CSPI04G03340 (gene) Wild cucumber (PI 183967)

NameCSPI04G03340
Typegene
OrganismCucumis sativus (Wild cucumber (PI 183967))
DescriptionCysteine protease
LocationChr4 : 2053741 .. 2055047 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACCATGATGAAATTTCTTATTGTTTTCGTTGTTTTGATTGCTTTCACGTCTCATTTGTGTGAGAGCTTTGAGTTGGAAGGAAAGGATTTTGAATCTGAAAGAAGTCTCATGCAACTCTACAAAAGATGGAGTAGCCACCATAGAATCTCAAGAAATGCACATGAGATGCACAAACGTTTCAAGATCTTTCAAGATAATGCAAAACATGTGTTTAGAGTGAACCACATGGGAAAATCATTAAAATTGCGACTTAACCAGTTTGCTGATTTGTCTGATGATGAGTTTAGTATGATGTACGGTTCCAACATTACTCACTACAACAGCTTACATGCCAATCGTGTTGGTGGATTTATGTATGAACGAGCAATGAATATCCCATCTTCGATCGATTGGAGGCAAAGAGGAGCTGTGAATGCCATAAAAAATCAAGGCCGTTGTGGTAATTAATTTAATTTCATTTATAATCTTTCTAAAGCCTAATGCCTTCTAGCTAATAGTTACTAATGCCTTCTAATAATTATGTGTTATATGTTCGACAGGAAGTTGTTGGGCGTTTGCAGCTGTGGCTGCCGTGGAATCTATTCACCAAATAAGAACAAATGAATTAGTATCTCTATCAGAGCAAGAAGTGGTGGATTGTGATTATAAAGTCGGTGGTTGTCGTGGAGGAAATTATGACTCTGCATTTGAGTTCATAATGCAAAATGGTGGAATCACAATTGAGGAAAACTATCCATATTTTGCAGGAAACGGATATTGTCGTAGACGAGGAGTGAGTTTAAACCAATATAATTATGAGTGCATTATTCATTATTGATCATAATATAATTATGTGTATTGTGTTTGATATTTCACTAATAGCCTAACAGTGAGAGAGTAACAATTGATGGATATGAGCGTGTACCTCAAAACAACGAGTATGCTTTGATGAAAGCAGTGGCACACCAACCGGTAGCAGTGTCGGTAGCTTCAAGTGGGAGTGATTTTAGATTTTACGGGGAGGCAAGTGTAGTTAATTTTGTTGTTGTTAATTATAATTTGGTGCAACATATATAATTAAAAGAAAAAATGATAATTATTTGCAGGGAATGCTTAGAGAAGATAGCTTTTGCGGATATAGAATTGACCACACGGTAGTGGTAGTTGGGTATGGAAGTGATGAAGAGGGAGATTATTGGATAATAAGAAACCAATATGGAACTCAATGGGGAATGAATGGTTACATGAAGATGCAACGAGGAACACGAAACCCACAAGGTGTATGTGGAATGGCGATGCAACCTTCCTTTCCCGTCAAGTATTGA

mRNA sequence

ATGACCATGATGAAATTTCTTATTGTTTTCGTTGTTTTGATTGCTTTCACGTCTCATTTGTGTGAGAGCTTTGAGTTGGAAGGAAAGGATTTTGAATCTGAAAGAAGTCTCATGCAACTCTACAAAAGATGGAGTAGCCACCATAGAATCTCAAGAAATGCACATGAGATGCACAAACGTTTCAAGATCTTTCAAGATAATGCAAAACATGTGTTTAGAGTGAACCACATGGGAAAATCATTAAAATTGCGACTTAACCAGTTTGCTGATTTGTCTGATGATGAGTTTAGTATGATGTACGGTTCCAACATTACTCACTACAACAGCTTACATGCCAATCGTGTTGGTGGATTTATGTATGAACGAGCAATGAATATCCCATCTTCGATCGATTGGAGGCAAAGAGGAGCTGTGAATGCCATAAAAAATCAAGGCCGTTGTGGAAGTTGTTGGGCGTTTGCAGCTGTGGCTGCCGTGGAATCTATTCACCAAATAAGAACAAATGAATTAGTATCTCTATCAGAGCAAGAAGTGGTGGATTGTGATTATAAAGTCGGTGGTTGTCGTGGAGGAAATTATGACTCTGCATTTGAGTTCATAATGCAAAATGGTGGAATCACAATTGAGGAAAACTATCCATATTTTGCAGGAAACGGATATTGTCGTAGACGAGGACCTAACAGTGAGAGAGTAACAATTGATGGATATGAGCGTGTACCTCAAAACAACGAGTATGCTTTGATGAAAGCAGTGGCACACCAACCGGTAGCAGTGTCGGTAGCTTCAAGTGGGAGTGATTTTAGATTTTACGGGGAGGCAAGTGGAATGCTTAGAGAAGATAGCTTTTGCGGATATAGAATTGACCACACGGTAGTGGTAGTTGGGTATGGAAGTGATGAAGAGGGAGATTATTGGATAATAAGAAACCAATATGGAACTCAATGGGGAATGAATGGTTACATGAAGATGCAACGAGGAACACGAAACCCACAAGGTGTATGTGGAATGGCGATGCAACCTTCCTTTCCCGTCAAGTATTGA

Coding sequence (CDS)

ATGACCATGATGAAATTTCTTATTGTTTTCGTTGTTTTGATTGCTTTCACGTCTCATTTGTGTGAGAGCTTTGAGTTGGAAGGAAAGGATTTTGAATCTGAAAGAAGTCTCATGCAACTCTACAAAAGATGGAGTAGCCACCATAGAATCTCAAGAAATGCACATGAGATGCACAAACGTTTCAAGATCTTTCAAGATAATGCAAAACATGTGTTTAGAGTGAACCACATGGGAAAATCATTAAAATTGCGACTTAACCAGTTTGCTGATTTGTCTGATGATGAGTTTAGTATGATGTACGGTTCCAACATTACTCACTACAACAGCTTACATGCCAATCGTGTTGGTGGATTTATGTATGAACGAGCAATGAATATCCCATCTTCGATCGATTGGAGGCAAAGAGGAGCTGTGAATGCCATAAAAAATCAAGGCCGTTGTGGAAGTTGTTGGGCGTTTGCAGCTGTGGCTGCCGTGGAATCTATTCACCAAATAAGAACAAATGAATTAGTATCTCTATCAGAGCAAGAAGTGGTGGATTGTGATTATAAAGTCGGTGGTTGTCGTGGAGGAAATTATGACTCTGCATTTGAGTTCATAATGCAAAATGGTGGAATCACAATTGAGGAAAACTATCCATATTTTGCAGGAAACGGATATTGTCGTAGACGAGGACCTAACAGTGAGAGAGTAACAATTGATGGATATGAGCGTGTACCTCAAAACAACGAGTATGCTTTGATGAAAGCAGTGGCACACCAACCGGTAGCAGTGTCGGTAGCTTCAAGTGGGAGTGATTTTAGATTTTACGGGGAGGCAAGTGGAATGCTTAGAGAAGATAGCTTTTGCGGATATAGAATTGACCACACGGTAGTGGTAGTTGGGTATGGAAGTGATGAAGAGGGAGATTATTGGATAATAAGAAACCAATATGGAACTCAATGGGGAATGAATGGTTACATGAAGATGCAACGAGGAACACGAAACCCACAAGGTGTATGTGGAATGGCGATGCAACCTTCCTTTCCCGTCAAGTATTGA
BLAST of CSPI04G03340 vs. Swiss-Prot
Match: CYSEP_RICCO (Vignain OS=Ricinus communis GN=CYSEP PE=1 SV=1)

HSP 1 Score: 343.6 bits (880), Expect = 2.5e-93
Identity = 172/348 (49.43%), Postives = 239/348 (68.68%), Query Frame = 1

Query: 3   MMKFLIVFVVLIAFTSHLCESFELEGKDFESERSLMQLYKRWSSHHRISRNAHEMHKRFK 62
           M KF+++ + L A    + ESF+   K+ ESE SL  LY+RW SHH +SR+ HE  KRF 
Sbjct: 1   MQKFILLALSL-ALVLAITESFDFHEKELESEESLWGLYERWRSHHTVSRSLHEKQKRFN 60

Query: 63  IFQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMY-GSNITHYNSLHANRVGG--FM 122
           +F+ NA HV   N M K  KL+LN+FAD+++ EF   Y GS + H+        G   FM
Sbjct: 61  VFKHNAMHVHNANKMDKPYKLKLNKFADMTNHEFRNTYSGSKVKHHRMFRGGPRGNGTFM 120

Query: 123 YERAMNIPSSIDWRQRGAVNAIKNQGRCGSCWAFAAVAAVESIHQIRTNELVSLSEQEVV 182
           YE+   +P+S+DWR++GAV ++K+QG+CGSCWAF+ + AVE I+QI+TN+LVSLSEQE+V
Sbjct: 121 YEKVDTVPASVDWRKKGAVTSVKDQGQCGSCWAFSTIVAVEGINQIKTNKLVSLSEQELV 180

Query: 183 DCDYKVG-GCRGGNYDSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNSERVTIDGYER 242
           DCD     GC GG  D AFEFI Q GGIT E NYPY A +G C     N+  V+IDG+E 
Sbjct: 181 DCDTDQNQGCNGGLMDYAFEFIKQRGGITTEANYPYEAYDGTCDVSKENAPAVSIDGHEN 240

Query: 243 VPQNNEYALMKAVAHQPVAVSVASSGSDFRFYGEASGMLREDSFCGYRIDHTVVVVGYGS 302
           VP+N+E AL+KAVA+QPV+V++ + GSDF+FY E  G+      CG  +DH V +VGYG+
Sbjct: 241 VPENDENALLKAVANQPVSVAIDAGGSDFQFYSE--GVF--TGSCGTELDHGVAIVGYGT 300

Query: 303 DEEG-DYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVK 346
             +G  YW ++N +G +WG  GY++M+RG  + +G+CG+AM+ S+P+K
Sbjct: 301 TIDGTKYWTVKNSWGPEWGEKGYIRMERGISDKEGLCGIAMEASYPIK 343

BLAST of CSPI04G03340 vs. Swiss-Prot
Match: CYSEP_VIGMU (Vignain OS=Vigna mungo PE=1 SV=1)

HSP 1 Score: 343.6 bits (880), Expect = 2.5e-93
Identity = 171/347 (49.28%), Postives = 236/347 (68.01%), Query Frame = 1

Query: 4   MKFLIVFVVLIAFTSHLCESFELEGKDFESERSLMQLYKRWSSHHRISRNAHEMHKRFKI 63
           MK L+  V+ ++    +  SF+   KD ESE SL  LY+RW SHH +SR+  E HKRF +
Sbjct: 3   MKKLLWVVLSLSLVLGVANSFDFHEKDLESEESLWDLYERWRSHHTVSRSLGEKHKRFNV 62

Query: 64  FQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMY-GSNITHYNSLHANRVGG--FMY 123
           F+ N  HV   N M K  KL+LN+FAD+++ EF   Y GS + H+     ++ G   FMY
Sbjct: 63  FKANVMHVHNTNKMDKPYKLKLNKFADMTNHEFRSTYAGSKVNHHKMFRGSQHGSGTFMY 122

Query: 124 ERAMNIPSSIDWRQRGAVNAIKNQGRCGSCWAFAAVAAVESIHQIRTNELVSLSEQEVVD 183
           E+  ++P+S+DWR++GAV  +K+QG+CGSCWAF+ + AVE I+QI+TN+LVSLSEQE+VD
Sbjct: 123 EKVGSVPASVDWRKKGAVTDVKDQGQCGSCWAFSTIVAVEGINQIKTNKLVSLSEQELVD 182

Query: 184 CDYKVG-GCRGGNYDSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNSERVTIDGYERV 243
           CD +   GC GG  +SAFEFI Q GGIT E NYPY A  G C     N   V+IDG+E V
Sbjct: 183 CDKEENQGCNGGLMESAFEFIKQKGGITTESNYPYTAQEGTCDESKVNDLAVSIDGHENV 242

Query: 244 PQNNEYALMKAVAHQPVAVSVASSGSDFRFYGEASGMLREDSFCGYRIDHTVVVVGYGSD 303
           P N+E AL+KAVA+QPV+V++ + GSDF+FY E  G+   D  C   ++H V +VGYG+ 
Sbjct: 243 PVNDENALLKAVANQPVSVAIDAGGSDFQFYSE--GVFTGD--CNTDLNHGVAIVGYGTT 302

Query: 304 EEG-DYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVK 346
            +G +YWI+RN +G +WG  GY++MQR     +G+CG+AM  S+P+K
Sbjct: 303 VDGTNYWIVRNSWGPEWGEQGYIRMQRNISKKEGLCGIAMMASYPIK 345

BLAST of CSPI04G03340 vs. Swiss-Prot
Match: CYSEP_PHAVU (Vignain OS=Phaseolus vulgaris PE=2 SV=2)

HSP 1 Score: 338.6 bits (867), Expect = 8.2e-92
Identity = 170/346 (49.13%), Postives = 233/346 (67.34%), Query Frame = 1

Query: 5   KFLIVFVVLIAFTSHLCESFELEGKDFESERSLMQLYKRWSSHHRISRNAHEMHKRFKIF 64
           K L+  V+  +    +  SF+   KD  SE SL  LY+RW SHH +SR+  E HKRF +F
Sbjct: 4   KKLLWVVLSFSLVLGVANSFDFHDKDLASEESLWDLYERWRSHHTVSRSLGEKHKRFNVF 63

Query: 65  QDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMY-GSNITHYNSLHA--NRVGGFMYE 124
           + N  HV   N M K  KL+LN+FAD+++ EF   Y GS + H        +  G FMYE
Sbjct: 64  KANLMHVHNTNKMDKPYKLKLNKFADMTNHEFRSTYAGSKVNHPRMFRGTPHENGAFMYE 123

Query: 125 RAMNIPSSIDWRQRGAVNAIKNQGRCGSCWAFAAVAAVESIHQIRTNELVSLSEQEVVDC 184
           + +++P S+DWR++GAV  +K+QG+CGSCWAF+ V AVE I+QI+TN+LV+LSEQE+VDC
Sbjct: 124 KVVSVPPSVDWRKKGAVTDVKDQGQCGSCWAFSTVVAVEGINQIKTNKLVALSEQELVDC 183

Query: 185 DYKVG-GCRGGNYDSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNSERVTIDGYERVP 244
           D +   GC GG  +SAFEFI Q GGIT E NYPY A  G C     N   V+IDG+E VP
Sbjct: 184 DKEENQGCNGGLMESAFEFIKQKGGITTESNYPYKAQEGTCDASKVNDLAVSIDGHENVP 243

Query: 245 QNNEYALMKAVAHQPVAVSVASSGSDFRFYGEASGMLREDSFCGYRIDHTVVVVGYGSDE 304
            N+E AL+KAVA+QPV+V++ + GSDF+FY E  G+   D  C   ++H V +VGYG+  
Sbjct: 244 ANDEDALLKAVANQPVSVAIDAGGSDFQFYSE--GVFTGD--CSTDLNHGVAIVGYGTTV 303

Query: 305 EG-DYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVK 346
           +G +YWI+RN +G +WG +GY++MQR     +G+CG+AM PS+P+K
Sbjct: 304 DGTNYWIVRNSWGPEWGEHGYIRMQRNISKKEGLCGIAMLPSYPIK 345

BLAST of CSPI04G03340 vs. Swiss-Prot
Match: CEP3_ARATH (KDEL-tailed cysteine endopeptidase CEP3 OS=Arabidopsis thaliana GN=CEP3 PE=2 SV=1)

HSP 1 Score: 335.5 bits (859), Expect = 6.9e-91
Identity = 169/346 (48.84%), Postives = 233/346 (67.34%), Query Frame = 1

Query: 8   IVFVVLIAFTSHLCES--FELEGKDFESERSLMQLYKRWSSHHRISRNAHEMHKRFKIFQ 67
           + F+VLI+F S L  S  F+ + K+ E+E ++ +LY+RW  HH +SR +HE  KRF +F+
Sbjct: 3   LFFIVLISFLSLLQASKGFDFDEKELETEENVWKLYERWRGHHSVSRASHEAIKRFNVFR 62

Query: 68  DNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMY-GSNITHYNSLHANR--VGGFMYER 127
            N  HV R N   K  KL++N+FAD++  EF   Y GSN+ H+  L   +   GGFMYE 
Sbjct: 63  HNVLHVHRTNKKNKPYKLKINRFADITHHEFRSSYAGSNVKHHRMLRGPKRGSGGFMYEN 122

Query: 128 AMNIPSSIDWRQRGAVNAIKNQGRCGSCWAFAAVAAVESIHQIRTNELVSLSEQEVVDCD 187
              +PSS+DWR++GAV  +KNQ  CGSCWAF+ VAAVE I++IRTN+LVSLSEQE+VDCD
Sbjct: 123 VTRVPSSVDWREKGAVTEVKNQQDCGSCWAFSTVAAVEGINKIRTNKLVSLSEQELVDCD 182

Query: 188 YKVG-GCRGGNYDSAFEFIMQNGGITIEENYPYFAGN-GYCRRRGPNSERVTIDGYERVP 247
            +   GC GG  + AFEFI  NGGI  EE YPY + +  +CR      E VTIDG+E VP
Sbjct: 183 TEENQGCAGGLMEPAFEFIKNNGGIKTEETYPYDSSDVQFCRANSIGGETVTIDGHEHVP 242

Query: 248 QNNEYALMKAVAHQPVAVSVASSGSDFRFYGEASGMLREDSFCGYRIDHTVVVVGYGSDE 307
           +N+E  L+KAVAHQPV+V++ +  SDF+ Y E   +      CG +++H VV+VGYG  +
Sbjct: 243 ENDEEELLKAVAHQPVSVAIDAGSSDFQLYSEGVFI----GECGTQLNHGVVIVGYGETK 302

Query: 308 EG-DYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVK 346
            G  YWI+RN +G +WG  GY++++RG    +G CG+AM+ S+P K
Sbjct: 303 NGTKYWIVRNSWGPEWGEGGYVRIERGISENEGRCGIAMEASYPTK 344

BLAST of CSPI04G03340 vs. Swiss-Prot
Match: CEP2_ARATH (KDEL-tailed cysteine endopeptidase CEP2 OS=Arabidopsis thaliana GN=CEP2 PE=1 SV=1)

HSP 1 Score: 334.7 bits (857), Expect = 1.2e-90
Identity = 172/349 (49.28%), Postives = 232/349 (66.48%), Query Frame = 1

Query: 3   MMKFLIVFVVLIAFTSHLCESFELEGKDFESERSLMQLYKRWSSHHRISRNAHEMHKRFK 62
           M K L++F+  +      C  F+ + K+ ESE  L  LY RW SHH + R+ +E  KRF 
Sbjct: 1   MKKLLLIFLFSLVILQTAC-GFDYDDKEIESEEGLSTLYDRWRSHHSVPRSLNEREKRFN 60

Query: 63  IFQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMY-GSNITHYNSLHANRVGG--FM 122
           +F+ N  HV   N   +S KL+LN+FADL+ +EF   Y GSNI H+  L   + G   FM
Sbjct: 61  VFRHNVMHVHNTNKKNRSYKLKLNKFADLTINEFKNAYTGSNIKHHRMLQGPKRGSKQFM 120

Query: 123 Y--ERAMNIPSSIDWRQRGAVNAIKNQGRCGSCWAFAAVAAVESIHQIRTNELVSLSEQE 182
           Y  E    +PSS+DWR++GAV  IKNQG+CGSCWAF+ VAAVE I++I+TN+LVSLSEQE
Sbjct: 121 YDHENLSKLPSSVDWRKKGAVTEIKNQGKCGSCWAFSTVAAVEGINKIKTNKLVSLSEQE 180

Query: 183 VVDCDYKVG-GCRGGNYDSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNSERVTIDGY 242
           +VDCD K   GC GG  + AFEFI +NGGIT E++YPY   +G C     N   VTIDG+
Sbjct: 181 LVDCDTKQNEGCNGGLMEIAFEFIKKNGGITTEDSYPYEGIDGKCDASKDNGVLVTIDGH 240

Query: 243 ERVPQNNEYALMKAVAHQPVAVSVASSGSDFRFYGEASGMLREDSFCGYRIDHTVVVVGY 302
           E VP+N+E AL+KAVA+QPV+V++ +  SDF+FY E  G+      CG  ++H V  VGY
Sbjct: 241 EDVPENDENALLKAVANQPVSVAIDAGSSDFQFYSE--GVF--TGSCGTELNHGVAAVGY 300

Query: 303 GSDEEGDYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVK 346
           GS+    YWI+RN +G +WG  GY+K++R    P+G CG+AM+ S+P+K
Sbjct: 301 GSERGKKYWIVRNSWGAEWGEGGYIKIEREIDEPEGRCGIAMEASYPIK 344

BLAST of CSPI04G03340 vs. TrEMBL
Match: A0A0A0KGB1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G490180 PE=3 SV=1)

HSP 1 Score: 573.2 bits (1476), Expect = 2.2e-160
Identity = 272/353 (77.05%), Postives = 313/353 (88.67%), Query Frame = 1

Query: 1   MTMMKFLIVFVVLIAFTSHLCESFELEGKDFESERSLMQLYKRWSSHHRISRNAHEMHKR 60
           MT+MKFLIV +VL+AF+ ++CESFELE KDFESE+SLMQLYKRWSSHHRISRNA+EMH R
Sbjct: 1   MTVMKFLIVPLVLVAFSCNICESFELERKDFESEKSLMQLYKRWSSHHRISRNANEMHNR 60

Query: 61  FKIFQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMYGSNITHYNSLHAN------- 120
           FK+F++NAKHVF+VN MGKSLKL+LNQFAD+SDDEF  MY SNIT+Y  LHA        
Sbjct: 61  FKVFKNNAKHVFKVNLMGKSLKLKLNQFADMSDDEFRNMYSSNITYYKDLHAKKIEATGG 120

Query: 121 RVGGFMYERAMNIPSSIDWRQRGAVNAIKNQGRCGSCWAFAAVAAVESIHQIRTNELVSL 180
           R+GGFMYE A NIPSSIDWR++GAVNAIKNQGRCGSCWAFAAVAAVESIHQI+TNELVSL
Sbjct: 121 RIGGFMYEHANNIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELVSL 180

Query: 181 SEQEVVDCDYKVGGCRGGNYDSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNSERVTI 240
           SE+EV+DCDY+ GGCRGG Y+SAFEF+M N G+TIE+NYPY+ GNGYCRRRG  ++RV I
Sbjct: 181 SEEEVLDCDYRDGGCRGGFYNSAFEFMMDNDGVTIEDNYPYYEGNGYCRRRGGRNKRVRI 240

Query: 241 DGYERVPQNNEYALMKAVAHQPVAVSVASSGSDFRFYGEASGMLREDSFCGYRIDHTVVV 300
           DGYE VP+NNEYALMKAVAHQPVAV++AS GSDF+FYG   GM  E+ FCG+ IDHTVVV
Sbjct: 241 DGYENVPRNNEYALMKAVAHQPVAVAIASGGSDFKFYG--GGMFTENDFCGFNIDHTVVV 300

Query: 301 VGYGSDEEGDYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVKY 347
           VGYG+DE+GDYWIIRNQYG +WGMNGYMKMQRG  +PQGVCGMAMQP++PVKY
Sbjct: 301 VGYGTDEDGDYWIIRNQYGHRWGMNGYMKMQRGAHSPQGVCGMAMQPAYPVKY 351

BLAST of CSPI04G03340 vs. TrEMBL
Match: A0A0A0LY73_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G528520 PE=3 SV=1)

HSP 1 Score: 558.5 bits (1438), Expect = 5.6e-156
Identity = 275/347 (79.25%), Postives = 298/347 (85.88%), Query Frame = 1

Query: 3   MMKFLIVFVVLIAFTSHLCESFELEGKDFESERSLMQLYKRWSSHHRISRNAHEMHKRFK 62
           MMKFLIVF+VLIAF SH+CESFELE KDFESE+SLMQLYKRWSSHHRISRN HEM +RFK
Sbjct: 1   MMKFLIVFLVLIAFISHICESFELERKDFESEKSLMQLYKRWSSHHRISRNEHEMDRRFK 60

Query: 63  IFQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMYGSNITHYNSLHAN---RVGGFM 122
           +F+DNAKHVF+VNHMGKSLKL+LNQFAD+SDDEFS  YGSNIT+Y +LHA    RVGGFM
Sbjct: 61  VFKDNAKHVFKVNHMGKSLKLKLNQFADMSDDEFSKTYGSNITYYKNLHAKVGGRVGGFM 120

Query: 123 YERAMNIPSSIDWRQRGAVNAIKNQGRCGSCWAFAAVAAVESIHQIRTNELVSLSEQEVV 182
           YERA NIPSSIDWR++GA        R   CWAFAAVAAVESIHQIRTNELVSLSEQEVV
Sbjct: 121 YERATNIPSSIDWRKKGA--------RRMCCWAFAAVAAVESIHQIRTNELVSLSEQEVV 180

Query: 183 DCDYKVGGCRGGNYDSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNSERVTIDGYERV 242
           DCDYKVGGCRGG+Y SAFEFIM+NGGIT+E NYPY+AG+GYCRRRGPN+ERVTIDGYE V
Sbjct: 181 DCDYKVGGCRGGDYISAFEFIMENGGITVENNYPYYAGDGYCRRRGPNNERVTIDGYENV 240

Query: 243 PQNNEYALMKAVAHQPVAVSVASSGSDFRFYGEASGMLREDSFCGYRIDHTVVVVGYGSD 302
           P+NNEYALMKAVAHQP                   GM  E++FCG RIDHTVVVVGYGSD
Sbjct: 241 PRNNEYALMKAVAHQP-------------------GMFTEENFCGIRIDHTVVVVGYGSD 300

Query: 303 EEGDYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVKY 347
           EEGDYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAM P+FPVKY
Sbjct: 301 EEGDYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMYPAFPVKY 320

BLAST of CSPI04G03340 vs. TrEMBL
Match: A0A0A0KW34_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G013580 PE=3 SV=1)

HSP 1 Score: 441.8 bits (1135), Expect = 7.6e-121
Identity = 215/225 (95.56%), Postives = 220/225 (97.78%), Query Frame = 1

Query: 1   MTMMKFLIVFVVLIAFTSHLCESFELEGKDFESERSLMQLYKRWSSHHRISRNAHEMHKR 60
           MTMMKFLIV VVLIAFT+HLCESFELE KDFESE+SLMQLYKRWSSHHRISRNAHEMHKR
Sbjct: 1   MTMMKFLIVLVVLIAFTTHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNAHEMHKR 60

Query: 61  FKIFQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMYGSNITHYNSLHANRVGGFMY 120
           FKIFQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMYGSNITHYN LHANRVG FMY
Sbjct: 61  FKIFQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMYGSNITHYNGLHANRVGEFMY 120

Query: 121 ERAMNIPSSIDWRQRGAVNAIKNQGRCGSCWAFAAVAAVESIHQIRTNELVSLSEQEVVD 180
           ERAMNIPSSIDWRQ+GAVNAIKNQG CGSCWAFAAVAAVESIHQI+TNELVSLSEQEVVD
Sbjct: 121 ERAMNIPSSIDWRQKGAVNAIKNQGHCGSCWAFAAVAAVESIHQIKTNELVSLSEQEVVD 180

Query: 181 CDYKVGGCRGGNYDSAFEFIMQNGGITIEENYPYFAGNGYCRRRG 226
           CDYKVGGCRGGNY+SAFEFIMQNGGITIEENYPYFAGNGYCRRRG
Sbjct: 181 CDYKVGGCRGGNYNSAFEFIMQNGGITIEENYPYFAGNGYCRRRG 225

BLAST of CSPI04G03340 vs. TrEMBL
Match: A0A0A0LMU4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G349680 PE=3 SV=1)

HSP 1 Score: 386.3 bits (991), Expect = 3.8e-104
Identity = 188/350 (53.71%), Postives = 256/350 (73.14%), Query Frame = 1

Query: 1   MTMMKFLIVFVVLIAFTSHLCESFELEGKDFESERSLMQLYKRWSSHHRISRNAHEMHKR 60
           M + KFL+V ++LI   S L ESFE + K+  +E SL QLY+RW  HH ISRN  E HKR
Sbjct: 1   MAIGKFLLVPLLLIVLVSGLAESFEFDEKELATEESLWQLYERWGKHHTISRNLKEKHKR 60

Query: 61  FKIFQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMYG-SNITHYNSLHANR--VGG 120
           F +F++N  HVF VN M K  KL+LN+FAD+S+ EF   Y  SNI+HY  LH  R   GG
Sbjct: 61  FSVFKENVNHVFTVNQMDKPYKLKLNKFADMSNYEFVNFYARSNISHYRKLHERRRGAGG 120

Query: 121 FMYERAMNIPSSIDWRQRGAVNAIKNQGRCGSCWAFAAVAAVESIHQIRTNELVSLSEQE 180
           FMYE+  ++PSS+DWR+RGAVNA+K QGRCGSCWAF++VAAVE I++I+TN+L+SLSEQE
Sbjct: 121 FMYEQDTDLPSSVDWRERGAVNAVKEQGRCGSCWAFSSVAAVEGINKIKTNQLLSLSEQE 180

Query: 181 VVDCDYKVGGCRGGNYDSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNSERVTIDGYE 240
           ++DC+Y+  GC GG  + AF+FI +NGGI  E +YPY    G CR    +S  V IDGYE
Sbjct: 181 LLDCNYRNKGCNGGFMEIAFDFIKRNGGIATENSYPYHGSRGLCRSSRISSPIVKIDGYE 240

Query: 241 RVPQNNEYALMKAVAHQPVAVSVASSGSDFRFYGEASGMLREDSFCGYRIDHTVVVVGYG 300
            VP+ NE ALM+AVA+QPV+V++ ++G DF+FY +  G+   D +CG  ++H VV +GYG
Sbjct: 241 SVPE-NEDALMQAVANQPVSVAIDAAGRDFQFYSQ--GVF--DGYCGTELNHGVVAIGYG 300

Query: 301 SDEEG-DYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVKY 347
           + E+G DYW++RN +G  WG +GY++M+RG    +G+CG+AM+ S+P+KY
Sbjct: 301 TTEDGTDYWLVRNSWGVGWGEDGYVRMKRGVEQAEGLCGIAMEASYPIKY 345

BLAST of CSPI04G03340 vs. TrEMBL
Match: A0A0A0KXE7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G015180 PE=3 SV=1)

HSP 1 Score: 380.9 bits (977), Expect = 1.6e-102
Identity = 188/193 (97.41%), Postives = 189/193 (97.93%), Query Frame = 1

Query: 1   MTMMKFLIVFVVLIAFTSHLCESFELEGKDFESERSLMQLYKRWSSHHRISRNAHEMHKR 60
           MTMMKFLIVFVVLIAFTSHLCESFELEGKDFESERSLMQLYKRWSSHHRISRNAHEMHKR
Sbjct: 1   MTMMKFLIVFVVLIAFTSHLCESFELEGKDFESERSLMQLYKRWSSHHRISRNAHEMHKR 60

Query: 61  FKIFQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMYGSNITHYNSLHANRVGGFMY 120
           FKIFQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMYGSNITHYN LHANRVG FMY
Sbjct: 61  FKIFQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMYGSNITHYNGLHANRVGEFMY 120

Query: 121 ERAMNIPSSIDWRQRGAVNAIKNQGRCGSCWAFAAVAAVESIHQIRTNELVSLSEQEVVD 180
           ERAMNIPSSIDWRQ+GAVNAIKNQG CGSCWAFAAVAAVESIHQIRTNELVSLSEQ VVD
Sbjct: 121 ERAMNIPSSIDWRQKGAVNAIKNQGHCGSCWAFAAVAAVESIHQIRTNELVSLSEQAVVD 180

Query: 181 CDYKVGGCRGGNY 194
           CDYKVGGCRGGNY
Sbjct: 181 CDYKVGGCRGGNY 193

BLAST of CSPI04G03340 vs. TAIR10
Match: AT3G48350.1 (AT3G48350.1 Cysteine proteinases superfamily protein)

HSP 1 Score: 335.5 bits (859), Expect = 3.9e-92
Identity = 169/346 (48.84%), Postives = 233/346 (67.34%), Query Frame = 1

Query: 8   IVFVVLIAFTSHLCES--FELEGKDFESERSLMQLYKRWSSHHRISRNAHEMHKRFKIFQ 67
           + F+VLI+F S L  S  F+ + K+ E+E ++ +LY+RW  HH +SR +HE  KRF +F+
Sbjct: 3   LFFIVLISFLSLLQASKGFDFDEKELETEENVWKLYERWRGHHSVSRASHEAIKRFNVFR 62

Query: 68  DNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMY-GSNITHYNSLHANR--VGGFMYER 127
            N  HV R N   K  KL++N+FAD++  EF   Y GSN+ H+  L   +   GGFMYE 
Sbjct: 63  HNVLHVHRTNKKNKPYKLKINRFADITHHEFRSSYAGSNVKHHRMLRGPKRGSGGFMYEN 122

Query: 128 AMNIPSSIDWRQRGAVNAIKNQGRCGSCWAFAAVAAVESIHQIRTNELVSLSEQEVVDCD 187
              +PSS+DWR++GAV  +KNQ  CGSCWAF+ VAAVE I++IRTN+LVSLSEQE+VDCD
Sbjct: 123 VTRVPSSVDWREKGAVTEVKNQQDCGSCWAFSTVAAVEGINKIRTNKLVSLSEQELVDCD 182

Query: 188 YKVG-GCRGGNYDSAFEFIMQNGGITIEENYPYFAGN-GYCRRRGPNSERVTIDGYERVP 247
            +   GC GG  + AFEFI  NGGI  EE YPY + +  +CR      E VTIDG+E VP
Sbjct: 183 TEENQGCAGGLMEPAFEFIKNNGGIKTEETYPYDSSDVQFCRANSIGGETVTIDGHEHVP 242

Query: 248 QNNEYALMKAVAHQPVAVSVASSGSDFRFYGEASGMLREDSFCGYRIDHTVVVVGYGSDE 307
           +N+E  L+KAVAHQPV+V++ +  SDF+ Y E   +      CG +++H VV+VGYG  +
Sbjct: 243 ENDEEELLKAVAHQPVSVAIDAGSSDFQLYSEGVFI----GECGTQLNHGVVIVGYGETK 302

Query: 308 EG-DYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVK 346
            G  YWI+RN +G +WG  GY++++RG    +G CG+AM+ S+P K
Sbjct: 303 NGTKYWIVRNSWGPEWGEGGYVRIERGISENEGRCGIAMEASYPTK 344

BLAST of CSPI04G03340 vs. TAIR10
Match: AT3G48340.1 (AT3G48340.1 Cysteine proteinases superfamily protein)

HSP 1 Score: 334.7 bits (857), Expect = 6.7e-92
Identity = 172/349 (49.28%), Postives = 232/349 (66.48%), Query Frame = 1

Query: 3   MMKFLIVFVVLIAFTSHLCESFELEGKDFESERSLMQLYKRWSSHHRISRNAHEMHKRFK 62
           M K L++F+  +      C  F+ + K+ ESE  L  LY RW SHH + R+ +E  KRF 
Sbjct: 1   MKKLLLIFLFSLVILQTAC-GFDYDDKEIESEEGLSTLYDRWRSHHSVPRSLNEREKRFN 60

Query: 63  IFQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMY-GSNITHYNSLHANRVGG--FM 122
           +F+ N  HV   N   +S KL+LN+FADL+ +EF   Y GSNI H+  L   + G   FM
Sbjct: 61  VFRHNVMHVHNTNKKNRSYKLKLNKFADLTINEFKNAYTGSNIKHHRMLQGPKRGSKQFM 120

Query: 123 Y--ERAMNIPSSIDWRQRGAVNAIKNQGRCGSCWAFAAVAAVESIHQIRTNELVSLSEQE 182
           Y  E    +PSS+DWR++GAV  IKNQG+CGSCWAF+ VAAVE I++I+TN+LVSLSEQE
Sbjct: 121 YDHENLSKLPSSVDWRKKGAVTEIKNQGKCGSCWAFSTVAAVEGINKIKTNKLVSLSEQE 180

Query: 183 VVDCDYKVG-GCRGGNYDSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNSERVTIDGY 242
           +VDCD K   GC GG  + AFEFI +NGGIT E++YPY   +G C     N   VTIDG+
Sbjct: 181 LVDCDTKQNEGCNGGLMEIAFEFIKKNGGITTEDSYPYEGIDGKCDASKDNGVLVTIDGH 240

Query: 243 ERVPQNNEYALMKAVAHQPVAVSVASSGSDFRFYGEASGMLREDSFCGYRIDHTVVVVGY 302
           E VP+N+E AL+KAVA+QPV+V++ +  SDF+FY E  G+      CG  ++H V  VGY
Sbjct: 241 EDVPENDENALLKAVANQPVSVAIDAGSSDFQFYSE--GVF--TGSCGTELNHGVAAVGY 300

Query: 303 GSDEEGDYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVK 346
           GS+    YWI+RN +G +WG  GY+K++R    P+G CG+AM+ S+P+K
Sbjct: 301 GSERGKKYWIVRNSWGAEWGEGGYIKIEREIDEPEGRCGIAMEASYPIK 344

BLAST of CSPI04G03340 vs. TAIR10
Match: AT5G50260.1 (AT5G50260.1 Cysteine proteinases superfamily protein)

HSP 1 Score: 327.8 bits (839), Expect = 8.1e-90
Identity = 165/347 (47.55%), Postives = 225/347 (64.84%), Query Frame = 1

Query: 4   MKFLIVFVVLIAFTSHLCESFELEGKDFESERSLMQLYKRWSSHHRISRNAHEMHKRFKI 63
           MK  IV  + +       +  +   KD ESE SL +LY+RW SHH ++R+  E  KRF +
Sbjct: 1   MKRFIVLALCMLMVLETTKGLDFHNKDVESENSLWELYERWRSHHTVARSLEEKAKRFNV 60

Query: 64  FQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMY-GSNITHYNSLHANRVG--GFMY 123
           F+ N KH+   N   KS KL+LN+F D++ +EF   Y GSNI H+      +     FMY
Sbjct: 61  FKHNVKHIHETNKKDKSYKLKLNKFGDMTSEEFRRTYAGSNIKHHRMFQGEKKATKSFMY 120

Query: 124 ERAMNIPSSIDWRQRGAVNAIKNQGRCGSCWAFAAVAAVESIHQIRTNELVSLSEQEVVD 183
                +P+S+DWR+ GAV  +KNQG+CGSCWAF+ V AVE I+QIRT +L SLSEQE+VD
Sbjct: 121 ANVNTLPTSVDWRKNGAVTPVKNQGQCGSCWAFSTVVAVEGINQIRTKKLTSLSEQELVD 180

Query: 184 CDYKVG-GCRGGNYDSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNSERVTIDGYERV 243
           CD     GC GG  D AFEFI + GG+T E  YPY A +  C     N+  V+IDG+E V
Sbjct: 181 CDTNQNQGCNGGLMDLAFEFIKEKGGLTSELVYPYKASDETCDTNKENAPVVSIDGHEDV 240

Query: 244 PQNNEYALMKAVAHQPVAVSVASSGSDFRFYGEASGMLREDSFCGYRIDHTVVVVGYGSD 303
           P+N+E  LMKAVA+QPV+V++ + GSDF+FY E     R    CG  ++H V VVGYG+ 
Sbjct: 241 PKNSEDDLMKAVANQPVSVAIDAGGSDFQFYSEGVFTGR----CGTELNHGVAVVGYGTT 300

Query: 304 EEG-DYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVK 346
            +G  YWI++N +G +WG  GY++MQRG R+ +G+CG+AM+ S+P+K
Sbjct: 301 IDGTKYWIVKNSWGEEWGEKGYIRMQRGIRHKEGLCGIAMEASYPLK 343

BLAST of CSPI04G03340 vs. TAIR10
Match: AT1G20850.1 (AT1G20850.1 xylem cysteine peptidase 2)

HSP 1 Score: 284.3 bits (726), Expect = 1.0e-76
Identity = 143/320 (44.69%), Postives = 202/320 (63.12%), Query Frame = 1

Query: 29  KDFESERSLMQLYKRW-SSHHRISRNAHEMHKRFKIFQDNAKHVFRVNHMGKSLKLRLNQ 88
           +D ES   L++L++ W S+  +      E   RF++F+DN KH+   N  GKS  L LN+
Sbjct: 39  EDLESHDKLIELFENWISNFEKAYETVEEKFLRFEVFKDNLKHIDETNKKGKSYWLGLNE 98

Query: 89  FADLSDDEFSMMY-GSNITHYNSLHANRVGGFMYERAMNIPSSIDWRQRGAVNAIKNQGR 148
           FADLS +EF  MY G                F Y     +P S+DWR++GAV  +KNQG 
Sbjct: 99  FADLSHEEFKKMYLGLKTDIVRRDEERSYAEFAYRDVEAVPKSVDWRKKGAVAEVKNQGS 158

Query: 149 CGSCWAFAAVAAVESIHQIRTNELVSLSEQEVVDCDYKVG-GCRGGNYDSAFEFIMQNGG 208
           CGSCWAF+ VAAVE I++I T  L +LSEQE++DCD     GC GG  D AFE+I++NGG
Sbjct: 159 CGSCWAFSTVAAVEGINKIVTGNLTTLSEQELIDCDTTYNNGCNGGLMDYAFEYIVKNGG 218

Query: 209 ITIEENYPYFAGNGYCRRRGPNSERVTIDGYERVPQNNEYALMKAVAHQPVAVSVASSGS 268
           +  EE+YPY    G C  +   SE VTI+G++ VP N+E +L+KA+AHQP++V++ +SG 
Sbjct: 219 LRKEEDYPYSMEEGTCEMQKDESETVTINGHQDVPTNDEKSLLKALAHQPLSVAIDASGR 278

Query: 269 DFRFYGEASGMLREDSFCGYRIDHTVVVVGYGSDEEGDYWIIRNQYGTQWGMNGYMKMQR 328
           +F+FY  + G+   D  CG  +DH V  VGYGS +  DY I++N +G +WG  GY++++R
Sbjct: 279 EFQFY--SGGVF--DGRCGVDLDHGVAAVGYGSSKGSDYIIVKNSWGPKWGEKGYIRLKR 338

Query: 329 GTRNPQGVCGMAMQPSFPVK 346
            T  P+G+CG+    SFP K
Sbjct: 339 NTGKPEGLCGINKMASFPTK 354

BLAST of CSPI04G03340 vs. TAIR10
Match: AT5G45890.1 (AT5G45890.1 senescence-associated gene 12)

HSP 1 Score: 276.2 bits (705), Expect = 2.8e-74
Identity = 144/348 (41.38%), Postives = 219/348 (62.93%), Query Frame = 1

Query: 4   MKFLIVFVVLIAFTSHLCESFELEGKDFESERSLMQLYKRWSSHH-RISRNAHEMHKRFK 63
           +K + +F+ +  F+S  C S  L  +  ++E  + + +  W + H R+  +  E + R+ 
Sbjct: 3   LKHMQIFLFVAIFSS-FCFSITLS-RPLDNELIMQKRHIEWMTKHGRVYADVKEENNRYV 62

Query: 64  IFQDNAKHVFRVNHM--GKSLKLRLNQFADLSDDEFSMMYGS--NITHYNSLHANRVGGF 123
           +F++N + +  +N +  G++ KL +NQFADL++DEF  MY     ++  +S    ++  F
Sbjct: 63  VFKNNVERIEHLNSIPAGRTFKLAVNQFADLTNDEFRSMYTGFKGVSALSSQSQTKMSPF 122

Query: 124 MYERAMN--IPSSIDWRQRGAVNAIKNQGRCGSCWAFAAVAAVESIHQIRTNELVSLSEQ 183
            Y+   +  +P S+DWR++GAV  IKNQG CG CWAF+AVAA+E   QI+  +L+SLSEQ
Sbjct: 123 RYQNVSSGALPVSVDWRKKGAVTPIKNQGSCGCCWAFSAVAAIEGATQIKKGKLISLSEQ 182

Query: 184 EVVDCDYKVGGCRGGNYDSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNSERVTIDGY 243
           ++VDCD    GC GG  D+AFE I   GG+T E NYPY   +  C  +  N +  +I GY
Sbjct: 183 QLVDCDTNDFGCEGGLMDTAFEHIKATGGLTTESNYPYKGEDATCNSKKTNPKATSITGY 242

Query: 244 ERVPQNNEYALMKAVAHQPVAVSVASSGSDFRFYGEASGMLREDSFCGYRIDHTVVVVGY 303
           E VP N+E ALMKAVAHQPV+V +   G DF+FY  +SG+   +  C   +DH V  +GY
Sbjct: 243 EDVPVNDEQALMKAVAHQPVSVGIEGGGFDFQFY--SSGVFTGE--CTTYLDHAVTAIGY 302

Query: 304 GSDEEGD-YWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFP 344
           G    G  YWII+N +GT+WG +GYM++Q+  ++ QG+CG+AM+ S+P
Sbjct: 303 GESTNGSKYWIIKNSWGTKWGESGYMRIQKDVKDKQGLCGLAMKASYP 344

BLAST of CSPI04G03340 vs. NCBI nr
Match: gi|659108975|ref|XP_008454483.1| (PREDICTED: ervatamin-B-like [Cucumis melo])

HSP 1 Score: 592.8 bits (1527), Expect = 3.9e-166
Identity = 285/348 (81.90%), Postives = 319/348 (91.67%), Query Frame = 1

Query: 2   TMMKFLIVFVVLIAFTSHLCESFELEGKDFESERSLMQLYKRWSSHHRISRNAHEMHKRF 61
           T+MKFLIV +VLIA TSHLCESFELE KDFESE+SLMQLYKRWSSHHRISRNA+EMHKRF
Sbjct: 3   TVMKFLIVPLVLIALTSHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNANEMHKRF 62

Query: 62  KIFQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMYGSNITHYNSLHAN--RVGGFM 121
           K+F+DNAKHVF+ NHMG+SLKL+LNQFAD+SDDEFS ++GSNIT+Y +LHA    VGGFM
Sbjct: 63  KVFKDNAKHVFKKNHMGRSLKLQLNQFADMSDDEFSSIHGSNITYYKNLHAKTGHVGGFM 122

Query: 122 YERAMNIPSSIDWRQRGAVNAIKNQGRCGSCWAFAAVAAVESIHQIRTNELVSLSEQEVV 181
           YE A  IPSSIDWR++GAVNAIKNQG CGSCWAFAAVAAVESIHQI+TNELVSLSEQEVV
Sbjct: 123 YEHAKEIPSSIDWRKKGAVNAIKNQGGCGSCWAFAAVAAVESIHQIKTNELVSLSEQEVV 182

Query: 182 DCDYKVGGCRGGNYDSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNSERVTIDGYERV 241
           DCDY+ GGCRGG+Y+SAFEF+M+NGGIT+E+NYPY+ G+GYCRRRG  +ERV IDGYE V
Sbjct: 183 DCDYRDGGCRGGHYNSAFEFMMENGGITVEDNYPYYEGDGYCRRRGGYNERVKIDGYENV 242

Query: 242 PQNNEYALMKAVAHQPVAVSVASSGSDFRFYGEASGMLREDSFCGYRIDHTVVVVGYGSD 301
           P+NNE+ALMKAVAHQPVAV++ASSGSDFRFYG+  GM  E  FCGY IDHTVVVVGYGSD
Sbjct: 243 PRNNEHALMKAVAHQPVAVAIASSGSDFRFYGQ--GMFTEQDFCGYNIDHTVVVVGYGSD 302

Query: 302 EE-GDYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVKY 347
           EE GDYWIIRNQYGTQWGMNGYMKMQRG RNPQGVCGMAMQP++PVKY
Sbjct: 303 EEDGDYWIIRNQYGTQWGMNGYMKMQRGARNPQGVCGMAMQPAYPVKY 348

BLAST of CSPI04G03340 vs. NCBI nr
Match: gi|659108973|ref|XP_008454482.1| (PREDICTED: ervatamin-B-like [Cucumis melo])

HSP 1 Score: 585.9 bits (1509), Expect = 4.7e-164
Identity = 282/349 (80.80%), Postives = 320/349 (91.69%), Query Frame = 1

Query: 1   MTMMKFLIVFVVLIAFTSHLCESFELEGKDFESERSLMQLYKRWSSHHRISRNAHEMHKR 60
           M +MKFLIV +VLIAFT HLCESFELE KDFESE+SLMQLYKRWSSHHRISRNA+EMHKR
Sbjct: 1   MAVMKFLIVPLVLIAFTFHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNANEMHKR 60

Query: 61  FKIFQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMYGSNITHYNSLHAN--RVGGF 120
           FK+F+DNAK+VF+ NHMG+SLKL+LNQFAD+SDDEFS ++GSNIT+Y +LHA   RVGGF
Sbjct: 61  FKVFKDNAKYVFKKNHMGRSLKLQLNQFADMSDDEFSSIHGSNITYYKNLHAKNGRVGGF 120

Query: 121 MYERAMNIPSSIDWRQRGAVNAIKNQGRCGSCWAFAAVAAVESIHQIRTNELVSLSEQEV 180
           MYE A +IPSSIDWR++GAVNAIKNQGRCGSCWAFAAVAAVESIHQI+TNELVSLSEQEV
Sbjct: 121 MYEHANDIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELVSLSEQEV 180

Query: 181 VDCDYKVGGCRGGNYDSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNSERVTIDGYER 240
           VDCDY+  GC GG Y+SAFEF+M+NGGIT+E+NYPY+ G+GYCRRRG  +ERVTIDGYE 
Sbjct: 181 VDCDYRDSGCLGGFYNSAFEFMMENGGITVEDNYPYYEGDGYCRRRGGYNERVTIDGYEN 240

Query: 241 VPQNNEYALMKAVAHQPVAVSVASSGSDFRFYGEASGMLREDSFCGYRIDHTVVVVGYGS 300
           VP+NNE+ALMKAVAHQPVAV++ASSGSDFRFYG+  GM  E  FCGY IDHTVVVVGYG+
Sbjct: 241 VPRNNEHALMKAVAHQPVAVAIASSGSDFRFYGQ--GMFTEQDFCGYNIDHTVVVVGYGT 300

Query: 301 DEE-GDYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVKY 347
           DEE GDYWIIRNQYGTQWGMNGYMKMQRG RNPQGVCGMA+QP++PVK+
Sbjct: 301 DEEDGDYWIIRNQYGTQWGMNGYMKMQRGARNPQGVCGMAIQPAYPVKH 347

BLAST of CSPI04G03340 vs. NCBI nr
Match: gi|659101492|ref|XP_008451632.1| (PREDICTED: ervatamin-B-like [Cucumis melo])

HSP 1 Score: 584.3 bits (1505), Expect = 1.4e-163
Identity = 278/349 (79.66%), Postives = 314/349 (89.97%), Query Frame = 1

Query: 1   MTMMKFLIVFVVLIAFTSHLCESFELEGKDFESERSLMQLYKRWSSHHRISRNAHEMHKR 60
           M +MKF IV ++LIAF SHLCESFELE KDFESE++LMQLYKRWSSHHRISRNA+EMHKR
Sbjct: 1   MAVMKFFIVPLILIAFMSHLCESFELERKDFESEKNLMQLYKRWSSHHRISRNANEMHKR 60

Query: 61  FKIFQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMYGSNITHYNSLHAN---RVGG 120
           FK+F+DNAK VF+ N MGKSLKL+LNQFAD+SDDEF  ++ SNITHY +LHA    RVGG
Sbjct: 61  FKVFKDNAKQVFKKNQMGKSLKLKLNQFADMSDDEFRSIHSSNITHYKNLHAKTIGRVGG 120

Query: 121 FMYERAMNIPSSIDWRQRGAVNAIKNQGRCGSCWAFAAVAAVESIHQIRTNELVSLSEQE 180
           FMYE A  IPSSIDWR++GAVNAIK+QGRCGSCWAFAAVAAVESIHQI+TNELVSLSEQE
Sbjct: 121 FMYEHAKEIPSSIDWRKKGAVNAIKDQGRCGSCWAFAAVAAVESIHQIKTNELVSLSEQE 180

Query: 181 VVDCDYKVGGCRGGNYDSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNSERVTIDGYE 240
           VVDCDYK GGCRGG+Y+SAFEFIM+N G+T E+NYPYF G+GYCRRRG   ERVTIDGYE
Sbjct: 181 VVDCDYKDGGCRGGHYNSAFEFIMENDGVTAEDNYPYFEGDGYCRRRGGYKERVTIDGYE 240

Query: 241 RVPQNNEYALMKAVAHQPVAVSVASSGSDFRFYGEASGMLREDSFCGYRIDHTVVVVGYG 300
            VP+NNE+ALMKAVAHQPVAV++AS G DF+FYG+  GM  E++FCGY IDHTVVVVGYG
Sbjct: 241 NVPRNNEHALMKAVAHQPVAVAIASGGFDFKFYGQ--GMFTEENFCGYNIDHTVVVVGYG 300

Query: 301 SDEEGDYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVKY 347
           +DE+GDYWIIRNQYGTQWGMNGYMKMQRG RNPQGVCGMAMQP++PVKY
Sbjct: 301 TDEDGDYWIIRNQYGTQWGMNGYMKMQRGARNPQGVCGMAMQPAYPVKY 347

BLAST of CSPI04G03340 vs. NCBI nr
Match: gi|778722411|ref|XP_011658479.1| (PREDICTED: ervatamin-B-like [Cucumis sativus])

HSP 1 Score: 573.2 bits (1476), Expect = 3.2e-160
Identity = 272/353 (77.05%), Postives = 313/353 (88.67%), Query Frame = 1

Query: 1   MTMMKFLIVFVVLIAFTSHLCESFELEGKDFESERSLMQLYKRWSSHHRISRNAHEMHKR 60
           MT+MKFLIV +VL+AF+ ++CESFELE KDFESE+SLMQLYKRWSSHHRISRNA+EMH R
Sbjct: 1   MTVMKFLIVPLVLVAFSCNICESFELERKDFESEKSLMQLYKRWSSHHRISRNANEMHNR 60

Query: 61  FKIFQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMYGSNITHYNSLHAN------- 120
           FK+F++NAKHVF+VN MGKSLKL+LNQFAD+SDDEF  MY SNIT+Y  LHA        
Sbjct: 61  FKVFKNNAKHVFKVNLMGKSLKLKLNQFADMSDDEFRNMYSSNITYYKDLHAKKIEATGG 120

Query: 121 RVGGFMYERAMNIPSSIDWRQRGAVNAIKNQGRCGSCWAFAAVAAVESIHQIRTNELVSL 180
           R+GGFMYE A NIPSSIDWR++GAVNAIKNQGRCGSCWAFAAVAAVESIHQI+TNELVSL
Sbjct: 121 RIGGFMYEHANNIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELVSL 180

Query: 181 SEQEVVDCDYKVGGCRGGNYDSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNSERVTI 240
           SE+EV+DCDY+ GGCRGG Y+SAFEF+M N G+TIE+NYPY+ GNGYCRRRG  ++RV I
Sbjct: 181 SEEEVLDCDYRDGGCRGGFYNSAFEFMMDNDGVTIEDNYPYYEGNGYCRRRGGRNKRVRI 240

Query: 241 DGYERVPQNNEYALMKAVAHQPVAVSVASSGSDFRFYGEASGMLREDSFCGYRIDHTVVV 300
           DGYE VP+NNEYALMKAVAHQPVAV++AS GSDF+FYG   GM  E+ FCG+ IDHTVVV
Sbjct: 241 DGYENVPRNNEYALMKAVAHQPVAVAIASGGSDFKFYG--GGMFTENDFCGFNIDHTVVV 300

Query: 301 VGYGSDEEGDYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVKY 347
           VGYG+DE+GDYWIIRNQYG +WGMNGYMKMQRG  +PQGVCGMAMQP++PVKY
Sbjct: 301 VGYGTDEDGDYWIIRNQYGHRWGMNGYMKMQRGAHSPQGVCGMAMQPAYPVKY 351

BLAST of CSPI04G03340 vs. NCBI nr
Match: gi|700210691|gb|KGN65787.1| (hypothetical protein Csa_1G528520 [Cucumis sativus])

HSP 1 Score: 558.5 bits (1438), Expect = 8.0e-156
Identity = 275/347 (79.25%), Postives = 298/347 (85.88%), Query Frame = 1

Query: 3   MMKFLIVFVVLIAFTSHLCESFELEGKDFESERSLMQLYKRWSSHHRISRNAHEMHKRFK 62
           MMKFLIVF+VLIAF SH+CESFELE KDFESE+SLMQLYKRWSSHHRISRN HEM +RFK
Sbjct: 1   MMKFLIVFLVLIAFISHICESFELERKDFESEKSLMQLYKRWSSHHRISRNEHEMDRRFK 60

Query: 63  IFQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMYGSNITHYNSLHAN---RVGGFM 122
           +F+DNAKHVF+VNHMGKSLKL+LNQFAD+SDDEFS  YGSNIT+Y +LHA    RVGGFM
Sbjct: 61  VFKDNAKHVFKVNHMGKSLKLKLNQFADMSDDEFSKTYGSNITYYKNLHAKVGGRVGGFM 120

Query: 123 YERAMNIPSSIDWRQRGAVNAIKNQGRCGSCWAFAAVAAVESIHQIRTNELVSLSEQEVV 182
           YERA NIPSSIDWR++GA        R   CWAFAAVAAVESIHQIRTNELVSLSEQEVV
Sbjct: 121 YERATNIPSSIDWRKKGA--------RRMCCWAFAAVAAVESIHQIRTNELVSLSEQEVV 180

Query: 183 DCDYKVGGCRGGNYDSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNSERVTIDGYERV 242
           DCDYKVGGCRGG+Y SAFEFIM+NGGIT+E NYPY+AG+GYCRRRGPN+ERVTIDGYE V
Sbjct: 181 DCDYKVGGCRGGDYISAFEFIMENGGITVENNYPYYAGDGYCRRRGPNNERVTIDGYENV 240

Query: 243 PQNNEYALMKAVAHQPVAVSVASSGSDFRFYGEASGMLREDSFCGYRIDHTVVVVGYGSD 302
           P+NNEYALMKAVAHQP                   GM  E++FCG RIDHTVVVVGYGSD
Sbjct: 241 PRNNEYALMKAVAHQP-------------------GMFTEENFCGIRIDHTVVVVGYGSD 300

Query: 303 EEGDYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVKY 347
           EEGDYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAM P+FPVKY
Sbjct: 301 EEGDYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMYPAFPVKY 320

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
CYSEP_RICCO2.5e-9349.43Vignain OS=Ricinus communis GN=CYSEP PE=1 SV=1[more]
CYSEP_VIGMU2.5e-9349.28Vignain OS=Vigna mungo PE=1 SV=1[more]
CYSEP_PHAVU8.2e-9249.13Vignain OS=Phaseolus vulgaris PE=2 SV=2[more]
CEP3_ARATH6.9e-9148.84KDEL-tailed cysteine endopeptidase CEP3 OS=Arabidopsis thaliana GN=CEP3 PE=2 SV=... [more]
CEP2_ARATH1.2e-9049.28KDEL-tailed cysteine endopeptidase CEP2 OS=Arabidopsis thaliana GN=CEP2 PE=1 SV=... [more]
Match NameE-valueIdentityDescription
A0A0A0KGB1_CUCSA2.2e-16077.05Uncharacterized protein OS=Cucumis sativus GN=Csa_6G490180 PE=3 SV=1[more]
A0A0A0LY73_CUCSA5.6e-15679.25Uncharacterized protein OS=Cucumis sativus GN=Csa_1G528520 PE=3 SV=1[more]
A0A0A0KW34_CUCSA7.6e-12195.56Uncharacterized protein OS=Cucumis sativus GN=Csa_4G013580 PE=3 SV=1[more]
A0A0A0LMU4_CUCSA3.8e-10453.71Uncharacterized protein OS=Cucumis sativus GN=Csa_2G349680 PE=3 SV=1[more]
A0A0A0KXE7_CUCSA1.6e-10297.41Uncharacterized protein OS=Cucumis sativus GN=Csa_4G015180 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G48350.13.9e-9248.84 Cysteine proteinases superfamily protein[more]
AT3G48340.16.7e-9249.28 Cysteine proteinases superfamily protein[more]
AT5G50260.18.1e-9047.55 Cysteine proteinases superfamily protein[more]
AT1G20850.11.0e-7644.69 xylem cysteine peptidase 2[more]
AT5G45890.12.8e-7441.38 senescence-associated gene 12[more]
Match NameE-valueIdentityDescription
gi|659108975|ref|XP_008454483.1|3.9e-16681.90PREDICTED: ervatamin-B-like [Cucumis melo][more]
gi|659108973|ref|XP_008454482.1|4.7e-16480.80PREDICTED: ervatamin-B-like [Cucumis melo][more]
gi|659101492|ref|XP_008451632.1|1.4e-16379.66PREDICTED: ervatamin-B-like [Cucumis melo][more]
gi|778722411|ref|XP_011658479.1|3.2e-16077.05PREDICTED: ervatamin-B-like [Cucumis sativus][more]
gi|700210691|gb|KGN65787.1|8.0e-15679.25hypothetical protein Csa_1G528520 [Cucumis sativus][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000169Pept_cys_AS
IPR000668Peptidase_C1A_C
IPR013128Peptidase_C1A
IPR013201Prot_inhib_I29
Vocabulary: Biological Process
TermDefinition
GO:0006508proteolysis
Vocabulary: Molecular Function
TermDefinition
GO:0008234cysteine-type peptidase activity
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0005575 cellular_component
molecular_function GO:0008234 cysteine-type peptidase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI04G03340.1CSPI04G03340.1mRNA


Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000169Cysteine peptidase, cysteine active sitePROSITEPS00139THIOL_PROTEASE_CYScoord: 144..155
scor
IPR000668Peptidase C1A, papain C-terminalPRINTSPR00705PAPAINcoord: 304..310
score: 1.7E-9coord: 289..299
score: 1.7E-9coord: 144..159
score: 1.
IPR000668Peptidase C1A, papain C-terminalPFAMPF00112Peptidase_C1coord: 126..343
score: 1.9
IPR000668Peptidase C1A, papain C-terminalSMARTSM00645pept_c1coord: 126..344
score: 5.6
IPR013128Peptidase C1APANTHERPTHR12411CYSTEINE PROTEASE FAMILY C1-RELATEDcoord: 3..345
score: 4.3E
IPR013201Cathepsin propeptide inhibitor domain (I29)PFAMPF08246Inhibitor_I29coord: 41..96
score: 9.0
IPR013201Cathepsin propeptide inhibitor domain (I29)SMARTSM00848Inhibitor_I29_2coord: 41..96
score: 1.
NoneNo IPR availableGENE3DG3DSA:3.90.70.10coord: 7..344
score: 2.0E
NoneNo IPR availablePANTHERPTHR12411:SF346KDEL-TAILED CYSTEINE ENDOPEPTIDASE CEP1-RELATEDcoord: 3..345
score: 4.3E
NoneNo IPR availableunknownSSF54001Cysteine proteinasescoord: 34..344
score: 1.35