CSPI04G00950 (gene) Wild cucumber (PI 183967) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CSPI04G00950
Type	gene
Organism	Cucumis sativus (Wild cucumber (PI 183967))
Description	Epoxide hydrolase 2
Location	Chr4 : 494674 .. 496029 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GAAATTGAAAGGGTGTGGAAGTGAATGAATTAGGTAAGGCTCCTGCATGCAAAATTGCTTCCATTATTTAAACCCACATAGAGAATAAACAAAACAAAGCCATCAATGGAGAAAATCCAACACTCAACCATCCCTACCAATGGCATTAACATCCACCTTGCTTCCATTGGCTCCGGCCCCGCGGTGCTATTCCTCCATGGCTTCCCAGAGATCTGGTACACATGGCGTCACCAGCTTCTCTTTTTTGCTTCCAAGGGCTTCCGAGCCATAGCGCCTGATCTCCGTGGATATGGTGACTCCGATGTGCCACCATCTCCTTCTTCCTATACAGCTCACCACATCGTCGGCGATCTCATTGGCCTCCTCGACCATCTCGAAATCGATCAAGTTTTCTTAGTAGGCCACGACTTGGGAGCTACTATAGCTTGGTACTTTTCCATTTTCAGGCCTGATCGAGTCAAGGCTTTGGTCAATTTGAGTGTTTATCATACTCCTCGGATTCCGGAAATGCCGCCTCTCGCCATTATCCGTTTGTTCCTTGGTGATGATTTCTACATTTGTAAATTTCAGGTGAAGGCGATTGAGTCTTTGAACTCTAGCACAAATTCAATTGGGATACATATATCTGATGAGTTTCAATCACATGCATTAATTAAATGTACTGGTTAATAATGAACAGAAAATTGTGAATTTTGAAACAGGAACCTGGAGTCGCAGAAGCAGATTTAGGGTCAGTAGATACGGCTACGATGATGAAGAAATTCCTGACGTTGAGAGATCCAAGCGCTCCGATTGCACCAAACGGATTCAGTACACTACTGGCAACTCCAGAAACCTTACCTTCGTGGTTAACAGAGGAAGATGTAGATTATTACGCTTCCAAATTCGCGAAAACCGGCTTCACCGGCGGACTAAACTACTATCGTGCTCTTGACCTGTGTGGATAGTAACATATTATATTCTTCGTATTTTGAATCAATTGGATTATCTAATTAGAAACCGATGTCGTTTGTGTGCGTGTGTTTTGTTGATTGCTTGTAGAACTTGGGAGCTGACGGGGCCATGGACTGGAGCAAAGATCAAAGTTCCAACAAAATTCATTGTTGGGGATCAAGATCTGGTATACCATTTTCCGGGGGCGAAGGAGTACATTCACGGCGACAGTTTCAAGGAGGACGTACCGCTTTTAGAAGAAGTGGTGGTGATAGAAGGAGCAGCTCACTTCATTAACCAAGAGAAGGCCGATGAAATAAATTCCCTCATCTACGATTTCATCACCAAATTCTAAATAATTCTCACATGGCACACCCCACTACTACTGCTGCACTTACCTCATATGGTCTTATATTTTAGCAC

mRNA sequence

ATGGAGAAAATCCAACACTCAACCATCCCTACCAATGGCATTAACATCCACCTTGCTTCCATTGGCTCCGGCCCCGCGGTGCTATTCCTCCATGGCTTCCCAGAGATCTGGTACACATGGCGTCACCAGCTTCTCTTTTTTGCTTCCAAGGGCTTCCGAGCCATAGCGCCTGATCTCCGTGGATATGGTGACTCCGATGTGCCACCATCTCCTTCTTCCTATACAGCTCACCACATCGTCGGCGATCTCATTGGCCTCCTCGACCATCTCGAAATCGATCAAGTTTTCTTAGTAGGCCACGACTTGGGAGCTACTATAGCTTGGTACTTTTCCATTTTCAGGCCTGATCGAGTCAAGGCTTTGGTCAATTTGAGTGTTTATCATACTCCTCGGATTCCGGAAATGCCGCCTCTCGCCATTATCCGTTTGTTCCTTGGTGATGATTTCTACATTTGTAAATTTCAGGAACCTGGAGTCGCAGAAGCAGATTTAGGGTCAGTAGATACGGCTACGATGATGAAGAAATTCCTGACGTTGAGAGATCCAAGCGCTCCGATTGCACCAAACGGATTCAGTACACTACTGGCAACTCCAGAAACCTTACCTTCGTGGTTAACAGAGGAAGATGTAGATTATTACGCTTCCAAATTCGCGAAAACCGGCTTCACCGGCGGACTAAACTACTATCGTGCTCTTGACCTAACTTGGGAGCTGACGGGGCCATGGACTGGAGCAAAGATCAAAGTTCCAACAAAATTCATTGTTGGGGATCAAGATCTGGTATACCATTTTCCGGGGGCGAAGGAGTACATTCACGGCGACAGTTTCAAGGAGGACGTACCGCTTTTAGAAGAAGTGGTGGTGATAGAAGGAGCAGCTCACTTCATTAACCAAGAGAAGGCCGATGAAATAAATTCCCTCATCTACGATTTCATCACCAAATTCTAA

Coding sequence (CDS)

ATGGAGAAAATCCAACACTCAACCATCCCTACCAATGGCATTAACATCCACCTTGCTTCCATTGGCTCCGGCCCCGCGGTGCTATTCCTCCATGGCTTCCCAGAGATCTGGTACACATGGCGTCACCAGCTTCTCTTTTTTGCTTCCAAGGGCTTCCGAGCCATAGCGCCTGATCTCCGTGGATATGGTGACTCCGATGTGCCACCATCTCCTTCTTCCTATACAGCTCACCACATCGTCGGCGATCTCATTGGCCTCCTCGACCATCTCGAAATCGATCAAGTTTTCTTAGTAGGCCACGACTTGGGAGCTACTATAGCTTGGTACTTTTCCATTTTCAGGCCTGATCGAGTCAAGGCTTTGGTCAATTTGAGTGTTTATCATACTCCTCGGATTCCGGAAATGCCGCCTCTCGCCATTATCCGTTTGTTCCTTGGTGATGATTTCTACATTTGTAAATTTCAGGAACCTGGAGTCGCAGAAGCAGATTTAGGGTCAGTAGATACGGCTACGATGATGAAGAAATTCCTGACGTTGAGAGATCCAAGCGCTCCGATTGCACCAAACGGATTCAGTACACTACTGGCAACTCCAGAAACCTTACCTTCGTGGTTAACAGAGGAAGATGTAGATTATTACGCTTCCAAATTCGCGAAAACCGGCTTCACCGGCGGACTAAACTACTATCGTGCTCTTGACCTAACTTGGGAGCTGACGGGGCCATGGACTGGAGCAAAGATCAAAGTTCCAACAAAATTCATTGTTGGGGATCAAGATCTGGTATACCATTTTCCGGGGGCGAAGGAGTACATTCACGGCGACAGTTTCAAGGAGGACGTACCGCTTTTAGAAGAAGTGGTGGTGATAGAAGGAGCAGCTCACTTCATTAACCAAGAGAAGGCCGATGAAATAAATTCCCTCATCTACGATTTCATCACCAAATTCTAA

Homology

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BLAST of CSPI04G00950 vs. Swiss-Prot
Match: HYES_MOUSE (Bifunctional epoxide hydrolase 2 OS=Mus musculus GN=Ephx2 PE=1 SV=2)

HSP 1 Score: 192.2 bits (487), Expect = 8.6e-48
Identity = 103/306 (33.66%), Postives = 165/306 (53.92%), Query Frame = 1


Query: 13  GINIHLASIGSGPAVLFLHGFPEIWYTWRHQLLFFASKGFRAIAPDLRGYGDSDVPPSPS 72
           GI +H   +GSGPA+   HGFPE W++WR+Q+   A  GFR +A D++GYGDS  PP   
Sbjct: 245 GIRLHFVEMGSGPALCLCHGFPESWFSWRYQIPALAQAGFRVLAIDMKGYGDSSSPPEIE 304

Query: 73  SYTAHHIVGDLIGLLDHLEIDQVFLVGHDLGATIAWYFSIFRPDRVKALVNLSVYHTPRI 132
            Y    +  +++  LD L I Q   +GHD    + W  ++F P+RV+A+ +L+    P  
Sbjct: 305 EYAMELLCKEMVTFLDKLGIPQAVFIGHDWAGVMVWNMALFYPERVRAVASLNTPFMPPD 364

Query: 133 PEMPPLAIIRLFLGDDFYICKFQEPGVAEADLGSVDTATMMKKFLTLRDPSAPIAPNGFS 192
           P++ P+ +IR     ++ +  FQEPGVAEA+L   + +   K F    D +  IA +  +
Sbjct: 365 PDVSPMKVIRSIPVFNYQL-YFQEPGVAEAEL-EKNMSRTFKSFFRASDETGFIAVHKAT 424

Query: 193 ----TLLATPE--TLPSWLTEEDVDYYASKFAKTGFTGGLNYYRALDLTWELTGPWTGAK 252
                L+ TPE   L    TEE++++Y  +F KTGF G LN+YR  +  W+ +    G K
Sbjct: 425 EIGGILVNTPEDPNLSKITTEEEIEFYIQQFKKTGFRGPLNWYRNTERNWKWSCKGLGRK 484

Query: 253 IKVPTKFIVGDQDLVYHFPGAKEYIHGDSFKEDVPLLEEVVVIEGAAHFINQEKADEINS 312
           I VP   +  ++D+V     +K      + ++ +P L+    IE   H+   EK  E+N 
Sbjct: 485 ILVPALMVTAEKDIVLRPEMSK------NMEKWIPFLKR-GHIEDCGHWTQIEKPTEVNQ 541

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BLAST of CSPI04G00950 vs. Swiss-Prot
Match: HYES_PIG (Bifunctional epoxide hydrolase 2 OS=Sus scrofa GN=EPHX2 PE=2 SV=1)

HSP 1 Score: 191.8 bits (486), Expect = 1.1e-47
Identity = 102/306 (33.33%), Postives = 167/306 (54.58%), Query Frame = 1


Query: 13  GINIHLASIGSGPAVLFLHGFPEIWYTWRHQLLFFASKGFRAIAPDLRGYGDSDVPPSPS 72
           G+ +H   +GSGPAV   HGFPE W++WR+Q+   A  GFR +A D++GYG+S  PP   
Sbjct: 247 GVRLHFVEMGSGPAVCLCHGFPESWFSWRYQIPALAQAGFRVLAVDMKGYGESSAPPEIE 306

Query: 73  SYTAHHIVGDLIGLLDHLEIDQVFLVGHDLGATIAWYFSIFRPDRVKALVNLSVYHTPRI 132
            Y+   +  D++  L+ L + Q   +GHD G  + W  ++F P+RV+A+ +L+    P  
Sbjct: 307 EYSLEVLCKDMVTFLNKLGLSQAVFIGHDWGGVLVWNMALFYPERVRAVASLNTPFMPSN 366

Query: 133 PEMPPLAIIRLFLGDDFYICKFQEPGVAEADLGSVDTATMMKKFLTLRDPSAPIAPNGFS 192
           P + P+ II+     D+ +  FQEPGVAEA+L   +     K F    D +  +  N   
Sbjct: 367 PNVSPMEIIKANPVFDYQL-YFQEPGVAEAEL-EQNLDRTFKNFFRAHDETF-LTTNRVR 426

Query: 193 TL----LATPE--TLPSWLTEEDVDYYASKFAKTGFTGGLNYYRALDLTWELTGPWTGAK 252
            L    + TPE  +L   +TEED+ +Y  +F K+GF G LN+YR ++  W+     +G K
Sbjct: 427 ELGGLFVGTPEEPSLSRLVTEEDIQFYVQQFKKSGFRGPLNWYRNMERNWQWGCKGSGRK 486

Query: 253 IKVPTKFIVGDQDLVYHFPGAKEYIHGDSFKEDVPLLEEVVVIEGAAHFINQEKADEINS 312
           I +P   +  + DLV H   +K   H +++   +P L+    I+   H+   +K  E+N 
Sbjct: 487 ILIPALMVTAENDLVLHPKMSK---HMENW---IPHLKR-GHIKDCGHWTQIDKPAELNR 542

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BLAST of CSPI04G00950 vs. Swiss-Prot
Match: HYES_RAT (Bifunctional epoxide hydrolase 2 OS=Rattus norvegicus GN=Ephx2 PE=1 SV=1)

HSP 1 Score: 189.9 bits (481), Expect = 4.3e-47
Identity = 101/306 (33.01%), Postives = 162/306 (52.94%), Query Frame = 1


Query: 13  GINIHLASIGSGPAVLFLHGFPEIWYTWRHQLLFFASKGFRAIAPDLRGYGDSDVPPSPS 72
           GI +H   +GSGPA+   HGFPE W++WR+Q+   A  GFR +A D++GYGDS  PP   
Sbjct: 245 GIRLHFVEMGSGPAICLCHGFPESWFSWRYQIPALAQAGFRVLAIDMKGYGDSSSPPEIE 304

Query: 73  SYTAHHIVGDLIGLLDHLEIDQVFLVGHDLGATIAWYFSIFRPDRVKALVNLSVYHTPRI 132
            Y    +  +++  L+ L I Q   +GHD    + W  ++F P+RV+A+ +L+    P  
Sbjct: 305 EYAMELLCEEMVTFLNKLGIPQAVFIGHDWAGVLVWNMALFHPERVRAVASLNTPLMPPN 364

Query: 133 PEMPPLAIIRLFLGDDFYICKFQEPGVAEADLGSVDTATMMKKFLTLRDPSAPIAPNGFS 192
           PE+ P+ +IR     ++ +  FQEPGVAEA+L   + +   K F    D    +  N  +
Sbjct: 365 PEVSPMEVIRSIPVFNYQL-YFQEPGVAEAEL-EKNMSRTFKSFFRTSDDMGLLTVNKAT 424

Query: 193 ----TLLATPE--TLPSWLTEEDVDYYASKFAKTGFTGGLNYYRALDLTWELTGPWTGAK 252
                L+ TPE   +    TEE+++YY  +F K+GF G LN+YR  +  W+ +    G K
Sbjct: 425 EMGGILVGTPEDPKVSKITTEEEIEYYIQQFKKSGFRGPLNWYRNTERNWKWSCKALGRK 484

Query: 253 IKVPTKFIVGDQDLVYHFPGAKEYIHGDSFKEDVPLLEEVVVIEGAAHFINQEKADEINS 312
           I VP   +  ++D+V     +K      + +  +P L+    IE   H+   EK  E+N 
Sbjct: 485 ILVPALMVTAEKDIVLRPEMSK------NMENWIPFLKR-GHIEDCGHWTQIEKPAEVNQ 541

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BLAST of CSPI04G00950 vs. Swiss-Prot
Match: HYES_HUMAN (Bifunctional epoxide hydrolase 2 OS=Homo sapiens GN=EPHX2 PE=1 SV=2)

HSP 1 Score: 186.0 bits (471), Expect = 6.2e-46
Identity = 98/305 (32.13%), Postives = 161/305 (52.79%), Query Frame = 1


Query: 14  INIHLASIGSGPAVLFLHGFPEIWYTWRHQLLFFASKGFRAIAPDLRGYGDSDVPPSPSS 73
           + +H   +GSGPAV   HGFPE WY+WR+Q+   A  G+R +A D++GYG+S  PP    
Sbjct: 248 VRLHFVELGSGPAVCLCHGFPESWYSWRYQIPALAQAGYRVLAMDMKGYGESSAPPEIEE 307

Query: 74  YTAHHIVGDLIGLLDHLEIDQVFLVGHDLGATIAWYFSIFRPDRVKALVNLSVYHTPRIP 133
           Y    +  +++  LD L + Q   +GHD G  + WY ++F P+RV+A+ +L+    P  P
Sbjct: 308 YCMEVLCKEMVTFLDKLGLSQAVFIGHDWGGMLVWYMALFYPERVRAVASLNTPFIPANP 367

Query: 134 EMPPLAIIRLFLGDDFYICKFQEPGVAEADLGSVDTATMMKKFLTLRDPSA----PIAPN 193
            M PL  I+     D+ +  FQEPGVAEA+L   + +   K      D S      +   
Sbjct: 368 NMSPLESIKANPVFDYQL-YFQEPGVAEAEL-EQNLSRTFKSLFRASDESVLSMHKVCEA 427

Query: 194 GFSTLLATPE--TLPSWLTEEDVDYYASKFAKTGFTGGLNYYRALDLTWELTGPWTGAKI 253
           G    + +PE  +L   +TEE++ +Y  +F K+GF G LN+YR ++  W+      G KI
Sbjct: 428 G-GLFVNSPEEPSLSRMVTEEEIQFYVQQFKKSGFRGPLNWYRNMERNWKWACKSLGRKI 487

Query: 254 KVPTKFIVGDQDLVYHFPGAKEYIHGDSFKEDVPLLEEVVVIEGAAHFINQEKADEINSL 313
            +P   +  ++D V   P   +++     ++ +P L+    IE   H+   +K  E+N +
Sbjct: 488 LIPALMVTAEKDFVL-VPQMSQHM-----EDWIPHLKR-GHIEDCGHWTQMDKPTEVNQI 542

[Close]

BLAST of CSPI04G00950 vs. Swiss-Prot
Match: EPHX3_HUMAN (Epoxide hydrolase 3 OS=Homo sapiens GN=EPHX3 PE=2 SV=1)

HSP 1 Score: 131.3 bits (329), Expect = 1.8e-29
Identity = 97/307 (31.60%), Postives = 148/307 (48.21%), Query Frame = 1


Query: 9   IPTNGINIHLASIG--SGPAVLFLHGFPEIWYTWRHQLLFFASKGFRAIAPDLRGYGDSD 68
           + ++G+ +H  S G  +GP +LFLHGFPE W++WR+QL  F S+ F  +A DLRGYG SD
Sbjct: 80  LKSSGLRLHYVSAGRGNGPLMLFLHGFPENWFSWRYQLREFQSR-FHVVAVDLRGYGPSD 139

Query: 69  VPPSPSSYTAHHIVGDLIGLLDHLEIDQVFLVGHDLGATIAWYFSIFRPDRVKALVNLSV 128
            P     YT   ++ D+  ++  L   +  LV HD GA +AW+FSI+ P  V+ +V +S 
Sbjct: 140 APRDVDCYTIDLLLVDIKDVILGLGYSKCILVAHDWGALLAWHFSIYYPSLVERMVVVSG 199

Query: 129 YHTPRIPEMPPLAIIRLFLGDDFYICKFQEPGVAEADLGSVDTATMMKKFLTLRDPSAPI 188
                  +     I + F     Y+  FQ P + E  L S+    ++K  LT R    P 
Sbjct: 200 APMSVYQDYSLHHISQFF--RSHYMFLFQLPWLPE-KLLSMSDFQILKTTLTHRKTGIPC 259

Query: 189 APNGFSTLLATPETLPSWLTEEDVDYYASKFAKT-GFTGGLNYYRALDLTWELTGPWTGA 248
                             LT  +++ +   F++  G TG LNYYR L   +    P    
Sbjct: 260 ------------------LTPSELEAFLYNFSQPGGLTGPLNYYRNLFRNF----PLEPQ 319

Query: 249 KIKVPTKFIVGDQDLVYHFPGAKEYIHGDSFKEDVPLLEEVVVIEGAAHFINQEKADEIN 308
           ++  PT  + G++D  Y   G  E I G  F   VP   E  ++ G  H+I Q    E++
Sbjct: 320 ELTTPTLLLWGEKD-TYLELGLVEAI-GSRF---VPGRLEAHILPGIGHWIPQSNPQEMH 355

Query: 309 SLIYDFI 313
             ++ F+
Sbjct: 380 QYMWAFL 355

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BLAST of CSPI04G00950 vs. TrEMBL
Match: A0A0A0KWK9_CUCSA (Epoxide hydrolase 2 OS=Cucumis sativus GN=Csa_4G001890 PE=4 SV=1)

HSP 1 Score: 654.4 bits (1687), Expect = 6.8e-185
Identity = 311/315 (98.73%), Postives = 314/315 (99.68%), Query Frame = 1


Query: 1   MEKIQHSTIPTNGINIHLASIGSGPAVLFLHGFPEIWYTWRHQLLFFASKGFRAIAPDLR 60
           MEKIQHSTIPTNGINIHLASIGSGPA+LFLHGFPEIWYTWRHQLLFFAS+GFRAIAPDLR
Sbjct: 1   MEKIQHSTIPTNGINIHLASIGSGPALLFLHGFPEIWYTWRHQLLFFASRGFRAIAPDLR 60

Query: 61  GYGDSDVPPSPSSYTAHHIVGDLIGLLDHLEIDQVFLVGHDLGATIAWYFSIFRPDRVKA 120
           GYGDSDVPPSPSSYTAHHIVGDLIGLLDHLEIDQVFLVGHDLGATIAWYFSIFRPDRVKA
Sbjct: 61  GYGDSDVPPSPSSYTAHHIVGDLIGLLDHLEIDQVFLVGHDLGATIAWYFSIFRPDRVKA 120

Query: 121 LVNLSVYHTPRIPEMPPLAIIRLFLGDDFYICKFQEPGVAEADLGSVDTATMMKKFLTLR 180
           LVNLSVYHTPRIPEMPPLAIIRLFLGDDFYICKFQEPGVAEADLGSVDTATMMKKFLTLR
Sbjct: 121 LVNLSVYHTPRIPEMPPLAIIRLFLGDDFYICKFQEPGVAEADLGSVDTATMMKKFLTLR 180

Query: 181 DPSAPIAPNGFSTLLATPETLPSWLTEEDVDYYASKFAKTGFTGGLNYYRALDLTWELTG 240
           DPSAPIAPNGFSTLLATPETLPSWLTE+DVDYYASKFAKTGFTGGLNYYRALDLTWELTG
Sbjct: 181 DPSAPIAPNGFSTLLATPETLPSWLTEDDVDYYASKFAKTGFTGGLNYYRALDLTWELTG 240

Query: 241 PWTGAKIKVPTKFIVGDQDLVYHFPGAKEYIHGDSFKEDVPLLEEVVVIEGAAHFINQEK 300
           PWTGAKIKVPTKFIVGDQDLVYHFPGAKEYIHGDSFKEDVP LEEVVVIEGAAHFINQEK
Sbjct: 241 PWTGAKIKVPTKFIVGDQDLVYHFPGAKEYIHGDSFKEDVPHLEEVVVIEGAAHFINQEK 300

Query: 301 ADEINSLIYDFITKF 316
           ADEINSLIYDFITKF
Sbjct: 301 ADEINSLIYDFITKF 315

[Close]

BLAST of CSPI04G00950 vs. TrEMBL
Match: A0A0A0KTC4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G001900 PE=4 SV=1)

HSP 1 Score: 495.7 bits (1275), Expect = 4.1e-137
Identity = 234/319 (73.35%), Postives = 269/319 (84.33%), Query Frame = 1


Query: 1   MEKIQHSTIPTNGINIHLASIGSGPAVLFLHGFPEIWYTWRHQLLFFASKGFRAIAPDLR 60
           MEKI+H+TIPTNGIN+H+ASIGSGPAVLFLHGFP++WY+WRHQLLF ASKGFRA+APDLR
Sbjct: 1   MEKIEHTTIPTNGINMHVASIGSGPAVLFLHGFPQLWYSWRHQLLFLASKGFRALAPDLR 60

Query: 61  GYGDSDVPPSPSSYTAHHIVGDLIGLLDHLEIDQVFLVGHDLGATIAWYFSIFRPDRVKA 120
           G+GD+D PPSPSSYT HHI+GDLIGLLDH  +D+VFLVGHD GA IAWYF +FRPDRVKA
Sbjct: 61  GFGDTDAPPSPSSYTFHHIIGDLIGLLDHFGLDKVFLVGHDWGAVIAWYFCLFRPDRVKA 120

Query: 121 LVNLSVYHTPRIPEMPPLAIIRLFLGDDFYICKFQEPGVAEADLGSVDTATMMKKFLTLR 180
           LVNLSV++  R P +  +   R   G++FYIC+FQE GVAEAD GSVDTATMMKKF+ +R
Sbjct: 121 LVNLSVHYLKRHPSINFVDGFRASAGENFYICQFQEAGVAEADFGSVDTATMMKKFMGMR 180

Query: 181 DPSAPIAPN----GFSTLLATPETLPSWLTEEDVDYYASKFAKTGFTGGLNYYRALDLTW 240
           DP AP   N    GFS+ L TP  LP WLTEEDVD++ASKF+KTGFTGG NYYRAL+L+W
Sbjct: 181 DPVAPPIYNTKEKGFSS-LETPNPLPCWLTEEDVDFFASKFSKTGFTGGFNYYRALNLSW 240

Query: 241 ELTGPWTGAKIKVPTKFIVGDQDLVYHFPGAKEYIHGDSFKEDVPLLEEVVVIEGAAHFI 300
           ELT  W G+KI+VP KFIVGD DLVYHFPGAKEYI+G  FK+DVP LEEVVVI+ AAHFI
Sbjct: 241 ELTAAWNGSKIEVPVKFIVGDLDLVYHFPGAKEYINGGEFKKDVPFLEEVVVIKDAAHFI 300

Query: 301 NQEKADEINSLIYDFITKF 316
           NQEK  +INSLIY FI KF
Sbjct: 301 NQEKPHQINSLIYHFINKF 318

[Close]

BLAST of CSPI04G00950 vs. TrEMBL
Match: D8L7V8_PRUPE (Epoxide hydrolase 2 OS=Prunus persica GN=eh2 PE=2 SV=1)

HSP 1 Score: 466.1 bits (1198), Expect = 3.4e-128
Identity = 212/316 (67.09%), Postives = 250/316 (79.11%), Query Frame = 1


Query: 1   MEKIQHSTIPTNGINIHLASIGSGPAVLFLHGFPEIWYTWRHQLLFFASKGFRAIAPDLR 60
           MEKI+H+T+ TNGIN+H+ASIG+GP VLFLHGFPE+WY+WRHQLL  +S G+R IAPDLR
Sbjct: 1   MEKIEHTTVSTNGINMHIASIGTGPVVLFLHGFPELWYSWRHQLLSLSSLGYRCIAPDLR 60

Query: 61  GYGDSDVPPSPSSYTAHHIVGDLIGLLDHLEIDQVFLVGHDLGATIAWYFSIFRPDRVKA 120
           G+GD+D PPSP+SY+A HIVGDLIGLLDHL IDQVFLVGHD GA IAW+F +FRPDRVKA
Sbjct: 61  GFGDTDAPPSPASYSALHIVGDLIGLLDHLGIDQVFLVGHDWGAVIAWWFCLFRPDRVKA 120

Query: 121 LVNLSVYHTPRIPEMPPLAIIRLFLGDDFYICKFQEPGVAEADLGSVDTATMMKKFLTLR 180
           LVN+SV  +PR P+  P+   R   GDD+YIC+FQEPG  E +    DT ++MKKFLT R
Sbjct: 121 LVNMSVAFSPRNPKRKPVDGFRALFGDDYYICRFQEPGEIEKEFAGYDTTSIMKKFLTGR 180

Query: 181 DPSAPIAPNGFS-TLLATPETLPSWLTEEDVDYYASKFAKTGFTGGLNYYRALDLTWELT 240
            P  P  P         TPETLP WL+EED++Y+ASKF+KTGF GGLNYYRAL+LTWELT
Sbjct: 181 SPKPPCLPKELGLRAWKTPETLPPWLSEEDLNYFASKFSKTGFVGGLNYYRALNLTWELT 240

Query: 241 GPWTGAKIKVPTKFIVGDQDLVYHFPGAKEYIHGDSFKEDVPLLEEVVVIEGAAHFINQE 300
           GPWTG ++KVP KFIVGD D+ YH PG K YIH   FK DVP L+EVVVIE  AHFINQE
Sbjct: 241 GPWTGLQVKVPVKFIVGDLDITYHIPGVKNYIHNGGFKRDVPFLQEVVVIEDGAHFINQE 300

Query: 301 KADEINSLIYDFITKF 316
           + DEI+  +YDFI KF
Sbjct: 301 RPDEISRHVYDFIQKF 316

[Close]

BLAST of CSPI04G00950 vs. TrEMBL
Match: V4TVV1_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10021228mg PE=4 SV=1)

HSP 1 Score: 454.5 bits (1168), Expect = 1.0e-124
Identity = 211/317 (66.56%), Postives = 250/317 (78.86%), Query Frame = 1


Query: 1   MEKIQHSTIPTNGINIHLASIGSGPAVLFLHGFPEIWYTWRHQLLFFASKGFRAIAPDLR 60
           MEKI+H+T+ TNGIN+H+ASIG+GPAVLFLHGFPE+WY+WR QLL+ +S+G+RAIAPDLR
Sbjct: 1   MEKIKHTTVATNGINMHVASIGTGPAVLFLHGFPELWYSWRKQLLYLSSRGYRAIAPDLR 60

Query: 61  GYGDSDVPPSPSSYTAHHIVGDLIGLLDHLEIDQVFLVGHDLGATIAWYFSIFRPDRVKA 120
           GYGD+D PPS +SYTA H+VGDL+GLLD   I+QVFLVGHD GA IAWYF + RPDRVKA
Sbjct: 61  GYGDTDAPPSITSYTALHVVGDLVGLLDEFGIEQVFLVGHDWGALIAWYFCLLRPDRVKA 120

Query: 121 LVNLSVYHTPRIPEMPPLAIIRLFLGDDFYICKFQEPGVAEADLGSVDTATMMKKFLTLR 180
           LVNLSV    R P   P+   R   GDDFYIC+FQEPGVAE D   +DTA ++KKFL  R
Sbjct: 121 LVNLSVVFRSRNPATKPVDQYRALFGDDFYICRFQEPGVAEEDFAQIDTARLIKKFLGGR 180

Query: 181 DPSAPIAPN--GFSTLLATPETLPSWLTEEDVDYYASKFAKTGFTGGLNYYRALDLTWEL 240
            P  P  P   GF   L    TLPSWL+EEDV+YYASKF++ GFTGGLNYYR LDL WEL
Sbjct: 181 SPKPPCVPKEIGFRG-LPDLRTLPSWLSEEDVNYYASKFSQKGFTGGLNYYRCLDLNWEL 240

Query: 241 TGPWTGAKIKVPTKFIVGDQDLVYHFPGAKEYIHGDSFKEDVPLLEEVVVIEGAAHFINQ 300
             PWTGA+IK+P KF+VGD D+ YH PG +EYI    FK+DVP L+EV+V+EG AHFINQ
Sbjct: 241 LAPWTGAQIKIPVKFMVGDLDITYHIPGIREYIQNGGFKKDVPGLQEVIVMEGVAHFINQ 300

Query: 301 EKADEINSLIYDFITKF 316
           EKADE++S IYDFI +F
Sbjct: 301 EKADEVSSHIYDFIKQF 316

[Close]

BLAST of CSPI04G00950 vs. TrEMBL
Match: A0A067HGN0_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g021184mg PE=4 SV=1)

HSP 1 Score: 454.5 bits (1168), Expect = 1.0e-124
Identity = 211/317 (66.56%), Postives = 250/317 (78.86%), Query Frame = 1


Query: 1   MEKIQHSTIPTNGINIHLASIGSGPAVLFLHGFPEIWYTWRHQLLFFASKGFRAIAPDLR 60
           MEKI+H+T+ TNGIN+H+ASIG+GPAVLFLHGFPE+WY+WR QLL+ +S+G+RAIAPDLR
Sbjct: 1   MEKIKHTTVATNGINMHVASIGTGPAVLFLHGFPELWYSWRKQLLYLSSRGYRAIAPDLR 60

Query: 61  GYGDSDVPPSPSSYTAHHIVGDLIGLLDHLEIDQVFLVGHDLGATIAWYFSIFRPDRVKA 120
           GYGD+D PPS +SYTA H+VGDL+GLLD   I+QVFLVGHD GA IAWYF + RPDRVKA
Sbjct: 61  GYGDTDAPPSITSYTALHVVGDLVGLLDEFGIEQVFLVGHDWGALIAWYFCLLRPDRVKA 120

Query: 121 LVNLSVYHTPRIPEMPPLAIIRLFLGDDFYICKFQEPGVAEADLGSVDTATMMKKFLTLR 180
           LVNLSV    R P   P+   R   GDDFYIC+FQEPGVAE D   +DTA ++KKFL  R
Sbjct: 121 LVNLSVVFRSRNPATKPVDQYRALFGDDFYICRFQEPGVAEEDFAQIDTARLIKKFLGGR 180

Query: 181 DPSAPIAPN--GFSTLLATPETLPSWLTEEDVDYYASKFAKTGFTGGLNYYRALDLTWEL 240
            P  P  P   GF   L    TLPSWL+EEDV+YYASKF++ GFTGGLNYYR LDL WEL
Sbjct: 181 SPKPPCVPKEIGFRG-LPDLRTLPSWLSEEDVNYYASKFSQKGFTGGLNYYRCLDLNWEL 240

Query: 241 TGPWTGAKIKVPTKFIVGDQDLVYHFPGAKEYIHGDSFKEDVPLLEEVVVIEGAAHFINQ 300
             PWTGA+IK+P KF+VGD D+ YH PG +EYI    FK+DVP L+EV+V+EG AHFINQ
Sbjct: 241 LAPWTGAQIKIPVKFMVGDLDITYHIPGIREYIQNGGFKKDVPGLQEVIVMEGVAHFINQ 300

Query: 301 EKADEINSLIYDFITKF 316
           EKADE++S IYDFI +F
Sbjct: 301 EKADEVSSHIYDFIKQF 316

[Close]

BLAST of CSPI04G00950 vs. TAIR10
Match: AT4G02340.1 (AT4G02340.1 alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 426.4 bits (1095), Expect = 1.5e-119
Identity = 194/317 (61.20%), Postives = 237/317 (74.76%), Query Frame = 1


Query: 1   MEKIQHSTIPTNGINIHLASIGSGPAVLFLHGFPEIWYTWRHQLLFFASKGFRAIAPDLR 60
           MEKI+H+TI TNGIN+H+ASIGSGP +LF+HGFP++WY+WRHQL+ FA+ G+RAIAPDLR
Sbjct: 1   MEKIEHTTISTNGINMHVASIGSGPVILFVHGFPDLWYSWRHQLVSFAALGYRAIAPDLR 60

Query: 61  GYGDSDVPPSPSSYTAHHIVGDLIGLLDHLEIDQVFLVGHDLGATIAWYFSIFRPDRVKA 120
           GYGDSD PPS  SYT  HIVGDL+GLLD L +D+VFLVGHD GA +AW+  + RPDRV A
Sbjct: 61  GYGDSDAPPSRESYTILHIVGDLVGLLDSLGVDRVFLVGHDWGAIVAWWLCMIRPDRVNA 120

Query: 121 LVNLSVYHTPRIPEMPPLAIIRLFLGDDFYICKFQEPGVAEADLGSVDTATMMKKFLTLR 180
           LVN SV   PR P + P+   R   GDD+YIC+FQEPG  E D   VDT  ++ +F T R
Sbjct: 121 LVNTSVVFNPRNPSVKPVDAFRALFGDDYYICRFQEPGEIEEDFAQVDTKKLITRFFTSR 180

Query: 181 DPSAPIAPN--GFSTLLATPETLPSWLTEEDVDYYASKFAKTGFTGGLNYYRALDLTWEL 240
           +P  P  P   GF   L  P +LP+WLTE+DV +Y  KF++ GFTGGLNYYRAL+L+WEL
Sbjct: 181 NPRPPCIPKSVGFRG-LPDPPSLPAWLTEQDVRFYGDKFSQKGFTGGLNYYRALNLSWEL 240

Query: 241 TGPWTGAKIKVPTKFIVGDQDLVYHFPGAKEYIHGDSFKEDVPLLEEVVVIEGAAHFINQ 300
           T PWTG +IKVP KFIVGD D+ Y+ PG KEYIH    K+ VP L+EVVV+EG  HF++Q
Sbjct: 241 TAPWTGLQIKVPVKFIVGDLDITYNIPGTKEYIHEGGLKKHVPFLQEVVVMEGVGHFLHQ 300

Query: 301 EKADEINSLIYDFITKF 316
           EK DE+   IY F  KF
Sbjct: 301 EKPDEVTDHIYGFFKKF 316

[Close]

BLAST of CSPI04G00950 vs. TAIR10
Match: AT2G26740.1 (AT2G26740.1 soluble epoxide hydrolase)

HSP 1 Score: 374.0 bits (959), Expect = 9.0e-104
Identity = 185/321 (57.63%), Postives = 229/321 (71.34%), Query Frame = 1


Query: 4   IQHSTIPTNGINIHLASIG--SGPAVLFLHGFPEIWYTWRHQLLFFASKGFRAIAPDLRG 63
           ++H  +  NGI+IH+A  G   GP VL LHGFPE+WY+WRHQ+   A++G+RA+APDLRG
Sbjct: 1   MEHRKVRGNGIDIHVAIQGPSDGPIVLLLHGFPELWYSWRHQIPGLAARGYRAVAPDLRG 60

Query: 64  YGDSDVPPSPSSYTAHHIVGDLIGLLDHL---EIDQVFLVGHDLGATIAWYFSIFRPDRV 123
           YGDSD P   SSYT  +IVGDLI ++  L   E ++VF+VGHD GA IAWY  +FRPDRV
Sbjct: 61  YGDSDAPAEISSYTCFNIVGDLIAVISALTASEDEKVFVVGHDWGALIAWYLCLFRPDRV 120

Query: 124 KALVNLSVYHT--PRIPEMPPLAIIRLFLGDDFYICKFQEPGVAEADLGSVDTATMMKKF 183
           KALVNLSV  +  P  P + P+  +R F GDD+YIC+FQE G  EA++  V T  +MK+ 
Sbjct: 121 KALVNLSVPFSFRPTDPSVKPVDRMRAFYGDDYYICRFQEFGDVEAEIAEVGTERVMKRL 180

Query: 184 LTLRDPSAPIAPNGFSTLLATPET--LPSWLTEEDVDYYASKFAKTGFTGGLNYYRALDL 243
           LT R P   I P   S   +  ET  LPSWLTEEDV Y+ SKF + GF+G +NYYR  + 
Sbjct: 181 LTYRTPGPVIIPKDKSFWGSKGETIPLPSWLTEEDVAYFVSKFEEKGFSGPVNYYRNFNR 240

Query: 244 TWELTGPWTGAKIKVPTKFIVGDQDLVYHFPGAKEYIHGDSFKEDVPLLEEVVVIEGAAH 303
             EL GPW G+KI+VPTKF++G+ DLVY+ PG KEYIHG  FKEDVPLLEE VV+EG AH
Sbjct: 241 NNELLGPWVGSKIQVPTKFVIGELDLVYYMPGVKEYIHGPQFKEDVPLLEEPVVMEGVAH 300

Query: 304 FINQEKADEINSLIYDFITKF 316
           FINQEK  EI  +I DFI+KF
Sbjct: 301 FINQEKPQEILQIILDFISKF 321

[Close]

BLAST of CSPI04G00950 vs. TAIR10
Match: AT3G05600.1 (AT3G05600.1 alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 357.8 bits (917), Expect = 6.7e-99
Identity = 171/324 (52.78%), Postives = 216/324 (66.67%), Query Frame = 1


Query: 1   MEKIQHSTIPTNGINIHLASIG--SGPAVLFLHGFPEIWYTWRHQLLFFASKGFRAIAPD 60
           ME I H  +  NGI +H+A  G   GP VL LHGFP++WYTWRHQ+   +S G+RA+APD
Sbjct: 1   MEGIDHRMVSVNGITMHIAEKGPKEGPVVLLLHGFPDLWYTWRHQISGLSSLGYRAVAPD 60

Query: 61  LRGYGDSDVPPSPSSYTAHHIVGDLIGLLDHLEIDQ--VFLVGHDLGATIAWYFSIFRPD 120
           LRGYGDSD P S S YT  ++VGDL+ LLD +  +Q  VFLVGHD GA I W+  +FRP+
Sbjct: 61  LRGYGDSDSPESFSEYTCLNVVGDLVALLDSVAGNQEKVFLVGHDWGAIIGWFLCLFRPE 120

Query: 121 RVKALVNLSVYHTPRIPEMPPLAIIRLFLGDDFYICKFQEPGVAEADLGSVDTATMMKKF 180
           ++   V LSV +  R P++ P+   +   GDD+YIC+FQEPG  E ++ S D    ++  
Sbjct: 121 KINGFVCLSVPYRSRNPKVKPVQGFKAVFGDDYYICRFQEPGKIEGEIASADPRIFLRNL 180

Query: 181 LTLRDPSAPIAP--NGFSTLLATPET----LPSWLTEEDVDYYASKFAKTGFTGGLNYYR 240
            T R    PI P  N F      P +    LP W +++D+D+Y SKF K GFTGGLNYYR
Sbjct: 181 FTGRTLGPPILPKDNPFGEK-PNPNSENIELPEWFSKKDLDFYVSKFEKAGFTGGLNYYR 240

Query: 241 ALDLTWELTGPWTGAKIKVPTKFIVGDQDLVYHFPGAKEYIHGDSFKEDVPLLEEVVVIE 300
           A+DL WELT PWTGAKI+VP KF+ GD D+VY  PG KEYIHG  F  DVP L+E+VVIE
Sbjct: 241 AMDLNWELTAPWTGAKIQVPVKFMTGDFDMVYTTPGMKEYIHGGGFAADVPTLQEIVVIE 300

Query: 301 GAAHFINQEKADEINSLIYDFITK 315
            A HF+NQEK  E+ + I DF TK
Sbjct: 301 DAGHFVNQEKPQEVTAHINDFFTK 323

[Close]

BLAST of CSPI04G00950 vs. TAIR10
Match: AT2G26750.1 (AT2G26750.1 alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 357.5 bits (916), Expect = 8.7e-99
Identity = 175/320 (54.69%), Postives = 224/320 (70.00%), Query Frame = 1


Query: 4   IQHSTIPTNGINIHLASIG--SGPAVLFLHGFPEIWYTWRHQLLFFASKGFRAIAPDLRG 63
           ++H  +  NGI+IH+A  G   G  VL LHGFPE+WY+WRHQ+   A++G+RA+APDLRG
Sbjct: 1   MEHRNVRGNGIDIHVAIQGPSDGTIVLLLHGFPELWYSWRHQISGLAARGYRAVAPDLRG 60

Query: 64  YGDSDVPPSPSSYTAHHIVGDLIGLLDHL--EIDQVFLVGHDLGATIAWYFSIFRPDRVK 123
           YGDSD P   SS+T  +IVGDL+ ++  L  E  +VF+VGHD GA IAWY  +FRPD+VK
Sbjct: 61  YGDSDAPAEISSFTCFNIVGDLVAVISTLIKEDKKVFVVGHDWGALIAWYLCLFRPDKVK 120

Query: 124 ALVNLSVYHT--PRIPEMPPLAIIRLFLGDDFYICKFQEPGVAEADLGSVDTATMMKKFL 183
           ALVNLSV  +  P  P + P+  +R   G+D+Y+C+FQE G  EA++  V T  +MK+ L
Sbjct: 121 ALVNLSVPLSFWPTDPSVKPVDRMRAVYGNDYYVCRFQEVGDIEAEIAEVGTERVMKRLL 180

Query: 184 TLRDPSAPIAPNGFSTLLATPET--LPSWLTEEDVDYYASKFAKTGFTGGLNYYRALDLT 243
           T R P   I P   S   +  ET  LPSWLTEEDV Y+ SKF + GF G +NYYR  +  
Sbjct: 181 TYRTPGPLIIPKDKSFWGSKGETIPLPSWLTEEDVAYFVSKFKEKGFCGPVNYYRNFNRN 240

Query: 244 WELTGPWTGAKIKVPTKFIVGDQDLVYHFPGAKEYIHGDSFKEDVPLLEEVVVIEGAAHF 303
            EL GPW G+KI+VPTKF++G+ DLVY+ PG KEYIHG  FKEDVPL+EE VV+EG AHF
Sbjct: 241 NELLGPWVGSKIQVPTKFVIGELDLVYYMPGVKEYIHGPQFKEDVPLIEEPVVMEGVAHF 300

Query: 304 INQEKADEINSLIYDFITKF 316
           +NQEK  EI  +I DFI+ F
Sbjct: 301 LNQEKPQEILQIILDFISTF 320

[Close]

BLAST of CSPI04G00950 vs. TAIR10
Match: AT4G15960.1 (AT4G15960.1 alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 349.0 bits (894), Expect = 3.1e-96
Identity = 161/324 (49.69%), Postives = 218/324 (67.28%), Query Frame = 1


Query: 1   MEKIQHSTIPTNGINIHLAS---IGSG--PAVLFLHGFPEIWYTWRHQLLFFASKGFRAI 60
           ++ ++H T+  NGIN+H+A     GSG  P +LFLHGFPE+WYTWRHQ++  +S G+R I
Sbjct: 51  LDGVEHKTLKVNGINMHVAEKPGSGSGEDPIILFLHGFPELWYTWRHQMVALSSLGYRTI 110

Query: 61  APDLRGYGDSDVPPSPSSYTAHHIVGDLIGLLDHLEIDQ--VFLVGHDLGATIAWYFSIF 120
           APDLRGYGD++ P     YT  ++ GD++ L+D +      V +VGHD GA IAW    +
Sbjct: 111 APDLRGYGDTEAPEKVEDYTYLNVDGDVVALIDAVTGGDKAVSVVGHDWGAMIAWQLCQY 170

Query: 121 RPDRVKALVNLSVYHTPRIPEMPPLAIIRLFLGDDFYICKFQEPGVAEADLGSVDTATMM 180
           RP++VKALVN+SV  +PR P   P+  +R   GDD+Y+C+FQ+ G  E +   + T  ++
Sbjct: 171 RPEKVKALVNMSVLFSPRNPVRVPVPTLRHVFGDDYYVCRFQKAGEIETEFKKLGTENVL 230

Query: 181 KKFLTLRDPSAPIAPNG--FSTLLATPETLPSWLTEEDVDYYASKFAKTGFTGGLNYYRA 240
           K+FLT + P     P    F         LP WLT+ED+DYY +K+   GFTG +NYYR 
Sbjct: 231 KEFLTYKTPGPLNLPKDKYFKRSENAASALPLWLTQEDLDYYVTKYENKGFTGPINYYRN 290

Query: 241 LDLTWELTGPWTGAKIKVPTKFIVGDQDLVYHFPGAKEYIHGDSFKEDVPLLEEVVVIEG 300
           +D  WELT PWTGAKI+VP KFI+GDQDL Y+FPGAKEYI+G  FK DVPLL+E VV++G
Sbjct: 291 IDRNWELTAPWTGAKIRVPVKFIIGDQDLTYNFPGAKEYINGGGFKRDVPLLDETVVLKG 350

Query: 301 AAHFINQEKADEINSLIYDFITKF 316
             HF+++E  D IN  I++F  KF
Sbjct: 351 LGHFLHEENPDVINQHIHNFFHKF 374

[Close]

BLAST of CSPI04G00950 vs. NCBI nr
Match: gi|449469070|ref|XP_004152244.1| (PREDICTED: bifunctional epoxide hydrolase 2-like [Cucumis sativus])

HSP 1 Score: 654.4 bits (1687), Expect = 9.8e-185
Identity = 311/315 (98.73%), Postives = 314/315 (99.68%), Query Frame = 1


Query: 1   MEKIQHSTIPTNGINIHLASIGSGPAVLFLHGFPEIWYTWRHQLLFFASKGFRAIAPDLR 60
           MEKIQHSTIPTNGINIHLASIGSGPA+LFLHGFPEIWYTWRHQLLFFAS+GFRAIAPDLR
Sbjct: 1   MEKIQHSTIPTNGINIHLASIGSGPALLFLHGFPEIWYTWRHQLLFFASRGFRAIAPDLR 60

Query: 61  GYGDSDVPPSPSSYTAHHIVGDLIGLLDHLEIDQVFLVGHDLGATIAWYFSIFRPDRVKA 120
           GYGDSDVPPSPSSYTAHHIVGDLIGLLDHLEIDQVFLVGHDLGATIAWYFSIFRPDRVKA
Sbjct: 61  GYGDSDVPPSPSSYTAHHIVGDLIGLLDHLEIDQVFLVGHDLGATIAWYFSIFRPDRVKA 120

Query: 121 LVNLSVYHTPRIPEMPPLAIIRLFLGDDFYICKFQEPGVAEADLGSVDTATMMKKFLTLR 180
           LVNLSVYHTPRIPEMPPLAIIRLFLGDDFYICKFQEPGVAEADLGSVDTATMMKKFLTLR
Sbjct: 121 LVNLSVYHTPRIPEMPPLAIIRLFLGDDFYICKFQEPGVAEADLGSVDTATMMKKFLTLR 180

Query: 181 DPSAPIAPNGFSTLLATPETLPSWLTEEDVDYYASKFAKTGFTGGLNYYRALDLTWELTG 240
           DPSAPIAPNGFSTLLATPETLPSWLTE+DVDYYASKFAKTGFTGGLNYYRALDLTWELTG
Sbjct: 181 DPSAPIAPNGFSTLLATPETLPSWLTEDDVDYYASKFAKTGFTGGLNYYRALDLTWELTG 240

Query: 241 PWTGAKIKVPTKFIVGDQDLVYHFPGAKEYIHGDSFKEDVPLLEEVVVIEGAAHFINQEK 300
           PWTGAKIKVPTKFIVGDQDLVYHFPGAKEYIHGDSFKEDVP LEEVVVIEGAAHFINQEK
Sbjct: 241 PWTGAKIKVPTKFIVGDQDLVYHFPGAKEYIHGDSFKEDVPHLEEVVVIEGAAHFINQEK 300

Query: 301 ADEINSLIYDFITKF 316
           ADEINSLIYDFITKF
Sbjct: 301 ADEINSLIYDFITKF 315

[Close]

BLAST of CSPI04G00950 vs. NCBI nr
Match: gi|659108671|ref|XP_008454327.1| (PREDICTED: bifunctional epoxide hydrolase 2-like [Cucumis melo])

HSP 1 Score: 565.1 bits (1455), Expect = 7.8e-158
Identity = 263/315 (83.49%), Postives = 285/315 (90.48%), Query Frame = 1


Query: 1   MEKIQHSTIPTNGINIHLASIGSGPAVLFLHGFPEIWYTWRHQLLFFASKGFRAIAPDLR 60
           M+KIQHSTI TNGINIH ASIGSGP VLFLHGFPE+WY+WRHQLLF ASKGFRAIAPDLR
Sbjct: 1   MDKIQHSTISTNGINIHFASIGSGPVVLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLR 60

Query: 61  GYGDSDVPPSPSSYTAHHIVGDLIGLLDHLEIDQVFLVGHDLGATIAWYFSIFRPDRVKA 120
           G+GDSD PPSPSSYT HHIVGDLIGLLDHL IDQVFLVGHD GA +AWYF +FRPDRVKA
Sbjct: 61  GFGDSDAPPSPSSYTPHHIVGDLIGLLDHLGIDQVFLVGHDWGAMMAWYFCLFRPDRVKA 120

Query: 121 LVNLSVYHTPRIPEMPPLAIIRLFLGDDFYICKFQEPGVAEADLGSVDTATMMKKFLTLR 180
           LVNLSV++TPR P   PLA+ R +LGDDFYICKFQEPGVAEAD GSVDTATMMKKFLT+R
Sbjct: 121 LVNLSVHYTPRNPAGSPLAVTRRYLGDDFYICKFQEPGVAEADFGSVDTATMMKKFLTMR 180

Query: 181 DPSAPIAPNGFSTLLATPETLPSWLTEEDVDYYASKFAKTGFTGGLNYYRALDLTWELTG 240
           DP   I PNGF TLL TPE LPSWLTEED++Y+ASKF+KTGFTGG NYYRALD+TWELTG
Sbjct: 181 DPRPAIIPNGFKTLLETPEILPSWLTEEDIEYFASKFSKTGFTGGFNYYRALDITWELTG 240

Query: 241 PWTGAKIKVPTKFIVGDQDLVYHFPGAKEYIHGDSFKEDVPLLEEVVVIEGAAHFINQEK 300
           PW+ A+IKVPTKFIVGD DLVY+FPGAKEYIHG  FK+DVPLLE+VVVIEGAAHFINQEK
Sbjct: 241 PWSRAQIKVPTKFIVGDLDLVYNFPGAKEYIHGGGFKKDVPLLEDVVVIEGAAHFINQEK 300

Query: 301 ADEINSLIYDFITKF 316
            DEI+SLIYDFITKF
Sbjct: 301 PDEISSLIYDFITKF 315

[Close]

BLAST of CSPI04G00950 vs. NCBI nr
Match: gi|449469304|ref|XP_004152361.1| (PREDICTED: bifunctional epoxide hydrolase 2-like [Cucumis sativus])

HSP 1 Score: 495.7 bits (1275), Expect = 5.8e-137
Identity = 234/319 (73.35%), Postives = 269/319 (84.33%), Query Frame = 1


Query: 1   MEKIQHSTIPTNGINIHLASIGSGPAVLFLHGFPEIWYTWRHQLLFFASKGFRAIAPDLR 60
           MEKI+H+TIPTNGIN+H+ASIGSGPAVLFLHGFP++WY+WRHQLLF ASKGFRA+APDLR
Sbjct: 1   MEKIEHTTIPTNGINMHVASIGSGPAVLFLHGFPQLWYSWRHQLLFLASKGFRALAPDLR 60

Query: 61  GYGDSDVPPSPSSYTAHHIVGDLIGLLDHLEIDQVFLVGHDLGATIAWYFSIFRPDRVKA 120
           G+GD+D PPSPSSYT HHI+GDLIGLLDH  +D+VFLVGHD GA IAWYF +FRPDRVKA
Sbjct: 61  GFGDTDAPPSPSSYTFHHIIGDLIGLLDHFGLDKVFLVGHDWGAVIAWYFCLFRPDRVKA 120

Query: 121 LVNLSVYHTPRIPEMPPLAIIRLFLGDDFYICKFQEPGVAEADLGSVDTATMMKKFLTLR 180
           LVNLSV++  R P +  +   R   G++FYIC+FQE GVAEAD GSVDTATMMKKF+ +R
Sbjct: 121 LVNLSVHYLKRHPSINFVDGFRASAGENFYICQFQEAGVAEADFGSVDTATMMKKFMGMR 180

Query: 181 DPSAPIAPN----GFSTLLATPETLPSWLTEEDVDYYASKFAKTGFTGGLNYYRALDLTW 240
           DP AP   N    GFS+ L TP  LP WLTEEDVD++ASKF+KTGFTGG NYYRAL+L+W
Sbjct: 181 DPVAPPIYNTKEKGFSS-LETPNPLPCWLTEEDVDFFASKFSKTGFTGGFNYYRALNLSW 240

Query: 241 ELTGPWTGAKIKVPTKFIVGDQDLVYHFPGAKEYIHGDSFKEDVPLLEEVVVIEGAAHFI 300
           ELT  W G+KI+VP KFIVGD DLVYHFPGAKEYI+G  FK+DVP LEEVVVI+ AAHFI
Sbjct: 241 ELTAAWNGSKIEVPVKFIVGDLDLVYHFPGAKEYINGGEFKKDVPFLEEVVVIKDAAHFI 300

Query: 301 NQEKADEINSLIYDFITKF 316
           NQEK  +INSLIY FI KF
Sbjct: 301 NQEKPHQINSLIYHFINKF 318

[Close]

BLAST of CSPI04G00950 vs. NCBI nr
Match: gi|659108669|ref|XP_008454325.1| (PREDICTED: bifunctional epoxide hydrolase 2-like [Cucumis melo])

HSP 1 Score: 493.0 bits (1268), Expect = 3.8e-136
Identity = 229/318 (72.01%), Postives = 269/318 (84.59%), Query Frame = 1


Query: 1   MEKIQHSTIPTNGINIHLASIGSGPAVLFLHGFPEIWYTWRHQLLFFASKGFRAIAPDLR 60
           MEKI+H+TIPTNGIN+H+ASIGSGPAVLFLHGFP++WY+WRHQLLF ASKGFRA+APDLR
Sbjct: 1   MEKIEHTTIPTNGINMHVASIGSGPAVLFLHGFPQLWYSWRHQLLFLASKGFRALAPDLR 60

Query: 61  GYGDSDVPPSPSSYTAHHIVGDLIGLLDHLEIDQVFLVGHDLGATIAWYFSIFRPDRVKA 120
           G+GD+D PPSPSSYT  HI+GDLIGLLDHL +++VFLVGHD GA IAWYF +FRPDRVKA
Sbjct: 61  GFGDTDAPPSPSSYTFLHIIGDLIGLLDHLGLEKVFLVGHDWGAMIAWYFCLFRPDRVKA 120

Query: 121 LVNLSVYHTPRIPEMPPLAIIRLFLGDDFYICKFQEPGVAEADLGSVDTATMMKKFLTLR 180
           LVNLSVY+  R P +  +   R   GD+FYIC+FQE GVAEAD G VDTATMMKKF+ +R
Sbjct: 121 LVNLSVYYIKRHPSISFVDGFRAVAGDNFYICQFQEAGVAEADFGRVDTATMMKKFMGMR 180

Query: 181 DPSAPI---APNGFSTLLATPETLPSWLTEEDVDYYASKFAKTGFTGGLNYYRALDLTWE 240
           DP AP+      GFS+ + TP+ LP WLTEED+D++A+KF+KTGFTGG NYYRAL+L+WE
Sbjct: 181 DPEAPLIFTKEKGFSS-METPDPLPCWLTEEDIDFFATKFSKTGFTGGFNYYRALNLSWE 240

Query: 241 LTGPWTGAKIKVPTKFIVGDQDLVYHFPGAKEYIHGDSFKEDVPLLEEVVVIEGAAHFIN 300
           LT  W G+KI+VP KFIVGD DLVYHFPGAK+YIHG  FK+DVP LEEVVVI+ AAHFI+
Sbjct: 241 LTAAWNGSKIEVPVKFIVGDLDLVYHFPGAKQYIHGGEFKKDVPFLEEVVVIKDAAHFIH 300

Query: 301 QEKADEINSLIYDFITKF 316
           QEK  +INSLIY FI KF
Sbjct: 301 QEKPHQINSLIYHFINKF 317

[Close]

BLAST of CSPI04G00950 vs. NCBI nr
Match: gi|658039812|ref|XP_008355493.1| (PREDICTED: bifunctional epoxide hydrolase 2-like [Malus domestica])

HSP 1 Score: 469.9 bits (1208), Expect = 3.4e-129
Identity = 216/317 (68.14%), Postives = 254/317 (80.13%), Query Frame = 1


Query: 1   MEKIQHSTIPTNGINIHLASIGSGPAVLFLHGFPEIWYTWRHQLLFFASKGFRAIAPDLR 60
           ME I+H+T+PTNGIN+H+ASIG+GP VLFLHGFPE+WY+WRHQLL  +S G+R IAPDLR
Sbjct: 1   MENIEHTTVPTNGINMHVASIGTGPPVLFLHGFPELWYSWRHQLLSLSSLGYRCIAPDLR 60

Query: 61  GYGDSDVPPSPSSYTAHHIVGDLIGLLDHLEIDQVFLVGHDLGATIAWYFSIFRPDRVKA 120
           G+GD+D PPSP+SYTA HIVGDLIGLLDHL IDQVFLV HD GA +AW+F +FRPDRVKA
Sbjct: 61  GFGDTDAPPSPTSYTAMHIVGDLIGLLDHLGIDQVFLVAHDWGAVMAWWFCLFRPDRVKA 120

Query: 121 LVNLSVYHTPRIPEMPPLAIIRLFLGDDFYICKFQEPGVAEADLGSVDTATMMKKFLTLR 180
           LVN+SV  +PR P+  P+   R   GDD+YIC+FQEPG  E D  S+DTA +MK FLT R
Sbjct: 121 LVNMSVAFSPRNPKRKPIDTFRALFGDDYYICRFQEPGEIEQDFDSIDTAAVMKCFLTGR 180

Query: 181 DPSAPIAP--NGFSTLLATPETLPSWLTEEDVDYYASKFAKTGFTGGLNYYRALDLTWEL 240
            P  P  P   G  +  A P TLP+WLTEEDV+Y+ASKF+KTGFTGGLNYYRAL+LTWEL
Sbjct: 181 SPKPPCIPKDQGLKS-WAAPVTLPAWLTEEDVNYFASKFSKTGFTGGLNYYRALNLTWEL 240

Query: 241 TGPWTGAKIKVPTKFIVGDQDLVYHFPGAKEYIHGDSFKEDVPLLEEVVVIEGAAHFINQ 300
           TGPWTG +IKVP KFIVGD D+ YH PG + YIH   FK DVP L+EVVV+E AAHFINQ
Sbjct: 241 TGPWTGLQIKVPVKFIVGDLDITYHIPGIQNYIHKGGFKRDVPFLQEVVVMEDAAHFINQ 300

Query: 301 EKADEINSLIYDFITKF 316
           EK DE++  +YDFI KF
Sbjct: 301 EKPDEVSQHVYDFIKKF 316

The following BLAST results are available for this feature:

BLAST of CSPI04G00950 vs. Swiss-Prot


Match Name	E-value	Identity	Description
HYES_MOUSE	8.6e-48	33.66	Bifunctional epoxide hydrolase 2 OS=Mus musculus GN=Ephx2 PE=1 SV=2	[more]
HYES_PIG	1.1e-47	33.33	Bifunctional epoxide hydrolase 2 OS=Sus scrofa GN=EPHX2 PE=2 SV=1	[more]
HYES_RAT	4.3e-47	33.01	Bifunctional epoxide hydrolase 2 OS=Rattus norvegicus GN=Ephx2 PE=1 SV=1	[more]
HYES_HUMAN	6.2e-46	32.13	Bifunctional epoxide hydrolase 2 OS=Homo sapiens GN=EPHX2 PE=1 SV=2	[more]
EPHX3_HUMAN	1.8e-29	31.60	Epoxide hydrolase 3 OS=Homo sapiens GN=EPHX3 PE=2 SV=1	[more]

BLAST of CSPI04G00950 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KWK9_CUCSA	6.8e-185	98.73	Epoxide hydrolase 2 OS=Cucumis sativus GN=Csa_4G001890 PE=4 SV=1	[more]
A0A0A0KTC4_CUCSA	4.1e-137	73.35	Uncharacterized protein OS=Cucumis sativus GN=Csa_4G001900 PE=4 SV=1	[more]
D8L7V8_PRUPE	3.4e-128	67.09	Epoxide hydrolase 2 OS=Prunus persica GN=eh2 PE=2 SV=1	[more]
V4TVV1_9ROSI	1.0e-124	66.56	Uncharacterized protein OS=Citrus clementina GN=CICLE_v10021228mg PE=4 SV=1	[more]
A0A067HGN0_CITSI	1.0e-124	66.56	Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g021184mg PE=4 SV=1	[more]

BLAST of CSPI04G00950 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G02340.1	1.5e-119	61.20	alpha/beta-Hydrolases superfamily protein	[more]
AT2G26740.1	9.0e-104	57.63	soluble epoxide hydrolase	[more]
AT3G05600.1	6.7e-99	52.78	alpha/beta-Hydrolases superfamily protein	[more]
AT2G26750.1	8.7e-99	54.69	alpha/beta-Hydrolases superfamily protein	[more]
AT4G15960.1	3.1e-96	49.69	alpha/beta-Hydrolases superfamily protein	[more]

BLAST of CSPI04G00950 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449469070\|ref\|XP_004152244.1\|	9.8e-185	98.73	PREDICTED: bifunctional epoxide hydrolase 2-like [Cucumis sativus]	[more]
gi\|659108671\|ref\|XP_008454327.1\|	7.8e-158	83.49	PREDICTED: bifunctional epoxide hydrolase 2-like [Cucumis melo]	[more]
gi\|449469304\|ref\|XP_004152361.1\|	5.8e-137	73.35	PREDICTED: bifunctional epoxide hydrolase 2-like [Cucumis sativus]	[more]
gi\|659108669\|ref\|XP_008454325.1\|	3.8e-136	72.01	PREDICTED: bifunctional epoxide hydrolase 2-like [Cucumis melo]	[more]
gi\|658039812\|ref\|XP_008355493.1\|	3.4e-129	68.14	PREDICTED: bifunctional epoxide hydrolase 2-like [Malus domestica]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR000073	AB_hydrolase_1
IPR000639	Epox_hydrolase-like

Vocabulary: **Molecular Function**
Term	Definition
GO:0003824	catalytic activity

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008152	metabolic process
biological_process	GO:0018108	peptidyl-tyrosine phosphorylation
biological_process	GO:0008150	biological_process
cellular_component	GO:0005777	peroxisome
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0016787	hydrolase activity
molecular_function	GO:0003824	catalytic activity
molecular_function	GO:0004715	non-membrane spanning protein tyrosine kinase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI04G00950.1	CSPI04G00950.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000073	Alpha/beta hydrolase fold-1	PRINTS	PR00111	ABHYDROLASE	coord: 97..110 score: 1.6E-7coord: 111..124 score: 1.6E-7coord: 51..66 score: 1.
IPR000073	Alpha/beta hydrolase fold-1	PFAM	PF00561	Abhydrolase_1	coord: 25..140 score: 4.3
IPR000639	Epoxide hydrolase-like	PRINTS	PR00412	EPOXHYDRLASE	coord: 289..311 score: 2.4E-41coord: 51..66 score: 2.4E-41coord: 111..124 score: 2.4E-41coord: 97..110 score: 2.4E-41coord: 31..49 score: 2.4E-41coord: 249..265 score: 2.4
None	No IPR available	PANTHER	PTHR10992	ALPHA/BETA HYDROLASE FOLD-CONTAINING PROTEIN	coord: 1..165 score: 1.6E-165coord: 199..314 score: 1.6E
None	No IPR available	PANTHER	PTHR10992:SF811	ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEIN	coord: 1..165 score: 1.6E-165coord: 199..314 score: 1.6E

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CSPI04G00950	Cla017397	Watermelon (97103) v1	cpiwmB291
CSPI04G00950	MELO3C017945	Melon (DHL92) v3.5.1	cpimeB310
CSPI04G00950	MELO3C017945.2	Melon (DHL92) v3.6.1	cpimedB301
CSPI04G00950	CsaV3_4G000910	Cucumber (Chinese Long) v3	cpicucB213
CSPI04G00950	CmaCh14G009130	Cucurbita maxima (Rimu)	cmacpiB264
CSPI04G00950	CmoCh01G003170	Cucurbita moschata (Rifu)	cmocpiB468
CSPI04G00950	Cp4.1LG12g05740	Cucurbita pepo (Zucchini)	cpecpiB141

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CSPI04G00950	Watermelon (97103) v2	cpiwmbB278
CSPI04G00950	Watermelon (97103) v2	cpiwmbB346
CSPI04G00950	Wax gourd	cpiwgoB358
CSPI04G00950	Wild cucumber (PI 183967)	cpicpiB167
CSPI04G00950	Cucumber (Gy14) v1	cgycpiB103
CSPI04G00950	Cucumber (Gy14) v1	cgycpiB324
CSPI04G00950	Cucurbita maxima (Rimu)	cmacpiB369
CSPI04G00950	Cucurbita maxima (Rimu)	cmacpiB478
CSPI04G00950	Cucurbita maxima (Rimu)	cmacpiB913
CSPI04G00950	Cucurbita moschata (Rifu)	cmocpiB251
CSPI04G00950	Cucurbita moschata (Rifu)	cmocpiB359
CSPI04G00950	Cucurbita moschata (Rifu)	cmocpiB892
CSPI04G00950	Cucumber (Chinese Long) v2	cpicuB182
CSPI04G00950	Watermelon (Charleston Gray)	cpiwcgB280
CSPI04G00950	Cucurbita pepo (Zucchini)	cpecpiB316
CSPI04G00950	Cucurbita pepo (Zucchini)	cpecpiB568
CSPI04G00950	Cucurbita pepo (Zucchini)	cpecpiB618
CSPI04G00950	Bottle gourd (USVL1VR-Ls)	cpilsiB266
CSPI04G00950	Bottle gourd (USVL1VR-Ls)	cpilsiB274
CSPI04G00950	Cucumber (Gy14) v2	cgybcpiB168
CSPI04G00950	Silver-seed gourd	carcpiB0121
CSPI04G00950	Silver-seed gourd	carcpiB0800
CSPI04G00950	Silver-seed gourd	carcpiB0854
CSPI04G00950	Cucumber (Chinese Long) v3	cpicucB246