CSPI03G37280 (gene) Wild cucumber (PI 183967) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CSPI03G37280
Type	gene
Organism	Cucumis sativus (Wild cucumber (PI 183967))
Description	Steroid binding protein
Location	Chr3 : 32309305 .. 32312008 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GCTTCTTAATCAATGACCGACTCAACATTTTATTTTAAAATGTTTGGTTCAAATTAAACCTGTATAAAAATTCGAAGTTCGAGGTTTCGTTACTCACTCCATTGTTCTACTAAAAAGAAATGAAGAATTTACTAAAGGGGAGAAGTTGTTTGTTGAATAGTCAACCACGAAGCGGGTAGGTAAAGGATTCTTTCATGGTTGGTGGAGTGATTGTTCTATCTTCATAGTCAGCCCACCGGAAGTCTAATGCTTCGGCAAGATAATAGCAAGCCTCAAACGAAGAAATGGAGTTCTATTGGAAATCGGCGATGGAGCAGATCACTTGGTATACCGGACTATCCCCAACGGCCTTCTTCACCATAATTGCTGCAATGATCTTCGTGTTTCAAATGGTTTCCTCCATGTTTGTTTCTCCAGAAGAGTTTAATAAGCCCCCAACTGTTGCGATTTCTTCGTCTAACTCATCCAACTCCAATCTGTTTGTCAATGATTCGGGTGCAGACGCCTCGCAAGCAGTCCAAGTCGGGCGATTGACGGAGCAGCAGTTGAGGGCTTACAATGGTTCTGATCCGAATAAGCCACTTCTTATGGCTATAAAGGGTCAAATCTACGATGTTTCTTCGGGCAGGTACTTTTTTTCTTCCTTTATTGTTCGTTGGAGTGTACTTTTGTTTGGTCTCTGGGGATTATTTACGAACTTGACTATATTGAATAGGAGTAGTTGCTTAGTTTCTGGAGTTGCAGCTTGAATCAATGTTCTAGATGGGTAATTACTTCTTTTAATATCATCAACGGAGTTTAGGAACTGCACAATAATAACGATAACCGACCGATCAGTGTAATTTGTTCAGGGATGCATCAATCCCTTTACACTACTGTCCACCTGCAACTTTTCATATGGTCATCTGTTAAGTATTCGTATAGGAAAAATTAGAAACCATCATTATAAGATTTTTGAGCTGATGCCAATTAATTTTGAGATTGAACCCATGTCATCTCATTTGGTAGTAGAGCCTTTGAAGCCTTAAATGGGTATTTGGTCCACAAAAAGGAATTGGGATATGATTTAAAAGTGATGAGCCACATCTTGAGGGGGATATCATGTAAGGCACTTTCTAATATCTGTTCCACGTCAAGGTTTCACTGGTAATGTCTTGAACGATTTAAGATGTTTGCCTGAAATTTTTAAGAAGCAAATGTTTTTGCACGAAATATGTATGTGAAAGGAGTGGCAGGCCTTCCATTCTCTTTACTTATGTGAGAAGAAGGGTGGTAGGGACTAAAGAATGAAAGAGAATGGAAGGTGGGCTGAATGGGAGTGTGTGTTAATTGTAGACAAATTTTAAGTCAGTCAATTGGTCTGTCAGGGAATATTAAAGTGAAGCAGTTTAAGAACATTTGGGGTTCGGAAGCCTCCCCATATTTATTTAATATATGCCTGGATTTCTCAAGTGAATGAATTATAGAGGATAGCATATAGCATAAAAAATGGGTTTCTTTAGCTGAGCTTAATATAGCCTATGAAAGTAGTGAGTTCGTGGATTGAAATAAGCAAACACAGATATCAAGTATAGACTTGCTTCTTACCCTCGCAGAAGTTATTTTGGGGCGGGGTAAATGGAGTGATGGTTGGTGTTTGATATAACTTTTATAGAATTTGGGTTATTATTTCGTTAGCTTACATACTTTGTTGTTGTTTCGTTCCTATGCTTTCACTAGCCTCTAAATAGTACCTTTTGTAAGTTCCATGTCAGTGGGTGTCCAAACCAGTTTCCCTTGCAACTAATTTTACCAAGCAACAATTTCACTTGAGCCATAATTTTAACAAGACCGCCTGCTATTAACATCCCAACCCAACTAAATCTCTCAAATCTAGTTTGTTCGTTATTACCTGTTCAATTTTGATCCATCCGATTGACTTTCCATGTACACGACCTTAATAACTGTTTTCAAGAGCAGAGACTTGAGTCACTTGACAATCCTAATTCTCTACGTAATTGAAGCTTCTTAGATTTCAAAGAATAAATACAGCCCAAATTTACATGAATGAAATATGTAACTTGACCTTAGTATTAAAGTTGTCGTTGATTTTTGGTTGGCTTGATGGTCAGGATGTTTTACGGTCCTGGTTCACCGTACTCTATGTTCGTCGGAAAGGATGCCAGCCGAGCCTTGGCTCTCTTATCTTTCAAACCTGAGGACATCAATGGTAACATCGAAGGTCTGAATGAAGAAGAGCTTGTAATCTTGCAGGACTGGGAATACAAGTTCATGGAAAAGTATGTAAAAGTTGGGGAGCTTGTTCCAGAGGGAGAATTGAATGAGCATTCTGAAAATGGACACCAAAATTCCGAAACCACGCAAGAAGAACACGACAAAGATCAGTAAGAAGATGTTTATAATTTCCATCTTTTGATCAGGTAGATCTCAGAAGATTCATTCTAAATCTCATGATGCTGTTGAGTGTGCAAGTCCTTTGTCTTGTTCCTCCTGATCTAGTGAAGCTGTGGCCTCCTTTGAGCATACATAAAAAATTGCAAGTAGAAGAAATCAATCTGATTGAAGTTAGAAATTCTGCTACCTTTGGGCTAGACTTTTGCCTGTTTTGTTTGTATATTGTTTTGAGACTAGACTTTTATAACACTAATGAGTATATTATTTTATTTCATAGGTTTTACCCATAAATGTAAATGTTCTTCATGCG

mRNA sequence

ATGGAGTTCTATTGGAAATCGGCGATGGAGCAGATCACTTGGTATACCGGACTATCCCCAACGGCCTTCTTCACCATAATTGCTGCAATGATCTTCGTGTTTCAAATGGTTTCCTCCATGTTTGTTTCTCCAGAAGAGTTTAATAAGCCCCCAACTGTTGCGATTTCTTCGTCTAACTCATCCAACTCCAATCTGTTTGTCAATGATTCGGGTGCAGACGCCTCGCAAGCAGTCCAAGTCGGGCGATTGACGGAGCAGCAGTTGAGGGCTTACAATGGTTCTGATCCGAATAAGCCACTTCTTATGGCTATAAAGGGTCAAATCTACGATGTTTCTTCGGGCAGGATGTTTTACGGTCCTGGTTCACCGTACTCTATGTTCGTCGGAAAGGATGCCAGCCGAGCCTTGGCTCTCTTATCTTTCAAACCTGAGGACATCAATGGTAACATCGAAGGTCTGAATGAAGAAGAGCTTGTAATCTTGCAGGACTGGGAATACAAGTTCATGGAAAAGTATGTAAAAGTTGGGGAGCTTGTTCCAGAGGGAGAATTGAATGAGCATTCTGAAAATGGACACCAAAATTCCGAAACCACGCAAGAAGAACACGACAAAGATCAGTAA

Coding sequence (CDS)

ATGGAGTTCTATTGGAAATCGGCGATGGAGCAGATCACTTGGTATACCGGACTATCCCCAACGGCCTTCTTCACCATAATTGCTGCAATGATCTTCGTGTTTCAAATGGTTTCCTCCATGTTTGTTTCTCCAGAAGAGTTTAATAAGCCCCCAACTGTTGCGATTTCTTCGTCTAACTCATCCAACTCCAATCTGTTTGTCAATGATTCGGGTGCAGACGCCTCGCAAGCAGTCCAAGTCGGGCGATTGACGGAGCAGCAGTTGAGGGCTTACAATGGTTCTGATCCGAATAAGCCACTTCTTATGGCTATAAAGGGTCAAATCTACGATGTTTCTTCGGGCAGGATGTTTTACGGTCCTGGTTCACCGTACTCTATGTTCGTCGGAAAGGATGCCAGCCGAGCCTTGGCTCTCTTATCTTTCAAACCTGAGGACATCAATGGTAACATCGAAGGTCTGAATGAAGAAGAGCTTGTAATCTTGCAGGACTGGGAATACAAGTTCATGGAAAAGTATGTAAAAGTTGGGGAGCTTGTTCCAGAGGGAGAATTGAATGAGCATTCTGAAAATGGACACCAAAATTCCGAAACCACGCAAGAAGAACACGACAAAGATCAGTAA

Homology

[Close]

BLAST of CSPI03G37280 vs. Swiss-Prot
Match: MSBP2_ARATH (Membrane steroid-binding protein 2 OS=Arabidopsis thaliana GN=MSBP2 PE=1 SV=1)

HSP 1 Score: 183.0 bits (463), Expect = 3.4e-45
Identity = 100/186 (53.76%), Postives = 124/186 (66.67%), Query Frame = 1


Query: 5   WKSAMEQITWYTGLSPTAFFTIIAAMIFVFQMVSSMFVSPEEFNKPPTVAISSSNSSNSN 64
           W++  E IT YTGLSP AFFT++A    V+Q+VS  FVSPE  ++P             +
Sbjct: 6   WETLKETITAYTGLSPAAFFTVLALAFAVYQVVSGFFVSPE-VHRP------------RS 65

Query: 65  LFVNDSGADASQAVQVGRLTEQQLRAYNGSDPNKPLLMAIKGQIYDVSSGRMFYGPGSPY 124
           L V          VQ+G +TE++L+ Y+GSD  KPLLMAIKGQIYDVS  RMFYGPG PY
Sbjct: 66  LEVQPQSEPLPPPVQLGEITEEELKLYDGSDSKKPLLMAIKGQIYDVSQSRMFYGPGGPY 125

Query: 125 SMFVGKDASRALALLSFKPEDINGNIEGLNEEELVILQDWEYKFMEKYVKVGEL-VPEGE 184
           ++F GKDASRALA +SF+ +D+ G+I GL   EL  LQDWEYKFM KYVKVG +   +GE
Sbjct: 126 ALFAGKDASRALAKMSFEDQDLTGDISGLGAFELEALQDWEYKFMSKYVKVGTIQKKDGE 178

Query: 185 LNEHSE 190
             E SE
Sbjct: 186 GKESSE 178

[Close]

BLAST of CSPI03G37280 vs. Swiss-Prot
Match: MSBP1_ARATH (Membrane steroid-binding protein 1 OS=Arabidopsis thaliana GN=MSBP1 PE=1 SV=2)

HSP 1 Score: 165.6 bits (418), Expect = 5.7e-40
Identity = 90/200 (45.00%), Postives = 121/200 (60.50%), Query Frame = 1


Query: 5   WKSAMEQITWYTGLSPTAFFTIIAAMIFVFQMVSSMFVSPEEFNKPPTVAISSSNSSNSN 64
           W++  E I  YTGLSP  FFT +A    ++Q++S  F SP +           +    + 
Sbjct: 6   WQTLKEAIHAYTGLSPVVFFTALALAFAIYQVISGWFASPFD---------DVNRHQRAR 65

Query: 65  LFVNDSGADASQAVQVGRLTEQQLRAYNGSDPNKPLLMAIKGQIYDVSSGRMFYGPGSPY 124
               +      Q VQVG +TE++L+ Y+GSDP KPLLMAIK QIYDV+  RMFYGPG PY
Sbjct: 66  SLAQEEEPPIPQPVQVGEITEEELKQYDGSDPQKPLLMAIKHQIYDVTQSRMFYGPGGPY 125

Query: 125 SMFVGKDASRALALLSFKPEDINGNIEGLNEEELVILQDWEYKFMEKYVKVGELVPEG-- 184
           ++F GKDASRALA +SF+ +D+  ++ GL   EL  LQDWEYKFM KY KVG +   G  
Sbjct: 126 ALFAGKDASRALAKMSFEEKDLTWDVSGLGPFELDALQDWEYKFMSKYAKVGTVKVAGSE 185

Query: 185 ----ELNEHSENGHQNSETT 199
                ++E +EN  Q++  T
Sbjct: 186 PETASVSEPTENVEQDAHVT 196

[Close]

BLAST of CSPI03G37280 vs. Swiss-Prot
Match: SBP3_ARATH (Probable steroid-binding protein 3 OS=Arabidopsis thaliana GN=MP3 PE=1 SV=1)

HSP 1 Score: 112.1 bits (279), Expect = 7.4e-24
Identity = 51/96 (53.12%), Postives = 70/96 (72.92%), Query Frame = 1


Query: 84  TEQQLRAYNGSDPNKPLLMAIKGQIYDVSSGRMFYGPGSPYSMFVGKDASRALALLSFKP 143
           T +QL  YNG+D +KP+ +AIKG+++DV++G+ FYG G  YSMF GKDASRAL  +S   
Sbjct: 4   TAEQLSQYNGTDESKPIYVAIKGRVFDVTTGKSFYGSGGDYSMFAGKDASRALGKMSKNE 63

Query: 144 EDINGNIEGLNEEELVILQDWEYKFMEKYVKVGELV 180
           ED++ ++EGL E+E+  L DWE KF  KY  VG +V
Sbjct: 64  EDVSPSLEGLTEKEINTLNDWETKFEAKYPVVGRVV 99

[Close]

BLAST of CSPI03G37280 vs. Swiss-Prot
Match: NEUFC_DANRE (Neuferricin OS=Danio rerio GN=cyb5d2 PE=3 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 4.4e-16
Identity = 40/97 (41.24%), Postives = 56/97 (57.73%), Query Frame = 1


Query: 83  LTEQQLRAYNGSDPNKPLLMAIKGQIYDVSSGRMFYGPGSPYSMFVGKDASRALALLSFK 142
           LT++QL  YNG   +K L +AI GQ++DV  GR  YGPG  Y  F GKDASRA     F 
Sbjct: 54  LTKEQLSLYNGGKNSKGLYLAILGQVFDVEKGRKHYGPGGGYHFFTGKDASRAFITGDFT 113

Query: 143 PEDINGNIEGLNEEELVILQDWEYKFMEKYVKVGELV 180
              ++ ++   +E ++V L DW   +   Y  VG+L+
Sbjct: 114 EAGLSNDVSDFSESQIVALYDWLSFYQRDYTPVGKLI 150

[Close]

BLAST of CSPI03G37280 vs. Swiss-Prot
Match: NENF_RAT (Neudesin OS=Rattus norvegicus GN=Nenf PE=2 SV=1)

HSP 1 Score: 85.5 bits (210), Expect = 7.5e-16
Identity = 41/104 (39.42%), Postives = 65/104 (62.50%), Query Frame = 1


Query: 74  ASQAVQVGRLTEQQLRAYNGSDPNKPLLMAIKGQIYDVSSGRMFYGPGSPYSMFVGKDAS 133
           A +   V   TE++L  Y+G + ++P+ +A+KG ++DV+SG+ FYG G+PY+   GKD+S
Sbjct: 37  AERGPPVRLFTEEELARYSGEEEDQPIYLAVKGVVFDVTSGKEFYGRGAPYNALAGKDSS 96

Query: 134 RALALLSFKPEDINGNIEGLNEEELVILQD-WEYKFMEKYVKVG 177
           R +A +S  P D+  +I GL  +EL  L D +   +  KY  VG
Sbjct: 97  RGVAKMSLDPADLTHDISGLTAKELEALDDIFSKVYKAKYPIVG 140

[Close]

BLAST of CSPI03G37280 vs. TrEMBL
Match: A0A0A0LC44_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G815470 PE=3 SV=1)

HSP 1 Score: 400.2 bits (1027), Expect = 1.5e-108
Identity = 204/206 (99.03%), Postives = 205/206 (99.51%), Query Frame = 1


Query: 1   MEFYWKSAMEQITWYTGLSPTAFFTIIAAMIFVFQMVSSMFVSPEEFNKPPTVAISSSNS 60
           MEFYWKSAMEQITWYTGLSPTAFFTI+AAMIFVFQMVSSMFVSPEEFNKPPTVAISSSNS
Sbjct: 1   MEFYWKSAMEQITWYTGLSPTAFFTILAAMIFVFQMVSSMFVSPEEFNKPPTVAISSSNS 60

Query: 61  SNSNLFVNDSGADASQAVQVGRLTEQQLRAYNGSDPNKPLLMAIKGQIYDVSSGRMFYGP 120
           SNSNLFVNDSGADAS AVQVGRLTEQQLRAYNGSDPNKPLLMAIKGQIYDVSSGRMFYGP
Sbjct: 61  SNSNLFVNDSGADASLAVQVGRLTEQQLRAYNGSDPNKPLLMAIKGQIYDVSSGRMFYGP 120

Query: 121 GSPYSMFVGKDASRALALLSFKPEDINGNIEGLNEEELVILQDWEYKFMEKYVKVGELVP 180
           GSPYSMFVGKDASRALALLSFKPEDINGNIEGLNEEELVILQDWEYKFMEKYVKVGELVP
Sbjct: 121 GSPYSMFVGKDASRALALLSFKPEDINGNIEGLNEEELVILQDWEYKFMEKYVKVGELVP 180

Query: 181 EGELNEHSENGHQNSETTQEEHDKDQ 207
           EGELNEHSENGHQNSETTQEEHDKDQ
Sbjct: 181 EGELNEHSENGHQNSETTQEEHDKDQ 206

[Close]

BLAST of CSPI03G37280 vs. TrEMBL
Match: E5GBX8_CUCME (Steroid binding protein OS=Cucumis melo subsp. melo PE=3 SV=1)

HSP 1 Score: 388.7 bits (997), Expect = 4.6e-105
Identity = 198/206 (96.12%), Postives = 202/206 (98.06%), Query Frame = 1


Query: 1   MEFYWKSAMEQITWYTGLSPTAFFTIIAAMIFVFQMVSSMFVSPEEFNKPPTVAISSSNS 60
           MEFYW+SAMEQITWYTGLSPTAFFTI+AAMIFVFQMVSSMFVSPEEFNKPPTV ISSSNS
Sbjct: 1   MEFYWRSAMEQITWYTGLSPTAFFTILAAMIFVFQMVSSMFVSPEEFNKPPTVPISSSNS 60

Query: 61  SNSNLFVNDSGADASQAVQVGRLTEQQLRAYNGSDPNKPLLMAIKGQIYDVSSGRMFYGP 120
           SNSNLFVNDS ADASQAV VGRLTEQQLRAYNGSDPNKPLLMAIKGQIYDVSSGR+FYGP
Sbjct: 61  SNSNLFVNDSAADASQAVLVGRLTEQQLRAYNGSDPNKPLLMAIKGQIYDVSSGRIFYGP 120

Query: 121 GSPYSMFVGKDASRALALLSFKPEDINGNIEGLNEEELVILQDWEYKFMEKYVKVGELVP 180
           GSPYSMFVGKDASRALALLSFKPEDINGNIEGL+EEELVILQDWEYKFMEKYVKVGELVP
Sbjct: 121 GSPYSMFVGKDASRALALLSFKPEDINGNIEGLSEEELVILQDWEYKFMEKYVKVGELVP 180

Query: 181 EGELNEHSENGHQNSETTQEEHDKDQ 207
           EGELNEHSENGHQNSETTQ EHDKDQ
Sbjct: 181 EGELNEHSENGHQNSETTQVEHDKDQ 206

[Close]

BLAST of CSPI03G37280 vs. TrEMBL
Match: W9S7V8_9ROSA (Membrane steroid-binding protein 2 OS=Morus notabilis GN=L484_027995 PE=3 SV=1)

HSP 1 Score: 235.3 bits (599), Expect = 6.5e-59
Identity = 124/180 (68.89%), Postives = 146/180 (81.11%), Query Frame = 1


Query: 5   WKSAMEQITWYTGLSPTAFFTIIAAMIFVFQMVSSMFVSPEEFNKPPTVAISSSNSSNSN 64
           + +A+E+ITWYTGLSP AFFTI A M+ V++ VSSMFVSPE+FNKPP VA+S    S SN
Sbjct: 4   YSAAVEEITWYTGLSPAAFFTIAALMVVVYKTVSSMFVSPEDFNKPP-VAMSIEKISQSN 63

Query: 65  L-FVNDSGADA--SQAVQVGRLTEQQLRAYNGSDPNKPLLMAIKGQIYDVSSGRMFYGPG 124
             F+N+  A    SQ +Q+G +TEQQLRAYNGSDPNKPLL++IKGQIYDVSS RMFYGPG
Sbjct: 64  SDFLNNVMATTTNSQPLQLGDMTEQQLRAYNGSDPNKPLLISIKGQIYDVSSSRMFYGPG 123

Query: 125 SPYSMFVGKDASRALALLSFKPEDINGNIEGLNEEELVILQDWEYKFMEKYVKVGELVPE 182
             YSMF GK+ASRALALLSFKP+DINGN+E L  EEL +L+DWE KF EKY KVG+LV E
Sbjct: 124 GAYSMFTGKEASRALALLSFKPQDINGNLEDLGPEELQVLEDWEDKFTEKYAKVGQLVRE 182

[Close]

BLAST of CSPI03G37280 vs. TrEMBL
Match: V7C281_PHAVU (Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_004G050900g PE=3 SV=1)

HSP 1 Score: 229.9 bits (585), Expect = 2.7e-57
Identity = 120/203 (59.11%), Postives = 153/203 (75.37%), Query Frame = 1


Query: 4   YWKSAMEQITWYTGLSPTAFFTIIAAMIFVFQMVSSMFVSPEEFNKPPTVAISSSNSSNS 63
           ++ S ME+IT+YTGLSPTAFFT++A M+ V++ VSSMFVSPE++NKPP V+  +      
Sbjct: 3   FYTSLMEEITFYTGLSPTAFFTVLAMMVVVYRTVSSMFVSPEDYNKPPVVSARAHPQF-- 62

Query: 64  NLFVNDSGADASQAVQVGRLTEQQLRAYNGSDPNKPLLMAIKGQIYDVSSGRMFYGPGSP 123
                D      Q VQ+G++T+++LRAY+GSDPNKPLLMAIKGQIYDVS+GR FYGPG P
Sbjct: 63  -----DHPEPPRQPVQLGQVTDRELRAYDGSDPNKPLLMAIKGQIYDVSTGRNFYGPGGP 122

Query: 124 YSMFVGKDASRALALLSFKPEDINGNIEGLNEEELVILQDWEYKFMEKYVKVGELVPE-- 183
           Y+MF GK+ SRAL+LLSFKPEDING++EGL E EL IL+DWE+KF+EKY KVG L+PE  
Sbjct: 123 YAMFAGKECSRALSLLSFKPEDINGDLEGLGESELTILEDWEFKFIEKYPKVGVLIPEQR 182

Query: 184 GELNEHSENGHQNSETTQEEHDK 205
            + NE +E    N E   E  D+
Sbjct: 183 TQQNELTEQVQDNLENPNEYKDE 198

[Close]

BLAST of CSPI03G37280 vs. TrEMBL
Match: K7MWY8_SOYBN (Uncharacterized protein OS=Glycine max PE=3 SV=1)

HSP 1 Score: 228.8 bits (582), Expect = 6.1e-57
Identity = 119/202 (58.91%), Postives = 152/202 (75.25%), Query Frame = 1


Query: 5   WKSAMEQITWYTGLSPTAFFTIIAAMIFVFQMVSSMFVSPEEFNKPPTVAISSSNSSNSN 64
           + S ME+I++YTGLSP AFFTI+A M+ V++ VS MFVSPE++NKPP V    S  +NS 
Sbjct: 71  YTSVMEEISFYTGLSPAAFFTILAMMVVVYRTVSGMFVSPEDYNKPPVV----SARANSR 130

Query: 65  LFVNDSGADASQAVQVGRLTEQQLRAYNGSDPNKPLLMAIKGQIYDVSSGRMFYGPGSPY 124
           L   D      + VQ+G +T+++LRAY+GSDP+KPLLMAIKGQIYDVS+GR FYGPG PY
Sbjct: 131 L---DESEPPREPVQLGEITDRELRAYDGSDPSKPLLMAIKGQIYDVSNGRNFYGPGGPY 190

Query: 125 SMFVGKDASRALALLSFKPEDINGNIEGLNEEELVILQDWEYKFMEKYVKVGELVPE--- 184
           +MF GK+ SRALALLSFKP+DINGN+EGL EEEL IL+DWE+KF+EKY KVG+L+ E   
Sbjct: 191 AMFAGKECSRALALLSFKPDDINGNLEGLGEEELTILEDWEFKFIEKYPKVGQLIAEQRT 250

Query: 185 --GELNEHSENGHQNSETTQEE 202
              E  E +++   N    +EE
Sbjct: 251 RQNEFKEQTQDNLDNPNECKEE 265

[Close]

BLAST of CSPI03G37280 vs. TAIR10
Match: AT3G48890.1 (AT3G48890.1 membrane-associated progesterone binding protein 3)

HSP 1 Score: 183.0 bits (463), Expect = 1.9e-46
Identity = 100/186 (53.76%), Postives = 124/186 (66.67%), Query Frame = 1


Query: 5   WKSAMEQITWYTGLSPTAFFTIIAAMIFVFQMVSSMFVSPEEFNKPPTVAISSSNSSNSN 64
           W++  E IT YTGLSP AFFT++A    V+Q+VS  FVSPE  ++P             +
Sbjct: 6   WETLKETITAYTGLSPAAFFTVLALAFAVYQVVSGFFVSPE-VHRP------------RS 65

Query: 65  LFVNDSGADASQAVQVGRLTEQQLRAYNGSDPNKPLLMAIKGQIYDVSSGRMFYGPGSPY 124
           L V          VQ+G +TE++L+ Y+GSD  KPLLMAIKGQIYDVS  RMFYGPG PY
Sbjct: 66  LEVQPQSEPLPPPVQLGEITEEELKLYDGSDSKKPLLMAIKGQIYDVSQSRMFYGPGGPY 125

Query: 125 SMFVGKDASRALALLSFKPEDINGNIEGLNEEELVILQDWEYKFMEKYVKVGEL-VPEGE 184
           ++F GKDASRALA +SF+ +D+ G+I GL   EL  LQDWEYKFM KYVKVG +   +GE
Sbjct: 126 ALFAGKDASRALAKMSFEDQDLTGDISGLGAFELEALQDWEYKFMSKYVKVGTIQKKDGE 178

Query: 185 LNEHSE 190
             E SE
Sbjct: 186 GKESSE 178

[Close]

BLAST of CSPI03G37280 vs. TAIR10
Match: AT5G52240.1 (AT5G52240.1 membrane steroid binding protein 1)

HSP 1 Score: 165.6 bits (418), Expect = 3.2e-41
Identity = 90/200 (45.00%), Postives = 121/200 (60.50%), Query Frame = 1


Query: 5   WKSAMEQITWYTGLSPTAFFTIIAAMIFVFQMVSSMFVSPEEFNKPPTVAISSSNSSNSN 64
           W++  E I  YTGLSP  FFT +A    ++Q++S  F SP +           +    + 
Sbjct: 6   WQTLKEAIHAYTGLSPVVFFTALALAFAIYQVISGWFASPFD---------DVNRHQRAR 65

Query: 65  LFVNDSGADASQAVQVGRLTEQQLRAYNGSDPNKPLLMAIKGQIYDVSSGRMFYGPGSPY 124
               +      Q VQVG +TE++L+ Y+GSDP KPLLMAIK QIYDV+  RMFYGPG PY
Sbjct: 66  SLAQEEEPPIPQPVQVGEITEEELKQYDGSDPQKPLLMAIKHQIYDVTQSRMFYGPGGPY 125

Query: 125 SMFVGKDASRALALLSFKPEDINGNIEGLNEEELVILQDWEYKFMEKYVKVGELVPEG-- 184
           ++F GKDASRALA +SF+ +D+  ++ GL   EL  LQDWEYKFM KY KVG +   G  
Sbjct: 126 ALFAGKDASRALAKMSFEEKDLTWDVSGLGPFELDALQDWEYKFMSKYAKVGTVKVAGSE 185

Query: 185 ----ELNEHSENGHQNSETT 199
                ++E +EN  Q++  T
Sbjct: 186 PETASVSEPTENVEQDAHVT 196

[Close]

BLAST of CSPI03G37280 vs. TAIR10
Match: AT2G24940.1 (AT2G24940.1 membrane-associated progesterone binding protein 2)

HSP 1 Score: 112.1 bits (279), Expect = 4.2e-25
Identity = 51/96 (53.12%), Postives = 70/96 (72.92%), Query Frame = 1


Query: 84  TEQQLRAYNGSDPNKPLLMAIKGQIYDVSSGRMFYGPGSPYSMFVGKDASRALALLSFKP 143
           T +QL  YNG+D +KP+ +AIKG+++DV++G+ FYG G  YSMF GKDASRAL  +S   
Sbjct: 4   TAEQLSQYNGTDESKPIYVAIKGRVFDVTTGKSFYGSGGDYSMFAGKDASRALGKMSKNE 63

Query: 144 EDINGNIEGLNEEELVILQDWEYKFMEKYVKVGELV 180
           ED++ ++EGL E+E+  L DWE KF  KY  VG +V
Sbjct: 64  EDVSPSLEGLTEKEINTLNDWETKFEAKYPVVGRVV 99

[Close]

BLAST of CSPI03G37280 vs. TAIR10
Match: AT4G14965.1 (AT4G14965.1 membrane-associated progesterone binding protein 4)

HSP 1 Score: 79.3 bits (194), Expect = 3.0e-15
Identity = 37/116 (31.90%), Postives = 62/116 (53.45%), Query Frame = 1


Query: 64  NLFVNDSGADASQAVQVGRLTEQQLRAYNGSDPNKPLLMAIKGQIYDVSSGRMFYGPGSP 123
           +L+   S        Q    + ++L  YNG+D   P+L+ I G ++DV+ G+  YG G  
Sbjct: 23  SLYFRSSFKSPQHQYQKRLFSAEELALYNGTDETLPILLGILGSVFDVTKGKFHYGSGGG 82

Query: 124 YSMFVGKDASRALALLSFKPEDINGNIEGLNEEELVILQDWEYKFMEKYVKVGELV 180
           Y+ F G+DASRA    +F  + +  +++GL+  E+  + DW   +   Y  VG+LV
Sbjct: 83  YNHFAGRDASRAFVSGNFTGDGLTDSLQGLSSSEVKSIVDWRGFYSRTYTPVGKLV 138

[Close]

BLAST of CSPI03G37280 vs. NCBI nr
Match: gi|449437656|ref|XP_004136607.1| (PREDICTED: membrane steroid-binding protein 2-like [Cucumis sativus])

HSP 1 Score: 400.2 bits (1027), Expect = 2.2e-108
Identity = 204/206 (99.03%), Postives = 205/206 (99.51%), Query Frame = 1


Query: 1   MEFYWKSAMEQITWYTGLSPTAFFTIIAAMIFVFQMVSSMFVSPEEFNKPPTVAISSSNS 60
           MEFYWKSAMEQITWYTGLSPTAFFTI+AAMIFVFQMVSSMFVSPEEFNKPPTVAISSSNS
Sbjct: 1   MEFYWKSAMEQITWYTGLSPTAFFTILAAMIFVFQMVSSMFVSPEEFNKPPTVAISSSNS 60

Query: 61  SNSNLFVNDSGADASQAVQVGRLTEQQLRAYNGSDPNKPLLMAIKGQIYDVSSGRMFYGP 120
           SNSNLFVNDSGADAS AVQVGRLTEQQLRAYNGSDPNKPLLMAIKGQIYDVSSGRMFYGP
Sbjct: 61  SNSNLFVNDSGADASLAVQVGRLTEQQLRAYNGSDPNKPLLMAIKGQIYDVSSGRMFYGP 120

Query: 121 GSPYSMFVGKDASRALALLSFKPEDINGNIEGLNEEELVILQDWEYKFMEKYVKVGELVP 180
           GSPYSMFVGKDASRALALLSFKPEDINGNIEGLNEEELVILQDWEYKFMEKYVKVGELVP
Sbjct: 121 GSPYSMFVGKDASRALALLSFKPEDINGNIEGLNEEELVILQDWEYKFMEKYVKVGELVP 180

Query: 181 EGELNEHSENGHQNSETTQEEHDKDQ 207
           EGELNEHSENGHQNSETTQEEHDKDQ
Sbjct: 181 EGELNEHSENGHQNSETTQEEHDKDQ 206

[Close]

BLAST of CSPI03G37280 vs. NCBI nr
Match: gi|659084981|ref|XP_008443179.1| (PREDICTED: membrane steroid-binding protein 2-like [Cucumis melo])

HSP 1 Score: 388.7 bits (997), Expect = 6.6e-105
Identity = 198/206 (96.12%), Postives = 202/206 (98.06%), Query Frame = 1


Query: 1   MEFYWKSAMEQITWYTGLSPTAFFTIIAAMIFVFQMVSSMFVSPEEFNKPPTVAISSSNS 60
           MEFYW+SAMEQITWYTGLSPTAFFTI+AAMIFVFQMVSSMFVSPEEFNKPPTV ISSSNS
Sbjct: 1   MEFYWRSAMEQITWYTGLSPTAFFTILAAMIFVFQMVSSMFVSPEEFNKPPTVPISSSNS 60

Query: 61  SNSNLFVNDSGADASQAVQVGRLTEQQLRAYNGSDPNKPLLMAIKGQIYDVSSGRMFYGP 120
           SNSNLFVNDS ADASQAV VGRLTEQQLRAYNGSDPNKPLLMAIKGQIYDVSSGR+FYGP
Sbjct: 61  SNSNLFVNDSAADASQAVLVGRLTEQQLRAYNGSDPNKPLLMAIKGQIYDVSSGRIFYGP 120

Query: 121 GSPYSMFVGKDASRALALLSFKPEDINGNIEGLNEEELVILQDWEYKFMEKYVKVGELVP 180
           GSPYSMFVGKDASRALALLSFKPEDINGNIEGL+EEELVILQDWEYKFMEKYVKVGELVP
Sbjct: 121 GSPYSMFVGKDASRALALLSFKPEDINGNIEGLSEEELVILQDWEYKFMEKYVKVGELVP 180

Query: 181 EGELNEHSENGHQNSETTQEEHDKDQ 207
           EGELNEHSENGHQNSETTQ EHDKDQ
Sbjct: 181 EGELNEHSENGHQNSETTQVEHDKDQ 206

[Close]

BLAST of CSPI03G37280 vs. NCBI nr
Match: gi|703155952|ref|XP_010111338.1| (Membrane steroid-binding protein 2 [Morus notabilis])

HSP 1 Score: 235.3 bits (599), Expect = 9.3e-59
Identity = 124/180 (68.89%), Postives = 146/180 (81.11%), Query Frame = 1


Query: 5   WKSAMEQITWYTGLSPTAFFTIIAAMIFVFQMVSSMFVSPEEFNKPPTVAISSSNSSNSN 64
           + +A+E+ITWYTGLSP AFFTI A M+ V++ VSSMFVSPE+FNKPP VA+S    S SN
Sbjct: 4   YSAAVEEITWYTGLSPAAFFTIAALMVVVYKTVSSMFVSPEDFNKPP-VAMSIEKISQSN 63

Query: 65  L-FVNDSGADA--SQAVQVGRLTEQQLRAYNGSDPNKPLLMAIKGQIYDVSSGRMFYGPG 124
             F+N+  A    SQ +Q+G +TEQQLRAYNGSDPNKPLL++IKGQIYDVSS RMFYGPG
Sbjct: 64  SDFLNNVMATTTNSQPLQLGDMTEQQLRAYNGSDPNKPLLISIKGQIYDVSSSRMFYGPG 123

Query: 125 SPYSMFVGKDASRALALLSFKPEDINGNIEGLNEEELVILQDWEYKFMEKYVKVGELVPE 182
             YSMF GK+ASRALALLSFKP+DINGN+E L  EEL +L+DWE KF EKY KVG+LV E
Sbjct: 124 GAYSMFTGKEASRALALLSFKPQDINGNLEDLGPEELQVLEDWEDKFTEKYAKVGQLVRE 182

[Close]

BLAST of CSPI03G37280 vs. NCBI nr
Match: gi|1021552627|ref|XP_016167631.1| (PREDICTED: membrane steroid-binding protein 2-like [Arachis ipaensis])

HSP 1 Score: 230.7 bits (587), Expect = 2.3e-57
Identity = 118/202 (58.42%), Postives = 150/202 (74.26%), Query Frame = 1


Query: 4   YWKSAMEQITWYTGLSPTAFFTIIAAMIFVFQMVSSMFVSPEEFNKPPTVAISSSNSSNS 63
           ++ + M++ITWYTGLSPTAFFTI A M+ V++ +S MFVSPE++NKPP ++  +S+    
Sbjct: 3   FYSTVMDEITWYTGLSPTAFFTIAAMMLVVYKTISGMFVSPEDYNKPPVISGRTSSRF-- 62

Query: 64  NLFVNDSGADASQAVQVGRLTEQQLRAYNGSDPNKPLLMAIKGQIYDVSSGRMFYGPGSP 123
                D+     + VQ+G +TEQ+LR YNGSDP KPLL+AIKGQIYDVSSGR FYGPG  
Sbjct: 63  -----DNTEPQREPVQLGEITEQELRQYNGSDPAKPLLIAIKGQIYDVSSGRNFYGPGGA 122

Query: 124 YSMFVGKDASRALALLSFKPEDINGNIEGLNEEELVILQDWEYKFMEKYVKVGELVP--- 183
           Y+MF GK+ SRALALLSFKP+DINGN+EGL+E EL IL+DWEYKF+EKY KVG+LVP   
Sbjct: 123 YAMFTGKECSRALALLSFKPDDINGNLEGLDESELAILEDWEYKFIEKYPKVGQLVPGQR 182

Query: 184 --EGELNEHSENGHQNSETTQE 201
             + E NE  +N     E  +E
Sbjct: 183 PLQNEHNEQVQNNLNLDEAKEE 197

[Close]

BLAST of CSPI03G37280 vs. NCBI nr
Match: gi|593702159|ref|XP_007151489.1| (hypothetical protein PHAVU_004G050900g [Phaseolus vulgaris])

HSP 1 Score: 229.9 bits (585), Expect = 3.9e-57
Identity = 120/203 (59.11%), Postives = 153/203 (75.37%), Query Frame = 1


Query: 4   YWKSAMEQITWYTGLSPTAFFTIIAAMIFVFQMVSSMFVSPEEFNKPPTVAISSSNSSNS 63
           ++ S ME+IT+YTGLSPTAFFT++A M+ V++ VSSMFVSPE++NKPP V+  +      
Sbjct: 3   FYTSLMEEITFYTGLSPTAFFTVLAMMVVVYRTVSSMFVSPEDYNKPPVVSARAHPQF-- 62

Query: 64  NLFVNDSGADASQAVQVGRLTEQQLRAYNGSDPNKPLLMAIKGQIYDVSSGRMFYGPGSP 123
                D      Q VQ+G++T+++LRAY+GSDPNKPLLMAIKGQIYDVS+GR FYGPG P
Sbjct: 63  -----DHPEPPRQPVQLGQVTDRELRAYDGSDPNKPLLMAIKGQIYDVSTGRNFYGPGGP 122

Query: 124 YSMFVGKDASRALALLSFKPEDINGNIEGLNEEELVILQDWEYKFMEKYVKVGELVPE-- 183
           Y+MF GK+ SRAL+LLSFKPEDING++EGL E EL IL+DWE+KF+EKY KVG L+PE  
Sbjct: 123 YAMFAGKECSRALSLLSFKPEDINGDLEGLGESELTILEDWEFKFIEKYPKVGVLIPEQR 182

Query: 184 GELNEHSENGHQNSETTQEEHDK 205
            + NE +E    N E   E  D+
Sbjct: 183 TQQNELTEQVQDNLENPNEYKDE 198

The following BLAST results are available for this feature:

BLAST of CSPI03G37280 vs. Swiss-Prot


Match Name	E-value	Identity	Description
MSBP2_ARATH	3.4e-45	53.76	Membrane steroid-binding protein 2 OS=Arabidopsis thaliana GN=MSBP2 PE=1 SV=1	[more]
MSBP1_ARATH	5.7e-40	45.00	Membrane steroid-binding protein 1 OS=Arabidopsis thaliana GN=MSBP1 PE=1 SV=2	[more]
SBP3_ARATH	7.4e-24	53.13	Probable steroid-binding protein 3 OS=Arabidopsis thaliana GN=MP3 PE=1 SV=1	[more]
NEUFC_DANRE	4.4e-16	41.24	Neuferricin OS=Danio rerio GN=cyb5d2 PE=3 SV=1	[more]
NENF_RAT	7.5e-16	39.42	Neudesin OS=Rattus norvegicus GN=Nenf PE=2 SV=1	[more]

BLAST of CSPI03G37280 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LC44_CUCSA	1.5e-108	99.03	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G815470 PE=3 SV=1	[more]
E5GBX8_CUCME	4.6e-105	96.12	Steroid binding protein OS=Cucumis melo subsp. melo PE=3 SV=1	[more]
W9S7V8_9ROSA	6.5e-59	68.89	Membrane steroid-binding protein 2 OS=Morus notabilis GN=L484_027995 PE=3 SV=1	[more]
V7C281_PHAVU	2.7e-57	59.11	Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_004G050900g PE=3 SV=1	[more]
K7MWY8_SOYBN	6.1e-57	58.91	Uncharacterized protein OS=Glycine max PE=3 SV=1	[more]

BLAST of CSPI03G37280 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G48890.1	1.9e-46	53.76	membrane-associated progesterone binding protein 3	[more]
AT5G52240.1	3.2e-41	45.00	membrane steroid binding protein 1	[more]
AT2G24940.1	4.2e-25	53.13	membrane-associated progesterone binding protein 2	[more]
AT4G14965.1	3.0e-15	31.90	membrane-associated progesterone binding protein 4	[more]

BLAST of CSPI03G37280 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449437656\|ref\|XP_004136607.1\|	2.2e-108	99.03	PREDICTED: membrane steroid-binding protein 2-like [Cucumis sativus]	[more]
gi\|659084981\|ref\|XP_008443179.1\|	6.6e-105	96.12	PREDICTED: membrane steroid-binding protein 2-like [Cucumis melo]	[more]
gi\|703155952\|ref\|XP_010111338.1\|	9.3e-59	68.89	Membrane steroid-binding protein 2 [Morus notabilis]	[more]
gi\|1021552627\|ref\|XP_016167631.1\|	2.3e-57	58.42	PREDICTED: membrane steroid-binding protein 2-like [Arachis ipaensis]	[more]
gi\|593702159\|ref\|XP_007151489.1\|	3.9e-57	59.11	hypothetical protein PHAVU_004G050900g [Phaseolus vulgaris]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR001199	Cyt_B5-like_heme/steroid-bd

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0016491	oxidoreductase activity
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G37280.1	CSPI03G37280.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001199	Cytochrome b5-like heme/steroid binding domain	GENE3D	G3DSA:3.10.120.10		coord: 82..179 score: 4.6
IPR001199	Cytochrome b5-like heme/steroid binding domain	PFAM	PF00173	Cyt-b5	coord: 84..178 score: 8.7
IPR001199	Cytochrome b5-like heme/steroid binding domain	SMART	SM01117	Cyt_b5_2	coord: 83..179 score: 4.1
IPR001199	Cytochrome b5-like heme/steroid binding domain	unknown	SSF55856	Cytochrome b5-like heme/steroid binding domain	coord: 81..179 score: 3.53
None	No IPR available	PANTHER	PTHR10281	MEMBRANE-ASSOCIATED PROGESTERONE RECEPTOR COMPONENT-RELATED	coord: 1..200 score: 1.0
None	No IPR available	PANTHER	PTHR10281:SF40	SUBFAMILY NOT NAMED	coord: 1..200 score: 1.0

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CSPI03G37280	Cla017468	Watermelon (97103) v1	cpiwmB198
CSPI03G37280	Csa3G815470	Cucumber (Chinese Long) v2	cpicuB120
CSPI03G37280	MELO3C009703	Melon (DHL92) v3.5.1	cpimeB214
CSPI03G37280	ClCG10G016750	Watermelon (Charleston Gray)	cpiwcgB195
CSPI03G37280	MELO3C009703.2	Melon (DHL92) v3.6.1	cpimedB208
CSPI03G37280	CsaV3_3G039610	Cucumber (Chinese Long) v3	cpicucB145
CSPI03G37280	Cla97C10G199600	Watermelon (97103) v2	cpiwmbB189
CSPI03G37280	Bhi11G002273	Wax gourd	cpiwgoB223
CSPI03G37280	Cucsa.337260	Cucumber (Gy14) v1	cgycpiB504
CSPI03G37280	CmaCh06G000590	Cucurbita maxima (Rimu)	cmacpiB839
CSPI03G37280	CmoCh06G000510	Cucurbita moschata (Rifu)	cmocpiB824
CSPI03G37280	Cp4.1LG08g13650	Cucurbita pepo (Zucchini)	cpecpiB866
CSPI03G37280	CsGy3G034760	Cucumber (Gy14) v2	cgybcpiB111
CSPI03G37280	Carg01268	Silver-seed gourd	carcpiB0178

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CSPI03G37280	Silver-seed gourd	carcpiB0280
CSPI03G37280	Cucurbita maxima (Rimu)	cmacpiB241
CSPI03G37280	Cucurbita moschata (Rifu)	cmocpiB229
CSPI03G37280	Cucurbita pepo (Zucchini)	cpecpiB599
CSPI03G37280	Cucurbita pepo (Zucchini)	cpecpiB613
CSPI03G37280	Bottle gourd (USVL1VR-Ls)	cpilsiB184