CSPI04G10300 (gene) Wild cucumber (PI 183967)

Overview
NameCSPI04G10300
Typegene
OrganismCucumis sativus (Wild cucumber (PI 183967))
DescriptionRetrovirus-related Pol polyprotein from transposon TNT 1-94
LocationChr4 : 8367200 .. 8368290 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTGTGAAGAATTCAATGCTCTTCAAGCACAAGGTACTTGGTCTTTAGTCCTTTGCCATCCATCCATGAATATTGTTGGTTGTAAGTGGGTCTTTCGCACCAAATATAATCATGATGGCTCAATTGCTCGCCACAAAGCACGCCTAGTTGCCAAAGGGTATCACCAGGTACAAGGTTTTGATTTTGATGAAACTTTTGTCATGTTGTCGAAAGGTCTACTATACAAATTATTCTTGCTCTAGCTGCTCAATACAATTGGTCATTGACACATTTAGATGTCAAAAATGCCCTCTTCATGGTGTTTTTCGGGAAACAGTATATATCTCTCAACCTACTTGTTTTCATGACAAAAAATTTCCAAACCATATGTGTCTTCTACACAAGAGTCTTTATGGCTTCAAACAAGCCCCTCGTACTTGGTTTGAACGCTTTACCTCTTATCTATTTACTCTGGGTTCTGTTGCTTCAAAGGTTGATCCATCATTATTCATTTGATATGTTGGATCTTCTTTTACTTACTTACTCATGTATGTTTATGATATTATTGTCAATGGCCCAAACTCCTCTTATATTTCTCTTCTAAAAAACCAATTGGCACTTGTTCAAAATATCTGATCTTGGTTCTTTAAAGTATTTTCTTGGTTTCGAAATACAATTCTCCACTAATGGCATTTTTGTCAACCAAGCAACATATCTCAATGATCTCCTTCACACATCAGAAATGACCTCTGTAAAATCTTGCACAACACCAATGTCTACCTCTCTTGACCTCTTCACCAGTGCGCCCCCTTTTAATGATCCCTTCTTTATACCGCAAAATTGTTGGTTCCTTACAATATCTTACATTTACTCACTTGATATATCACTTTCTCCGTAAATCGTGTTAGCCAATTCATGCACAAACCAACAATCATTCATTTTTCTGCAGTCAAACGCATCCTTCAATATTTATGTGGTACTCCCACCCTTGGAGTACATTTTCTCAAAGGTTCGTTCTCTCTCCAAACCTTTTGTGACTCTGATTGGGTAGGTGATACTTCTTACCGATGATCCACCTCAGGATTCATTGCCTTCATCGGCTCTAATCTAG

mRNA sequence

ATGTGTGAAGAATTCAATGCTCTTCAAGCACAAGGTACTTGGTCTTTAGTCCTTTGCCATCCATCCATGAATATTGTTGGTTGTAAGTGGGTCTTTCGCACCAAATATAATCATGATGGCTCAATTGCTCGCCACAAAGCACGCCTAGTTGCCAAAGGGTATCACCAGAGTCTTTATGGCTTCAAACAAGCCCCTCGTACTTGGTTTGAACGCTTTACCTCTTATCTATTTACTCTGGGTTCTGTTGCTTCAAAGTCAAACGCATCCTTCAATATTTATGTGGTACTCCCACCCTTGGAGTACATTTTCTCAAAGGTGATACTTCTTACCGATGATCCACCTCAGGATTCATTGCCTTCATCGGCTCTAATCTAG

Coding sequence (CDS)

ATGTGTGAAGAATTCAATGCTCTTCAAGCACAAGGTACTTGGTCTTTAGTCCTTTGCCATCCATCCATGAATATTGTTGGTTGTAAGTGGGTCTTTCGCACCAAATATAATCATGATGGCTCAATTGCTCGCCACAAAGCACGCCTAGTTGCCAAAGGGTATCACCAGAGTCTTTATGGCTTCAAACAAGCCCCTCGTACTTGGTTTGAACGCTTTACCTCTTATCTATTTACTCTGGGTTCTGTTGCTTCAAAGTCAAACGCATCCTTCAATATTTATGTGGTACTCCCACCCTTGGAGTACATTTTCTCAAAGGTGATACTTCTTACCGATGATCCACCTCAGGATTCATTGCCTTCATCGGCTCTAATCTAG
Homology
[Close]
BLAST of CSPI04G10300 vs. Swiss-Prot
Match: M820_ARATH (Uncharacterized mitochondrial protein AtMg00820 OS=Arabidopsis thaliana GN=AtMg00820 PE=4 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 5.7e-11
Identity = 32/56 (57.14%), Postives = 40/56 (71.43%), Query Frame = 1 

Query: 1  MCEEFNALQAQGTWSLVLCHPSMNIVGCKWVFRTKYNHDGSIARHKARLVAKGYHQ 57
          M EE +AL    TW LV    + NI+GCKWVF+TK + DG++ R KARLVAKG+HQ
Sbjct: 44 MQEELDALSRNKTWILVPPPVNQNILGCKWVFKTKLHSDGTLDRLKARLVAKGFHQ 99

[Close]
BLAST of CSPI04G10300 vs. Swiss-Prot
Match: POLX_TOBAC (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 52.8 bits (125), Expect = 3.2e-06
Identity = 25/56 (44.64%), Postives = 33/56 (58.93%), Query Frame = 1 

Query: 1   MCEEFNALQAQGTWSLVLCHPSMNIVGCKWVFRTKYNHDGSIARHKARLVAKGYHQ 57
           M EE  +LQ  GT+ LV        + CKWVF+ K + D  + R+KARLV KG+ Q
Sbjct: 830 MQEEMESLQKNGTYKLVELPKGKRPLKCKWVFKLKKDGDCKLVRYKARLVVKGFEQ 885

[Close]
BLAST of CSPI04G10300 vs. TrEMBL
Match: A5AS05_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_013040 PE=4 SV=1)

HSP 1 Score: 88.2 bits (217), Expect = 7.7e-15
Identity = 46/106 (43.40%), Postives = 53/106 (50.00%), Query Frame = 1 

Query: 1   MCEEFNALQAQGTWSLVLCHPSMNIVGCKWVFRTKYNHDGSIARHKARLVAKGYHQ---- 60
           M EE  +LQ   TW LV C P  N+VGC+W++  KY  DGSI R KARLV KGY Q    
Sbjct: 259 MNEEMKSLQKNETWELVECPPGKNLVGCRWIYTVKYKADGSIERFKARLVVKGYTQTYGI 318

Query: 61  ----------------------SLYGFKQAPRTWFERFTSYLFTLG 81
                                 SLYG KQ+PR WF RFT  +   G
Sbjct: 319 DYIETFAPVAKFNIXRVCKLKKSLYGLKQSPRAWFGRFTKSMRAFG 364

[Close]
BLAST of CSPI04G10300 vs. TrEMBL
Match: A5C979_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_027911 PE=4 SV=1)

HSP 1 Score: 87.0 bits (214), Expect = 1.7e-14
Identity = 42/87 (48.28%), Postives = 50/87 (57.47%), Query Frame = 1 

Query: 3   EEFNALQAQGTWSLVLCHPSMNIVGCKWVFRTKYNHDGSIARHKARLVAKGYHQ------ 62
           EE +AL+  GTW +        +VGCKW+F  KYN DGS+ R  ARLVAKG+ Q      
Sbjct: 606 EEIHALEKNGTWEISELPVGKRLVGCKWIFTVKYNEDGSVNRFNARLVAKGFTQSYGIDY 665

Query: 63  ----------SLYGFKQAPRTWFERFT 74
                     SLYG KQ+PR WFERFT
Sbjct: 666 EETFAPVAKRSLYGLKQSPRAWFERFT 692

[Close]
BLAST of CSPI04G10300 vs. TrEMBL
Match: A5BRC3_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_027576 PE=4 SV=1)

HSP 1 Score: 85.9 bits (211), Expect = 3.8e-14
Identity = 38/56 (67.86%), Postives = 44/56 (78.57%), Query Frame = 1 

Query: 1   MCEEFNALQAQGTWSLVLCHPSMNIVGCKWVFRTKYNHDGSIARHKARLVAKGYHQ 57
           M EE++AL   GTW LV  +PS N+VGCKW+FRTK N DGSI R KARLVAKG+HQ
Sbjct: 672 MSEEYDALVRNGTWELVPSNPSQNVVGCKWIFRTKRNSDGSIDRFKARLVAKGFHQ 727

[Close]
BLAST of CSPI04G10300 vs. TrEMBL
Match: Q25AL5_ORYSA (H0102C09.1 protein OS=Oryza sativa GN=H0102C09.1 PE=4 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 1.1e-13
Identity = 45/109 (41.28%), Postives = 59/109 (54.13%), Query Frame = 1 

Query: 1    MCEEFNALQAQGTWSLVLCHPSMNIVGCKWVFRTKYNHDGSIARHKARLVAKGYHQ---- 60
            M  E+ AL    TW LV    + NI+ CKWV + K   DGS+ R+KARLVAKG+ Q    
Sbjct: 897  MDAEYEALMGNKTWHLVPAEKANNIIDCKWVCKIKRKADGSLDRYKARLVAKGFKQRYGI 956

Query: 61   ------------SLYGFKQAPRTWFERFTSYLFTLGSVASKSNASFNIY 94
                        +LYG KQAPR W+ R ++ L  LG + SK++ S   Y
Sbjct: 957  NYEDTFSPVLDKALYGLKQAPRAWYSRLSTKLQELGFMPSKADTSLFFY 1005

[Close]
BLAST of CSPI04G10300 vs. TrEMBL
Match: G0XZB5_MALDO (Putative retroelement polyprotein OS=Malus domestica PE=4 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 1.1e-13
Identity = 42/115 (36.52%), Postives = 62/115 (53.91%), Query Frame = 1 

Query: 1   MCEEFNALQAQGTWSLVLCHPSMNIVGCKWVFRTKYNHDGSIARHKARLVAKGY------ 60
           M EE  AL    TWS+      M  VGC+WV++TK+  DG + RHKARLVA+G+      
Sbjct: 821 MDEELRALNENKTWSITKLPKGMRAVGCRWVYKTKFKSDGLVERHKARLVARGFTQTYGI 880

Query: 61  ---------------HQSLYGFKQAPRTWFERFTSYLFTLGSVASKSNASFNIYV 95
                          H+++YG KQ+PR W+ + +S L  +G    +SNA  +++V
Sbjct: 881 DYKETFAPVAKMNSLHKAIYGLKQSPRAWYAKLSSVLENIG--FKRSNADSSLFV 933

[Close]
BLAST of CSPI04G10300 vs. TAIR10
Match: ATMG00820.1 (ATMG00820.1 Reverse transcriptase (RNA-dependent DNA polymerase))

HSP 1 Score: 68.6 bits (166), Expect = 3.2e-12
Identity = 32/56 (57.14%), Postives = 40/56 (71.43%), Query Frame = 1 

Query: 1  MCEEFNALQAQGTWSLVLCHPSMNIVGCKWVFRTKYNHDGSIARHKARLVAKGYHQ 57
          M EE +AL    TW LV    + NI+GCKWVF+TK + DG++ R KARLVAKG+HQ
Sbjct: 44 MQEELDALSRNKTWILVPPPVNQNILGCKWVFKTKLHSDGTLDRLKARLVAKGFHQ 99

[Close]
BLAST of CSPI04G10300 vs. TAIR10
Match: AT4G23160.1 (AT4G23160.1 cysteine-rich RLK (RECEPTOR-like protein kinase) 8)

HSP 1 Score: 67.8 bits (164), Expect = 5.5e-12
Identity = 28/56 (50.00%), Postives = 38/56 (67.86%), Query Frame = 1 

Query: 1   MCEEFNALQAQGTWSLVLCHPSMNIVGCKWVFRTKYNHDGSIARHKARLVAKGYHQ 57
           M +E  A++   TW +    P+   +GCKWV++ KYN DG+I R+KARLVAKGY Q
Sbjct: 102 MDDEIGAMETTHTWEICTLPPNKKPIGCKWVYKIKYNSDGTIERYKARLVAKGYTQ 157

[Close]
BLAST of CSPI04G10300 vs. NCBI nr
Match: gi|645245491|ref|XP_008228903.1| (PREDICTED: uncharacterized protein LOC103328294 [Prunus mume])

HSP 1 Score: 90.5 bits (223), Expect = 2.2e-15
Identity = 50/107 (46.73%), Postives = 63/107 (58.88%), Query Frame = 1 

Query: 1   MCEEFNALQAQGTWSLVLCHPSMNIVGCKWVFRTKYNHDGSIARHKARLVAKGYHQSL-- 60
           M EE NAL  QGTWSLV    + N+VGCKW+F+ K + DGSIARHKARLVAKG+ Q    
Sbjct: 781 MKEEVNALHTQGTWSLVQLPTNKNLVGCKWIFKIKRHSDGSIARHKARLVAKGFSQEPGL 840

Query: 61  -YG---------------FKQAPRTWFERFTSYLFTLGSVASKSNAS 90
            YG                  +PR W +RFT +L +LG   + S++S
Sbjct: 841 DYGETFSPAVKPTTVRSNSSSSPRAWNDRFTQFLPSLGFENTYSDSS 887

[Close]
BLAST of CSPI04G10300 vs. NCBI nr
Match: gi|147857538|emb|CAN82537.1| (hypothetical protein VITISV_013040 [Vitis vinifera])

HSP 1 Score: 88.2 bits (217), Expect = 1.1e-14
Identity = 46/106 (43.40%), Postives = 53/106 (50.00%), Query Frame = 1 

Query: 1   MCEEFNALQAQGTWSLVLCHPSMNIVGCKWVFRTKYNHDGSIARHKARLVAKGYHQ---- 60
           M EE  +LQ   TW LV C P  N+VGC+W++  KY  DGSI R KARLV KGY Q    
Sbjct: 259 MNEEMKSLQKNETWELVECPPGKNLVGCRWIYTVKYKADGSIERFKARLVVKGYTQTYGI 318

Query: 61  ----------------------SLYGFKQAPRTWFERFTSYLFTLG 81
                                 SLYG KQ+PR WF RFT  +   G
Sbjct: 319 DYIETFAPVAKFNIXRVCKLKKSLYGLKQSPRAWFGRFTKSMRAFG 364

[Close]
BLAST of CSPI04G10300 vs. NCBI nr
Match: gi|802600351|ref|XP_012072900.1| (PREDICTED: uncharacterized protein LOC105634630 [Jatropha curcas])

HSP 1 Score: 87.4 bits (215), Expect = 1.9e-14
Identity = 50/130 (38.46%), Postives = 63/130 (48.46%), Query Frame = 1 

Query: 1   MCEEFNALQAQGTWSLVLCHPSMNIVGCKWVFRTKYNHDGSIARHKARLVAKGYHQ---- 60
           M +E+NAL + GTW LV  + + N VGCKWVFR K N DGSIA++KARLVAKGY Q    
Sbjct: 503 MSDEYNALISNGTWLLVPPNRNQNFVGCKWVFRLKKNLDGSIAKYKARLVAKGYSQWPRV 562

Query: 61  ---------------------------------SLYGFKQAPRTWFERFTSYLFTLGSVA 94
                                            +LYG +QAPR W++         G V 
Sbjct: 563 DFEETFSPEEVYMQQPPRFVDSSKPTFVCKLVKALYGLRQAPRAWYQALRKCALDFGFVQ 622

[Close]
BLAST of CSPI04G10300 vs. NCBI nr
Match: gi|147841808|emb|CAN75434.1| (hypothetical protein VITISV_027911 [Vitis vinifera])

HSP 1 Score: 87.0 bits (214), Expect = 2.5e-14
Identity = 42/87 (48.28%), Postives = 50/87 (57.47%), Query Frame = 1 

Query: 3   EEFNALQAQGTWSLVLCHPSMNIVGCKWVFRTKYNHDGSIARHKARLVAKGYHQ------ 62
           EE +AL+  GTW +        +VGCKW+F  KYN DGS+ R  ARLVAKG+ Q      
Sbjct: 606 EEIHALEKNGTWEISELPVGKRLVGCKWIFTVKYNEDGSVNRFNARLVAKGFTQSYGIDY 665

Query: 63  ----------SLYGFKQAPRTWFERFT 74
                     SLYG KQ+PR WFERFT
Sbjct: 666 EETFAPVAKRSLYGLKQSPRAWFERFT 692

[Close]
BLAST of CSPI04G10300 vs. NCBI nr
Match: gi|694433789|ref|XP_009344163.1| (PREDICTED: uncharacterized protein LOC103936058 [Pyrus x bretschneideri])

HSP 1 Score: 86.7 bits (213), Expect = 3.2e-14
Identity = 38/56 (67.86%), Postives = 43/56 (76.79%), Query Frame = 1 

Query: 1  MCEEFNALQAQGTWSLVLCHPSMNIVGCKWVFRTKYNHDGSIARHKARLVAKGYHQ 57
          M  EF ALQ  GTW LV  HPS N+VGCKWVF+ K+  DGSI R+KARLVAKG+HQ
Sbjct: 1  MANEFAALQTTGTWELVPSHPSYNLVGCKWVFKVKHKADGSIERYKARLVAKGFHQ 56

The following BLAST results are available for this feature:
BLAST of CSPI04G10300 vs. Swiss-Prot
Match NameE-valueIdentityDescription
M820_ARATH5.7e-1157.14Uncharacterized mitochondrial protein AtMg00820 OS=Arabidopsis thaliana GN=AtMg0... [more]
POLX_TOBAC3.2e-0644.64Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum... [more]
BLAST of CSPI04G10300 vs. TrEMBL
Match NameE-valueIdentityDescription
A5AS05_VITVI7.7e-1543.40Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_013040 PE=4 SV=1[more]
A5C979_VITVI1.7e-1448.28Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_027911 PE=4 SV=1[more]
A5BRC3_VITVI3.8e-1467.86Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_027576 PE=4 SV=1[more]
Q25AL5_ORYSA1.1e-1341.28H0102C09.1 protein OS=Oryza sativa GN=H0102C09.1 PE=4 SV=1[more]
G0XZB5_MALDO1.1e-1336.52Putative retroelement polyprotein OS=Malus domestica PE=4 SV=1[more]
BLAST of CSPI04G10300 vs. TAIR10
Match NameE-valueIdentityDescription
ATMG00820.13.2e-1257.14ATMG00820.1 Reverse transcriptase (RNA-dependent DNA polymerase)[more]
AT4G23160.15.5e-1250.00 cysteine-rich RLK (RECEPTOR-like protein kinase) 8[more]
BLAST of CSPI04G10300 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|645245491|ref|XP_008228903.1|2.2e-1546.73PREDICTED: uncharacterized protein LOC103328294 [Prunus mume][more]
gi|147857538|emb|CAN82537.1|1.1e-1443.40hypothetical protein VITISV_013040 [Vitis vinifera][more]
gi|802600351|ref|XP_012072900.1|1.9e-1438.46PREDICTED: uncharacterized protein LOC105634630 [Jatropha curcas][more]
gi|147841808|emb|CAN75434.1|2.5e-1448.28hypothetical protein VITISV_027911 [Vitis vinifera][more]
gi|694433789|ref|XP_009344163.1|3.2e-1467.86PREDICTED: uncharacterized protein LOC103936058 [Pyrus x bretschneideri][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR013103RVT_2
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0005488 binding
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI04G10300.1CSPI04G10300.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013103Reverse transcriptase, RNA-dependent DNA polymerasePFAMPF07727RVT_2coord: 12..56
score: 5.4
NoneNo IPR availablePANTHERPTHR11439GAG-POL-RELATED RETROTRANSPOSONcoord: 1..89
score: 4.8
NoneNo IPR availablePANTHERPTHR11439:SF185SUBFAMILY NOT NAMEDcoord: 1..89
score: 4.8

Synteny
The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
CSPI04G10300Wax gourdcpiwgoB375
CSPI04G10300Cucurbita maxima (Rimu)cmacpiB265
CSPI04G10300Cucurbita moschata (Rifu)cmocpiB252
CSPI04G10300Cucumber (Chinese Long) v2cpicuB182
CSPI04G10300Melon (DHL92) v3.5.1cpimeB313
CSPI04G10300Melon (DHL92) v3.5.1cpimeB332
CSPI04G10300Watermelon (Charleston Gray)cpiwcgB347
CSPI04G10300Watermelon (97103) v1cpiwmB312
CSPI04G10300Cucurbita pepo (Zucchini)cpecpiB619
CSPI04G10300Melon (DHL92) v3.6.1cpimedB304
CSPI04G10300Cucumber (Gy14) v2cgybcpiB168
CSPI04G10300Cucumber (Chinese Long) v3cpicucB213