CSPI04G00340 (gene) Wild cucumber (PI 183967) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CSPI04G00340
Type	gene
Organism	Cucumis sativus (Wild cucumber (PI 183967))
Description	Trypsin inhibitor 1
Location	Chr4 : 194170 .. 195164 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CCCACTACAGACCCTCCTCGTTTTTCTTACATAATTCTTAATTATTTATTCTGAGTGATATCAAATAAATAAGGGGTTTCTTTATAATAAAAAATACAAATCAATGGTCCCTTTTTTTCTTTTTTCTTTTTTTTACAATTTCTCAATAAAAATAATGCACCCAAATGAGTTGTTCTCTCTATAAATATAGAGAGTTGAGATCATTGAGATGGTGTCAAGAAAGAGAGAGAAGCAAAGAAGGAAAAAATAATGGAGTGGAAAAAAGTTGCTTTAGTTGCAATGATGGGGATGTTGCTTATGGCTACTTTTACAGAGTCAGTCGGTTTGGGCGTGGATGAGGAGATCATTCAACTAGTTTCGGACGGGCTGAATGAGTACTCACAAAATATTGGAAAGGAAACTGTAGGCTGCCCTCGAATCCTTATGCCGTGTAAATCCGACCACGACTGCTTAAGTGGCTGCACTTGCAAACAAAACGGCTATTGTGGTTGACTCCTAGCCGTGATGTTTCGTTCGGATCTTCTACGGTGTCTTTGTTTGTGTGTGTTTTCTATTTTATGAAAATAAATGCATGGTTGTTGTGTGTCTTTCTCATCACTCTTTTCTCTCGTGTGTATGAGTTAGTGTGATCATATCAGTGGCATGCCTGATATCCCATCAAGCTGTTTTGTTGTAATATTTATTATGCTCTCTATCTCACTATGGTCTTTGAGGATATCTTCCCTATCTCCTTCCATCAACTTTAAGTTTCATTTTCTTTAACCATTCTTCCATGAATTTAAAGTTTTATTTTTTTTAACCATTAAATACTCAAAGATTAACTGTTAATCACTCAAAATTCATTTTTTTTAAACTATAGTTTTGAGTGTTTATCATATACTACATCATTTTATAACAAACACCTTCACGTGGATGTGGGCAAATCATAGTGAAGCTTGAGAGGTTTCTTTGAAGAGAGTAAAGTAGAGAGACAGACGGACCAAATTTTCTTCTTT

mRNA sequence

ATGGAGTGGAAAAAAGTTGCTTTAGTTGCAATGATGGGGATGTTGCTTATGGCTACTTTTACAGAGTCAGTCGGTTTGGGCGTGGATGAGGAGATCATTCAACTAGTTTCGGACGGGCTGAATGAGTACTCACAAAATATTGGAAAGGAAACTGTAGGCTGCCCTCGAATCCTTATGCCGTGTAAATCCGACCACGACTGCTTAAGTGGCTGCACTTGCAAACAAAACGGCTATTGTGGTTGA

Coding sequence (CDS)

ATGGAGTGGAAAAAAGTTGCTTTAGTTGCAATGATGGGGATGTTGCTTATGGCTACTTTTACAGAGTCAGTCGGTTTGGGCGTGGATGAGGAGATCATTCAACTAGTTTCGGACGGGCTGAATGAGTACTCACAAAATATTGGAAAGGAAACTGTAGGCTGCCCTCGAATCCTTATGCCGTGTAAATCCGACCACGACTGCTTAAGTGGCTGCACTTGCAAACAAAACGGCTATTGTGGTTGA

Homology

[Close]

BLAST of CSPI04G00340 vs. Swiss-Prot
Match: ITR1_TRIKI (Trypsin inhibitor 1 OS=Trichosanthes kirilowii PE=3 SV=1)

HSP 1 Score: 85.9 bits (211), Expect = 2.2e-16
Identity = 39/65 (60.00%), Postives = 46/65 (70.77%), Query Frame = 1


Query: 17 MATFTESVGLGVD-EEIIQLVSDGLNEYSQNIGKETVGCPRILMPCKSDHDCLSGCTCKQ 76
          MA F ES   G   +E+IQLVSDG+NEYS+ + +  V CPRILMPCK + DCL GC C  
Sbjct: 1  MAAFVESARAGAGADEVIQLVSDGVNEYSEKMMEGVVACPRILMPCKVNDDCLRGCKCLS 60

Query: 77 NGYCG 81
          NGYCG
Sbjct: 61 NGYCG 65

[Close]

BLAST of CSPI04G00340 vs. Swiss-Prot
Match: ITR5_LUFAE (Trypsin inhibitor 5 OS=Luffa aegyptiaca PE=1 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 8.2e-11
Identity = 33/66 (50.00%), Postives = 43/66 (65.15%), Query Frame = 1


Query: 17 MATFTESVGLGVDEEIIQLVSDGLNEYSQNI--GKETVGCPRILMPCKSDHDCLSGCTCK 76
          MA+  ES G+    E+I+L+SDG N+  + I  G+    CPRILMPCK+D DC+  C C 
Sbjct: 1  MASVAESSGV---VEVIELISDGGNDLPRKIMSGRHGGICPRILMPCKTDDDCMLDCRCL 60

Query: 77 QNGYCG 81
           NGYCG
Sbjct: 61 SNGYCG 63

[Close]

BLAST of CSPI04G00340 vs. Swiss-Prot
Match: ITR2_BRYDI (Trypsin inhibitor 2 OS=Bryonia dioica PE=1 SV=1)

HSP 1 Score: 57.4 bits (137), Expect = 8.5e-08
Identity = 21/28 (75.00%), Postives = 24/28 (85.71%), Query Frame = 1


Query: 53 GCPRILMPCKSDHDCLSGCTCKQNGYCG 81
          GCPRILM CK D DCL+GC C++NGYCG
Sbjct: 2  GCPRILMRCKRDSDCLAGCVCQKNGYCG 29

[Close]

BLAST of CSPI04G00340 vs. Swiss-Prot
Match: ITR2_ECBEL (Trypsin inhibitor 2 OS=Ecballium elaterium PE=1 SV=2)

HSP 1 Score: 54.3 bits (129), Expect = 7.2e-07
Identity = 20/28 (71.43%), Postives = 22/28 (78.57%), Query Frame = 1


Query: 53 GCPRILMPCKSDHDCLSGCTCKQNGYCG 81
          GCPRILM CK D DCL+GC C  NG+CG
Sbjct: 1  GCPRILMRCKQDSDCLAGCVCGPNGFCG 28

[Close]

BLAST of CSPI04G00340 vs. Swiss-Prot
Match: ITR4_LUFAE (Trypsin inhibitor 4 OS=Luffa aegyptiaca PE=1 SV=1)

HSP 1 Score: 52.8 bits (125), Expect = 2.1e-06
Identity = 19/27 (70.37%), Postives = 22/27 (81.48%), Query Frame = 1


Query: 54 CPRILMPCKSDHDCLSGCTCKQNGYCG 81
          CPRILMPC SD DCL+ C C +NG+CG
Sbjct: 2  CPRILMPCSSDSDCLAECICLENGFCG 28

[Close]

BLAST of CSPI04G00340 vs. TrEMBL
Match: A0A0A0KY34_CUCSA (Trypsin inhibitor 1 OS=Cucumis sativus GN=Csa_4G000810 PE=3 SV=1)

HSP 1 Score: 144.1 bits (362), Expect = 7.7e-32
Identity = 69/81 (85.19%), Postives = 73/81 (90.12%), Query Frame = 1


Query: 1  MEWKKVALVAMMGMLLMATFTESVGLGVDEEIIQLVSDGLNEYSQNIGKETV-GCPRILM 60
          MEWKK+ALVAMMGMLLMATFTESVGLGVDEEIIQLVSDG+NEYSQNIGK+T  GCPRILM
Sbjct: 1  MEWKKIALVAMMGMLLMATFTESVGLGVDEEIIQLVSDGVNEYSQNIGKDTAPGCPRILM 60

Query: 61 PCKSDHDCLSGCTCKQNGYCG 81
           CK+D DC  GCTCK  GYCG
Sbjct: 61 KCKTDSDCYPGCTCKPMGYCG 81

[Close]

BLAST of CSPI04G00340 vs. TrEMBL
Match: A0A0A0KT01_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G000805 PE=3 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 1.1e-09
Identity = 33/83 (39.76%), Postives = 51/83 (61.45%), Query Frame = 1


Query: 1  MEWKKVALVAMMGMLLMATFTESVGLGVDEEIIQLVSDGLNEYS---QNIGKETVGCPRI 60
          MEWKK+ ++A++GML+MA   E     ++ E ++    GLN+     + + +ET  CPRI
Sbjct: 1  MEWKKIGMLAVVGMLVMAALVEDCHAAIESESVK----GLNKNELPRKMMNEETRMCPRI 60

Query: 61 LMPCKSDHDCLSGCTCKQNGYCG 81
          L  C +D DC++ C C  NG+CG
Sbjct: 61 LEECTTDDDCMNDCICLSNGFCG 79

[Close]

BLAST of CSPI04G00340 vs. TrEMBL
Match: Q9S8D2_CUCME (CMETI-B=TRYPSIN inhibitor OS=Cucumis melo PE=3 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 1.0e-07
Identity = 24/29 (82.76%), Postives = 27/29 (93.10%), Query Frame = 1


Query: 52 VGCPRILMPCKSDHDCLSGCTCKQNGYCG 81
          VGCPRILM CK+D DCL+GCTCK+NGYCG
Sbjct: 1  VGCPRILMKCKTDRDCLTGCTCKRNGYCG 29

[Close]

BLAST of CSPI04G00340 vs. TrEMBL
Match: A0A0A7HF87_9ROSI (TIPTOP4 protein OS=Momordica subangulata GN=TIPTOP4 PE=3 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 5.0e-07
Identity = 34/80 (42.50%), Postives = 45/80 (56.25%), Query Frame = 1


Query: 1  MEWKKVALVAMMGMLLMATFTESVGLGVDEEIIQLVSDGLNEYSQNIGKETVGCPRILMP 60
          ME KK+ LV ++ M+L+AT T       D +   L+SDG  +   N G     CP+IL  
Sbjct: 1  MESKKILLVVLVAMMLVATST----CFNDGDTTDLISDGRAQMDINGG----ACPKILQR 60

Query: 61 CKSDHDCLSGCTCKQNGYCG 81
          C+ D DC S C C+ NGYCG
Sbjct: 61 CRRDSDCPSACICRGNGYCG 72

[Close]

BLAST of CSPI04G00340 vs. TrEMBL
Match: J3RCD6_MOMCO (Two inhibitor peptide topologies 1 OS=Momordica cochinchinensis GN=TIPTOP1 PE=2 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 1.1e-06
Identity = 34/80 (42.50%), Postives = 46/80 (57.50%), Query Frame = 1


Query: 1  MEWKKVALVAMMGMLLMATFTESVGLGVDEEIIQLVSDGLNEYSQNIGKETVGCPRILMP 60
          ME KK+  V ++ M+L+AT   S G   D + I+L+SDG  +   N G     CP+IL  
Sbjct: 1  MESKKILPVVLVAMMLVAT---STGFN-DGDTIELISDGRAQIDINGGV----CPKILQR 60

Query: 61 CKSDHDCLSGCTCKQNGYCG 81
          C+ D DC   C C+ NGYCG
Sbjct: 61 CRRDSDCPGACICRGNGYCG 72

[Close]

BLAST of CSPI04G00340 vs. NCBI nr
Match: gi|700197603|gb|KGN52761.1| (Trypsin inhibitor 1 [Cucumis sativus])

HSP 1 Score: 144.1 bits (362), Expect = 1.1e-31
Identity = 69/81 (85.19%), Postives = 73/81 (90.12%), Query Frame = 1


Query: 1  MEWKKVALVAMMGMLLMATFTESVGLGVDEEIIQLVSDGLNEYSQNIGKETV-GCPRILM 60
          MEWKK+ALVAMMGMLLMATFTESVGLGVDEEIIQLVSDG+NEYSQNIGK+T  GCPRILM
Sbjct: 1  MEWKKIALVAMMGMLLMATFTESVGLGVDEEIIQLVSDGVNEYSQNIGKDTAPGCPRILM 60

Query: 61 PCKSDHDCLSGCTCKQNGYCG 81
           CK+D DC  GCTCK  GYCG
Sbjct: 61 KCKTDSDCYPGCTCKPMGYCG 81

[Close]

BLAST of CSPI04G00340 vs. NCBI nr
Match: gi|8134511|sp|Q43667.1|ITR1_TRIKI (RecName: Full=Trypsin inhibitor 1; AltName: Full=TTII; AltName: Full=Trypsin inhibitor I; Flags: Precursor)

HSP 1 Score: 85.9 bits (211), Expect = 3.6e-14
Identity = 39/65 (60.00%), Postives = 46/65 (70.77%), Query Frame = 1


Query: 17 MATFTESVGLGVD-EEIIQLVSDGLNEYSQNIGKETVGCPRILMPCKSDHDCLSGCTCKQ 76
          MA F ES   G   +E+IQLVSDG+NEYS+ + +  V CPRILMPCK + DCL GC C  
Sbjct: 1  MAAFVESARAGAGADEVIQLVSDGVNEYSEKMMEGVVACPRILMPCKVNDDCLRGCKCLS 60

Query: 77 NGYCG 81
          NGYCG
Sbjct: 61 NGYCG 65

[Close]

BLAST of CSPI04G00340 vs. NCBI nr
Match: gi|778697386|ref|XP_011654311.1| (PREDICTED: uncharacterized protein LOC105435333 [Cucumis sativus])

HSP 1 Score: 70.5 bits (171), Expect = 1.6e-09
Identity = 33/83 (39.76%), Postives = 51/83 (61.45%), Query Frame = 1


Query: 1   MEWKKVALVAMMGMLLMATFTESVGLGVDEEIIQLVSDGLNEYS---QNIGKETVGCPRI 60
           MEWKK+ ++A++GML+MA   E     ++ E ++    GLN+     + + +ET  CPRI
Sbjct: 126 MEWKKIGMLAVVGMLVMAALVEDCHAAIESESVK----GLNKNELPRKMMNEETRMCPRI 185

Query: 61  LMPCKSDHDCLSGCTCKQNGYCG 81
           L  C +D DC++ C C  NG+CG
Sbjct: 186 LEECTTDDDCMNDCICLSNGFCG 204

[Close]

BLAST of CSPI04G00340 vs. NCBI nr
Match: gi|700197602|gb|KGN52760.1| (hypothetical protein Csa_4G000805 [Cucumis sativus])

HSP 1 Score: 70.5 bits (171), Expect = 1.6e-09
Identity = 33/83 (39.76%), Postives = 51/83 (61.45%), Query Frame = 1


Query: 1  MEWKKVALVAMMGMLLMATFTESVGLGVDEEIIQLVSDGLNEYS---QNIGKETVGCPRI 60
          MEWKK+ ++A++GML+MA   E     ++ E ++    GLN+     + + +ET  CPRI
Sbjct: 1  MEWKKIGMLAVVGMLVMAALVEDCHAAIESESVK----GLNKNELPRKMMNEETRMCPRI 60

Query: 61 LMPCKSDHDCLSGCTCKQNGYCG 81
          L  C +D DC++ C C  NG+CG
Sbjct: 61 LEECTTDDDCMNDCICLSNGFCG 79

[Close]

BLAST of CSPI04G00340 vs. NCBI nr
Match: gi|462426|sp|P34950.1|ITR5_LUFAE (RecName: Full=Trypsin inhibitor 5; AltName: Full=TGT-II; AltName: Full=Trypsin inhibitor II; Flags: Precursor)

HSP 1 Score: 67.4 bits (163), Expect = 1.3e-08
Identity = 33/66 (50.00%), Postives = 43/66 (65.15%), Query Frame = 1


Query: 17 MATFTESVGLGVDEEIIQLVSDGLNEYSQNI--GKETVGCPRILMPCKSDHDCLSGCTCK 76
          MA+  ES G+    E+I+L+SDG N+  + I  G+    CPRILMPCK+D DC+  C C 
Sbjct: 1  MASVAESSGV---VEVIELISDGGNDLPRKIMSGRHGGICPRILMPCKTDDDCMLDCRCL 60

Query: 77 QNGYCG 81
           NGYCG
Sbjct: 61 SNGYCG 63

The following BLAST results are available for this feature:

BLAST of CSPI04G00340 vs. Swiss-Prot


Match Name	E-value	Identity	Description
ITR1_TRIKI	2.2e-16	60.00	Trypsin inhibitor 1 OS=Trichosanthes kirilowii PE=3 SV=1	[more]
ITR5_LUFAE	8.2e-11	50.00	Trypsin inhibitor 5 OS=Luffa aegyptiaca PE=1 SV=1	[more]
ITR2_BRYDI	8.5e-08	75.00	Trypsin inhibitor 2 OS=Bryonia dioica PE=1 SV=1	[more]
ITR2_ECBEL	7.2e-07	71.43	Trypsin inhibitor 2 OS=Ecballium elaterium PE=1 SV=2	[more]
ITR4_LUFAE	2.1e-06	70.37	Trypsin inhibitor 4 OS=Luffa aegyptiaca PE=1 SV=1	[more]

BLAST of CSPI04G00340 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KY34_CUCSA	7.7e-32	85.19	Trypsin inhibitor 1 OS=Cucumis sativus GN=Csa_4G000810 PE=3 SV=1	[more]
A0A0A0KT01_CUCSA	1.1e-09	39.76	Uncharacterized protein OS=Cucumis sativus GN=Csa_4G000805 PE=3 SV=1	[more]
Q9S8D2_CUCME	1.0e-07	82.76	CMETI-B=TRYPSIN inhibitor OS=Cucumis melo PE=3 SV=1	[more]
A0A0A7HF87_9ROSI	5.0e-07	42.50	TIPTOP4 protein OS=Momordica subangulata GN=TIPTOP4 PE=3 SV=1	[more]
J3RCD6_MOMCO	1.1e-06	42.50	Two inhibitor peptide topologies 1 OS=Momordica cochinchinensis GN=TIPTOP1 PE=2 ...	[more]

BLAST of CSPI04G00340 vs. TAIR10


Match Name	E-value	Identity	Description

BLAST of CSPI04G00340 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700197603\|gb\|KGN52761.1\|	1.1e-31	85.19	Trypsin inhibitor 1 [Cucumis sativus]	[more]
gi\|8134511\|sp\|Q43667.1\|ITR1_TRIKI	3.6e-14	60.00	RecName: Full=Trypsin inhibitor 1; AltName: Full=TTII; AltName: Full=Trypsin inh...	[more]
gi\|778697386\|ref\|XP_011654311.1\|	1.6e-09	39.76	PREDICTED: uncharacterized protein LOC105435333 [Cucumis sativus]	[more]
gi\|700197602\|gb\|KGN52760.1\|	1.6e-09	39.76	hypothetical protein Csa_4G000805 [Cucumis sativus]	[more]
gi\|462426\|sp\|P34950.1\|ITR5_LUFAE	1.3e-08	50.00	RecName: Full=Trypsin inhibitor 5; AltName: Full=TGT-II; AltName: Full=Trypsin i...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR000737	Prot_inh_squash
IPR011052	Proteinase_amylase_inhib_sf

Vocabulary: **Molecular Function**
Term	Definition
GO:0004867	serine-type endopeptidase inhibitor activity
GO:0004866	endopeptidase inhibitor activity

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004867	serine-type endopeptidase inhibitor activity
molecular_function	GO:0004866	endopeptidase inhibitor activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI04G00340.1	CSPI04G00340.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000737	Proteinase inhibitor I7, squash	PFAM	PF00299	Squash	coord: 54..80 score: 1.9
IPR000737	Proteinase inhibitor I7, squash	PROSITE	PS00286	SQUASH_INHIBITOR	coord: 54..73 scor
IPR011052	Proteinase/amylase inhibitor domain	unknown	SSF57027	Plant inhibitors of proteinases and amylases	coord: 50..80 score: 1.08
None	No IPR available	SMART	SM00286	PTI_1	coord: 52..80 score: 1.

Synteny

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CSPI04G00340	Wild cucumber (PI 183967)	cpicpiB151
CSPI04G00340	Wild cucumber (PI 183967)	cpicpiB169
CSPI04G00340	Cucumber (Gy14) v1	cgycpiB103
CSPI04G00340	Cucumber (Gy14) v1	cgycpiB522
CSPI04G00340	Cucurbita maxima (Rimu)	cmacpiB338
CSPI04G00340	Cucurbita maxima (Rimu)	cmacpiB715
CSPI04G00340	Cucurbita moschata (Rifu)	cmocpiB329
CSPI04G00340	Cucurbita moschata (Rifu)	cmocpiB709
CSPI04G00340	Cucumber (Chinese Long) v2	cpicuB182
CSPI04G00340	Cucumber (Chinese Long) v2	cpicuB188
CSPI04G00340	Cucumber (Chinese Long) v2	cpicuB207
CSPI04G00340	Melon (DHL92) v3.5.1	cpimeB276
CSPI04G00340	Melon (DHL92) v3.5.1	cpimeB309
CSPI04G00340	Melon (DHL92) v3.5.1	cpimeB310
CSPI04G00340	Watermelon (Charleston Gray)	cpiwcgB280
CSPI04G00340	Watermelon (97103) v1	cpiwmB291
CSPI04G00340	Cucurbita pepo (Zucchini)	cpecpiB221
CSPI04G00340	Cucurbita pepo (Zucchini)	cpecpiB399
CSPI04G00340	Bottle gourd (USVL1VR-Ls)	cpilsiB274
CSPI04G00340	Melon (DHL92) v3.6.1	cpimedB301
CSPI04G00340	Cucumber (Gy14) v2	cgybcpiB168
CSPI04G00340	Cucumber (Gy14) v2	cgybcpiB179
CSPI04G00340	Silver-seed gourd	carcpiB0328
CSPI04G00340	Silver-seed gourd	carcpiB0407
CSPI04G00340	Cucumber (Chinese Long) v3	cpicucB213
CSPI04G00340	Cucumber (Chinese Long) v3	cpicucB224
CSPI04G00340	Watermelon (97103) v2	cpiwmbB278
CSPI04G00340	Watermelon (97103) v2	cpiwmbB296
CSPI04G00340	Wax gourd	cpiwgoB358