CSPI04G02860 (gene) Wild cucumber (PI 183967)

NameCSPI04G02860
Typegene
OrganismCucumis sativus (Wild cucumber (PI 183967))
DescriptionEukaryotic translation initiation factor eIF2A family protein
LocationChr4 : 1764749 .. 1769267 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTTCATATGTTAACGATTAACCTAATAAAAGAAATTTGTTTGGGGTTTTACCGGTCTTTCTTCTTCACCTTCTCCGGCATCGGAAGGAGCGTACTCTATCTGAAGTCGGCCCTAATTTCATCATCACACTCAACCTCCATTCAATCTTCCTTCTTCTGTTACTTGTACCAATTTCATTGATCTTCAATCAATTCGTCTCCGACTCCCATGGCCACCTCTCAACATCCACCACCTTTACAGATTCTTGGTAAGTTTTCTTCTAGCTCATTCATTTTTCTCTCTCTTTCAATTTTCTCATCCTTAAACTTCCTCCCAGTTCGAGGATCAGATGGTTTTTCAATTTGGAATGGACCCCCATTCATCGACAACCAACCCACCATCAACAAACTCGACAAGGTTGCTTGCACCAGTACGAAATTCAGTGAAGATGGATCCAAATTGCTGGTTACGAAGTCTGAAACTTTGAGCATTTACGAATGCCGTGGTTTTGGAGGGATCAAGTCTTTTGAAGTACCCAATTTGCTTGCGGCTGCTTTGTCACCATGTGGGACTTATCTCCAGACATTTCAGAAATCTTCAACTCCTCAAGAAAAGAATGTTGTTCTCTGGAAGGTTGACACTGGTGATCTTGTGTACTCTCAATTTCAGAAGAATATGACCAAAGTGTCATGGTGAGCGGTGTCTTGTATTTATGACGATTTATAGGCTTGGTCTTTTTCGTTTGTAGCTTATTATGTACTAGAGAAATTGGTTTTATATAACGTTTAGATTCCTTAGTGGGTCATTGAACCTTTGTGTTTTTTAAGATATTTATAAACTTCAAACACACTTCATCCTCCAGGCCTGCAATTCGATTCAGCTCTGATGAAACTGTTGCCTGTCGGATGGCTACTAATGAGATACAGTTTTTTGATGCTAAAGATTTCTCTAAGGGAGTTATTCATCGGCTTAGAGTTCCAGGAGTCACTGCAATTGAACTCTCAAGGACACCTGGAACCCACATTGCAGCCTTTGTTCCCGAGTCCAAGGTTGGTCTGTTAGACGAGGTTTGCTCATGGAGATTCATAAACTCCACCATTTTAGTGGTTTGTTGTTTTGTATTTATGCAGGGGGTTCCTGCCAGTGTCCAAATCTTTGCTTGTGGAGATTCACAAACTCAACCGATTGCTCGACGAAGTTTTTTCCGTTGTTCAACGGTACAACTAAACTGGAACAATGGTTCTACTGGGCTTCTAATTGTTGTGCAAGCAGATGTAGATAAAACCAACCAGAGTTATTATGGTGAAACAAAGTTAAACTACTTGACAACCGATGGTGTTCATGAAGGACTTGTACCACTTCGTAAGCACTAAATTCCTTTTCTTCAAATATTTGTTGCAGTACGTTCCAACACCCTAGCCAGCTATTATTGACATTAAAGTTTCCAGAGAGAAAGAACTGATTGTTAAGGACCTTGGCAGGTAAAGAAGGGCCCGTCCATGATGTGCAGTGGTCACAGAGTGGTTCGGAGTTTGCAGTAGTATATGGGTGTATCCTTATTATTGTACCATATTGCGGAAGGTTTATCTTATCTTTCACAATTAGATTGCACATTCAAATTTAACAATCTGTATTAATAAAGAGGATAAGCATATAGTACATGATGATGGTGTACATTATAGACTAGGAGATCATTTGCCAATAGCTCCCGGCATACTATGGCCAGACTTCTTCGATTGTAGCTCCATGCTTACTATGATTAAACATTTTCACGTGCTTTACATTGTTTTTTAAGTGGAATGGCTAAATATAATGTGAAACTAATCCAACCAAATGCTAGATTAATGAATTTGGTTGTTTTCATATTCCGTTACTATATAATATATACGTTACATGAATGTACTGGATGTGTACTCTGCTTGATGGTTCCTTCAACACTATAAATCTATGCATAACTCCTTTTAAATCCAATTATGGGTTTGTAAAGATCTTCAACATTTTTCCAAGAGCAAAAGAATATGTACAAAGAAAAGTTAATGTTCGGTAGAACATCTTGTTGACTGTTGTACCTTGGGATAATTCAATAATTAATAATTTTTAGAGGCATATACTCTCTCTTGAGCTCGGATGTTTAAATCTCCACCTCAATAATGCTGTACTCCAGCAATTATGATAAATGACATCTAAATGTCCTTATTCTTGTACTTTGCTTCTGCTAGCTGTGTTTTCTCTTTGCCTTGCACTTGTTCCTTGACAAAAAAAAAGTTATGCCTGCAAGAGCAACGGTGTTTGACAAGAAGTGCAAGCCTCTTCTTGAGCTTGGAGAAGGTCCCTACAACACTGTACGATGGAACCCGAAGGGGAAATGTATGTTTTCTGCTTTCATCTTTGGGATATCTACCTATGCTTTCTCTTCTCCATTTTCTTTAGCTTTTTCCCCATCTTGTTTTTCTTTTCTCTTCTTTTCTTTTCTTTTCTTCTCCTCTTTTCTTTCTTTCAAGGGAGGAACTACTATGGTGTCATTAGTGTTATTTTGTGACCTTAATTCTCTGGCCTGCAGTTCTATGTTTGGCTGGCTTCGGTAATCTGCCTGGTGACATGGTATTCTCTCTGTTTCATATATTTATTATGTGTCGTGTTCTCTGTTTTGCATAAAATATGGGCAGAAGTTTCTTGTTATTGTTTATGGCATGTTTCTCTTCAGAGTACTAACTTGATAAATTTCAAGAACTTTCCAAATTCGTTAAACATTGTACATCTGTATAAGCAATGGTCCAACCCCTATTATATTGATTATCACCGGTCAAGACTTGCGCTCATTATAGTATTATGCTCTTGCCTTTTCTTGTCTTCCCATGATTGAAGGAATGATATTCATATTTTATTTTCACATTTACAATTTCTTCTGCTTGCAATCATCTTTAACAGGCATTTTGGGACTACAAGGAGAAAAAACAACTTGGAAGAACAAAAGCTGAATGCTCCGTTACTAGCGAATGGTCCCCAGATGGGCAGTTTTTCATGACTGCCACAACGGCTCCAAGACTTCAAGTTGACAATGGGTAAACACTTTTGTACTATGTACAAAGATGGTGCTTGTAACCTAAATAATGTGAAAAGAGACTAAAAACTAATGTTGCTCAAGGCTTAAGGCCTAAGGGGGTATCAAGTTGTGTTATGTACTTTAAGCACGTTCATTTTTTCTGATGCATTCAAATTTCCACCTGTATAATATTCTTGGTTTCCCAATCGGCGGACAGGAGGATTTTGGTCAGCTATAAATAAGTTGATATCATTGTTTTCCTTGTATAGGATAAAAATTTTCCATTACAATGGAGCTTTATACTTCAAGAAAATGTTTGACAAGTTGTTCCAGGTAAGAGCTGTTCACCTTTCATTTTAAAAGATGTTTAACATGACTACTTCTCGCTCTTTTCCTCATGCAAACATTTTGTGCAGGCCGATTGGAAGCCAGAGTCTTCAGATAAGTTTGGTGAAATCGATCTACTAGTTAAGTCTGTAGAATCGCTAAATGTTGATCAAAAGAAAACACAAGGTTCCATCACATAACTGTTATCTTATGTCTATATGCTTCTGTATTTATCCAGTTTAATTGAGCAATTAGATTGTCCTAAATCCAGGTCAAGGGTCAAAAGTCTCACAAACATCGCAGAAAACCGCACCTTCGAACCCACCTGTTCAGAAGCCTGCGGCTTATAGGCCTCCACATGCGAAAAATGCTGCTGCTATTCAGGCAGAGGTATTGATTTTCAAGTTCCTCTCCCTTTCCATCTAAGTGAGGCCATTATGGTTTTTATATTTGAAGATTTCCAATTCAAGTTCTTATAAGCTAAGCTTTTTTGTAATGCAGTTGCTTGGAGAAGGCCCTAAAGAGTAAGTCCATTAATTATAGGCGTGTCATTTATCGTACTCTTAACAGGCTAAATTCTGGGATTAACCGAATATATGAGTTAAATGCAGAACACTGAGTAAGAACGCCATGCGGAACAAGAAGAAAAGGGAGAAACAAAAGGAGAAGAAGGCAGGGGAAGCTGCTTCATCAGCCAATGATGTGTGAGGTATGCAGTATACAACATAGAATTCAGATGTTTCCATGTTCGCTTCTAGCATTTGCTCCAGGAGCTTTTGAATTTGAAGCTGAGAGCTTTCTCAACAACGGATTCAAGTGAAATATTAACTGCCAAGGGAGACAATAGGTAGGTTCAATCCTTCAAGAATTTTGAGAAAAATGTTAAATGTAACACAAGGCTTAAGAGTCTCTTGAGTACCAAGTTATTATTGGTCTTATCTTTATGGTTTTATAAACCTTAAAAAGGTGAACCATCCTTAGCTGCAGTTATCATGTTCTTGGATTATTTTCATTATGATTCGAAGTTTCAACGCCAAACTGACCAAAATCAGATCAAGCCAATATGATTGGAACCAAGAAAATTAAACAAATTGAACCAGCTATCATTTGCTGTATGCTTCAGAAATTGAGCTGAATACATGTAGTATATATAAACCAATTGAATCAAG

mRNA sequence

ATGGCCACCTCTCAACATCCACCACCTTTACAGATTCTTGTTCGAGGATCAGATGGTTTTTCAATTTGGAATGGACCCCCATTCATCGACAACCAACCCACCATCAACAAACTCGACAAGGTTGCTTGCACCAGTACGAAATTCAGTGAAGATGGATCCAAATTGCTGGTTACGAAGTCTGAAACTTTGAGCATTTACGAATGCCGTGGTTTTGGAGGGATCAAGTCTTTTGAAGTACCCAATTTGCTTGCGGCTGCTTTGTCACCATGTGGGACTTATCTCCAGACATTTCAGAAATCTTCAACTCCTCAAGAAAAGAATGTTGTTCTCTGGAAGGTTGACACTGGTGATCTTGTGTACTCTCAATTTCAGAAGAATATGACCAAAGTGTCATGGCCTGCAATTCGATTCAGCTCTGATGAAACTGTTGCCTGTCGGATGGCTACTAATGAGATACAGTTTTTTGATGCTAAAGATTTCTCTAAGGGAGTTATTCATCGGCTTAGAGTTCCAGGAGTCACTGCAATTGAACTCTCAAGGACACCTGGAACCCACATTGCAGCCTTTGTTCCCGAGTCCAAGGGGGTTCCTGCCAGTGTCCAAATCTTTGCTTGTGGAGATTCACAAACTCAACCGATTGCTCGACGAAGTTTTTTCCGTTGTTCAACGGTACAACTAAACTGGAACAATGGTTCTACTGGGCTTCTAATTGTTGTGCAAGCAGATGTAGATAAAACCAACCAGAGTTATTATGGTGAAACAAAGTTAAACTACTTGACAACCGATGGTGTTCATGAAGGACTTGTACCACTTCGTAAAGAAGGGCCCGTCCATGATGTGCAGTGGTCACAGAGTGGTTCGGAGTTTGCAGTAGTATATGGGTTTATGCCTGCAAGAGCAACGGTGTTTGACAAGAAGTGCAAGCCTCTTCTTGAGCTTGGAGAAGGTCCCTACAACACTGTACGATGGAACCCGAAGGGGAAATTTCTATGTTTGGCTGGCTTCGGTAATCTGCCTGGTGACATGGCATTTTGGGACTACAAGGAGAAAAAACAACTTGGAAGAACAAAAGCTGAATGCTCCGTTACTAGCGAATGGTCCCCAGATGGGCAGTTTTTCATGACTGCCACAACGGCTCCAAGACTTCAAGTTGACAATGGGATAAAAATTTTCCATTACAATGGAGCTTTATACTTCAAGAAAATGTTTGACAAGTTGTTCCAGGCCGATTGGAAGCCAGAGTCTTCAGATAAGTTTGGTGAAATCGATCTACTAGTTAAGTCTGTAGAATCGCTAAATGTTGATCAAAAGAAAACACAAGGTCAAGGGTCAAAAGTCTCACAAACATCGCAGAAAACCGCACCTTCGAACCCACCTGTTCAGAAGCCTGCGGCTTATAGGCCTCCACATGCGAAAAATGCTGCTGCTATTCAGGCAGAGTTGCTTGGAGAAGGCCCTAAAGAAACACTGAGTAAGAACGCCATGCGGAACAAGAAGAAAAGGGAGAAACAAAAGGAGAAGAAGGCAGGGGAAGCTGCTTCATCAGCCAATGATGTGTGA

Coding sequence (CDS)

ATGGCCACCTCTCAACATCCACCACCTTTACAGATTCTTGTTCGAGGATCAGATGGTTTTTCAATTTGGAATGGACCCCCATTCATCGACAACCAACCCACCATCAACAAACTCGACAAGGTTGCTTGCACCAGTACGAAATTCAGTGAAGATGGATCCAAATTGCTGGTTACGAAGTCTGAAACTTTGAGCATTTACGAATGCCGTGGTTTTGGAGGGATCAAGTCTTTTGAAGTACCCAATTTGCTTGCGGCTGCTTTGTCACCATGTGGGACTTATCTCCAGACATTTCAGAAATCTTCAACTCCTCAAGAAAAGAATGTTGTTCTCTGGAAGGTTGACACTGGTGATCTTGTGTACTCTCAATTTCAGAAGAATATGACCAAAGTGTCATGGCCTGCAATTCGATTCAGCTCTGATGAAACTGTTGCCTGTCGGATGGCTACTAATGAGATACAGTTTTTTGATGCTAAAGATTTCTCTAAGGGAGTTATTCATCGGCTTAGAGTTCCAGGAGTCACTGCAATTGAACTCTCAAGGACACCTGGAACCCACATTGCAGCCTTTGTTCCCGAGTCCAAGGGGGTTCCTGCCAGTGTCCAAATCTTTGCTTGTGGAGATTCACAAACTCAACCGATTGCTCGACGAAGTTTTTTCCGTTGTTCAACGGTACAACTAAACTGGAACAATGGTTCTACTGGGCTTCTAATTGTTGTGCAAGCAGATGTAGATAAAACCAACCAGAGTTATTATGGTGAAACAAAGTTAAACTACTTGACAACCGATGGTGTTCATGAAGGACTTGTACCACTTCGTAAAGAAGGGCCCGTCCATGATGTGCAGTGGTCACAGAGTGGTTCGGAGTTTGCAGTAGTATATGGGTTTATGCCTGCAAGAGCAACGGTGTTTGACAAGAAGTGCAAGCCTCTTCTTGAGCTTGGAGAAGGTCCCTACAACACTGTACGATGGAACCCGAAGGGGAAATTTCTATGTTTGGCTGGCTTCGGTAATCTGCCTGGTGACATGGCATTTTGGGACTACAAGGAGAAAAAACAACTTGGAAGAACAAAAGCTGAATGCTCCGTTACTAGCGAATGGTCCCCAGATGGGCAGTTTTTCATGACTGCCACAACGGCTCCAAGACTTCAAGTTGACAATGGGATAAAAATTTTCCATTACAATGGAGCTTTATACTTCAAGAAAATGTTTGACAAGTTGTTCCAGGCCGATTGGAAGCCAGAGTCTTCAGATAAGTTTGGTGAAATCGATCTACTAGTTAAGTCTGTAGAATCGCTAAATGTTGATCAAAAGAAAACACAAGGTCAAGGGTCAAAAGTCTCACAAACATCGCAGAAAACCGCACCTTCGAACCCACCTGTTCAGAAGCCTGCGGCTTATAGGCCTCCACATGCGAAAAATGCTGCTGCTATTCAGGCAGAGTTGCTTGGAGAAGGCCCTAAAGAAACACTGAGTAAGAACGCCATGCGGAACAAGAAGAAAAGGGAGAAACAAAAGGAGAAGAAGGCAGGGGAAGCTGCTTCATCAGCCAATGATGTGTGA
BLAST of CSPI04G02860 vs. Swiss-Prot
Match: EIF2A_CHICK (Eukaryotic translation initiation factor 2A OS=Gallus gallus GN=EIF2A PE=2 SV=1)

HSP 1 Score: 290.8 bits (743), Expect = 2.9e-77
Identity = 185/537 (34.45%), Postives = 272/537 (50.65%), Query Frame = 1

Query: 7   PPPLQILVRGSDGFSIWNGPPFIDNQPTINKLDKVACTSTKFSEDGSKLLVTKSETLSIY 66
           PP   + VRGS+G  + NGPP         +     C +  FS+DGS       E ++I 
Sbjct: 3   PPAPLLAVRGSEGLFMVNGPPSFTESAVFQRDSGRNCKAVAFSKDGSLFAWCNGEKVNIV 62

Query: 67  ECRGFGGIKSFEVPNLLAAALSPCGTYLQTFQ-----KSSTPQEKNVVLWKVDTGDLVYS 126
                G ++SF++P ++    SP    L T+Q     K  T    N+ L+ V TG  + S
Sbjct: 63  NVTSAGLLRSFDLPKVVCLEFSPKNNILATWQAYSAAKDGTAGAPNLQLYDVKTGKCLKS 122

Query: 127 QFQKNMTKVSWPAIRFSSDETVACRMATNEIQFFDAKDFSKGVIHRLRVPGVTAIELSRT 186
             QK M   +W    ++ DE++  R   NE+ FF+  +F+  + ++L +  V    LS  
Sbjct: 123 FIQKKMQ--NWCPC-WADDESICARNVNNEVHFFENNNFNT-IANKLHLQKVNDFVLS-- 182

Query: 187 PG---THIAAFVPESKGVPASVQIFACGD--SQTQPIARRSFFRCSTVQLNWNNGSTGLL 246
           PG   T +A +VP SKG P+ V+++   +       +A +SFF+   V + WN  +T +L
Sbjct: 183 PGAQPTKVAVYVPGSKGAPSFVRLYQYPNFGGPQSALANKSFFKADKVTMLWNKKATAVL 242

Query: 247 IVVQADVDKTNQSYYGETKLNYLTTDGVHEGLVPLRKEGPVHDVQWSQSGSEFAVVYGFM 306
           ++   DVDKT  SYYGE  L+Y+ T+G    +V L K GP++DV W+ +  EF  VYGFM
Sbjct: 243 VIASTDVDKTGASYYGEQTLHYIATNG-ESAIVQLPKNGPIYDVVWNPNSVEFCAVYGFM 302

Query: 307 PARATVFDKKCKPLLELGEGPYNTVRWNPKGKFLCLAGFGNLPGDMAFWDYKEKKQLGRT 366
           PA+ATVF+ KC P+ + G GP N   ++P G  L LAGFGNL G M  WD K  K + + 
Sbjct: 303 PAKATVFNLKCDPVFDFGTGPRNAAYYSPHGHILVLAGFGNLRGQMEVWDVKNYKLISKP 362

Query: 367 KAECSVTSEWSPDGQFFMTATTAPRLQVDNGIKIFHYNGAL---YFKKMFDKLFQADWKP 426
            A  S    W PDG+  +TAT APRL+V NG KI+HY G++   Y     ++++Q  W+P
Sbjct: 363 VASDSTYFAWCPDGEHIVTATCAPRLRVSNGYKIWHYTGSVLHNYEVPSNEEMWQVSWQP 422

Query: 427 ESSDKFGEIDLLVKSVESLNVDQKKTQGQGSKVSQTSQKTAPSNPPVQKPA-AYRPPHAK 486
                F      VK+V+                 Q      PS  P  KPA AYRPP  +
Sbjct: 423 FLDGVFP-----VKAVK----------------YQAVPSELPSAEP--KPAQAYRPPALR 482

Query: 487 NAAAIQAELLGEGP-----------KETLSKNAMRNKKKREKQKEKKAGEAASSAND 519
           N     ++L  + P           ++ LSK A++N+KK E +K  K    A  + +
Sbjct: 483 NKPVTSSKLHEDEPPQNMKPQSGSSEKPLSKTALKNQKKHEPKKAAKQEAKADCSQE 509

BLAST of CSPI04G02860 vs. Swiss-Prot
Match: EIF2A_MOUSE (Eukaryotic translation initiation factor 2A OS=Mus musculus GN=Eif2a PE=1 SV=2)

HSP 1 Score: 290.4 bits (742), Expect = 3.8e-77
Identity = 184/533 (34.52%), Postives = 267/533 (50.09%), Query Frame = 1

Query: 12  ILVRGSDGFSIWNGPPFIDNQPTINKLDKVACTSTKFSEDGSKLLVTKSETLSIYECRGF 71
           + VRGS+G  + NGPP       + +     C    FS+DG+    +  E ++I      
Sbjct: 8   LTVRGSEGLYMVNGPPHFTESTVLPRESGRNCKVYTFSKDGTLFAWSNGEKVNIINVANK 67

Query: 72  GGIKSFEVPNLLAAALSPCGTYLQTFQ-----KSSTPQEKNVVLWKVDTGDLVYSQFQKN 131
           G + SF++P  +    SP  T L T+Q     K  T    N+ L+ + TG  + S  QK 
Sbjct: 68  GLLHSFDLPKAVCLEFSPNNTVLATWQPYTTSKDGTAGTPNLQLYDMKTGACLKSFIQKK 127

Query: 132 MTK--VSWPAIRFSSDETVACRMATNEIQFFDAKDFSKGVIHRLRVPGVTAIELSRTPGT 191
           M     SW     S DE +  R   NE+ FF+  +F+  + ++L +  V    LS  PGT
Sbjct: 128 MQNWCPSW-----SDDEIICARNVNNEVHFFENNNFNT-IANKLHLQKVNDFNLS--PGT 187

Query: 192 H---IAAFVPESKGVPASVQIFACGD--SQTQPIARRSFFRCSTVQLNWNNGSTGLLIVV 251
               +A +VP SKG P+ V+++   +       +A +SFF+   V + WN  +T +L++ 
Sbjct: 188 QPYKVAVYVPGSKGAPSFVRLYQYPNFAGPQAALANKSFFKADKVTMLWNKKATAVLVIA 247

Query: 252 QADVDKTNQSYYGETKLNYLTTDGVHEGLVPLRKEGPVHDVQWSQSGSEFAVVYGFMPAR 311
             +VDKT  SYYGE  L+Y+ T+G    +V L K GP++DV W+ S +EF  VYGFMPA+
Sbjct: 248 STEVDKTGASYYGEQTLHYIATNG-ESAVVQLPKNGPIYDVVWNSSSTEFCAVYGFMPAK 307

Query: 312 ATVFDKKCKPLLELGEGPYNTVRWNPKGKFLCLAGFGNLPGDMAFWDYKEKKQLGRTKAE 371
           ATVF+ KC P+ + G GP N   ++P G  L LAGFGNL G M  WD K  K + +  A 
Sbjct: 308 ATVFNLKCDPVFDFGTGPRNAAFYSPHGHILVLAGFGNLRGQMEVWDVKNYKLISKPVAS 367

Query: 372 CSVTSEWSPDGQFFMTATTAPRLQVDNGIKIFHYNGALYFK---KMFDKLFQADWKPESS 431
            S    W PDG+  +TAT APRL+V+NG KI+HY G+L  K       +L+Q  W+P   
Sbjct: 368 DSTYFAWCPDGEHILTATCAPRLRVNNGYKIWHYTGSLLHKYDVPSNGELWQVSWQPFLD 427

Query: 432 DKFGEIDLLVKSVESLNVDQKKTQGQGSKVSQTSQKTAPSNPPVQKP---AAYRPPHAKN 491
             F    +  ++V                         PS  P ++P    AYRPP  +N
Sbjct: 428 GIFPAKTIKYQAV-------------------------PSEVPSEEPKVATAYRPPALRN 487

Query: 492 AAAIQAELLGEGPKET----------LSKNAMRNKKKREKQKEKKAGEAASSA 517
                ++L  E P +           LSK A++N++K E +K  K  EA S A
Sbjct: 488 KPVTNSKLHEEEPPQNMKPHPGSDKPLSKTALKNQRKHEAKKAAKQ-EARSDA 505

BLAST of CSPI04G02860 vs. Swiss-Prot
Match: EIF2A_DANRE (Eukaryotic translation initiation factor 2A OS=Danio rerio GN=eif2a PE=2 SV=1)

HSP 1 Score: 289.7 bits (740), Expect = 6.5e-77
Identity = 185/540 (34.26%), Postives = 282/540 (52.22%), Query Frame = 1

Query: 7   PPPLQILVRGSDGFSIWNGPPFIDNQPTINKLDKVACTSTKFSEDGSKLLVTKSETLSIY 66
           PP   + VRGSDG  + +GPP         + D+     T FS DG+         +++ 
Sbjct: 3   PPTPHLAVRGSDGTLLLHGPPNCQESSAFQRDDRQGRYMT-FSNDGTLFAWCNGSQVTVL 62

Query: 67  ECRGFGGIKSFEVPNLLAAALSPCGTYLQTFQKSSTPQ-----EKNVVLWKVDTGDLVYS 126
           +      +KSF++P   A   SP    L T+Q+ +  Q     E N+ LW + TG  + +
Sbjct: 63  KVPSGDLVKSFDLPKTTALEFSPLNKVLATWQQYTKTQDNPQGEANLQLWDLQTGACMKA 122

Query: 127 QFQKNMTKVSWPAIRFSSDETVACRMATNEIQFFDAKDFSKGVIHRLRVPGVTAIELSRT 186
            +QK MT   W    ++ DE+++ R   NE+ FF+  +F   + ++L +  V+   LS  
Sbjct: 123 FYQKKMT--GW-CPSWADDESISVRNVNNELHFFENNNFET-IANKLHLQKVSEFALS-- 182

Query: 187 PGTH---IAAFVPESKGVPASVQIFACGD--SQTQPIARRSFFRCSTVQLNWNNGSTGLL 246
           PG+    +A +VP SKG P+ V+++   +    T  +A +SFF+   V + WN  +T +L
Sbjct: 183 PGSQPSKVAVYVPGSKGAPSFVRLYQYPNFGGPTCALANKSFFKADKVNMLWNKKATAVL 242

Query: 247 IVVQADVDKTNQSYYGETKLNYLTTDGVHEGLVPLRKEGPVHDVQWSQSGSEFAVVYGFM 306
           +    +VDKT  SYYGE  L+Y+ T+G    +V L K GP++DV WS + +EF VVYGFM
Sbjct: 243 VTASTEVDKTGASYYGEQTLHYVATNG-ESAVVQLPKNGPIYDVSWSPNSTEFCVVYGFM 302

Query: 307 PARATVFDKKCKPLLELGEGPYNTVRWNPKGKFLCLAGFGNLPGDMAFWDYKEKKQLGRT 366
           PA+ATVF+ KC+ + + G GP N   ++P+G  L LAGFGNL G M  WD K+ KQ+ + 
Sbjct: 303 PAKATVFNNKCESVFDFGTGPRNAAFYSPQGHILVLAGFGNLRGQMEVWDVKKYKQVSKP 362

Query: 367 KAECSVTSEWSPDGQFFMTATTAPRLQVDNGIKIFHYNGALYFK---KMFDKLFQADWKP 426
           +AE +    W PDG+  +TAT +PRL+  NG KI+HY G + +K       +L++  W+P
Sbjct: 363 QAEDTTHFSWCPDGEHIVTATCSPRLRASNGYKIWHYTGTVLYKHETPSGKELWEVLWQP 422

Query: 427 ESSDKFGEIDLLVKSVESLNVDQKKTQGQGSKVSQTSQKTAPSNPPVQKPAAYRPPHAKN 486
             +  F E     ++V        K Q   S++  T  K A          AYRPP  +N
Sbjct: 423 FPAGVFPE-----RAV--------KYQALPSELGSTEAKPA---------QAYRPPALRN 482

Query: 487 -AAAIQAELLGEGPKETL----------SKNAMRNKKKREKQK----EKKAGEAASSAND 519
                 ++L  E P + +          SK A++N+K+RE +K    E K  EA   A D
Sbjct: 483 KPQTASSKLHEEEPPQNMKPGAAGEKQPSKAALKNQKRREAKKAAKQENKPDEAPPPAAD 512

BLAST of CSPI04G02860 vs. Swiss-Prot
Match: EIF2A_XENLA (Eukaryotic translation initiation factor 2A OS=Xenopus laevis GN=eif2a PE=2 SV=2)

HSP 1 Score: 287.7 bits (735), Expect = 2.5e-76
Identity = 183/540 (33.89%), Postives = 278/540 (51.48%), Query Frame = 1

Query: 8   PPLQIL-VRGSDGFSIWNGPPFIDNQPTINKLDKVACTSTKFSEDGSKLLVTKSETLSIY 67
           PP+ +L VRGSDG  +  GPP   +  T  +     C  ++F++DG        E ++I 
Sbjct: 3   PPMPLLSVRGSDGIQLVLGPPKFTDSETFQRDPSKNCKVSRFTKDGKLFGWCNGEVINIV 62

Query: 68  ECRGFGGIKSFEVPNLLAAALSPCGTYLQTFQ-----KSSTPQEKNVVLWKVDTGDL--V 127
            C     + + ++P +++   SP  T L T+Q     K  T    N+ L+ + +G +  V
Sbjct: 63  NCSDSKLLHTLDLPKVVSLEFSPKNTILATWQTYTTGKDGTAGTPNLQLYDLKSGKISNV 122

Query: 128 YSQFQKNMTKVSWPAIRFSSDETVACRMATNEIQFFDAKDFSKGVIHRLRVPGVTAIELS 187
            S  QK M   +W    +S DE +  R   NE+ FF+  +F   + ++L +  V+  ELS
Sbjct: 123 KSFIQKKME--NWSP-HWSDDENICARNVNNEVHFFENNNFDT-IANKLHLQKVSDFELS 182

Query: 188 RTPGTH---IAAFVPESKGVPASVQIFACGD--SQTQPIARRSFFRCSTVQLNWNNGSTG 247
             PG     +A +VP SKG P+ V+++   +       +A +SFF+   V + WN+ +T 
Sbjct: 183 --PGEQPCKVAVYVPGSKGAPSFVRLYQYPNFSGPNSALANKSFFKADRVAMLWNSKATA 242

Query: 248 LLIVVQADVDKTNQSYYGETKLNYLTTDGVHEGLVPLRKEGPVHDVQWSQSGSEFAVVYG 307
           +L+    DVDKT  SYYGE  L+Y+  +G    +V L K GP++DV W+++ +EF VVYG
Sbjct: 243 VLVTASTDVDKTGASYYGEQTLHYIAVNG-ESAVVQLPKNGPIYDVTWNKNATEFCVVYG 302

Query: 308 FMPARATVFDKKCKPLLELGEGPYNTVRWNPKGKFLCLAGFGNLPGDMAFWDYKEKKQLG 367
           FMPA+ATVF+ KC P+ + G GP N   ++P+G  L LAGFGNL G M  WD K+ K + 
Sbjct: 303 FMPAKATVFNLKCDPIFDFGTGPRNAAFYSPQGHILVLAGFGNLRGQMEVWDVKKYKLIS 362

Query: 368 RTKAECSVTSEWSPDGQFFMTATTAPRLQVDNGIKIFHYNGALYFK---KMFDKLFQADW 427
           +  A  +    W P+G+  +TAT +PRL+V NG KI+HY G L  K       +L+Q  W
Sbjct: 363 KPTASDATFFSWCPNGEHIITATCSPRLRVGNGYKIWHYTGTLLHKYDVPANSELWQVSW 422

Query: 428 KPESSDKFGEIDLLVKSVESLNVDQKKTQGQGSKVSQTSQKTAPSNPPVQ--KPA-AYRP 487
           +P     F    ++ ++V                         P + P Q  KPA AYRP
Sbjct: 423 QPFPDGVFPAKAIVYQAV-------------------------PGDLPTQESKPAEAYRP 482

Query: 488 PHAKNAAAIQAELLGEGPKET---------LSKNAMRNKKKREKQK----EKKAGEAASS 516
           P  +N      +L  + P ++         +SK A++N+KKRE +K    E K  E A S
Sbjct: 483 PALRNKPVSSYKLHEDEPPQSMMPQSTEKPMSKTALKNQKKREAKKAAKQESKMDEPAES 510

BLAST of CSPI04G02860 vs. Swiss-Prot
Match: EIF2A_HUMAN (Eukaryotic translation initiation factor 2A OS=Homo sapiens GN=EIF2A PE=1 SV=3)

HSP 1 Score: 285.4 bits (729), Expect = 1.2e-75
Identity = 178/536 (33.21%), Postives = 268/536 (50.00%), Query Frame = 1

Query: 12  ILVRGSDGFSIWNGPPFIDNQPTINKLDKVACTSTKFSEDGSKLLVTKSETLSIYECRGF 71
           + VRGS+G  + NGPP         +     C    FS+DG+       E ++I      
Sbjct: 8   LTVRGSEGLYMVNGPPHFTESTVFPRESGKNCKVCIFSKDGTLFAWGNGEKVNIISVTNK 67

Query: 72  GGIKSFEVPNLLAAALSPCGTYLQTFQKSSTPQE-----KNVVLWKVDTGDLVYSQFQKN 131
           G + SF++   +    SP  T L T+Q  +T ++      N+ L+ V TG  + S  QK 
Sbjct: 68  GLLHSFDLLKAVCLEFSPKNTVLATWQPYTTSKDGTAGIPNLQLYDVKTGTCLKSFIQKK 127

Query: 132 MTK--VSWPAIRFSSDETVACRMATNEIQFFDAKDFSKGVIHRLRVPGVTAIELSRTPGT 191
           M     SW     S DET+  R   NE+ FF+  +F+  + ++L +  +    LS  P  
Sbjct: 128 MQNWCPSW-----SEDETLCARNVNNEVHFFENNNFNT-IANKLHLQKINDFVLSPGPQP 187

Query: 192 H-IAAFVPESKGVPASVQIFACGD--SQTQPIARRSFFRCSTVQLNWNNGSTGLLIVVQA 251
           + +A +VP SKG P+ V+++   +       +A +SFF+   V + WN  +T +L++   
Sbjct: 188 YKVAVYVPGSKGAPSFVRLYQYPNFAGPHAALANKSFFKADKVTMLWNKKATAVLVIAST 247

Query: 252 DVDKTNQSYYGETKLNYLTTDGVHEGLVPLRKEGPVHDVQWSQSGSEFAVVYGFMPARAT 311
           DVDKT  SYYGE  L+Y+ T+G    +V L K GP++DV W+ S +EF  VYGFMPA+AT
Sbjct: 248 DVDKTGASYYGEQTLHYIATNG-ESAVVQLPKNGPIYDVVWNSSSTEFCAVYGFMPAKAT 307

Query: 312 VFDKKCKPLLELGEGPYNTVRWNPKGKFLCLAGFGNLPGDMAFWDYKEKKQLGRTKAECS 371
           +F+ KC P+ + G GP N   ++P G  L LAGFGNL G M  WD K  K + +  A  S
Sbjct: 308 IFNLKCDPVFDFGTGPRNAAYYSPHGHILVLAGFGNLRGQMEVWDVKNYKLISKPVASDS 367

Query: 372 VTSEWSPDGQFFMTATTAPRLQVDNGIKIFHYNGALYFKKMFD-----KLFQADWKPESS 431
               W PDG+  +TAT APRL+V+NG KI+HY G++  K  +D     +L+Q  W+P   
Sbjct: 368 TYFAWCPDGEHILTATCAPRLRVNNGYKIWHYTGSILHK--YDVPSNAELWQVSWQPFLD 427

Query: 432 DKFGEIDLLVKSVESLNVDQKKTQGQGSKVSQTSQKTAPSNPPVQKP---AAYRPPHAKN 491
             F    +  ++V                         PS  P ++P    AYRPP  +N
Sbjct: 428 GIFPAKTITYQAV-------------------------PSEVPNEEPKVATAYRPPALRN 487

Query: 492 AAAIQAELLGEGPKET----------LSKNAMRNKKKREKQKEKKAGEAASSANDV 520
                ++L  E P +           LSK A++N++K E +K  K    +  + D+
Sbjct: 488 KPITNSKLHEEEPPQNMKPQSGNDKPLSKTALKNQRKHEAKKAAKQEARSDKSPDL 509

BLAST of CSPI04G02860 vs. TrEMBL
Match: A0A0A0KXB3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G011690 PE=4 SV=1)

HSP 1 Score: 1050.4 bits (2715), Expect = 7.0e-304
Identity = 516/519 (99.42%), Postives = 517/519 (99.61%), Query Frame = 1

Query: 1   MATSQHPPPLQILVRGSDGFSIWNGPPFIDNQPTINKLDKVACTSTKFSEDGSKLLVTKS 60
           MATSQHPPPLQILVRGSDGFSIWNGPPFIDNQPTINKLDKVACTSTKFSEDGSKLLVTKS
Sbjct: 1   MATSQHPPPLQILVRGSDGFSIWNGPPFIDNQPTINKLDKVACTSTKFSEDGSKLLVTKS 60

Query: 61  ETLSIYECRGFGGIKSFEVPNLLAAALSPCGTYLQTFQKSSTPQEKNVVLWKVDTGDLVY 120
           ETL+IYECRGFG IKSFEVPNLLAAALSPCGTYLQTFQKSSTPQEKNVVLWKVDTG LVY
Sbjct: 61  ETLTIYECRGFGEIKSFEVPNLLAAALSPCGTYLQTFQKSSTPQEKNVVLWKVDTGGLVY 120

Query: 121 SQFQKNMTKVSWPAIRFSSDETVACRMATNEIQFFDAKDFSKGVIHRLRVPGVTAIELSR 180
           SQFQKNMTKVSWPAIRFSSDETVACRMATNEIQFFDAKDFSKGVIHRLRVPGVTAIELSR
Sbjct: 121 SQFQKNMTKVSWPAIRFSSDETVACRMATNEIQFFDAKDFSKGVIHRLRVPGVTAIELSR 180

Query: 181 TPGTHIAAFVPESKGVPASVQIFACGDSQTQPIARRSFFRCSTVQLNWNNGSTGLLIVVQ 240
           TPGTHIAAFVPESKGVPASVQIFACGDSQTQPIARRSFFRCSTVQLNWNNGSTGLLIVVQ
Sbjct: 181 TPGTHIAAFVPESKGVPASVQIFACGDSQTQPIARRSFFRCSTVQLNWNNGSTGLLIVVQ 240

Query: 241 ADVDKTNQSYYGETKLNYLTTDGVHEGLVPLRKEGPVHDVQWSQSGSEFAVVYGFMPARA 300
           ADVDKTNQSYYGETKLNYLTTDGVHEGLVPLRKEGPVHDVQWSQSGSEFAVVYGFMPARA
Sbjct: 241 ADVDKTNQSYYGETKLNYLTTDGVHEGLVPLRKEGPVHDVQWSQSGSEFAVVYGFMPARA 300

Query: 301 TVFDKKCKPLLELGEGPYNTVRWNPKGKFLCLAGFGNLPGDMAFWDYKEKKQLGRTKAEC 360
           TVFDKKCKPLLELGEGPYNTVRWNPKGKFLCLAGFGNLPGDMAFWDYKEKKQLGRTKAEC
Sbjct: 301 TVFDKKCKPLLELGEGPYNTVRWNPKGKFLCLAGFGNLPGDMAFWDYKEKKQLGRTKAEC 360

Query: 361 SVTSEWSPDGQFFMTATTAPRLQVDNGIKIFHYNGALYFKKMFDKLFQADWKPESSDKFG 420
           SVTSEWSPDGQFFMTATTAPRLQVDNGIKIFHYNGALYFKKMFDKLFQADWKPESSDKFG
Sbjct: 361 SVTSEWSPDGQFFMTATTAPRLQVDNGIKIFHYNGALYFKKMFDKLFQADWKPESSDKFG 420

Query: 421 EIDLLVKSVESLNVDQKKTQGQGSKVSQTSQKTAPSNPPVQKPAAYRPPHAKNAAAIQAE 480
           EIDLLVKSVESLNVDQKKTQGQGSKVSQTSQKTAPSNPPVQKPAAYRPPHAKNAAAIQAE
Sbjct: 421 EIDLLVKSVESLNVDQKKTQGQGSKVSQTSQKTAPSNPPVQKPAAYRPPHAKNAAAIQAE 480

Query: 481 LLGEGPKETLSKNAMRNKKKREKQKEKKAGEAASSANDV 520
           LLGEGPKETLSKNAMRNKKKREKQKEKKAGEAASSANDV
Sbjct: 481 LLGEGPKETLSKNAMRNKKKREKQKEKKAGEAASSANDV 519

BLAST of CSPI04G02860 vs. TrEMBL
Match: M5XFE3_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa004316mg PE=4 SV=1)

HSP 1 Score: 825.1 bits (2130), Expect = 4.8e-236
Identity = 395/516 (76.55%), Postives = 454/516 (87.98%), Query Frame = 1

Query: 1   MATSQHPPPLQILVRGSDGFSIWNGPPFIDNQPTINKLDKVACTSTKFSEDGSKLLVTKS 60
           MA S+  PPL ILVRG +GFS+WNGPPF + QP I KL+ ++CTSTKFSEDGS+L+V KS
Sbjct: 1   MAASETAPPLDILVRGPEGFSLWNGPPFSNGQPGI-KLENISCTSTKFSEDGSRLMVMKS 60

Query: 61  ET-LSIYECRGFGGIKSFEVPNLLAAALSPCGTYLQTFQKSSTPQEKNVVLWKVDTGDLV 120
           ++ +SIY+C  +  I+SF++PN+ AAALSP GTY+QTFQKSSTPQEKNV+LW  +TGD V
Sbjct: 61  DSVISIYDCSSYREIRSFQIPNVAAAALSPRGTYVQTFQKSSTPQEKNVILWNTETGDPV 120

Query: 121 YSQFQKNMTKVSWPAIRFSSDETVACRMATNEIQFFDAKDFSKGVIHRLRVPGVTAIELS 180
           Y QFQKNMTK +WP+IRFSSDE VACR+ATNEIQF DA DFSK  I+RLRVPGV A ELS
Sbjct: 121 YQQFQKNMTKATWPSIRFSSDEAVACRLATNEIQFLDAGDFSKPFIYRLRVPGVAAFELS 180

Query: 181 RTPGTHIAAFVPESKGVPASVQIFACGDS-QTQPIARRSFFRCSTVQLNWNNGSTGLLIV 240
           + PG+H+AAFVPESKG+PASVQIF+C  + Q+QP+ARRSFFRCS+VQLNWNNGSTGLL+V
Sbjct: 181 KAPGSHVAAFVPESKGIPASVQIFSCAKAVQSQPLARRSFFRCSSVQLNWNNGSTGLLVV 240

Query: 241 VQADVDKTNQSYYGETKLNYLTTDGVHEGLVPLRKEGPVHDVQWSQSGSEFAVVYGFMPA 300
           VQ+DVDKTNQSYYGE+KLNYLTTDG HEGLVPLRKEGPVHDVQWS SGSEFAVVYGFMP+
Sbjct: 241 VQSDVDKTNQSYYGESKLNYLTTDGTHEGLVPLRKEGPVHDVQWSYSGSEFAVVYGFMPS 300

Query: 301 RATVFDKKCKPLLELGEGPYNTVRWNPKGKFLCLAGFGNLPGDMAFWDYKEKKQLGRTKA 360
           +ATVFDKKC PLLELG GPYNT+RWNPKGKFLCLAGFGNLPGDMAFWDY EKKQLG T+A
Sbjct: 301 KATVFDKKCNPLLELGTGPYNTIRWNPKGKFLCLAGFGNLPGDMAFWDYGEKKQLGTTRA 360

Query: 361 ECSVTSEWSPDGQFFMTATTAPRLQVDNGIKIFHYNGALYFKKMFDKLFQADWKPESSDK 420
           E SVTSEWSPDG +FMTATTAPRLQVDNGIKIFH+NG+L+FKKMFDKL+QADWKPES D+
Sbjct: 361 ELSVTSEWSPDGCYFMTATTAPRLQVDNGIKIFHHNGSLFFKKMFDKLYQADWKPESPDR 420

Query: 421 FGEIDLLVKSVESLNVDQKKTQGQGSKVSQTSQKTAPSNPPVQKPAAYRPPHAKNAAAIQ 480
           FG+I  LVKS+ SL V + K QG  SK+SQTS K + SNPP QKPAAYRPPHAK+AAA+Q
Sbjct: 421 FGDIAELVKSIGSLKVVETKPQGDRSKISQTSAKASASNPPAQKPAAYRPPHAKSAAAVQ 480

Query: 481 AELLGEGPKETLSKNAMRNKKKREKQKEKKAGEAAS 515
           A+L GE   ET+SKNA++NKKKREKQ+EKKA EA S
Sbjct: 481 AQLFGESSTETVSKNALKNKKKREKQREKKAAEATS 515

BLAST of CSPI04G02860 vs. TrEMBL
Match: A0A0G4AP04_9ROSI (Eukaryotic translation initiation factor eIF2A family protein OS=Hypseocharis bilobata PE=2 SV=1)

HSP 1 Score: 823.2 bits (2125), Expect = 1.8e-235
Identity = 393/518 (75.87%), Postives = 455/518 (87.84%), Query Frame = 1

Query: 1   MATSQHPPPLQILVRGSDGFSIWNGPPFIDNQPTINKLDKVACTSTKFSEDGSKLLVTKS 60
           MA +   P L+IL RG +GFS+WNGPPF + QP+I KL++V CT+ KFSEDGS+L+VTKS
Sbjct: 1   MAATDRSPTLEILARGPEGFSLWNGPPFSNGQPSI-KLERVPCTNAKFSEDGSRLMVTKS 60

Query: 61  ETL-SIYECRGFGGIKSFEVPNLLAAALSPCGTYLQTFQKSSTPQEKNVVLWKVDTGDLV 120
           +++ SIY+C     I++F+VPN+LAAALSP GTYLQTFQK STPQEKNVVLW  +TGD V
Sbjct: 61  DSVVSIYDCSTSKEIRTFQVPNILAAALSPRGTYLQTFQKFSTPQEKNVVLWNTETGDPV 120

Query: 121 YSQFQKNMTKVSWPAIRFSSDETVACRMATNEIQFFDAKDFSKGVIHRLRVPGVTAIELS 180
           Y QFQKNMTK +WP+IRFSSDE +ACR+ATNEIQFF+ +DFSKG ++RLRVPGV A+ELS
Sbjct: 121 YQQFQKNMTKTTWPSIRFSSDEAIACRLATNEIQFFNGRDFSKGTVNRLRVPGVAAVELS 180

Query: 181 RTPGTHIAAFVPESKGVPASVQIFACG-DSQTQPIARRSFFRCSTVQLNWNNGSTGLLIV 240
           + PG+H+AA+VPESKGVPASVQI+AC  D Q+QP+ARRSFFRCS+VQLNWN+GSTGLL++
Sbjct: 181 KAPGSHVAAYVPESKGVPASVQIYACEKDLQSQPVARRSFFRCSSVQLNWNHGSTGLLVL 240

Query: 241 VQADVDKTNQSYYGETKLNYLTTDGVHEGLVPLRKEGPVHDVQWSQSGSEFAVVYGFMPA 300
           VQADVDKTNQSYYGE+KLNYLTTDG HEGLVPLRKEGPVHDVQWS SGSEFAVVYGFMPA
Sbjct: 241 VQADVDKTNQSYYGESKLNYLTTDGTHEGLVPLRKEGPVHDVQWSCSGSEFAVVYGFMPA 300

Query: 301 RATVFDKKCKPLLELGEGPYNTVRWNPKGKFLCLAGFGNLPGDMAFWDYKEKKQLGRTKA 360
           +ATVFDKKC PLLELG GPYNT+RWNPKGKFLCLAGFGNLPGDMAFWDY EKKQLG TKA
Sbjct: 301 KATVFDKKCNPLLELGTGPYNTIRWNPKGKFLCLAGFGNLPGDMAFWDYTEKKQLGTTKA 360

Query: 361 ECSVTSEWSPDGQFFMTATTAPRLQVDNGIKIFHYNGALYFKKMFDKLFQADWKPESSDK 420
           ECSVTSEWSPDG++FMTATTAPRLQVDNGIKIFHYNG+LYFK+MFDKL+QADWKP S D 
Sbjct: 361 ECSVTSEWSPDGRYFMTATTAPRLQVDNGIKIFHYNGSLYFKRMFDKLYQADWKPVSPDM 420

Query: 421 FGEIDLLVKSVESLNVDQKKTQGQGSKVSQTSQKTAPSNPPVQKPAAYRPPHAKNAAAIQ 480
           FGEI  L++SV+SL V++ K QGQGSK +Q S KT  +NPP QKPAAYRPPHAK AA  +
Sbjct: 421 FGEISELIESVDSLKVNETKPQGQGSKTTQASTKTTFANPPAQKPAAYRPPHAKQAALEK 480

Query: 481 AELLGEGPKETLSKNAMRNKKKREKQKEKKAGEAASSA 517
           AEL GE   E +SKNA++NKKKREKQ+EKKA +AAS +
Sbjct: 481 AELFGESHTEGMSKNALKNKKKREKQREKKAADAASES 517

BLAST of CSPI04G02860 vs. TrEMBL
Match: A0A0G4AN16_9ROSI (Eukaryotic translation initiation factor eIF2A family protein OS=Francoa sonchifolia PE=2 SV=1)

HSP 1 Score: 816.2 bits (2107), Expect = 2.2e-233
Identity = 392/520 (75.38%), Postives = 450/520 (86.54%), Query Frame = 1

Query: 1   MATSQHPPPLQILVRGSDGFSIWNGPPFIDNQPTINKLDKVACTSTKFSEDGSKLLVTKS 60
           MA +   P L+ILVRG +GF +WNGPPF + QPT+ KL++V CT+ KFSEDGSKL+V KS
Sbjct: 1   MAATNPSPSLEILVRGPEGFFLWNGPPFSNGQPTV-KLERVPCTNVKFSEDGSKLIVMKS 60

Query: 61  ET-LSIYECRGFGGIKSFEVPNLLAAALSPCGTYLQTFQKSSTPQEKNVVLWKVDTGDLV 120
           ++ +SIY+C     I+SFEVPN+ AA LSP GTYLQTFQKSSTPQEKNVVLWK +TGD +
Sbjct: 61  DSVISIYDCCTSKEIRSFEVPNVSAATLSPRGTYLQTFQKSSTPQEKNVVLWKTETGDTL 120

Query: 121 YSQFQKNMTKVSWPAIRFSSDETVACRMATNEIQFFDAKDFSKGVIHRLRVPGVTAIELS 180
           Y  FQKN TK +WPAIRFSSDETVACRMATNEIQFF+  DFSKG +HRLR+PGV AIELS
Sbjct: 121 YQLFQKNTTKNTWPAIRFSSDETVACRMATNEIQFFEVGDFSKGFVHRLRIPGVAAIELS 180

Query: 181 RTPGTHIAAFVPESKGVPASVQIFACG-DSQTQPIARRSFFRCSTVQLNWNNGSTGLLIV 240
           + PGTH+AAFVPE+KG PASVQIF+CG D Q+QP+ARRSFFRCSTVQLNWN G+TGLL+V
Sbjct: 181 KAPGTHVAAFVPETKGAPASVQIFSCGKDLQSQPVARRSFFRCSTVQLNWNYGTTGLLVV 240

Query: 241 VQADVDKTNQSYYGETKLNYLTTDGVHEGLVPLRKEGPVHDVQWSQSGSEFAVVYGFMPA 300
            Q+DVDKTNQSY+GE+KL Y+TTDG HEGLVPLRKEGPVHDVQWS SGSEFAVVYGFMPA
Sbjct: 241 AQSDVDKTNQSYFGESKLYYITTDGNHEGLVPLRKEGPVHDVQWSYSGSEFAVVYGFMPA 300

Query: 301 RATVFDKKCKPLLELGEGPYNTVRWNPKGKFLCLAGFGNLPGDMAFWDYKEKKQLGRTKA 360
           +ATVFDKKC PL ELG GPYNT+RWNPKGKFLCLAGFGNLPGDMAFWDY EKKQLG TKA
Sbjct: 301 KATVFDKKCNPLFELGVGPYNTIRWNPKGKFLCLAGFGNLPGDMAFWDYIEKKQLGTTKA 360

Query: 361 ECSVTSEWSPDGQFFMTATTAPRLQVDNGIKIFHYNGALYFKKMFDKLFQADWKPESSDK 420
           E SVTSEWSPDGQ+FMTATTAPRLQVDNGIKIFHYNG+L FKKMFDKL+Q +WKPES+D+
Sbjct: 361 EWSVTSEWSPDGQYFMTATTAPRLQVDNGIKIFHYNGSLLFKKMFDKLYQVEWKPESADR 420

Query: 421 FGEIDLLVKSVESLNVDQKKTQGQGSKVSQTSQKTAPSNPPVQKPAAYRPPHAKNAAAIQ 480
           FGEI  L+KSV+SL V++ K QGQGSK +Q S K  P+ P  QKPAAYRPPHAK+AA +Q
Sbjct: 421 FGEITELIKSVDSLKVEEGKPQGQGSKSAQASAKPTPAKPAAQKPAAYRPPHAKHAAVVQ 480

Query: 481 AELLGEGPKETLSKNAMRNKKKREKQKEKKAGEAASSAND 519
           A+LLGE   ET+SKNA+RNKKKREKQ+EKK  + +S+ +D
Sbjct: 481 AQLLGESSAETMSKNALRNKKKREKQREKKTSDTSSAGSD 519

BLAST of CSPI04G02860 vs. TrEMBL
Match: A0A059AUL5_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_H00311 PE=4 SV=1)

HSP 1 Score: 815.1 bits (2104), Expect = 5.0e-233
Identity = 385/520 (74.04%), Postives = 453/520 (87.12%), Query Frame = 1

Query: 1   MATSQHPPPLQILVRGSDGFSIWNGPPFIDNQPTINKLDKVACTSTKFSEDGSKLLVTKS 60
           MAT+   PPL ILVR  +GFS+WNGPPF + QP++ KLD+  CTST FS DGS+L+V +S
Sbjct: 93  MATTASSPPLHILVREPEGFSLWNGPPFANGQPSV-KLDRTPCTSTMFSTDGSRLMVIRS 152

Query: 61  ET-LSIYECRGFGGIKSFEVPNLLAAALSPCGTYLQTFQKSSTPQEKNVVLWKVDTGDLV 120
           ++ + +Y+C  F  I++FE+PNL+ AALSPCGTYLQTFQKSSTPQEKNV+LWKV++G+ V
Sbjct: 153 DSAVGVYDCSNFKEIRNFEIPNLVTAALSPCGTYLQTFQKSSTPQEKNVILWKVESGESV 212

Query: 121 YSQFQKNMTKVSWPAIRFSSDETVACRMATNEIQFFDAKDFSKGVIHRLRVPGVTAIELS 180
           Y   QKN+TK +WP+IRFSSDE VACR+ATNEIQFFD  DFSKG  +RLRVPGV A+ELS
Sbjct: 213 YQLLQKNITKTTWPSIRFSSDEAVACRLATNEIQFFDGCDFSKGFKYRLRVPGVAAVELS 272

Query: 181 RTPGTHIAAFVPESKGVPASVQIFACG-DSQTQPIARRSFFRCSTVQLNWNNGSTGLLIV 240
           +TPG+HIAAFVPESKGVPASVQIFAC  D Q+QP+ARRSFFRCS+VQL+WN GSTGLL+V
Sbjct: 273 KTPGSHIAAFVPESKGVPASVQIFACREDQQSQPVARRSFFRCSSVQLHWNYGSTGLLVV 332

Query: 241 VQADVDKTNQSYYGETKLNYLTTDGVHEGLVPLRKEGPVHDVQWSQSGSEFAVVYGFMPA 300
           VQ+DVDKTNQSYYGE+KLNYLTTDG HEGLVPLRKEGP+HDVQWS SGSEFAVVYGFMPA
Sbjct: 333 VQSDVDKTNQSYYGESKLNYLTTDGAHEGLVPLRKEGPIHDVQWSYSGSEFAVVYGFMPA 392

Query: 301 RATVFDKKCKPLLELGEGPYNTVRWNPKGKFLCLAGFGNLPGDMAFWDYKEKKQLGRTKA 360
           +AT+FDKKC PLLELG GPYNT+RWNPKGKF+CLAGFGNLPGDMAFWDY+EKKQLG TKA
Sbjct: 393 KATIFDKKCNPLLELGTGPYNTIRWNPKGKFICLAGFGNLPGDMAFWDYREKKQLGATKA 452

Query: 361 ECSVTSEWSPDGQFFMTATTAPRLQVDNGIKIFHYNGALYFKKMFDKLFQADWKPESSDK 420
           ECSVTSEWSP+G +FMTATTAPRLQ+DN IKIFH+NG+L+FKKMFDKL+QA+WK ES + 
Sbjct: 453 ECSVTSEWSPNGCYFMTATTAPRLQIDNCIKIFHHNGSLFFKKMFDKLYQAEWKQESPES 512

Query: 421 FGEIDLLVKSVESLNVDQKKTQGQGSKVSQTSQKTAPSNPPVQKPAAYRPPHAKNAAAIQ 480
           FGE+  L+KS++SL V+++K QGQ ++ SQ S K   +N P QKPAAYRPPHAKNAA +Q
Sbjct: 513 FGEVAELIKSIDSLKVEEQKPQGQTARSSQNSAKVTSTNMPAQKPAAYRPPHAKNAATVQ 572

Query: 481 AELLGEGPKETLSKNAMRNKKKREKQKEKKAGEAASSAND 519
           AEL GE   ETLSKNA+RNKKKREKQ+EKKA EA S+ +D
Sbjct: 573 AELFGESSSETLSKNALRNKKKREKQREKKAAEAGSALSD 611

BLAST of CSPI04G02860 vs. TAIR10
Match: AT1G73180.1 (AT1G73180.1 Eukaryotic translation initiation factor eIF2A family protein)

HSP 1 Score: 728.4 bits (1879), Expect = 3.1e-210
Identity = 344/513 (67.06%), Postives = 423/513 (82.46%), Query Frame = 1

Query: 8   PPLQILVRGSDGFSIWNGPPFIDNQPTINKLDKVACTSTKFSEDGSKLLVTKSE-TLSIY 67
           P L+ILVR ++GF++WNGPPF + QPT+ KL++V+C++TKFS DGSKL+  KS+  +SIY
Sbjct: 5   PSLEILVREAEGFTVWNGPPFTNGQPTL-KLERVSCSNTKFSVDGSKLMAMKSDGVISIY 64

Query: 68  ECRGFGGIKSFEVPNLLAAALSPCGTYLQTFQKSSTPQEKNVVLWKVDTGDLVYSQFQKN 127
           +      ++SF + N+ AA LSPCGTYLQTFQK +TPQEKNV +W  +TGDL +S +QK+
Sbjct: 65  DATSLTEVRSFTIANVTAAELSPCGTYLQTFQKPTTPQEKNVSIWNTETGDLAHSHYQKS 124

Query: 128 MTKVSWPAIRFSSDETVACRMATNEIQFFDAKDFSKGVIHRLRVPGVTAIELSRTPGTHI 187
           +TK SWP+IRF  DE+ ACR+ATNE+QFFD KDFSKG+  R+RVPG+ A ELS+TP +H+
Sbjct: 125 ITKASWPSIRFCPDESSACRLATNEVQFFDPKDFSKGITSRIRVPGIAAFELSKTPASHV 184

Query: 188 AAFVPESKGVPASVQIFACGDS-QTQPIARRSFFRCSTVQLNWNNGSTGLLIVVQADVDK 247
           A FVPESKG P SVQIF CG+  Q+QP ARRSFFRCS+VQ +WN+GSTGLL+VVQ+DVDK
Sbjct: 185 AVFVPESKGSPGSVQIFGCGEELQSQPSARRSFFRCSSVQFSWNHGSTGLLVVVQSDVDK 244

Query: 248 TNQSYYGETKLNYLTTDGVHEGLVPLRKEGPVHDVQWSQSGSEFAVVYGFMPARATVFDK 307
           TNQSYYGETKL+YLT DG HEGLVPLRKEGPVHDVQWS SGSEFAVVYGFMPA  T+FDK
Sbjct: 245 TNQSYYGETKLHYLTIDGTHEGLVPLRKEGPVHDVQWSFSGSEFAVVYGFMPACVTIFDK 304

Query: 308 KCKPLLELGEGPYNTVRWNPKGKFLCLAGFGNLPGDMAFWDYKEKKQLGRTKAECSVTSE 367
            CKPL+ELGEGPYNT+RWNPKG+ LC+AGFGNLPGDMAFWD   KKQLG  KAE SVTSE
Sbjct: 305 NCKPLMELGEGPYNTLRWNPKGRVLCVAGFGNLPGDMAFWDVVNKKQLGSNKAEWSVTSE 364

Query: 368 WSPDGQFFMTATTAPRLQVDNGIKIFHYNGALYFKKMFDKLFQADWKPESSDKFGEIDLL 427
           WSPDG++F+TA+TAPR Q+DNG+KIF+Y+G  Y+KKMF++L+QA+WKPES ++FG+I  L
Sbjct: 365 WSPDGRYFLTASTAPRRQIDNGMKIFNYDGKRYYKKMFERLYQAEWKPESPERFGDISEL 424

Query: 428 VKSVESLNVDQKKTQGQGSKVSQTSQKTAPSNPPVQKPAAYRPPHAKNAAAIQAELLGEG 487
           +KSVESL + + K+QGQGS      +KT   NP  QKPAAYRPPHAK+AAA+QAELLG  
Sbjct: 425 IKSVESLKLGEGKSQGQGS----AQKKTIAPNPIAQKPAAYRPPHAKHAAAVQAELLGIS 484

Query: 488 PKETLSKNAMRNKKKREKQKEKKAGEAASSAND 519
               +SKNA++NKKKRE +K   A  AAS +N+
Sbjct: 485 TTGEMSKNALKNKKKRENKKAAAAAAAASGSNN 512

BLAST of CSPI04G02860 vs. NCBI nr
Match: gi|778689907|ref|XP_011653038.1| (PREDICTED: eukaryotic translation initiation factor 2A [Cucumis sativus])

HSP 1 Score: 1050.4 bits (2715), Expect = 1.0e-303
Identity = 516/519 (99.42%), Postives = 517/519 (99.61%), Query Frame = 1

Query: 1   MATSQHPPPLQILVRGSDGFSIWNGPPFIDNQPTINKLDKVACTSTKFSEDGSKLLVTKS 60
           MATSQHPPPLQILVRGSDGFSIWNGPPFIDNQPTINKLDKVACTSTKFSEDGSKLLVTKS
Sbjct: 1   MATSQHPPPLQILVRGSDGFSIWNGPPFIDNQPTINKLDKVACTSTKFSEDGSKLLVTKS 60

Query: 61  ETLSIYECRGFGGIKSFEVPNLLAAALSPCGTYLQTFQKSSTPQEKNVVLWKVDTGDLVY 120
           ETL+IYECRGFG IKSFEVPNLLAAALSPCGTYLQTFQKSSTPQEKNVVLWKVDTG LVY
Sbjct: 61  ETLTIYECRGFGEIKSFEVPNLLAAALSPCGTYLQTFQKSSTPQEKNVVLWKVDTGGLVY 120

Query: 121 SQFQKNMTKVSWPAIRFSSDETVACRMATNEIQFFDAKDFSKGVIHRLRVPGVTAIELSR 180
           SQFQKNMTKVSWPAIRFSSDETVACRMATNEIQFFDAKDFSKGVIHRLRVPGVTAIELSR
Sbjct: 121 SQFQKNMTKVSWPAIRFSSDETVACRMATNEIQFFDAKDFSKGVIHRLRVPGVTAIELSR 180

Query: 181 TPGTHIAAFVPESKGVPASVQIFACGDSQTQPIARRSFFRCSTVQLNWNNGSTGLLIVVQ 240
           TPGTHIAAFVPESKGVPASVQIFACGDSQTQPIARRSFFRCSTVQLNWNNGSTGLLIVVQ
Sbjct: 181 TPGTHIAAFVPESKGVPASVQIFACGDSQTQPIARRSFFRCSTVQLNWNNGSTGLLIVVQ 240

Query: 241 ADVDKTNQSYYGETKLNYLTTDGVHEGLVPLRKEGPVHDVQWSQSGSEFAVVYGFMPARA 300
           ADVDKTNQSYYGETKLNYLTTDGVHEGLVPLRKEGPVHDVQWSQSGSEFAVVYGFMPARA
Sbjct: 241 ADVDKTNQSYYGETKLNYLTTDGVHEGLVPLRKEGPVHDVQWSQSGSEFAVVYGFMPARA 300

Query: 301 TVFDKKCKPLLELGEGPYNTVRWNPKGKFLCLAGFGNLPGDMAFWDYKEKKQLGRTKAEC 360
           TVFDKKCKPLLELGEGPYNTVRWNPKGKFLCLAGFGNLPGDMAFWDYKEKKQLGRTKAEC
Sbjct: 301 TVFDKKCKPLLELGEGPYNTVRWNPKGKFLCLAGFGNLPGDMAFWDYKEKKQLGRTKAEC 360

Query: 361 SVTSEWSPDGQFFMTATTAPRLQVDNGIKIFHYNGALYFKKMFDKLFQADWKPESSDKFG 420
           SVTSEWSPDGQFFMTATTAPRLQVDNGIKIFHYNGALYFKKMFDKLFQADWKPESSDKFG
Sbjct: 361 SVTSEWSPDGQFFMTATTAPRLQVDNGIKIFHYNGALYFKKMFDKLFQADWKPESSDKFG 420

Query: 421 EIDLLVKSVESLNVDQKKTQGQGSKVSQTSQKTAPSNPPVQKPAAYRPPHAKNAAAIQAE 480
           EIDLLVKSVESLNVDQKKTQGQGSKVSQTSQKTAPSNPPVQKPAAYRPPHAKNAAAIQAE
Sbjct: 421 EIDLLVKSVESLNVDQKKTQGQGSKVSQTSQKTAPSNPPVQKPAAYRPPHAKNAAAIQAE 480

Query: 481 LLGEGPKETLSKNAMRNKKKREKQKEKKAGEAASSANDV 520
           LLGEGPKETLSKNAMRNKKKREKQKEKKAGEAASSANDV
Sbjct: 481 LLGEGPKETLSKNAMRNKKKREKQKEKKAGEAASSANDV 519

BLAST of CSPI04G02860 vs. NCBI nr
Match: gi|659108148|ref|XP_008454042.1| (PREDICTED: eukaryotic translation initiation factor 2A [Cucumis melo])

HSP 1 Score: 1040.0 bits (2688), Expect = 1.4e-300
Identity = 508/519 (97.88%), Postives = 513/519 (98.84%), Query Frame = 1

Query: 1   MATSQHPPPLQILVRGSDGFSIWNGPPFIDNQPTINKLDKVACTSTKFSEDGSKLLVTKS 60
           MATSQHPPPL+ILVRGSDGFSIWNGPPF+DNQPT  KLDKVACTSTKFSEDGSKLLVTKS
Sbjct: 1   MATSQHPPPLEILVRGSDGFSIWNGPPFVDNQPTTKKLDKVACTSTKFSEDGSKLLVTKS 60

Query: 61  ETLSIYECRGFGGIKSFEVPNLLAAALSPCGTYLQTFQKSSTPQEKNVVLWKVDTGDLVY 120
           ETLSIYECRGFG IKSFEVPNLLAAALSPCGTYLQTFQKSSTPQEKNVVLWKVDTGDLVY
Sbjct: 61  ETLSIYECRGFGEIKSFEVPNLLAAALSPCGTYLQTFQKSSTPQEKNVVLWKVDTGDLVY 120

Query: 121 SQFQKNMTKVSWPAIRFSSDETVACRMATNEIQFFDAKDFSKGVIHRLRVPGVTAIELSR 180
           SQFQKNMTKVSWPAIRFSSDETVACRMATNEIQFFD KDFSKGVIHRLRVPGV AIELSR
Sbjct: 121 SQFQKNMTKVSWPAIRFSSDETVACRMATNEIQFFDTKDFSKGVIHRLRVPGVVAIELSR 180

Query: 181 TPGTHIAAFVPESKGVPASVQIFACGDSQTQPIARRSFFRCSTVQLNWNNGSTGLLIVVQ 240
           TPGTHIAAFVPESKGVPAS+QIFACGDSQTQPIARRSFFRCSTVQLNWNNGSTGLLIVVQ
Sbjct: 181 TPGTHIAAFVPESKGVPASIQIFACGDSQTQPIARRSFFRCSTVQLNWNNGSTGLLIVVQ 240

Query: 241 ADVDKTNQSYYGETKLNYLTTDGVHEGLVPLRKEGPVHDVQWSQSGSEFAVVYGFMPARA 300
           ADVDKTNQSYYGETKLNYLTTDGVHEGLVPLRKEGPVHDVQWS SGSEFAVVYGFMPARA
Sbjct: 241 ADVDKTNQSYYGETKLNYLTTDGVHEGLVPLRKEGPVHDVQWSPSGSEFAVVYGFMPARA 300

Query: 301 TVFDKKCKPLLELGEGPYNTVRWNPKGKFLCLAGFGNLPGDMAFWDYKEKKQLGRTKAEC 360
           TVFDKKCKPLLELGEGPYNTVRWNPKGKFLCLAGFGNLPGDMAFWDYKEKKQLGRTK+EC
Sbjct: 301 TVFDKKCKPLLELGEGPYNTVRWNPKGKFLCLAGFGNLPGDMAFWDYKEKKQLGRTKSEC 360

Query: 361 SVTSEWSPDGQFFMTATTAPRLQVDNGIKIFHYNGALYFKKMFDKLFQADWKPESSDKFG 420
           SVTSEWSPDGQ+FMTATTAPRLQVDNGIKIFHYNGALYFKKMFDKLFQADWKPESSDKFG
Sbjct: 361 SVTSEWSPDGQYFMTATTAPRLQVDNGIKIFHYNGALYFKKMFDKLFQADWKPESSDKFG 420

Query: 421 EIDLLVKSVESLNVDQKKTQGQGSKVSQTSQKTAPSNPPVQKPAAYRPPHAKNAAAIQAE 480
           EIDLLVKSVESLNVDQKKTQGQGSKVSQTSQKTAPSNPPVQKPAAYRPPHAKNAAAIQAE
Sbjct: 421 EIDLLVKSVESLNVDQKKTQGQGSKVSQTSQKTAPSNPPVQKPAAYRPPHAKNAAAIQAE 480

Query: 481 LLGEGPKETLSKNAMRNKKKREKQKEKKAGEAASSANDV 520
           LLGEGPKETLSKNAMRNKKKREKQKEKKAGEAASSANDV
Sbjct: 481 LLGEGPKETLSKNAMRNKKKREKQKEKKAGEAASSANDV 519

BLAST of CSPI04G02860 vs. NCBI nr
Match: gi|596164770|ref|XP_007222982.1| (hypothetical protein PRUPE_ppa004316mg [Prunus persica])

HSP 1 Score: 825.1 bits (2130), Expect = 6.9e-236
Identity = 395/516 (76.55%), Postives = 454/516 (87.98%), Query Frame = 1

Query: 1   MATSQHPPPLQILVRGSDGFSIWNGPPFIDNQPTINKLDKVACTSTKFSEDGSKLLVTKS 60
           MA S+  PPL ILVRG +GFS+WNGPPF + QP I KL+ ++CTSTKFSEDGS+L+V KS
Sbjct: 1   MAASETAPPLDILVRGPEGFSLWNGPPFSNGQPGI-KLENISCTSTKFSEDGSRLMVMKS 60

Query: 61  ET-LSIYECRGFGGIKSFEVPNLLAAALSPCGTYLQTFQKSSTPQEKNVVLWKVDTGDLV 120
           ++ +SIY+C  +  I+SF++PN+ AAALSP GTY+QTFQKSSTPQEKNV+LW  +TGD V
Sbjct: 61  DSVISIYDCSSYREIRSFQIPNVAAAALSPRGTYVQTFQKSSTPQEKNVILWNTETGDPV 120

Query: 121 YSQFQKNMTKVSWPAIRFSSDETVACRMATNEIQFFDAKDFSKGVIHRLRVPGVTAIELS 180
           Y QFQKNMTK +WP+IRFSSDE VACR+ATNEIQF DA DFSK  I+RLRVPGV A ELS
Sbjct: 121 YQQFQKNMTKATWPSIRFSSDEAVACRLATNEIQFLDAGDFSKPFIYRLRVPGVAAFELS 180

Query: 181 RTPGTHIAAFVPESKGVPASVQIFACGDS-QTQPIARRSFFRCSTVQLNWNNGSTGLLIV 240
           + PG+H+AAFVPESKG+PASVQIF+C  + Q+QP+ARRSFFRCS+VQLNWNNGSTGLL+V
Sbjct: 181 KAPGSHVAAFVPESKGIPASVQIFSCAKAVQSQPLARRSFFRCSSVQLNWNNGSTGLLVV 240

Query: 241 VQADVDKTNQSYYGETKLNYLTTDGVHEGLVPLRKEGPVHDVQWSQSGSEFAVVYGFMPA 300
           VQ+DVDKTNQSYYGE+KLNYLTTDG HEGLVPLRKEGPVHDVQWS SGSEFAVVYGFMP+
Sbjct: 241 VQSDVDKTNQSYYGESKLNYLTTDGTHEGLVPLRKEGPVHDVQWSYSGSEFAVVYGFMPS 300

Query: 301 RATVFDKKCKPLLELGEGPYNTVRWNPKGKFLCLAGFGNLPGDMAFWDYKEKKQLGRTKA 360
           +ATVFDKKC PLLELG GPYNT+RWNPKGKFLCLAGFGNLPGDMAFWDY EKKQLG T+A
Sbjct: 301 KATVFDKKCNPLLELGTGPYNTIRWNPKGKFLCLAGFGNLPGDMAFWDYGEKKQLGTTRA 360

Query: 361 ECSVTSEWSPDGQFFMTATTAPRLQVDNGIKIFHYNGALYFKKMFDKLFQADWKPESSDK 420
           E SVTSEWSPDG +FMTATTAPRLQVDNGIKIFH+NG+L+FKKMFDKL+QADWKPES D+
Sbjct: 361 ELSVTSEWSPDGCYFMTATTAPRLQVDNGIKIFHHNGSLFFKKMFDKLYQADWKPESPDR 420

Query: 421 FGEIDLLVKSVESLNVDQKKTQGQGSKVSQTSQKTAPSNPPVQKPAAYRPPHAKNAAAIQ 480
           FG+I  LVKS+ SL V + K QG  SK+SQTS K + SNPP QKPAAYRPPHAK+AAA+Q
Sbjct: 421 FGDIAELVKSIGSLKVVETKPQGDRSKISQTSAKASASNPPAQKPAAYRPPHAKSAAAVQ 480

Query: 481 AELLGEGPKETLSKNAMRNKKKREKQKEKKAGEAAS 515
           A+L GE   ET+SKNA++NKKKREKQ+EKKA EA S
Sbjct: 481 AQLFGESSTETVSKNALKNKKKREKQREKKAAEATS 515

BLAST of CSPI04G02860 vs. NCBI nr
Match: gi|645231668|ref|XP_008222503.1| (PREDICTED: eukaryotic translation initiation factor 2A [Prunus mume])

HSP 1 Score: 824.3 bits (2128), Expect = 1.2e-235
Identity = 395/516 (76.55%), Postives = 454/516 (87.98%), Query Frame = 1

Query: 1   MATSQHPPPLQILVRGSDGFSIWNGPPFIDNQPTINKLDKVACTSTKFSEDGSKLLVTKS 60
           MA S+  PPL ILVRG +GFS+WNGPPF + QP I KL+ ++CTSTKFSEDGS+L+V KS
Sbjct: 1   MAASETAPPLDILVRGPEGFSLWNGPPFSNGQPGI-KLENISCTSTKFSEDGSRLMVMKS 60

Query: 61  ET-LSIYECRGFGGIKSFEVPNLLAAALSPCGTYLQTFQKSSTPQEKNVVLWKVDTGDLV 120
           ++ +SIY+C  +  I+SF++PN+ AAALSP GTYLQTFQKSSTPQEKNV+LW  +TGD V
Sbjct: 61  DSVISIYDCSSYREIRSFQIPNVAAAALSPRGTYLQTFQKSSTPQEKNVILWNTETGDPV 120

Query: 121 YSQFQKNMTKVSWPAIRFSSDETVACRMATNEIQFFDAKDFSKGVIHRLRVPGVTAIELS 180
           Y QFQKNMTK +WP+IRFSSDE VACR+ATNEIQF DA DFSK  I+RLRVPGV A ELS
Sbjct: 121 YQQFQKNMTKATWPSIRFSSDEAVACRLATNEIQFLDAGDFSKPFIYRLRVPGVAAFELS 180

Query: 181 RTPGTHIAAFVPESKGVPASVQIFACGDS-QTQPIARRSFFRCSTVQLNWNNGSTGLLIV 240
           + PG+H+AAFVPESKG+PASVQIF+C  + Q+QP+ARRSFFRCS+VQL+WNNGSTGLL+V
Sbjct: 181 KAPGSHVAAFVPESKGIPASVQIFSCAKAVQSQPLARRSFFRCSSVQLHWNNGSTGLLVV 240

Query: 241 VQADVDKTNQSYYGETKLNYLTTDGVHEGLVPLRKEGPVHDVQWSQSGSEFAVVYGFMPA 300
           VQ+DVDKTNQSYYGE+KLNYLTTDG HEGLVPLRKEGPVHDVQW+ SGSEFAVVYGFMP+
Sbjct: 241 VQSDVDKTNQSYYGESKLNYLTTDGTHEGLVPLRKEGPVHDVQWAYSGSEFAVVYGFMPS 300

Query: 301 RATVFDKKCKPLLELGEGPYNTVRWNPKGKFLCLAGFGNLPGDMAFWDYKEKKQLGRTKA 360
           +ATVFDKKC PLLELG GPYNT+RWNPKGKFLCLAGFGNLPGDMAFWDY EKKQLG T+A
Sbjct: 301 KATVFDKKCNPLLELGTGPYNTIRWNPKGKFLCLAGFGNLPGDMAFWDYVEKKQLGTTRA 360

Query: 361 ECSVTSEWSPDGQFFMTATTAPRLQVDNGIKIFHYNGALYFKKMFDKLFQADWKPESSDK 420
           E SVTSEWSPDG +FMTATTAPRLQVDNGIKIFH+NG+L+FKKMFDKL+QADWKPES D+
Sbjct: 361 ELSVTSEWSPDGCYFMTATTAPRLQVDNGIKIFHHNGSLFFKKMFDKLYQADWKPESPDR 420

Query: 421 FGEIDLLVKSVESLNVDQKKTQGQGSKVSQTSQKTAPSNPPVQKPAAYRPPHAKNAAAIQ 480
           FG+I  LVKS+ SL V + K QG  SK+SQTS K + SNPP QKPAAYRPPHAK+AAA+Q
Sbjct: 421 FGDIAELVKSIGSLKVVETKPQGDRSKISQTSAKASASNPPAQKPAAYRPPHAKSAAAVQ 480

Query: 481 AELLGEGPKETLSKNAMRNKKKREKQKEKKAGEAAS 515
           A+L GE   ET+SKNA++NKKKREKQKEKKA EA S
Sbjct: 481 AQLFGESSTETVSKNALKNKKKREKQKEKKAAEATS 515

BLAST of CSPI04G02860 vs. NCBI nr
Match: gi|836712587|gb|AKM76406.1| (eukaryotic translation initiation factor eIF2A family protein [Hypseocharis bilobata])

HSP 1 Score: 823.2 bits (2125), Expect = 2.6e-235
Identity = 393/518 (75.87%), Postives = 455/518 (87.84%), Query Frame = 1

Query: 1   MATSQHPPPLQILVRGSDGFSIWNGPPFIDNQPTINKLDKVACTSTKFSEDGSKLLVTKS 60
           MA +   P L+IL RG +GFS+WNGPPF + QP+I KL++V CT+ KFSEDGS+L+VTKS
Sbjct: 1   MAATDRSPTLEILARGPEGFSLWNGPPFSNGQPSI-KLERVPCTNAKFSEDGSRLMVTKS 60

Query: 61  ETL-SIYECRGFGGIKSFEVPNLLAAALSPCGTYLQTFQKSSTPQEKNVVLWKVDTGDLV 120
           +++ SIY+C     I++F+VPN+LAAALSP GTYLQTFQK STPQEKNVVLW  +TGD V
Sbjct: 61  DSVVSIYDCSTSKEIRTFQVPNILAAALSPRGTYLQTFQKFSTPQEKNVVLWNTETGDPV 120

Query: 121 YSQFQKNMTKVSWPAIRFSSDETVACRMATNEIQFFDAKDFSKGVIHRLRVPGVTAIELS 180
           Y QFQKNMTK +WP+IRFSSDE +ACR+ATNEIQFF+ +DFSKG ++RLRVPGV A+ELS
Sbjct: 121 YQQFQKNMTKTTWPSIRFSSDEAIACRLATNEIQFFNGRDFSKGTVNRLRVPGVAAVELS 180

Query: 181 RTPGTHIAAFVPESKGVPASVQIFACG-DSQTQPIARRSFFRCSTVQLNWNNGSTGLLIV 240
           + PG+H+AA+VPESKGVPASVQI+AC  D Q+QP+ARRSFFRCS+VQLNWN+GSTGLL++
Sbjct: 181 KAPGSHVAAYVPESKGVPASVQIYACEKDLQSQPVARRSFFRCSSVQLNWNHGSTGLLVL 240

Query: 241 VQADVDKTNQSYYGETKLNYLTTDGVHEGLVPLRKEGPVHDVQWSQSGSEFAVVYGFMPA 300
           VQADVDKTNQSYYGE+KLNYLTTDG HEGLVPLRKEGPVHDVQWS SGSEFAVVYGFMPA
Sbjct: 241 VQADVDKTNQSYYGESKLNYLTTDGTHEGLVPLRKEGPVHDVQWSCSGSEFAVVYGFMPA 300

Query: 301 RATVFDKKCKPLLELGEGPYNTVRWNPKGKFLCLAGFGNLPGDMAFWDYKEKKQLGRTKA 360
           +ATVFDKKC PLLELG GPYNT+RWNPKGKFLCLAGFGNLPGDMAFWDY EKKQLG TKA
Sbjct: 301 KATVFDKKCNPLLELGTGPYNTIRWNPKGKFLCLAGFGNLPGDMAFWDYTEKKQLGTTKA 360

Query: 361 ECSVTSEWSPDGQFFMTATTAPRLQVDNGIKIFHYNGALYFKKMFDKLFQADWKPESSDK 420
           ECSVTSEWSPDG++FMTATTAPRLQVDNGIKIFHYNG+LYFK+MFDKL+QADWKP S D 
Sbjct: 361 ECSVTSEWSPDGRYFMTATTAPRLQVDNGIKIFHYNGSLYFKRMFDKLYQADWKPVSPDM 420

Query: 421 FGEIDLLVKSVESLNVDQKKTQGQGSKVSQTSQKTAPSNPPVQKPAAYRPPHAKNAAAIQ 480
           FGEI  L++SV+SL V++ K QGQGSK +Q S KT  +NPP QKPAAYRPPHAK AA  +
Sbjct: 421 FGEISELIESVDSLKVNETKPQGQGSKTTQASTKTTFANPPAQKPAAYRPPHAKQAALEK 480

Query: 481 AELLGEGPKETLSKNAMRNKKKREKQKEKKAGEAASSA 517
           AEL GE   E +SKNA++NKKKREKQ+EKKA +AAS +
Sbjct: 481 AELFGESHTEGMSKNALKNKKKREKQREKKAADAASES 517

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
EIF2A_CHICK2.9e-7734.45Eukaryotic translation initiation factor 2A OS=Gallus gallus GN=EIF2A PE=2 SV=1[more]
EIF2A_MOUSE3.8e-7734.52Eukaryotic translation initiation factor 2A OS=Mus musculus GN=Eif2a PE=1 SV=2[more]
EIF2A_DANRE6.5e-7734.26Eukaryotic translation initiation factor 2A OS=Danio rerio GN=eif2a PE=2 SV=1[more]
EIF2A_XENLA2.5e-7633.89Eukaryotic translation initiation factor 2A OS=Xenopus laevis GN=eif2a PE=2 SV=2[more]
EIF2A_HUMAN1.2e-7533.21Eukaryotic translation initiation factor 2A OS=Homo sapiens GN=EIF2A PE=1 SV=3[more]
Match NameE-valueIdentityDescription
A0A0A0KXB3_CUCSA7.0e-30499.42Uncharacterized protein OS=Cucumis sativus GN=Csa_4G011690 PE=4 SV=1[more]
M5XFE3_PRUPE4.8e-23676.55Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa004316mg PE=4 SV=1[more]
A0A0G4AP04_9ROSI1.8e-23575.87Eukaryotic translation initiation factor eIF2A family protein OS=Hypseocharis bi... [more]
A0A0G4AN16_9ROSI2.2e-23375.38Eukaryotic translation initiation factor eIF2A family protein OS=Francoa sonchif... [more]
A0A059AUL5_EUCGR5.0e-23374.04Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_H00311 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT1G73180.13.1e-21067.06 Eukaryotic translation initiation factor eIF2A family protein[more]
Match NameE-valueIdentityDescription
gi|778689907|ref|XP_011653038.1|1.0e-30399.42PREDICTED: eukaryotic translation initiation factor 2A [Cucumis sativus][more]
gi|659108148|ref|XP_008454042.1|1.4e-30097.88PREDICTED: eukaryotic translation initiation factor 2A [Cucumis melo][more]
gi|596164770|ref|XP_007222982.1|6.9e-23676.55hypothetical protein PRUPE_ppa004316mg [Prunus persica][more]
gi|645231668|ref|XP_008222503.1|1.2e-23576.55PREDICTED: eukaryotic translation initiation factor 2A [Prunus mume][more]
gi|836712587|gb|AKM76406.1|2.6e-23575.87eukaryotic translation initiation factor eIF2A family protein [Hypseocharis bilo... [more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR011387TIF2A
IPR013979TIF_beta_prop-like
IPR015943WD40/YVTN_repeat-like_dom_sf
Vocabulary: Molecular Function
TermDefinition
GO:0005515protein binding
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006446 regulation of translational initiation
biological_process GO:0006417 regulation of translation
biological_process GO:0006413 translational initiation
cellular_component GO:0005886 plasma membrane
cellular_component GO:0005840 ribosome
cellular_component GO:0005575 cellular_component
molecular_function GO:0005515 protein binding
molecular_function GO:0003743 translation initiation factor activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI04G02860.1CSPI04G02860.1mRNA


Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011387Translation initiation factor 2APIRPIRSF017222Transl_init_eIF2Acoord: 4..518
score: 5.4E
IPR013979Translation initiation factor, beta propellor-like domainPFAMPF08662eIF2Acoord: 216..409
score: 1.3
IPR015943WD40/YVTN repeat-like-containing domainGENE3DG3DSA:2.130.10.10coord: 36..169
score: 2.2E-6coord: 272..393
score: 3.
NoneNo IPR availableunknownCoilCoilcoord: 487..507
scor
NoneNo IPR availablePANTHERPTHR13227NUCLEASE-RELATEDcoord: 1..514
score: 6.0E
NoneNo IPR availableunknownSSF82171DPP6 N-terminal domain-likecoord: 36..160
score: 8.04E-21coord: 272..394
score: 8.04