CSPI04G16940 (gene) Wild cucumber (PI 183967)

Overview
NameCSPI04G16940
Typegene
OrganismCucumis sativus (Wild cucumber (PI 183967))
DescriptionAlcohol dehydrogenase-like protein
LocationChr4 : 14368025 .. 14371145 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGCATTTTGGTGAAAGTTAGAGGCATTCATATCTGAGTTTTTAAGACTTGACATTTAAACTAAATTTTTAAGAAGGCATTTAGCCCAGAAGTCTTCCCAACCAATCTGGTGTCTTTTCCCCTTCTTTTTGTAACATTATTTCTGATTTCTCCAGTTGCTTGCAGATAGCAAGGGCCTTCGGTGCTTCTCATATTATTGCCGTTGATGTTCAGGATGATAAACTGCTAAAAGCAAAGACAATTGGTGCTACTCATACCATTAATTCTCGGAAAGAAGACGCTATTGAAAAGATAAGAGTATGTACTTTATCCCTTGATAAAAGTTTCCAAGATTCTGCCCAAGAACAAGTTATCCTATGTAGGCTAAATTATACAAAATGACCTTGAAGTTTGCACTTAGTGTCAAAAAGACTCCTAAACTTTAAGAAATACACTTAAAATTTCAATAAGAGTTCAAAAATACCCTTGTCATTAGTTTTCAACCCTTATCGTCAGTTTTGAATGGAAACGAAAGGTTTTGTTTCAAAAGATCCTTAAACTTAAAAGAAAGTTAAAATAGTACCCTTACTTTCTATATATGAACAGAAACCGTTAATATTTTGTTACATAAATGCCTATATGCTTTCAAAAGTTGCATTCGTACCTTAACAAAATAAAAAATAAAAAGGAAAAGTATTGTAAATGATAAAATAACTGAAAATATTTACAAAATATAACAAAATTTTAGATTCTATCAATGATAGAAGTTAATGTTAGATACTGATAAACTTTTATCTGTTTAGGGTTTAGAATCCAAAATTTAGCTATATTGTGTAAATATTTTTGGTTTATTTTGCTATATTTGAAAATGAGATAAATAATAAGTAAATAGAAGTAAATAAATGTAAAAATGCTCTTGGATTTTTATGCTTTAACTCGAAGTTTTGGTTTATATTTTGTTGTGACACAATTGGTGTAAGAATCAGACAAATGAGAGCAAAATATAGCATCTATATAAGAAAAAAATGCTGCCTATATAATTTGTTTCAAACTTGGTTCTTTTCCAAGTTGAGAAAAGTTCAAACAAATTGTCATTATATTGATGGTAGGACGATTTTTTTTACTGGCTATTAATGTAACTTTTAAAAGTCCTAGGGATCTTTGAAATGAGTATTAACGGTATGTATTCGTATACTAACAGTAAGGGTATTTTTTAATCTTTTTGAAAGTTCAAGGGTATTTTTGAAACCAAACACTAATGGTTTCCATTCAAAACTAACAAGGGTACTTTTGTATTGTTTTTGTAAGTGCAAGGGTATTTTTGAAACTTTTGGAAGTTCAAGAATATATTTGACACAAAGTATAAAGTTTAGGGTATTTGGTATAATTTAGTCTCATATGTATCATCATCATGGGTGTTGAATCTTGTATATAATATTGAATAACTAGAACATATTGAATAATTAGCAAAATTTTACAAATGTGATTGGAAGATTAAAACGAACAGACCCAACAAAAGAAAGAAACAGTGAATAAAAAATACCGGAAAGGAAAATATAGATAAAGGAAAAATAAACCATAATTTACTTTAACGCCCTCCCTCAAACTCAAGTTGAGTTAGAGTTGGTCTAACTCAAGGTGGTTGAGTGACGGCCAACTTGAGTTTGTGAAGTGTACGAACTGAATCGAATAGATGAAATTTAAGGTGGCAGATGACAAAAATTGAAACAAATAATGACATATTTGGGCTGCAAAAAAAACCACGAAGAACTTTTGGGTGGGAGACCGAGGTTGAAACTGAAAAACAAAGGACTAGAACGCAAAATGAACTAAGCATGAACCAAATTGAACCAGGCTGGTCTAAAAGCTAAAAGTGAAGACAAGTAGTAGCACCACATGTCTTCACTTTTAGCAATTTACGAGTATTAATGGTACAACATTTGGAAGAAAACAAATAATAGAGACTTGAAAATGTCGAACAGACCGGGTATGGGTCTATCCCCCACCCACTGGTTTTCTTGAAATTAATATCCTTCCCTTTCCCTACCATTCGACGGACCAAAAGAGAGAGAAGGTAACTCTTTAAAATTATCATTATATAAGCTTTGACCTCACAAATTTTATACTTGGATTATTTCGGCTTATATGATATGTGAAAGTTAAAGATTCTTTAAGCTTTTGTTTATGAAACTGGAGGGTCTATCTTTTTTTTTTTTTGGAGTTCTATTTTGCTGCAATGTAAGTAACTGAGGTGGTGACAGGAAATAACAGGAGGAATGGGAGTGGACGTTGCTGTGGAGGCATTGGGAAGGCCACAGACGTTCATGCAGTGTACTCAAAGTGTGAAAGATGGAGGTAAAGCTGTAATGATTGGACTTGCTCAAGCTGGTTCTGTAGGCGAGATTGATATTAACCGCCTTGTTCGTAGAAAGGTCTGCCTTTTCTTCTTTTGTTAATGTTCTCTCCCTGAGGTCGCATTATCAGCACGTTTCTTCAGTGTCCATTTTTTATTTCTCCAATTTAATGAGTATGTGGTGGTACTAACTTTGGTATTATGCCAACTGGCAAATACTTCTAGTAACACTGTTAGGCGTCTATTGTTAAATATGTATGCTAGGTGGAGAAAAATGGGGAACTGTGAAGTATTTAGTTATTATGTATGCTAGGAGGAGCAAAATGGGGAATCGAGTGAAGTAGTTATGTTAGAATGGTGACTCATATAGGTGTAGAAAAGGGGTAGTGAAAGGGTATCTGTTATTTCGTGAAGAGTAGCAATTTATATTCTTGCTACAGATTGTAATTGTAGCAGCTCTAGCTTTCGATTATAACAACATATGTTTAATAAGGCTCCTTGAACTGGAAGTTTTTGCCTCTTATCTCTTTATGTACACTATGTGCTGAGTTTGTTGACAATATTTTACTTCAAATAAAATGCTGAGTTGGTGCAGATAAAAGTGATTGGGTCTTATGGAGGAAGAGCAAGGCAGGATCTGCCCAAGTTGGTTAAATTGGCAGAATCAGGAATTTTCAATCTTAGTGATGCAGTTTCTCGAAAGTACAAGTTTGAGGATTCAAACCAAGCATTTCAAGACCTCAATGAAGGAAAAATCATCGGTCGAGCTGTTATAGAGATTGACTAA

mRNA sequence

ATGGGCATTTTGGTGAAAATAGCAAGGGCCTTCGGTGCTTCTCATATTATTGCCGTTGATGTTCAGGATGATAAACTGCTAAAAGCAAAGACAATTGGTGCTACTCATACCATTAATTCTCGGAAAGAAGACGCTATTGAAAAGATAAGAGAAATAACAGGAGGAATGGGAGTGGACGTTGCTGTGGAGGCATTGGGAAGGCCACAGACGTTCATGCAGTGTACTCAAAGTGTGAAAGATGGAGGTAAAGCTGTAATGATTGGACTTGCTCAAGCTGGTTCTGTAGGCGAGATTGATATTAACCGCCTTGTTCGTAGAAAGATAAAAGTGATTGGGTCTTATGGAGGAAGAGCAAGGCAGGATCTGCCCAAGTTGGTTAAATTGGCAGAATCAGGAATTTTCAATCTTAGTGATGCAGTTTCTCGAAAGTACAAGTTTGAGGATTCAAACCAAGCATTTCAAGACCTCAATGAAGGAAAAATCATCGGTCGAGCTGTTATAGAGATTGACTAA

Coding sequence (CDS)

ATGGGCATTTTGGTGAAAATAGCAAGGGCCTTCGGTGCTTCTCATATTATTGCCGTTGATGTTCAGGATGATAAACTGCTAAAAGCAAAGACAATTGGTGCTACTCATACCATTAATTCTCGGAAAGAAGACGCTATTGAAAAGATAAGAGAAATAACAGGAGGAATGGGAGTGGACGTTGCTGTGGAGGCATTGGGAAGGCCACAGACGTTCATGCAGTGTACTCAAAGTGTGAAAGATGGAGGTAAAGCTGTAATGATTGGACTTGCTCAAGCTGGTTCTGTAGGCGAGATTGATATTAACCGCCTTGTTCGTAGAAAGATAAAAGTGATTGGGTCTTATGGAGGAAGAGCAAGGCAGGATCTGCCCAAGTTGGTTAAATTGGCAGAATCAGGAATTTTCAATCTTAGTGATGCAGTTTCTCGAAAGTACAAGTTTGAGGATTCAAACCAAGCATTTCAAGACCTCAATGAAGGAAAAATCATCGGTCGAGCTGTTATAGAGATTGACTAA
Homology
[Close]
BLAST of CSPI04G16940 vs. Swiss-Prot
Match: SUCD_METS5 (Succinate-semialdehyde dehydrogenase (acetylating) OS=Metallosphaera sedula (strain ATCC 51363 / DSM 5348) GN=Msed_1424 PE=1 SV=1)

HSP 1 Score: 120.2 bits (300), Expect = 2.3e-26
Identity = 65/163 (39.88%), Postives = 102/163 (62.58%), Query Frame = 1 

Query: 5   VKIARAFGASHIIAVDVQDDKLLKAKTIGATHTINSRKEDAIEKIREITGGMGVDVAVEA 64
           +++ RA GA  IIAV  +  KL +A  +GAT  +NS++ D ++ I+EITGG G  V +EA
Sbjct: 198 IQLLRASGAGRIIAVGTKKWKLDRAMELGATDVVNSKEIDPVKAIKEITGG-GPQVVIEA 257

Query: 65  LGRPQTFMQCTQSVKDGGKAVMIGLAQAGSVGEIDINRLVRRKIKVIGSYGGRARQDLPK 124
            G   T      SV+ GGK V++GL  A ++  I +  +VR  I+V+G+YGGR R D+PK
Sbjct: 258 GGNEDTIHMALDSVRIGGKVVLVGLPPATAMIPIRVASIVRGGIEVVGNYGGRPRVDMPK 317

Query: 125 LVKLAESGIFNLSDAVSRKYKFEDSNQAFQDLNEGKIIGRAVI 168
           L++L   G ++ S  V+ +++ E+ N+A + L EG+ I   +I
Sbjct: 318 LLELVRQGRYDPSRLVTGRFRLEEINEAVKMLEEGEAIRSLII 359

[Close]
BLAST of CSPI04G16940 vs. Swiss-Prot
Match: DHSO_BACSU (Sorbitol dehydrogenase OS=Bacillus subtilis (strain 168) GN=gutB PE=1 SV=3)

HSP 1 Score: 90.9 bits (224), Expect = 1.5e-17
Identity = 52/150 (34.67%), Postives = 80/150 (53.33%), Query Frame = 1 

Query: 5   VKIARAFGASHIIAVDVQDDKLLKAKTIGATHTINSRKEDAIEKIREITGGMGVDVAVEA 64
           V  A+AFGA  II  D++  +L  AK +GATH IN R++DA+E+I+ IT   GVDVA E 
Sbjct: 189 VAAAKAFGAGTIIVTDLEPLRLEAAKKMGATHIINIREQDALEEIKTITNDRGVDVAWET 248

Query: 65  LGRPQTFMQCTQSVKDGGKAVMIGLAQAGSVGEIDINRLVRRKIKVIGSYGGRARQDLPK 124
            G P        SV+ GGK  ++GL     +  +++  +   +I + G +  R     PK
Sbjct: 249 AGNPAALQSALASVRRGGKLAIVGLPSQNEI-PLNVPFIADNEIDIYGIF--RYANTYPK 308

Query: 125 LVKLAESGIFNLSDAVSRKYKFEDSNQAFQ 155
            ++   SGI +    V+ +Y  E +  A +
Sbjct: 309 GIEFLASGIVDTKHLVTDQYSLEQTQDAME 335

[Close]
BLAST of CSPI04G16940 vs. Swiss-Prot
Match: TDH_CHRVO (L-threonine 3-dehydrogenase OS=Chromobacterium violaceum (strain ATCC 12472 / DSM 30191 / JCM 1249 / NBRC 12614 / NCIMB 9131 / NCTC 9757) GN=tdh PE=3 SV=1)

HSP 1 Score: 90.1 bits (222), Expect = 2.5e-17
Identity = 51/169 (30.18%), Postives = 96/169 (56.80%), Query Frame = 1 

Query: 1   MGIL-VKIARAFGASHIIAVDVQDDKLLKAKTIGATHTINSRKEDAIEKIREITGGMGVD 60
           +GI+ V IA+  GA H++  DV D +L  AK +GAT  +N  +ED    ++E+    G D
Sbjct: 175 IGIMAVAIAKHVGARHVVITDVNDYRLELAKKMGATRAVNVAREDLKAVMQELHMSEGFD 234

Query: 61  VAVEALGRPQTFMQCTQSVKDGGKAVMIGLAQAGSVGEIDINRLVRRKIKVIGSYGGRAR 120
           V +E  G PQ F Q  +++  GGK  ++G+  + +   ID N+++ + +++ G YG    
Sbjct: 235 VGLEMSGNPQAFRQMLETMNHGGKVALLGIPPSNTA--IDWNQVIFKGLEIKGIYGREMF 294

Query: 121 QDLPKLVKLAESGIFNLSDAVSRKYKFEDSNQAFQDLNEGKIIGRAVIE 169
           +   K+V L +SG+ ++S  ++  +K ++  Q F  +  G+  G+ +++
Sbjct: 295 ETWYKMVALIQSGL-DISPIITHHFKVDEFEQGFAAMLSGQ-SGKVILD 339

[Close]
BLAST of CSPI04G16940 vs. Swiss-Prot
Match: FADH_AMYME (S-(hydroxymethyl)mycothiol dehydrogenase OS=Amycolatopsis methanolica PE=1 SV=2)

HSP 1 Score: 89.0 bits (219), Expect = 5.6e-17
Identity = 51/162 (31.48%), Postives = 85/162 (52.47%), Query Frame = 1 

Query: 8   ARAFGASHIIAVDVQDDKLLKAKTIGATHTINSRKEDAIEKIREITGGMGVDVAVEALGR 67
           AR  GA+ IIAVD    KL  A  +GATHT+N+ + D +E ++ +TGG G DV ++A+GR
Sbjct: 198 ARLAGANKIIAVDRDAKKLEWATELGATHTVNATETDVVEAVQALTGGFGADVVIDAVGR 257

Query: 68  PQTFMQCTQSVKDGGKAVMIGLAQAGSVGEIDINRLVRRKIKVIGSYGGRA--RQDLPKL 127
           P+T+ Q   +    G  V++G+       E+ +     R   +  S+ G     +D P L
Sbjct: 258 PETWKQAFYARDLAGTVVLVGVPTPDMRLEMPLLDFFSRGGALKSSWYGDCLPERDFPVL 317

Query: 128 VKLAESGIFNLSDAVSRKYKFEDSNQAFQDLNEGKIIGRAVI 168
           + L   G   L   V+ +   +D  +AF  ++ G+++   V+
Sbjct: 318 IDLHLQGRLPLDKFVTERISLDDVEKAFHTMHAGEVLRSVVV 359

[Close]
BLAST of CSPI04G16940 vs. Swiss-Prot
Match: TDH_BACVZ (L-threonine 3-dehydrogenase OS=Bacillus velezensis (strain DSM 23117 / BGSC 10A6 / FZB42) GN=tdh PE=3 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 1.4e-15
Identity = 50/156 (32.05%), Postives = 85/156 (54.49%), Query Frame = 1 

Query: 5   VKIARAFGASHIIAVDVQDDKLLKAKTIGATHTINSRKEDAIEKIREITGGMGVDVAVEA 64
           V +A+A GAS I A+D  D +L  AK +GAT TI++ KED +E I  +TGG G+D+  E 
Sbjct: 185 VAVAKAAGASRIAAIDKNDYRLALAKKLGATITISADKEDPLEVIDTLTGGEGIDLVCEM 244

Query: 65  LGRPQTFMQCTQSVKDGGKAVMIGLAQAGSVGEIDINRLVRRKIKVIGSYGGRARQDLPK 124
            G P    Q  +   +GG+  M+ L +     ++  N +V + + V G  G +  +   +
Sbjct: 245 SGHPAAIAQGLKMAANGGRFHMLSLPERPVTVDL-TNDVVFKGLTVQGITGRKMFETWRQ 304

Query: 125 LVKLAESGIFNLSDAVSRKYKFEDSNQAFQDLNEGK 161
           +  L ESG+ +L+  ++ +   ED  + F  + +G+
Sbjct: 305 VSHLLESGLVDLTPVITHQLPLEDFEKGFDLMRKGQ 339

[Close]
BLAST of CSPI04G16940 vs. TrEMBL
Match: A0A0A0KXY5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G337910 PE=4 SV=1)

HSP 1 Score: 328.2 bits (840), Expect = 6.1e-87
Identity = 170/170 (100.00%), Postives = 170/170 (100.00%), Query Frame = 1 

Query: 1   MGILVKIARAFGASHIIAVDVQDDKLLKAKTIGATHTINSRKEDAIEKIREITGGMGVDV 60
           MGILVKIARAFGASHIIAVDVQDDKLLKAKTIGATHTINSRKEDAIEKIREITGGMGVDV
Sbjct: 1   MGILVKIARAFGASHIIAVDVQDDKLLKAKTIGATHTINSRKEDAIEKIREITGGMGVDV 60

Query: 61  AVEALGRPQTFMQCTQSVKDGGKAVMIGLAQAGSVGEIDINRLVRRKIKVIGSYGGRARQ 120
           AVEALGRPQTFMQCTQSVKDGGKAVMIGLAQAGSVGEIDINRLVRRKIKVIGSYGGRARQ
Sbjct: 61  AVEALGRPQTFMQCTQSVKDGGKAVMIGLAQAGSVGEIDINRLVRRKIKVIGSYGGRARQ 120

Query: 121 DLPKLVKLAESGIFNLSDAVSRKYKFEDSNQAFQDLNEGKIIGRAVIEID 171
           DLPKLVKLAESGIFNLSDAVSRKYKFEDSNQAFQDLNEGKIIGRAVIEID
Sbjct: 121 DLPKLVKLAESGIFNLSDAVSRKYKFEDSNQAFQDLNEGKIIGRAVIEID 170

[Close]
BLAST of CSPI04G16940 vs. TrEMBL
Match: A0A078D5J5_BRANA (BnaC03g50730D protein OS=Brassica napus GN=BnaC03g50730D PE=4 SV=1)

HSP 1 Score: 283.9 bits (725), Expect = 1.3e-73
Identity = 142/165 (86.06%), Postives = 157/165 (95.15%), Query Frame = 1 

Query: 5   VKIARAFGASHIIAVDVQDDKLLKAKTIGATHTINSRKEDAIEKIREITGGMGVDVAVEA 64
           ++IARAFGAS IIAVDVQDDKL KAKT+GATHT+N+ KEDA+E+IREITGGMGVDVAVEA
Sbjct: 270 LQIARAFGASDIIAVDVQDDKLEKAKTLGATHTVNAAKEDAVERIREITGGMGVDVAVEA 329

Query: 65  LGRPQTFMQCTQSVKDGGKAVMIGLAQAGSVGEIDINRLVRRKIKVIGSYGGRARQDLPK 124
           LGRPQTFMQCT SVKDGGKAVMIGL+QAGSVGEIDINRLVRRKIKVIGSYGGRARQDLPK
Sbjct: 330 LGRPQTFMQCTLSVKDGGKAVMIGLSQAGSVGEIDINRLVRRKIKVIGSYGGRARQDLPK 389

Query: 125 LVKLAESGIFNLSDAVSRKYKFEDSNQAFQDLNEGKIIGRAVIEI 170
           +VKLAESGIFNL++AVS KYKFED+ +AFQDLNEGKI+ R V+EI
Sbjct: 390 VVKLAESGIFNLTNAVSSKYKFEDAGKAFQDLNEGKIVSRGVVEI 434

[Close]
BLAST of CSPI04G16940 vs. TrEMBL
Match: A0A0D3BFD4_BRAOL (Uncharacterized protein (Fragment) OS=Brassica oleracea var. oleracea PE=4 SV=1)

HSP 1 Score: 283.5 bits (724), Expect = 1.7e-73
Identity = 142/165 (86.06%), Postives = 156/165 (94.55%), Query Frame = 1 

Query: 5   VKIARAFGASHIIAVDVQDDKLLKAKTIGATHTINSRKEDAIEKIREITGGMGVDVAVEA 64
           ++IARAFGAS IIAVDVQDDKL KAKT+GATHT+N+ KEDA+E+IREITGGMGVDVAVEA
Sbjct: 307 LQIARAFGASDIIAVDVQDDKLEKAKTLGATHTVNAAKEDAVERIREITGGMGVDVAVEA 366

Query: 65  LGRPQTFMQCTQSVKDGGKAVMIGLAQAGSVGEIDINRLVRRKIKVIGSYGGRARQDLPK 124
           LGRPQTFMQCT SVKDGGKAVMIGL+QAGSVGEIDINRLVRRKIKVIGSYGGRARQDLPK
Sbjct: 367 LGRPQTFMQCTLSVKDGGKAVMIGLSQAGSVGEIDINRLVRRKIKVIGSYGGRARQDLPK 426

Query: 125 LVKLAESGIFNLSDAVSRKYKFEDSNQAFQDLNEGKIIGRAVIEI 170
           +VKLAESGIFNL +AVS KYKFED+ +AFQDLNEGKI+ R V+EI
Sbjct: 427 VVKLAESGIFNLKNAVSSKYKFEDAGKAFQDLNEGKIVSRGVVEI 471

[Close]
BLAST of CSPI04G16940 vs. TrEMBL
Match: A0A078HW01_BRANA (BnaA06g22770D protein OS=Brassica napus GN=BnaA06g22770D PE=4 SV=1)

HSP 1 Score: 282.3 bits (721), Expect = 3.8e-73
Identity = 141/165 (85.45%), Postives = 157/165 (95.15%), Query Frame = 1 

Query: 5   VKIARAFGASHIIAVDVQDDKLLKAKTIGATHTINSRKEDAIEKIREITGGMGVDVAVEA 64
           ++IARAFGAS IIAVDVQDDKL KAKT+GATHT+N+ KEDA+E+IREITGGMGVDVAVEA
Sbjct: 270 LQIARAFGASDIIAVDVQDDKLEKAKTLGATHTVNAAKEDAVERIREITGGMGVDVAVEA 329

Query: 65  LGRPQTFMQCTQSVKDGGKAVMIGLAQAGSVGEIDINRLVRRKIKVIGSYGGRARQDLPK 124
           LGRPQTFMQCT SVKDGGKAVMIGL++AGSVGEIDINRLVRRKIKVIGSYGGRARQDLPK
Sbjct: 330 LGRPQTFMQCTLSVKDGGKAVMIGLSKAGSVGEIDINRLVRRKIKVIGSYGGRARQDLPK 389

Query: 125 LVKLAESGIFNLSDAVSRKYKFEDSNQAFQDLNEGKIIGRAVIEI 170
           +VKLAESGIFNL++AVS KYKFED+ +AFQDLNEGKI+ R V+EI
Sbjct: 390 VVKLAESGIFNLTNAVSSKYKFEDAGKAFQDLNEGKIVSRGVVEI 434

[Close]
BLAST of CSPI04G16940 vs. TrEMBL
Match: M4FC33_BRARP (Uncharacterized protein OS=Brassica rapa subsp. pekinensis PE=4 SV=1)

HSP 1 Score: 282.3 bits (721), Expect = 3.8e-73
Identity = 141/165 (85.45%), Postives = 157/165 (95.15%), Query Frame = 1 

Query: 5   VKIARAFGASHIIAVDVQDDKLLKAKTIGATHTINSRKEDAIEKIREITGGMGVDVAVEA 64
           ++IARAFGAS IIAVDVQDDKL KAKT+GATHT+N+ KEDA+E+IREITGGMGVDVAVEA
Sbjct: 270 LQIARAFGASDIIAVDVQDDKLEKAKTLGATHTVNAAKEDAVERIREITGGMGVDVAVEA 329

Query: 65  LGRPQTFMQCTQSVKDGGKAVMIGLAQAGSVGEIDINRLVRRKIKVIGSYGGRARQDLPK 124
           LGRPQTFMQCT SVKDGGKAVMIGL++AGSVGEIDINRLVRRKIKVIGSYGGRARQDLPK
Sbjct: 330 LGRPQTFMQCTLSVKDGGKAVMIGLSKAGSVGEIDINRLVRRKIKVIGSYGGRARQDLPK 389

Query: 125 LVKLAESGIFNLSDAVSRKYKFEDSNQAFQDLNEGKIIGRAVIEI 170
           +VKLAESGIFNL++AVS KYKFED+ +AFQDLNEGKI+ R V+EI
Sbjct: 390 VVKLAESGIFNLTNAVSSKYKFEDAGKAFQDLNEGKIVSRGVVEI 434

[Close]
BLAST of CSPI04G16940 vs. TAIR10
Match: AT5G63620.1 (AT5G63620.1 GroES-like zinc-binding alcohol dehydrogenase family protein)

HSP 1 Score: 280.8 bits (717), Expect = 5.6e-76
Identity = 140/165 (84.85%), Postives = 156/165 (94.55%), Query Frame = 1 

Query: 5   VKIARAFGASHIIAVDVQDDKLLKAKTIGATHTINSRKEDAIEKIREITGGMGVDVAVEA 64
           ++IARAFGAS IIAVDVQDDKL KAKT+GATH +N+ KEDA+E+IREITGGMGVDVAVEA
Sbjct: 262 LQIARAFGASDIIAVDVQDDKLQKAKTLGATHIVNAAKEDAVERIREITGGMGVDVAVEA 321

Query: 65  LGRPQTFMQCTQSVKDGGKAVMIGLAQAGSVGEIDINRLVRRKIKVIGSYGGRARQDLPK 124
           LG+PQTFMQCT SVKDGGKAVMIGL+QAGSVGEIDINRLVRRKIKVIGSYGGRARQDLPK
Sbjct: 322 LGKPQTFMQCTLSVKDGGKAVMIGLSQAGSVGEIDINRLVRRKIKVIGSYGGRARQDLPK 381

Query: 125 LVKLAESGIFNLSDAVSRKYKFEDSNQAFQDLNEGKIIGRAVIEI 170
           +VKLAESGIFNL++AVS KYKFED+ +AFQDLNEGKI+ R V+EI
Sbjct: 382 VVKLAESGIFNLTNAVSSKYKFEDAGKAFQDLNEGKIVSRGVVEI 426

[Close]
BLAST of CSPI04G16940 vs. TAIR10
Match: AT1G32780.1 (AT1G32780.1 GroES-like zinc-binding dehydrogenase family protein)

HSP 1 Score: 68.9 bits (167), Expect = 3.4e-12
Identity = 54/167 (32.34%), Postives = 80/167 (47.90%), Query Frame = 1 

Query: 8   ARAFGASHIIAVDVQDDKLLKAKTIGATHTINSRK--EDAIEKIREITGGMGVDVAVEAL 67
           ARA GAS II VD    K  K K +G T  IN +   +   + IREITGG GVD + E  
Sbjct: 225 ARARGASRIIGVDANASKFEKGKLMGVTDFINPKDLTKPVHQMIREITGG-GVDYSFECT 284

Query: 68  GRPQTFMQCTQSVKDG-GKAVMIGLAQAGSVGEIDINRLVRRKIKVIGS-YGG-RARQDL 127
           G      +   S   G G  V++G+        +    L   + ++ GS +GG + +  L
Sbjct: 285 GNVDVLREAFLSTHVGWGSTVLVGIYPTPRTLPLHPMELFDGR-RITGSVFGGFKPKSQL 344

Query: 128 PKLVKLAESGIFNLSDAVSRKYKFEDSNQAFQDLNEGKIIGRAVIEI 170
           P   +    G+  L   ++ +  FE  N AFQ L +GK + R +++I
Sbjct: 345 PNFAQQCMKGVVKLEPFITNELPFEKINDAFQLLRDGKSL-RCILQI 388

[Close]
BLAST of CSPI04G16940 vs. TAIR10
Match: AT5G42250.1 (AT5G42250.1 Zinc-binding alcohol dehydrogenase family protein)

HSP 1 Score: 68.6 bits (166), Expect = 4.4e-12
Identity = 54/161 (33.54%), Postives = 78/161 (48.45%), Query Frame = 1 

Query: 8   ARAFGASHIIAVDVQDDKLLKAKTIGATHTINS---RKEDAIEKIREITGGMGVDVAVEA 67
           AR  GAS II VD+   K    +  G T  +NS    K    E I E+T G G D   E 
Sbjct: 224 ARLCGASRIIGVDINPTKFQVGQKFGVTEFVNSMTCEKNRVSEVINEMTDG-GADYCFEC 283

Query: 68  LGRPQTFMQCTQSVKDG-GKAVMIGLAQAGSVGEIDINRLVRRKIKVIGS-YGG-RARQD 127
           +G      +     + G GK + +G+ + GS   +D   ++     ++GS +GG +A+  
Sbjct: 284 VGSSSLVQEAYACCRQGWGKTITLGVDKPGSQICLDSFDVLHHGKILMGSLFGGLKAKTH 343

Query: 128 LPKLVKLAESGIFNLSDAVSRKYKFEDSNQAFQDLNEGKII 163
           +P L+K   S    L   V+ + KFE+ N AFQ L EGK I
Sbjct: 344 IPILLKRYLSNELELDKFVTHEMKFEEINDAFQLLLEGKCI 383

[Close]
BLAST of CSPI04G16940 vs. TAIR10
Match: AT1G64710.1 (AT1G64710.1 GroES-like zinc-binding dehydrogenase family protein)

HSP 1 Score: 68.2 bits (165), Expect = 5.7e-12
Identity = 48/159 (30.19%), Postives = 77/159 (48.43%), Query Frame = 1 

Query: 8   ARAFGASHIIAVDVQDDKLLKAKTIGATHTINSRKEDAI--EKIREITGGMGVDVAVEAL 67
           ARA GAS II +D+  DK    +  G +  IN ++ D    E++ EIT G GV+ + E  
Sbjct: 233 ARARGASKIIGIDINPDKFQLGREAGISEFINPKESDKAVHERVMEITEG-GVEYSFECA 292

Query: 68  GRPQTFMQCTQSVKDG-GKAVMIGLAQAGSVGEIDINRLVRRKIKVIGSYGG-RARQDLP 127
           G  +   +   S   G G  VM+G+  +  +  I    L + +      +GG + +  LP
Sbjct: 293 GSIEALREAFLSTNSGVGVTVMLGVHASPQLLPIHPMELFQGRSITASVFGGFKPKTQLP 352

Query: 128 KLVKLAESGIFNLSDAVSRKYKFEDSNQAFQDLNEGKII 163
             +     G+ NL   +S +  F D N+A Q L++GK +
Sbjct: 353 FFITQCLQGLLNLDLFISHQLPFHDINEAMQLLHQGKAL 390

[Close]
BLAST of CSPI04G16940 vs. TAIR10
Match: AT4G22110.1 (AT4G22110.1 GroES-like zinc-binding dehydrogenase family protein)

HSP 1 Score: 67.8 bits (164), Expect = 7.5e-12
Identity = 57/162 (35.19%), Postives = 82/162 (50.62%), Query Frame = 1 

Query: 8   ARAFGASHIIAVDVQDDKLLKAKTIGATHTINSR---KEDAIEKIREITGGMGVDVAVEA 67
           AR  GA  II VD+  +K    K  G T  INS    +    E I+E+TGG GVD + E 
Sbjct: 223 ARLRGAGKIIGVDLNPEKFELGKKFGFTDFINSTLCGENKISEVIKEMTGG-GVDYSFEC 282

Query: 68  LGRPQTFMQCTQSVKDG-GKAVMIGLAQ---AGSVGEIDINRLVRRKIKVIGS-YGG-RA 127
           +G P    +   S + G GK V++G+ +     S+G  D+     R   V GS +GG + 
Sbjct: 283 VGLPSLLTEAFSSTRTGSGKTVVLGIDKHLTPVSLGSFDL----LRGRHVCGSLFGGLKP 342

Query: 128 RQDLPKLVKLAESGIFNLSDAVSRKYKFEDSNQAFQDLNEGK 161
           + D+P LV        NL   ++ + KFE+ N+AF  L +GK
Sbjct: 343 KLDIPILVDHYLKKELNLDSFITHELKFEEINKAFDLLVQGK 379

[Close]
BLAST of CSPI04G16940 vs. NCBI nr
Match: gi|700199327|gb|KGN54485.1| (hypothetical protein Csa_4G337910 [Cucumis sativus])

HSP 1 Score: 328.2 bits (840), Expect = 8.7e-87
Identity = 170/170 (100.00%), Postives = 170/170 (100.00%), Query Frame = 1 

Query: 1   MGILVKIARAFGASHIIAVDVQDDKLLKAKTIGATHTINSRKEDAIEKIREITGGMGVDV 60
           MGILVKIARAFGASHIIAVDVQDDKLLKAKTIGATHTINSRKEDAIEKIREITGGMGVDV
Sbjct: 1   MGILVKIARAFGASHIIAVDVQDDKLLKAKTIGATHTINSRKEDAIEKIREITGGMGVDV 60

Query: 61  AVEALGRPQTFMQCTQSVKDGGKAVMIGLAQAGSVGEIDINRLVRRKIKVIGSYGGRARQ 120
           AVEALGRPQTFMQCTQSVKDGGKAVMIGLAQAGSVGEIDINRLVRRKIKVIGSYGGRARQ
Sbjct: 61  AVEALGRPQTFMQCTQSVKDGGKAVMIGLAQAGSVGEIDINRLVRRKIKVIGSYGGRARQ 120

Query: 121 DLPKLVKLAESGIFNLSDAVSRKYKFEDSNQAFQDLNEGKIIGRAVIEID 171
           DLPKLVKLAESGIFNLSDAVSRKYKFEDSNQAFQDLNEGKIIGRAVIEID
Sbjct: 121 DLPKLVKLAESGIFNLSDAVSRKYKFEDSNQAFQDLNEGKIIGRAVIEID 170

[Close]
BLAST of CSPI04G16940 vs. NCBI nr
Match: gi|449449817|ref|XP_004142661.1| (PREDICTED: alcohol dehydrogenase 1B [Cucumis sativus])

HSP 1 Score: 318.5 bits (815), Expect = 6.9e-84
Identity = 164/166 (98.80%), Postives = 166/166 (100.00%), Query Frame = 1 

Query: 5   VKIARAFGASHIIAVDVQDDKLLKAKTIGATHTINSRKEDAIEKIREITGGMGVDVAVEA 64
           ++IARAFGASHIIAVDVQDDKLLKAKTIGATHTINSRKEDAIEKIREITGGMGVDVAVEA
Sbjct: 258 LQIARAFGASHIIAVDVQDDKLLKAKTIGATHTINSRKEDAIEKIREITGGMGVDVAVEA 317

Query: 65  LGRPQTFMQCTQSVKDGGKAVMIGLAQAGSVGEIDINRLVRRKIKVIGSYGGRARQDLPK 124
           LGRPQTFMQCTQSVKDGGKAVMIGLAQAGSVGEIDINRLVRRKIKVIGSYGGRARQDLPK
Sbjct: 318 LGRPQTFMQCTQSVKDGGKAVMIGLAQAGSVGEIDINRLVRRKIKVIGSYGGRARQDLPK 377

Query: 125 LVKLAESGIFNLSDAVSRKYKFEDSNQAFQDLNEGKIIGRAVIEID 171
           LVKLAESGIFNLSDAVSRKYKFEDSNQAFQDLNEGKIIGRAVIEID
Sbjct: 378 LVKLAESGIFNLSDAVSRKYKFEDSNQAFQDLNEGKIIGRAVIEID 423

[Close]
BLAST of CSPI04G16940 vs. NCBI nr
Match: gi|659069355|ref|XP_008449387.1| (PREDICTED: alcohol dehydrogenase 1B [Cucumis melo])

HSP 1 Score: 311.6 bits (797), Expect = 8.4e-82
Identity = 160/165 (96.97%), Postives = 164/165 (99.39%), Query Frame = 1 

Query: 5   VKIARAFGASHIIAVDVQDDKLLKAKTIGATHTINSRKEDAIEKIREITGGMGVDVAVEA 64
           ++IARAFGAS+IIAVDVQDDKLLKAKTIGATHTINS KEDAIEKIREITGGMGVD+AVEA
Sbjct: 258 LQIARAFGASYIIAVDVQDDKLLKAKTIGATHTINSHKEDAIEKIREITGGMGVDIAVEA 317

Query: 65  LGRPQTFMQCTQSVKDGGKAVMIGLAQAGSVGEIDINRLVRRKIKVIGSYGGRARQDLPK 124
           LGRPQTFMQCTQSVKDGGKAVMIGLAQAGSVGEIDINRLVRRKIKVIGSYGGRARQDLPK
Sbjct: 318 LGRPQTFMQCTQSVKDGGKAVMIGLAQAGSVGEIDINRLVRRKIKVIGSYGGRARQDLPK 377

Query: 125 LVKLAESGIFNLSDAVSRKYKFEDSNQAFQDLNEGKIIGRAVIEI 170
           LVKLAESGIFNLSDAVSRKYKFEDSNQAFQDLNEGKIIGRAVIEI
Sbjct: 378 LVKLAESGIFNLSDAVSRKYKFEDSNQAFQDLNEGKIIGRAVIEI 422

[Close]
BLAST of CSPI04G16940 vs. NCBI nr
Match: gi|674949008|emb|CDX84427.1| (BnaC03g50730D [Brassica napus])

HSP 1 Score: 283.9 bits (725), Expect = 1.9e-73
Identity = 142/165 (86.06%), Postives = 157/165 (95.15%), Query Frame = 1 

Query: 5   VKIARAFGASHIIAVDVQDDKLLKAKTIGATHTINSRKEDAIEKIREITGGMGVDVAVEA 64
           ++IARAFGAS IIAVDVQDDKL KAKT+GATHT+N+ KEDA+E+IREITGGMGVDVAVEA
Sbjct: 270 LQIARAFGASDIIAVDVQDDKLEKAKTLGATHTVNAAKEDAVERIREITGGMGVDVAVEA 329

Query: 65  LGRPQTFMQCTQSVKDGGKAVMIGLAQAGSVGEIDINRLVRRKIKVIGSYGGRARQDLPK 124
           LGRPQTFMQCT SVKDGGKAVMIGL+QAGSVGEIDINRLVRRKIKVIGSYGGRARQDLPK
Sbjct: 330 LGRPQTFMQCTLSVKDGGKAVMIGLSQAGSVGEIDINRLVRRKIKVIGSYGGRARQDLPK 389

Query: 125 LVKLAESGIFNLSDAVSRKYKFEDSNQAFQDLNEGKIIGRAVIEI 170
           +VKLAESGIFNL++AVS KYKFED+ +AFQDLNEGKI+ R V+EI
Sbjct: 390 VVKLAESGIFNLTNAVSSKYKFEDAGKAFQDLNEGKIVSRGVVEI 434

[Close]
BLAST of CSPI04G16940 vs. NCBI nr
Match: gi|922431464|ref|XP_013621826.1| (PREDICTED: alcohol dehydrogenase [Brassica oleracea var. oleracea])

HSP 1 Score: 283.5 bits (724), Expect = 2.5e-73
Identity = 142/165 (86.06%), Postives = 156/165 (94.55%), Query Frame = 1 

Query: 5   VKIARAFGASHIIAVDVQDDKLLKAKTIGATHTINSRKEDAIEKIREITGGMGVDVAVEA 64
           ++IARAFGAS IIAVDVQDDKL KAKT+GATHT+N+ KEDA+E+IREITGGMGVDVAVEA
Sbjct: 270 LQIARAFGASDIIAVDVQDDKLEKAKTLGATHTVNAAKEDAVERIREITGGMGVDVAVEA 329

Query: 65  LGRPQTFMQCTQSVKDGGKAVMIGLAQAGSVGEIDINRLVRRKIKVIGSYGGRARQDLPK 124
           LGRPQTFMQCT SVKDGGKAVMIGL+QAGSVGEIDINRLVRRKIKVIGSYGGRARQDLPK
Sbjct: 330 LGRPQTFMQCTLSVKDGGKAVMIGLSQAGSVGEIDINRLVRRKIKVIGSYGGRARQDLPK 389

Query: 125 LVKLAESGIFNLSDAVSRKYKFEDSNQAFQDLNEGKIIGRAVIEI 170
           +VKLAESGIFNL +AVS KYKFED+ +AFQDLNEGKI+ R V+EI
Sbjct: 390 VVKLAESGIFNLKNAVSSKYKFEDAGKAFQDLNEGKIVSRGVVEI 434

The following BLAST results are available for this feature:
BLAST of CSPI04G16940 vs. Swiss-Prot
Match NameE-valueIdentityDescription
SUCD_METS52.3e-2639.88Succinate-semialdehyde dehydrogenase (acetylating) OS=Metallosphaera sedula (str... [more]
DHSO_BACSU1.5e-1734.67Sorbitol dehydrogenase OS=Bacillus subtilis (strain 168) GN=gutB PE=1 SV=3[more]
TDH_CHRVO2.5e-1730.18L-threonine 3-dehydrogenase OS=Chromobacterium violaceum (strain ATCC 12472 / DS... [more]
FADH_AMYME5.6e-1731.48S-(hydroxymethyl)mycothiol dehydrogenase OS=Amycolatopsis methanolica PE=1 SV=2[more]
TDH_BACVZ1.4e-1532.05L-threonine 3-dehydrogenase OS=Bacillus velezensis (strain DSM 23117 / BGSC 10A6... [more]
BLAST of CSPI04G16940 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0KXY5_CUCSA6.1e-87100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_4G337910 PE=4 SV=1[more]
A0A078D5J5_BRANA1.3e-7386.06BnaC03g50730D protein OS=Brassica napus GN=BnaC03g50730D PE=4 SV=1[more]
A0A0D3BFD4_BRAOL1.7e-7386.06Uncharacterized protein (Fragment) OS=Brassica oleracea var. oleracea PE=4 SV=1[more]
A0A078HW01_BRANA3.8e-7385.45BnaA06g22770D protein OS=Brassica napus GN=BnaA06g22770D PE=4 SV=1[more]
M4FC33_BRARP3.8e-7385.45Uncharacterized protein OS=Brassica rapa subsp. pekinensis PE=4 SV=1[more]
BLAST of CSPI04G16940 vs. TAIR10
Match NameE-valueIdentityDescription
AT5G63620.15.6e-7684.85 GroES-like zinc-binding alcohol dehydrogenase family protein[more]
AT1G32780.13.4e-1232.34 GroES-like zinc-binding dehydrogenase family protein[more]
AT5G42250.14.4e-1233.54 Zinc-binding alcohol dehydrogenase family protein[more]
AT1G64710.15.7e-1230.19 GroES-like zinc-binding dehydrogenase family protein[more]
AT4G22110.17.5e-1235.19 GroES-like zinc-binding dehydrogenase family protein[more]
BLAST of CSPI04G16940 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|700199327|gb|KGN54485.1|8.7e-87100.00hypothetical protein Csa_4G337910 [Cucumis sativus][more]
gi|449449817|ref|XP_004142661.1|6.9e-8498.80PREDICTED: alcohol dehydrogenase 1B [Cucumis sativus][more]
gi|659069355|ref|XP_008449387.1|8.4e-8296.97PREDICTED: alcohol dehydrogenase 1B [Cucumis melo][more]
gi|674949008|emb|CDX84427.1|1.9e-7386.06BnaC03g50730D [Brassica napus][more]
gi|922431464|ref|XP_013621826.1|2.5e-7386.06PREDICTED: alcohol dehydrogenase [Brassica oleracea var. oleracea][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR002085Alcohol dehydrogenase superfamily, zinc-type
IPR011032GroES-like_sf
IPR013149ADH_C
IPR016040NAD(P)-bd_dom
Vocabulary: Molecular Function
TermDefinition
GO:0008270zinc ion binding
GO:0016491oxidoreductase activity
Vocabulary: Biological Process
TermDefinition
GO:0055114oxidation-reduction process
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055114 oxidation-reduction process
cellular_component GO:0005575 cellular_component
molecular_function GO:0016491 oxidoreductase activity
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0050897 cobalt ion binding
molecular_function GO:0051903 S-(hydroxymethyl)glutathione dehydrogenase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI04G16940.1CSPI04G16940.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002085Alcohol dehydrogenase superfamily, zinc-typePANTHERPTHR11695ALCOHOL DEHYDROGENASE RELATEDcoord: 7..170
score: 9.2E
IPR011032GroES-likeunknownSSF50129GroES-likecoord: 118..164
score: 3.1
IPR013149Alcohol dehydrogenase, C-terminalPFAMPF00107ADH_zinc_Ncoord: 4..129
score: 6.5
IPR016040NAD(P)-binding domainGENE3DG3DSA:3.40.50.720coord: 4..114
score: 3.4
IPR016040NAD(P)-binding domainunknownSSF51735NAD(P)-binding Rossmann-fold domainscoord: 4..134
score: 1.58
NoneNo IPR availablePANTHERPTHR11695:SF555GROES-LIKE ZINC-BINDING ALCOHOL DEHYDROGENASE FAMILY PROTEINcoord: 7..170
score: 9.2E

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
CSPI04G16940Csa4G337910Cucumber (Chinese Long) v2cpicuB181
CSPI04G16940Lsi01G020180Bottle gourd (USVL1VR-Ls)cpilsiB265
CSPI04G16940CsaV3_4G027540Cucumber (Chinese Long) v3cpicucB212
CSPI04G16940Cucsa.017880Cucumber (Gy14) v1cgycpiB400
CSPI04G16940CsGy4G016240Cucumber (Gy14) v2cgybcpiB170
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
CSPI04G16940Watermelon (97103) v1cpiwmB351