRPHV 847
           FTEFYS+  P V
Sbjct: 841 FTEFYSRQHPTV 852

BLAST of CSPI04G20830 vs. TrEMBL
Match: B9T277_RICCO (Gamma-tubulin complex component OS=Ricinus communis GN=RCOM_0413820 PE=3 SV=1)

HSP 1 Score: 1359.7 bits (3518), Expect = 0.0e+00
Identity = 685/854 (80.21%), Postives = 758/854 (88.76%), Query Frame = 1

Query: 1   MEEDDSTKVLDLIKDLVLRLLSHNP---TSNSSPTSSDFQKSLRYAIRILTSRMTPSIAP 60
           MEE+D  K++DL+K+LV RLLS NP   T NS+P S+DFQ +LRYA+RIL+SR+TPSI+P
Sbjct: 1   MEEEDQQKIIDLVKELVHRLLSQNPNFKTPNSNPNSTDFQNALRYAVRILSSRLTPSISP 60

Query: 61  DAAAIAESIKRRLATEGKSSQALTFADLYTKFASKTGPGSVNNKWAVLYLLKIVAEDRKC 120
           D+AAIAESIKRRLAT+GKSSQALTFADLY KFASK GPGSVNNKWAVLYLLKI++ED K 
Sbjct: 61  DSAAIAESIKRRLATQGKSSQALTFADLYNKFASKNGPGSVNNKWAVLYLLKIISEDEKL 120

Query: 121 RQTQFESSMLLPNLVASDPVLGKKSGA-----APEKEWQKGVLLVAKDPENLRDVAFKEF 180
            +    S+ LLP L  + P     S         +K+W  GVLLVAKDPENLR+ AFKE+
Sbjct: 121 AKNGTNSTHLLPYLALNSPDSSNDSRVNCNLKRGDKDWNNGVLLVAKDPENLREFAFKEY 180

Query: 181 SNLLKEENEVTEEVLVRDVLYACQGIDGKYVKFDNNSDGYVLSNLVKASRATRTMVRKLC 240
            NL+KEE+EVTEEVLVRDVLYACQGIDG+YVKFD N DGYVL + VK   ATR MVRKLC
Sbjct: 181 VNLVKEESEVTEEVLVRDVLYACQGIDGRYVKFDANIDGYVLMDNVKVPTATRLMVRKLC 240

Query: 241 EMGWLFRKVKGYISESMERFPAEDIGTVGHAFCAALQDELSEYYKLLAILEAQSMNPIPL 300
           E+GWLFRKVKGYISESM+RFPAED+GTVG AFCAALQDELSEYYKLLA+LEAQSMNPIPL
Sbjct: 241 ELGWLFRKVKGYISESMDRFPAEDVGTVGQAFCAALQDELSEYYKLLAVLEAQSMNPIPL 300

Query: 301 VSEAASSGNYLSLRRLAVWFAEPMAKVRLMAVLVDKCRVLKGGTMAGAIHLHAQHGDPLV 360
           +SE ASS NYLSLRRL+VWFAEPM K+RLMAVLVDKCRVL+GG MAGAIHLHAQHGDPLV
Sbjct: 301 ISEMASSSNYLSLRRLSVWFAEPMVKMRLMAVLVDKCRVLRGGAMAGAIHLHAQHGDPLV 360

Query: 361 LEFMRRLLRRVCSPLFEMVRSWVLEGELEDIFSEFFVVGQQVKAESLWREGYRLHAGMLP 420
            EFMR LL+RVCSPLFEMVRSWVLEGELED+F+EFFVVGQ VKAESLWREGYRLHAGMLP
Sbjct: 361 HEFMRNLLQRVCSPLFEMVRSWVLEGELEDLFAEFFVVGQPVKAESLWREGYRLHAGMLP 420

Query: 421 SFISQSLAQRILRTGKSINFLRVCCEDMGWADAATEAAVAAGTTTKRGGLGYGETDALES 480
           SFIS SLAQRILRTGKSINFLRVCC+D GWAD ATEAA AAGTTT+RG LGYGETDALE+
Sbjct: 421 SFISPSLAQRILRTGKSINFLRVCCDDRGWADTATEAATAAGTTTRRGSLGYGETDALET 480

Query: 481 LVDEAAKRIDKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGPELSEPANAI 540
           LV EAAKR DKHLLDVM+K YKFK+HCLAIKRYLLLGQGDFVQYLMDIVGPELSEPAN I
Sbjct: 481 LVVEAAKRTDKHLLDVMYKTYKFKEHCLAIKRYLLLGQGDFVQYLMDIVGPELSEPANTI 540

Query: 541 SSFKLSGLLETAIRSSNAQYDDPDILDRLKVKMMPHGTGDRGWDVFSLEYEARVPLDTVF 600
           SSFKL+GLLE+AIRSSNAQYDDPDILDRL+VKMMPHGTGDRGWDVFSLEY+ARVPLDTVF
Sbjct: 541 SSFKLAGLLESAIRSSNAQYDDPDILDRLRVKMMPHGTGDRGWDVFSLEYDARVPLDTVF 600

Query: 601 TESVMSKYLRIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLTKLHHGVKLQLLSTLRRC 660
           T+SVM++YLRIFNFLWKLRRVEHALIG WKTMKPNCITS +  KL   VKLQLLSTLRRC
Sbjct: 601 TKSVMARYLRIFNFLWKLRRVEHALIGAWKTMKPNCITSHAFIKLQGAVKLQLLSTLRRC 660

Query: 661 QVLWVEMNHFVTNLQYYIMFEVLEVSWSDFSNEMEAAMDLDDLLAAHEKYLHSIFEKSLL 720
           QVLW EMNHF+TNLQYYIMFEVLEVSWSDFSN+ME A DLDDLLAAHEKYLHSI EKSLL
Sbjct: 661 QVLWDEMNHFITNLQYYIMFEVLEVSWSDFSNDMEVARDLDDLLAAHEKYLHSIVEKSLL 720

Query: 721 GEQSQTLCKSLFVLFDIILRFRSHADRLYEGIHELQCRTIESSLPSRDKSKKSR-TTERS 780
           GE+SQ L KSLFVLFD+ILRFRSHADRLYEGIHELQ RT+ S+LPS+DK K  R  T++S
Sbjct: 721 GERSQLLYKSLFVLFDLILRFRSHADRLYEGIHELQARTMASTLPSQDKKKSRRQATDKS 780

Query: 781 LETASWIADGKKALTQRAGEFLRNVEQDLAALAKEYSSLLEEFISQLPLQQHVDLKFLLF 840
            E  SWI+DG+KALTQRAGEFL+N+  +L  +AKEY++LL+ F+SQLP+QQHVDLKFLLF
Sbjct: 781 SEPGSWISDGRKALTQRAGEFLQNMGHELDTVAKEYTTLLKGFLSQLPVQQHVDLKFLLF 840

Query: 841 RLDFTEFYSQLRPH 846
           RLDFTEFYS+L P+
Sbjct: 841 RLDFTEFYSRLCPN 854

BLAST of CSPI04G20830 vs. TrEMBL
Match: A0A0B0MWH5_GOSAR (Gamma-tubulin complex component OS=Gossypium arboreum GN=F383_28034 PE=3 SV=1)

HSP 1 Score: 1356.7 bits (3510), Expect = 0.0e+00
Identity = 679/846 (80.26%), Postives = 755/846 (89.24%), Query Frame = 1

Query: 1   MEEDDSTKVLDLIKDLVLRLLSHNPTSNSSPTSSDFQKSLRYAIRILTSRMTPSIAPDAA 60
           MEE+D  KV DL+ +LV RLLS     N+SP S    +SLRYA+RIL+SR+TPS++PDA 
Sbjct: 1   MEEEDQRKVTDLVIELVRRLLSQQNPQNASPNSPHLSQSLRYALRILSSRLTPSVSPDAD 60

Query: 61  AIAESIKRRLATEGKSSQALTFADLYTKFASKTGPGSVNNKWAVLYLLKIVAEDRKCRQT 120
           A+AESIKRRLAT+G SS ALTFADLYTKFASK GPGSVNNKWAVLYLLKI++EDRK   +
Sbjct: 61  AVAESIKRRLATQGNSSDALTFADLYTKFASKNGPGSVNNKWAVLYLLKIISEDRKNAIS 120

Query: 121 QFESSMLLPNLVASDPVLGK-----KSGAAPEKEWQKGVLLVAKDPENLRDVAFKEFSNL 180
             +SS+ LPNL  +D  +G       +    EK W+ GVLLV+KDPENLR+++F+EF NL
Sbjct: 121 GMDSSVFLPNLGLNDDEMGNDLRVLNAKENREKAWKNGVLLVSKDPENLREISFREFGNL 180

Query: 181 LKEENEVTEEVLVRDVLYACQGIDGKYVKFDNNSDGYVLSNLVKASRATRTMVRKLCEMG 240
           ++EENEVTEEVLVRDVLYACQGIDGKYVKFD+N DGY LS+ +K  RATRT+VRKLCE+G
Sbjct: 181 VEEENEVTEEVLVRDVLYACQGIDGKYVKFDSNLDGYALSDSIKVPRATRTIVRKLCELG 240

Query: 241 WLFRKVKGYISESMERFPAEDIGTVGHAFCAALQDELSEYYKLLAILEAQSMNPIPLVSE 300
           WLFRKVKGYISESM+RFPAED+GTVG AFCAALQDELSEYYKLLA+LEAQS NPIPLVSE
Sbjct: 241 WLFRKVKGYISESMDRFPAEDVGTVGQAFCAALQDELSEYYKLLAVLEAQSTNPIPLVSE 300

Query: 301 AASSGNYLSLRRLAVWFAEPMAKVRLMAVLVDKCRVLKGGTMAGAIHLHAQHGDPLVLEF 360
            ASSGNYLSLRRL+VWFAEPM K+RLMA+LVDKC+ L+GG MAGAIHLHAQHGDPLV +F
Sbjct: 301 NASSGNYLSLRRLSVWFAEPMVKMRLMAILVDKCKALRGGAMAGAIHLHAQHGDPLVHDF 360

Query: 361 MRRLLRRVCSPLFEMVRSWVLEGELEDIFSEFFVVGQQVKAESLWREGYRLHAGMLPSFI 420
           MRRLLRRVCSPLFEMVRSWVLEGELEDIF+EFF+VGQ VKAESLWREGYRLHAGMLP FI
Sbjct: 361 MRRLLRRVCSPLFEMVRSWVLEGELEDIFAEFFIVGQPVKAESLWREGYRLHAGMLPLFI 420

Query: 421 SQSLAQRILRTGKSINFLRVCCEDMGWADAATEAAVAAGTTTKRGGLGYGETDALESLVD 480
           SQS+AQRILRTGKSINFLRVCC+D GWADAATEA  AAG TT+RGGLGYGETDALESLV 
Sbjct: 421 SQSIAQRILRTGKSINFLRVCCDDRGWADAATEAVAAAGVTTRRGGLGYGETDALESLVM 480

Query: 481 EAAKRIDKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGPELSEPANAISSF 540
           EAAKRIDKHLLDV++KRYKFK+HCLAIKRYLLLGQGDFVQYLMDIVGPELSEPAN ISSF
Sbjct: 481 EAAKRIDKHLLDVIYKRYKFKEHCLAIKRYLLLGQGDFVQYLMDIVGPELSEPANTISSF 540

Query: 541 KLSGLLETAIRSSNAQYDDPDILDRLKVKMMPHGTGDRGWDVFSLEYEARVPLDTVFTES 600
           KL+GLLE+AIRSSNAQYDDPDILDRL+VKMMPHGTGDRGWDVFSLEY+ARVPLDTVFTES
Sbjct: 541 KLAGLLESAIRSSNAQYDDPDILDRLRVKMMPHGTGDRGWDVFSLEYDARVPLDTVFTES 600

Query: 601 VMSKYLRIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLTKLHHGVKLQLLSTLRRCQVL 660
           VM++YLRIFNFLWKLR+VEHALIG WKTMKPNC+TS   TKL H VKLQLLSTLRRCQVL
Sbjct: 601 VMTRYLRIFNFLWKLRQVEHALIGAWKTMKPNCLTSHVYTKLQHAVKLQLLSTLRRCQVL 660

Query: 661 WVEMNHFVTNLQYYIMFEVLEVSWSDFSNEMEAAMDLDDLLAAHEKYLHSIFEKSLLGEQ 720
           W EMNHFVTNLQYYIMFEVLEVSWS+FSNEME A DLDDLLAAHEKYLHSI EKSLLGE+
Sbjct: 661 WDEMNHFVTNLQYYIMFEVLEVSWSNFSNEMEVAKDLDDLLAAHEKYLHSIVEKSLLGER 720

Query: 721 SQTLCKSLFVLFDIILRFRSHADRLYEGIHELQCRTIESSLPSRDKSKKSRTTERSLETA 780
           SQTL KSLFVLFD+IL+FRS ADRLYEGIHELQ RT ESSL SRDK+K  +T ++S E  
Sbjct: 721 SQTLYKSLFVLFDLILQFRSLADRLYEGIHELQARTAESSLSSRDKNKLRQTKDKSSEPG 780

Query: 781 SWIADGKKALTQRAGEFLRNVEQDLAALAKEYSSLLEEFISQLPLQQHVDLKFLLFRLDF 840
           SWI +G+KALTQRA EFL+N+ Q+L ALA EY SLLE F++QLP+QQHVDLKFLLFRLDF
Sbjct: 781 SWIREGRKALTQRASEFLQNMGQELDALATEYKSLLEGFLTQLPVQQHVDLKFLLFRLDF 840

Query: 841 TEFYSQ 842
           TEFY++
Sbjct: 841 TEFYTR 846

BLAST of CSPI04G20830 vs. TrEMBL
Match: A0A0D2TLY7_GOSRA (Gamma-tubulin complex component OS=Gossypium raimondii GN=B456_012G099300 PE=3 SV=1)

HSP 1 Score: 1355.5 bits (3507), Expect = 0.0e+00
Identity = 677/846 (80.02%), Postives = 755/846 (89.24%), Query Frame = 1

Query: 1   MEEDDSTKVLDLIKDLVLRLLSHNPTSNSSPTSSDFQKSLRYAIRILTSRMTPSIAPDAA 60
           MEE+D  KV DL+ +LV RLLS     N+SP S  F +SLRYA+RIL+SR+TPS++PDA 
Sbjct: 1   MEEEDQRKVTDLVIELVRRLLSQQNPQNASPNSPHFSQSLRYALRILSSRLTPSVSPDAD 60

Query: 61  AIAESIKRRLATEGKSSQALTFADLYTKFASKTGPGSVNNKWAVLYLLKIVAEDRKCRQT 120
           A+AESIKRRLAT+G SS ALTFADLYTKFASK GPGSVNNKWAVLYLLKI++EDRK   +
Sbjct: 61  AVAESIKRRLATQGNSSGALTFADLYTKFASKNGPGSVNNKWAVLYLLKIISEDRKNAIS 120

Query: 121 QFESSMLLPNLVASDPVLGK-----KSGAAPEKEWQKGVLLVAKDPENLRDVAFKEFSNL 180
             +SS+ LPNL  +D  +G       +    EK W+ GVLLV+KDPENLR+++F+EF NL
Sbjct: 121 GMDSSVFLPNLGLNDDEMGNDLRVLNAKENREKAWKNGVLLVSKDPENLREISFREFGNL 180

Query: 181 LKEENEVTEEVLVRDVLYACQGIDGKYVKFDNNSDGYVLSNLVKASRATRTMVRKLCEMG 240
           ++EENEVTEEVLVRDVLYACQGIDGKYVKFD+N DGY L + +K  RATRT+VRKLCE+G
Sbjct: 181 VEEENEVTEEVLVRDVLYACQGIDGKYVKFDSNLDGYALPDSIKVPRATRTIVRKLCELG 240

Query: 241 WLFRKVKGYISESMERFPAEDIGTVGHAFCAALQDELSEYYKLLAILEAQSMNPIPLVSE 300
           WLFRKVKGYISESM+RFPAED+GTVG AFCAALQDELSEYYKLLA+LEAQS NPIPLVSE
Sbjct: 241 WLFRKVKGYISESMDRFPAEDVGTVGQAFCAALQDELSEYYKLLAVLEAQSTNPIPLVSE 300

Query: 301 AASSGNYLSLRRLAVWFAEPMAKVRLMAVLVDKCRVLKGGTMAGAIHLHAQHGDPLVLEF 360
            ASSGNYLSLRRL+VWFAEPM K+RLMAVLVDKC+ L+GG MAGAIHLHAQHGDPLV +F
Sbjct: 301 NASSGNYLSLRRLSVWFAEPMVKMRLMAVLVDKCKALRGGAMAGAIHLHAQHGDPLVHDF 360

Query: 361 MRRLLRRVCSPLFEMVRSWVLEGELEDIFSEFFVVGQQVKAESLWREGYRLHAGMLPSFI 420
           MRRLLRRVCSPLFEMVRSWVLEGELEDIF+EFF+VGQ VKAESLWREGYRLHAGMLP FI
Sbjct: 361 MRRLLRRVCSPLFEMVRSWVLEGELEDIFAEFFIVGQPVKAESLWREGYRLHAGMLPLFI 420

Query: 421 SQSLAQRILRTGKSINFLRVCCEDMGWADAATEAAVAAGTTTKRGGLGYGETDALESLVD 480
           SQS+AQRILRTGKSINFLRVCC+D GWADAATEA  AAG TT+RGGLGYGETDALESLV 
Sbjct: 421 SQSIAQRILRTGKSINFLRVCCDDRGWADAATEAVAAAGVTTRRGGLGYGETDALESLVM 480

Query: 481 EAAKRIDKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGPELSEPANAISSF 540
           EAAKRIDKHLLDV++KRYKFK+HCLAIKRYLLLGQGDFVQYLMDIVGPELSEPAN ISSF
Sbjct: 481 EAAKRIDKHLLDVIYKRYKFKEHCLAIKRYLLLGQGDFVQYLMDIVGPELSEPANTISSF 540

Query: 541 KLSGLLETAIRSSNAQYDDPDILDRLKVKMMPHGTGDRGWDVFSLEYEARVPLDTVFTES 600
           KL+GLLE+AIRSSNAQYDDPDILDRL+V+MMPHGTGDRGWDVFSLEY+ARVPLDTVFTES
Sbjct: 541 KLAGLLESAIRSSNAQYDDPDILDRLRVRMMPHGTGDRGWDVFSLEYDARVPLDTVFTES 600

Query: 601 VMSKYLRIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLTKLHHGVKLQLLSTLRRCQVL 660
           VM++YLRIFNFLWKLRRVEHALIG WKTMKPNC+TS   TKL H VKLQLLSTLRRCQVL
Sbjct: 601 VMTRYLRIFNFLWKLRRVEHALIGAWKTMKPNCLTSHVYTKLQHAVKLQLLSTLRRCQVL 660

Query: 661 WVEMNHFVTNLQYYIMFEVLEVSWSDFSNEMEAAMDLDDLLAAHEKYLHSIFEKSLLGEQ 720
           W EMNHFVTNLQYYIMFEVLEVSWS+FSNEME A DLDDLLAAHEKYL+SI EKSLLGE+
Sbjct: 661 WDEMNHFVTNLQYYIMFEVLEVSWSNFSNEMEVAKDLDDLLAAHEKYLYSIVEKSLLGER 720

Query: 721 SQTLCKSLFVLFDIILRFRSHADRLYEGIHELQCRTIESSLPSRDKSKKSRTTERSLETA 780
           SQTL KSLFVLFD+IL+FRSHADR YEGIHELQ RT ESSL S+DK+K  +T ++S E  
Sbjct: 721 SQTLYKSLFVLFDLILQFRSHADRFYEGIHELQARTAESSLSSQDKNKSRQTKDKSSEPG 780

Query: 781 SWIADGKKALTQRAGEFLRNVEQDLAALAKEYSSLLEEFISQLPLQQHVDLKFLLFRLDF 840
           SWI +G+KALTQRA EFL+N+ Q+L ALA EY SLLE F+++LP+QQHVDLKFLLFRLDF
Sbjct: 781 SWIREGRKALTQRASEFLQNMGQELDALATEYKSLLEGFLTELPVQQHVDLKFLLFRLDF 840

Query: 841 TEFYSQ 842
           TEFY++
Sbjct: 841 TEFYTR 846

BLAST of CSPI04G20830 vs. TAIR10
Match: AT5G06680.1 (AT5G06680.1 spindle pole body component 98)

HSP 1 Score: 1273.8 bits (3295), Expect = 0.0e+00
Identity = 646/844 (76.54%), Postives = 730/844 (86.49%), Query Frame = 1

Query: 1   MEEDDSTKVLDLIKDLVLRLLSHNP-TSNSSPTSSDFQKSLRYAIRILTSRMTPSIAPDA 60
           ME+DD  K  DL+++LVLRL+S NP T N  P S  F K+LRYA RIL+SR+TPS+ PDA
Sbjct: 1   MEDDDQQKAADLVQELVLRLVSQNPQTPNLDPNSPAFLKTLRYAFRILSSRLTPSVLPDA 60

Query: 61  AAIAESIKRRLATEGKSSQALTFADLYTKFASKTGPGSVNNKWAVLYLLKIVAEDRKCRQ 120
            AIAES+KRRLAT+GKSS AL FADLYTKFASKTGPGSVNNKWA++YLLKIV++DRK   
Sbjct: 61  TAIAESLKRRLATQGKSSDALAFADLYTKFASKTGPGSVNNKWALVYLLKIVSDDRKSAI 120

Query: 121 TQFESSMLLPNLVASDPVLGKKS-GAAPEKEWQKGVLLVAKDPENLRDVAFKEFSNLLKE 180
              +SS+LLPNL   D   G  S G A +K+W  GVLLV+KDPENLRD+AF+E++ L+KE
Sbjct: 121 NGLDSSVLLPNLGIGDTGNGVLSRGEAKKKDWSNGVLLVSKDPENLRDIAFREYAILVKE 180

Query: 181 ENEVTEEVLVRDVLYACQGIDGKYVKFDNNSDGYVLSNLVKASRATRTMVRKLCEMGWLF 240
           ENEVTEEVLVRDVLYA QGIDGKYVKF++  DGY +   VK  RATR MVR L E+GWLF
Sbjct: 181 ENEVTEEVLVRDVLYASQGIDGKYVKFNSEIDGYAVQESVKVPRATRIMVRMLSELGWLF 240

Query: 241 RKVKGYISESMERFPAEDIGTVGHAFCAALQDELSEYYKLLAILEAQSMNPIPLVSEAAS 300
           RKVK +I+ESM+RFPAED+GTVG AFCAALQDELS+YYKLLA+LEAQ+MNPIPLVSE+AS
Sbjct: 241 RKVKTFITESMDRFPAEDVGTVGQAFCAALQDELSDYYKLLAVLEAQAMNPIPLVSESAS 300

Query: 301 SGNYLSLRRLAVWFAEPMAKVRLMAVLVDKCRVLKGGTMAGAIHLHAQHGDPLVLEFMRR 360
           S NYLSLRRL+VWFAEPM K+RLMAVLVDKC+VL+GG MAGAIHLHAQHGDPLV +FM  
Sbjct: 301 SNNYLSLRRLSVWFAEPMVKMRLMAVLVDKCKVLRGGAMAGAIHLHAQHGDPLVHDFMMS 360

Query: 361 LLRRVCSPLFEMVRSWVLEGELEDIFSEFFVVGQQVKAESLWREGYRLHAGMLPSFISQS 420
           LLR VCSPLFEMVRSWVLEGELED F EFFVVGQ VK + LWREGY+LH  MLPSFIS S
Sbjct: 361 LLRCVCSPLFEMVRSWVLEGELEDTFGEFFVVGQPVKVDLLWREGYKLHPAMLPSFISPS 420

Query: 421 LAQRILRTGKSINFLRVCCEDMGWADAATEAAVAAGTTTKRGGLGYGETDALESLVDEAA 480
           LAQRILRTGKSINFLRVCC+D GWADAA+EAA A+GTTT+RGGLGYGETDALE LV EAA
Sbjct: 421 LAQRILRTGKSINFLRVCCDDHGWADAASEAAAASGTTTRRGGLGYGETDALEHLVTEAA 480

Query: 481 KRIDKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGPELSEPANAISSFKLS 540
           KRIDKHLLDV++KRYKFK+HCLAIKRYLLLGQGDFVQYLMDIVGP+LSEPAN ISSF+L+
Sbjct: 481 KRIDKHLLDVLYKRYKFKEHCLAIKRYLLLGQGDFVQYLMDIVGPKLSEPANNISSFELA 540

Query: 541 GLLETAIRSSNAQYDDPDILDRLKVKMMPHGTGDRGWDVFSLEYEARVPLDTVFTESVMS 600
           G LE AIR+SNAQYDD D+LDRL+VKMMPHG+GDRGWDVFSLEYEARVPLDTVFTESV+S
Sbjct: 541 GFLEAAIRASNAQYDDRDMLDRLRVKMMPHGSGDRGWDVFSLEYEARVPLDTVFTESVLS 600

Query: 601 KYLRIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLTKLHHGVKLQLLSTLRRCQVLWVE 660
           KYLR+FNFLWKL+RVEHALIG WKTMKPNCITS S  KL   VKLQLLS LRRCQVLW E
Sbjct: 601 KYLRVFNFLWKLKRVEHALIGIWKTMKPNCITSNSFVKLQSSVKLQLLSALRRCQVLWNE 660

Query: 661 MNHFVTNLQYYIMFEVLEVSWSDFSNEMEAAMDLDDLLAAHEKYLHSIFEKSLLGEQSQT 720
           MNHFVTN QYYIMFEVLEVSWS+FS EMEAA DLDDLLAAHEKYL++I  KSLLGEQSQT
Sbjct: 661 MNHFVTNFQYYIMFEVLEVSWSNFSKEMEAAKDLDDLLAAHEKYLNAIVGKSLLGEQSQT 720

Query: 721 LCKSLFVLFDIILRFRSHADRLYEGIHELQCRTIESSLPSRDKSKKSRTTERSLETASWI 780
           + +SLFVLF++ILRFRSHADRLYEGIHELQ R+ ES    R+K+K       S E  SWI
Sbjct: 721 IRESLFVLFELILRFRSHADRLYEGIHELQIRSKES---GREKNK-------SQEPGSWI 780

Query: 781 ADGKKALTQRAGEFLRNVEQDLAALAKEYSSLLEEFISQLPLQQHVDLKFLLFRLDFTEF 840
           ++G+K LTQRAGEFL+++ QD+ ++AKEY+S L+ F+S LPLQQ VDLKFL FRLDFTEF
Sbjct: 781 SEGRKGLTQRAGEFLQSMSQDMDSIAKEYTSSLDGFLSLLPLQQSVDLKFLFFRLDFTEF 834

Query: 841 YSQL 843
           YS+L
Sbjct: 841 YSRL 834

BLAST of CSPI04G20830 vs. TAIR10
Match: AT5G17410.2 (AT5G17410.2 Spc97 / Spc98 family of spindle pole body (SBP) component)

HSP 1 Score: 157.9 bits (398), Expect = 2.7e-38
Identity = 141/570 (24.74%), Postives = 257/570 (45.09%), Query Frame = 1

Query: 179 ENEVTEEVLVRDVLYACQGIDGKYV---KFDNNSDGYVLSNLVKASRATRTMVRKLCEMG 238
           +  V E +++ D+L A  GI+G+Y+   +F    D            A + + +++  + 
Sbjct: 60  DTPVQELIVIDDLLSALVGIEGRYISIKRFHGKEDSIAFQVDPSMDLALQELAKRIFPLC 119

Query: 239 WLFRKVKGYISESMERFPAEDIGTVGHAFCAALQDELSEYYKLLAILEAQSMNPIPLVSE 298
             +  +  ++ ES  +F     G V HAF AAL+  L +Y  ++A LE Q          
Sbjct: 120 EYYLLIDQFV-ESSSQFKN---GLVNHAFAAALRALLLDYQAMVAQLEHQFRL------- 179

Query: 299 AASSGNYLSLRRLAVWFAEPMAKVRLMAVLVDKCRVLKGGTMAGAIHL-----HAQHGDP 358
                  LS++ L  +    M  +R +A ++ +    K    +G ++L      A  GD 
Sbjct: 180 -----GRLSIQGLWFYCQPMMGSMRALAAVIQQAST-KQFVGSGVLNLLQSQAKAMAGDN 239

Query: 359 LVLEFMRRLLRRVCSPLFEMVRSWVLEGELEDIFSEFFVV-GQQVKAESL--------WR 418
            V   + ++     +    ++  WV EG ++D + EFF+   + +K ESL        W 
Sbjct: 240 SVRSLLEKMTECASNAYLSILERWVYEGIIDDPYGEFFIAENRSLKKESLSQDSTAKYWS 299

Query: 419 EGYRLHAGMLPSFISQSLAQRILRTGKSINFLRVCCEDMGWADAATEAAVAAGTTTKRGG 478
           + Y L    +P F++ ++A  IL TGK +N +R C  ++       +  ++  +     G
Sbjct: 300 QRYSLK-DTIPGFLA-NIAATILTTGKYLNVMRECGHNV-------QVPISERSKLTIFG 359

Query: 479 LGYGETDALESLVDEAAKRIDKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQYLMDIV 538
             +   + +++  + A+      L++++  +Y       +IK YLLL QGDF+ + MDI 
Sbjct: 360 SNHHYLECIKAAHEFASIE----LVNLIKDKYDLVGRLRSIKHYLLLDQGDFLVHFMDIA 419

Query: 539 GPELSEPANAISSFKLSGLLETAIRSSNAQYDDPD-----ILDR--LKVKMMPHGTGD-- 598
             EL++  + IS  KL  LL+ A+R++ A  D         +DR  L   +  H   D  
Sbjct: 420 REELNKKVHEISVEKLQSLLDLALRTTAAAADPRHEDLTCCVDRASLLTTLGMHKDTDSN 479

Query: 599 --------RGWDVFSLEYEARVPLDTVFTESVMSKYLRIFNFLWKLRRVEHALIGTWKTM 658
                    G + FSL Y+ + PL  V ++  +SKY  IF FL+  + VE  L G W   
Sbjct: 480 SIEDPMSITGLETFSLSYKVQWPLSIVISKKALSKYQLIFRFLFHCKHVERQLCGAW--- 539

Query: 659 KPNCITSCSLTKLHHGVKLQLL--STLRRCQVLWVEMNHFVTNLQYYIMFEVLEVSWSDF 713
                      ++H G++      + + R  +L   M  F+++L +Y+ FEVLE +W   
Sbjct: 540 -----------QIHQGIRSMNSKGTAILRSSLLCRSMLKFISSLLHYLTFEVLEPNWHVM 585

BLAST of CSPI04G20830 vs. TAIR10
Match: AT3G53760.1 (AT3G53760.1 GAMMA-TUBULIN COMPLEX PROTEIN 4)

HSP 1 Score: 108.2 bits (269), Expect = 2.5e-23
Identity = 109/477 (22.85%), Postives = 182/477 (38.16%), Query Frame = 1

Query: 332 LKGGTMAGAIHLHAQHGDPLVLEFMRRLLRRVCSPLFEMVRSWVLEGELEDIFSEFFVV- 391
           ++GG +   ++     G P +   ++RLL      ++  + +W++ G L+D   EFF+  
Sbjct: 176 IRGGQLLNVLNKRCHCGVPELRTCLQRLLWNGHQVMYNQLAAWMVYGILQDPHGEFFIKR 235

Query: 392 ----------GQQVKAESL------------WREGYRLHAGMLPSFISQSLAQRILRTGK 451
                      Q+  +E L            W  G+ +   MLP +I   L + IL  GK
Sbjct: 236 QDDGDLDHRSSQEEVSEKLARTSVHETSLTDWHSGFHISLDMLPDYIPMRLGESILFAGK 295

Query: 452 SINFLRVCCEDMGWADAATEAAVAAGTTTKRGG---------------------LGYGET 511
           +I  LR       +    +      G+   RG                      L   E 
Sbjct: 296 AIRVLRNPSPAFQFQKDKSFQQTMRGSQRIRGFMHSDFPETETELDADLTGGELLPQSEA 355

Query: 512 DALESL------------------VDEAAKRIDKHLLDVMHKRYKFKDHCLAIKRYLLLG 571
           D +E++                  VD        HL  ++  R     H  A+K Y LL 
Sbjct: 356 DKIEAMLKDLKESSEFHKRSFECTVDSVRAIAASHLWQLVVVRADLNGHLKALKDYFLLE 415

Query: 572 QGDFVQ-------YLMDIVGPELSEPANAISSFKLSGLLETA------------------ 631
           +GDF Q        LM +   + +  ++ +  F+L+     A                  
Sbjct: 416 KGDFFQCFLEESRQLMRLPPRQSTGESDLMVPFQLAATKTIAEEDKYFSRVSLRMPSFGV 475

Query: 632 -IRSSNAQYDDPDILDRLKVKM-----MPHGTGDRGWDVFSLEYEARVPLDTVFTESVMS 691
            +RSS A       + R KV +     +   T   GWD  +LEY    P+   FT+ V+S
Sbjct: 476 TVRSSQAD------MVRSKVSLTGKANLTSDTSVDGWDAIALEYSVDWPMQLFFTQEVLS 535

Query: 692 KYLRIFNFLWKLRRVEHALIGTW-KTMKPNCITSCSLTK--LHHGVKLQLLSTLRRCQVL 713
           KYL++F +L +L+R +  L  +W   M  + I S    K  L+     Q    +R    +
Sbjct: 536 KYLKVFQYLIRLKRTQMELEKSWASVMHQDHIESAQHRKDGLNGSTSQQRRQGIRPMWRV 595

BLAST of CSPI04G20830 vs. TAIR10
Match: AT3G43610.1 (AT3G43610.1 Spc97 / Spc98 family of spindle pole body (SBP) component)

HSP 1 Score: 79.3 bits (194), Expect = 1.2e-14
Identity = 72/283 (25.44%), Postives = 127/283 (44.88%), Query Frame = 1

Query: 481  IDKHLLDVMHKRYKF---------------KDHCLAIKRYLLLGQGDFVQYLMDIVGPE- 540
            IDK LL  +H +Y F               ++H LA++RY  +   D+    +  +    
Sbjct: 850  IDKCLLQEIHLQYNFVSKLAIKLLEEGFGLQEHLLALRRYHFMELADWADVFVVSLWHHK 909

Query: 541  --LSEPANAISSFKLSGLLETAIRSSNAQYD---DPDILDRLKVKMM--PHGTGDRGWDV 600
              ++E    I+  ++ G LE++I+ S+ + D   D   L + +  M   P   G R +D 
Sbjct: 910  WLVTEADKRIA--EIQGFLESSIQRSSCERDICKDRIFLYKRQGTMHIPPSTIGVRSFDF 969

Query: 601  FSLEYEARVPLDTVFTESVMSKYLRIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLTKL 660
              L Y    P+  + T   ++ Y  +F+FL +++   + L   W ++K        +  +
Sbjct: 970  LRLGYRVDWPISIILTCDALTAYADVFSFLVQVKLAAYVLTDVWCSLK-------DVRHM 1029

Query: 661  HHGVKLQLLST-LRRCQVLWV---EMNHFVTNLQYYIMFEVLEVSWSDFSNEME-AAMDL 720
             H  K ++L   LR   +L     ++NHFVT LQ Y+  E+  VSWS F + ++    D+
Sbjct: 1030 MHEKKEKILKQELRWLNILMKLRHQVNHFVTALQQYVHSELSHVSWSKFLHSLKNKVKDM 1089

Query: 721  DDLLAAHEKYLHSIFEKSLLGEQSQTLCKSLFVLFDIILRFRS 736
             DL + H  YL        L +++Q +   +  +    L FRS
Sbjct: 1090 MDLESVHMAYLSEALRICFLSDETQIISNIIENILQCALDFRS 1123

BLAST of CSPI04G20830 vs. TAIR10
Match: AT1G37010.1 (AT1G37010.1 BEST Arabidopsis thaliana protein match is: spindle pole body component 98 (TAIR:AT5G06680.1))

HSP 1 Score: 50.1 bits (118), Expect = 8.0e-06
Identity = 20/35 (57.14%), Postives = 29/35 (82.86%), Query Frame = 1

Query: 143 GAAPEKEWQKGVLLVAKDPENLRDVAFKEFSNLLK 178
           G   +K+W  GVLLV+KDPENLRD+AF++++ L+K
Sbjct: 53  GIVKKKDWSNGVLLVSKDPENLRDIAFRQYAILVK 87

BLAST of CSPI04G20830 vs. NCBI nr
Match: gi|449453900|ref|XP_004144694.1| (PREDICTED: gamma-tubulin complex component 3 [Cucumis sativus])

HSP 1 Score: 1663.7 bits (4307), Expect = 0.0e+00
Identity = 844/846 (99.76%), Postives = 845/846 (99.88%), Query Frame = 1

Query: 1   MEEDDSTKVLDLIKDLVLRLLSHNPTSNSSPTSSDFQKSLRYAIRILTSRMTPSIAPDAA 60
           MEEDDSTKVLDLIKDLVLRLLSHNPTSNSSPTSSDFQKSLRYAIRILTSRMTPSIAPDAA
Sbjct: 1   MEEDDSTKVLDLIKDLVLRLLSHNPTSNSSPTSSDFQKSLRYAIRILTSRMTPSIAPDAA 60

Query: 61  AIAESIKRRLATEGKSSQALTFADLYTKFASKTGPGSVNNKWAVLYLLKIVAEDRKCRQT 120
           AIAESIKRRLATEGKSSQALTFADLYTKFASKTGPGSVNNKWAVLYLLKIVAEDRKCRQT
Sbjct: 61  AIAESIKRRLATEGKSSQALTFADLYTKFASKTGPGSVNNKWAVLYLLKIVAEDRKCRQT 120

Query: 121 QFESSMLLPNLVASDPVLGKKSGAAPEKEWQKGVLLVAKDPENLRDVAFKEFSNLLKEEN 180
           QFESSMLLPNLVASDPVLGKKSGAAPEKEWQKGVLLVAKDPENLRDVAFKEFSNLLKEEN
Sbjct: 121 QFESSMLLPNLVASDPVLGKKSGAAPEKEWQKGVLLVAKDPENLRDVAFKEFSNLLKEEN 180

Query: 181 EVTEEVLVRDVLYACQGIDGKYVKFDNNSDGYVLSNLVKASRATRTMVRKLCEMGWLFRK 240
           EVTEEVLVRDVLYACQGIDGKYVKFDNNSDGYVLSNLVKASRATRTMVRKLCEMGWLFRK
Sbjct: 181 EVTEEVLVRDVLYACQGIDGKYVKFDNNSDGYVLSNLVKASRATRTMVRKLCEMGWLFRK 240

Query: 241 VKGYISESMERFPAEDIGTVGHAFCAALQDELSEYYKLLAILEAQSMNPIPLVSEAASSG 300
           VKGYISESMERFPAEDIGTVGHAFCAALQDELSEYYKLLAILEAQSMNPIPLVSEAASSG
Sbjct: 241 VKGYISESMERFPAEDIGTVGHAFCAALQDELSEYYKLLAILEAQSMNPIPLVSEAASSG 300

Query: 301 NYLSLRRLAVWFAEPMAKVRLMAVLVDKCRVLKGGTMAGAIHLHAQHGDPLVLEFMRRLL 360
           NYLSLRRLAVWFAEPMAK+RLMAVLVDKCRVLKGGTMAGAIHLHAQHGDPLVLEFMRRLL
Sbjct: 301 NYLSLRRLAVWFAEPMAKMRLMAVLVDKCRVLKGGTMAGAIHLHAQHGDPLVLEFMRRLL 360

Query: 361 RRVCSPLFEMVRSWVLEGELEDIFSEFFVVGQQVKAESLWREGYRLHAGMLPSFISQSLA 420
           RRVCSPLFEMVRSWVLEGELEDIFSEFFVVGQQVKAESLWREGYRLHAGMLPSFISQSLA
Sbjct: 361 RRVCSPLFEMVRSWVLEGELEDIFSEFFVVGQQVKAESLWREGYRLHAGMLPSFISQSLA 420

Query: 421 QRILRTGKSINFLRVCCEDMGWADAATEAAVAAGTTTKRGGLGYGETDALESLVDEAAKR 480
           QRILRTGKSINFLRVCCEDMGWADAATEAA AAGTTTKRGGLGYGETDALESLVDEAAKR
Sbjct: 421 QRILRTGKSINFLRVCCEDMGWADAATEAAAAAGTTTKRGGLGYGETDALESLVDEAAKR 480

Query: 481 IDKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGPELSEPANAISSFKLSGL 540
           IDKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGPELSEPANAISSFKLSGL
Sbjct: 481 IDKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGPELSEPANAISSFKLSGL 540

Query: 541 LETAIRSSNAQYDDPDILDRLKVKMMPHGTGDRGWDVFSLEYEARVPLDTVFTESVMSKY 600
           LETAIRSSNAQYDDPDILDRLKVKMMPHGTGDRGWDVFSLEYEARVPLDTVFTESVMSKY
Sbjct: 541 LETAIRSSNAQYDDPDILDRLKVKMMPHGTGDRGWDVFSLEYEARVPLDTVFTESVMSKY 600

Query: 601 LRIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLTKLHHGVKLQLLSTLRRCQVLWVEMN 660
           LRIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLTKLHHGVKLQLLSTLRRCQVLWVEMN
Sbjct: 601 LRIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLTKLHHGVKLQLLSTLRRCQVLWVEMN 660

Query: 661 HFVTNLQYYIMFEVLEVSWSDFSNEMEAAMDLDDLLAAHEKYLHSIFEKSLLGEQSQTLC 720
           HFVTNLQYYIMFEVLEVSWSDFSNEMEAAMDLDDLLAAHEKYLHSIFEKSLLGEQSQTLC
Sbjct: 661 HFVTNLQYYIMFEVLEVSWSDFSNEMEAAMDLDDLLAAHEKYLHSIFEKSLLGEQSQTLC 720

Query: 721 KSLFVLFDIILRFRSHADRLYEGIHELQCRTIESSLPSRDKSKKSRTTERSLETASWIAD 780
           KSLFVLFDIILRFRSHADRLYEGIHELQCRTIESSLPSRDKSKKSRTTERSLETASWIAD
Sbjct: 721 KSLFVLFDIILRFRSHADRLYEGIHELQCRTIESSLPSRDKSKKSRTTERSLETASWIAD 780

Query: 781 GKKALTQRAGEFLRNVEQDLAALAKEYSSLLEEFISQLPLQQHVDLKFLLFRLDFTEFYS 840
           GKKALTQRAGEFLRNVEQDLAALAKEYSSLLEEFISQLPLQQHVDLKFLLFRLDFTEFYS
Sbjct: 781 GKKALTQRAGEFLRNVEQDLAALAKEYSSLLEEFISQLPLQQHVDLKFLLFRLDFTEFYS 840

Query: 841 QLRPHV 847
           QLRPHV
Sbjct: 841 QLRPHV 846

BLAST of CSPI04G20830 vs. NCBI nr
Match: gi|659083185|ref|XP_008442226.1| (PREDICTED: gamma-tubulin complex component 3 [Cucumis melo])

HSP 1 Score: 1653.6 bits (4281), Expect = 0.0e+00
Identity = 837/846 (98.94%), Postives = 844/846 (99.76%), Query Frame = 1

Query: 1   MEEDDSTKVLDLIKDLVLRLLSHNPTSNSSPTSSDFQKSLRYAIRILTSRMTPSIAPDAA 60
           MEEDDSTKVLDLIKDLVLRLLSHNPTSNSSPTSSDFQKSLRYAIRILTSRMTPSIAPDAA
Sbjct: 1   MEEDDSTKVLDLIKDLVLRLLSHNPTSNSSPTSSDFQKSLRYAIRILTSRMTPSIAPDAA 60

Query: 61  AIAESIKRRLATEGKSSQALTFADLYTKFASKTGPGSVNNKWAVLYLLKIVAEDRKCRQT 120
           AIAESIKRRLATEGKSSQALTFADLYTKFASKTGPGSVNNKWAVLYLLKIVAEDRKCRQ+
Sbjct: 61  AIAESIKRRLATEGKSSQALTFADLYTKFASKTGPGSVNNKWAVLYLLKIVAEDRKCRQS 120

Query: 121 QFESSMLLPNLVASDPVLGKKSGAAPEKEWQKGVLLVAKDPENLRDVAFKEFSNLLKEEN 180
           QFESSMLLP+LVASDP+LGKKSGAAPEKEWQKGVLLVAKDPENLRDVAFKEFSNLLKEEN
Sbjct: 121 QFESSMLLPSLVASDPLLGKKSGAAPEKEWQKGVLLVAKDPENLRDVAFKEFSNLLKEEN 180

Query: 181 EVTEEVLVRDVLYACQGIDGKYVKFDNNSDGYVLSNLVKASRATRTMVRKLCEMGWLFRK 240
           EVTEEVLVRDVLYACQGIDGKYVKFDNNSDGYVLSNLVKASRATRTMVRKLCEMGWLFRK
Sbjct: 181 EVTEEVLVRDVLYACQGIDGKYVKFDNNSDGYVLSNLVKASRATRTMVRKLCEMGWLFRK 240

Query: 241 VKGYISESMERFPAEDIGTVGHAFCAALQDELSEYYKLLAILEAQSMNPIPLVSEAASSG 300
           VKGYISESMERFPAEDIGTVGHAFCAALQDELSEYYKLLAILEAQSMNPIPLVSEAASSG
Sbjct: 241 VKGYISESMERFPAEDIGTVGHAFCAALQDELSEYYKLLAILEAQSMNPIPLVSEAASSG 300

Query: 301 NYLSLRRLAVWFAEPMAKVRLMAVLVDKCRVLKGGTMAGAIHLHAQHGDPLVLEFMRRLL 360
           NYLSLRRLAVWFAEPMAK+RLMAVLVDKCRVLKGG MAGAIHLHAQHGDPLVLEFMRRLL
Sbjct: 301 NYLSLRRLAVWFAEPMAKMRLMAVLVDKCRVLKGGAMAGAIHLHAQHGDPLVLEFMRRLL 360

Query: 361 RRVCSPLFEMVRSWVLEGELEDIFSEFFVVGQQVKAESLWREGYRLHAGMLPSFISQSLA 420
           RRVCSPLFEMVRSWVLEGELEDIFSEFFVVGQQVKAESLWREGYRLHAGMLPSFISQSLA
Sbjct: 361 RRVCSPLFEMVRSWVLEGELEDIFSEFFVVGQQVKAESLWREGYRLHAGMLPSFISQSLA 420

Query: 421 QRILRTGKSINFLRVCCEDMGWADAATEAAVAAGTTTKRGGLGYGETDALESLVDEAAKR 480
           QRILRTGKSINFLRVCCEDMGWADAATEAA AAGTTTKRGGLGYGETDALESLVDEAAKR
Sbjct: 421 QRILRTGKSINFLRVCCEDMGWADAATEAAAAAGTTTKRGGLGYGETDALESLVDEAAKR 480

Query: 481 IDKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGPELSEPANAISSFKLSGL 540
           IDKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGP+LSEPANAISSFKLSGL
Sbjct: 481 IDKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGPDLSEPANAISSFKLSGL 540

Query: 541 LETAIRSSNAQYDDPDILDRLKVKMMPHGTGDRGWDVFSLEYEARVPLDTVFTESVMSKY 600
           LETAIRSSNAQYDDPDILDRLKVKMMPHGTGDRGWDVFSLEYEARVPLDTVFTESVMSKY
Sbjct: 541 LETAIRSSNAQYDDPDILDRLKVKMMPHGTGDRGWDVFSLEYEARVPLDTVFTESVMSKY 600

Query: 601 LRIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLTKLHHGVKLQLLSTLRRCQVLWVEMN 660
           LRIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLTKLHHGVKLQLLSTLRRCQVLWVEMN
Sbjct: 601 LRIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLTKLHHGVKLQLLSTLRRCQVLWVEMN 660

Query: 661 HFVTNLQYYIMFEVLEVSWSDFSNEMEAAMDLDDLLAAHEKYLHSIFEKSLLGEQSQTLC 720
           HFVTNLQYYIMFEVLEVSWSDFSNEMEAAMDLDDLLAAHEKYLHSIFEKSLLGEQSQTLC
Sbjct: 661 HFVTNLQYYIMFEVLEVSWSDFSNEMEAAMDLDDLLAAHEKYLHSIFEKSLLGEQSQTLC 720

Query: 721 KSLFVLFDIILRFRSHADRLYEGIHELQCRTIESSLPSRDKSKKSRTTERSLETASWIAD 780
           KSLFVLFDIILRFRSHADRLYEGIHELQCRTIESSLPSRDKSKK+RTTE+SLETASWIAD
Sbjct: 721 KSLFVLFDIILRFRSHADRLYEGIHELQCRTIESSLPSRDKSKKNRTTEKSLETASWIAD 780

Query: 781 GKKALTQRAGEFLRNVEQDLAALAKEYSSLLEEFISQLPLQQHVDLKFLLFRLDFTEFYS 840
           GKKALTQRAGEFLRNVEQDLAALAKEYSSLLEEFISQLPLQQHVDLKFLLFRLDFTEFYS
Sbjct: 781 GKKALTQRAGEFLRNVEQDLAALAKEYSSLLEEFISQLPLQQHVDLKFLLFRLDFTEFYS 840

Query: 841 QLRPHV 847
           QLRPHV
Sbjct: 841 QLRPHV 846

BLAST of CSPI04G20830 vs. NCBI nr
Match: gi|645271423|ref|XP_008240905.1| (PREDICTED: gamma-tubulin complex component 3 [Prunus mume])

HSP 1 Score: 1379.4 bits (3569), Expect = 0.0e+00
Identity = 692/852 (81.22%), Postives = 766/852 (89.91%), Query Frame = 1

Query: 1   MEEDDSTKVLDLIKDLVLRLLSHNPTSNS---SPTSSDFQKSLRYAIRILTSRMTPSIAP 60
           MEE+D  KV DLIK+LV+RL+S NP S S   +P S  FQ SLRYA R+++SR+TPS++P
Sbjct: 1   MEEEDQQKVADLIKELVIRLISQNPNSESHPPTPNSPQFQSSLRYAFRLISSRLTPSVSP 60

Query: 61  DAAAIAESIKRRLATEGKSSQALTFADLYTKFASKTGPGSVNNKWAVLYLLKIVAEDRKC 120
           DAAAIAES KRRLAT+GKSSQALTFADLYTKFASKTGPGSVNNKWAVLYLLKI++EDRK 
Sbjct: 61  DAAAIAESTKRRLATQGKSSQALTFADLYTKFASKTGPGSVNNKWAVLYLLKIISEDRKN 120

Query: 121 RQTQFESSMLLPNLVASDPVLGKKSGAA-----PEKEWQKGVLLVAKDPENLRDVAFKEF 180
            ++Q + S+LLPNL   D  LG +S         EK W  GVLLV+KDPENLR++AF+EF
Sbjct: 121 VKSQLDYSVLLPNLALHDGELGNESRVLLGKGNKEKGWNNGVLLVSKDPENLREIAFREF 180

Query: 181 SNLLKEENEVTEEVLVRDVLYACQGIDGKYVKFDNNSDGYVLSNLVKASRATRTMVRKLC 240
            NL+KEENEV+EEVLVRDVLYACQGIDGKYVK+D+ +DGYVLS+L+K  RA R MVRKLC
Sbjct: 181 VNLIKEENEVSEEVLVRDVLYACQGIDGKYVKYDSGADGYVLSDLIKVPRAIRLMVRKLC 240

Query: 241 EMGWLFRKVKGYISESMERFPAEDIGTVGHAFCAALQDELSEYYKLLAILEAQSMNPIPL 300
           E+GWLFRKVKGYISESM+ FP+ED+GTVG AFCAALQDELS+YYKLLA+LEAQSMNPIPL
Sbjct: 241 ELGWLFRKVKGYISESMDGFPSEDVGTVGQAFCAALQDELSDYYKLLAVLEAQSMNPIPL 300

Query: 301 VSEAASSGNYLSLRRLAVWFAEPMAKVRLMAVLVDKCRVLKGGTMAGAIHLHAQHGDPLV 360
           VSE ASSGNYLSLRRL+VWFAEPM K+RLMAVLVDKCRVL+GG MAGAIHLHAQHGDPLV
Sbjct: 301 VSETASSGNYLSLRRLSVWFAEPMVKMRLMAVLVDKCRVLRGGAMAGAIHLHAQHGDPLV 360

Query: 361 LEFMRRLLRRVCSPLFEMVRSWVLEGELEDIFSEFFVVGQQVKAESLWREGYRLHAGMLP 420
            EFM RLLRRVCSPLFEMVRSWVLEGELED+F+EFFVVGQ VKAESLWREGY LHAGMLP
Sbjct: 361 HEFMGRLLRRVCSPLFEMVRSWVLEGELEDVFAEFFVVGQPVKAESLWREGYLLHAGMLP 420

Query: 421 SFISQSLAQRILRTGKSINFLRVCCEDMGWADAATEAAVAAGTTTKRGGLGYGETDALES 480
           SFISQSLAQRILRTGKSINFLRVCCED GWADAATEAA AAGT+T+R GLGYGETDALES
Sbjct: 421 SFISQSLAQRILRTGKSINFLRVCCEDRGWADAATEAAAAAGTSTRRWGLGYGETDALES 480

Query: 481 LVDEAAKRIDKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGPELSEPANAI 540
           LVD AAKR+DKHLLDV++ +YKFK+HCLAIKRYLLLGQGDFVQYLMDIV PELSEPAN I
Sbjct: 481 LVDGAAKRVDKHLLDVIYNQYKFKEHCLAIKRYLLLGQGDFVQYLMDIVRPELSEPANTI 540

Query: 541 SSFKLSGLLETAIRSSNAQYDDPDILDRLKVKMMPHGTGDRGWDVFSLEYEARVPLDTVF 600
           SSF+L+GLLETA+R+SNAQYDD DILDRLKVKMMPHGTGDRGWDVFSLEY+ARVPLDTVF
Sbjct: 541 SSFQLAGLLETAVRASNAQYDDRDILDRLKVKMMPHGTGDRGWDVFSLEYDARVPLDTVF 600

Query: 601 TESVMSKYLRIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLTKLHHGVKLQLLSTLRRC 660
           TESVM+KYLRIFNFLWKLRRVEHALIG WKTMKPNCITS S  KL H VKLQLLSTLRRC
Sbjct: 601 TESVMAKYLRIFNFLWKLRRVEHALIGAWKTMKPNCITSRSFMKLQHAVKLQLLSTLRRC 660

Query: 661 QVLWVEMNHFVTNLQYYIMFEVLEVSWSDFSNEMEAAMDLDDLLAAHEKYLHSIFEKSLL 720
           QVLW EMNHFV+NLQYYIMFEVLEVSWS+F NEME A DLDDLLAAHEKYLHSI EKSLL
Sbjct: 661 QVLWDEMNHFVSNLQYYIMFEVLEVSWSNFLNEMEVAKDLDDLLAAHEKYLHSIVEKSLL 720

Query: 721 GEQSQTLCKSLFVLFDIILRFRSHADRLYEGIHELQCRTIESSLPSRDKSKKSRTTERSL 780
           GE+SQTL  SLF LFD+IL+FRSHADRL EGI+ELQ RT+ESS+PSR+KSK  +    + 
Sbjct: 721 GERSQTLYSSLFALFDLILKFRSHADRLSEGINELQARTLESSVPSRNKSKTKKQLNDTS 780

Query: 781 ETASWIADGKKALTQRAGEFLRNVEQDLAALAKEYSSLLEEFISQLPLQQHVDLKFLLFR 840
           E  SW+++G+KALTQRAGEFLRN+EQDL AL+KEYSSLLE+FIS+LP+QQHVDLKFLLFR
Sbjct: 781 EPGSWVSEGRKALTQRAGEFLRNMEQDLDALSKEYSSLLEDFISKLPMQQHVDLKFLLFR 840

Query: 841 LDFTEFYSQLRP 845
           LDFTEFYSQLRP
Sbjct: 841 LDFTEFYSQLRP 852

BLAST of CSPI04G20830 vs. NCBI nr
Match: gi|590637135|ref|XP_007029033.1| (Spindle pole body component 98 isoform 1 [Theobroma cacao])

HSP 1 Score: 1367.1 bits (3537), Expect = 0.0e+00
Identity = 689/852 (80.87%), Postives = 760/852 (89.20%), Query Frame = 1

Query: 1   MEEDDSTKVLDLIKDLVLRLLSHNPTSNSSPTSSDFQKSLRYAIRILTSRMTPSIAPDAA 60
           MEE+D  KV DL+ +LV RLLS   + N +  S  F +SLRYA+RIL+SR+TPSI+PDA 
Sbjct: 1   MEEEDQRKVTDLVIELVRRLLSQQNSQNPNLNSPHFSQSLRYALRILSSRLTPSISPDAD 60

Query: 61  AIAESIKRRLATEGKSSQALTFADLYTKFASKTGPGSVNNKWAVLYLLKIVAEDRKCRQT 120
           AIAESIKRRLAT+G SS ALTFADLYTKFASK GPGSVNNKWAVLYLLKIV+EDRK  + 
Sbjct: 61  AIAESIKRRLATQGNSSDALTFADLYTKFASKNGPGSVNNKWAVLYLLKIVSEDRKTAKN 120

Query: 121 QFESSMLLPNLVASDPVLGKKSGAAP-----EKEWQKGVLLVAKDPENLRDVAFKEFSNL 180
             +SS  LPNL  +D  +G            EK W+ GVLLV+KDPENLR+++F+EF NL
Sbjct: 121 SMDSSFSLPNLGLNDDEMGNNLRVLNGKDNREKGWKNGVLLVSKDPENLREISFREFRNL 180

Query: 181 LKEENEVTEEVLVRDVLYACQGIDGKYVKFDNNSDGYVLSNLVKASRATRTMVRKLCEMG 240
           +KEENEV+EEVLVRDVLYACQGIDGKYVKFD+  DGY LS+LVK  RATR +VRKLCE+G
Sbjct: 181 VKEENEVSEEVLVRDVLYACQGIDGKYVKFDSTLDGYALSDLVKVPRATRIIVRKLCELG 240

Query: 241 WLFRKVKGYISESMERFPAEDIGTVGHAFCAALQDELSEYYKLLAILEAQSMNPIPLVSE 300
           WLFRKVKGYISESM+RFPAED+GTVG AFCAALQDELSEYYKLLA+LEAQSMNP+PLVSE
Sbjct: 241 WLFRKVKGYISESMDRFPAEDVGTVGQAFCAALQDELSEYYKLLAVLEAQSMNPVPLVSE 300

Query: 301 AASSGNYLSLRRLAVWFAEPMAKVRLMAVLVDKCRVLKGGTMAGAIHLHAQHGDPLVLEF 360
            ASSGNYLSLRRL+VWFAEPM K+RLMAVLVDKC+VL+GG MAGAIHLHAQHGDPLV +F
Sbjct: 301 TASSGNYLSLRRLSVWFAEPMVKMRLMAVLVDKCKVLRGGAMAGAIHLHAQHGDPLVHDF 360

Query: 361 MRRLLRRVCSPLFEMVRSWVLEGELEDIFSEFFVVGQQVKAESLWREGYRLHAGMLPSFI 420
           MRRLLRRVCSPLFEMVRSWVLEGELEDI++EFF+VGQ VKAESLWREGYRLHAGMLPSFI
Sbjct: 361 MRRLLRRVCSPLFEMVRSWVLEGELEDIYAEFFIVGQPVKAESLWREGYRLHAGMLPSFI 420

Query: 421 SQSLAQRILRTGKSINFLRVCCEDMGWADAATEAAVAAGTTTKRGGLGYGETDALESLVD 480
           SQSLAQRILRTGKSINFLRVCC+D GWADA TEAA AAGTTT+RGGLGYGETDALESLV 
Sbjct: 421 SQSLAQRILRTGKSINFLRVCCDDRGWADATTEAAAAAGTTTRRGGLGYGETDALESLVM 480

Query: 481 EAAKRIDKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGPELSEPANAISSF 540
           EAAKRIDKHLLDV++KRYKFK+HCLAIKRYLLLGQGDFVQYLMDIVGPELSEPAN ISSF
Sbjct: 481 EAAKRIDKHLLDVIYKRYKFKEHCLAIKRYLLLGQGDFVQYLMDIVGPELSEPANTISSF 540

Query: 541 KLSGLLETAIRSSNAQYDDPDILDRLKVKMMPHGTGDRGWDVFSLEYEARVPLDTVFTES 600
           KL+GLLE+AIRSSNAQYDDPDILDRL+V+MMPH TGDRGWDVFSLEY+ARVPLDTVFTES
Sbjct: 541 KLAGLLESAIRSSNAQYDDPDILDRLRVRMMPHNTGDRGWDVFSLEYDARVPLDTVFTES 600

Query: 601 VMSKYLRIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLTKLHHGVKLQLLSTLRRCQVL 660
           VM++YLRIFNFLWKLRRVEHALIG WKTMKPNCITS + TKL   VKLQLLSTLRRCQVL
Sbjct: 601 VMTRYLRIFNFLWKLRRVEHALIGAWKTMKPNCITSHAFTKLQRAVKLQLLSTLRRCQVL 660

Query: 661 WVEMNHFVTNLQYYIMFEVLEVSWSDFSNEMEAAMDLDDLLAAHEKYLHSIFEKSLLGEQ 720
           W EMNHFVTNLQYYIMFEVLEVSWS+FSNEME A DLDDLLAAHEKYLHSI EKSLLGE+
Sbjct: 661 WDEMNHFVTNLQYYIMFEVLEVSWSNFSNEMEVAKDLDDLLAAHEKYLHSIVEKSLLGER 720

Query: 721 SQTLCKSLFVLFDIILRFRSHADRLYEGIHELQCRTIESSLPSRDKSKKSR-TTERSLET 780
           SQTL KSLFVLFD+IL+FRSHADRLYEGIHELQ RT+ESS  SRDKSK SR   ++S E 
Sbjct: 721 SQTLYKSLFVLFDLILQFRSHADRLYEGIHELQSRTVESSSNSRDKSKSSRQRKDKSSEP 780

Query: 781 ASWIADGKKALTQRAGEFLRNVEQDLAALAKEYSSLLEEFISQLPLQQHVDLKFLLFRLD 840
            SWI++G+KALTQRA EFL+N+ QDL ALA EY+SLLE F++QLP+QQH+DLKFLLFRLD
Sbjct: 781 GSWISEGRKALTQRASEFLQNMGQDLDALATEYTSLLEGFLAQLPVQQHIDLKFLLFRLD 840

Query: 841 FTEFYSQLRPHV 847
           FTEFYS+  P V
Sbjct: 841 FTEFYSRQHPTV 852

BLAST of CSPI04G20830 vs. NCBI nr
Match: gi|658029596|ref|XP_008350254.1| (PREDICTED: gamma-tubulin complex component 3-like [Malus domestica])

HSP 1 Score: 1361.7 bits (3523), Expect = 0.0e+00
Identity = 690/853 (80.89%), Postives = 764/853 (89.57%), Query Frame = 1

Query: 1   MEEDDSTKVLDLIKDLVLRLLSHNPTSNS---SPTSSDFQKSLRYAIRILTSRMTPSIAP 60
           MEE+D  K  DLIK+LV+RL S +P S S   +P S +FQ SLRYA R+++SR+TPS+AP
Sbjct: 1   MEEEDQQKAADLIKELVIRLRSQSPNSESHLLTPNSPEFQSSLRYAFRLISSRLTPSVAP 60

Query: 61  DAAAIAESIKRRLATEGKSSQALTFADLYTKFASKTGPGSVNNKWAVLYLLKIVAEDRKC 120
           DAAAIA+SIKRRLAT+G SSQALTFADLYTKFASKTGPGSVNNKWAV+YLLKIV+EDRK 
Sbjct: 61  DAAAIAQSIKRRLATQGXSSQALTFADLYTKFASKTGPGSVNNKWAVVYLLKIVSEDRKN 120

Query: 121 RQTQFESSMLLPNLVASDPVLGKKSGAA-----PEKEWQKGVLLVAKDPENLRDVAFKEF 180
            +TQ +SS+LLPNL  +D   GK+S         EK W  GVLLV+K PENLR++A +EF
Sbjct: 121 GKTQLDSSVLLPNLGLNDAESGKESRVLVGRENKEKGWNNGVLLVSKXPENLREIAXREF 180

Query: 181 SNLLKEENEVTEEVLVRDVLYACQGIDGKYVKFDNNSDGYVLSNLVKASRATRTMVRKLC 240
            NL+KEENEV+EEVLVRDVLYACQGIDGKYVKFD+ +DGY +S+L+K  RATR MVRKLC
Sbjct: 181 VNLIKEENEVSEEVLVRDVLYACQGIDGKYVKFDSXADGYAJSDLIKVPRATRIMVRKLC 240

Query: 241 EMGWLFRKVKGYISESMERFPAEDIGTVGHAFCAALQDELSEYYKLLAILEAQSMNPIPL 300
           E+GWLFRKVKGYISESM+ FP++++GTVG AFCAALQDELS+YYKLLA+LEAQSMNPIPL
Sbjct: 241 ELGWLFRKVKGYISESMDGFPSDNVGTVGQAFCAALQDELSDYYKLLAVLEAQSMNPIPL 300

Query: 301 VSEAASSGNYLSLRRLAVWFAEPMAKVRLMAVLVDKCRVLKGGTMAGAIHLHAQHGDPLV 360
           VSE ASS NYLSLRRL+VWFAEPM K+RLMAVLVDKCRVL+GG MAGAIHLHAQHGDPLV
Sbjct: 301 VSETASSENYLSLRRLSVWFAEPMVKMRLMAVLVDKCRVLRGGAMAGAIHLHAQHGDPLV 360

Query: 361 LEFMRRLLRRVCSPLFEMVRSWVLEGELEDIFSEFFVVGQQVKAESLWREGYRLHAGMLP 420
            EFM RLLRRVCSPLFEMVRSWVLEGELED+F+EFFVVGQ VKAESLWREGY LHAGMLP
Sbjct: 361 HEFMGRLLRRVCSPLFEMVRSWVLEGELEDVFAEFFVVGQPVKAESLWREGYMLHAGMLP 420

Query: 421 SFISQSLAQRILRTGKSINFLRVCCEDMGWADAATEAAVAAGTTTKRGGLGYGETDALES 480
           SFISQSLAQRILRTGKSINFLRVCCED GWADAATEAA AAGTTT+R GLGYGETDALES
Sbjct: 421 SFISQSLAQRILRTGKSINFLRVCCEDRGWADAATEAAAAAGTTTRRWGLGYGETDALES 480

Query: 481 LVDEAAKRIDKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGPELSEPANAI 540
           LVD AAKR+DKHLLDV++ +YKFK+HCLAIKRYLLLGQGDFVQYLMDIVGPELSEPAN I
Sbjct: 481 LVDGAAKRVDKHLLDVIYNQYKFKEHCLAIKRYLLLGQGDFVQYLMDIVGPELSEPANTI 540

Query: 541 SSFKLSGLLETAIRSSNAQYDDPDILDRLKVKMMPHGTGDRGWDVFSLEYEARVPLDTVF 600
           SSF+L+GLLETAIR+SNAQYDD DILDRLKVKMMPHGTGDRGWDVFSLEY+ARVPL TVF
Sbjct: 541 SSFQLAGLLETAIRASNAQYDDRDILDRLKVKMMPHGTGDRGWDVFSLEYDARVPLXTVF 600

Query: 601 TESVMSKYLRIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLTKLHHGVKLQLLSTLRRC 660
           TESVM+KYLRIFNFLWKLRRVEHALIG WKTMKPNCITS S  KL + VKLQLLSTLRR 
Sbjct: 601 TESVMTKYLRIFNFLWKLRRVEHALIGIWKTMKPNCITSRSFMKLPNAVKLQLLSTLRRY 660

Query: 661 QVLWVEMNHFVTNLQYYIMFEVLEVSWSDFSNEMEAAMDLDDLLAAHEKYLHSIFEKSLL 720
           QVLW EMNHFV+NLQYYIMFEVLEVSWS+F NEM+ A DLDDLLAAHEKYLHSI EKSLL
Sbjct: 661 QVLWDEMNHFVSNLQYYIMFEVLEVSWSNFLNEMDVAKDLDDLLAAHEKYLHSILEKSLL 720

Query: 721 GEQSQTLCKSLFVLFDIILRFRSHADRLYEGIHELQCRTIESSLPSRDKSK-KSRTTERS 780
           GE+SQTL  SLF LFD+IL+FRSHADRL EGI+ELQ RT+ESSLPSRDKSK K R+ +RS
Sbjct: 721 GERSQTLYDSLFALFDLILKFRSHADRLSEGINELQARTMESSLPSRDKSKAKKRSNDRS 780

Query: 781 LETASWIADGKKALTQRAGEFLRNVEQDLAALAKEYSSLLEEFISQLPLQQHVDLKFLLF 840
            E  SWI++G+KALTQ AGEFLRN+ QDL AL+KEYSSLLE+FIS+LP+QQHVDLKFLLF
Sbjct: 781 SEPGSWISEGRKALTQXAGEFLRNMGQDLDALSKEYSSLLEDFISKLPMQQHVDLKFLLF 840

Query: 841 RLDFTEFYSQLRP 845
           RLDFTEFYSQL P
Sbjct: 841 RLDFTEFYSQLHP 853