BLAST of CSPI03G31610 vs. Swiss-Prot
Match:
DET2_GOSHI (Steroid 5-alpha-reductase DET2 OS=Gossypium hirsutum GN=DET2 PE=1 SV=1)
HSP 1 Score: 358.2 bits (918), Expect = 8.4e-98
Identity = 176/262 (67.18%), Postives = 196/262 (74.81%), Query Frame = 1
Query: 33 MGWDQALFHNSLLALYLIGPPTVVSLRFLQAPYGKHIRPGWGPTMSAPLAWFLMESPTLW 92
M DQ LFH LL LY+I PT +SL FLQAPYGKH RPGWGPT+S PLAWFLMESPTLW
Sbjct: 1 MASDQTLFHYCLLTLYIIALPTWISLYFLQAPYGKHNRPGWGPTISPPLAWFLMESPTLW 60
Query: 93 LTLLLFPSGDHSSHSKALLLISPFLLHYLNRTCFYPIRLLWSGARRNAQA-DFPVLVAIM 152
LT LFPSG H + K+ LLISPFL HY NRT YP+RL AR Q FPV VA M
Sbjct: 61 LTFFLFPSGQHFYNPKSFLLISPFLFHYFNRTVLYPLRL----ARNTTQTRGFPVSVAFM 120
Query: 153 AFGFNLLNSYVQTRSVSHYV-KYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVGLKRR 212
AFGFNLLN Y+Q R VSHY YE + FWWRF G ++FV+GM VN+ +D VLVGLK++
Sbjct: 121 AFGFNLLNGYLQARWVSHYKDDYENEELFWWRFLAGLLIFVVGMWVNVRADKVLVGLKKQ 180
Query: 213 G-GGYKVPTGGWFELVSCPNYLGEIMEWLGWAVMCGSWAGLGFFLYTCANLVPRARANHT 272
G GGYK+P GG FELVSCPNY GEIMEW GWAVM SW G GFFLYTCANL+PRARA
Sbjct: 181 GDGGYKIPRGGLFELVSCPNYFGEIMEWFGWAVMTWSWVGFGFFLYTCANLMPRARATRL 240
Query: 273 WYLNKFGEVYPINRKAVIPFLY 292
WYL KF + YP +RKAVIPF+Y
Sbjct: 241 WYLEKFKDDYPKDRKAVIPFIY 258
BLAST of CSPI03G31610 vs. Swiss-Prot
Match:
DET2_ARATH (Steroid 5-alpha-reductase DET2 OS=Arabidopsis thaliana GN=DET2 PE=1 SV=2)
HSP 1 Score: 342.0 bits (876), Expect = 6.2e-93
Identity = 164/260 (63.08%), Postives = 191/260 (73.46%), Query Frame = 1
Query: 36 DQALFHNSLLALYLIGPPTVVSLRFLQAPYGKHIRPGWGPTMSAPLAWFLMESPTLWLTL 95
D+ F LL L GPPT V L+FLQAPYGKH R GWGPT+S P+AWF+MESPTLWLTL
Sbjct: 6 DKTFFRYCLLTLIFAGPPTAVLLKFLQAPYGKHNRTGWGPTVSPPIAWFVMESPTLWLTL 65
Query: 96 LLFPSGDHSSHSKALLLISPFLLHYLNRTCFYPIRLLWSG--ARRNAQADFPVLVAIMAF 155
LLFP G H+ + K+LLL SP+L+HY +RT YP+RL S A +N FP+ +A +AF
Sbjct: 66 LLFPFGRHALNPKSLLLFSPYLIHYFHRTIIYPLRLFRSSFPAGKNG---FPITIAALAF 125
Query: 156 GFNLLNSYVQTRSVSHYVK-YEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVGLKRRG- 215
FNLLN Y+Q R VSHY YE WFWWRF G VVF+ GM +NI SD LV LK+
Sbjct: 126 TFNLLNGYIQARWVSHYKDDYEDGNWFWWRFVIGMVVFITGMYINITSDRTLVRLKKENR 185
Query: 216 GGYKVPTGGWFELVSCPNYLGEIMEWLGWAVMCGSWAGLGFFLYTCANLVPRARANHTWY 275
GGY +P GGWFELVSCPNY GE +EWLGWAVM SWAG+GFFLYTC+NL PRARA+H WY
Sbjct: 186 GGYVIPRGGWFELVSCPNYFGEAIEWLGWAVMTWSWAGIGFFLYTCSNLFPRARASHKWY 245
Query: 276 LNKFGEVYPINRKAVIPFLY 292
+ KF E YP RKAVIPF+Y
Sbjct: 246 IAKFKEEYPKTRKAVIPFVY 262
BLAST of CSPI03G31610 vs. Swiss-Prot
Match:
DET2_SOLLC (Steroid 5-alpha-reductase DET2 OS=Solanum lycopersicum GN=DET2 PE=1 SV=1)
HSP 1 Score: 311.6 bits (797), Expect = 9.0e-84
Identity = 149/261 (57.09%), Postives = 187/261 (71.65%), Query Frame = 1
Query: 32 MMGWDQALFHNSLLALYLIGPPTVVSLRFLQAPYGKHIRPG-WGPTMSAPLAWFLMESPT 91
M D+ LF+ + + ++ PT + +F +PYGKH G T+S P+AW MESPT
Sbjct: 1 MFSSDENLFNFIVFFILVMAFPTFILCQFFTSPYGKHYTSADSGTTISPPIAWAFMESPT 60
Query: 92 LWLTLLLFPSGDHSSHSKALLLISPFLLHYLNRTCFYPIRLLWSGARRNAQADFPVLVAI 151
LWLT+++F G + ++ A LLISP+L HY NRT YP+RL RN + +FP+ +A+
Sbjct: 61 LWLTIIVFRLGKNYTNPLAFLLISPYLFHYTNRTIIYPLRL----RSRNTKNNFPLNIAV 120
Query: 152 MAFGFNLLNSYVQTRSVSHYVKYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVGLKRR 211
AF FNLLN+Y+Q+R VSHY Y+ D WFW RF G V+F GM +NIW+DGVL+GLK +
Sbjct: 121 TAFIFNLLNAYIQSRWVSHYANYQEDDWFWVRFGIGLVIFGSGMLLNIWADGVLLGLKSQ 180
Query: 212 GGGYKVPTGGWFELVSCPNYLGEIMEWLGWAVMCGSWAGLGFFLYTCANLVPRARANHTW 271
GGGYK+P GG F+ VS PNYLGEIMEWLGWA+M SWAGL FF+YTCANLVPRA +NH W
Sbjct: 181 GGGYKIPRGGLFDYVSSPNYLGEIMEWLGWALMTWSWAGLAFFVYTCANLVPRAVSNHKW 240
Query: 272 YLNKFGEVYPINRKAVIPFLY 292
YL KFGE YP NRKAV PFLY
Sbjct: 241 YLQKFGEDYPKNRKAVFPFLY 257
BLAST of CSPI03G31610 vs. Swiss-Prot
Match:
S5A1_RAT (3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Rattus norvegicus GN=Srd5a1 PE=1 SV=2)
HSP 1 Score: 146.4 bits (368), Expect = 5.0e-34
Identity = 89/241 (36.93%), Postives = 137/241 (56.85%), Query Frame = 1
Query: 54 TVVSLRFLQAPYGKHIRPGW-GPTMSAPLAWFLMESPTLWLTLLLF--PSGDHSSHSKAL 113
++V LR + +PYG++ P W G + A AWF+ E P++ L + P+ +
Sbjct: 27 SIVGLRSVGSPYGRY-SPQWPGIRVPARPAWFIQELPSMAWPLYEYIRPAAARLGNLPNR 86
Query: 114 LLISPFLLHYLNRTCFYPIRLLWSGARRNAQADFPVLVAIMAFGFNLLNSYVQTRSVSHY 173
+L++ FL+HY+ RT +P+ L G + F ++AF F N YVQ+R +S +
Sbjct: 87 VLLAMFLIHYVQRTLVFPV--LIRGGKPTLLVTF-----VLAFLFCTFNGYVQSRYLSQF 146
Query: 174 VKYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVGLKRRGG-GYKVPTGGWFELVSCPN 233
Y D F G ++++GM +NI SD +L L++ G GYK+P GG FE VS N
Sbjct: 147 AVYAEDWVTHPCFLTGFALWLVGMVINIHSDHILRNLRKPGETGYKIPRGGLFEYVSAAN 206
Query: 234 YLGEIMEWLGWAVMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGEVYPINRKAVIPF 291
Y GE++EW G+A+ S G+ F L+T + L+ RA+ +H WY KF E YP +RK +IPF
Sbjct: 207 YFGELVEWCGFALASWSLQGVVFALFTLSTLLTRAKQHHQWYHEKF-EDYPKSRKILIPF 258
BLAST of CSPI03G31610 vs. Swiss-Prot
Match:
S5A1_MACFA (3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Macaca fascicularis GN=SRD5A1 PE=1 SV=1)
HSP 1 Score: 145.2 bits (365), Expect = 1.1e-33
Identity = 92/245 (37.55%), Postives = 127/245 (51.84%), Query Frame = 1
Query: 50 IGPPTVVSLRFLQAPYGKHIRPGWGPTMSAPLAWFLMESPTLWLTLLLFPSGDHSSHSKA 109
+G + R YG+H P + + A AW + E P+L L L + S A
Sbjct: 27 VGCAVLARNRETNLAYGRHASPSFRVRVPARAAWVVQELPSLALPLYQYASESAPRLRSA 86
Query: 110 --LLLISPFLLHYLNRTCFYPIRLLWSGARRNAQADFPVLVAIMAFGFNLLNSYVQTRSV 169
+L++ FL+HY +R YP L G + P+L MA F N Y+Q+R +
Sbjct: 87 PNCILLAMFLVHYGHRCLIYPF--LMRGGK-----PMPLLACTMAIMFCTFNGYLQSRYL 146
Query: 170 SHYVKYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVGLKRRGG-GYKVPTGGWFELVS 229
SH+ Y D RF G +++ GM +NI SD +L L++ G GYK+P GG FE V+
Sbjct: 147 SHWAVYADDWVTDPRFLIGFGLWLAGMLINIHSDHILRNLRKPGDTGYKIPRGGLFEYVT 206
Query: 230 CPNYLGEIMEWLGWAVMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGEVYPINRKAV 289
NY GEIMEW G+A+ S G F +T L RA+ +H WYL KF E YP RK +
Sbjct: 207 AANYFGEIMEWCGYALASWSVQGAAFAFFTFCFLSGRAKEHHRWYLQKF-EEYPKFRKIL 263
Query: 290 IPFLY 292
IPFL+
Sbjct: 267 IPFLF 263
BLAST of CSPI03G31610 vs. TrEMBL
Match:
A0A0A0LAB3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G732550 PE=4 SV=1)
HSP 1 Score: 616.7 bits (1589), Expect = 1.5e-173
Identity = 288/291 (98.97%), Postives = 288/291 (98.97%), Query Frame = 1
Query: 1 MRHGTNLKTDPPQISQLDSNLQPESSNPSPTMMGWDQALFHNSLLALYLIGPPTVVSLRF 60
MRHGTNLKTDPPQISQLDSNLQPESSNPSPTMMGWDQALFHNSLLALYLIGPPTVVSLRF
Sbjct: 1 MRHGTNLKTDPPQISQLDSNLQPESSNPSPTMMGWDQALFHNSLLALYLIGPPTVVSLRF 60
Query: 61 LQAPYGKHIRPGWGPTMSAPLAWFLMESPTLWLTLLLFPSGDHSSHSKALLLISPFLLHY 120
LQAPYGKHIRPGWGPTMSAPLAWFLMESPTLWLTLLLFPSGDHSSHSKALLLISPFLLHY
Sbjct: 61 LQAPYGKHIRPGWGPTMSAPLAWFLMESPTLWLTLLLFPSGDHSSHSKALLLISPFLLHY 120
Query: 121 LNRTCFYPIRLLWSGARRNAQADFPVLVAIMAFGFNLLNSYVQTRSVSHYVKYEGDGWFW 180
LNRTCFYPIRLLWSG RRNAQ FPVLVAIMAFGFNLLNSYVQTRSVSHYVKYEGDGWFW
Sbjct: 121 LNRTCFYPIRLLWSGTRRNAQVGFPVLVAIMAFGFNLLNSYVQTRSVSHYVKYEGDGWFW 180
Query: 181 WRFFGGGVVFVMGMAVNIWSDGVLVGLKRRGGGYKVPTGGWFELVSCPNYLGEIMEWLGW 240
WRFFGGGVVFVMGMAVNIWSDGVLVGLKRRGGGYKVPTGGWFELVSCPNYLGEIMEWLGW
Sbjct: 181 WRFFGGGVVFVMGMAVNIWSDGVLVGLKRRGGGYKVPTGGWFELVSCPNYLGEIMEWLGW 240
Query: 241 AVMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGEVYPINRKAVIPFLY 292
AVMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGEVYPINRKAVIPFLY
Sbjct: 241 AVMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGEVYPINRKAVIPFLY 291
BLAST of CSPI03G31610 vs. TrEMBL
Match:
M5W8U7_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa024349mg PE=4 SV=1)
HSP 1 Score: 412.5 bits (1059), Expect = 4.2e-112
Identity = 196/259 (75.68%), Postives = 207/259 (79.92%), Query Frame = 1
Query: 33 MGWDQALFHNSLLALYLIGPPTVVSLRFLQAPYGKHIRPGWGPTMSAPLAWFLMESPTLW 92
M DQ FHN LL+LYLI PPT +SLRFLQAPYGKH RPGWGPTMS PLAWFLMESPTLW
Sbjct: 1 MASDQNFFHNCLLSLYLIAPPTYISLRFLQAPYGKHRRPGWGPTMSPPLAWFLMESPTLW 60
Query: 93 LTLLLFPSGDHSSHSKALLLISPFLLHYLNRTCFYPIRLLWSGARRNAQADFPVLVAIMA 152
LTLLLFPSG HSS+ KAL LI PFL HY +RT YP+RL + FPV VA+MA
Sbjct: 61 LTLLLFPSGLHSSNPKALFLIVPFLFHYFHRTFLYPLRLCRNTTNPKTSGGFPVSVALMA 120
Query: 153 FGFNLLNSYVQTRSVSHYVKYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVGLKRRGG 212
FGFNLLN+Y+Q R VS Y YE DGWFWWRF G VVF GMAVNIWSD VLVGLK+ G
Sbjct: 121 FGFNLLNAYLQARWVSQYKDYEADGWFWWRFCVGLVVFGYGMAVNIWSDRVLVGLKKEGK 180
Query: 213 GYKVPTGGWFELVSCPNYLGEIMEWLGWAVMCGSWAGLGFFLYTCANLVPRARANHTWYL 272
GYKVP GGWFELVSCPNY GEI+EWLGWAVM SWAGLGFFLYTCANLVPRARANH WYL
Sbjct: 181 GYKVPKGGWFELVSCPNYYGEIVEWLGWAVMTWSWAGLGFFLYTCANLVPRARANHKWYL 240
Query: 273 NKFGEVYPINRKAVIPFLY 292
KFGE YP RKAVIPFLY
Sbjct: 241 EKFGEDYPRGRKAVIPFLY 259
BLAST of CSPI03G31610 vs. TrEMBL
Match:
A0A067JY64_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_25058 PE=4 SV=1)
HSP 1 Score: 402.1 bits (1032), Expect = 5.7e-109
Identity = 183/259 (70.66%), Postives = 216/259 (83.40%), Query Frame = 1
Query: 36 DQALFHNSLLALYLIGPPTVVSLRFLQAPYGKHIRPGWGPTMSAPLAWFLMESPTLWLTL 95
DQ LF SLLA YLIGPPT +SLRFLQAPYGKH RPGWGPT+ PLAWFLMESPTLWLTL
Sbjct: 5 DQNLFDYSLLAFYLIGPPTFISLRFLQAPYGKHNRPGWGPTIFPPLAWFLMESPTLWLTL 64
Query: 96 LLFPSGDHSSHSKALLLISPFLLHYLNRTCFYPIRLLWSGARRNAQA--DFPVLVAIMAF 155
LLFP G H+++ KAL+L+SP+++HY +RTC YP+RL + +++N +A FPV VA++AF
Sbjct: 65 LLFPFGQHATNFKALILMSPYIIHYFHRTCIYPLRLYLNASQQNTKAASGFPVSVALIAF 124
Query: 156 GFNLLNSYVQTRSVSHYVK-YEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVGLKRRGG 215
GFNLLN+Y+Q+R VSHY Y+ DGWFWW+F GG VF+ GM +NIWSD VLVGLKR+GG
Sbjct: 125 GFNLLNAYLQSRWVSHYKDDYDSDGWFWWKFIGGLFVFIWGMWINIWSDRVLVGLKRQGG 184
Query: 216 GYKVPTGGWFELVSCPNYLGEIMEWLGWAVMCGSWAGLGFFLYTCANLVPRARANHTWYL 275
GYKVP GGWFELVSCPNY GEI+EW+GW++M SW G GFFLYTCANLVPRA ANH WY
Sbjct: 185 GYKVPRGGWFELVSCPNYFGEIVEWVGWSLMTWSWVGFGFFLYTCANLVPRAHANHKWYQ 244
Query: 276 NKFGEVYPINRKAVIPFLY 292
+KFGE YP +RKAVIPFLY
Sbjct: 245 DKFGEDYPKSRKAVIPFLY 263
BLAST of CSPI03G31610 vs. TrEMBL
Match:
B9IG44_POPTR (3-oxo-5-alpha-steroid 4-dehydrogenase family protein OS=Populus trichocarpa GN=POPTR_0016s11800g PE=4 SV=1)
HSP 1 Score: 394.0 bits (1011), Expect = 1.5e-106
Identity = 184/257 (71.60%), Postives = 209/257 (81.32%), Query Frame = 1
Query: 36 DQALFHNSLLALYLIGPPTVVSLRFLQAPYGKHIRPGWGPTMSAPLAWFLMESPTLWLTL 95
DQ+LFH SLL++YLIGPPT +SL+FLQAPYGKH RPGWGPT+S PLAW LMESPTLWLTL
Sbjct: 5 DQSLFHYSLLSIYLIGPPTFISLKFLQAPYGKHHRPGWGPTISPPLAWILMESPTLWLTL 64
Query: 96 LLFPSGDHSSHSKALLLISPFLLHYLNRTCFYPIRLLWSGARRNAQAD--FPVLVAIMAF 155
LLFP G H ++ KA++L+SPFLLHY +RTC YP+R+ RN++ FPV VA+MAF
Sbjct: 65 LLFPYGQHFTNPKAIILMSPFLLHYFHRTCIYPLRIY-----RNSKTTGGFPVSVAVMAF 124
Query: 156 GFNLLNSYVQTRSVSHYVKYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVGLKRRGGG 215
GFNLLN+Y+Q R VSHY G FWW+FFGG VVFV GM +N W+DGVL+GLKR GGG
Sbjct: 125 GFNLLNAYLQARWVSHYKSDYDGGLFWWKFFGGLVVFVWGMRINTWADGVLLGLKREGGG 184
Query: 216 YKVPTGGWFELVSCPNYLGEIMEWLGWAVMCGSWAGLGFFLYTCANLVPRARANHTWYLN 275
YKVP GGWFELVSCPNY GEI+EWLGWAVM SWAG GF LYTCANLVPRA ANH WYL
Sbjct: 185 YKVPRGGWFELVSCPNYFGEIVEWLGWAVMTWSWAGFGFLLYTCANLVPRACANHKWYLE 244
Query: 276 KFGEVYPINRKAVIPFL 291
KF E YP +RKAVIPFL
Sbjct: 245 KFKEDYPRSRKAVIPFL 256
BLAST of CSPI03G31610 vs. TrEMBL
Match:
A5B1R9_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_021171 PE=4 SV=1)
HSP 1 Score: 390.6 bits (1002), Expect = 1.7e-105
Identity = 183/260 (70.38%), Postives = 209/260 (80.38%), Query Frame = 1
Query: 33 MGWDQALFHNSLLALYLIGPPTVVSLRFLQAPYGKHIRPGWGPTMSAPLAWFLMESPTLW 92
M WD+ LFHNSL+ALYLI PPT +SLRFLQAPYGKH R GWGPT+ LAWFLMESPT+W
Sbjct: 1 MDWDRTLFHNSLIALYLIAPPTFISLRFLQAPYGKHHRXGWGPTVPPSLAWFLMESPTVW 60
Query: 93 LTLLLFPSGDHSSHSKALLLISPFLLHYLNRTCFYPIRLLWS-GARRNAQADFPVLVAIM 152
LTLL++P GD SS+ ++L+LIS +L HY++RT YP+ L S A+RN FPV VA+M
Sbjct: 61 LTLLIYPLGDRSSNPRSLILISIYLFHYIHRTLIYPLHLRNSTAAKRNG---FPVSVALM 120
Query: 153 AFGFNLLNSYVQTRSVSHYVKYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVGLKRRG 212
AFGFNLLN+Y+Q R VSHY YE DGWFWWRF+ G V+F+ GM VN+ SD LVGLK +G
Sbjct: 121 AFGFNLLNAYLQARWVSHYANYEDDGWFWWRFWVGLVIFLDGMLVNVRSDLALVGLKSQG 180
Query: 213 GGYKVPTGGWFELVSCPNYLGEIMEWLGWAVMCGSWAGLGFFLYTCANLVPRARANHTWY 272
GGYKVP GGWFELVSC NY GE++EWLGWAVM SW GLGF LYTCANLVPRARANH WY
Sbjct: 181 GGYKVPRGGWFELVSCANYFGEVVEWLGWAVMTWSWVGLGFLLYTCANLVPRARANHKWY 240
Query: 273 LNKFGEVYPINRKAVIPFLY 292
L KFGE YP RKAVIPFLY
Sbjct: 241 LEKFGEDYPKGRKAVIPFLY 257
BLAST of CSPI03G31610 vs. TAIR10
Match:
AT2G38050.1 (AT2G38050.1 3-oxo-5-alpha-steroid 4-dehydrogenase family protein)
HSP 1 Score: 342.0 bits (876), Expect = 3.5e-94
Identity = 164/260 (63.08%), Postives = 191/260 (73.46%), Query Frame = 1
Query: 36 DQALFHNSLLALYLIGPPTVVSLRFLQAPYGKHIRPGWGPTMSAPLAWFLMESPTLWLTL 95
D+ F LL L GPPT V L+FLQAPYGKH R GWGPT+S P+AWF+MESPTLWLTL
Sbjct: 6 DKTFFRYCLLTLIFAGPPTAVLLKFLQAPYGKHNRTGWGPTVSPPIAWFVMESPTLWLTL 65
Query: 96 LLFPSGDHSSHSKALLLISPFLLHYLNRTCFYPIRLLWSG--ARRNAQADFPVLVAIMAF 155
LLFP G H+ + K+LLL SP+L+HY +RT YP+RL S A +N FP+ +A +AF
Sbjct: 66 LLFPFGRHALNPKSLLLFSPYLIHYFHRTIIYPLRLFRSSFPAGKNG---FPITIAALAF 125
Query: 156 GFNLLNSYVQTRSVSHYVK-YEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVGLKRRG- 215
FNLLN Y+Q R VSHY YE WFWWRF G VVF+ GM +NI SD LV LK+
Sbjct: 126 TFNLLNGYIQARWVSHYKDDYEDGNWFWWRFVIGMVVFITGMYINITSDRTLVRLKKENR 185
Query: 216 GGYKVPTGGWFELVSCPNYLGEIMEWLGWAVMCGSWAGLGFFLYTCANLVPRARANHTWY 275
GGY +P GGWFELVSCPNY GE +EWLGWAVM SWAG+GFFLYTC+NL PRARA+H WY
Sbjct: 186 GGYVIPRGGWFELVSCPNYFGEAIEWLGWAVMTWSWAGIGFFLYTCSNLFPRARASHKWY 245
Query: 276 LNKFGEVYPINRKAVIPFLY 292
+ KF E YP RKAVIPF+Y
Sbjct: 246 IAKFKEEYPKTRKAVIPFVY 262
BLAST of CSPI03G31610 vs. TAIR10
Match:
AT5G16010.1 (AT5G16010.1 3-oxo-5-alpha-steroid 4-dehydrogenase family protein)
HSP 1 Score: 74.3 bits (181), Expect = 1.4e-13
Identity = 38/108 (35.19%), Postives = 67/108 (62.04%), Query Frame = 1
Query: 186 GGVVFVMGMAVNIWSDGVLVGLKRRGGG--YKVPTGGWFELVSCPNYLGEIMEWLGWAVM 245
G V+FV+G+ N++ +L L++ G YK+P GG F+++ CP+YL EI+ + + ++
Sbjct: 162 GVVMFVVGIVGNLYHHVLLAKLRKEDGKKEYKIPKGGLFDIIICPHYLFEILVFWSFFLI 221
Query: 246 CGSWAGLGFFLYTCANLVPRARANHTWYLNKFGEVYPINRKAVIPFLY 292
+ F + T L+ R+ A TWYL+KF + +P + KA+IPF++
Sbjct: 222 SQTIYSFSFAMGTMLYLIGRSYATRTWYLSKFDD-FPKHIKALIPFVF 268
BLAST of CSPI03G31610 vs. NCBI nr
Match:
gi|449439928|ref|XP_004137737.1| (PREDICTED: steroid 5-alpha-reductase DET2 [Cucumis sativus])
HSP 1 Score: 616.7 bits (1589), Expect = 2.1e-173
Identity = 288/291 (98.97%), Postives = 288/291 (98.97%), Query Frame = 1
Query: 1 MRHGTNLKTDPPQISQLDSNLQPESSNPSPTMMGWDQALFHNSLLALYLIGPPTVVSLRF 60
MRHGTNLKTDPPQISQLDSNLQPESSNPSPTMMGWDQALFHNSLLALYLIGPPTVVSLRF
Sbjct: 1 MRHGTNLKTDPPQISQLDSNLQPESSNPSPTMMGWDQALFHNSLLALYLIGPPTVVSLRF 60
Query: 61 LQAPYGKHIRPGWGPTMSAPLAWFLMESPTLWLTLLLFPSGDHSSHSKALLLISPFLLHY 120
LQAPYGKHIRPGWGPTMSAPLAWFLMESPTLWLTLLLFPSGDHSSHSKALLLISPFLLHY
Sbjct: 61 LQAPYGKHIRPGWGPTMSAPLAWFLMESPTLWLTLLLFPSGDHSSHSKALLLISPFLLHY 120
Query: 121 LNRTCFYPIRLLWSGARRNAQADFPVLVAIMAFGFNLLNSYVQTRSVSHYVKYEGDGWFW 180
LNRTCFYPIRLLWSG RRNAQ FPVLVAIMAFGFNLLNSYVQTRSVSHYVKYEGDGWFW
Sbjct: 121 LNRTCFYPIRLLWSGTRRNAQVGFPVLVAIMAFGFNLLNSYVQTRSVSHYVKYEGDGWFW 180
Query: 181 WRFFGGGVVFVMGMAVNIWSDGVLVGLKRRGGGYKVPTGGWFELVSCPNYLGEIMEWLGW 240
WRFFGGGVVFVMGMAVNIWSDGVLVGLKRRGGGYKVPTGGWFELVSCPNYLGEIMEWLGW
Sbjct: 181 WRFFGGGVVFVMGMAVNIWSDGVLVGLKRRGGGYKVPTGGWFELVSCPNYLGEIMEWLGW 240
Query: 241 AVMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGEVYPINRKAVIPFLY 292
AVMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGEVYPINRKAVIPFLY
Sbjct: 241 AVMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGEVYPINRKAVIPFLY 291
BLAST of CSPI03G31610 vs. NCBI nr
Match:
gi|659083658|ref|XP_008442471.1| (PREDICTED: steroid 5-alpha-reductase DET2 [Cucumis melo])
HSP 1 Score: 588.2 bits (1515), Expect = 8.0e-165
Identity = 276/291 (94.85%), Postives = 280/291 (96.22%), Query Frame = 1
Query: 1 MRHGTNLKTDPPQISQLDSNLQPESSNPSPTMMGWDQALFHNSLLALYLIGPPTVVSLRF 60
MR+ TNLKTD PQISQLDSNLQPESSNPSPT+MGWDQALF NSLLALYLIGPPTVVSLRF
Sbjct: 1 MRYVTNLKTDQPQISQLDSNLQPESSNPSPTVMGWDQALFRNSLLALYLIGPPTVVSLRF 60
Query: 61 LQAPYGKHIRPGWGPTMSAPLAWFLMESPTLWLTLLLFPSGDHSSHSKALLLISPFLLHY 120
LQAPYGKHIRPGWGPTMSAPLAWFLMESPTLWLTLLLFPSGDHSS SKALLLISPFLLHY
Sbjct: 61 LQAPYGKHIRPGWGPTMSAPLAWFLMESPTLWLTLLLFPSGDHSSDSKALLLISPFLLHY 120
Query: 121 LNRTCFYPIRLLWSGARRNAQADFPVLVAIMAFGFNLLNSYVQTRSVSHYVKYEGDGWFW 180
LNRTCFYPIRLLWSG RRNA A FPV+VAIMAFGFNLLN+YVQTRSVSHYVKYEGDGWFW
Sbjct: 121 LNRTCFYPIRLLWSGTRRNATAGFPVIVAIMAFGFNLLNAYVQTRSVSHYVKYEGDGWFW 180
Query: 181 WRFFGGGVVFVMGMAVNIWSDGVLVGLKRRGGGYKVPTGGWFELVSCPNYLGEIMEWLGW 240
WRFFGGGVVFVMGMAVNIWSDGVLVGLKR GGGYKVP GGWFELVSCPNYLGEIMEWLGW
Sbjct: 181 WRFFGGGVVFVMGMAVNIWSDGVLVGLKRSGGGYKVPKGGWFELVSCPNYLGEIMEWLGW 240
Query: 241 AVMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGEVYPINRKAVIPFLY 292
VMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGE YPINRKAVIPFLY
Sbjct: 241 GVMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGEDYPINRKAVIPFLY 291
BLAST of CSPI03G31610 vs. NCBI nr
Match:
gi|694389137|ref|XP_009370214.1| (PREDICTED: steroid 5-alpha-reductase DET2 [Pyrus x bretschneideri])
HSP 1 Score: 422.2 bits (1084), Expect = 7.6e-115
Identity = 200/261 (76.63%), Postives = 212/261 (81.23%), Query Frame = 1
Query: 32 MMGWDQALFHNSLLALYLIGPPTVVSLRFLQAPYGKHIRPGWGPTMSAPLAWFLMESPTL 91
M DQ FHN L+ALYLI PPT +SLRFLQAPYGKH RPGWGPTMS PLAWFLMESPTL
Sbjct: 31 MASSDQTFFHNCLIALYLIAPPTFISLRFLQAPYGKHRRPGWGPTMSPPLAWFLMESPTL 90
Query: 92 WLTLLLFPSGDHSSHSKALLLISPFLLHYLNRTCFYPIRLLWSGARRNAQAD-FPVLVAI 151
WLTLLLFPSG HSS+ KALLLI+PFL HY +RTC YP+RL + A + A FPV VA
Sbjct: 91 WLTLLLFPSGRHSSNPKALLLITPFLFHYFHRTCLYPLRLHRNTASSKSSAGGFPVSVAA 150
Query: 152 MAFGFNLLNSYVQTRSVSHYVKYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVGLKRR 211
MAFGFNLLN+Y+Q R VS Y YE DGWFWWRF GG VFV GM VNIWSD VLVGLK+
Sbjct: 151 MAFGFNLLNAYLQGRWVSEYKDYESDGWFWWRFMGGLAVFVYGMGVNIWSDSVLVGLKKE 210
Query: 212 GGGYKVPTGGWFELVSCPNYLGEIMEWLGWAVMCGSWAGLGFFLYTCANLVPRARANHTW 271
GGGYKVP GGWFELVSCPNY GEI+EW GWAVM SWAGLGFFLYTCANLVPRARANH W
Sbjct: 211 GGGYKVPKGGWFELVSCPNYYGEILEWGGWAVMTWSWAGLGFFLYTCANLVPRARANHRW 270
Query: 272 YLNKFGEVYPINRKAVIPFLY 292
YL KFGE YP RKAVIPFLY
Sbjct: 271 YLEKFGEDYPKGRKAVIPFLY 291
BLAST of CSPI03G31610 vs. NCBI nr
Match:
gi|1009158046|ref|XP_015897077.1| (PREDICTED: steroid 5-alpha-reductase DET2 [Ziziphus jujuba])
HSP 1 Score: 417.2 bits (1071), Expect = 2.4e-113
Identity = 195/259 (75.29%), Postives = 209/259 (80.69%), Query Frame = 1
Query: 33 MGWDQALFHNSLLALYLIGPPTVVSLRFLQAPYGKHIRPGWGPTMSAPLAWFLMESPTLW 92
MG D FH SLL LY IGPPT +SLRFLQAPYGKH RPGWGPTMS PLAWFLMESPT+W
Sbjct: 1 MGSDHTFFHYSLLTLYFIGPPTFISLRFLQAPYGKHQRPGWGPTMSPPLAWFLMESPTIW 60
Query: 93 LTLLLFPSGDHSSHSKALLLISPFLLHYLNRTCFYPIRLLWSGARRNAQADFPVLVAIMA 152
LTL LFP G HSS+ KAL+LISPFLLHY +RTC YP+RL + R + FPV VA++A
Sbjct: 61 LTLFLFPFGLHSSNPKALILISPFLLHYFHRTCLYPLRLHLNTKHRKTASGFPVSVALIA 120
Query: 153 FGFNLLNSYVQTRSVSHYVKYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVGLKRRGG 212
F FNLLN+Y+Q R VSHY YEGD WFWWRF GG V+F+ GMAVNIWSD VLVGLKR GG
Sbjct: 121 FVFNLLNAYLQARWVSHYRNYEGDYWFWWRFLGGLVIFLGGMAVNIWSDRVLVGLKREGG 180
Query: 213 GYKVPTGGWFELVSCPNYLGEIMEWLGWAVMCGSWAGLGFFLYTCANLVPRARANHTWYL 272
GYKVP GGWFELVSCPNY GEIMEW GWAVM SW GLGFFLYTCANLVPRA ANH WYL
Sbjct: 181 GYKVPRGGWFELVSCPNYFGEIMEWFGWAVMTWSWVGLGFFLYTCANLVPRACANHNWYL 240
Query: 273 NKFGEVYPINRKAVIPFLY 292
KF E YP RKAVIPFLY
Sbjct: 241 EKFREDYPKARKAVIPFLY 259
BLAST of CSPI03G31610 vs. NCBI nr
Match:
gi|657957127|ref|XP_008370052.1| (PREDICTED: steroid 5-alpha-reductase DET2 [Malus domestica])
HSP 1 Score: 416.8 bits (1070), Expect = 3.2e-113
Identity = 195/257 (75.88%), Postives = 208/257 (80.93%), Query Frame = 1
Query: 36 DQALFHNSLLALYLIGPPTVVSLRFLQAPYGKHIRPGWGPTMSAPLAWFLMESPTLWLTL 95
DQ FHN L+ALYLI PPT +SLRFLQAPYGKH RPGWGPTMS PLAWFLMESPTLWLTL
Sbjct: 5 DQTFFHNCLIALYLIAPPTFISLRFLQAPYGKHRRPGWGPTMSPPLAWFLMESPTLWLTL 64
Query: 96 LLFPSGDHSSHSKALLLISPFLLHYLNRTCFYPIRLLWSGARRNAQAD-FPVLVAIMAFG 155
LLFPSG HSS+ KALLLI+PFL HY +RTC YP+RL A + A FPV VA MAFG
Sbjct: 65 LLFPSGRHSSNPKALLLITPFLFHYFHRTCLYPLRLHRDTASSKSSAGGFPVSVAAMAFG 124
Query: 156 FNLLNSYVQTRSVSHYVKYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVGLKRRGGGY 215
FNLLN+Y+Q R +S Y YE DGWFWWRF GG VF GM VNIWSD VLVGLK+ GGGY
Sbjct: 125 FNLLNAYLQGRWISEYKDYESDGWFWWRFMGGLAVFGYGMGVNIWSDSVLVGLKKEGGGY 184
Query: 216 KVPTGGWFELVSCPNYLGEIMEWLGWAVMCGSWAGLGFFLYTCANLVPRARANHTWYLNK 275
KVP GGWFELVSCPNY GEI+EW GWAVM SW GLGFFLYTCANLVPRARANH WYL K
Sbjct: 185 KVPKGGWFELVSCPNYYGEIVEWAGWAVMTWSWVGLGFFLYTCANLVPRARANHRWYLEK 244
Query: 276 FGEVYPINRKAVIPFLY 292
FGE YP RKAV+PFLY
Sbjct: 245 FGEDYPKGRKAVLPFLY 261
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
DET2_GOSHI | 8.4e-98 | 67.18 | Steroid 5-alpha-reductase DET2 OS=Gossypium hirsutum GN=DET2 PE=1 SV=1 | [more] |
DET2_ARATH | 6.2e-93 | 63.08 | Steroid 5-alpha-reductase DET2 OS=Arabidopsis thaliana GN=DET2 PE=1 SV=2 | [more] |
DET2_SOLLC | 9.0e-84 | 57.09 | Steroid 5-alpha-reductase DET2 OS=Solanum lycopersicum GN=DET2 PE=1 SV=1 | [more] |
S5A1_RAT | 5.0e-34 | 36.93 | 3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Rattus norvegicus GN=Srd5a1 PE=1 SV=2 | [more] |
S5A1_MACFA | 1.1e-33 | 37.55 | 3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Macaca fascicularis GN=SRD5A1 PE=1 SV... | [more] |
Match Name | E-value | Identity | Description | |
A0A0A0LAB3_CUCSA | 1.5e-173 | 98.97 | Uncharacterized protein OS=Cucumis sativus GN=Csa_3G732550 PE=4 SV=1 | [more] |
M5W8U7_PRUPE | 4.2e-112 | 75.68 | Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa024349mg PE=4 SV=1 | [more] |
A0A067JY64_JATCU | 5.7e-109 | 70.66 | Uncharacterized protein OS=Jatropha curcas GN=JCGZ_25058 PE=4 SV=1 | [more] |
B9IG44_POPTR | 1.5e-106 | 71.60 | 3-oxo-5-alpha-steroid 4-dehydrogenase family protein OS=Populus trichocarpa GN=P... | [more] |
A5B1R9_VITVI | 1.7e-105 | 70.38 | Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_021171 PE=4 SV=1 | [more] |
Match Name | E-value | Identity | Description | |
AT2G38050.1 | 3.5e-94 | 63.08 | 3-oxo-5-alpha-steroid 4-dehydrogenase family protein | [more] |
AT5G16010.1 | 1.4e-13 | 35.19 | 3-oxo-5-alpha-steroid 4-dehydrogenase family protein | [more] |