CSPI04G05160 (gene) Wild cucumber (PI 183967) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CSPI04G05160
Type	gene
Organism	Cucumis sativus (Wild cucumber (PI 183967))
Description	Cytochrome P450 family ferulate hydroxylase
Location	Chr4 : 3488991 .. 3489870 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GACTCAATTAATATATATCTATTATTTCATATCAACTTCTACTTCAAACGTAGTACCGATCATGTAAGTCCATGATACGTGTCTGTGCTGATAAAACACTTTTCTTCAACAATGTATTGATGTAATGTTACATCAATCAATTAACAGAATTATAATAATAATAACAATATTATTATTATTGAAATCAAAACTCCAATAATTGCAAATATTTCACAATTTTCAGGATGTGATGTTCGGTGGCACAGAGACAGTGGCCTCCGCCATAGAATGGACCATGGCTGAGCTCATGAAAAACCCCGACGACCTCGAAAAGCTTCAACAGCAACTCGACGACACCGTCGGCCTTCACCACCGTCCACAAGAATCCGACTTACACAATCTAACATACCTCAGATGCATTCTGAAAGAAACTCTCCGTCTCCACCCGCCGATTCCGGTGCTCCTCCACGAAACGGCAGCCGAAACCGAACTCGCCCGCTCTCGGATTCCTGCCAAGTCTCGTGTCTTCATCAACGCATGGGCGATCGGTCGGGACGAGAAGTCCCGGGTGGACCCCGATATTTTCCGGCCGGCGAGGTTTGAAATCGAAGGTGCGGCGGATTTTAAAGGTGGGGACTTCGAGTTTATTCCGTTCGGGTCAGGGCGGAGGTCTTGCCCTGGAATGTAGCTGGGGGTTTATGCGGTGGAGATGGCGGTGGCGAATTTAGGGTGATCAACATGTTAATAAATAAAACATTTTTTTTAAAGAAAACTAAAATTAAAGTTTATTCAAAATATATAAACGTTAGGATTAGACATTTAAATTACAAGAGAACCAAAACTTCAAAATTTAGAACCTAAATGATATTTTTTGACCAATATGTATTAATTATTGTTTGGT

mRNA sequence

ATGTTCGGTGGCACAGAGACAGTGGCCTCCGCCATAGAATGGACCATGGCTGAGCTCATGAAAAACCCCGACGACCTCGAAAAGCTTCAACAGCAACTCGACGACACCGTCGGCCTTCACCACCGTCCACAAGAATCCGACTTACACAATCTAACATACCTCAGATGCATTCTGAAAGAAACTCTCCGTCTCCACCCGCCGATTCCGGTGCTCCTCCACGAAACGGCAGCCGAAACCGAACTCGCCCGCTCTCGGATTCCTGCCAAGTCTCGTGTCTTCATCAACGCATGGGCGATCGGTCGGGACGAGAAGTCCCGGGTGGACCCCGATATTTTCCGGCCGGCGAGGTTTGAAATCGAAGGTGCGGCGGATTTTAAAGGTGGGGACTTCGAGTTTATTCCGTTCGGGTCAGGGCGGAGGTCTTGCCCTGGAATGTAG

Coding sequence (CDS)

ATGTTCGGTGGCACAGAGACAGTGGCCTCCGCCATAGAATGGACCATGGCTGAGCTCATGAAAAACCCCGACGACCTCGAAAAGCTTCAACAGCAACTCGACGACACCGTCGGCCTTCACCACCGTCCACAAGAATCCGACTTACACAATCTAACATACCTCAGATGCATTCTGAAAGAAACTCTCCGTCTCCACCCGCCGATTCCGGTGCTCCTCCACGAAACGGCAGCCGAAACCGAACTCGCCCGCTCTCGGATTCCTGCCAAGTCTCGTGTCTTCATCAACGCATGGGCGATCGGTCGGGACGAGAAGTCCCGGGTGGACCCCGATATTTTCCGGCCGGCGAGGTTTGAAATCGAAGGTGCGGCGGATTTTAAAGGTGGGGACTTCGAGTTTATTCCGTTCGGGTCAGGGCGGAGGTCTTGCCCTGGAATGTAG

Homology

[Close]

BLAST of CSPI04G05160 vs. Swiss-Prot
Match: C84A1_ARATH (Cytochrome P450 84A1 OS=Arabidopsis thaliana GN=CYP84A1 PE=1 SV=1)

HSP 1 Score: 203.0 bits (515), Expect = 2.3e-51
Identity = 96/145 (66.21%), Postives = 116/145 (80.00%), Query Frame = 1


Query: 1   MFGGTETVASAIEWTMAELMKNPDDLEKLQQQLDDTVGLHHRPQESDLHNLTYLRCILKE 60
           MFGGTETVASAIEW + EL+++P+DL+++QQ+L + VGL  R +ESD+  LTYL+C LKE
Sbjct: 317 MFGGTETVASAIEWALTELLRSPEDLKRVQQELAEVVGLDRRVEESDIEKLTYLKCTLKE 376

Query: 61  TLRLHPPIPVLLHETAAETELARSRIPAKSRVFINAWAIGRDEKSRVDPDIFRPARFEIE 120
           TLR+HPPIP+LLHETA +T +    IP KSRV INA+AIGRD  S  DPD FRP+RF   
Sbjct: 377 TLRMHPPIPLLLHETAEDTSIDGFFIPKKSRVMINAFAIGRDPTSWTDPDTFRPSRFLEP 436

Query: 121 GAADFKGGDFEFIPFGSGRRSCPGM 146
           G  DFKG +FEFIPFGSGRRSCPGM
Sbjct: 437 GVPDFKGSNFEFIPFGSGRRSCPGM 461

[Close]

BLAST of CSPI04G05160 vs. Swiss-Prot
Match: C84A4_ARATH (Cytochrome P450 84A4 OS=Arabidopsis thaliana GN=CYP84A4 PE=1 SV=1)

HSP 1 Score: 172.6 bits (436), Expect = 3.3e-42
Identity = 81/146 (55.48%), Postives = 108/146 (73.97%), Query Frame = 1


Query: 1   MFGGTETVASAIEWTMAELMKNPDDLEKLQQQLDDTVGLHH-RPQESDLHNLTYLRCILK 60
           MFGGTETVA AIEW + E++++P++++++Q +L   VGL   R +++ L  LT+L+CILK
Sbjct: 305 MFGGTETVALAIEWVLTEILRSPENMKRVQDELTSVVGLDRWRVEDTHLEKLTFLKCILK 364

Query: 61  ETLRLHPPIPVLLHETAAETELARSRIPAKSRVFINAWAIGRDEKSRVDPDIFRPARFEI 120
           ETLRLHPP P+LLHET  +TE++   IP  SRV +N +A+GRD  S  DP+ F P RF  
Sbjct: 365 ETLRLHPPFPLLLHETVKDTEISGYFIPKGSRVMVNTYALGRDPNSWSDPESFNPGRFLN 424

Query: 121 EGAADFKGGDFEFIPFGSGRRSCPGM 146
             A D KG +FEF+PFGSGRRSCPGM
Sbjct: 425 PIAPDLKGNNFEFVPFGSGRRSCPGM 450

[Close]

BLAST of CSPI04G05160 vs. Swiss-Prot
Match: C71A1_PERAE (Cytochrome P450 71A1 OS=Persea americana GN=CYP71A1 PE=1 SV=2)

HSP 1 Score: 155.6 bits (392), Expect = 4.1e-37
Identity = 77/144 (53.47%), Postives = 101/144 (70.14%), Query Frame = 1


Query: 3   GGTETVASAIEWTMAELMKNPDDLEKLQQQLDDTVGLHHRPQESDLHNLTYLRCILKETL 62
           GGT+T A  +EW MAEL+K+PD +EK QQ++   VG   + +E DLH L YL+ I+KETL
Sbjct: 304 GGTDTTAVTLEWAMAELIKHPDVMEKAQQEVRRVVGKKAKVEEEDLHQLHYLKLIIKETL 363

Query: 63  RLHPPIPVLL-HETAAETELARSRIPAKSRVFINAWAIGRDEKSRVDPDIFRPARFEIEG 122
           RLHP  P+L+  E+  +  +    IPAK+RVFINAWAIGRD KS  + + F P RF +  
Sbjct: 364 RLHPVAPLLVPRESTRDVVIRGYHIPAKTRVFINAWAIGRDPKSWENAEEFLPERF-VNN 423

Query: 123 AADFKGGDFEFIPFGSGRRSCPGM 146
           + DFKG DF+ IPFG+GRR CPG+
Sbjct: 424 SVDFKGQDFQLIPFGAGRRGCPGI 446

[Close]

BLAST of CSPI04G05160 vs. Swiss-Prot
Match: C76B6_CATRO (Geraniol 8-hydroxylase OS=Catharanthus roseus GN=CYP76B6 PE=1 SV=1)

HSP 1 Score: 147.1 bits (370), Expect = 1.5e-34
Identity = 68/143 (47.55%), Postives = 96/143 (67.13%), Query Frame = 1


Query: 4   GTETVASAIEWTMAELMKNPDDLEKLQQQLDDTVGLHHRPQESDLHNLTYLRCILKETLR 63
           GT+T +S +EW M+E++KNPD ++K Q +L   +G     +ESD++ L YLRC++KETLR
Sbjct: 298 GTDTTSSTLEWAMSEMLKNPDKMKKTQDELAQVIGRGKTIEESDINRLPYLRCVMKETLR 357

Query: 64  LHPPIPVLL-HETAAETELARSRIPAKSRVFINAWAIGRDEKSRVDPDIFRPARFEIEGA 123
           +HPP+P L+  +     E+    +P  S+V +NAWAIGRDE    D   F+P RF +E  
Sbjct: 358 IHPPVPFLIPRKVEQSVEVCGYNVPKGSQVLVNAWAIGRDETVWDDALAFKPERF-MESE 417

Query: 124 ADFKGGDFEFIPFGSGRRSCPGM 146
            D +G DFE IPFG+GRR CPG+
Sbjct: 418 LDIRGRDFELIPFGAGRRICPGL 439

[Close]

BLAST of CSPI04G05160 vs. Swiss-Prot
Match: C71DI_MENGR (Cytochrome P450 71D18 OS=Mentha gracilis GN=CYP71D18 PE=1 SV=1)

HSP 1 Score: 145.2 bits (365), Expect = 5.6e-34
Identity = 70/142 (49.30%), Postives = 96/142 (67.61%), Query Frame = 1


Query: 4   GTETVASAIEWTMAELMKNPDDLEKLQQQLDDTVGLHHRPQESDLHNLTYLRCILKETLR 63
           G ET ++ I W ++ELM+NP  + K+Q ++ + +        S++  L YLR +LKETLR
Sbjct: 299 GAETSSTTISWALSELMRNPAKMAKVQAEVREALKGKTVVDLSEVQELKYLRSVLKETLR 358

Query: 64  LHPPIPVLLHETAAETELARSRIPAKSRVFINAWAIGRDEKSRVDPDIFRPARFEIEGAA 123
           LHPP P++  ++  E E+    IPAK+R+FIN WAIGRD +   DPD FRP RF+ E + 
Sbjct: 359 LHPPFPLIPRQSREECEVNGYTIPAKTRIFINVWAIGRDPQYWEDPDTFRPERFD-EVSR 418

Query: 124 DFKGGDFEFIPFGSGRRSCPGM 146
           DF G DFEFIPFG+GRR CPG+
Sbjct: 419 DFMGNDFEFIPFGAGRRICPGL 439

[Close]

BLAST of CSPI04G05160 vs. TrEMBL
Match: Q6PLI4_CENER (Coniferylalcohol 5-hydroxylase OS=Centaurium erythraea PE=2 SV=1)

HSP 1 Score: 228.4 bits (581), Expect = 5.6e-57
Identity = 109/145 (75.17%), Postives = 121/145 (83.45%), Query Frame = 1


Query: 1   MFGGTETVASAIEWTMAELMKNPDDLEKLQQQLDDTVGLHHRPQESDLHNLTYLRCILKE 60
           MFGGTETVASAIEW MAELM+NPDDL+++QQ+L D VGLH + QESD   LTY +C LKE
Sbjct: 315 MFGGTETVASAIEWAMAELMRNPDDLKRVQQELSDVVGLHRKVQESDFEKLTYFKCCLKE 374

Query: 61  TLRLHPPIPVLLHETAAETELARSRIPAKSRVFINAWAIGRDEKSRVDPDIFRPARFEIE 120
           TLRLHPPIP+LLHETA  +E+A  RIPAKSRV INAWAIGRD+ S  DPD FRPARF  E
Sbjct: 375 TLRLHPPIPLLLHETAEASEIAGYRIPAKSRVMINAWAIGRDKDSWKDPDTFRPARFLEE 434

Query: 121 GAADFKGGDFEFIPFGSGRRSCPGM 146
           G  DFKG +FEFIPFGSGRRSCPGM
Sbjct: 435 GVPDFKGSNFEFIPFGSGRRSCPGM 459

[Close]

BLAST of CSPI04G05160 vs. TrEMBL
Match: A0A059ASK6_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_I02371 PE=3 SV=1)

HSP 1 Score: 218.0 bits (554), Expect = 7.6e-54
Identity = 102/145 (70.34%), Postives = 121/145 (83.45%), Query Frame = 1


Query: 1   MFGGTETVASAIEWTMAELMKNPDDLEKLQQQLDDTVGLHHRPQESDLHNLTYLRCILKE 60
           MFGGTETVASAIEW M ELM++P+DL+K+Q++L D VGLH R +ESD   LTYL+C++KE
Sbjct: 320 MFGGTETVASAIEWAMTELMRSPEDLKKVQKELADVVGLHRRVEESDFEKLTYLKCVIKE 379

Query: 61  TLRLHPPIPVLLHETAAETELARSRIPAKSRVFINAWAIGRDEKSRVDPDIFRPARFEIE 120
           TLRLHPPIP+LLHETA + E++   IPAK+RV INAWAIGRD  S  DPD F+P+RF  E
Sbjct: 380 TLRLHPPIPLLLHETAEDAEVSGYFIPAKTRVMINAWAIGRDPTSWEDPDTFKPSRFLNE 439

Query: 121 GAADFKGGDFEFIPFGSGRRSCPGM 146
           GA DFKG +FEFIPFGSGRRSCPGM
Sbjct: 440 GAPDFKGSNFEFIPFGSGRRSCPGM 464

[Close]

BLAST of CSPI04G05160 vs. TrEMBL
Match: F6GWQ4_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_04s0023g02900 PE=3 SV=1)

HSP 1 Score: 217.2 bits (552), Expect = 1.3e-53
Identity = 102/145 (70.34%), Postives = 122/145 (84.14%), Query Frame = 1


Query: 1   MFGGTETVASAIEWTMAELMKNPDDLEKLQQQLDDTVGLHHRPQESDLHNLTYLRCILKE 60
           MFGGTETVASAIEW MAE+MK+P+DL+K+QQ+L D VGL+ R +ESDL  LTYL+C+LKE
Sbjct: 310 MFGGTETVASAIEWAMAEMMKSPEDLKKVQQELADVVGLNRRVEESDLEKLTYLKCVLKE 369

Query: 61  TLRLHPPIPVLLHETAAETELARSRIPAKSRVFINAWAIGRDEKSRVDPDIFRPARFEIE 120
           TLRLHPPIP+LLHETA + E+A   IPA+SRV INAWAIGRD+ S  +P+ F+P+RF   
Sbjct: 370 TLRLHPPIPLLLHETAEDAEVAGYHIPARSRVMINAWAIGRDKNSWDEPETFKPSRFLKA 429

Query: 121 GAADFKGGDFEFIPFGSGRRSCPGM 146
           G  DFKG +FEFIPFGSGRRSCPGM
Sbjct: 430 GVPDFKGSNFEFIPFGSGRRSCPGM 454

[Close]

BLAST of CSPI04G05160 vs. TrEMBL
Match: A5AK80_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_026695 PE=3 SV=1)

HSP 1 Score: 215.7 bits (548), Expect = 3.8e-53
Identity = 102/145 (70.34%), Postives = 121/145 (83.45%), Query Frame = 1


Query: 1   MFGGTETVASAIEWTMAELMKNPDDLEKLQQQLDDTVGLHHRPQESDLHNLTYLRCILKE 60
           MFGGTETVASAIEW MAE+MK+P+DL+K+QQ+L D VGL+ R +ESDL  LTYL+C LKE
Sbjct: 289 MFGGTETVASAIEWAMAEMMKSPEDLKKVQQELADVVGLNRRVEESDLEKLTYLKCALKE 348

Query: 61  TLRLHPPIPVLLHETAAETELARSRIPAKSRVFINAWAIGRDEKSRVDPDIFRPARFEIE 120
           TLRLHPPIP+LLHETA + E+A   IPA+SRV INAWAIGRD+ S  +P+ F+P+RF   
Sbjct: 349 TLRLHPPIPLLLHETAEDAEVAGYHIPARSRVMINAWAIGRDKNSWDEPETFKPSRFLKA 408

Query: 121 GAADFKGGDFEFIPFGSGRRSCPGM 146
           G  DFKG +FEFIPFGSGRRSCPGM
Sbjct: 409 GVPDFKGSNFEFIPFGSGRRSCPGM 433

[Close]

BLAST of CSPI04G05160 vs. TrEMBL
Match: A0A088B182_9ROSI (Putative ferulate 5-hydroxylase OS=Hibiscus cannabinus GN=F5H PE=2 SV=1)

HSP 1 Score: 215.3 bits (547), Expect = 4.9e-53
Identity = 101/145 (69.66%), Postives = 123/145 (84.83%), Query Frame = 1


Query: 1   MFGGTETVASAIEWTMAELMKNPDDLEKLQQQLDDTVGLHHRPQESDLHNLTYLRCILKE 60
           MFGGTETVASAIEW ++ELMK+P+D++++QQ+L + VGL  R +ESD+  LT+L+C LKE
Sbjct: 314 MFGGTETVASAIEWALSELMKSPEDMKRVQQELAEVVGLDRRVEESDIEKLTFLKCTLKE 373

Query: 61  TLRLHPPIPVLLHETAAETELARSRIPAKSRVFINAWAIGRDEKSRVDPDIFRPARFEIE 120
           TLRLHPPIP+LLHETA +T +A  +IPAKSRV INAWAIGRD+ S  DPD F+P+RF  E
Sbjct: 374 TLRLHPPIPLLLHETAEDTVVAGYQIPAKSRVMINAWAIGRDKDSWEDPDSFKPSRFLKE 433

Query: 121 GAADFKGGDFEFIPFGSGRRSCPGM 146
           G ADFKG +FEFIPFGSGRRSCPGM
Sbjct: 434 GVADFKGSNFEFIPFGSGRRSCPGM 458

[Close]

BLAST of CSPI04G05160 vs. TAIR10
Match: AT4G36220.1 (AT4G36220.1 ferulic acid 5-hydroxylase 1)

HSP 1 Score: 203.0 bits (515), Expect = 1.3e-52
Identity = 96/145 (66.21%), Postives = 116/145 (80.00%), Query Frame = 1


Query: 1   MFGGTETVASAIEWTMAELMKNPDDLEKLQQQLDDTVGLHHRPQESDLHNLTYLRCILKE 60
           MFGGTETVASAIEW + EL+++P+DL+++QQ+L + VGL  R +ESD+  LTYL+C LKE
Sbjct: 317 MFGGTETVASAIEWALTELLRSPEDLKRVQQELAEVVGLDRRVEESDIEKLTYLKCTLKE 376

Query: 61  TLRLHPPIPVLLHETAAETELARSRIPAKSRVFINAWAIGRDEKSRVDPDIFRPARFEIE 120
           TLR+HPPIP+LLHETA +T +    IP KSRV INA+AIGRD  S  DPD FRP+RF   
Sbjct: 377 TLRMHPPIPLLLHETAEDTSIDGFFIPKKSRVMINAFAIGRDPTSWTDPDTFRPSRFLEP 436

Query: 121 GAADFKGGDFEFIPFGSGRRSCPGM 146
           G  DFKG +FEFIPFGSGRRSCPGM
Sbjct: 437 GVPDFKGSNFEFIPFGSGRRSCPGM 461

[Close]

BLAST of CSPI04G05160 vs. TAIR10
Match: AT5G04330.1 (AT5G04330.1 Cytochrome P450 superfamily protein)

HSP 1 Score: 172.6 bits (436), Expect = 1.8e-43
Identity = 81/146 (55.48%), Postives = 108/146 (73.97%), Query Frame = 1


Query: 1   MFGGTETVASAIEWTMAELMKNPDDLEKLQQQLDDTVGLHH-RPQESDLHNLTYLRCILK 60
           MFGGTETVA AIEW + E++++P++++++Q +L   VGL   R +++ L  LT+L+CILK
Sbjct: 305 MFGGTETVALAIEWVLTEILRSPENMKRVQDELTSVVGLDRWRVEDTHLEKLTFLKCILK 364

Query: 61  ETLRLHPPIPVLLHETAAETELARSRIPAKSRVFINAWAIGRDEKSRVDPDIFRPARFEI 120
           ETLRLHPP P+LLHET  +TE++   IP  SRV +N +A+GRD  S  DP+ F P RF  
Sbjct: 365 ETLRLHPPFPLLLHETVKDTEISGYFIPKGSRVMVNTYALGRDPNSWSDPESFNPGRFLN 424

Query: 121 EGAADFKGGDFEFIPFGSGRRSCPGM 146
             A D KG +FEF+PFGSGRRSCPGM
Sbjct: 425 PIAPDLKGNNFEFVPFGSGRRSCPGM 450

[Close]

BLAST of CSPI04G05160 vs. TAIR10
Match: AT3G52970.2 (AT3G52970.2 cytochrome P450, family 76, subfamily G, polypeptide 1)

HSP 1 Score: 136.3 bits (342), Expect = 1.5e-32
Identity = 70/146 (47.95%), Postives = 94/146 (64.38%), Query Frame = 1


Query: 4   GTETVASAIEWTMAELMKNPDDLEKLQQQLDDTV-GLHHRPQESDLHNLTYLRCILKETL 63
           GT+T  S +EW +AEL+ NP  L KLQ +L       + + QE DL NL YL  ++ ETL
Sbjct: 328 GTDTTTSTLEWALAELLHNPRTLTKLQTELRTYFKSSNQKLQEEDLPNLPYLSAVIMETL 387

Query: 64  RLHPPIPVLLHETAAET--ELARSRIPAKSRVFINAWAIGRDEKSRVDPDIFRPARFEIE 123
           RLHPP+P L+   A  T     +  IP +++V +N WAIGRD K+ +DP +F+P RF  +
Sbjct: 388 RLHPPLPFLVPHKAMSTCHIFDQYTIPKETQVLVNVWAIGRDPKTWIDPIMFKPERFISD 447

Query: 124 -GAADFKGGDFEFIPFGSGRRSCPGM 146
             A DFKG D+EF+PFGSGRR CP +
Sbjct: 448 PDARDFKGQDYEFLPFGSGRRMCPAL 473

[Close]

BLAST of CSPI04G05160 vs. TAIR10
Match: AT5G06900.1 (AT5G06900.1 cytochrome P450, family 93, subfamily D, polypeptide 1)

HSP 1 Score: 135.2 bits (339), Expect = 3.3e-32
Identity = 66/142 (46.48%), Postives = 95/142 (66.90%), Query Frame = 1


Query: 3   GGTETVASAIEWTMAELMKNPDDLEKLQQQLDDTVGLHHRPQESDLHNLTYLRCILKETL 62
           GGT+T A  +EW +AEL+ +P+ ++K QQ+++  VG     +ESDL NL+Y + ++KET+
Sbjct: 304 GGTDTSAITVEWALAELINHPEIMKKAQQEIEQVVGNKRVVEESDLCNLSYTQAVVKETM 363

Query: 63  RLHPPIPVLLHETAAETELARSRIPAKSRVFINAWAIGRDEKSRVDPDIFRPARFEIEGA 122
           RLHP  P+ + E+  E  +A  RIPAK+RV +N WAIGRD     DP  FRP RFE    
Sbjct: 364 RLHPGGPIFVRESDEECAVAGFRIPAKTRVIVNVWAIGRDSNQWEDPLEFRPERFE---G 423

Query: 123 ADFKGGDFEFIPFGSGRRSCPG 145
           +++K    + + FG+GRRSCPG
Sbjct: 424 SEWKVMSEKMMSFGAGRRSCPG 442

[Close]

BLAST of CSPI04G05160 vs. TAIR10
Match: AT4G22710.1 (AT4G22710.1 cytochrome P450, family 706, subfamily A, polypeptide 2)

HSP 1 Score: 132.9 bits (333), Expect = 1.6e-31
Identity = 64/143 (44.76%), Postives = 97/143 (67.83%), Query Frame = 1


Query: 1   MFGGTETVASAIEWTMAELMKNPDDLEKLQQQLDDTVGLHHRPQESDLHNLTYLRCILKE 60
           + GG +T  +A E+ MAE++  P+ L K++ +LD  VG  +  +ES L  L YL+ ++KE
Sbjct: 322 VLGGVDTSVNASEFAMAEIVSRPEVLNKIRLELDQVVGKDNIVEESHLPKLPYLQAVMKE 381

Query: 61  TLRLHPPIPVLL-HETAAETELARSRIPAKSRVFINAWAIGRDEKSRVDPDIFRPARFEI 120
           TLRLHP +P+L+ H  +  + +A   +P  S++FIN WAI RD K+  +P+ F+P RF +
Sbjct: 382 TLRLHPTLPLLVPHRNSETSVVAGYTVPKDSKIFINVWAIHRDPKNWDEPNEFKPERF-L 441

Query: 121 EGAADFKGGDFEFIPFGSGRRSC 143
           E + DF GGDF+++PFGSGRR C
Sbjct: 442 ENSLDFNGGDFKYLPFGSGRRIC 463

[Close]

BLAST of CSPI04G05160 vs. NCBI nr
Match: gi|659107516|ref|XP_008453715.1| (PREDICTED: LOW QUALITY PROTEIN: cytochrome P450 84A1-like [Cucumis melo])

HSP 1 Score: 280.8 bits (717), Expect = 1.4e-72
Identity = 135/145 (93.10%), Postives = 141/145 (97.24%), Query Frame = 1


Query: 1   MFGGTETVASAIEWTMAELMKNPDDLEKLQQQLDDTVGLHHRPQESDLHNLTYLRCILKE 60
           MFGGTETVASAIEWTMAELM+NPDDL+KLQQQLD TVGLHHRPQESDLHNLTYLRCILKE
Sbjct: 313 MFGGTETVASAIEWTMAELMRNPDDLKKLQQQLDATVGLHHRPQESDLHNLTYLRCILKE 372

Query: 61  TLRLHPPIPVLLHETAAETELARSRIPAKSRVFINAWAIGRDEKSRVDPDIFRPARFEIE 120
           TLRLHPPIP+LLHETAA+T+LA  RIPAKSRVFINAWAIGRDEKS VDPDIFRPARFE+E
Sbjct: 373 TLRLHPPIPLLLHETAADTKLAGYRIPAKSRVFINAWAIGRDEKSWVDPDIFRPARFEME 432

Query: 121 GAADFKGGDFEFIPFGSGRRSCPGM 146
           GAADFKGGDFEFIPFGSGRRSCPGM
Sbjct: 433 GAADFKGGDFEFIPFGSGRRSCPGM 457

[Close]

BLAST of CSPI04G05160 vs. NCBI nr
Match: gi|46403211|gb|AAS92625.1| (coniferylalcohol 5-hydroxylase [Centaurium erythraea])

HSP 1 Score: 228.4 bits (581), Expect = 8.0e-57
Identity = 109/145 (75.17%), Postives = 121/145 (83.45%), Query Frame = 1


Query: 1   MFGGTETVASAIEWTMAELMKNPDDLEKLQQQLDDTVGLHHRPQESDLHNLTYLRCILKE 60
           MFGGTETVASAIEW MAELM+NPDDL+++QQ+L D VGLH + QESD   LTY +C LKE
Sbjct: 315 MFGGTETVASAIEWAMAELMRNPDDLKRVQQELSDVVGLHRKVQESDFEKLTYFKCCLKE 374

Query: 61  TLRLHPPIPVLLHETAAETELARSRIPAKSRVFINAWAIGRDEKSRVDPDIFRPARFEIE 120
           TLRLHPPIP+LLHETA  +E+A  RIPAKSRV INAWAIGRD+ S  DPD FRPARF  E
Sbjct: 375 TLRLHPPIPLLLHETAEASEIAGYRIPAKSRVMINAWAIGRDKDSWKDPDTFRPARFLEE 434

Query: 121 GAADFKGGDFEFIPFGSGRRSCPGM 146
           G  DFKG +FEFIPFGSGRRSCPGM
Sbjct: 435 GVPDFKGSNFEFIPFGSGRRSCPGM 459

[Close]

BLAST of CSPI04G05160 vs. NCBI nr
Match: gi|702467402|ref|XP_010029715.1| (PREDICTED: cytochrome P450 84A1-like [Eucalyptus grandis])

HSP 1 Score: 218.0 bits (554), Expect = 1.1e-53
Identity = 102/145 (70.34%), Postives = 121/145 (83.45%), Query Frame = 1


Query: 1   MFGGTETVASAIEWTMAELMKNPDDLEKLQQQLDDTVGLHHRPQESDLHNLTYLRCILKE 60
           MFGGTETVASAIEW M ELM++P+DL+K+Q++L D VGLH R +ESD   LTYL+C++KE
Sbjct: 320 MFGGTETVASAIEWAMTELMRSPEDLKKVQKELADVVGLHRRVEESDFEKLTYLKCVIKE 379

Query: 61  TLRLHPPIPVLLHETAAETELARSRIPAKSRVFINAWAIGRDEKSRVDPDIFRPARFEIE 120
           TLRLHPPIP+LLHETA + E++   IPAK+RV INAWAIGRD  S  DPD F+P+RF  E
Sbjct: 380 TLRLHPPIPLLLHETAEDAEVSGYFIPAKTRVMINAWAIGRDPTSWEDPDTFKPSRFLNE 439

Query: 121 GAADFKGGDFEFIPFGSGRRSCPGM 146
           GA DFKG +FEFIPFGSGRRSCPGM
Sbjct: 440 GAPDFKGSNFEFIPFGSGRRSCPGM 464

[Close]

BLAST of CSPI04G05160 vs. NCBI nr
Match: gi|225431217|ref|XP_002272644.1| (PREDICTED: cytochrome P450 84A1 [Vitis vinifera])

HSP 1 Score: 217.2 bits (552), Expect = 1.9e-53
Identity = 102/145 (70.34%), Postives = 122/145 (84.14%), Query Frame = 1


Query: 1   MFGGTETVASAIEWTMAELMKNPDDLEKLQQQLDDTVGLHHRPQESDLHNLTYLRCILKE 60
           MFGGTETVASAIEW MAE+MK+P+DL+K+QQ+L D VGL+ R +ESDL  LTYL+C+LKE
Sbjct: 310 MFGGTETVASAIEWAMAEMMKSPEDLKKVQQELADVVGLNRRVEESDLEKLTYLKCVLKE 369

Query: 61  TLRLHPPIPVLLHETAAETELARSRIPAKSRVFINAWAIGRDEKSRVDPDIFRPARFEIE 120
           TLRLHPPIP+LLHETA + E+A   IPA+SRV INAWAIGRD+ S  +P+ F+P+RF   
Sbjct: 370 TLRLHPPIPLLLHETAEDAEVAGYHIPARSRVMINAWAIGRDKNSWDEPETFKPSRFLKA 429

Query: 121 GAADFKGGDFEFIPFGSGRRSCPGM 146
           G  DFKG +FEFIPFGSGRRSCPGM
Sbjct: 430 GVPDFKGSNFEFIPFGSGRRSCPGM 454

[Close]

BLAST of CSPI04G05160 vs. NCBI nr
Match: gi|147787265|emb|CAN73481.1| (hypothetical protein VITISV_026695 [Vitis vinifera])

HSP 1 Score: 215.7 bits (548), Expect = 5.4e-53
Identity = 102/145 (70.34%), Postives = 121/145 (83.45%), Query Frame = 1


Query: 1   MFGGTETVASAIEWTMAELMKNPDDLEKLQQQLDDTVGLHHRPQESDLHNLTYLRCILKE 60
           MFGGTETVASAIEW MAE+MK+P+DL+K+QQ+L D VGL+ R +ESDL  LTYL+C LKE
Sbjct: 289 MFGGTETVASAIEWAMAEMMKSPEDLKKVQQELADVVGLNRRVEESDLEKLTYLKCALKE 348

Query: 61  TLRLHPPIPVLLHETAAETELARSRIPAKSRVFINAWAIGRDEKSRVDPDIFRPARFEIE 120
           TLRLHPPIP+LLHETA + E+A   IPA+SRV INAWAIGRD+ S  +P+ F+P+RF   
Sbjct: 349 TLRLHPPIPLLLHETAEDAEVAGYHIPARSRVMINAWAIGRDKNSWDEPETFKPSRFLKA 408

Query: 121 GAADFKGGDFEFIPFGSGRRSCPGM 146
           G  DFKG +FEFIPFGSGRRSCPGM
Sbjct: 409 GVPDFKGSNFEFIPFGSGRRSCPGM 433

The following BLAST results are available for this feature:

BLAST of CSPI04G05160 vs. Swiss-Prot


Match Name	E-value	Identity	Description
C84A1_ARATH	2.3e-51	66.21	Cytochrome P450 84A1 OS=Arabidopsis thaliana GN=CYP84A1 PE=1 SV=1	[more]
C84A4_ARATH	3.3e-42	55.48	Cytochrome P450 84A4 OS=Arabidopsis thaliana GN=CYP84A4 PE=1 SV=1	[more]
C71A1_PERAE	4.1e-37	53.47	Cytochrome P450 71A1 OS=Persea americana GN=CYP71A1 PE=1 SV=2	[more]
C76B6_CATRO	1.5e-34	47.55	Geraniol 8-hydroxylase OS=Catharanthus roseus GN=CYP76B6 PE=1 SV=1	[more]
C71DI_MENGR	5.6e-34	49.30	Cytochrome P450 71D18 OS=Mentha gracilis GN=CYP71D18 PE=1 SV=1	[more]

BLAST of CSPI04G05160 vs. TrEMBL


Match Name	E-value	Identity	Description
Q6PLI4_CENER	5.6e-57	75.17	Coniferylalcohol 5-hydroxylase OS=Centaurium erythraea PE=2 SV=1	[more]
A0A059ASK6_EUCGR	7.6e-54	70.34	Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_I02371 PE=3 SV=1	[more]
F6GWQ4_VITVI	1.3e-53	70.34	Putative uncharacterized protein OS=Vitis vinifera GN=VIT_04s0023g02900 PE=3 SV=...	[more]
A5AK80_VITVI	3.8e-53	70.34	Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_026695 PE=3 SV=1	[more]
A0A088B182_9ROSI	4.9e-53	69.66	Putative ferulate 5-hydroxylase OS=Hibiscus cannabinus GN=F5H PE=2 SV=1	[more]

BLAST of CSPI04G05160 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G36220.1	1.3e-52	66.21	ferulic acid 5-hydroxylase 1	[more]
AT5G04330.1	1.8e-43	55.48	Cytochrome P450 superfamily protein	[more]
AT3G52970.2	1.5e-32	47.95	cytochrome P450, family 76, subfamily G, polypeptide 1	[more]
AT5G06900.1	3.3e-32	46.48	cytochrome P450, family 93, subfamily D, polypeptide 1	[more]
AT4G22710.1	1.6e-31	44.76	cytochrome P450, family 706, subfamily A, polypeptide 2	[more]

BLAST of CSPI04G05160 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659107516\|ref\|XP_008453715.1\|	1.4e-72	93.10	PREDICTED: LOW QUALITY PROTEIN: cytochrome P450 84A1-like [Cucumis melo]	[more]
gi\|46403211\|gb\|AAS92625.1\|	8.0e-57	75.17	coniferylalcohol 5-hydroxylase [Centaurium erythraea]	[more]
gi\|702467402\|ref\|XP_010029715.1\|	1.1e-53	70.34	PREDICTED: cytochrome P450 84A1-like [Eucalyptus grandis]	[more]
gi\|225431217\|ref\|XP_002272644.1\|	1.9e-53	70.34	PREDICTED: cytochrome P450 84A1 [Vitis vinifera]	[more]
gi\|147787265\|emb\|CAN73481.1\|	5.4e-53	70.34	hypothetical protein VITISV_026695 [Vitis vinifera]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR001128	Cyt_P450
IPR002401	Cyt_P450_E_grp-I
IPR017972	Cyt_P450_CS

Vocabulary: **Molecular Function**
Term	Definition
GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
GO:0005506	iron ion binding
GO:0020037	heme binding

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	oxidation-reduction process

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055114	oxidation-reduction process
cellular_component	GO:0005575	cellular_component
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI04G05160.1	CSPI04G05160.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 133..142 score: 2.3E-12coord: 56..67 score: 2.3E-12coord: 142..145 score: 2.3E-12coord: 3..20 score: 2.3
IPR001128	Cytochrome P450	GENE3D	G3DSA:1.10.630.10		coord: 1..145 score: 1.0
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 1..145 score: 1.3
IPR001128	Cytochrome P450	unknown	SSF48264	Cytochrome P450	coord: 1..145 score: 1.96
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 55..73 score: 2.2E-20coord: 132..142 score: 2.2E-20coord: 95..119 score: 2.2E-20coord: 142..145 score: 2.2E-20coord: 12..38 score: 2.2
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 135..144 scor
None	No IPR available	PANTHER	PTHR24298	FAMILY NOT NAMED	coord: 1..145 score: 6.9
None	No IPR available	PANTHER	PTHR24298:SF60	CYTOCHROME P450 84A1-RELATED	coord: 1..145 score: 6.9

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CSPI04G05160	Csa4G043910	Cucumber (Chinese Long) v2	cpicuB182
CSPI04G05160	Lsi07G002250	Bottle gourd (USVL1VR-Ls)	cpilsiB303
CSPI04G05160	MELO3C017540.2	Melon (DHL92) v3.6.1	cpimedB301
CSPI04G05160	MELO3C007884.2	Melon (DHL92) v3.6.1	cpimedB328
CSPI04G05160	CsaV3_6G046880	Cucumber (Chinese Long) v3	cpicucB242
CSPI04G05160	Cla97C01G019500	Watermelon (97103) v2	cpiwmbB275
CSPI04G05160	Cla97C07G135930	Watermelon (97103) v2	cpiwmbB332
CSPI04G05160	Bhi03G000666	Wax gourd	cpiwgoB407
CSPI04G05160	Cucsa.201190	Cucumber (Gy14) v1	cgycpiB319
CSPI04G05160	Cucsa.043380	Cucumber (Gy14) v1	cgycpiB026
CSPI04G05160	CmaCh07G004450	Cucurbita maxima (Rimu)	cmacpiB881
CSPI04G05160	CmoCh07G004420	Cucurbita moschata (Rifu)	cmocpiB861
CSPI04G05160	CsGy6G031100	Cucumber (Gy14) v2	cgybcpiB279

The following gene(s) are paralogous to this gene:


Gene	Paralogue	Organism	Block
CSPI04G05160	CSPI06G31560	Wild cucumber (PI 183967)	cpicpiB163

The following block(s) are covering this gene:


Gene	Organism	Block
CSPI04G05160	Cucurbita maxima (Rimu)	cmacpiB342
CSPI04G05160	Cucurbita maxima (Rimu)	cmacpiB675
CSPI04G05160	Cucurbita maxima (Rimu)	cmacpiB721
CSPI04G05160	Cucurbita moschata (Rifu)	cmocpiB332
CSPI04G05160	Cucurbita moschata (Rifu)	cmocpiB666
CSPI04G05160	Cucurbita moschata (Rifu)	cmocpiB715
CSPI04G05160	Cucumber (Chinese Long) v2	cpicuB201
CSPI04G05160	Melon (DHL92) v3.5.1	cpimeB267
CSPI04G05160	Melon (DHL92) v3.5.1	cpimeB310
CSPI04G05160	Melon (DHL92) v3.5.1	cpimeB338
CSPI04G05160	Watermelon (Charleston Gray)	cpiwcgB300
CSPI04G05160	Watermelon (Charleston Gray)	cpiwcgB342
CSPI04G05160	Watermelon (97103) v1	cpiwmB313
CSPI04G05160	Watermelon (97103) v1	cpiwmB335
CSPI04G05160	Cucurbita pepo (Zucchini)	cpecpiB223
CSPI04G05160	Cucurbita pepo (Zucchini)	cpecpiB397
CSPI04G05160	Bottle gourd (USVL1VR-Ls)	cpilsiB252
CSPI04G05160	Melon (DHL92) v3.6.1	cpimedB263
CSPI04G05160	Cucumber (Gy14) v2	cgybcpiB168
CSPI04G05160	Silver-seed gourd	carcpiB0444
CSPI04G05160	Silver-seed gourd	carcpiB0981
CSPI04G05160	Silver-seed gourd	carcpiB1057
CSPI04G05160	Cucumber (Chinese Long) v3	cpicucB208
CSPI04G05160	Cucumber (Chinese Long) v3	cpicucB213
CSPI04G05160	Wax gourd	cpiwgoB378