CSPI04G00960 (gene) Wild cucumber (PI 183967)

NameCSPI04G00960
Typegene
OrganismCucumis sativus (Wild cucumber (PI 183967))
DescriptionEpoxide hydrolase, putative
LocationChr4 : 509716 .. 512196 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CAAAATGTGCGGTCAACAAAGATGGCGATTCTCCAAATTTAATATTCCAAAACCAGACTCAGCCATGAGATTCTCCAATCTCAATCTCGAGCTCTTTCTTTTCCCATGTAAACTGGTCTTTTCTACTTTCTGCCCGATCTTTCTACTTTCTACACTCCTCTCTGGCACACCCAGAAATTCAAACTTGAAGCAGAGATAGAGAGAGTGAGTTTTCCATGGATGCGATTCAACACACCACCATCAACACTAATGGCATCAAAATGCATATTGCCTCAATAGGCAATGGCCCCGTCGTTCTTCTTCTTCATGGCTTCCCCGAGCTGTGGTACTCATGGCGCCACCAGCTTCTCTACCTTTCCTCCGTCGGGTATCGAGCCATCGCGCCGGACCTCCGAGGCTATGGCGACACGGACTCCCCTGGATCGCATACTTCCTACACCGCCCTCCATATTGTCGGCGATTTGATTGGGGCTTTGGATGAGCTTGGGATTGAGAAGGTGTTTCTTGTAGGCCATGACTGGGGGGCGATCATTGCTTGGTACTTTTGCTTGTTCAGGCCTGAAAGAATCAAAGCCTTGGTGAATCTGAGCGTCCAGTTCTTCCCTAGAAATCCGGCTATTTCTTTCATCCAGCGTTTCAGAGCTGCGTATGGTGATGATTTCTATATGTGCAGGTTTCAGGTGAATCTGAACTTTGATTAATTTCTAACTATGGATTTAATTAGGGATATTCCCTTCTGATTCTGCTCCTAATTTCGATTAATGTCTATGCTTGTATTTTAGTTGCTTATTAGTTGGATGAATGATGTTATCAATTTGCTTAGTTTTGGTGTTCTGGTGTGTAGATTGACTCTGTTCAGTCTCACTCAGACGCACAATCGAAGTTGCAAAATACCGATTGTGTTCAATTAAAGTACTGTTGATGAATCAAAGCTTAAAAGATTGTGTTCTTAACATGTTCATTGTAAAGTTCTTTATAGTTTTTTGGCTGTTTAACTGTTGTATCTTACCATTCTCCCATGTAAATGATTGCAAAATGTCCTGAGAAAGACTTTGATAGAAGTTACTGAGTTCATTTAGTTGGAAGATTCCTTCTTGCTAATACATTCAATTAACAAATAATACTGAAACAATTTTCACCTTTTTTTGTGGTTCTCTCTTGATTCATCAAGTTGCTGATTAGACGGATAGCCCCATCTAAATTTTGTTTGATTCTCTGTTTTGATCAACAATTTTCTCCAAGTTGGAAAGCCATGTCGAGCTTTTCTGAGTGTAAAGTCTTTCTATCGAACAAGAAAGCTGAAATTATAGCAATTATTGGTTTGGACCTTTTATTTTTTTACTAATAAAACAATTTTTCATATCATTTATGTAATCGTAGGGGATGCTCATGTAACCAGGAAAAATACAAATGAATTGGCTGTGGTTTCTAGAAATTCATGGCATCTAAAACATGTGTAGACTGATTTCTAACAGGTTCCAGGAGAGGCAGAAGCAGATTTTGCCTGCATTGACACTGTTCAACTGTTCAAGACAACACTGTCTACTAGAAGTACAGAGGCACCTTGTTTGCCTAAAGAATACGGATTTCGCGCCATCCCACCGCCAGAAAACCTTCCTTCTTGGTTGACAGAAGAAGATATCAACTATTATGCTGCCAAATTCAAGGAGACAGGCTTCACTGGAGCATTGAACTACTATCGCGCTTTTGACTTGTAAGTCACGTTTGTTTACATCCGAGTTCGACGTTAATATTATTAGATAACATGAGGTTTCAACTAAAGAACAATTGGTAACAGAAAAGTAATATATCTTATAAACATTTTGAGACCTTTTGATTTTTCCAACTTCCAATCCTCAACACATGACTCCGTCTGACACACTTTTCTGTGTTTCATTTTCAACAACCGTTTCGTGTTTTGACATTGAATAGTTGTTTTATTCGCATTTTCATAGCAAGAAACTGTTGAGTGGTGAATTGTTTCTGTTTTTCCTCACTTCGGAATGCATTTCGTTAGCTGTATCTTGTGGAAGGCTATGGAGTTTAATGGATGTTTTGTTGCAGAACTTGGGAGCTTACGGCGCCATGGACGGGAGTGCAGATTCAGGTACCCGTGAAATTCATTGTAGGCGATTTGGATATAACTTACCATTTTAAAGGAGCCAAGGAATATATCCACGATGGCGGGTTCAAAAGGGACGTGCCATTGCTGGAGGAAGTAGTAATAGTGAAAAATGCTGGCCACTTTGTCCATGAAGAAAAGCCACATGAAATCAATACTCACATTCATGACTTCATCAAGAAATTTTGAACAACATTTCCATTGCCAACTCATGTTAATTTCTGTTGGTGGGTCTTGAAACTTTTGATACTTTCAGCTTGTTTATCATTTTGTTAAGCCTTCGGAAAGCAAGCAGGAGCAGGAACAGTCAATTGTGTGTGTTTGGAATATTTTTCTTAATTTATAGTTAAACAATCGGA

mRNA sequence

ATGGATGCGATTCAACACACCACCATCAACACTAATGGCATCAAAATGCATATTGCCTCAATAGGCAATGGCCCCGTCGTTCTTCTTCTTCATGGCTTCCCCGAGCTGTGGTACTCATGGCGCCACCAGCTTCTCTACCTTTCCTCCGTCGGGTATCGAGCCATCGCGCCGGACCTCCGAGGCTATGGCGACACGGACTCCCCTGGATCGCATACTTCCTACACCGCCCTCCATATTGTCGGCGATTTGATTGGGGCTTTGGATGAGCTTGGGATTGAGAAGGTGTTTCTTGTAGGCCATGACTGGGGGGCGATCATTGCTTGGTACTTTTGCTTGTTCAGGCCTGAAAGAATCAAAGCCTTGGTGAATCTGAGCGTCCAGTTCTTCCCTAGAAATCCGGCTATTTCTTTCATCCAGCGTTTCAGAGCTGCGTATGGTGATGATTTCTATATGTGCAGGTTTCAGGTTCCAGGAGAGGCAGAAGCAGATTTTGCCTGCATTGACACTGTTCAACTGTTCAAGACAACACTGTCTACTAGAAGTACAGAGGCACCTTGTTTGCCTAAAGAATACGGATTTCGCGCCATCCCACCGCCAGAAAACCTTCCTTCTTGGTTGACAGAAGAAGATATCAACTATTATGCTGCCAAATTCAAGGAGACAGGCTTCACTGGAGCATTGAACTACTATCGCGCTTTTGACTTAACTTGGGAGCTTACGGCGCCATGGACGGGAGTGCAGATTCAGGTACCCGTGAAATTCATTGTAGGCGATTTGGATATAACTTACCATTTTAAAGGAGCCAAGGAATATATCCACGATGGCGGGTTCAAAAGGGACGTGCCATTGCTGGAGGAAGTAGTAATAGTGAAAAATGCTGGCCACTTTGTCCATGAAGAAAAGCCACATGAAATCAATACTCACATTCATGACTTCATCAAGAAATTTTGA

Coding sequence (CDS)

ATGGATGCGATTCAACACACCACCATCAACACTAATGGCATCAAAATGCATATTGCCTCAATAGGCAATGGCCCCGTCGTTCTTCTTCTTCATGGCTTCCCCGAGCTGTGGTACTCATGGCGCCACCAGCTTCTCTACCTTTCCTCCGTCGGGTATCGAGCCATCGCGCCGGACCTCCGAGGCTATGGCGACACGGACTCCCCTGGATCGCATACTTCCTACACCGCCCTCCATATTGTCGGCGATTTGATTGGGGCTTTGGATGAGCTTGGGATTGAGAAGGTGTTTCTTGTAGGCCATGACTGGGGGGCGATCATTGCTTGGTACTTTTGCTTGTTCAGGCCTGAAAGAATCAAAGCCTTGGTGAATCTGAGCGTCCAGTTCTTCCCTAGAAATCCGGCTATTTCTTTCATCCAGCGTTTCAGAGCTGCGTATGGTGATGATTTCTATATGTGCAGGTTTCAGGTTCCAGGAGAGGCAGAAGCAGATTTTGCCTGCATTGACACTGTTCAACTGTTCAAGACAACACTGTCTACTAGAAGTACAGAGGCACCTTGTTTGCCTAAAGAATACGGATTTCGCGCCATCCCACCGCCAGAAAACCTTCCTTCTTGGTTGACAGAAGAAGATATCAACTATTATGCTGCCAAATTCAAGGAGACAGGCTTCACTGGAGCATTGAACTACTATCGCGCTTTTGACTTAACTTGGGAGCTTACGGCGCCATGGACGGGAGTGCAGATTCAGGTACCCGTGAAATTCATTGTAGGCGATTTGGATATAACTTACCATTTTAAAGGAGCCAAGGAATATATCCACGATGGCGGGTTCAAAAGGGACGTGCCATTGCTGGAGGAAGTAGTAATAGTGAAAAATGCTGGCCACTTTGTCCATGAAGAAAAGCCACATGAAATCAATACTCACATTCATGACTTCATCAAGAAATTTTGA
BLAST of CSPI04G00960 vs. Swiss-Prot
Match: HYES_PIG (Bifunctional epoxide hydrolase 2 OS=Sus scrofa GN=EPHX2 PE=2 SV=1)

HSP 1 Score: 192.6 bits (488), Expect = 6.6e-48
Identity = 102/311 (32.80%), Postives = 171/311 (54.98%), Query Frame = 1

Query: 13  GIKMHIASIGNGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLRGYGDTDSPGSHT 72
           G+++H   +G+GP V L HGFPE W+SWR+Q+  L+  G+R +A D++GYG++ +P    
Sbjct: 247 GVRLHFVEMGSGPAVCLCHGFPESWFSWRYQIPALAQAGFRVLAVDMKGYGESSAPPEIE 306

Query: 73  SYTALHIVGDLIGALDELGIEKVFLVGHDWGAIIAWYFCLFRPERIKALVNLSVQFFPRN 132
            Y+   +  D++  L++LG+ +   +GHDWG ++ W   LF PER++A+ +L+  F P N
Sbjct: 307 EYSLEVLCKDMVTFLNKLGLSQAVFIGHDWGGVLVWNMALFYPERVRAVASLNTPFMPSN 366

Query: 133 PAISFIQRFRAAYGDDFYMCRFQVPGEAEADFA--CIDTVQLF-----KTTLSTRSTEAP 192
           P +S ++  +A    D Y   FQ PG AEA+       T + F     +T L+T      
Sbjct: 367 PNVSPMEIIKANPVFD-YQLYFQEPGVAEAELEQNLDRTFKNFFRAHDETFLTTNRV--- 426

Query: 193 CLPKEYG--FRAIPPPENLPSWLTEEDINYYAAKFKETGFTGALNYYRAFDLTWELTAPW 252
              +E G  F   P   +L   +TEEDI +Y  +FK++GF G LN+YR  +  W+     
Sbjct: 427 ---RELGGLFVGTPEEPSLSRLVTEEDIQFYVQQFKKSGFRGPLNWYRNMERNWQWGCKG 486

Query: 253 TGVQIQVPVKFIVGDLDITYHFKGAKEYIHDGGFKRDVPLLEEVVIVKNAGHFVHEEKPH 312
           +G +I +P   +  + D+  H K +K        +  +P L+   I K+ GH+   +KP 
Sbjct: 487 SGRKILIPALMVTAENDLVLHPKMSKH------MENWIPHLKRGHI-KDCGHWTQIDKPA 543

Query: 313 EINTHIHDFIK 315
           E+N  + ++++
Sbjct: 547 ELNRILIEWLE 543

BLAST of CSPI04G00960 vs. Swiss-Prot
Match: HYES_HUMAN (Bifunctional epoxide hydrolase 2 OS=Homo sapiens GN=EPHX2 PE=1 SV=2)

HSP 1 Score: 188.7 bits (478), Expect = 9.6e-47
Identity = 98/297 (33.00%), Postives = 161/297 (54.21%), Query Frame = 1

Query: 14  IKMHIASIGNGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLRGYGDTDSPGSHTS 73
           +++H   +G+GP V L HGFPE WYSWR+Q+  L+  GYR +A D++GYG++ +P     
Sbjct: 248 VRLHFVELGSGPAVCLCHGFPESWYSWRYQIPALAQAGYRVLAMDMKGYGESSAPPEIEE 307

Query: 74  YTALHIVGDLIGALDELGIEKVFLVGHDWGAIIAWYFCLFRPERIKALVNLSVQFFPRNP 133
           Y    +  +++  LD+LG+ +   +GHDWG ++ WY  LF PER++A+ +L+  F P NP
Sbjct: 308 YCMEVLCKEMVTFLDKLGLSQAVFIGHDWGGMLVWYMALFYPERVRAVASLNTPFIPANP 367

Query: 134 AISFIQRFRAAYGDDFYMCRFQVPGEAEADFACIDTVQLFKTTLSTRSTEAPCLPK--EY 193
            +S ++  +A    D Y   FQ PG AEA+    +  + FK+           + K  E 
Sbjct: 368 NMSPLESIKANPVFD-YQLYFQEPGVAEAELE-QNLSRTFKSLFRASDESVLSMHKVCEA 427

Query: 194 GFRAIPPPE--NLPSWLTEEDINYYAAKFKETGFTGALNYYRAFDLTWELTAPWTGVQIQ 253
           G   +  PE  +L   +TEE+I +Y  +FK++GF G LN+YR  +  W+      G +I 
Sbjct: 428 GGLFVNSPEEPSLSRMVTEEEIQFYVQQFKKSGFRGPLNWYRNMERNWKWACKSLGRKIL 487

Query: 254 VPVKFIVGDLDITYHFKGAKEYIHDGGFKRDVPLLEEVVIVKNAGHFVHEEKPHEIN 307
           +P   +  + D         +++ D      +P L+   I ++ GH+   +KP E+N
Sbjct: 488 IPALMVTAEKDFVL-VPQMSQHMEDW-----IPHLKRGHI-EDCGHWTQMDKPTEVN 535

BLAST of CSPI04G00960 vs. Swiss-Prot
Match: HYES_MOUSE (Bifunctional epoxide hydrolase 2 OS=Mus musculus GN=Ephx2 PE=1 SV=2)

HSP 1 Score: 186.4 bits (472), Expect = 4.8e-46
Identity = 101/298 (33.89%), Postives = 159/298 (53.36%), Query Frame = 1

Query: 13  GIKMHIASIGNGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLRGYGDTDSPGSHT 72
           GI++H   +G+GP + L HGFPE W+SWR+Q+  L+  G+R +A D++GYGD+ SP    
Sbjct: 245 GIRLHFVEMGSGPALCLCHGFPESWFSWRYQIPALAQAGFRVLAIDMKGYGDSSSPPEIE 304

Query: 73  SYTALHIVGDLIGALDELGIEKVFLVGHDWGAIIAWYFCLFRPERIKALVNLSVQFFPRN 132
            Y    +  +++  LD+LGI +   +GHDW  ++ W   LF PER++A+ +L+  F P +
Sbjct: 305 EYAMELLCKEMVTFLDKLGIPQAVFIGHDWAGVMVWNMALFYPERVRAVASLNTPFMPPD 364

Query: 133 PAISFIQRFRAAYGDDFYMCRFQVPGEAEADFA--CIDTVQLFKTTLSTRSTEAPCLPKE 192
           P +S ++  R+    + Y   FQ PG AEA+       T + F          A     E
Sbjct: 365 PDVSPMKVIRSIPVFN-YQLYFQEPGVAEAELEKNMSRTFKSFFRASDETGFIAVHKATE 424

Query: 193 YGFRAIPPPE--NLPSWLTEEDINYYAAKFKETGFTGALNYYRAFDLTWELTAPWTGVQI 252
            G   +  PE  NL    TEE+I +Y  +FK+TGF G LN+YR  +  W+ +    G +I
Sbjct: 425 IGGILVNTPEDPNLSKITTEEEIEFYIQQFKKTGFRGPLNWYRNTERNWKWSCKGLGRKI 484

Query: 253 QVPVKFIVGDLDITYHFKGAKEYIHDGGFKRDVPLLEEVVIVKNAGHFVHEEKPHEIN 307
            VP   +  + DI    + +K        ++ +P L+   I ++ GH+   EKP E+N
Sbjct: 485 LVPALMVTAEKDIVLRPEMSK------NMEKWIPFLKRGHI-EDCGHWTQIEKPTEVN 534

BLAST of CSPI04G00960 vs. Swiss-Prot
Match: HYES_RAT (Bifunctional epoxide hydrolase 2 OS=Rattus norvegicus GN=Ephx2 PE=1 SV=1)

HSP 1 Score: 183.7 bits (465), Expect = 3.1e-45
Identity = 99/306 (32.35%), Postives = 161/306 (52.61%), Query Frame = 1

Query: 13  GIKMHIASIGNGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLRGYGDTDSPGSHT 72
           GI++H   +G+GP + L HGFPE W+SWR+Q+  L+  G+R +A D++GYGD+ SP    
Sbjct: 245 GIRLHFVEMGSGPAICLCHGFPESWFSWRYQIPALAQAGFRVLAIDMKGYGDSSSPPEIE 304

Query: 73  SYTALHIVGDLIGALDELGIEKVFLVGHDWGAIIAWYFCLFRPERIKALVNLSVQFFPRN 132
            Y    +  +++  L++LGI +   +GHDW  ++ W   LF PER++A+ +L+    P N
Sbjct: 305 EYAMELLCEEMVTFLNKLGIPQAVFIGHDWAGVLVWNMALFHPERVRAVASLNTPLMPPN 364

Query: 133 PAISFIQRFRAAYGDDFYMCRFQVPGEAEADFA--CIDTVQLFKTTLSTRSTEAPCLPKE 192
           P +S ++  R+    + Y   FQ PG AEA+       T + F  T             E
Sbjct: 365 PEVSPMEVIRSIPVFN-YQLYFQEPGVAEAELEKNMSRTFKSFFRTSDDMGLLTVNKATE 424

Query: 193 YGFRAIPPPEN--LPSWLTEEDINYYAAKFKETGFTGALNYYRAFDLTWELTAPWTGVQI 252
            G   +  PE+  +    TEE+I YY  +FK++GF G LN+YR  +  W+ +    G +I
Sbjct: 425 MGGILVGTPEDPKVSKITTEEEIEYYIQQFKKSGFRGPLNWYRNTERNWKWSCKALGRKI 484

Query: 253 QVPVKFIVGDLDITYHFKGAKEYIHDGGFKRDVPLLEEVVIVKNAGHFVHEEKPHEINTH 312
            VP   +  + DI    + +K        +  +P L+   I ++ GH+   EKP E+N  
Sbjct: 485 LVPALMVTAEKDIVLRPEMSK------NMENWIPFLKRGHI-EDCGHWTQIEKPAEVNQI 542

Query: 313 IHDFIK 315
           +  ++K
Sbjct: 545 LIKWLK 542

BLAST of CSPI04G00960 vs. Swiss-Prot
Match: YFHM_BACSU (AB hydrolase superfamily protein YfhM OS=Bacillus subtilis (strain 168) GN=yfhM PE=3 SV=1)

HSP 1 Score: 154.1 bits (388), Expect = 2.6e-36
Identity = 100/319 (31.35%), Postives = 154/319 (48.28%), Query Frame = 1

Query: 1   MDAIQHTTINTNGIKMHIASIG--NGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPD 60
           MD ++   +NTNGI +H+A+ G  +GP+++LLHGFPE WY W++Q+  L   GYR IAPD
Sbjct: 1   MDGVKCQFVNTNGITLHVAAAGREDGPLIVLLHGFPEFWYGWKNQIKPLVDAGYRVIAPD 60

Query: 61  LRGYGDTDSPGSHTSYTALHIVGDLIGALDELGIEKVFLVGHDWGAIIAWYFCLFRPERI 120
            RGY  +D P    SY    +  D+IG + +   EK  ++GHDWG  +AW+    RPE +
Sbjct: 61  QRGYNLSDKPEGIDSYRIDTLRDDIIGLITQFTDEKAIVIGHDWGGAVAWHLASTRPEYL 120

Query: 121 KALVNLSVQFFPRNPAI--SFIQRFRAAYGDDFYMCRFQVPGEAEADFACIDTVQLFKTT 180
           + L+ +++     +P +  +    +   +    Y+  FQ+P   EA              
Sbjct: 121 EKLIAINIP----HPHVMKTVTPLYPPQWLKSSYIAYFQLPDIPEA-------------- 180

Query: 181 LSTRSTEAPCLPKEYGFRAIPPPENLPSWLTEEDINYYAAKFKETG-FTGALNYYRAFDL 240
            S R  +   L K  G        + P+  T ED++ Y   +K+ G  T  LN+YRA   
Sbjct: 181 -SLRENDYDTLDKAIGL------SDRPALFTSEDVSRYKEAWKQPGALTAMLNWYRALRK 240

Query: 241 TWELTAPWTGVQIQVPVKFIVGDLDITYHFKGAKEYIHDGGFKRDVPLLEEVVIVKNAGH 300
                 P       VP + I G  D     K AKE       +R  P    ++ V  A H
Sbjct: 241 GSLAEKP---SYETVPYRMIWGMEDRFLSRKLAKE------TERHCP-NGHLIFVDEASH 284

Query: 301 FVHEEKPHEINTHIHDFIK 315
           +++ EKP  +N  I +++K
Sbjct: 301 WINHEKPAIVNQLILEYLK 284

BLAST of CSPI04G00960 vs. TrEMBL
Match: A0A0A0KT70_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G001920 PE=4 SV=1)

HSP 1 Score: 669.1 bits (1725), Expect = 2.7e-189
Identity = 315/316 (99.68%), Postives = 315/316 (99.68%), Query Frame = 1

Query: 1   MDAIQHTTINTNGIKMHIASIGNGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60
           MDAIQHTTINTNGIKMHIASIGNGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR
Sbjct: 1   MDAIQHTTINTNGIKMHIASIGNGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60

Query: 61  GYGDTDSPGSHTSYTALHIVGDLIGALDELGIEKVFLVGHDWGAIIAWYFCLFRPERIKA 120
           GYGDTDSPGSHTSYTALHIVGDLIGALDELGIEKVFLVGHDWGAIIAWYFCLFRPERIKA
Sbjct: 61  GYGDTDSPGSHTSYTALHIVGDLIGALDELGIEKVFLVGHDWGAIIAWYFCLFRPERIKA 120

Query: 121 LVNLSVQFFPRNPAISFIQRFRAAYGDDFYMCRFQVPGEAEADFACIDTVQLFKTTLSTR 180
           LVNLSVQFFPRNPAISFIQRFRAAYGDDFYMCRFQVPGEAEADFACIDT QLFKTTLSTR
Sbjct: 121 LVNLSVQFFPRNPAISFIQRFRAAYGDDFYMCRFQVPGEAEADFACIDTAQLFKTTLSTR 180

Query: 181 STEAPCLPKEYGFRAIPPPENLPSWLTEEDINYYAAKFKETGFTGALNYYRAFDLTWELT 240
           STEAPCLPKEYGFRAIPPPENLPSWLTEEDINYYAAKFKETGFTGALNYYRAFDLTWELT
Sbjct: 181 STEAPCLPKEYGFRAIPPPENLPSWLTEEDINYYAAKFKETGFTGALNYYRAFDLTWELT 240

Query: 241 APWTGVQIQVPVKFIVGDLDITYHFKGAKEYIHDGGFKRDVPLLEEVVIVKNAGHFVHEE 300
           APWTGVQIQVPVKFIVGDLDITYHFKGAKEYIHDGGFKRDVPLLEEVVIVKNAGHFVHEE
Sbjct: 241 APWTGVQIQVPVKFIVGDLDITYHFKGAKEYIHDGGFKRDVPLLEEVVIVKNAGHFVHEE 300

Query: 301 KPHEINTHIHDFIKKF 317
           KPHEINTHIHDFIKKF
Sbjct: 301 KPHEINTHIHDFIKKF 316

BLAST of CSPI04G00960 vs. TrEMBL
Match: A0A0A0KY86_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G001830 PE=4 SV=1)

HSP 1 Score: 538.1 bits (1385), Expect = 7.2e-150
Identity = 242/316 (76.58%), Postives = 276/316 (87.34%), Query Frame = 1

Query: 1   MDAIQHTTINTNGIKMHIASIGNGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60
           M+ I+HTTINTNGIKMHIASIG GPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR
Sbjct: 1   METIEHTTINTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60

Query: 61  GYGDTDSPGSHTSYTALHIVGDLIGALDELGIEKVFLVGHDWGAIIAWYFCLFRPERIKA 120
           G+GDTD+P SHT+YTA HIVGDL+GALDELGIEKVF+VGHDWGAIIAWYFCLFRP+R+KA
Sbjct: 61  GFGDTDAPESHTAYTAFHIVGDLVGALDELGIEKVFVVGHDWGAIIAWYFCLFRPDRVKA 120

Query: 121 LVNLSVQFFPRNPAISFIQRFRAAYGDDFYMCRFQVPGEAEADFACIDTVQLFKTTLSTR 180
           LVNLSV F PRNPAISF+Q FR A+GDDFY+CRFQVPGEAE DF  IDT +LFK  L  R
Sbjct: 121 LVNLSVHFLPRNPAISFVQGFRFAFGDDFYICRFQVPGEAEKDFGSIDTTELFKKILCGR 180

Query: 181 STEAPCLPKEYGFRAIPPPENLPSWLTEEDINYYAAKFKETGFTGALNYYRAFDLTWELT 240
           + + P LPK  G R+IP P +LPSWL++EDINY+A KF  TGFTG  NYYRA DLTWELT
Sbjct: 181 TPDPPILPKGLGIRSIPSPHDLPSWLSQEDINYFATKFNHTGFTGGFNYYRALDLTWELT 240

Query: 241 APWTGVQIQVPVKFIVGDLDITYHFKGAKEYIHDGGFKRDVPLLEEVVIVKNAGHFVHEE 300
           A W   Q+QVPVKFIVGDLD+TY+F GAKEYIH+GGFK DVP LEEVV++++  HF+++E
Sbjct: 241 ASWNKAQVQVPVKFIVGDLDLTYYFPGAKEYIHNGGFKTDVPFLEEVVVMEDTAHFINQE 300

Query: 301 KPHEINTHIHDFIKKF 317
           +PHEI+ HIH+FIKKF
Sbjct: 301 RPHEISVHIHEFIKKF 316

BLAST of CSPI04G00960 vs. TrEMBL
Match: D8L7V8_PRUPE (Epoxide hydrolase 2 OS=Prunus persica GN=eh2 PE=2 SV=1)

HSP 1 Score: 488.0 bits (1255), Expect = 8.5e-135
Identity = 216/316 (68.35%), Postives = 262/316 (82.91%), Query Frame = 1

Query: 1   MDAIQHTTINTNGIKMHIASIGNGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60
           M+ I+HTT++TNGI MHIASIG GPVVL LHGFPELWYSWRHQLL LSS+GYR IAPDLR
Sbjct: 1   MEKIEHTTVSTNGINMHIASIGTGPVVLFLHGFPELWYSWRHQLLSLSSLGYRCIAPDLR 60

Query: 61  GYGDTDSPGSHTSYTALHIVGDLIGALDELGIEKVFLVGHDWGAIIAWYFCLFRPERIKA 120
           G+GDTD+P S  SY+ALHIVGDLIG LD LGI++VFLVGHDWGA+IAW+FCLFRP+R+KA
Sbjct: 61  GFGDTDAPPSPASYSALHIVGDLIGLLDHLGIDQVFLVGHDWGAVIAWWFCLFRPDRVKA 120

Query: 121 LVNLSVQFFPRNPAISFIQRFRAAYGDDFYMCRFQVPGEAEADFACIDTVQLFKTTLSTR 180
           LVN+SV F PRNP    +  FRA +GDD+Y+CRFQ PGE E +FA  DT  + K  L+ R
Sbjct: 121 LVNMSVAFSPRNPKRKPVDGFRALFGDDYYICRFQEPGEIEKEFAGYDTTSIMKKFLTGR 180

Query: 181 STEAPCLPKEYGFRAIPPPENLPSWLTEEDINYYAAKFKETGFTGALNYYRAFDLTWELT 240
           S + PCLPKE G RA   PE LP WL+EED+NY+A+KF +TGF G LNYYRA +LTWELT
Sbjct: 181 SPKPPCLPKELGLRAWKTPETLPPWLSEEDLNYFASKFSKTGFVGGLNYYRALNLTWELT 240

Query: 241 APWTGVQIQVPVKFIVGDLDITYHFKGAKEYIHDGGFKRDVPLLEEVVIVKNAGHFVHEE 300
            PWTG+Q++VPVKFIVGDLDITYH  G K YIH+GGFKRDVP L+EVV++++  HF+++E
Sbjct: 241 GPWTGLQVKVPVKFIVGDLDITYHIPGVKNYIHNGGFKRDVPFLQEVVVIEDGAHFINQE 300

Query: 301 KPHEINTHIHDFIKKF 317
           +P EI+ H++DFI+KF
Sbjct: 301 RPDEISRHVYDFIQKF 316

BLAST of CSPI04G00960 vs. TrEMBL
Match: A0A0D2R1J4_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_004G091300 PE=4 SV=1)

HSP 1 Score: 486.5 bits (1251), Expect = 2.5e-134
Identity = 210/316 (66.46%), Postives = 263/316 (83.23%), Query Frame = 1

Query: 1   MDAIQHTTINTNGIKMHIASIGNGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60
           M+ I+HTT+ TNGIKMH+ASIG+GP++L LHGFPELWY+WRHQLL LSS+GYR +APDLR
Sbjct: 1   MEKIEHTTVTTNGIKMHVASIGSGPIILFLHGFPELWYTWRHQLLSLSSLGYRCVAPDLR 60

Query: 61  GYGDTDSPGSHTSYTALHIVGDLIGALDELGIEKVFLVGHDWGAIIAWYFCLFRPERIKA 120
           GYGD+D+P S  SYT  HIVGDL+G LD LG++KVFLVGHDWGA+IAW FCLFRP+RIKA
Sbjct: 61  GYGDSDAPPSPESYTVFHIVGDLVGLLDALGVDKVFLVGHDWGAMIAWNFCLFRPDRIKA 120

Query: 121 LVNLSVQFFPRNPAISFIQRFRAAYGDDFYMCRFQVPGEAEADFACIDTVQLFKTTLSTR 180
           LVNLS+ + PRNP +  +  +RA +GDDFY+CRFQVPGEAEA FA +DT ++ K  L+TR
Sbjct: 121 LVNLSIPYHPRNPKVKTVDGYRALFGDDFYICRFQVPGEAEAHFAQMDTAKVMKKFLTTR 180

Query: 181 STEAPCLPKEYGFRAIPPPENLPSWLTEEDINYYAAKFKETGFTGALNYYRAFDLTWELT 240
               PC+P+E G +A+P P  LPSWL+E++INY+A KF + GFTG LNYYRA +L WEL 
Sbjct: 181 DPNPPCIPRETGLKALPDPPALPSWLSEDEINYFATKFSQKGFTGGLNYYRAMNLNWELM 240

Query: 241 APWTGVQIQVPVKFIVGDLDITYHFKGAKEYIHDGGFKRDVPLLEEVVIVKNAGHFVHEE 300
           APWTG+QIQVPVKFIVGDLDITYH  G KEY+ +GGFK++VP L+E+V+++   HF+++E
Sbjct: 241 APWTGLQIQVPVKFIVGDLDITYHIPGVKEYLQNGGFKKNVPFLQELVVMEGVAHFINQE 300

Query: 301 KPHEINTHIHDFIKKF 317
           KP EI+ HI+DFIKKF
Sbjct: 301 KPQEISMHIYDFIKKF 316

BLAST of CSPI04G00960 vs. TrEMBL
Match: A0A061DQR1_THECC (Alpha/beta-Hydrolases superfamily protein OS=Theobroma cacao GN=TCM_004689 PE=4 SV=1)

HSP 1 Score: 485.0 bits (1247), Expect = 7.2e-134
Identity = 211/316 (66.77%), Postives = 262/316 (82.91%), Query Frame = 1

Query: 1   MDAIQHTTINTNGIKMHIASIGNGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60
           M+ I+HT + TNGI MH+AS+G+GPV+L LHGFPELWYSWRHQLL LSS+GYR +APDLR
Sbjct: 1   MEKIEHTMVTTNGINMHVASVGSGPVILFLHGFPELWYSWRHQLLSLSSLGYRCVAPDLR 60

Query: 61  GYGDTDSPGSHTSYTALHIVGDLIGALDELGIEKVFLVGHDWGAIIAWYFCLFRPERIKA 120
           G+GDTD+P S  SYT  HIVGDL+G LD LG++ VFLVGHDWGA+IAWYFC FRP+R+KA
Sbjct: 61  GFGDTDAPPSAASYTVFHIVGDLVGLLDALGVDSVFLVGHDWGAVIAWYFCHFRPDRVKA 120

Query: 121 LVNLSVQFFPRNPAISFIQRFRAAYGDDFYMCRFQVPGEAEADFACIDTVQLFKTTLSTR 180
           LVN+SV + PR+ ++  ++ FRA +GDD+Y+CRFQ PGE E DFA +DT ++ K  L++R
Sbjct: 121 LVNMSVAYRPRHRSMKPVEGFRALFGDDYYICRFQEPGEIEEDFAQMDTAKIMKIFLTSR 180

Query: 181 STEAPCLPKEYGFRAIPPPENLPSWLTEEDINYYAAKFKETGFTGALNYYRAFDLTWELT 240
           +   PC+PKE GFR  P P  LPSWL+EEDINY+A+KF E GFTG LNYYRA DL+WEL 
Sbjct: 181 NPRPPCIPKEIGFRGYPDPPPLPSWLSEEDINYFASKFNEKGFTGGLNYYRALDLSWELM 240

Query: 241 APWTGVQIQVPVKFIVGDLDITYHFKGAKEYIHDGGFKRDVPLLEEVVIVKNAGHFVHEE 300
           APWTG+QI+VPVKFIVGDLDITYH  GAKEYIH+GGFK+DVP L+EVV+++   HF+++E
Sbjct: 241 APWTGLQIKVPVKFIVGDLDITYHIPGAKEYIHNGGFKKDVPFLQEVVVMEGVAHFINQE 300

Query: 301 KPHEINTHIHDFIKKF 317
           K  EI+ HI+DFIKKF
Sbjct: 301 KSEEISKHIYDFIKKF 316

BLAST of CSPI04G00960 vs. TAIR10
Match: AT4G02340.1 (AT4G02340.1 alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 468.0 bits (1203), Expect = 4.6e-132
Identity = 199/316 (62.97%), Postives = 256/316 (81.01%), Query Frame = 1

Query: 1   MDAIQHTTINTNGIKMHIASIGNGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60
           M+ I+HTTI+TNGI MH+ASIG+GPV+L +HGFP+LWYSWRHQL+  +++GYRAIAPDLR
Sbjct: 1   MEKIEHTTISTNGINMHVASIGSGPVILFVHGFPDLWYSWRHQLVSFAALGYRAIAPDLR 60

Query: 61  GYGDTDSPGSHTSYTALHIVGDLIGALDELGIEKVFLVGHDWGAIIAWYFCLFRPERIKA 120
           GYGD+D+P S  SYT LHIVGDL+G LD LG+++VFLVGHDWGAI+AW+ C+ RP+R+ A
Sbjct: 61  GYGDSDAPPSRESYTILHIVGDLVGLLDSLGVDRVFLVGHDWGAIVAWWLCMIRPDRVNA 120

Query: 121 LVNLSVQFFPRNPAISFIQRFRAAYGDDFYMCRFQVPGEAEADFACIDTVQLFKTTLSTR 180
           LVN SV F PRNP++  +  FRA +GDD+Y+CRFQ PGE E DFA +DT +L     ++R
Sbjct: 121 LVNTSVVFNPRNPSVKPVDAFRALFGDDYYICRFQEPGEIEEDFAQVDTKKLITRFFTSR 180

Query: 181 STEAPCLPKEYGFRAIPPPENLPSWLTEEDINYYAAKFKETGFTGALNYYRAFDLTWELT 240
           +   PC+PK  GFR +P P +LP+WLTE+D+ +Y  KF + GFTG LNYYRA +L+WELT
Sbjct: 181 NPRPPCIPKSVGFRGLPDPPSLPAWLTEQDVRFYGDKFSQKGFTGGLNYYRALNLSWELT 240

Query: 241 APWTGVQIQVPVKFIVGDLDITYHFKGAKEYIHDGGFKRDVPLLEEVVIVKNAGHFVHEE 300
           APWTG+QI+VPVKFIVGDLDITY+  G KEYIH+GG K+ VP L+EVV+++  GHF+H+E
Sbjct: 241 APWTGLQIKVPVKFIVGDLDITYNIPGTKEYIHEGGLKKHVPFLQEVVVMEGVGHFLHQE 300

Query: 301 KPHEINTHIHDFIKKF 317
           KP E+  HI+ F KKF
Sbjct: 301 KPDEVTDHIYGFFKKF 316

BLAST of CSPI04G00960 vs. TAIR10
Match: AT3G05600.1 (AT3G05600.1 alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 393.7 bits (1010), Expect = 1.1e-109
Identity = 179/323 (55.42%), Postives = 231/323 (71.52%), Query Frame = 1

Query: 1   MDAIQHTTINTNGIKMHIASIG--NGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPD 60
           M+ I H  ++ NGI MHIA  G   GPVVLLLHGFP+LWY+WRHQ+  LSS+GYRA+APD
Sbjct: 1   MEGIDHRMVSVNGITMHIAEKGPKEGPVVLLLHGFPDLWYTWRHQISGLSSLGYRAVAPD 60

Query: 61  LRGYGDTDSPGSHTSYTALHIVGDLIGALDELG--IEKVFLVGHDWGAIIAWYFCLFRPE 120
           LRGYGD+DSP S + YT L++VGDL+  LD +    EKVFLVGHDWGAII W+ CLFRPE
Sbjct: 61  LRGYGDSDSPESFSEYTCLNVVGDLVALLDSVAGNQEKVFLVGHDWGAIIGWFLCLFRPE 120

Query: 121 RIKALVNLSVQFFPRNPAISFIQRFRAAYGDDFYMCRFQVPGEAEADFACIDTVQLFKTT 180
           +I   V LSV +  RNP +  +Q F+A +GDD+Y+CRFQ PG+ E + A  D     +  
Sbjct: 121 KINGFVCLSVPYRSRNPKVKPVQGFKAVFGDDYYICRFQEPGKIEGEIASADPRIFLRNL 180

Query: 181 LSTRSTEAPCLPKEYGFRAIPPPEN----LPSWLTEEDINYYAAKFKETGFTGALNYYRA 240
            + R+   P LPK+  F   P P +    LP W +++D+++Y +KF++ GFTG LNYYRA
Sbjct: 181 FTGRTLGPPILPKDNPFGEKPNPNSENIELPEWFSKKDLDFYVSKFEKAGFTGGLNYYRA 240

Query: 241 FDLTWELTAPWTGVQIQVPVKFIVGDLDITYHFKGAKEYIHDGGFKRDVPLLEEVVIVKN 300
            DL WELTAPWTG +IQVPVKF+ GD D+ Y   G KEYIH GGF  DVP L+E+V++++
Sbjct: 241 MDLNWELTAPWTGAKIQVPVKFMTGDFDMVYTTPGMKEYIHGGGFAADVPTLQEIVVIED 300

Query: 301 AGHFVHEEKPHEINTHIHDFIKK 316
           AGHFV++EKP E+  HI+DF  K
Sbjct: 301 AGHFVNQEKPQEVTAHINDFFTK 323

BLAST of CSPI04G00960 vs. TAIR10
Match: AT4G15960.1 (AT4G15960.1 alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 379.0 bits (972), Expect = 2.8e-105
Identity = 173/324 (53.40%), Postives = 228/324 (70.37%), Query Frame = 1

Query: 1   MDAIQHTTINTNGIKMHIAS-----IGNGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAI 60
           +D ++H T+  NGI MH+A       G  P++L LHGFPELWY+WRHQ++ LSS+GYR I
Sbjct: 51  LDGVEHKTLKVNGINMHVAEKPGSGSGEDPIILFLHGFPELWYTWRHQMVALSSLGYRTI 110

Query: 61  APDLRGYGDTDSPGSHTSYTALHIVGDLIGALDEL--GIEKVFLVGHDWGAIIAWYFCLF 120
           APDLRGYGDT++P     YT L++ GD++  +D +  G + V +VGHDWGA+IAW  C +
Sbjct: 111 APDLRGYGDTEAPEKVEDYTYLNVDGDVVALIDAVTGGDKAVSVVGHDWGAMIAWQLCQY 170

Query: 121 RPERIKALVNLSVQFFPRNPAISFIQRFRAAYGDDFYMCRFQVPGEAEADFACIDTVQLF 180
           RPE++KALVN+SV F PRNP    +   R  +GDD+Y+CRFQ  GE E +F  + T  + 
Sbjct: 171 RPEKVKALVNMSVLFSPRNPVRVPVPTLRHVFGDDYYVCRFQKAGEIETEFKKLGTENVL 230

Query: 181 KTTLSTRSTEAPCLPKEYGF-RAIPPPENLPSWLTEEDINYYAAKFKETGFTGALNYYRA 240
           K  L+ ++     LPK+  F R+      LP WLT+ED++YY  K++  GFTG +NYYR 
Sbjct: 231 KEFLTYKTPGPLNLPKDKYFKRSENAASALPLWLTQEDLDYYVTKYENKGFTGPINYYRN 290

Query: 241 FDLTWELTAPWTGVQIQVPVKFIVGDLDITYHFKGAKEYIHDGGFKRDVPLLEEVVIVKN 300
            D  WELTAPWTG +I+VPVKFI+GD D+TY+F GAKEYI+ GGFKRDVPLL+E V++K 
Sbjct: 291 IDRNWELTAPWTGAKIRVPVKFIIGDQDLTYNFPGAKEYINGGGFKRDVPLLDETVVLKG 350

Query: 301 AGHFVHEEKPHEINTHIHDFIKKF 317
            GHF+HEE P  IN HIH+F  KF
Sbjct: 351 LGHFLHEENPDVINQHIHNFFHKF 374

BLAST of CSPI04G00960 vs. TAIR10
Match: AT2G26740.1 (AT2G26740.1 soluble epoxide hydrolase)

HSP 1 Score: 362.8 bits (930), Expect = 2.1e-100
Identity = 174/325 (53.54%), Postives = 228/325 (70.15%), Query Frame = 1

Query: 4   IQHTTINTNGIKMHIASIG--NGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLRG 63
           ++H  +  NGI +H+A  G  +GP+VLLLHGFPELWYSWRHQ+  L++ GYRA+APDLRG
Sbjct: 1   MEHRKVRGNGIDIHVAIQGPSDGPIVLLLHGFPELWYSWRHQIPGLAARGYRAVAPDLRG 60

Query: 64  YGDTDSPGSHTSYTALHIVGDLIGALDELGI---EKVFLVGHDWGAIIAWYFCLFRPERI 123
           YGD+D+P   +SYT  +IVGDLI  +  L     EKVF+VGHDWGA+IAWY CLFRP+R+
Sbjct: 61  YGDSDAPAEISSYTCFNIVGDLIAVISALTASEDEKVFVVGHDWGALIAWYLCLFRPDRV 120

Query: 124 KALVNLSVQFF--PRNPAISFIQRFRAAYGDDFYMCRFQVPGEAEADFACIDTVQLFKTT 183
           KALVNLSV F   P +P++  + R RA YGDD+Y+CRFQ  G+ EA+ A + T ++ K  
Sbjct: 121 KALVNLSVPFSFRPTDPSVKPVDRMRAFYGDDYYICRFQEFGDVEAEIAEVGTERVMKRL 180

Query: 184 LSTRSTEAPCLPKEYGF-----RAIPPPENLPSWLTEEDINYYAAKFKETGFTGALNYYR 243
           L+ R+     +PK+  F       IP    LPSWLTEED+ Y+ +KF+E GF+G +NYYR
Sbjct: 181 LTYRTPGPVIIPKDKSFWGSKGETIP----LPSWLTEEDVAYFVSKFEEKGFSGPVNYYR 240

Query: 244 AFDLTWELTAPWTGVQIQVPVKFIVGDLDITYHFKGAKEYIHDGGFKRDVPLLEEVVIVK 303
            F+   EL  PW G +IQVP KF++G+LD+ Y+  G KEYIH   FK DVPLLEE V+++
Sbjct: 241 NFNRNNELLGPWVGSKIQVPTKFVIGELDLVYYMPGVKEYIHGPQFKEDVPLLEEPVVME 300

Query: 304 NAGHFVHEEKPHEINTHIHDFIKKF 317
              HF+++EKP EI   I DFI KF
Sbjct: 301 GVAHFINQEKPQEILQIILDFISKF 321

BLAST of CSPI04G00960 vs. TAIR10
Match: AT4G15955.3 (AT4G15955.3 alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 355.9 bits (912), Expect = 2.6e-98
Identity = 170/322 (52.80%), Postives = 217/322 (67.39%), Query Frame = 1

Query: 6   HTTINTNGIKMHIAS-----IGNG----PVVLLLHGFPELWYSWRHQLLYLSSVGYRAIA 65
           H+ +  NGI MH+A       GNG    PV+L LHGFPELWY+WRHQ++ LSS+GYR IA
Sbjct: 7   HSFVKVNGITMHVAEKSPSVAGNGAIRPPVILFLHGFPELWYTWRHQMVALSSLGYRTIA 66

Query: 66  PDLRGYGDTDSPGSHTSYTALHIVGDLIGALDEL--GIEKVFLVGHDWGAIIAWYFCLFR 125
           PDLRGYGDTD+P S  +YT+LH+VGDLIG +D +    EKVF+VGHDWGAIIAW+ CLFR
Sbjct: 67  PDLRGYGDTDAPESVDAYTSLHVVGDLIGLIDAVVGDREKVFVVGHDWGAIIAWHLCLFR 126

Query: 126 PERIKALVNLSVQFFPRNPAISFIQRFRAAYGDDFYMCRFQVPGEAEADFACIDTVQLFK 185
           P+R+KALVN+SV F P NP       F+A YGDD+Y+CRFQ+         CI       
Sbjct: 127 PDRVKALVNMSVVFDPWNPKRKPTSTFKAFYGDDYYICRFQLLEILIKIHVCI------- 186

Query: 186 TTLSTRSTEAPCLPKEYGFRAIPPPENLPSWLTEEDINYYAAKFKETGFTGALNYYRAFD 245
                       + K Y         +LPSWLT+ D+ YY +K+++ GFTG +NYYR  D
Sbjct: 187 ------------VGKRYDDSV-----SLPSWLTDSDVKYYVSKYEKNGFTGPVNYYRNMD 246

Query: 246 LTWELTAPWTGVQIQVPVKFIVGDLDITYHFKGAKEYIHDGGFKRDVPLLEEVVIVKNAG 305
            TWEL    +  +++VPVKFI+GD D+TYH  G+K+YIHDG FK  VPLL+EVV++K  G
Sbjct: 247 RTWELMGSLSNAKVKVPVKFIIGDQDLTYHIPGSKKYIHDGRFKSHVPLLDEVVVIKGVG 304

Query: 306 HFVHEEKPHEINTHIHDFIKKF 317
           HF+HEE+P EI+ HIHD+   F
Sbjct: 307 HFIHEERPDEISKHIHDYFLTF 304

BLAST of CSPI04G00960 vs. NCBI nr
Match: gi|449469066|ref|XP_004152242.1| (PREDICTED: bifunctional epoxide hydrolase 2-like isoform X1 [Cucumis sativus])

HSP 1 Score: 669.1 bits (1725), Expect = 3.9e-189
Identity = 315/316 (99.68%), Postives = 315/316 (99.68%), Query Frame = 1

Query: 1   MDAIQHTTINTNGIKMHIASIGNGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60
           MDAIQHTTINTNGIKMHIASIGNGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR
Sbjct: 1   MDAIQHTTINTNGIKMHIASIGNGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60

Query: 61  GYGDTDSPGSHTSYTALHIVGDLIGALDELGIEKVFLVGHDWGAIIAWYFCLFRPERIKA 120
           GYGDTDSPGSHTSYTALHIVGDLIGALDELGIEKVFLVGHDWGAIIAWYFCLFRPERIKA
Sbjct: 61  GYGDTDSPGSHTSYTALHIVGDLIGALDELGIEKVFLVGHDWGAIIAWYFCLFRPERIKA 120

Query: 121 LVNLSVQFFPRNPAISFIQRFRAAYGDDFYMCRFQVPGEAEADFACIDTVQLFKTTLSTR 180
           LVNLSVQFFPRNPAISFIQRFRAAYGDDFYMCRFQVPGEAEADFACIDT QLFKTTLSTR
Sbjct: 121 LVNLSVQFFPRNPAISFIQRFRAAYGDDFYMCRFQVPGEAEADFACIDTAQLFKTTLSTR 180

Query: 181 STEAPCLPKEYGFRAIPPPENLPSWLTEEDINYYAAKFKETGFTGALNYYRAFDLTWELT 240
           STEAPCLPKEYGFRAIPPPENLPSWLTEEDINYYAAKFKETGFTGALNYYRAFDLTWELT
Sbjct: 181 STEAPCLPKEYGFRAIPPPENLPSWLTEEDINYYAAKFKETGFTGALNYYRAFDLTWELT 240

Query: 241 APWTGVQIQVPVKFIVGDLDITYHFKGAKEYIHDGGFKRDVPLLEEVVIVKNAGHFVHEE 300
           APWTGVQIQVPVKFIVGDLDITYHFKGAKEYIHDGGFKRDVPLLEEVVIVKNAGHFVHEE
Sbjct: 241 APWTGVQIQVPVKFIVGDLDITYHFKGAKEYIHDGGFKRDVPLLEEVVIVKNAGHFVHEE 300

Query: 301 KPHEINTHIHDFIKKF 317
           KPHEINTHIHDFIKKF
Sbjct: 301 KPHEINTHIHDFIKKF 316

BLAST of CSPI04G00960 vs. NCBI nr
Match: gi|659108663|ref|XP_008454322.1| (PREDICTED: bifunctional epoxide hydrolase 2-like isoform X1 [Cucumis melo])

HSP 1 Score: 653.3 bits (1684), Expect = 2.2e-184
Identity = 305/316 (96.52%), Postives = 311/316 (98.42%), Query Frame = 1

Query: 1   MDAIQHTTINTNGIKMHIASIGNGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60
           MDAIQHTTI TNGIKMHIAS+GNGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR
Sbjct: 1   MDAIQHTTIKTNGIKMHIASVGNGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60

Query: 61  GYGDTDSPGSHTSYTALHIVGDLIGALDELGIEKVFLVGHDWGAIIAWYFCLFRPERIKA 120
           GYGDTDSP SHTSYTALHIVGDL+GALDELGIEKVFLVGHDWGAIIAWYFCLFRPERIKA
Sbjct: 61  GYGDTDSPESHTSYTALHIVGDLVGALDELGIEKVFLVGHDWGAIIAWYFCLFRPERIKA 120

Query: 121 LVNLSVQFFPRNPAISFIQRFRAAYGDDFYMCRFQVPGEAEADFACIDTVQLFKTTLSTR 180
           LVNLSVQFFPRNPAISFIQRFRAAYGDDFYMCRFQVPGEAEADFACIDT QLFKTTLS R
Sbjct: 121 LVNLSVQFFPRNPAISFIQRFRAAYGDDFYMCRFQVPGEAEADFACIDTAQLFKTTLSNR 180

Query: 181 STEAPCLPKEYGFRAIPPPENLPSWLTEEDINYYAAKFKETGFTGALNYYRAFDLTWELT 240
           ST+APCLPKEYGFRAIPPPENLPSWLTEEDINYYAAKFKETGFTGALNYYRAFDLTWELT
Sbjct: 181 STKAPCLPKEYGFRAIPPPENLPSWLTEEDINYYAAKFKETGFTGALNYYRAFDLTWELT 240

Query: 241 APWTGVQIQVPVKFIVGDLDITYHFKGAKEYIHDGGFKRDVPLLEEVVIVKNAGHFVHEE 300
           APWTGVQIQVPVKFIVGD D+TYHFKGAKEYIH+GGFKRDVPLLEEVVIV+NAGHFVHEE
Sbjct: 241 APWTGVQIQVPVKFIVGDSDLTYHFKGAKEYIHEGGFKRDVPLLEEVVIVENAGHFVHEE 300

Query: 301 KPHEINTHIHDFIKKF 317
           KPHEINTHIHDFIKKF
Sbjct: 301 KPHEINTHIHDFIKKF 316

BLAST of CSPI04G00960 vs. NCBI nr
Match: gi|659108677|ref|XP_008454330.1| (PREDICTED: bifunctional epoxide hydrolase 2-like [Cucumis melo])

HSP 1 Score: 539.3 bits (1388), Expect = 4.6e-150
Identity = 243/316 (76.90%), Postives = 274/316 (86.71%), Query Frame = 1

Query: 1   MDAIQHTTINTNGIKMHIASIGNGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60
           M+AI+HTTINTNGI MHIASIG GPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR
Sbjct: 1   MEAIEHTTINTNGINMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60

Query: 61  GYGDTDSPGSHTSYTALHIVGDLIGALDELGIEKVFLVGHDWGAIIAWYFCLFRPERIKA 120
           G+GDTD+P SHT YT  HIVGDL+GALDELGIEKVF+VGHDWGAIIAWY CLFRP+R+KA
Sbjct: 61  GFGDTDAPESHTVYTPFHIVGDLVGALDELGIEKVFVVGHDWGAIIAWYLCLFRPDRVKA 120

Query: 121 LVNLSVQFFPRNPAISFIQRFRAAYGDDFYMCRFQVPGEAEADFACIDTVQLFKTTLSTR 180
           LVNLSV F  RNP ISF+Q FR A+GDDFY+CRFQVPGEAE DF  IDT +LFK  L  R
Sbjct: 121 LVNLSVHFLRRNPTISFVQGFRIAFGDDFYICRFQVPGEAEKDFGSIDTAELFKKFLCGR 180

Query: 181 STEAPCLPKEYGFRAIPPPENLPSWLTEEDINYYAAKFKETGFTGALNYYRAFDLTWELT 240
           S + P LPKE+G R+IP P+NLPSWL++EDINY+A KF  TGFTG  NYYRA DLTWELT
Sbjct: 181 SPDPPILPKEFGIRSIPSPQNLPSWLSQEDINYFATKFNRTGFTGGFNYYRALDLTWELT 240

Query: 241 APWTGVQIQVPVKFIVGDLDITYHFKGAKEYIHDGGFKRDVPLLEEVVIVKNAGHFVHEE 300
           A W   Q+QVPVKFIVGDLD+TYHF GAKEYIH+GGFK DVP LEEVV++++  HF+++E
Sbjct: 241 ASWNKAQVQVPVKFIVGDLDLTYHFPGAKEYIHNGGFKTDVPFLEEVVVMEDTAHFINQE 300

Query: 301 KPHEINTHIHDFIKKF 317
           +PHEI+ HIHDFIKKF
Sbjct: 301 RPHEISAHIHDFIKKF 316

BLAST of CSPI04G00960 vs. NCBI nr
Match: gi|449469072|ref|XP_004152245.1| (PREDICTED: bifunctional epoxide hydrolase 2-like [Cucumis sativus])

HSP 1 Score: 538.1 bits (1385), Expect = 1.0e-149
Identity = 242/316 (76.58%), Postives = 276/316 (87.34%), Query Frame = 1

Query: 1   MDAIQHTTINTNGIKMHIASIGNGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60
           M+ I+HTTINTNGIKMHIASIG GPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR
Sbjct: 1   METIEHTTINTNGIKMHIASIGTGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60

Query: 61  GYGDTDSPGSHTSYTALHIVGDLIGALDELGIEKVFLVGHDWGAIIAWYFCLFRPERIKA 120
           G+GDTD+P SHT+YTA HIVGDL+GALDELGIEKVF+VGHDWGAIIAWYFCLFRP+R+KA
Sbjct: 61  GFGDTDAPESHTAYTAFHIVGDLVGALDELGIEKVFVVGHDWGAIIAWYFCLFRPDRVKA 120

Query: 121 LVNLSVQFFPRNPAISFIQRFRAAYGDDFYMCRFQVPGEAEADFACIDTVQLFKTTLSTR 180
           LVNLSV F PRNPAISF+Q FR A+GDDFY+CRFQVPGEAE DF  IDT +LFK  L  R
Sbjct: 121 LVNLSVHFLPRNPAISFVQGFRFAFGDDFYICRFQVPGEAEKDFGSIDTTELFKKILCGR 180

Query: 181 STEAPCLPKEYGFRAIPPPENLPSWLTEEDINYYAAKFKETGFTGALNYYRAFDLTWELT 240
           + + P LPK  G R+IP P +LPSWL++EDINY+A KF  TGFTG  NYYRA DLTWELT
Sbjct: 181 TPDPPILPKGLGIRSIPSPHDLPSWLSQEDINYFATKFNHTGFTGGFNYYRALDLTWELT 240

Query: 241 APWTGVQIQVPVKFIVGDLDITYHFKGAKEYIHDGGFKRDVPLLEEVVIVKNAGHFVHEE 300
           A W   Q+QVPVKFIVGDLD+TY+F GAKEYIH+GGFK DVP LEEVV++++  HF+++E
Sbjct: 241 ASWNKAQVQVPVKFIVGDLDLTYYFPGAKEYIHNGGFKTDVPFLEEVVVMEDTAHFINQE 300

Query: 301 KPHEINTHIHDFIKKF 317
           +PHEI+ HIH+FIKKF
Sbjct: 301 RPHEISVHIHEFIKKF 316

BLAST of CSPI04G00960 vs. NCBI nr
Match: gi|778689338|ref|XP_011652941.1| (PREDICTED: bifunctional epoxide hydrolase 2-like isoform X2 [Cucumis sativus])

HSP 1 Score: 494.6 bits (1272), Expect = 1.3e-136
Identity = 233/234 (99.57%), Postives = 233/234 (99.57%), Query Frame = 1

Query: 1   MDAIQHTTINTNGIKMHIASIGNGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60
           MDAIQHTTINTNGIKMHIASIGNGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR
Sbjct: 1   MDAIQHTTINTNGIKMHIASIGNGPVVLLLHGFPELWYSWRHQLLYLSSVGYRAIAPDLR 60

Query: 61  GYGDTDSPGSHTSYTALHIVGDLIGALDELGIEKVFLVGHDWGAIIAWYFCLFRPERIKA 120
           GYGDTDSPGSHTSYTALHIVGDLIGALDELGIEKVFLVGHDWGAIIAWYFCLFRPERIKA
Sbjct: 61  GYGDTDSPGSHTSYTALHIVGDLIGALDELGIEKVFLVGHDWGAIIAWYFCLFRPERIKA 120

Query: 121 LVNLSVQFFPRNPAISFIQRFRAAYGDDFYMCRFQVPGEAEADFACIDTVQLFKTTLSTR 180
           LVNLSVQFFPRNPAISFIQRFRAAYGDDFYMCRFQVPGEAEADFACIDT QLFKTTLSTR
Sbjct: 121 LVNLSVQFFPRNPAISFIQRFRAAYGDDFYMCRFQVPGEAEADFACIDTAQLFKTTLSTR 180

Query: 181 STEAPCLPKEYGFRAIPPPENLPSWLTEEDINYYAAKFKETGFTGALNYYRAFD 235
           STEAPCLPKEYGFRAIPPPENLPSWLTEEDINYYAAKFKETGFTGALNYYRAFD
Sbjct: 181 STEAPCLPKEYGFRAIPPPENLPSWLTEEDINYYAAKFKETGFTGALNYYRAFD 234

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
HYES_PIG6.6e-4832.80Bifunctional epoxide hydrolase 2 OS=Sus scrofa GN=EPHX2 PE=2 SV=1[more]
HYES_HUMAN9.6e-4733.00Bifunctional epoxide hydrolase 2 OS=Homo sapiens GN=EPHX2 PE=1 SV=2[more]
HYES_MOUSE4.8e-4633.89Bifunctional epoxide hydrolase 2 OS=Mus musculus GN=Ephx2 PE=1 SV=2[more]
HYES_RAT3.1e-4532.35Bifunctional epoxide hydrolase 2 OS=Rattus norvegicus GN=Ephx2 PE=1 SV=1[more]
YFHM_BACSU2.6e-3631.35AB hydrolase superfamily protein YfhM OS=Bacillus subtilis (strain 168) GN=yfhM ... [more]
Match NameE-valueIdentityDescription
A0A0A0KT70_CUCSA2.7e-18999.68Uncharacterized protein OS=Cucumis sativus GN=Csa_4G001920 PE=4 SV=1[more]
A0A0A0KY86_CUCSA7.2e-15076.58Uncharacterized protein OS=Cucumis sativus GN=Csa_4G001830 PE=4 SV=1[more]
D8L7V8_PRUPE8.5e-13568.35Epoxide hydrolase 2 OS=Prunus persica GN=eh2 PE=2 SV=1[more]
A0A0D2R1J4_GOSRA2.5e-13466.46Uncharacterized protein OS=Gossypium raimondii GN=B456_004G091300 PE=4 SV=1[more]
A0A061DQR1_THECC7.2e-13466.77Alpha/beta-Hydrolases superfamily protein OS=Theobroma cacao GN=TCM_004689 PE=4 ... [more]
Match NameE-valueIdentityDescription
AT4G02340.14.6e-13262.97 alpha/beta-Hydrolases superfamily protein[more]
AT3G05600.11.1e-10955.42 alpha/beta-Hydrolases superfamily protein[more]
AT4G15960.12.8e-10553.40 alpha/beta-Hydrolases superfamily protein[more]
AT2G26740.12.1e-10053.54 soluble epoxide hydrolase[more]
AT4G15955.32.6e-9852.80 alpha/beta-Hydrolases superfamily protein[more]
Match NameE-valueIdentityDescription
gi|449469066|ref|XP_004152242.1|3.9e-18999.68PREDICTED: bifunctional epoxide hydrolase 2-like isoform X1 [Cucumis sativus][more]
gi|659108663|ref|XP_008454322.1|2.2e-18496.52PREDICTED: bifunctional epoxide hydrolase 2-like isoform X1 [Cucumis melo][more]
gi|659108677|ref|XP_008454330.1|4.6e-15076.90PREDICTED: bifunctional epoxide hydrolase 2-like [Cucumis melo][more]
gi|449469072|ref|XP_004152245.1|1.0e-14976.58PREDICTED: bifunctional epoxide hydrolase 2-like [Cucumis sativus][more]
gi|778689338|ref|XP_011652941.1|1.3e-13699.57PREDICTED: bifunctional epoxide hydrolase 2-like isoform X2 [Cucumis sativus][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000073AB_hydrolase_1
IPR000639Epox_hydrolase-like
Vocabulary: Molecular Function
TermDefinition
GO:0003824catalytic activity
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0018108 peptidyl-tyrosine phosphorylation
biological_process GO:0008152 metabolic process
cellular_component GO:0005777 peroxisome
molecular_function GO:0016787 hydrolase activity
molecular_function GO:0004715 non-membrane spanning protein tyrosine kinase activity
molecular_function GO:0003824 catalytic activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI04G00960.1CSPI04G00960.1mRNA


Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000073Alpha/beta hydrolase fold-1PRINTSPR00111ABHYDROLASEcoord: 97..110
score: 6.3E-7coord: 111..124
score: 6.3E-7coord: 51..66
score: 6.
IPR000073Alpha/beta hydrolase fold-1PFAMPF12697Abhydrolase_6coord: 27..305
score: 2.5
IPR000639Epoxide hydrolase-likePRINTSPR00412EPOXHYDRLASEcoord: 111..124
score: 2.1E-44coord: 51..66
score: 2.1E-44coord: 97..110
score: 2.1E-44coord: 290..312
score: 2.1E-44coord: 31..49
score: 2.1E-44coord: 250..266
score: 2.1
NoneNo IPR availablePANTHERPTHR10992ALPHA/BETA HYDROLASE FOLD-CONTAINING PROTEINcoord: 200..315
score: 6.9E-175coord: 4..165
score: 6.9E
NoneNo IPR availablePANTHERPTHR10992:SF811ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEINcoord: 200..315
score: 6.9E-175coord: 4..165
score: 6.9E

The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
CSPI04G00960Cucsa.096980Cucumber (Gy14) v1cgycpiB103
The following gene(s) are paralogous to this gene:

None