CSPI04G20730 (gene) Wild cucumber (PI 183967) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CSPI04G20730
Type	gene
Organism	Cucumis sativus (Wild cucumber (PI 183967))
Description	LINE-1 reverse transcriptase like
Location	Chr4 : 18992295 .. 18993560 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAAAGCTAAAAGGACTGAAAGCCATCCTAAAGAGTTGGAATAAGGAGACTTTTGGTAAGATTTTCTCCCAAAAACAGGTGCTGATTGATAAGATTAACTATCTTGACTCACTTGAAGAGTCAAGTTGTCTCAACGAGGAAAATGTGAAGGAAAGAGAAAATTGTAGAGGGGCTCTGCTTGATTTGATTGTGAAAGAGCAAAAGTTGTGGATTCAGAAGTCGAAGCTTCATTGGCTTAGAGAGGGGGAGGAGAACTCAAGCTTCTTCCACATATGGGTTTCGGCTCGTAAAAGTAAAAGTATTCTTTCTTCCTTGGTTAGTATCGAAGGGAAGACTCTTGTCACAGAGAAGGAAATTGTGGATGAGATCCTTAGTTTCTTTTCAAATTTATATGGCACAAGGATCTCCTCGCCGTTTATTTGTGACATTCTTAATTGGAGAGGCCTTAGCTTACAGGATTCGAGTTTACTTGAGGTTCCCTTTACCGAAAAAGAAATTAGAGAAGTTGTATTTGAGATGGGTTGTCTCAAGTCCCCTGGCCCTGATGGCTTGACTGGAGAGTTTTATAAAAAGTCATGGAACATTTTGAAGTCCGACCTCGTAAGGGTGTTCCAAGATTTTTTTAAAAACGGAATTATTAACAGAAGATGTAATGAGACTTATATTTATCTCATCCCCAAAAAGAAAGAGGCGGCCCGTGTCAGTGACTTCAGACCCATTAGCTTAATTACCTCCTTGTATAAAGTTATCTCCAAGGTGCTTCCAACAAGACTTAAAAAAGTTCTTCCTTCGATAATTAATGATTCTCAAATGGCTTTTGTGGAAGGAAGGCAAATCCTTGATGCTATTCTAACTGCTTCTGAGGCTGTTGACGAATGGTCTTTAAGAGGCAGAAAAGGTGTTCTTTTAAAGCTCGATTTGGAGAAAGCTTATGATAAGGTGGATTGGTCTTTTCTTGATATGGCCATGAAACTTAAAGGCTTTGGTAAGAGATGTAGGAAGTGGATATGGGGATGCTTGTCGACAACTAATTTTTCCATAATTGTCAACGGCAGGCCTAGAGGAAAGATTATTGCTAAAAGGGGCATTCGTCAAGGTGATCCTCTTGCCCCTTTTCTTTTTACGATAGTGGGAGATGCTCCAAGTTGCCTTATTCACTACTGTAATGAGAAAAGGAGTTTAAAAGGCTTTCATTTTGAGAACCTGTCAGAGGATTTAACCCATCTTCAGTATGCAGACGACACTCTTCTTTCTTCTTCCTAG

mRNA sequence

ATGGAAAAGCTAAAAGGACTGAAAGCCATCCTAAAGAGTTGGAATAAGGAGACTTTTGGTAAGATTTTCTCCCAAAAACAGGTGCTGATTGATAAGATTAACTATCTTGACTCACTTGAAGAGTCAAGTTGTCTCAACGAGGAAAATGTGAAGGAAAGAGAAAATTGTAGAGGGGCTCTGCTTGATTTGATTGTGAAAGAGCAAAAGTTGTGGATTCAGAAGTCGAAGCTTCATTGGCTTAGAGAGGGGGAGGAGAACTCAAGCTTCTTCCACATATGGGTTTCGGCTCGTAAAAGTAAAAGTATTCTTTCTTCCTTGGTTAGTATCGAAGGGAAGACTCTTGTCACAGAGAAGGAAATTGTGGATGAGATCCTTAGTTTCTTTTCAAATTTATATGGCACAAGGATCTCCTCGCCGTTTATTTGTGACATTCTTAATTGGAGAGGCCTTAGCTTACAGGATTCGAGTTTACTTGAGGTTCCCTTTACCGAAAAAGAAATTAGAGAAGTTGTATTTGAGATGGGTTGTCTCAAGTCCCCTGGCCCTGATGGCTTGACTGGAGAGTTTTATAAAAAGTCATGGAACATTTTGAAGTCCGACCTCGTAAGGGTGTTCCAAGATTTTTTTAAAAACGGAATTATTAACAGAAGATGTAATGAGACTTATATTTATCTCATCCCCAAAAAGAAAGAGGCGGCCCGTGTCAGTGACTTCAGACCCATTAGCTTAATTACCTCCTTGTATAAAGTTATCTCCAAGGTGCTTCCAACAAGACTTAAAAAAGTTCTTCCTTCGATAATTAATGATTCTCAAATGGCTTTTGTGGAAGGAAGGCAAATCCTTGATGCTATTCTAACTGCTTCTGAGGCTGTTGACGAATGGTCTTTAAGAGGCAGAAAAGGTGTTCTTTTAAAGCTCGATTTGGAGAAAGCTTATGATAAGGTGGATTGGTCTTTTCTTGATATGGCCATGAAACTTAAAGGCTTTGGTAAGAGATGTAGGAAGTGGATATGGGGATGCTTGTCGACAACTAATTTTTCCATAATTGTCAACGGCAGGCCTAGAGGAAAGATTATTGCTAAAAGGGGCATTCGTCAAGGTGATCCTCTTGCCCCTTTTCTTTTTACGATAGTGGGAGATGCTCCAAGTTGCCTTATTCACTACTGTAATGAGAAAAGGAGTTTAAAAGGCTTTCATTTTGAGAACCTGTCAGAGGATTTAACCCATCTTCAGTATGCAGACGACACTCTTCTTTCTTCTTCCTAG

Coding sequence (CDS)

ATGGAAAAGCTAAAAGGACTGAAAGCCATCCTAAAGAGTTGGAATAAGGAGACTTTTGGTAAGATTTTCTCCCAAAAACAGGTGCTGATTGATAAGATTAACTATCTTGACTCACTTGAAGAGTCAAGTTGTCTCAACGAGGAAAATGTGAAGGAAAGAGAAAATTGTAGAGGGGCTCTGCTTGATTTGATTGTGAAAGAGCAAAAGTTGTGGATTCAGAAGTCGAAGCTTCATTGGCTTAGAGAGGGGGAGGAGAACTCAAGCTTCTTCCACATATGGGTTTCGGCTCGTAAAAGTAAAAGTATTCTTTCTTCCTTGGTTAGTATCGAAGGGAAGACTCTTGTCACAGAGAAGGAAATTGTGGATGAGATCCTTAGTTTCTTTTCAAATTTATATGGCACAAGGATCTCCTCGCCGTTTATTTGTGACATTCTTAATTGGAGAGGCCTTAGCTTACAGGATTCGAGTTTACTTGAGGTTCCCTTTACCGAAAAAGAAATTAGAGAAGTTGTATTTGAGATGGGTTGTCTCAAGTCCCCTGGCCCTGATGGCTTGACTGGAGAGTTTTATAAAAAGTCATGGAACATTTTGAAGTCCGACCTCGTAAGGGTGTTCCAAGATTTTTTTAAAAACGGAATTATTAACAGAAGATGTAATGAGACTTATATTTATCTCATCCCCAAAAAGAAAGAGGCGGCCCGTGTCAGTGACTTCAGACCCATTAGCTTAATTACCTCCTTGTATAAAGTTATCTCCAAGGTGCTTCCAACAAGACTTAAAAAAGTTCTTCCTTCGATAATTAATGATTCTCAAATGGCTTTTGTGGAAGGAAGGCAAATCCTTGATGCTATTCTAACTGCTTCTGAGGCTGTTGACGAATGGTCTTTAAGAGGCAGAAAAGGTGTTCTTTTAAAGCTCGATTTGGAGAAAGCTTATGATAAGGTGGATTGGTCTTTTCTTGATATGGCCATGAAACTTAAAGGCTTTGGTAAGAGATGTAGGAAGTGGATATGGGGATGCTTGTCGACAACTAATTTTTCCATAATTGTCAACGGCAGGCCTAGAGGAAAGATTATTGCTAAAAGGGGCATTCGTCAAGGTGATCCTCTTGCCCCTTTTCTTTTTACGATAGTGGGAGATGCTCCAAGTTGCCTTATTCACTACTGTAATGAGAAAAGGAGTTTAAAAGGCTTTCATTTTGAGAACCTGTCAGAGGATTTAACCCATCTTCAGTATGCAGACGACACTCTTCTTTCTTCTTCCTAG

Homology

[Close]

BLAST of CSPI04G20730 vs. Swiss-Prot
Match: YTX2_XENLA (Transposon TX1 uncharacterized 149 kDa protein OS=Xenopus laevis PE=3 SV=1)

HSP 1 Score: 161.4 bits (407), Expect = 2.2e-38
Identity = 114/418 (27.27%), Postives = 202/418 (48.33%), Query Frame = 1


Query: 7   LKAILKSWNKETFGKIFSQKQVLIDKINYLD---SLEESSCLNEENVKERENCRGALLDL 66
           LK + + + K   G+  ++ + L  ++  L+   S  E   L  E ++ +E    AL ++
Sbjct: 293 LKLLCQEYTKSVSGQRNAEIEALNGEVLDLEQRLSGSEDQALQCEYLERKE----ALRNM 352

Query: 67  IVKEQKLWIQKSKLHWLREGEENSSFFHIWVSARKSKSILSSLVSIEGKTLVTEKEIVDE 126
             ++ +    +S++  L + +  S FF+     + ++  ++ L + +G  L   + I D 
Sbjct: 353 EQRQARGAFVRSRMQLLCDMDRGSRFFYALEKKKGNRKQITCLFAEDGTPLEDPEAIRDR 412

Query: 127 ILSFFSNLYGTRISSPFICDILNWRGL---SLQDSSLLEVPFTEKEIREVVFEMGCLKSP 186
             SF+ NL+     SP  C+ L W GL   S +    LE P T  E+ + +  M   KSP
Sbjct: 413 ARSFYQNLFSPDPISPDACEEL-WDGLPVVSERRKERLETPITLDELSQALRLMPHNKSP 472

Query: 187 GPDGLTGEFYKKSWNILKSDLVRVFQDFFKNGIINRRCNETYIYLIPKKKEAARVSDFRP 246
           G DGLT EF++  W+ L  D  RV  + FK G +   C    + L+PKK +   + ++RP
Sbjct: 473 GLDGLTIEFFQFFWDTLGPDFHRVLTEAFKKGELPLSCRRAVLSLLPKKGDLRLIKNWRP 532

Query: 247 ISLITSLYKVISKVLPTRLKKVLPSIINDSQMAFVEGRQILDAILTASEAVDEWSLRGRK 306
           +SL+++ YK+++K +  RLK VL  +I+  Q   V GR I D +    + +      G  
Sbjct: 533 VSLLSTDYKIVAKAISLRLKSVLAEVIHPDQSYTVPGRTIFDNVFLIRDLLHFARRTGLS 592

Query: 307 GVLLKLDLEKAYDKVDWSFLDMAMKLKGFGKRCRKWIWGCLSTTNFSIIVNGRPRGKIIA 366
              L LD EKA+D+VD  +L   ++   FG +   ++    ++    + +N      +  
Sbjct: 593 LAFLSLDQEKAFDRVDHQYLIGTLQAYSFGPQFVGYLKTMYASAECLVKINWSLTAPLAF 652

Query: 367 KRGIRQGDPLAPFLFTIVGDAPSCLIHYCNEKRSLKGFHFENLSEDLTHLQYADDTLL 419
            RG+RQG PL+  L+++  +   CL+     ++ L G   +     +    YADD +L
Sbjct: 653 GRGVRQGCPLSGQLYSLAIEPFLCLL-----RKRLTGLVLKEPDMRVVLSAYADDVIL 700

[Close]

BLAST of CSPI04G20730 vs. Swiss-Prot
Match: LIN1_NYCCO (LINE-1 reverse transcriptase homolog OS=Nycticebus coucang PE=3 SV=1)

HSP 1 Score: 139.8 bits (351), Expect = 6.8e-32
Identity = 114/422 (27.01%), Postives = 189/422 (44.79%), Query Frame = 1


Query: 3   KLKGLKAILKSWNKETFGKIFSQKQVLIDKINYLDSLEESSCLNEENVKERENCRGALLD 62
           K   L+A LK   +E    +    + L          EE S       KE    R  L +
Sbjct: 297 KFIALQAFLKKTEREEVNNLMGHLKQL--------EKEEHSNPKPSRRKEITKIRAELNE 356

Query: 63  LIVKEQKLWIQKSKLHWLREGEENSSFFHIWVSARKSKSILSSLVSIEGKTLVTEKEIVD 122
           +  K     I KSK  +  +  +           ++ KS++SS+ +   +      EI  
Sbjct: 357 IENKRIIQQINKSKSWFFEKINKIDKPLANLTRKKRVKSLISSIRNGNDEITTDPSEIQK 416

Query: 123 EILSFFSNLYGTRISSPFICD----ILNWRGLSLQDSSLLEVPFTEKEIREVVFEMGCLK 182
            +  ++  LY  +  +    D      +   LS ++  +L  P +  EI   +  +   K
Sbjct: 417 ILNEYYKKLYSHKYENLKEIDQYLEACHLPRLSQKEVEMLNRPISSSEIASTIQNLPKKK 476

Query: 183 SPGPDGLTGEFYKKSWNILKSDLVRVFQDFFKNGIINRRCNETYIYLIPKK-KEAARVSD 242
           SPGPDG T EFY+     L   L+ +FQ+  K GI+     E  I LIPK  K+  R  +
Sbjct: 477 SPGPDGFTSEFYQTFKEELVPILLNLFQNIEKEGILPNTFYEANITLIPKPGKDPTRKEN 536

Query: 243 FRPISLITSLYKVISKVLPTRLKKVLPSIINDSQMAFVEGRQILDAILTASEAVDEWS-L 302
           +RPISL+    K+++K+L  R+++ +  II+  Q+ F+ G Q    I  +   +   + L
Sbjct: 537 YRPISLMNIDAKILNKILTNRIQQHIKKIIHHDQVGFIPGSQGWFNIRKSINVIQHINKL 596

Query: 303 RGRKGVLLKLDLEKAYDKVDWSFLDMAMKLKGFGKRCRKWIWGCLSTTNFSIIVNGRPRG 362
           + +  ++L +D EKA+D +   F+   +K  G      K I    S    +II+NG    
Sbjct: 597 KNKDHMILSIDAEKAFDNIQHPFMIRTLKKIGIEGTFLKLIEAIYSKPTANIILNGVKLK 656

Query: 363 KIIAKRGIRQGDPLAPFLFTIVGDAPSCLIHYCNEKRSLKGFHFENLSEDLTHLQYADDT 419
               + G RQG PL+P LF IV +  +  I    E++++KG H    SE++    +ADD 
Sbjct: 657 SFPLRSGTRQGCPLSPLLFNIVMEVLAIAI---REEKAIKGIHIG--SEEIKLSLFADDM 705

[Close]

BLAST of CSPI04G20730 vs. Swiss-Prot
Match: LORF2_HUMAN (LINE-1 retrotransposable element ORF2 protein OS=Homo sapiens PE=1 SV=1)

HSP 1 Score: 135.2 bits (339), Expect = 1.7e-30
Identity = 88/331 (26.59%), Postives = 158/331 (47.73%), Query Frame = 1


Query: 94  VSARKSKSILSSLVSIEGKTLVTEKEIVDEILSFFSNLYGTRISS----PFICDILNWRG 153
           +  ++ K+ + ++ + +G       EI   I  ++ +LY  ++ +        D      
Sbjct: 381 IKKKREKNQIDTIKNDKGDITTDPTEIQTTIREYYKHLYANKLENLEEMDTFLDTYTLPR 440

Query: 154 LSLQDSSLLEVPFTEKEIREVVFEMGCLKSPGPDGLTGEFYKKSWNILKSDLVRVFQDFF 213
           L+ ++   L  P T  EI  ++  +   KSPGPDG T EFY++    L   L+++FQ   
Sbjct: 441 LNQEEVESLNRPITGSEIVAIINSLPTKKSPGPDGFTAEFYQRYKEELVPFLLKLFQSIE 500

Query: 214 KNGIINRRCNETYIYLIPKK-KEAARVSDFRPISLITSLYKVISKVLPTRLKKVLPSIIN 273
           K GI+     E  I LIPK  ++  +  +FRPISL+    K+++K+L  R+++ +  +I+
Sbjct: 501 KEGILPNSFYEASIILIPKPGRDTTKKENFRPISLMNIDAKILNKILANRIQQHIKKLIH 560

Query: 274 DSQMAFVEGRQILDAILTASEAVDEWS-LRGRKGVLLKLDLEKAYDKVDWSFLDMAMKLK 333
             Q+ F+ G Q    I  +   +   +  + +  V++ +D EKA+DK+   F+   +   
Sbjct: 561 HDQVGFIPGMQGWFNIRKSINVIQHINRAKDKNHVIISIDAEKAFDKIQQPFMLKTLNKL 620

Query: 334 GFGKRCRKWIWGCLSTTNFSIIVNGRPRGKIIAKRGIRQGDPLAPFLFTIVGDAPSCLIH 393
           G      K I         +II+NG+       K G RQG PL+P LF IV +    L  
Sbjct: 621 GIDGMYLKIIRAIYDKPTANIILNGQKLEAFPLKTGTRQGCPLSPLLFNIVLEV---LAR 680

Query: 394 YCNEKRSLKGFHFENLSEDLTHLQYADDTLL 419
              +++ +KG       E++    +ADD ++
Sbjct: 681 AIRQEKEIKGIQLG--KEEVKLSLFADDMIV 706

[Close]

BLAST of CSPI04G20730 vs. Swiss-Prot
Match: LORF2_MOUSE (LINE-1 retrotransposable element ORF2 protein OS=Mus musculus GN=Pol PE=1 SV=2)

HSP 1 Score: 131.3 bits (329), Expect = 2.4e-29
Identity = 92/327 (28.13%), Postives = 157/327 (48.01%), Query Frame = 1


Query: 98  KSKSILSSLVSIEGKTLVTEKEIVDEILSFFSNLYGTRISS----PFICDILNWRGLSLQ 157
           + K +++ + + +G      +EI + I SF+  LY T++ +        D      L+  
Sbjct: 392 RDKILINKIRNEKGDITTDPEEIQNTIRSFYKRLYSTKLENLDEMDKFLDRYQVPKLNQD 451

Query: 158 DSSLLEVPFTEKEIREVVFEMGCLKSPGPDGLTGEFYKKSWNILKSDLVRVFQDFFKNGI 217
               L  P + KEI  V+  +   KSPGPDG + EFY+     L   L ++F      G 
Sbjct: 452 QVDHLNSPISPKEIEAVINSLPTKKSPGPDGFSAEFYQTFKEDLIPILHKLFHKIEVEGT 511

Query: 218 INRRCNETYIYLIPK-KKEAARVSDFRPISLITSLYKVISKVLPTRLKKVLPSIINDSQM 277
           +     E  I LIPK +K+  ++ +FRPISL+    K+++K+L  R+++ + +II+  Q+
Sbjct: 512 LPNSFYEATITLIPKPQKDPTKIENFRPISLMNIDAKILNKILANRIQEHIKAIIHPDQV 571

Query: 278 AFVEGRQILDAILTASEAVDEWS-LRGRKGVLLKLDLEKAYDKVDWSFLDMAMKLKGFGK 337
            F+ G Q    I  +   +   + L+ +  +++ LD EKA+DK+   F+   ++  G   
Sbjct: 572 GFIPGMQGWFNIRKSINVIHYINKLKDKNHMIISLDAEKAFDKIQHPFMIKVLERSGIQG 631

Query: 338 RCRKWIWGCLSTTNFSIIVNGRPRGKIIAKRGIRQGDPLAPFLFTIVGDAPSCLIHYCNE 397
                I    S    +I VNG     I  K G RQG PL+P+LF IV +    L     +
Sbjct: 632 PYLNMIKAIYSKPVANIKVNGEKLEAIPLKSGTRQGCPLSPYLFNIVLEV---LARAIRQ 691

Query: 398 KRSLKGFHFENLSEDLTHLQYADDTLL 419
           ++ +KG       E++     ADD ++
Sbjct: 692 QKEIKGIQIG--KEEVKISLLADDMIV 713

[Close]

BLAST of CSPI04G20730 vs. Swiss-Prot
Match: PO22_POPJA (Retrovirus-related Pol polyprotein from type-1 retrotransposable element R2 (Fragment) OS=Popillia japonica PE=3 SV=1)

HSP 1 Score: 85.1 bits (209), Expect = 2.0e-15
Identity = 72/246 (29.27%), Postives = 119/246 (48.37%), Query Frame = 1


Query: 179 SPGPDGLTGEFYKKSWNILKSDLVRVF-QDFFKNGIINRRCNETYIYLIPKKKEAARVSD 238
           +PG DGLT +       I ++ L R F Q     G +          LIPK  +    S+
Sbjct: 22  APGSDGLTVQA------ITRTRLPRNFVQLHLLRGHVPTPWTAMRTTLIPKDGDLENPSN 81

Query: 239 FRPISLITSLYKVISKVLPTRLKKVLPSIINDSQMAFVEGRQILDAILTASEAVDEW--S 298
           +RPI++ ++L +++ ++L  RL+  +   ++ +Q  +      +D  L  S  +D +  S
Sbjct: 82  WRPITIASALQRLLHRILAKRLEAAVE--LHPAQKGYAR----IDGTLVNSLLLDTYISS 141

Query: 299 LRGRKGV--LLKLDLEKAYDKVDWSFLDMAMKLKGFGKRCRKWIWGCLSTTNFSIIVN-G 358
            R ++    ++ LD+ KA+D V  S +  A++  G  +    +I G LS +  +I V  G
Sbjct: 142 RREQRKTYNVVSLDVRKAFDTVSHSSICRALQRLGIDEGTSNYITGSLSDSTTTIRVGPG 201

Query: 359 RPRGKIIAKRGIRQGDPLAPFLFTIVGDAPSCLIHYCNEKRSLKGFHFENLSEDLTHLQY 418
               KI  +RG++QGDPL+PFLF  V D   C +      +S  G       E +  L +
Sbjct: 202 SQTRKICIRRGVKQGDPLSPFLFNAVLDELLCSL------QSTPGIGGTIGEEKIPVLAF 249

[Close]

BLAST of CSPI04G20730 vs. TrEMBL
Match: A5BV95_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_026478 PE=4 SV=1)

HSP 1 Score: 393.3 bits (1009), Expect = 3.8e-106
Identity = 190/420 (45.24%), Postives = 285/420 (67.86%), Query Frame = 1


Query: 1    MEKLKGLKAILKSWNKETFGKIFSQKQVLIDKINYLDSLEESSCLNEENVKERENCRGAL 60
            M KLK +K+ LK WN  TFG +  +K++++  ++ +D +E+   LN + V ER   R  L
Sbjct: 897  MRKLKFVKSKLKEWNIMTFGDLKERKKLILTDLSRIDLIEQEGNLNSDLVLERTLKRREL 956

Query: 61   LDLIVKEQKLWIQKSKLHWLREGEENSSFFHIWVSARKSKSILSSLVSIEGKTLVTEKEI 120
             D+++KE+  W QKS++ W++EG+ NS FFH   + R+S+  + SL+S  G+TL   ++I
Sbjct: 957  EDVLLKEEVQWRQKSRVKWIKEGDCNSKFFHRVATGRRSRKFIKSLISERGETLNNIEDI 1016

Query: 121  VDEILSFFSNLYGTRISSPFICDILNWRGLSLQDSSLLEVPFTEKEIREVVFEMGCLKSP 180
             +EI++FF NLY   +   +  + ++W  +S +    L+ PFTE+E+R  VF++   K+P
Sbjct: 1017 SEEIVNFFGNLYSKPVGESWRXEGIDWVPISGESGGWLDRPFTEEEVRRAVFQLNKEKAP 1076

Query: 181  GPDGLTGEFYKKSWNILKSDLVRVFQDFFKNGIINRRCNETYIYLIPKKKEAARVSDFRP 240
            GPDG T   Y++ W+++K DL+RVF +F  NG+IN+  N T+I L+PKK ++ ++SD+RP
Sbjct: 1077 GPDGFTIAVYQECWDVIKEDLMRVFLEFHTNGVINQSTNATFIALVPKKSQSVKISDYRP 1136

Query: 241  ISLITSLYKVISKVLPTRLKKVLPSIINDSQMAFVEGRQILDAILTASEAVDEWSLRGRK 300
            ISL+TSLYK+I+KVL  RL+KVL   I+DSQ AFVEGR ILDA+L A+E VDE    G +
Sbjct: 1137 ISLVTSLYKIIAKVLSGRLRKVLHETISDSQGAFVEGRHILDAVLIANEVVDEKRRSGEE 1196

Query: 301  GVLLKLDLEKAYDKVDWSFLDMAMKLKGFGKRCRKWIWGCLSTTNFSIIVNGRPRGKIIA 360
            G++ K+D EKAYD VDW FLD  ++ KGF ++ R WI GCLS+++F+I+VNG  +G + A
Sbjct: 1197 GIVFKIDFEKAYDHVDWGFLDHVLQRKGFSQKWRLWIRGCLSSSSFAILVNGNAKGWVKA 1256

Query: 361  KRGIRQGDPLAPFLFTIVGDAPSCLIHYCNEKRSLKGFHFENLSEDLTHLQYADDTLLSS 420
             RG+RQGDPL+PFLFT+V D  S ++    E    +GF        ++ LQ+ADDT+  S
Sbjct: 1257 SRGLRQGDPLSPFLFTLVADVLSRMLFRAEETGLTEGFSVGRDRTRVSLLQFADDTIFFS 1316

[Close]

BLAST of CSPI04G20730 vs. TrEMBL
Match: M5XUF8_PRUPE (Uncharacterized protein (Fragment) OS=Prunus persica GN=PRUPE_ppa015473mg PE=4 SV=1)

HSP 1 Score: 383.6 bits (984), Expect = 3.0e-103
Identity = 191/415 (46.02%), Postives = 270/415 (65.06%), Query Frame = 1


Query: 3   KLKGLKAILKSWNKETFGKIFSQKQVLIDKINYLDSLEESSCLNEENVKERENCRGALLD 62
           +L+ +K  +K WNKE FG + S K+    +I  LD +E    L+    KERE+    + D
Sbjct: 530 RLRTIKQKIKDWNKEVFGDLVSAKKEAEARIAALDLMEGQGGLDNILRKEREDLYFMVSD 589

Query: 63  LIVKEQKLWIQKSKLHWLREGEENSSFFHIWVSARKSKSILSSLVSIEGKTLVTEKEIVD 122
           L+ KE+  W Q+ K+ W R+G+ N+ FFH     R+ ++ +  L       +V E EI  
Sbjct: 590 LVHKEELKWRQRGKIQWARDGDSNTKFFHRIARGRRKRNFIQKLEVAGAGVVVNEWEIEL 649

Query: 123 EILSFFSNLYGTRISSPFICDILNWRGLSLQDSSLLEVPFTEKEIREVVFEMGCLKSPGP 182
           EI++FF NLY +   + +  + LNW  +S++++  LE PF E+E++  VF+ G  KSPGP
Sbjct: 650 EIINFFKNLYSSNAEAGWCLEGLNWNAISVEEAEWLERPFEEEEVKRAVFDCGIDKSPGP 709

Query: 183 DGLTGEFYKKSWNILKSDLVRVFQDFFKNGIINRRCNETYIYLIPKKKEAARVSDFRPIS 242
           DG +   ++  W  +K DL++V  DFF  GIIN   NET+I LIPKKKE+ +VSDFRPIS
Sbjct: 710 DGFSMLLFQSCWEYVKEDLMKVMADFFNCGIINAITNETFICLIPKKKESIKVSDFRPIS 769

Query: 243 LITSLYKVISKVLPTRLKKVLPSIINDSQMAFVEGRQILDAILTASEAVDEWSLRGRKGV 302
           L+TSLYK++SKVL +RL++VL S I+  Q AFV+GRQILDA L A+E V+E     + G+
Sbjct: 770 LVTSLYKMVSKVLASRLREVLGSTISSYQSAFVQGRQILDAALIANEVVEESRRLNKSGM 829

Query: 303 LLKLDLEKAYDKVDWSFLDMAMKLKGFGKRCRKWIWGCLSTTNFSIIVNGRPRGKIIAKR 362
           + K+DLEKAYD V+W F+D  +  KGFG R R WI GCL T NFS+++NGRPRGKI A R
Sbjct: 830 VFKIDLEKAYDHVEWRFVDEVLIRKGFGDRWRSWIRGCLETANFSVMINGRPRGKIRASR 889

Query: 363 GIRQGDPLAPFLFTIVGDAPSCLIHYCNEKRSLKGFHFENLSEDLTHLQYADDTL 418
           G+RQGDPL+PFLFT+V D  S ++    +     G    +   +++HLQ+ADDT+
Sbjct: 890 GLRQGDPLSPFLFTLVMDVLSRIMEKAQDTDQFHGLSPGHGMVEVSHLQFADDTI 944

[Close]

BLAST of CSPI04G20730 vs. TrEMBL
Match: A5CAA2_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_030956 PE=4 SV=1)

HSP 1 Score: 382.9 bits (982), Expect = 5.1e-103
Identity = 188/421 (44.66%), Postives = 283/421 (67.22%), Query Frame = 1


Query: 1    MEKLKGLKAILKSWNKETFGKIFSQKQVLIDKINYLDSLEESSCLNEENVKERENCRGAL 60
            M KL+ +KA LK WNK +FG++  +K+ ++  +   DSLE+   L+ E + +R   +G L
Sbjct: 1104 MRKLQFVKAKLKVWNKASFGELSKRKEDILSALVNFDSLEQEGGLSHELLAQRAIKKGEL 1163

Query: 61   LDLIVKEQKLWIQKSKLHWLREGEENSSFFHIWVSARKSKSILSSLVSIEGKTLVTEKEI 120
             +LI++E+  W QK+++ W++EG+ NS FFH   + R+++  +  L +  G+ +   + I
Sbjct: 1164 EELILREEIHWRQKARVKWVKEGDCNSKFFHKVANGRRNRKFIKELENENGQMMNNSESI 1223

Query: 121  VDEILSFFSNLYGTRISSPFICDILNWRGLSLQDSSLLEVPFTEKEIREVVFEMGCLKSP 180
             +EIL +F  LY +     +  + L+W  +S + +  LE PFTE+EI + +F+M   K+P
Sbjct: 1224 KEEILRYFEKLYTSPSGESWRVEGLDWSPISGESAVRLESPFTEEEICKAIFQMDRDKAP 1283

Query: 181  GPDGLTGEFYKKSWNILKSDLVRVFQDFFKNGIINRRCNETYIYLIPKKKEAARVSDFRP 240
            GPDG T   ++  W ++K DLV+VF +F ++GIIN+  N ++I L+PKK  + R+SDFRP
Sbjct: 1284 GPDGFTIAVFQDCWEVIKEDLVKVFTEFHRSGIINQSTNASFIVLLPKKSMSRRISDFRP 1343

Query: 241  ISLITSLYKVISKVLPTRLKKVLPSIINDSQMAFVEGRQILDAILTASEAVDEWSLRGRK 300
            ISLITSLYK+I+KVL  R+++VL   I+ +Q AFV+GRQILDA+L A+E VDE    G +
Sbjct: 1344 ISLITSLYKIIAKVLAGRIREVLHETIHSTQGAFVQGRQILDAVLIANEIVDEKRRSGEE 1403

Query: 301  GVLLKLDLEKAYDKVDWSFLDMAMKLKGFGKRCRKWIWGCLSTTNFSIIVNGRPRGKIIA 360
            GV+ K+D EKAYD V W FLD  M++KGFG R RKW+ GCLS+ +F+++VNG  +G + A
Sbjct: 1404 GVVFKIDFEKAYDHVSWDFLDHVMEMKGFGIRWRKWMRGCLSSVSFAVLVNGNAKGWVKA 1463

Query: 361  KRGIRQGDPLAPFLFTIVGDAPSCLIHYCNEKRSLKGFHFENLSEDLTHLQYADDTLLSS 420
             RG+RQGDPL+PFLFTIV D  S ++    E+  L+GF        ++HLQ+ADDT+  S
Sbjct: 1464 SRGLRQGDPLSPFLFTIVADVLSRMLLKAEERNVLEGFKVGRNRTRVSHLQFADDTIFFS 1523

Query: 421  S 422
            S
Sbjct: 1524 S 1524

[Close]

BLAST of CSPI04G20730 vs. TrEMBL
Match: A5BH71_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_013913 PE=4 SV=1)

HSP 1 Score: 381.3 bits (978), Expect = 1.5e-102
Identity = 189/421 (44.89%), Postives = 281/421 (66.75%), Query Frame = 1


Query: 1    MEKLKGLKAILKSWNKETFGKIFSQKQVLIDKINYLDSLEESSCLNEENVKERENCRGAL 60
            + KL+ +KA LK WNK +FG++  +K+ ++  +   DSLE+   L+ E + +R   +G L
Sbjct: 668  IRKLQFVKAKLKEWNKTSFGELSKRKKYILSDLANFDSLEQEGGLSHELLVQRALRKGEL 727

Query: 61   LDLIVKEQKLWIQKSKLHWLREGEENSSFFHIWVSARKSKSILSSLVSIEGKTLVTEKEI 120
             +LI++E+  W QK+++ W++EG+ NS+FFH   + R+++  +  L +  G  L   K I
Sbjct: 728  EELILREEIHWRQKARVKWVKEGDCNSNFFHKVANGRQNRKFIKELENESGLMLKDSKSI 787

Query: 121  VDEILSFFSNLYGTRISSPFICDILNWRGLSLQDSSLLEVPFTEKEIREVVFEMGCLKSP 180
             +EIL +F  LY +    P+  + L+W  +S + +S LE PFTE+EI + VF+M   K+P
Sbjct: 788  KEEILRYFEKLYVSPSGEPWRVEGLDWSPISGESASRLESPFTEEEIYKAVFQMDRDKAP 847

Query: 181  GPDGLTGEFYKKSWNILKSDLVRVFQDFFKNGIINRRCNETYIYLIPKKKEAARVSDFRP 240
            GPDG T   ++  W ++K DLVRVF +F ++GIIN+  N  +I L+PKK  + R+SDFRP
Sbjct: 848  GPDGFTIAVFQDCWKVIKEDLVRVFAEFHRSGIINQSTNAFFIVLLPKKSMSRRISDFRP 907

Query: 241  ISLITSLYKVISKVLPTRLKKVLPSIINDSQMAFVEGRQILDAILTASEAVDEWSLRGRK 300
            ISLITSLYK+I+KVL  RL+ VL   I+ +Q AFV+GRQILDA+L A+E VD+    G +
Sbjct: 908  ISLITSLYKIIAKVLAGRLRGVLHETIHSTQGAFVQGRQILDAVLIANEIVDDKRRSGEE 967

Query: 301  GVLLKLDLEKAYDKVDWSFLDMAMKLKGFGKRCRKWIWGCLSTTNFSIIVNGRPRGKIIA 360
            GV+ K+D EKAYD   W FLD  +++KGF  R RKW+ GCLS+ +F+++VNG  +G + A
Sbjct: 968  GVVFKIDFEKAYDHASWDFLDHVLEMKGFSLRWRKWMRGCLSSVSFAVLVNGNAKGWVKA 1027

Query: 361  KRGIRQGDPLAPFLFTIVGDAPSCLIHYCNEKRSLKGFHFENLSEDLTHLQYADDTLLSS 420
             RG+RQGDPL+PFLFTIV D  S ++    E+  L+GF        ++HLQ+A+DT+  S
Sbjct: 1028 SRGLRQGDPLSPFLFTIVADVLSRMLLKVEERNVLEGFRVGRNRTRVSHLQFANDTIFFS 1087

Query: 421  S 422
            S
Sbjct: 1088 S 1088

[Close]

BLAST of CSPI04G20730 vs. TrEMBL
Match: A5BQD9_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_000232 PE=4 SV=1)

HSP 1 Score: 380.9 bits (977), Expect = 1.9e-102
Identity = 191/421 (45.37%), Postives = 276/421 (65.56%), Query Frame = 1


Query: 1    MEKLKGLKAILKSWNKETFGKIFSQKQVLIDKINYLDSLEESSCLNEENVKERENCRGAL 60
            M KL+ LKA LK WNK  FG +  +K+ ++  I   DS+E+   L+ E + +R   +G L
Sbjct: 1002 MRKLQFLKAKLKEWNKNAFGDLIERKKCILLDIANFDSMEQEGGLSPELLIQRAVRKGEL 1061

Query: 61   LDLIVKEQKLWIQKSKLHWLREGEENSSFFHIWVSARKSKSILSSLVSIEGKTLVTEKEI 120
             +LI++E+  W QK+++ W++EG+ NS FFH   + R+++  +  L +  G  L     I
Sbjct: 1062 EELILREEIHWRQKARVKWVKEGDCNSKFFHKVANGRRNRKFIKVLENERGLVLDNSDSI 1121

Query: 121  VDEILSFFSNLYGTRISSPFICDILNWRGLSLQDSSLLEVPFTEKEIREVVFEMGCLKSP 180
             +EIL +F  LY +     +  + L+W  +S + +S LE PFTE+EI + +F+M   K+P
Sbjct: 1122 KEEILRYFEKLYASPSGESWRVEGLDWSPISSESASRLESPFTEEEISKAIFQMDRDKAP 1181

Query: 181  GPDGLTGEFYKKSWNILKSDLVRVFQDFFKNGIINRRCNETYIYLIPKKKEAARVSDFRP 240
            GPDG T   ++  W+++K DLVRVF +F ++GIIN+  N ++I L+PKK  A ++SD+RP
Sbjct: 1182 GPDGFTIAVFQDCWDVIKEDLVRVFDEFHRSGIINQSTNASFIVLLPKKSMAKKLSDYRP 1241

Query: 241  ISLITSLYKVISKVLPTRLKKVLPSIINDSQMAFVEGRQILDAILTASEAVDEWSLRGRK 300
            ISLITSLYK+I+KVL  RL+ VL   I+ +Q AFV+GRQILDA+L A+E VDE      +
Sbjct: 1242 ISLITSLYKIIAKVLAGRLRGVLHETIHSTQGAFVQGRQILDAVLIANEIVDEKKRSXEE 1301

Query: 301  GVLLKLDLEKAYDKVDWSFLDMAMKLKGFGKRCRKWIWGCLSTTNFSIIVNGRPRGKIIA 360
            GV+ K+D EKAYD V W FLD  M+ KGF  R RKWI GCLS+ +F+I+VNG  +G + A
Sbjct: 1302 GVVFKIDFEKAYDHVSWDFLDHVMEKKGFNPRWRKWIRGCLSSVSFAILVNGNAKGWVKA 1361

Query: 361  KRGIRQGDPLAPFLFTIVGDAPSCLIHYCNEKRSLKGFHFENLSEDLTHLQYADDTLLSS 420
             RG+RQGDPL+PFLFTIV D  S ++    E+   +GF        ++HLQ+ADDT+  S
Sbjct: 1362 SRGLRQGDPLSPFLFTIVADVMSRMLLRAEERNVFEGFRVGRNRTRVSHLQFADDTIFFS 1421

Query: 421  S 422
            S
Sbjct: 1422 S 1422

[Close]

BLAST of CSPI04G20730 vs. TAIR10
Match: AT1G43760.1 (AT1G43760.1 DNAse I-like superfamily protein)

HSP 1 Score: 114.8 bits (286), Expect = 1.3e-25
Identity = 79/262 (30.15%), Postives = 135/262 (51.53%), Query Frame = 1


Query: 2   EKLKGLKAILKSWNKETFGKIFSQKQVLIDKINYLDSL----EESSCLNEENVKERE-NC 61
           E LK  K   K  N++ FG I  + +  +D +  + S        S    E+V  ++ N 
Sbjct: 367 EHLKAAKKCCKLLNRQGFGNIQHKTKEALDSLESIQSQLLTNPSDSLFRVEHVARKKWNF 426

Query: 62  RGALLDLIVKEQKLWIQKSKLHWLREGEENSSFFHIWVSARKSKSILSSLVSIEGKTLVT 121
             A L+   +      QKS++ WL++G+ N+ FFH  + A ++K+++  L   +   +  
Sbjct: 427 FAAALESFYR------QKSRIKWLQDGDANTRFFHKVILANQAKNLIKFLRMDDDVRVEN 486

Query: 122 EKEIVDEILSFFSNLYG------TRISSPFICDILNWRGLSLQDSSLLEVPFTEKEIREV 181
             ++ + I++++++L G      T  S   I DI  +R      S L  +P ++KEI   
Sbjct: 487 VTQVKEMIVAYYTHLLGSDSDILTPDSVQRIKDIHPFRCNDTLASRLSALP-SDKEITAA 546

Query: 182 VFEMGCLKSPGPDGLTGEFYKKSWNILKSDLVRVFQDFFKNGIINRRCNETYIYLIPKKK 241
           VF M   K+PGPD  T EF+ +SW ++K   +   ++FF+ G + +R N T I LIPK  
Sbjct: 547 VFAMPRNKAPGPDSFTAEFFWESWFVVKDSTIAAVKEFFRTGHLLKRFNATAITLIPKVT 606

Query: 242 EAARVSDFRPISLITSLYKVIS 253
              ++S FRP+S  T +YK+I+
Sbjct: 607 GVDQLSMFRPVSCCTVVYKIIT 621

[Close]

BLAST of CSPI04G20730 vs. TAIR10
Match: ATMG01250.1 (ATMG01250.1 RNA-directed DNA polymerase (reverse transcriptase))

HSP 1 Score: 65.9 bits (159), Expect = 7.0e-11
Identity = 29/68 (42.65%), Postives = 40/68 (58.82%), Query Frame = 1


Query: 349 IVNGRPRGKIIAKRGIRQGDPLAPFLFTIVGDAPSCLIHYCNEKRSLKGFHFENLSEDLT 408
           I+NG P+G +   RG+RQGDPL+P+LF +  +  S L     E+  L G    N S  + 
Sbjct: 13  IINGAPQGLVTPSRGLRQGDPLSPYLFILCTEVLSGLCRRAQEQGRLPGIRVSNNSPRIN 72

Query: 409 HLQYADDT 417
           HL +ADDT
Sbjct: 73  HLLFADDT 80

[Close]

BLAST of CSPI04G20730 vs. TAIR10
Match: AT4G20520.1 (AT4G20520.1 RNA binding;RNA-directed DNA polymerases)

HSP 1 Score: 59.3 bits (142), Expect = 6.6e-09
Identity = 33/76 (43.42%), Postives = 48/76 (63.16%), Query Frame = 1


Query: 258 RLKKVLPSIINDSQMAFVEGRQILDAILTASEAVDEWSLRGRKGV----LLKLDLEKAYD 317
           RLK ++ ++I  +Q +F+ GR   D I+   EAV   S+R +KGV    LLKLDLEKAYD
Sbjct: 4   RLKPLMTNLIGPAQASFIPGRVSTDNIVFVQEAVH--SMRRKKGVKGWMLLKLDLEKAYD 63

Query: 318 KVDWSFLDMAMKLKGF 330
           ++ W +L+  +   GF
Sbjct: 64  RIRWDYLEDTLISAGF 77

[Close]

BLAST of CSPI04G20730 vs. NCBI nr
Match: gi|147784237|emb|CAN75040.1| (hypothetical protein VITISV_026478 [Vitis vinifera])

HSP 1 Score: 393.3 bits (1009), Expect = 5.4e-106
Identity = 190/420 (45.24%), Postives = 285/420 (67.86%), Query Frame = 1


Query: 1    MEKLKGLKAILKSWNKETFGKIFSQKQVLIDKINYLDSLEESSCLNEENVKERENCRGAL 60
            M KLK +K+ LK WN  TFG +  +K++++  ++ +D +E+   LN + V ER   R  L
Sbjct: 897  MRKLKFVKSKLKEWNIMTFGDLKERKKLILTDLSRIDLIEQEGNLNSDLVLERTLKRREL 956

Query: 61   LDLIVKEQKLWIQKSKLHWLREGEENSSFFHIWVSARKSKSILSSLVSIEGKTLVTEKEI 120
             D+++KE+  W QKS++ W++EG+ NS FFH   + R+S+  + SL+S  G+TL   ++I
Sbjct: 957  EDVLLKEEVQWRQKSRVKWIKEGDCNSKFFHRVATGRRSRKFIKSLISERGETLNNIEDI 1016

Query: 121  VDEILSFFSNLYGTRISSPFICDILNWRGLSLQDSSLLEVPFTEKEIREVVFEMGCLKSP 180
             +EI++FF NLY   +   +  + ++W  +S +    L+ PFTE+E+R  VF++   K+P
Sbjct: 1017 SEEIVNFFGNLYSKPVGESWRXEGIDWVPISGESGGWLDRPFTEEEVRRAVFQLNKEKAP 1076

Query: 181  GPDGLTGEFYKKSWNILKSDLVRVFQDFFKNGIINRRCNETYIYLIPKKKEAARVSDFRP 240
            GPDG T   Y++ W+++K DL+RVF +F  NG+IN+  N T+I L+PKK ++ ++SD+RP
Sbjct: 1077 GPDGFTIAVYQECWDVIKEDLMRVFLEFHTNGVINQSTNATFIALVPKKSQSVKISDYRP 1136

Query: 241  ISLITSLYKVISKVLPTRLKKVLPSIINDSQMAFVEGRQILDAILTASEAVDEWSLRGRK 300
            ISL+TSLYK+I+KVL  RL+KVL   I+DSQ AFVEGR ILDA+L A+E VDE    G +
Sbjct: 1137 ISLVTSLYKIIAKVLSGRLRKVLHETISDSQGAFVEGRHILDAVLIANEVVDEKRRSGEE 1196

Query: 301  GVLLKLDLEKAYDKVDWSFLDMAMKLKGFGKRCRKWIWGCLSTTNFSIIVNGRPRGKIIA 360
            G++ K+D EKAYD VDW FLD  ++ KGF ++ R WI GCLS+++F+I+VNG  +G + A
Sbjct: 1197 GIVFKIDFEKAYDHVDWGFLDHVLQRKGFSQKWRLWIRGCLSSSSFAILVNGNAKGWVKA 1256

Query: 361  KRGIRQGDPLAPFLFTIVGDAPSCLIHYCNEKRSLKGFHFENLSEDLTHLQYADDTLLSS 420
             RG+RQGDPL+PFLFT+V D  S ++    E    +GF        ++ LQ+ADDT+  S
Sbjct: 1257 SRGLRQGDPLSPFLFTLVADVLSRMLFRAEETGLTEGFSVGRDRTRVSLLQFADDTIFFS 1316

[Close]

BLAST of CSPI04G20730 vs. NCBI nr
Match: gi|596221970|ref|XP_007224079.1| (hypothetical protein PRUPE_ppa015473mg, partial [Prunus persica])

HSP 1 Score: 383.6 bits (984), Expect = 4.3e-103
Identity = 191/415 (46.02%), Postives = 270/415 (65.06%), Query Frame = 1


Query: 3   KLKGLKAILKSWNKETFGKIFSQKQVLIDKINYLDSLEESSCLNEENVKERENCRGALLD 62
           +L+ +K  +K WNKE FG + S K+    +I  LD +E    L+    KERE+    + D
Sbjct: 530 RLRTIKQKIKDWNKEVFGDLVSAKKEAEARIAALDLMEGQGGLDNILRKEREDLYFMVSD 589

Query: 63  LIVKEQKLWIQKSKLHWLREGEENSSFFHIWVSARKSKSILSSLVSIEGKTLVTEKEIVD 122
           L+ KE+  W Q+ K+ W R+G+ N+ FFH     R+ ++ +  L       +V E EI  
Sbjct: 590 LVHKEELKWRQRGKIQWARDGDSNTKFFHRIARGRRKRNFIQKLEVAGAGVVVNEWEIEL 649

Query: 123 EILSFFSNLYGTRISSPFICDILNWRGLSLQDSSLLEVPFTEKEIREVVFEMGCLKSPGP 182
           EI++FF NLY +   + +  + LNW  +S++++  LE PF E+E++  VF+ G  KSPGP
Sbjct: 650 EIINFFKNLYSSNAEAGWCLEGLNWNAISVEEAEWLERPFEEEEVKRAVFDCGIDKSPGP 709

Query: 183 DGLTGEFYKKSWNILKSDLVRVFQDFFKNGIINRRCNETYIYLIPKKKEAARVSDFRPIS 242
           DG +   ++  W  +K DL++V  DFF  GIIN   NET+I LIPKKKE+ +VSDFRPIS
Sbjct: 710 DGFSMLLFQSCWEYVKEDLMKVMADFFNCGIINAITNETFICLIPKKKESIKVSDFRPIS 769

Query: 243 LITSLYKVISKVLPTRLKKVLPSIINDSQMAFVEGRQILDAILTASEAVDEWSLRGRKGV 302
           L+TSLYK++SKVL +RL++VL S I+  Q AFV+GRQILDA L A+E V+E     + G+
Sbjct: 770 LVTSLYKMVSKVLASRLREVLGSTISSYQSAFVQGRQILDAALIANEVVEESRRLNKSGM 829

Query: 303 LLKLDLEKAYDKVDWSFLDMAMKLKGFGKRCRKWIWGCLSTTNFSIIVNGRPRGKIIAKR 362
           + K+DLEKAYD V+W F+D  +  KGFG R R WI GCL T NFS+++NGRPRGKI A R
Sbjct: 830 VFKIDLEKAYDHVEWRFVDEVLIRKGFGDRWRSWIRGCLETANFSVMINGRPRGKIRASR 889

Query: 363 GIRQGDPLAPFLFTIVGDAPSCLIHYCNEKRSLKGFHFENLSEDLTHLQYADDTL 418
           G+RQGDPL+PFLFT+V D  S ++    +     G    +   +++HLQ+ADDT+
Sbjct: 890 GLRQGDPLSPFLFTLVMDVLSRIMEKAQDTDQFHGLSPGHGMVEVSHLQFADDTI 944

[Close]

BLAST of CSPI04G20730 vs. NCBI nr
Match: gi|147803328|emb|CAN68838.1| (hypothetical protein VITISV_030956 [Vitis vinifera])

HSP 1 Score: 382.9 bits (982), Expect = 7.4e-103
Identity = 188/421 (44.66%), Postives = 283/421 (67.22%), Query Frame = 1


Query: 1    MEKLKGLKAILKSWNKETFGKIFSQKQVLIDKINYLDSLEESSCLNEENVKERENCRGAL 60
            M KL+ +KA LK WNK +FG++  +K+ ++  +   DSLE+   L+ E + +R   +G L
Sbjct: 1104 MRKLQFVKAKLKVWNKASFGELSKRKEDILSALVNFDSLEQEGGLSHELLAQRAIKKGEL 1163

Query: 61   LDLIVKEQKLWIQKSKLHWLREGEENSSFFHIWVSARKSKSILSSLVSIEGKTLVTEKEI 120
             +LI++E+  W QK+++ W++EG+ NS FFH   + R+++  +  L +  G+ +   + I
Sbjct: 1164 EELILREEIHWRQKARVKWVKEGDCNSKFFHKVANGRRNRKFIKELENENGQMMNNSESI 1223

Query: 121  VDEILSFFSNLYGTRISSPFICDILNWRGLSLQDSSLLEVPFTEKEIREVVFEMGCLKSP 180
             +EIL +F  LY +     +  + L+W  +S + +  LE PFTE+EI + +F+M   K+P
Sbjct: 1224 KEEILRYFEKLYTSPSGESWRVEGLDWSPISGESAVRLESPFTEEEICKAIFQMDRDKAP 1283

Query: 181  GPDGLTGEFYKKSWNILKSDLVRVFQDFFKNGIINRRCNETYIYLIPKKKEAARVSDFRP 240
            GPDG T   ++  W ++K DLV+VF +F ++GIIN+  N ++I L+PKK  + R+SDFRP
Sbjct: 1284 GPDGFTIAVFQDCWEVIKEDLVKVFTEFHRSGIINQSTNASFIVLLPKKSMSRRISDFRP 1343

Query: 241  ISLITSLYKVISKVLPTRLKKVLPSIINDSQMAFVEGRQILDAILTASEAVDEWSLRGRK 300
            ISLITSLYK+I+KVL  R+++VL   I+ +Q AFV+GRQILDA+L A+E VDE    G +
Sbjct: 1344 ISLITSLYKIIAKVLAGRIREVLHETIHSTQGAFVQGRQILDAVLIANEIVDEKRRSGEE 1403

Query: 301  GVLLKLDLEKAYDKVDWSFLDMAMKLKGFGKRCRKWIWGCLSTTNFSIIVNGRPRGKIIA 360
            GV+ K+D EKAYD V W FLD  M++KGFG R RKW+ GCLS+ +F+++VNG  +G + A
Sbjct: 1404 GVVFKIDFEKAYDHVSWDFLDHVMEMKGFGIRWRKWMRGCLSSVSFAVLVNGNAKGWVKA 1463

Query: 361  KRGIRQGDPLAPFLFTIVGDAPSCLIHYCNEKRSLKGFHFENLSEDLTHLQYADDTLLSS 420
             RG+RQGDPL+PFLFTIV D  S ++    E+  L+GF        ++HLQ+ADDT+  S
Sbjct: 1464 SRGLRQGDPLSPFLFTIVADVLSRMLLKAEERNVLEGFKVGRNRTRVSHLQFADDTIFFS 1523

Query: 421  S 422
            S
Sbjct: 1524 S 1524

[Close]

BLAST of CSPI04G20730 vs. NCBI nr
Match: gi|147815144|emb|CAN67932.1| (hypothetical protein VITISV_013913 [Vitis vinifera])

HSP 1 Score: 381.3 bits (978), Expect = 2.1e-102
Identity = 189/421 (44.89%), Postives = 281/421 (66.75%), Query Frame = 1


Query: 1    MEKLKGLKAILKSWNKETFGKIFSQKQVLIDKINYLDSLEESSCLNEENVKERENCRGAL 60
            + KL+ +KA LK WNK +FG++  +K+ ++  +   DSLE+   L+ E + +R   +G L
Sbjct: 668  IRKLQFVKAKLKEWNKTSFGELSKRKKYILSDLANFDSLEQEGGLSHELLVQRALRKGEL 727

Query: 61   LDLIVKEQKLWIQKSKLHWLREGEENSSFFHIWVSARKSKSILSSLVSIEGKTLVTEKEI 120
             +LI++E+  W QK+++ W++EG+ NS+FFH   + R+++  +  L +  G  L   K I
Sbjct: 728  EELILREEIHWRQKARVKWVKEGDCNSNFFHKVANGRQNRKFIKELENESGLMLKDSKSI 787

Query: 121  VDEILSFFSNLYGTRISSPFICDILNWRGLSLQDSSLLEVPFTEKEIREVVFEMGCLKSP 180
             +EIL +F  LY +    P+  + L+W  +S + +S LE PFTE+EI + VF+M   K+P
Sbjct: 788  KEEILRYFEKLYVSPSGEPWRVEGLDWSPISGESASRLESPFTEEEIYKAVFQMDRDKAP 847

Query: 181  GPDGLTGEFYKKSWNILKSDLVRVFQDFFKNGIINRRCNETYIYLIPKKKEAARVSDFRP 240
            GPDG T   ++  W ++K DLVRVF +F ++GIIN+  N  +I L+PKK  + R+SDFRP
Sbjct: 848  GPDGFTIAVFQDCWKVIKEDLVRVFAEFHRSGIINQSTNAFFIVLLPKKSMSRRISDFRP 907

Query: 241  ISLITSLYKVISKVLPTRLKKVLPSIINDSQMAFVEGRQILDAILTASEAVDEWSLRGRK 300
            ISLITSLYK+I+KVL  RL+ VL   I+ +Q AFV+GRQILDA+L A+E VD+    G +
Sbjct: 908  ISLITSLYKIIAKVLAGRLRGVLHETIHSTQGAFVQGRQILDAVLIANEIVDDKRRSGEE 967

Query: 301  GVLLKLDLEKAYDKVDWSFLDMAMKLKGFGKRCRKWIWGCLSTTNFSIIVNGRPRGKIIA 360
            GV+ K+D EKAYD   W FLD  +++KGF  R RKW+ GCLS+ +F+++VNG  +G + A
Sbjct: 968  GVVFKIDFEKAYDHASWDFLDHVLEMKGFSLRWRKWMRGCLSSVSFAVLVNGNAKGWVKA 1027

Query: 361  KRGIRQGDPLAPFLFTIVGDAPSCLIHYCNEKRSLKGFHFENLSEDLTHLQYADDTLLSS 420
             RG+RQGDPL+PFLFTIV D  S ++    E+  L+GF        ++HLQ+A+DT+  S
Sbjct: 1028 SRGLRQGDPLSPFLFTIVADVLSRMLLKVEERNVLEGFRVGRNRTRVSHLQFANDTIFFS 1087

Query: 421  S 422
            S
Sbjct: 1088 S 1088

[Close]

BLAST of CSPI04G20730 vs. NCBI nr
Match: gi|147857332|emb|CAN79190.1| (hypothetical protein VITISV_000232 [Vitis vinifera])

HSP 1 Score: 380.9 bits (977), Expect = 2.8e-102
Identity = 191/421 (45.37%), Postives = 276/421 (65.56%), Query Frame = 1


Query: 1    MEKLKGLKAILKSWNKETFGKIFSQKQVLIDKINYLDSLEESSCLNEENVKERENCRGAL 60
            M KL+ LKA LK WNK  FG +  +K+ ++  I   DS+E+   L+ E + +R   +G L
Sbjct: 1002 MRKLQFLKAKLKEWNKNAFGDLIERKKCILLDIANFDSMEQEGGLSPELLIQRAVRKGEL 1061

Query: 61   LDLIVKEQKLWIQKSKLHWLREGEENSSFFHIWVSARKSKSILSSLVSIEGKTLVTEKEI 120
             +LI++E+  W QK+++ W++EG+ NS FFH   + R+++  +  L +  G  L     I
Sbjct: 1062 EELILREEIHWRQKARVKWVKEGDCNSKFFHKVANGRRNRKFIKVLENERGLVLDNSDSI 1121

Query: 121  VDEILSFFSNLYGTRISSPFICDILNWRGLSLQDSSLLEVPFTEKEIREVVFEMGCLKSP 180
             +EIL +F  LY +     +  + L+W  +S + +S LE PFTE+EI + +F+M   K+P
Sbjct: 1122 KEEILRYFEKLYASPSGESWRVEGLDWSPISSESASRLESPFTEEEISKAIFQMDRDKAP 1181

Query: 181  GPDGLTGEFYKKSWNILKSDLVRVFQDFFKNGIINRRCNETYIYLIPKKKEAARVSDFRP 240
            GPDG T   ++  W+++K DLVRVF +F ++GIIN+  N ++I L+PKK  A ++SD+RP
Sbjct: 1182 GPDGFTIAVFQDCWDVIKEDLVRVFDEFHRSGIINQSTNASFIVLLPKKSMAKKLSDYRP 1241

Query: 241  ISLITSLYKVISKVLPTRLKKVLPSIINDSQMAFVEGRQILDAILTASEAVDEWSLRGRK 300
            ISLITSLYK+I+KVL  RL+ VL   I+ +Q AFV+GRQILDA+L A+E VDE      +
Sbjct: 1242 ISLITSLYKIIAKVLAGRLRGVLHETIHSTQGAFVQGRQILDAVLIANEIVDEKKRSXEE 1301

Query: 301  GVLLKLDLEKAYDKVDWSFLDMAMKLKGFGKRCRKWIWGCLSTTNFSIIVNGRPRGKIIA 360
            GV+ K+D EKAYD V W FLD  M+ KGF  R RKWI GCLS+ +F+I+VNG  +G + A
Sbjct: 1302 GVVFKIDFEKAYDHVSWDFLDHVMEKKGFNPRWRKWIRGCLSSVSFAILVNGNAKGWVKA 1361

Query: 361  KRGIRQGDPLAPFLFTIVGDAPSCLIHYCNEKRSLKGFHFENLSEDLTHLQYADDTLLSS 420
             RG+RQGDPL+PFLFTIV D  S ++    E+   +GF        ++HLQ+ADDT+  S
Sbjct: 1362 SRGLRQGDPLSPFLFTIVADVMSRMLLRAEERNVFEGFRVGRNRTRVSHLQFADDTIFFS 1421

Query: 421  S 422
            S
Sbjct: 1422 S 1422

The following BLAST results are available for this feature:

BLAST of CSPI04G20730 vs. Swiss-Prot


Match Name	E-value	Identity	Description
YTX2_XENLA	2.2e-38	27.27	Transposon TX1 uncharacterized 149 kDa protein OS=Xenopus laevis PE=3 SV=1	[more]
LIN1_NYCCO	6.8e-32	27.01	LINE-1 reverse transcriptase homolog OS=Nycticebus coucang PE=3 SV=1	[more]
LORF2_HUMAN	1.7e-30	26.59	LINE-1 retrotransposable element ORF2 protein OS=Homo sapiens PE=1 SV=1	[more]
LORF2_MOUSE	2.4e-29	28.13	LINE-1 retrotransposable element ORF2 protein OS=Mus musculus GN=Pol PE=1 SV=2	[more]
PO22_POPJA	2.0e-15	29.27	Retrovirus-related Pol polyprotein from type-1 retrotransposable element R2 (Fra...	[more]

BLAST of CSPI04G20730 vs. TrEMBL


Match Name	E-value	Identity	Description
A5BV95_VITVI	3.8e-106	45.24	Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_026478 PE=4 SV=1	[more]
M5XUF8_PRUPE	3.0e-103	46.02	Uncharacterized protein (Fragment) OS=Prunus persica GN=PRUPE_ppa015473mg PE=4 S...	[more]
A5CAA2_VITVI	5.1e-103	44.66	Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_030956 PE=4 SV=1	[more]
A5BH71_VITVI	1.5e-102	44.89	Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_013913 PE=4 SV=1	[more]
A5BQD9_VITVI	1.9e-102	45.37	Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_000232 PE=4 SV=1	[more]

BLAST of CSPI04G20730 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G43760.1	1.3e-25	30.15	DNAse I-like superfamily protein	[more]
ATMG01250.1	7.0e-11	42.65	ATMG01250.1 RNA-directed DNA polymerase (reverse transcriptase)	[more]
AT4G20520.1	6.6e-09	43.42	RNA binding;RNA-directed DNA polymerases	[more]

BLAST of CSPI04G20730 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|147784237\|emb\|CAN75040.1\|	5.4e-106	45.24	hypothetical protein VITISV_026478 [Vitis vinifera]	[more]
gi\|596221970\|ref\|XP_007224079.1\|	4.3e-103	46.02	hypothetical protein PRUPE_ppa015473mg, partial [Prunus persica]	[more]
gi\|147803328\|emb\|CAN68838.1\|	7.4e-103	44.66	hypothetical protein VITISV_030956 [Vitis vinifera]	[more]
gi\|147815144\|emb\|CAN67932.1\|	2.1e-102	44.89	hypothetical protein VITISV_013913 [Vitis vinifera]	[more]
gi\|147857332\|emb\|CAN79190.1\|	2.8e-102	45.37	hypothetical protein VITISV_000232 [Vitis vinifera]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR000477	RT_dom

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI04G20730.1	CSPI04G20730.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000477	Reverse transcriptase domain	PFAM	PF00078	RVT_1	coord: 226..420 score: 1.3
IPR000477	Reverse transcriptase domain	PROFILE	PS50878	RT_POL	coord: 207..421 score: 15
None	No IPR available	PANTHER	PTHR19446	REVERSE TRANSCRIPTASES	coord: 48..378 score: 4.1E-101coord: 2..31 score: 4.1E
None	No IPR available	PANTHER	PTHR19446:SF368	SUBFAMILY NOT NAMED	coord: 2..31 score: 4.1E-101coord: 48..378 score: 4.1E
None	No IPR available	unknown	SSF56672	DNA/RNA polymerases	coord: 178..420 score: 1.78

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CSPI04G20730	Cucsa.357490	Cucumber (Gy14) v1	cgycpiB529

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CSPI04G20730	Melon (DHL92) v3.6.1	cpimedB324
CSPI04G20730	Cucumber (Gy14) v2	cgybcpiB169
CSPI04G20730	Silver-seed gourd	carcpiB0315
CSPI04G20730	Silver-seed gourd	carcpiB0894
CSPI04G20730	Cucumber (Chinese Long) v3	cpicucB212
CSPI04G20730	Watermelon (97103) v2	cpiwmbB306
CSPI04G20730	Watermelon (97103) v2	cpiwmbB308
CSPI04G20730	Wax gourd	cpiwgoB401
CSPI04G20730	Cucurbita maxima (Rimu)	cmacpiB679
CSPI04G20730	Cucurbita maxima (Rimu)	cmacpiB878
CSPI04G20730	Cucurbita moschata (Rifu)	cmocpiB670
CSPI04G20730	Cucurbita moschata (Rifu)	cmocpiB858
CSPI04G20730	Cucumber (Chinese Long) v2	cpicuB181
CSPI04G20730	Melon (DHL92) v3.5.1	cpimeB333
CSPI04G20730	Watermelon (Charleston Gray)	cpiwcgB319
CSPI04G20730	Watermelon (97103) v1	cpiwmB338
CSPI04G20730	Cucurbita pepo (Zucchini)	cpecpiB066
CSPI04G20730	Cucurbita pepo (Zucchini)	cpecpiB500
CSPI04G20730	Bottle gourd (USVL1VR-Ls)	cpilsiB258