CSPI04G03270 (gene) Wild cucumber (PI 183967)

NameCSPI04G03270
Typegene
OrganismCucumis sativus (Wild cucumber (PI 183967))
DescriptionCysteine protease, putative
LocationChr4 : 2022811 .. 2024155 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACCATGATCAAATTTCTTATCGTTTTTGTTGTTTCGATTGCTTTCACCTCTCACCTATGTGAGAGCTTTGAGTTAGAAAGGAAAGATTTTGAATCCGAAAAAAGCCTCATGCAACTCTACAAGAGATGGAGTAGCCACCATAGAATCTCAAGGAATGCACATGAGATGCACAAACGTTTCAAGATCTGTCAAGATAATGCAAAACATGTGTTTAGAGTGAACCACATGGGAAAATCATTGAAATTGCGGCTTAACCAATTTGCTGATTTGTCTGATGATGAGTTTAGTATGATGTATGGTTCCAACATTACTCACTACGACAACTTACATGCCAAGGCTGGTGGTGGTGGTCGTGTTGGTGGATTTATGTATGAACGAGCAAGGTATATTCCATCTTCAATCGACTGGAGGGAAAAAGGAGCTGTGAATGCCATAAAAAATCAAGGCCTCTGTGGTAATTAATTTAATTTCATTTATAATCTTTCTAAGCCTAATGCCTTCTAACTAATAATTACCAATGTCTTCTAACTAATAATTATGTGTTATTATGTGTTGTTCGACAGGGAGTTGTTGGGCGTTTGCAGCTGTGGCTGCCGTGGAATCTATTCACCAAATAAAAACAAATGAGTTAGTATCTCTATCGGAGCAAGAAGTGGTGGATTGTGATTATAAAGTTGGTGGCTGTCGTGGAGGAGATTATAACTCTGCATTTGAGTTCATAATGCAAAATGGCGGAATCACAATTGAGGAAAACTATCCGTATTTTGCAGGAAACGGATATTGTCGTAGACGAGGAGTGAGTTTAAACCAATATAATTCTAATTAAACTATCTAAATTTATCATATGAGTGCATTATTGATTATAATATAATTATGTGTATTGTGTTTGATATTTCACTAATAGCCTAACAATGAGAGAGTAACAATTGATGGATATGAGCGTGTACCTCAAAACAACGAGTATGCTTTGATGAAAGCAGTGGCACACCAACCGGTAGCGGTGGCTGTAGCGTCAAGTGGAAGTGATTTTAGATTTTACGGGGAGGCAAGTGTAGTTAATTTTGTTGTTGTTAGTTATAATTTGGTGCAACATATATAATTAAAAAATGATGATTATTTGCAGGGAATGCTTAGAGAAGGTAGCTTTTGCGGATATAGAATTGACCACACGGTGGTGGTAGTTGGGTATGGAAGTGATGAAGAGGGAGATTATTGGATAATAAGAAACCAATATGGAACTCAATGGGGAATGAATGGTTATATGAAGATGCAACGAGGAACACGAAACCCACAAGGTGTATGTGGAATGGCAATGCAACCTTCCTTTCCCGTCAAGTATTGA

mRNA sequence

ATGACCATGATCAAATTTCTTATCGTTTTTGTTGTTTCGATTGCTTTCACCTCTCACCTATGTGAGAGCTTTGAGTTAGAAAGGAAAGATTTTGAATCCGAAAAAAGCCTCATGCAACTCTACAAGAGATGGAGTAGCCACCATAGAATCTCAAGGAATGCACATGAGATGCACAAACGTTTCAAGATCTGTCAAGATAATGCAAAACATGTGTTTAGAGTGAACCACATGGGAAAATCATTGAAATTGCGGCTTAACCAATTTGCTGATTTGTCTGATGATGAGTTTAGTATGATGTATGGTTCCAACATTACTCACTACGACAACTTACATGCCAAGGCTGGTGGTGGTGGTCGTGTTGGTGGATTTATGTATGAACGAGCAAGGTATATTCCATCTTCAATCGACTGGAGGGAAAAAGGAGCTGTGAATGCCATAAAAAATCAAGGCCTCTGTGGGAGTTGTTGGGCGTTTGCAGCTGTGGCTGCCGTGGAATCTATTCACCAAATAAAAACAAATGAGTTAGTATCTCTATCGGAGCAAGAAGTGGTGGATTGTGATTATAAAGTTGGTGGCTGTCGTGGAGGAGATTATAACTCTGCATTTGAGTTCATAATGCAAAATGGCGGAATCACAATTGAGGAAAACTATCCGTATTTTGCAGGAAACGGATATTGTCGTAGACGAGGACCTAACAATGAGAGAGTAACAATTGATGGATATGAGCGTGTACCTCAAAACAACGAGTATGCTTTGATGAAAGCAGTGGCACACCAACCGGTAGCGGTGGCTGTAGCGTCAAGTGGAAGTGATTTTAGATTTTACGGGGAGGGAATGCTTAGAGAAGGTAGCTTTTGCGGATATAGAATTGACCACACGGTGGTGGTAGTTGGGTATGGAAGTGATGAAGAGGGAGATTATTGGATAATAAGAAACCAATATGGAACTCAATGGGGAATGAATGGTTATATGAAGATGCAACGAGGAACACGAAACCCACAAGGTGTATGTGGAATGGCAATGCAACCTTCCTTTCCCGTCAAGTATTGA

Coding sequence (CDS)

ATGACCATGATCAAATTTCTTATCGTTTTTGTTGTTTCGATTGCTTTCACCTCTCACCTATGTGAGAGCTTTGAGTTAGAAAGGAAAGATTTTGAATCCGAAAAAAGCCTCATGCAACTCTACAAGAGATGGAGTAGCCACCATAGAATCTCAAGGAATGCACATGAGATGCACAAACGTTTCAAGATCTGTCAAGATAATGCAAAACATGTGTTTAGAGTGAACCACATGGGAAAATCATTGAAATTGCGGCTTAACCAATTTGCTGATTTGTCTGATGATGAGTTTAGTATGATGTATGGTTCCAACATTACTCACTACGACAACTTACATGCCAAGGCTGGTGGTGGTGGTCGTGTTGGTGGATTTATGTATGAACGAGCAAGGTATATTCCATCTTCAATCGACTGGAGGGAAAAAGGAGCTGTGAATGCCATAAAAAATCAAGGCCTCTGTGGGAGTTGTTGGGCGTTTGCAGCTGTGGCTGCCGTGGAATCTATTCACCAAATAAAAACAAATGAGTTAGTATCTCTATCGGAGCAAGAAGTGGTGGATTGTGATTATAAAGTTGGTGGCTGTCGTGGAGGAGATTATAACTCTGCATTTGAGTTCATAATGCAAAATGGCGGAATCACAATTGAGGAAAACTATCCGTATTTTGCAGGAAACGGATATTGTCGTAGACGAGGACCTAACAATGAGAGAGTAACAATTGATGGATATGAGCGTGTACCTCAAAACAACGAGTATGCTTTGATGAAAGCAGTGGCACACCAACCGGTAGCGGTGGCTGTAGCGTCAAGTGGAAGTGATTTTAGATTTTACGGGGAGGGAATGCTTAGAGAAGGTAGCTTTTGCGGATATAGAATTGACCACACGGTGGTGGTAGTTGGGTATGGAAGTGATGAAGAGGGAGATTATTGGATAATAAGAAACCAATATGGAACTCAATGGGGAATGAATGGTTATATGAAGATGCAACGAGGAACACGAAACCCACAAGGTGTATGTGGAATGGCAATGCAACCTTCCTTTCCCGTCAAGTATTGA
BLAST of CSPI04G03270 vs. Swiss-Prot
Match: CYSEP_RICCO (Vignain OS=Ricinus communis GN=CYSEP PE=1 SV=1)

HSP 1 Score: 349.0 bits (894), Expect = 6.1e-95
Identity = 179/350 (51.14%), Postives = 243/350 (69.43%), Query Frame = 1

Query: 3   MIKFLIVFVVSIAFTSHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNAHEMHKRFK 62
           M KF+++  +S+A    + ESF+   K+ ESE+SL  LY+RW SHH +SR+ HE  KRF 
Sbjct: 1   MQKFILL-ALSLALVLAITESFDFHEKELESEESLWGLYERWRSHHTVSRSLHEKQKRFN 60

Query: 63  ICQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMY-GSNITHYDNLHAKAGGGGRVG 122
           + + NA HV   N M K  KL+LN+FAD+++ EF   Y GS + H    H    GG R  
Sbjct: 61  VFKHNAMHVHNANKMDKPYKLKLNKFADMTNHEFRNTYSGSKVKH----HRMFRGGPRGN 120

Query: 123 G-FMYERARYIPSSIDWREKGAVNAIKNQGLCGSCWAFAAVAAVESIHQIKTNELVSLSE 182
           G FMYE+   +P+S+DWR+KGAV ++K+QG CGSCWAF+ + AVE I+QIKTN+LVSLSE
Sbjct: 121 GTFMYEKVDTVPASVDWRKKGAVTSVKDQGQCGSCWAFSTIVAVEGINQIKTNKLVSLSE 180

Query: 183 QEVVDCDYKVG-GCRGGDYNSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNNERVTID 242
           QE+VDCD     GC GG  + AFEFI Q GGIT E NYPY A +G C     N   V+ID
Sbjct: 181 QELVDCDTDQNQGCNGGLMDYAFEFIKQRGGITTEANYPYEAYDGTCDVSKENAPAVSID 240

Query: 243 GYERVPQNNEYALMKAVAHQPVAVAVASSGSDFRFYGEGMLREGSFCGYRIDHTVVVVGY 302
           G+E VP+N+E AL+KAVA+QPV+VA+ + GSDF+FY EG+   GS CG  +DH V +VGY
Sbjct: 241 GHENVPENDENALLKAVANQPVSVAIDAGGSDFQFYSEGVF-TGS-CGTELDHGVAIVGY 300

Query: 303 GSDEEG-DYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVK 349
           G+  +G  YW ++N +G +WG  GY++M+RG  + +G+CG+AM+ S+P+K
Sbjct: 301 GTTIDGTKYWTVKNSWGPEWGEKGYIRMERGISDKEGLCGIAMEASYPIK 343

BLAST of CSPI04G03270 vs. Swiss-Prot
Match: CYSEP_VIGMU (Vignain OS=Vigna mungo PE=1 SV=1)

HSP 1 Score: 346.3 bits (887), Expect = 4.0e-94
Identity = 173/348 (49.71%), Postives = 233/348 (66.95%), Query Frame = 1

Query: 4   IKFLIVFVVSIAFTSHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNAHEMHKRFKI 63
           +K L+  V+S++    +  SF+   KD ESE+SL  LY+RW SHH +SR+  E HKRF +
Sbjct: 3   MKKLLWVVLSLSLVLGVANSFDFHEKDLESEESLWDLYERWRSHHTVSRSLGEKHKRFNV 62

Query: 64  CQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMY-GSNITHYDNLHAKAGGGGRVGG 123
            + N  HV   N M K  KL+LN+FAD+++ EF   Y GS + H+        G G    
Sbjct: 63  FKANVMHVHNTNKMDKPYKLKLNKFADMTNHEFRSTYAGSKVNHHKMFRGSQHGSGT--- 122

Query: 124 FMYERARYIPSSIDWREKGAVNAIKNQGLCGSCWAFAAVAAVESIHQIKTNELVSLSEQE 183
           FMYE+   +P+S+DWR+KGAV  +K+QG CGSCWAF+ + AVE I+QIKTN+LVSLSEQE
Sbjct: 123 FMYEKVGSVPASVDWRKKGAVTDVKDQGQCGSCWAFSTIVAVEGINQIKTNKLVSLSEQE 182

Query: 184 VVDCDYKVG-GCRGGDYNSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNNERVTIDGY 243
           +VDCD +   GC GG   SAFEFI Q GGIT E NYPY A  G C     N+  V+IDG+
Sbjct: 183 LVDCDKEENQGCNGGLMESAFEFIKQKGGITTESNYPYTAQEGTCDESKVNDLAVSIDGH 242

Query: 244 ERVPQNNEYALMKAVAHQPVAVAVASSGSDFRFYGEGMLREGSFCGYRIDHTVVVVGYGS 303
           E VP N+E AL+KAVA+QPV+VA+ + GSDF+FY EG+      C   ++H V +VGYG+
Sbjct: 243 ENVPVNDENALLKAVANQPVSVAIDAGGSDFQFYSEGVFT--GDCNTDLNHGVAIVGYGT 302

Query: 304 DEEG-DYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVK 349
             +G +YWI+RN +G +WG  GY++MQR     +G+CG+AM  S+P+K
Sbjct: 303 TVDGTNYWIVRNSWGPEWGEQGYIRMQRNISKKEGLCGIAMMASYPIK 345

BLAST of CSPI04G03270 vs. Swiss-Prot
Match: CEP2_ARATH (KDEL-tailed cysteine endopeptidase CEP2 OS=Arabidopsis thaliana GN=CEP2 PE=1 SV=1)

HSP 1 Score: 337.4 bits (864), Expect = 1.8e-91
Identity = 177/350 (50.57%), Postives = 233/350 (66.57%), Query Frame = 1

Query: 3   MIKFLIVFVVSIAFTSHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNAHEMHKRFK 62
           M K L++F+ S+      C  F+ + K+ ESE+ L  LY RW SHH + R+ +E  KRF 
Sbjct: 1   MKKLLLIFLFSLVILQTAC-GFDYDDKEIESEEGLSTLYDRWRSHHSVPRSLNEREKRFN 60

Query: 63  ICQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMY-GSNITHYDNLHAKAGGGGRVG 122
           + + N  HV   N   +S KL+LN+FADL+ +EF   Y GSNI H+  L     G  +  
Sbjct: 61  VFRHNVMHVHNTNKKNRSYKLKLNKFADLTINEFKNAYTGSNIKHHRMLQGPKRGSKQ-- 120

Query: 123 GFMY--ERARYIPSSIDWREKGAVNAIKNQGLCGSCWAFAAVAAVESIHQIKTNELVSLS 182
            FMY  E    +PSS+DWR+KGAV  IKNQG CGSCWAF+ VAAVE I++IKTN+LVSLS
Sbjct: 121 -FMYDHENLSKLPSSVDWRKKGAVTEIKNQGKCGSCWAFSTVAAVEGINKIKTNKLVSLS 180

Query: 183 EQEVVDCDYKVG-GCRGGDYNSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNNERVTI 242
           EQE+VDCD K   GC GG    AFEFI +NGGIT E++YPY   +G C     N   VTI
Sbjct: 181 EQELVDCDTKQNEGCNGGLMEIAFEFIKKNGGITTEDSYPYEGIDGKCDASKDNGVLVTI 240

Query: 243 DGYERVPQNNEYALMKAVAHQPVAVAVASSGSDFRFYGEGMLREGSFCGYRIDHTVVVVG 302
           DG+E VP+N+E AL+KAVA+QPV+VA+ +  SDF+FY EG+   GS CG  ++H V  VG
Sbjct: 241 DGHEDVPENDENALLKAVANQPVSVAIDAGSSDFQFYSEGVF-TGS-CGTELNHGVAAVG 300

Query: 303 YGSDEEGDYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVK 349
           YGS+    YWI+RN +G +WG  GY+K++R    P+G CG+AM+ S+P+K
Sbjct: 301 YGSERGKKYWIVRNSWGAEWGEGGYIKIEREIDEPEGRCGIAMEASYPIK 344

BLAST of CSPI04G03270 vs. Swiss-Prot
Match: CYSEP_PHAVU (Vignain OS=Phaseolus vulgaris PE=2 SV=2)

HSP 1 Score: 337.0 bits (863), Expect = 2.4e-91
Identity = 173/347 (49.86%), Postives = 230/347 (66.28%), Query Frame = 1

Query: 5   KFLIVFVVSIAFTSHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNAHEMHKRFKIC 64
           K L+  V+S +    +  SF+   KD  SE+SL  LY+RW SHH +SR+  E HKRF + 
Sbjct: 4   KKLLWVVLSFSLVLGVANSFDFHDKDLASEESLWDLYERWRSHHTVSRSLGEKHKRFNVF 63

Query: 65  QDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMY-GSNITHYDNLHAKAGGGGRVGGF 124
           + N  HV   N M K  KL+LN+FAD+++ EF   Y GS + H        G     G F
Sbjct: 64  KANLMHVHNTNKMDKPYKLKLNKFADMTNHEFRSTYAGSKVNHPRMFR---GTPHENGAF 123

Query: 125 MYERARYIPSSIDWREKGAVNAIKNQGLCGSCWAFAAVAAVESIHQIKTNELVSLSEQEV 184
           MYE+   +P S+DWR+KGAV  +K+QG CGSCWAF+ V AVE I+QIKTN+LV+LSEQE+
Sbjct: 124 MYEKVVSVPPSVDWRKKGAVTDVKDQGQCGSCWAFSTVVAVEGINQIKTNKLVALSEQEL 183

Query: 185 VDCDYKVG-GCRGGDYNSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNNERVTIDGYE 244
           VDCD +   GC GG   SAFEFI Q GGIT E NYPY A  G C     N+  V+IDG+E
Sbjct: 184 VDCDKEENQGCNGGLMESAFEFIKQKGGITTESNYPYKAQEGTCDASKVNDLAVSIDGHE 243

Query: 245 RVPQNNEYALMKAVAHQPVAVAVASSGSDFRFYGEGMLREGSFCGYRIDHTVVVVGYGSD 304
            VP N+E AL+KAVA+QPV+VA+ + GSDF+FY EG+      C   ++H V +VGYG+ 
Sbjct: 244 NVPANDEDALLKAVANQPVSVAIDAGGSDFQFYSEGVFT--GDCSTDLNHGVAIVGYGTT 303

Query: 305 EEG-DYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVK 349
            +G +YWI+RN +G +WG +GY++MQR     +G+CG+AM PS+P+K
Sbjct: 304 VDGTNYWIVRNSWGPEWGEHGYIRMQRNISKKEGLCGIAMLPSYPIK 345

BLAST of CSPI04G03270 vs. Swiss-Prot
Match: CEP3_ARATH (KDEL-tailed cysteine endopeptidase CEP3 OS=Arabidopsis thaliana GN=CEP3 PE=2 SV=1)

HSP 1 Score: 335.9 bits (860), Expect = 5.4e-91
Identity = 171/347 (49.28%), Postives = 232/347 (66.86%), Query Frame = 1

Query: 8   IVFVVSIAFTSHLCES--FELERKDFESEKSLMQLYKRWSSHHRISRNAHEMHKRFKICQ 67
           + F+V I+F S L  S  F+ + K+ E+E+++ +LY+RW  HH +SR +HE  KRF + +
Sbjct: 3   LFFIVLISFLSLLQASKGFDFDEKELETEENVWKLYERWRGHHSVSRASHEAIKRFNVFR 62

Query: 68  DNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMY-GSNITHYDNLHAKAGGGGRVGGFM 127
            N  HV R N   K  KL++N+FAD++  EF   Y GSN+ H+  L     G G   GFM
Sbjct: 63  HNVLHVHRTNKKNKPYKLKINRFADITHHEFRSSYAGSNVKHHRMLRGPKRGSG---GFM 122

Query: 128 YERARYIPSSIDWREKGAVNAIKNQGLCGSCWAFAAVAAVESIHQIKTNELVSLSEQEVV 187
           YE    +PSS+DWREKGAV  +KNQ  CGSCWAF+ VAAVE I++I+TN+LVSLSEQE+V
Sbjct: 123 YENVTRVPSSVDWREKGAVTEVKNQQDCGSCWAFSTVAAVEGINKIRTNKLVSLSEQELV 182

Query: 188 DCDYKVG-GCRGGDYNSAFEFIMQNGGITIEENYPYFAGN-GYCRRRGPNNERVTIDGYE 247
           DCD +   GC GG    AFEFI  NGGI  EE YPY + +  +CR      E VTIDG+E
Sbjct: 183 DCDTEENQGCAGGLMEPAFEFIKNNGGIKTEETYPYDSSDVQFCRANSIGGETVTIDGHE 242

Query: 248 RVPQNNEYALMKAVAHQPVAVAVASSGSDFRFYGEGMLREGSFCGYRIDHTVVVVGYGSD 307
            VP+N+E  L+KAVAHQPV+VA+ +  SDF+ Y EG+      CG +++H VV+VGYG  
Sbjct: 243 HVPENDEEELLKAVAHQPVSVAIDAGSSDFQLYSEGVFI--GECGTQLNHGVVIVGYGET 302

Query: 308 EEG-DYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVK 349
           + G  YWI+RN +G +WG  GY++++RG    +G CG+AM+ S+P K
Sbjct: 303 KNGTKYWIVRNSWGPEWGEGGYVRIERGISENEGRCGIAMEASYPTK 344

BLAST of CSPI04G03270 vs. TrEMBL
Match: A0A0A0KGB1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G490180 PE=3 SV=1)

HSP 1 Score: 583.9 bits (1504), Expect = 1.3e-163
Identity = 277/351 (78.92%), Postives = 313/351 (89.17%), Query Frame = 1

Query: 1   MTMIKFLIVFVVSIAFTSHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNAHEMHKR 60
           MT++KFLIV +V +AF+ ++CESFELERKDFESEKSLMQLYKRWSSHHRISRNA+EMH R
Sbjct: 1   MTVMKFLIVPLVLVAFSCNICESFELERKDFESEKSLMQLYKRWSSHHRISRNANEMHNR 60

Query: 61  FKICQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMYGSNITHYDNLHAKA--GGGG 120
           FK+ ++NAKHVF+VN MGKSLKL+LNQFAD+SDDEF  MY SNIT+Y +LHAK     GG
Sbjct: 61  FKVFKNNAKHVFKVNLMGKSLKLKLNQFADMSDDEFRNMYSSNITYYKDLHAKKIEATGG 120

Query: 121 RVGGFMYERARYIPSSIDWREKGAVNAIKNQGLCGSCWAFAAVAAVESIHQIKTNELVSL 180
           R+GGFMYE A  IPSSIDWR+KGAVNAIKNQG CGSCWAFAAVAAVESIHQIKTNELVSL
Sbjct: 121 RIGGFMYEHANNIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELVSL 180

Query: 181 SEQEVVDCDYKVGGCRGGDYNSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNNERVTI 240
           SE+EV+DCDY+ GGCRGG YNSAFEF+M N G+TIE+NYPY+ GNGYCRRRG  N+RV I
Sbjct: 181 SEEEVLDCDYRDGGCRGGFYNSAFEFMMDNDGVTIEDNYPYYEGNGYCRRRGGRNKRVRI 240

Query: 241 DGYERVPQNNEYALMKAVAHQPVAVAVASSGSDFRFYGEGMLREGSFCGYRIDHTVVVVG 300
           DGYE VP+NNEYALMKAVAHQPVAVA+AS GSDF+FYG GM  E  FCG+ IDHTVVVVG
Sbjct: 241 DGYENVPRNNEYALMKAVAHQPVAVAIASGGSDFKFYGGGMFTENDFCGFNIDHTVVVVG 300

Query: 301 YGSDEEGDYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVKY 350
           YG+DE+GDYWIIRNQYG +WGMNGYMKMQRG  +PQGVCGMAMQP++PVKY
Sbjct: 301 YGTDEDGDYWIIRNQYGHRWGMNGYMKMQRGAHSPQGVCGMAMQPAYPVKY 351

BLAST of CSPI04G03270 vs. TrEMBL
Match: A0A0A0LY73_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G528520 PE=3 SV=1)

HSP 1 Score: 565.1 bits (1455), Expect = 6.0e-158
Identity = 279/347 (80.40%), Postives = 300/347 (86.46%), Query Frame = 1

Query: 3   MIKFLIVFVVSIAFTSHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNAHEMHKRFK 62
           M+KFLIVF+V IAF SH+CESFELERKDFESEKSLMQLYKRWSSHHRISRN HEM +RFK
Sbjct: 1   MMKFLIVFLVLIAFISHICESFELERKDFESEKSLMQLYKRWSSHHRISRNEHEMDRRFK 60

Query: 63  ICQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMYGSNITHYDNLHAKAGGGGRVGG 122
           + +DNAKHVF+VNHMGKSLKL+LNQFAD+SDDEFS  YGSNIT+Y NLHAK GG  RVGG
Sbjct: 61  VFKDNAKHVFKVNHMGKSLKLKLNQFADMSDDEFSKTYGSNITYYKNLHAKVGG--RVGG 120

Query: 123 FMYERARYIPSSIDWREKGAVNAIKNQGLCGSCWAFAAVAAVESIHQIKTNELVSLSEQE 182
           FMYERA  IPSSIDWR+KGA      + +C  CWAFAAVAAVESIHQI+TNELVSLSEQE
Sbjct: 121 FMYERATNIPSSIDWRKKGA------RRMC--CWAFAAVAAVESIHQIRTNELVSLSEQE 180

Query: 183 VVDCDYKVGGCRGGDYNSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNNERVTIDGYE 242
           VVDCDYKVGGCRGGDY SAFEFIM+NGGIT+E NYPY+AG+GYCRRRGPNNERVTIDGYE
Sbjct: 181 VVDCDYKVGGCRGGDYISAFEFIMENGGITVENNYPYYAGDGYCRRRGPNNERVTIDGYE 240

Query: 243 RVPQNNEYALMKAVAHQPVAVAVASSGSDFRFYGEGMLREGSFCGYRIDHTVVVVGYGSD 302
            VP+NNEYALMKAVAHQP                 GM  E +FCG RIDHTVVVVGYGSD
Sbjct: 241 NVPRNNEYALMKAVAHQP-----------------GMFTEENFCGIRIDHTVVVVGYGSD 300

Query: 303 EEGDYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVKY 350
           EEGDYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAM P+FPVKY
Sbjct: 301 EEGDYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMYPAFPVKY 320

BLAST of CSPI04G03270 vs. TrEMBL
Match: A0A0A0KW34_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G013580 PE=3 SV=1)

HSP 1 Score: 424.5 bits (1090), Expect = 1.3e-115
Identity = 211/230 (91.74%), Postives = 216/230 (93.91%), Query Frame = 1

Query: 1   MTMIKFLIVFVVSIAFTSHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNAHEMHKR 60
           MTM+KFLIV VV IAFT+HLCESFELERKDFESEKSLMQLYKRWSSHHRISRNAHEMHKR
Sbjct: 1   MTMMKFLIVLVVLIAFTTHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNAHEMHKR 60

Query: 61  FKICQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMYGSNITHYDNLHAKAGGGGRV 120
           FKI QDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMYGSNITHY+ LHA      RV
Sbjct: 61  FKIFQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMYGSNITHYNGLHAN-----RV 120

Query: 121 GGFMYERARYIPSSIDWREKGAVNAIKNQGLCGSCWAFAAVAAVESIHQIKTNELVSLSE 180
           G FMYERA  IPSSIDWR+KGAVNAIKNQG CGSCWAFAAVAAVESIHQIKTNELVSLSE
Sbjct: 121 GEFMYERAMNIPSSIDWRQKGAVNAIKNQGHCGSCWAFAAVAAVESIHQIKTNELVSLSE 180

Query: 181 QEVVDCDYKVGGCRGGDYNSAFEFIMQNGGITIEENYPYFAGNGYCRRRG 231
           QEVVDCDYKVGGCRGG+YNSAFEFIMQNGGITIEENYPYFAGNGYCRRRG
Sbjct: 181 QEVVDCDYKVGGCRGGNYNSAFEFIMQNGGITIEENYPYFAGNGYCRRRG 225

BLAST of CSPI04G03270 vs. TrEMBL
Match: A0A0A0LMU4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G349680 PE=3 SV=1)

HSP 1 Score: 379.8 bits (974), Expect = 3.6e-102
Identity = 185/351 (52.71%), Postives = 252/351 (71.79%), Query Frame = 1

Query: 1   MTMIKFLIVFVVSIAFTSHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNAHEMHKR 60
           M + KFL+V ++ I   S L ESFE + K+  +E+SL QLY+RW  HH ISRN  E HKR
Sbjct: 1   MAIGKFLLVPLLLIVLVSGLAESFEFDEKELATEESLWQLYERWGKHHTISRNLKEKHKR 60

Query: 61  FKICQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMYG-SNITHYDNLHAKAGGGGR 120
           F + ++N  HVF VN M K  KL+LN+FAD+S+ EF   Y  SNI+HY  LH +  G G 
Sbjct: 61  FSVFKENVNHVFTVNQMDKPYKLKLNKFADMSNYEFVNFYARSNISHYRKLHERRRGAG- 120

Query: 121 VGGFMYERARYIPSSIDWREKGAVNAIKNQGLCGSCWAFAAVAAVESIHQIKTNELVSLS 180
             GFMYE+   +PSS+DWRE+GAVNA+K QG CGSCWAF++VAAVE I++IKTN+L+SLS
Sbjct: 121 --GFMYEQDTDLPSSVDWRERGAVNAVKEQGRCGSCWAFSSVAAVEGINKIKTNQLLSLS 180

Query: 181 EQEVVDCDYKVGGCRGGDYNSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNNERVTID 240
           EQE++DC+Y+  GC GG    AF+FI +NGGI  E +YPY    G CR    ++  V ID
Sbjct: 181 EQELLDCNYRNKGCNGGFMEIAFDFIKRNGGIATENSYPYHGSRGLCRSSRISSPIVKID 240

Query: 241 GYERVPQNNEYALMKAVAHQPVAVAVASSGSDFRFYGEGMLREGSFCGYRIDHTVVVVGY 300
           GYE VP+ NE ALM+AVA+QPV+VA+ ++G DF+FY +G+     +CG  ++H VV +GY
Sbjct: 241 GYESVPE-NEDALMQAVANQPVSVAIDAAGRDFQFYSQGVF--DGYCGTELNHGVVAIGY 300

Query: 301 GSDEEG-DYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVKY 350
           G+ E+G DYW++RN +G  WG +GY++M+RG    +G+CG+AM+ S+P+KY
Sbjct: 301 GTTEDGTDYWLVRNSWGVGWGEDGYVRMKRGVEQAEGLCGIAMEASYPIKY 345

BLAST of CSPI04G03270 vs. TrEMBL
Match: V4LTA3_EUTSA (Uncharacterized protein OS=Eutrema salsugineum GN=EUTSA_v10010486mg PE=3 SV=1)

HSP 1 Score: 354.8 bits (909), Expect = 1.2e-94
Identity = 182/349 (52.15%), Postives = 239/349 (68.48%), Query Frame = 1

Query: 3   MIKFLIVFVVSIAFTSHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNAHEMHKRFK 62
           M K L++F+ S      +   F+   K+ ESE+SL +LY+RW SHH + R+ HE  KRF 
Sbjct: 1   MKKLLLIFLSSFLILQTV-RGFDYHDKELESEESLSKLYERWRSHHSVPRSLHEREKRFN 60

Query: 63  ICQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMY-GSNITHYDNLHAKAGGGGRVG 122
           + + N  HV + N   K  KL+LN+FADL+  EF   Y GSNI H+  L     G  R  
Sbjct: 61  VFRHNVMHVHKTNKKNKPYKLKLNKFADLTVHEFKNAYTGSNIKHHRMLQGLKRGSKR-- 120

Query: 123 GFMYERARYIPSSIDWREKGAVNAIKNQGLCGSCWAFAAVAAVESIHQIKTNELVSLSEQ 182
            FMYE +  +PSS+DWREKGAV  +KNQG CGSCWAF+ VAAVE I++IKTN+LVSLSEQ
Sbjct: 121 -FMYENSTRVPSSVDWREKGAVTEVKNQGQCGSCWAFSTVAAVEGINKIKTNKLVSLSEQ 180

Query: 183 EVVDCD-YKVGGCRGGDYNSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNNERVTIDG 242
           E+VDCD Y+  GC GG    AFEFI +NGGIT E+NYPY   +G C     N E VTIDG
Sbjct: 181 ELVDCDTYQNEGCNGGLMEIAFEFIKKNGGITTEDNYPYEGIDGKCDASKDNGEVVTIDG 240

Query: 243 YERVPQNNEYALMKAVAHQPVAVAVASSGSDFRFYGEGMLREGSFCGYRIDHTVVVVGYG 302
           +E VP+N+E AL+KAVA+QPV+VA+ +  S+F+FY EG+     +CG  ++H V VVGYG
Sbjct: 241 HENVPENDEDALLKAVANQPVSVAIDAGSSNFQFYSEGVFT--GYCGTELNHGVAVVGYG 300

Query: 303 SDEEG-DYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVK 349
           SD +G  YWI+RN +G +WG  GY+K++RG  + +G CG+AM+ S+PVK
Sbjct: 301 SDSKGRKYWIVRNSWGPEWGEGGYIKLERGIDDSEGRCGIAMEASYPVK 343

BLAST of CSPI04G03270 vs. TAIR10
Match: AT3G48340.1 (AT3G48340.1 Cysteine proteinases superfamily protein)

HSP 1 Score: 337.4 bits (864), Expect = 1.0e-92
Identity = 177/350 (50.57%), Postives = 233/350 (66.57%), Query Frame = 1

Query: 3   MIKFLIVFVVSIAFTSHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNAHEMHKRFK 62
           M K L++F+ S+      C  F+ + K+ ESE+ L  LY RW SHH + R+ +E  KRF 
Sbjct: 1   MKKLLLIFLFSLVILQTAC-GFDYDDKEIESEEGLSTLYDRWRSHHSVPRSLNEREKRFN 60

Query: 63  ICQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMY-GSNITHYDNLHAKAGGGGRVG 122
           + + N  HV   N   +S KL+LN+FADL+ +EF   Y GSNI H+  L     G  +  
Sbjct: 61  VFRHNVMHVHNTNKKNRSYKLKLNKFADLTINEFKNAYTGSNIKHHRMLQGPKRGSKQ-- 120

Query: 123 GFMY--ERARYIPSSIDWREKGAVNAIKNQGLCGSCWAFAAVAAVESIHQIKTNELVSLS 182
            FMY  E    +PSS+DWR+KGAV  IKNQG CGSCWAF+ VAAVE I++IKTN+LVSLS
Sbjct: 121 -FMYDHENLSKLPSSVDWRKKGAVTEIKNQGKCGSCWAFSTVAAVEGINKIKTNKLVSLS 180

Query: 183 EQEVVDCDYKVG-GCRGGDYNSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNNERVTI 242
           EQE+VDCD K   GC GG    AFEFI +NGGIT E++YPY   +G C     N   VTI
Sbjct: 181 EQELVDCDTKQNEGCNGGLMEIAFEFIKKNGGITTEDSYPYEGIDGKCDASKDNGVLVTI 240

Query: 243 DGYERVPQNNEYALMKAVAHQPVAVAVASSGSDFRFYGEGMLREGSFCGYRIDHTVVVVG 302
           DG+E VP+N+E AL+KAVA+QPV+VA+ +  SDF+FY EG+   GS CG  ++H V  VG
Sbjct: 241 DGHEDVPENDENALLKAVANQPVSVAIDAGSSDFQFYSEGVF-TGS-CGTELNHGVAAVG 300

Query: 303 YGSDEEGDYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVK 349
           YGS+    YWI+RN +G +WG  GY+K++R    P+G CG+AM+ S+P+K
Sbjct: 301 YGSERGKKYWIVRNSWGAEWGEGGYIKIEREIDEPEGRCGIAMEASYPIK 344

BLAST of CSPI04G03270 vs. TAIR10
Match: AT3G48350.1 (AT3G48350.1 Cysteine proteinases superfamily protein)

HSP 1 Score: 335.9 bits (860), Expect = 3.0e-92
Identity = 171/347 (49.28%), Postives = 232/347 (66.86%), Query Frame = 1

Query: 8   IVFVVSIAFTSHLCES--FELERKDFESEKSLMQLYKRWSSHHRISRNAHEMHKRFKICQ 67
           + F+V I+F S L  S  F+ + K+ E+E+++ +LY+RW  HH +SR +HE  KRF + +
Sbjct: 3   LFFIVLISFLSLLQASKGFDFDEKELETEENVWKLYERWRGHHSVSRASHEAIKRFNVFR 62

Query: 68  DNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMY-GSNITHYDNLHAKAGGGGRVGGFM 127
            N  HV R N   K  KL++N+FAD++  EF   Y GSN+ H+  L     G G   GFM
Sbjct: 63  HNVLHVHRTNKKNKPYKLKINRFADITHHEFRSSYAGSNVKHHRMLRGPKRGSG---GFM 122

Query: 128 YERARYIPSSIDWREKGAVNAIKNQGLCGSCWAFAAVAAVESIHQIKTNELVSLSEQEVV 187
           YE    +PSS+DWREKGAV  +KNQ  CGSCWAF+ VAAVE I++I+TN+LVSLSEQE+V
Sbjct: 123 YENVTRVPSSVDWREKGAVTEVKNQQDCGSCWAFSTVAAVEGINKIRTNKLVSLSEQELV 182

Query: 188 DCDYKVG-GCRGGDYNSAFEFIMQNGGITIEENYPYFAGN-GYCRRRGPNNERVTIDGYE 247
           DCD +   GC GG    AFEFI  NGGI  EE YPY + +  +CR      E VTIDG+E
Sbjct: 183 DCDTEENQGCAGGLMEPAFEFIKNNGGIKTEETYPYDSSDVQFCRANSIGGETVTIDGHE 242

Query: 248 RVPQNNEYALMKAVAHQPVAVAVASSGSDFRFYGEGMLREGSFCGYRIDHTVVVVGYGSD 307
            VP+N+E  L+KAVAHQPV+VA+ +  SDF+ Y EG+      CG +++H VV+VGYG  
Sbjct: 243 HVPENDEEELLKAVAHQPVSVAIDAGSSDFQLYSEGVFI--GECGTQLNHGVVIVGYGET 302

Query: 308 EEG-DYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVK 349
           + G  YWI+RN +G +WG  GY++++RG    +G CG+AM+ S+P K
Sbjct: 303 KNGTKYWIVRNSWGPEWGEGGYVRIERGISENEGRCGIAMEASYPTK 344

BLAST of CSPI04G03270 vs. TAIR10
Match: AT5G50260.1 (AT5G50260.1 Cysteine proteinases superfamily protein)

HSP 1 Score: 319.7 bits (818), Expect = 2.2e-87
Identity = 163/348 (46.84%), Postives = 223/348 (64.08%), Query Frame = 1

Query: 4   IKFLIVFVVSIAFTSHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNAHEMHKRFKI 63
           +K  IV  + +       +  +   KD ESE SL +LY+RW SHH ++R+  E  KRF +
Sbjct: 1   MKRFIVLALCMLMVLETTKGLDFHNKDVESENSLWELYERWRSHHTVARSLEEKAKRFNV 60

Query: 64  CQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMY-GSNITHYDNLHAKAGGGGRVGG 123
            + N KH+   N   KS KL+LN+F D++ +EF   Y GSNI H+       G       
Sbjct: 61  FKHNVKHIHETNKKDKSYKLKLNKFGDMTSEEFRRTYAGSNIKHHRMFQ---GEKKATKS 120

Query: 124 FMYERARYIPSSIDWREKGAVNAIKNQGLCGSCWAFAAVAAVESIHQIKTNELVSLSEQE 183
           FMY     +P+S+DWR+ GAV  +KNQG CGSCWAF+ V AVE I+QI+T +L SLSEQE
Sbjct: 121 FMYANVNTLPTSVDWRKNGAVTPVKNQGQCGSCWAFSTVVAVEGINQIRTKKLTSLSEQE 180

Query: 184 VVDCDYKVG-GCRGGDYNSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNNERVTIDGY 243
           +VDCD     GC GG  + AFEFI + GG+T E  YPY A +  C     N   V+IDG+
Sbjct: 181 LVDCDTNQNQGCNGGLMDLAFEFIKEKGGLTSELVYPYKASDETCDTNKENAPVVSIDGH 240

Query: 244 ERVPQNNEYALMKAVAHQPVAVAVASSGSDFRFYGEGMLREGSFCGYRIDHTVVVVGYGS 303
           E VP+N+E  LMKAVA+QPV+VA+ + GSDF+FY EG+      CG  ++H V VVGYG+
Sbjct: 241 EDVPKNSEDDLMKAVANQPVSVAIDAGGSDFQFYSEGVFT--GRCGTELNHGVAVVGYGT 300

Query: 304 DEEG-DYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVK 349
             +G  YWI++N +G +WG  GY++MQRG R+ +G+CG+AM+ S+P+K
Sbjct: 301 TIDGTKYWIVKNSWGEEWGEKGYIRMQRGIRHKEGLCGIAMEASYPLK 343

BLAST of CSPI04G03270 vs. TAIR10
Match: AT1G20850.1 (AT1G20850.1 xylem cysteine peptidase 2)

HSP 1 Score: 282.3 bits (721), Expect = 4.0e-76
Identity = 147/343 (42.86%), Postives = 212/343 (61.81%), Query Frame = 1

Query: 12  VSIAF-TSHLCESFELERKDFESEKSLMQLYKRW-SSHHRISRNAHEMHKRFKICQDNAK 71
           +S++F +SH         +D ES   L++L++ W S+  +      E   RF++ +DN K
Sbjct: 21  LSLSFASSHDYSIVGYSPEDLESHDKLIELFENWISNFEKAYETVEEKFLRFEVFKDNLK 80

Query: 72  HVFRVNHMGKSLKLRLNQFADLSDDEFSMMY---GSNITHYDNLHAKAGGGGRVGGFMYE 131
           H+   N  GKS  L LN+FADLS +EF  MY    ++I   D   + A        F Y 
Sbjct: 81  HIDETNKKGKSYWLGLNEFADLSHEEFKKMYLGLKTDIVRRDEERSYAE-------FAYR 140

Query: 132 RARYIPSSIDWREKGAVNAIKNQGLCGSCWAFAAVAAVESIHQIKTNELVSLSEQEVVDC 191
               +P S+DWR+KGAV  +KNQG CGSCWAF+ VAAVE I++I T  L +LSEQE++DC
Sbjct: 141 DVEAVPKSVDWRKKGAVAEVKNQGSCGSCWAFSTVAAVEGINKIVTGNLTTLSEQELIDC 200

Query: 192 DYKVG-GCRGGDYNSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNNERVTIDGYERVP 251
           D     GC GG  + AFE+I++NGG+  EE+YPY    G C  +   +E VTI+G++ VP
Sbjct: 201 DTTYNNGCNGGLMDYAFEYIVKNGGLRKEEDYPYSMEEGTCEMQKDESETVTINGHQDVP 260

Query: 252 QNNEYALMKAVAHQPVAVAVASSGSDFRFYGEGMLREGSFCGYRIDHTVVVVGYGSDEEG 311
            N+E +L+KA+AHQP++VA+ +SG +F+FY  G+      CG  +DH V  VGYGS +  
Sbjct: 261 TNDEKSLLKALAHQPLSVAIDASGREFQFYSGGVF--DGRCGVDLDHGVAAVGYGSSKGS 320

Query: 312 DYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVK 349
           DY I++N +G +WG  GY++++R T  P+G+CG+    SFP K
Sbjct: 321 DYIIVKNSWGPKWGEKGYIRLKRNTGKPEGLCGINKMASFPTK 354

BLAST of CSPI04G03270 vs. TAIR10
Match: AT3G19400.1 (AT3G19400.1 Cysteine proteinases superfamily protein)

HSP 1 Score: 275.8 bits (704), Expect = 3.7e-74
Identity = 156/347 (44.96%), Postives = 217/347 (62.54%), Query Frame = 1

Query: 8   IVFVVSIAFTSHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNA-HEMHKRFKICQD 67
           +V +  +  +S L  + E E +  E+E  LM  Y++W   +R + N   E  +RFKI +D
Sbjct: 13  LVILSVLLLSSSLGVATETEIERNETEVRLM--YEQWLVENRKNYNGLGEKERRFKIFKD 72

Query: 68  NAKHVFRVNHM-GKSLKLRLNQFADLSDDEFSMMYGSNITHYDNLHAKAGGGGRVGGFMY 127
           N K V   N +  ++ ++ L +FADL+++EF  +Y            +     +   ++Y
Sbjct: 73  NLKFVDEHNSVPDRTFEVGLTRFADLTNEEFRAIY------LRKKMERTKDSVKTERYLY 132

Query: 128 ERARYIPSSIDWREKGAVNAIKNQGLCGSCWAFAAVAAVESIHQIKTNELVSLSEQEVVD 187
           +    +P  +DWR  GAV ++K+QG CGSCWAF+AV AVE I+QI T EL+SLSEQE+VD
Sbjct: 133 KEGDVLPDEVDWRANGAVVSVKDQGNCGSCWAFSAVGAVEGINQITTGELISLSEQELVD 192

Query: 188 CD--YKVGGCRGGDYNSAFEFIMQNGGITIEENYPYFAGN-GYCRRRGPNNER-VTIDGY 247
           CD  +   GC GG  N AFEFIM+NGGI  +++YPY A + G C     NN R VTIDGY
Sbjct: 193 CDRGFVNAGCDGGIMNYAFEFIMKNGGIETDQDYPYNANDLGLCNADKNNNTRVVTIDGY 252

Query: 248 ERVPQNNEYALMKAVAHQPVAVAVASSGSDFRFYGEGMLREGSFCGYRIDHTVVVVGYGS 307
           E VP+++E +L KAVAHQPV+VA+ +S   F+ Y  G++     CG  +DH VVVVGYGS
Sbjct: 253 EDVPRDDEKSLKKAVAHQPVSVAIEASSQAFQLYKSGVMT--GTCGISLDHGVVVVGYGS 312

Query: 308 DEEGDYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVK 349
               DYWIIRN +G  WG +GY+K+QR   +P G CG+AM PS+P K
Sbjct: 313 TSGEDYWIIRNSWGLNWGDSGYVKLQRNIDDPFGKCGIAMMPSYPTK 349

BLAST of CSPI04G03270 vs. NCBI nr
Match: gi|659108975|ref|XP_008454483.1| (PREDICTED: ervatamin-B-like [Cucumis melo])

HSP 1 Score: 607.1 bits (1564), Expect = 2.0e-170
Identity = 292/349 (83.67%), Postives = 320/349 (91.69%), Query Frame = 1

Query: 2   TMIKFLIVFVVSIAFTSHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNAHEMHKRF 61
           T++KFLIV +V IA TSHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNA+EMHKRF
Sbjct: 3   TVMKFLIVPLVLIALTSHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNANEMHKRF 62

Query: 62  KICQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMYGSNITHYDNLHAKAGGGGRVG 121
           K+ +DNAKHVF+ NHMG+SLKL+LNQFAD+SDDEFS ++GSNIT+Y NLHAK G    VG
Sbjct: 63  KVFKDNAKHVFKKNHMGRSLKLQLNQFADMSDDEFSSIHGSNITYYKNLHAKTG---HVG 122

Query: 122 GFMYERARYIPSSIDWREKGAVNAIKNQGLCGSCWAFAAVAAVESIHQIKTNELVSLSEQ 181
           GFMYE A+ IPSSIDWR+KGAVNAIKNQG CGSCWAFAAVAAVESIHQIKTNELVSLSEQ
Sbjct: 123 GFMYEHAKEIPSSIDWRKKGAVNAIKNQGGCGSCWAFAAVAAVESIHQIKTNELVSLSEQ 182

Query: 182 EVVDCDYKVGGCRGGDYNSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNNERVTIDGY 241
           EVVDCDY+ GGCRGG YNSAFEF+M+NGGIT+E+NYPY+ G+GYCRRRG  NERV IDGY
Sbjct: 183 EVVDCDYRDGGCRGGHYNSAFEFMMENGGITVEDNYPYYEGDGYCRRRGGYNERVKIDGY 242

Query: 242 ERVPQNNEYALMKAVAHQPVAVAVASSGSDFRFYGEGMLREGSFCGYRIDHTVVVVGYGS 301
           E VP+NNE+ALMKAVAHQPVAVA+ASSGSDFRFYG+GM  E  FCGY IDHTVVVVGYGS
Sbjct: 243 ENVPRNNEHALMKAVAHQPVAVAIASSGSDFRFYGQGMFTEQDFCGYNIDHTVVVVGYGS 302

Query: 302 DEE-GDYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVKY 350
           DEE GDYWIIRNQYGTQWGMNGYMKMQRG RNPQGVCGMAMQP++PVKY
Sbjct: 303 DEEDGDYWIIRNQYGTQWGMNGYMKMQRGARNPQGVCGMAMQPAYPVKY 348

BLAST of CSPI04G03270 vs. NCBI nr
Match: gi|659108973|ref|XP_008454482.1| (PREDICTED: ervatamin-B-like [Cucumis melo])

HSP 1 Score: 597.4 bits (1539), Expect = 1.6e-167
Identity = 288/350 (82.29%), Postives = 319/350 (91.14%), Query Frame = 1

Query: 1   MTMIKFLIVFVVSIAFTSHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNAHEMHKR 60
           M ++KFLIV +V IAFT HLCESFELERKDFESEKSLMQLYKRWSSHHRISRNA+EMHKR
Sbjct: 1   MAVMKFLIVPLVLIAFTFHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNANEMHKR 60

Query: 61  FKICQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMYGSNITHYDNLHAKAGGGGRV 120
           FK+ +DNAK+VF+ NHMG+SLKL+LNQFAD+SDDEFS ++GSNIT+Y NLHAK    GRV
Sbjct: 61  FKVFKDNAKYVFKKNHMGRSLKLQLNQFADMSDDEFSSIHGSNITYYKNLHAK---NGRV 120

Query: 121 GGFMYERARYIPSSIDWREKGAVNAIKNQGLCGSCWAFAAVAAVESIHQIKTNELVSLSE 180
           GGFMYE A  IPSSIDWR+KGAVNAIKNQG CGSCWAFAAVAAVESIHQIKTNELVSLSE
Sbjct: 121 GGFMYEHANDIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELVSLSE 180

Query: 181 QEVVDCDYKVGGCRGGDYNSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNNERVTIDG 240
           QEVVDCDY+  GC GG YNSAFEF+M+NGGIT+E+NYPY+ G+GYCRRRG  NERVTIDG
Sbjct: 181 QEVVDCDYRDSGCLGGFYNSAFEFMMENGGITVEDNYPYYEGDGYCRRRGGYNERVTIDG 240

Query: 241 YERVPQNNEYALMKAVAHQPVAVAVASSGSDFRFYGEGMLREGSFCGYRIDHTVVVVGYG 300
           YE VP+NNE+ALMKAVAHQPVAVA+ASSGSDFRFYG+GM  E  FCGY IDHTVVVVGYG
Sbjct: 241 YENVPRNNEHALMKAVAHQPVAVAIASSGSDFRFYGQGMFTEQDFCGYNIDHTVVVVGYG 300

Query: 301 SDEE-GDYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVKY 350
           +DEE GDYWIIRNQYGTQWGMNGYMKMQRG RNPQGVCGMA+QP++PVK+
Sbjct: 301 TDEEDGDYWIIRNQYGTQWGMNGYMKMQRGARNPQGVCGMAIQPAYPVKH 347

BLAST of CSPI04G03270 vs. NCBI nr
Match: gi|659101492|ref|XP_008451632.1| (PREDICTED: ervatamin-B-like [Cucumis melo])

HSP 1 Score: 592.8 bits (1527), Expect = 3.9e-166
Identity = 283/349 (81.09%), Postives = 313/349 (89.68%), Query Frame = 1

Query: 1   MTMIKFLIVFVVSIAFTSHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNAHEMHKR 60
           M ++KF IV ++ IAF SHLCESFELERKDFESEK+LMQLYKRWSSHHRISRNA+EMHKR
Sbjct: 1   MAVMKFFIVPLILIAFMSHLCESFELERKDFESEKNLMQLYKRWSSHHRISRNANEMHKR 60

Query: 61  FKICQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMYGSNITHYDNLHAKAGGGGRV 120
           FK+ +DNAK VF+ N MGKSLKL+LNQFAD+SDDEF  ++ SNITHY NLHAK  G  RV
Sbjct: 61  FKVFKDNAKQVFKKNQMGKSLKLKLNQFADMSDDEFRSIHSSNITHYKNLHAKTIG--RV 120

Query: 121 GGFMYERARYIPSSIDWREKGAVNAIKNQGLCGSCWAFAAVAAVESIHQIKTNELVSLSE 180
           GGFMYE A+ IPSSIDWR+KGAVNAIK+QG CGSCWAFAAVAAVESIHQIKTNELVSLSE
Sbjct: 121 GGFMYEHAKEIPSSIDWRKKGAVNAIKDQGRCGSCWAFAAVAAVESIHQIKTNELVSLSE 180

Query: 181 QEVVDCDYKVGGCRGGDYNSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNNERVTIDG 240
           QEVVDCDYK GGCRGG YNSAFEFIM+N G+T E+NYPYF G+GYCRRRG   ERVTIDG
Sbjct: 181 QEVVDCDYKDGGCRGGHYNSAFEFIMENDGVTAEDNYPYFEGDGYCRRRGGYKERVTIDG 240

Query: 241 YERVPQNNEYALMKAVAHQPVAVAVASSGSDFRFYGEGMLREGSFCGYRIDHTVVVVGYG 300
           YE VP+NNE+ALMKAVAHQPVAVA+AS G DF+FYG+GM  E +FCGY IDHTVVVVGYG
Sbjct: 241 YENVPRNNEHALMKAVAHQPVAVAIASGGFDFKFYGQGMFTEENFCGYNIDHTVVVVGYG 300

Query: 301 SDEEGDYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVKY 350
           +DE+GDYWIIRNQYGTQWGMNGYMKMQRG RNPQGVCGMAMQP++PVKY
Sbjct: 301 TDEDGDYWIIRNQYGTQWGMNGYMKMQRGARNPQGVCGMAMQPAYPVKY 347

BLAST of CSPI04G03270 vs. NCBI nr
Match: gi|778722411|ref|XP_011658479.1| (PREDICTED: ervatamin-B-like [Cucumis sativus])

HSP 1 Score: 583.9 bits (1504), Expect = 1.8e-163
Identity = 277/351 (78.92%), Postives = 313/351 (89.17%), Query Frame = 1

Query: 1   MTMIKFLIVFVVSIAFTSHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNAHEMHKR 60
           MT++KFLIV +V +AF+ ++CESFELERKDFESEKSLMQLYKRWSSHHRISRNA+EMH R
Sbjct: 1   MTVMKFLIVPLVLVAFSCNICESFELERKDFESEKSLMQLYKRWSSHHRISRNANEMHNR 60

Query: 61  FKICQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMYGSNITHYDNLHAKA--GGGG 120
           FK+ ++NAKHVF+VN MGKSLKL+LNQFAD+SDDEF  MY SNIT+Y +LHAK     GG
Sbjct: 61  FKVFKNNAKHVFKVNLMGKSLKLKLNQFADMSDDEFRNMYSSNITYYKDLHAKKIEATGG 120

Query: 121 RVGGFMYERARYIPSSIDWREKGAVNAIKNQGLCGSCWAFAAVAAVESIHQIKTNELVSL 180
           R+GGFMYE A  IPSSIDWR+KGAVNAIKNQG CGSCWAFAAVAAVESIHQIKTNELVSL
Sbjct: 121 RIGGFMYEHANNIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELVSL 180

Query: 181 SEQEVVDCDYKVGGCRGGDYNSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNNERVTI 240
           SE+EV+DCDY+ GGCRGG YNSAFEF+M N G+TIE+NYPY+ GNGYCRRRG  N+RV I
Sbjct: 181 SEEEVLDCDYRDGGCRGGFYNSAFEFMMDNDGVTIEDNYPYYEGNGYCRRRGGRNKRVRI 240

Query: 241 DGYERVPQNNEYALMKAVAHQPVAVAVASSGSDFRFYGEGMLREGSFCGYRIDHTVVVVG 300
           DGYE VP+NNEYALMKAVAHQPVAVA+AS GSDF+FYG GM  E  FCG+ IDHTVVVVG
Sbjct: 241 DGYENVPRNNEYALMKAVAHQPVAVAIASGGSDFKFYGGGMFTENDFCGFNIDHTVVVVG 300

Query: 301 YGSDEEGDYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVKY 350
           YG+DE+GDYWIIRNQYG +WGMNGYMKMQRG  +PQGVCGMAMQP++PVKY
Sbjct: 301 YGTDEDGDYWIIRNQYGHRWGMNGYMKMQRGAHSPQGVCGMAMQPAYPVKY 351

BLAST of CSPI04G03270 vs. NCBI nr
Match: gi|700210691|gb|KGN65787.1| (hypothetical protein Csa_1G528520 [Cucumis sativus])

HSP 1 Score: 565.1 bits (1455), Expect = 8.7e-158
Identity = 279/347 (80.40%), Postives = 300/347 (86.46%), Query Frame = 1

Query: 3   MIKFLIVFVVSIAFTSHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNAHEMHKRFK 62
           M+KFLIVF+V IAF SH+CESFELERKDFESEKSLMQLYKRWSSHHRISRN HEM +RFK
Sbjct: 1   MMKFLIVFLVLIAFISHICESFELERKDFESEKSLMQLYKRWSSHHRISRNEHEMDRRFK 60

Query: 63  ICQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMYGSNITHYDNLHAKAGGGGRVGG 122
           + +DNAKHVF+VNHMGKSLKL+LNQFAD+SDDEFS  YGSNIT+Y NLHAK GG  RVGG
Sbjct: 61  VFKDNAKHVFKVNHMGKSLKLKLNQFADMSDDEFSKTYGSNITYYKNLHAKVGG--RVGG 120

Query: 123 FMYERARYIPSSIDWREKGAVNAIKNQGLCGSCWAFAAVAAVESIHQIKTNELVSLSEQE 182
           FMYERA  IPSSIDWR+KGA      + +C  CWAFAAVAAVESIHQI+TNELVSLSEQE
Sbjct: 121 FMYERATNIPSSIDWRKKGA------RRMC--CWAFAAVAAVESIHQIRTNELVSLSEQE 180

Query: 183 VVDCDYKVGGCRGGDYNSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNNERVTIDGYE 242
           VVDCDYKVGGCRGGDY SAFEFIM+NGGIT+E NYPY+AG+GYCRRRGPNNERVTIDGYE
Sbjct: 181 VVDCDYKVGGCRGGDYISAFEFIMENGGITVENNYPYYAGDGYCRRRGPNNERVTIDGYE 240

Query: 243 RVPQNNEYALMKAVAHQPVAVAVASSGSDFRFYGEGMLREGSFCGYRIDHTVVVVGYGSD 302
            VP+NNEYALMKAVAHQP                 GM  E +FCG RIDHTVVVVGYGSD
Sbjct: 241 NVPRNNEYALMKAVAHQP-----------------GMFTEENFCGIRIDHTVVVVGYGSD 300

Query: 303 EEGDYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVKY 350
           EEGDYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAM P+FPVKY
Sbjct: 301 EEGDYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMYPAFPVKY 320

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
CYSEP_RICCO6.1e-9551.14Vignain OS=Ricinus communis GN=CYSEP PE=1 SV=1[more]
CYSEP_VIGMU4.0e-9449.71Vignain OS=Vigna mungo PE=1 SV=1[more]
CEP2_ARATH1.8e-9150.57KDEL-tailed cysteine endopeptidase CEP2 OS=Arabidopsis thaliana GN=CEP2 PE=1 SV=... [more]
CYSEP_PHAVU2.4e-9149.86Vignain OS=Phaseolus vulgaris PE=2 SV=2[more]
CEP3_ARATH5.4e-9149.28KDEL-tailed cysteine endopeptidase CEP3 OS=Arabidopsis thaliana GN=CEP3 PE=2 SV=... [more]
Match NameE-valueIdentityDescription
A0A0A0KGB1_CUCSA1.3e-16378.92Uncharacterized protein OS=Cucumis sativus GN=Csa_6G490180 PE=3 SV=1[more]
A0A0A0LY73_CUCSA6.0e-15880.40Uncharacterized protein OS=Cucumis sativus GN=Csa_1G528520 PE=3 SV=1[more]
A0A0A0KW34_CUCSA1.3e-11591.74Uncharacterized protein OS=Cucumis sativus GN=Csa_4G013580 PE=3 SV=1[more]
A0A0A0LMU4_CUCSA3.6e-10252.71Uncharacterized protein OS=Cucumis sativus GN=Csa_2G349680 PE=3 SV=1[more]
V4LTA3_EUTSA1.2e-9452.15Uncharacterized protein OS=Eutrema salsugineum GN=EUTSA_v10010486mg PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G48340.11.0e-9250.57 Cysteine proteinases superfamily protein[more]
AT3G48350.13.0e-9249.28 Cysteine proteinases superfamily protein[more]
AT5G50260.12.2e-8746.84 Cysteine proteinases superfamily protein[more]
AT1G20850.14.0e-7642.86 xylem cysteine peptidase 2[more]
AT3G19400.13.7e-7444.96 Cysteine proteinases superfamily protein[more]
Match NameE-valueIdentityDescription
gi|659108975|ref|XP_008454483.1|2.0e-17083.67PREDICTED: ervatamin-B-like [Cucumis melo][more]
gi|659108973|ref|XP_008454482.1|1.6e-16782.29PREDICTED: ervatamin-B-like [Cucumis melo][more]
gi|659101492|ref|XP_008451632.1|3.9e-16681.09PREDICTED: ervatamin-B-like [Cucumis melo][more]
gi|778722411|ref|XP_011658479.1|1.8e-16378.92PREDICTED: ervatamin-B-like [Cucumis sativus][more]
gi|700210691|gb|KGN65787.1|8.7e-15880.40hypothetical protein Csa_1G528520 [Cucumis sativus][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000169Pept_cys_AS
IPR000668Peptidase_C1A_C
IPR013128Peptidase_C1A
IPR013201Prot_inhib_I29
Vocabulary: Biological Process
TermDefinition
GO:0006508proteolysis
Vocabulary: Molecular Function
TermDefinition
GO:0008234cysteine-type peptidase activity
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0005575 cellular_component
molecular_function GO:0008234 cysteine-type peptidase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI04G03270.1CSPI04G03270.1mRNA


Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000169Cysteine peptidase, cysteine active sitePROSITEPS00139THIOL_PROTEASE_CYScoord: 149..160
scor
IPR000668Peptidase C1A, papain C-terminalPRINTSPR00705PAPAINcoord: 292..302
score: 3.3E-9coord: 149..164
score: 3.3E-9coord: 307..313
score: 3.
IPR000668Peptidase C1A, papain C-terminalPFAMPF00112Peptidase_C1coord: 131..346
score: 2.5
IPR000668Peptidase C1A, papain C-terminalSMARTSM00645pept_c1coord: 131..347
score: 1.3
IPR013128Peptidase C1APANTHERPTHR12411CYSTEINE PROTEASE FAMILY C1-RELATEDcoord: 1..348
score: 8.8E
IPR013201Cathepsin propeptide inhibitor domain (I29)PFAMPF08246Inhibitor_I29coord: 41..96
score: 8.
IPR013201Cathepsin propeptide inhibitor domain (I29)SMARTSM00848Inhibitor_I29_2coord: 41..96
score: 1.
NoneNo IPR availableGENE3DG3DSA:3.90.70.10coord: 8..347
score: 7.1E
NoneNo IPR availablePANTHERPTHR12411:SF346KDEL-TAILED CYSTEINE ENDOPEPTIDASE CEP1-RELATEDcoord: 1..348
score: 8.8E
NoneNo IPR availableunknownSSF54001Cysteine proteinasescoord: 37..347
score: 6.1