CSPI03G30280 (gene) Wild cucumber (PI 183967) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CSPI03G30280
Type	gene
Organism	Cucumis sativus (Wild cucumber (PI 183967))
Description	Chaperone protein dnaJ 8
Location	Chr3 : 27678224 .. 27680167 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TAAATAATAGAAAAGAAGATAAGAAGAGAATAAAAGTCTGATGGAAGGAACACGTCATCTCTCTCTCTCTCTCTAAAATTGACCCATTGGATAAGAACCGACGATCTCGCCCCTCCACACCCTCCCTATTCCCAATCTTCTATTTATTTCCCTTCCCCTTCCCTTTCCTTTTCCTCCATTCTTCATTCCAACGTTACTACACTTTCCTCTTCCTTTCAACACCCTCCCATTTCCAAAATTAATGGCCTCCGTCGCTACTGCTGGATTCATCGCACCATCTTCCTCTTCCTCTTCATGTTTTTCCCATATTAATCGTCCAAACAAGAATCCGACTTCCACTAATCAATTTAGAGTTTCTTGTTCTTATTCTTCTTCTTCCGTCGCCGATCCTTATCGAACTCTTCGGATTCAACCCGGTTCCTCTGAATCTGAAGTCAAAAAAGCCTTCCGACGTCTCGCTCTTAAGGTTTCTTCTTTTCTTTCTTTCCATTTCTTTATATTACAGGATTTGTTCCATCCTACTGATTCTTACGATTTCTTTGATTCTTTTTTTTATCAGTATCATCCTGATGTTTGTAAAGGGAGAAATTGTGGCGTTCAGTTTGAACAAATCAACGAAGCTTACGTTGTACGTGATCATACCCTCTTTTTTCTTTTTAATTATTTACTCAAATTTGAATCAATTATTGAAATGATTGATTTTTTTTTTCTTTCTTTCTTATAATAATAATAATTGTGCACCTATTATTGCAATAACTAATTTTTGTTTCTTTCCTCACATAATTACTAAACTAATTAAATATATAGACATTTTAGAGGAAATAGTTTGAATTTCACTATATGTATCGGAATTGAGTGTTTGATTAGGTTCATGTCGGTTTCATAGTGTTTTCGGTCATTCTATCATTGATTTTCCTATACCTATTTGTCGTATATCATATCAGGTTAAATTATATACATACACACACTTGCACTTTGTCTTTTGCTTTTATTGGGTCCCAGATTGACCCAAATTTGTTTAAGCCTTTTTATGTAAATTGCAAATTTGATCGTAGCGCTGTGTTGGTTATCATAGATTGGGTAGAGATTAAGTTGGGTCCCAAACACTTCCACCGTGTTTGTCTTATACATAATATCTACTATTGGCCTATTTATAAATTCTAATAATCGATCAAATTTGTATGTGAATTGAATTCTTGCAACAGATCTGTGTTATGAAGATAGTTTGAATTTTGCTAACGAGGTGATTTTTGATTTGGTGAAACAGATTGTGATGAATAACTTAAGAGGTATAGCAACACCAATAGAAGAGACATATGAAACAAAATATTATGAAGGGACTGATGAACCGACGAGAAAATATGGGGAACCAGATTGGGACTCGTATGAAGAATGGATGGGATATGAAGGAGCGTGGATGGGTGATTATTCATCTCAATATTAATCCTGAAATTTAAAACCCAAAAACAAAAAATTAAAAGTCAGTTATGATTTGCACATAGAATTGGTCTTTAATCTATCAACATCATCAACAACCAACGGTTGTGATGATTTGGTTTGGCTTTGTATATATGGTGGAAAAAGAGGGGAAGTTGAAAATTGGTATTAATTCACTAATTTGTGTACCCCTTTATTTTAGTGGTGGTGGTGTCGTTGTTGTACATATGGTCAAGAACAGAGCCTTTTGGTCTTTTAATAATATGAAAAGGCTTTGAAGGAATGGCCTATCAAAGTTGTGGTGGGGCATTTGTGTTTTTATCTTTTTTTAAACTCTTGTCTGGTTGTTTGCCCAATAGAGGAGGTGGTGCTTTTTTGTGTACTCGTTTCTGTCCAATATTATCCGTTTGTGAATAAAATTAAAAAGGTGGGGGGTAGGAATATAACTATTACCTCTGTTGAGTTTCATTTGAATGGAAAGGAATCCCCTTCGGGAATATTCTGGAA

mRNA sequence

ATGGCCTCCGTCGCTACTGCTGGATTCATCGCACCATCTTCCTCTTCCTCTTCATGTTTTTCCCATATTAATCGTCCAAACAAGAATCCGACTTCCACTAATCAATTTAGAGTTTCTTGTTCTTATTCTTCTTCTTCCGTCGCCGATCCTTATCGAACTCTTCGGATTCAACCCGGTTCCTCTGAATCTGAAGTCAAAAAAGCCTTCCGACGTCTCGCTCTTAAGTATCATCCTGATGTTTGTAAAGGGAGAAATTGTGGCGTTCAGTTTGAACAAATCAACGAAGCTTACGTTATTGTGATGAATAACTTAAGAGGTATAGCAACACCAATAGAAGAGACATATGAAACAAAATATTATGAAGGGACTGATGAACCGACGAGAAAATATGGGGAACCAGATTGGGACTCGTATGAAGAATGGATGGGATATGAAGGAGCGTGGATGGGTGATTATTCATCTCAATATTAA

Coding sequence (CDS)

ATGGCCTCCGTCGCTACTGCTGGATTCATCGCACCATCTTCCTCTTCCTCTTCATGTTTTTCCCATATTAATCGTCCAAACAAGAATCCGACTTCCACTAATCAATTTAGAGTTTCTTGTTCTTATTCTTCTTCTTCCGTCGCCGATCCTTATCGAACTCTTCGGATTCAACCCGGTTCCTCTGAATCTGAAGTCAAAAAAGCCTTCCGACGTCTCGCTCTTAAGTATCATCCTGATGTTTGTAAAGGGAGAAATTGTGGCGTTCAGTTTGAACAAATCAACGAAGCTTACGTTATTGTGATGAATAACTTAAGAGGTATAGCAACACCAATAGAAGAGACATATGAAACAAAATATTATGAAGGGACTGATGAACCGACGAGAAAATATGGGGAACCAGATTGGGACTCGTATGAAGAATGGATGGGATATGAAGGAGCGTGGATGGGTGATTATTCATCTCAATATTAA

Homology

[Close]

BLAST of CSPI03G30280 vs. Swiss-Prot
Match: DNAJ8_ARATH (Chaperone protein dnaJ 8, chloroplastic OS=Arabidopsis thaliana GN=ATJ8 PE=2 SV=1)

HSP 1 Score: 138.3 bits (347), Expect = 7.4e-32
Identity = 80/141 (56.74%), Postives = 97/141 (68.79%), Query Frame = 1


Query: 14  SSSSSCFSHINRPNKNPTSTNQFRVSCSYSSSSVADPYRTLRIQPGSSESEVKKAFRRLA 73
           SSSSS F   N   KN    N+ +V CS SSSSV DPY+TL+I+P SSE EVKKAFR+LA
Sbjct: 21  SSSSSSFRLKNSRRKNTKMLNRSKVVCS-SSSSVMDPYKTLKIRPDSSEYEVKKAFRQLA 80

Query: 74  LKYHPDVCKGRNCGVQFEQINEAYVIVMNNLRGIATPIEETYETKYYEGTDEPTRKYGEP 133
            KYHPDVC+G NCGVQF+ INEAY IV   L+ I   +E T E + ++  DE      +P
Sbjct: 81  KKYHPDVCRGSNCGVQFQTINEAYDIV---LKQIKNQMEGTEEFEPFDVYDEGLNGMNDP 140

Query: 134 DWDSYEEWMGYEGAWMGDYSS 155
           D D++EEWMG+EGA   DYSS
Sbjct: 141 DCDTWEEWMGWEGAGTRDYSS 157

[Close]

BLAST of CSPI03G30280 vs. Swiss-Prot
Match: DNAJ2_AQUAE (Chaperone protein DnaJ 2 OS=Aquifex aeolicus (strain VF5) GN=dnaJ2 PE=3 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 3.9e-09
Identity = 27/58 (46.55%), Postives = 40/58 (68.97%), Query Frame = 1


Query: 43  SSSSVADPYRTLRIQPGSSESEVKKAFRRLALKYHPDVCKGRNCGVQFEQINEAYVIV 101
           +SS+  D Y  L +   +S+ E+KKA+RRL  KYHPD+CK   C  +F++INEAY ++
Sbjct: 2   ASSTKKDYYEILGVPRNASQEEIKKAYRRLVRKYHPDICKKPECEEKFKEINEAYQVL 59

[Close]

BLAST of CSPI03G30280 vs. Swiss-Prot
Match: CBPA_PSEP1 (Curved DNA-binding protein OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=cbpA PE=3 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 5.7e-08
Identity = 38/107 (35.51%), Postives = 57/107 (53.27%), Query Frame = 1


Query: 49  DPYRTLRIQPGSSESEVKKAFRRLALKYHPDVCKGRNCGVQFEQINEAYVIVMNNLRGIA 108
           D Y+ L ++P + E  +K A+R+LA KYHPDV K R+   +F++ NEAY ++     G A
Sbjct: 5   DYYKILGVEPTADEKAIKAAYRKLARKYHPDVSKERDAEEKFKEANEAYEVL-----GDA 64

Query: 109 TPIEETYETKYYEGTDEPTRKY-GEPDWDSYEEWMGYEGAWMGDYSS 155
               E  E + Y G  +  R +   P W+S     G+EG    D+ S
Sbjct: 65  QKRAEFDEIRKYGG--QHGRPFQAPPGWESRGGGGGFEGGDFSDFFS 104

[Close]

BLAST of CSPI03G30280 vs. Swiss-Prot
Match: CBPA_PSEPK (Curved DNA-binding protein OS=Pseudomonas putida (strain KT2440) GN=cbpA PE=3 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 5.7e-08
Identity = 38/107 (35.51%), Postives = 57/107 (53.27%), Query Frame = 1


Query: 49  DPYRTLRIQPGSSESEVKKAFRRLALKYHPDVCKGRNCGVQFEQINEAYVIVMNNLRGIA 108
           D Y+ L ++P + E  +K A+R+LA KYHPDV K R+   +F++ NEAY ++     G A
Sbjct: 5   DYYKILGVEPTADEKAIKAAYRKLARKYHPDVSKERDAEEKFKEANEAYEVL-----GDA 64

Query: 109 TPIEETYETKYYEGTDEPTRKY-GEPDWDSYEEWMGYEGAWMGDYSS 155
               E  E + Y G  +  R +   P W+S     G+EG    D+ S
Sbjct: 65  QKRAEFDEIRKYGG--QHGRPFQAPPGWESRGGGGGFEGGDFSDFFS 104

[Close]

BLAST of CSPI03G30280 vs. Swiss-Prot
Match: DNAJ_MANSM (Chaperone protein DnaJ OS=Mannheimia succiniciproducens (strain MBEL55E) GN=dnaJ PE=3 SV=2)

HSP 1 Score: 58.5 bits (140), Expect = 7.4e-08
Identity = 26/55 (47.27%), Postives = 39/55 (70.91%), Query Frame = 1


Query: 49  DPYRTLRIQPGSSESEVKKAFRRLALKYHPDVCKGRNCGVQ-FEQINEAYVIVMN 103
           D Y TL +Q G+ E E+K+A++RLA+KYHPD   G     + F+++NEAY I+M+
Sbjct: 5   DYYETLGVQKGADEKEIKRAYKRLAMKYHPDRTNGDKAAEEKFKEVNEAYEILMD 59

[Close]

BLAST of CSPI03G30280 vs. TrEMBL
Match: A0A0A0LD63_CUCSA (Chaperone protein dnaJ 8 OS=Cucumis sativus GN=Csa_3G710790 PE=4 SV=1)

HSP 1 Score: 316.2 bits (809), Expect = 2.2e-83
Identity = 154/156 (98.72%), Postives = 155/156 (99.36%), Query Frame = 1


Query: 1   MASVATAGFIAPSSSSSSCFSHINRPNKNPTSTNQFRVSCSYSSSSVADPYRTLRIQPGS 60
           MASVATAGFIAPSSSSSSCFSH NRPNKNPTSTNQFRVSCSYSSSS+ADPYRTLRIQPGS
Sbjct: 1   MASVATAGFIAPSSSSSSCFSHTNRPNKNPTSTNQFRVSCSYSSSSLADPYRTLRIQPGS 60

Query: 61  SESEVKKAFRRLALKYHPDVCKGRNCGVQFEQINEAYVIVMNNLRGIATPIEETYETKYY 120
           SESEVKKAFRRLALKYHPDVCKGRNCGVQFEQINEAYVIVMNNLRGIATPIEETYETKYY
Sbjct: 61  SESEVKKAFRRLALKYHPDVCKGRNCGVQFEQINEAYVIVMNNLRGIATPIEETYETKYY 120

Query: 121 EGTDEPTRKYGEPDWDSYEEWMGYEGAWMGDYSSQY 157
           EGTDEPTRKYGEPDWDSYEEWMGYEGAWMGDYSSQY
Sbjct: 121 EGTDEPTRKYGEPDWDSYEEWMGYEGAWMGDYSSQY 156

[Close]

BLAST of CSPI03G30280 vs. TrEMBL
Match: A0A0A0LF08_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G710780 PE=4 SV=1)

HSP 1 Score: 278.1 bits (710), Expect = 6.6e-72
Identity = 145/158 (91.77%), Postives = 146/158 (92.41%), Query Frame = 1


Query: 1   MASVATAGFIAPSSSSSS--CFSHINRPNKNPTSTNQFRVSCSYSSSSVADPYRTLRIQP 60
           MASVATAGFIAPSSSSSS  CF H NRPNKN TSTNQFRVS S+SSS VADPYRTLRIQP
Sbjct: 1   MASVATAGFIAPSSSSSSSSCFPHTNRPNKNRTSTNQFRVSSSFSSS-VADPYRTLRIQP 60

Query: 61  GSSESEVKKAFRRLALKYHPDVCKGRNCGVQFEQINEAYVIVMNNLRGIATPIEETYETK 120
           GSSESEVKKAFRRLALKYHPDVCKGRNCGVQFEQINEAYVIVMNNLRGIAT I ETYETK
Sbjct: 61  GSSESEVKKAFRRLALKYHPDVCKGRNCGVQFEQINEAYVIVMNNLRGIATSI-ETYETK 120

Query: 121 YYEGTDEPTRKYGEPDWDSYEEWMGYEGAWMGDYSSQY 157
           Y EGTDEP  KYGEPDWDSYEEWMGYEGAWMGDYSSQY
Sbjct: 121 YSEGTDEPKTKYGEPDWDSYEEWMGYEGAWMGDYSSQY 156

[Close]

BLAST of CSPI03G30280 vs. TrEMBL
Match: M5WS61_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa012860mg PE=4 SV=1)

HSP 1 Score: 167.9 bits (424), Expect = 9.6e-39
Identity = 96/154 (62.34%), Postives = 115/154 (74.68%), Query Frame = 1


Query: 1   MASVATAGFIAPSSSSSSCFSHINRPNKNPTSTNQFRVSCSYSSSSVADPYRTLRIQPGS 60
           MA+VA AG I  + SS + F   ++P K  T  N+  VSC YSS  V DPY+TL+IQPG+
Sbjct: 1   MAAVA-AGVIGGNGSSWNLFK--DKPRKKKT-VNKVMVSCVYSS--VMDPYQTLKIQPGA 60

Query: 61  SESEVKKAFRRLALKYHPDVCKGRNCGVQFEQINEAYVIVMNNLRGIATPIEETYETKYY 120
           SESEV+KAFR+LALKYHPDVC+G NCGVQF QINEAY IVM+NLRG +TP    YET Y 
Sbjct: 61  SESEVRKAFRQLALKYHPDVCRGNNCGVQFHQINEAYDIVMSNLRGESTP-SPMYET-YD 120

Query: 121 EGTDEPTRKYGEPDWDSYEEWMGYEGAWMGDYSS 155
           EG DEP R   +PDWD +EEWMG+EGA + DY+S
Sbjct: 121 EGVDEPMRGMNDPDWDMWEEWMGWEGAGIRDYTS 146

[Close]

BLAST of CSPI03G30280 vs. TrEMBL
Match: A0A0B2P4K3_GLYSO (Chaperone protein dnaJ 8, chloroplastic OS=Glycine soja GN=glysoja_032687 PE=4 SV=1)

HSP 1 Score: 166.0 bits (419), Expect = 3.7e-38
Identity = 90/152 (59.21%), Postives = 110/152 (72.37%), Query Frame = 1


Query: 3   SVATAGFIAPSSSSSSCFSHINRPNKNPTSTNQFRVSCSYSSSSVADPYRTLRIQPGSSE 62
           + ATAG +  + SS+S      +  K  T  N+ R+SCSYSSS V DPY+TLRIQ G+SE
Sbjct: 2   AAATAGVVGGNGSSASWMRFKGKERKQ-TKMNKSRISCSYSSS-VMDPYKTLRIQRGASE 61

Query: 63  SEVKKAFRRLALKYHPDVCKGRNCGVQFEQINEAYVIVMNNLRGIATPIEETYETKYYEG 122
           SEV+KAFR+LAL+YHPDVC+G NCGVQF +INEAY IVM NLRG  +   E+YE  Y  G
Sbjct: 62  SEVRKAFRQLALQYHPDVCRGSNCGVQFHEINEAYDIVMANLRG-ESNATESYEAYYDAG 121

Query: 123 TDEPTRKYGEPDWDSYEEWMGYEGAWMGDYSS 155
            DEP R   +PDWD +EEWMG+EGA + DYSS
Sbjct: 122 IDEPLRGMNDPDWDMWEEWMGWEGAGIRDYSS 150

[Close]

BLAST of CSPI03G30280 vs. TrEMBL
Match: A0A151RB82_CAJCA (Uncharacterized protein OS=Cajanus cajan GN=KK1_039066 PE=4 SV=1)

HSP 1 Score: 164.1 bits (414), Expect = 1.4e-37
Identity = 91/156 (58.33%), Postives = 113/156 (72.44%), Query Frame = 1


Query: 2   ASVATAGFIAPSSSSSSCFSHINRPNKNPTSTNQFRVSCSY--SSSSVADPYRTLRIQPG 61
           A+ AT+G +A + SS+S      R  KN    N+ RV CSY  SSSSV DPY+TLRIQ G
Sbjct: 4   AAAATSGVVAGNGSSASWMKFKRRERKN----NKSRVCCSYYSSSSSVMDPYKTLRIQKG 63

Query: 62  SSESEVKKAFRRLALKYHPDVCKGRNCGVQFEQINEAYVIVMNNLRGIATPIEETYETKY 121
           +SESEV+KAFR+LAL+YHPDVC+G NCGVQF +INEAY IVM NLRG +    ++YE  Y
Sbjct: 64  ASESEVRKAFRQLALQYHPDVCRGNNCGVQFHEINEAYDIVMANLRGESNE-AQSYEAYY 123

Query: 122 YE-GTDEPTRKYGEPDWDSYEEWMGYEGAWMGDYSS 155
            + G DEP R   +PDWD +EEWMG+EGA + DY+S
Sbjct: 124 DDGGIDEPLRGMNDPDWDMWEEWMGWEGAGIRDYTS 154

[Close]

BLAST of CSPI03G30280 vs. TAIR10
Match: AT1G80920.1 (AT1G80920.1 Chaperone DnaJ-domain superfamily protein)

HSP 1 Score: 138.3 bits (347), Expect = 4.1e-33
Identity = 80/141 (56.74%), Postives = 97/141 (68.79%), Query Frame = 1


Query: 14  SSSSSCFSHINRPNKNPTSTNQFRVSCSYSSSSVADPYRTLRIQPGSSESEVKKAFRRLA 73
           SSSSS F   N   KN    N+ +V CS SSSSV DPY+TL+I+P SSE EVKKAFR+LA
Sbjct: 21  SSSSSSFRLKNSRRKNTKMLNRSKVVCS-SSSSVMDPYKTLKIRPDSSEYEVKKAFRQLA 80

Query: 74  LKYHPDVCKGRNCGVQFEQINEAYVIVMNNLRGIATPIEETYETKYYEGTDEPTRKYGEP 133
            KYHPDVC+G NCGVQF+ INEAY IV   L+ I   +E T E + ++  DE      +P
Sbjct: 81  KKYHPDVCRGSNCGVQFQTINEAYDIV---LKQIKNQMEGTEEFEPFDVYDEGLNGMNDP 140

Query: 134 DWDSYEEWMGYEGAWMGDYSS 155
           D D++EEWMG+EGA   DYSS
Sbjct: 141 DCDTWEEWMGWEGAGTRDYSS 157

[Close]

BLAST of CSPI03G30280 vs. TAIR10
Match: AT1G72070.1 (AT1G72070.1 Chaperone DnaJ-domain superfamily protein)

HSP 1 Score: 60.8 bits (146), Expect = 8.4e-10
Identity = 42/112 (37.50%), Postives = 58/112 (51.79%), Query Frame = 1


Query: 35  QFRVSCSYSSSSVADPYRTLRIQPGSSESEVKKAFRRLALKYHPDVCKGRNCGVQFEQIN 94
           QF   C  S       Y  L + P +S++EVK+AF+RLALKYHPDV KG++    F++I 
Sbjct: 27  QFPTRCCLSPD--LSHYTVLGLTPLASQTEVKRAFKRLALKYHPDVHKGQD--KDFKEIK 86

Query: 95  EAYVIVMNNLRGIATPIEETYETKYYEGTDEPTRKYGEPDWDSYEEWMGYEG 147
            AY  +M   +     +E T              + GE   D +EEWMG+EG
Sbjct: 87  SAYECLMQKFKKEEEEMEIT--------------EMGE--IDEWEEWMGFEG 118

[Close]

BLAST of CSPI03G30280 vs. TAIR10
Match: AT5G59610.1 (AT5G59610.1 Chaperone DnaJ-domain superfamily protein)

HSP 1 Score: 54.3 bits (129), Expect = 7.9e-08
Identity = 26/72 (36.11%), Postives = 45/72 (62.50%), Query Frame = 1


Query: 32  STNQFRVSCSYSSSSVADPYRTLRIQPGSSESEVKKAFRRLALKYHPDVCKGRNCGVQFE 91
           S+ Q R +  + + + + PY  L + P ++  ++K+A+R+LALKYHPDV K  N   +F 
Sbjct: 56  SSIQRRRNRIFVTRARSSPYEILGVSPSATPQDIKRAYRKLALKYHPDVNKEANAQEKFL 115

Query: 92  QINEAYVIVMNN 104
           +I  AY  ++N+
Sbjct: 116 KIKHAYTTLINS 127

[Close]

BLAST of CSPI03G30280 vs. TAIR10
Match: AT3G08970.1 (AT3G08970.1 DNAJ heat shock N-terminal domain-containing protein)

HSP 1 Score: 50.4 bits (119), Expect = 1.1e-06
Identity = 21/52 (40.38%), Postives = 33/52 (63.46%), Query Frame = 1


Query: 49  DPYRTLRIQPGSSESEVKKAFRRLALKYHPDVCKGRNCGVQFEQINEAYVIV 101
           DPY+ L +   + + E++KAF + +LKYHPD  K +    +F +IN AY I+
Sbjct: 27  DPYKVLGVSKDAKQREIQKAFHKQSLKYHPDKNKDKGAQEKFAEINNAYEIL 78

[Close]

BLAST of CSPI03G30280 vs. TAIR10
Match: AT4G21180.1 (AT4G21180.1 DnaJ / Sec63 Brl domains-containing protein)

HSP 1 Score: 49.7 bits (117), Expect = 1.9e-06
Identity = 30/91 (32.97%), Postives = 46/91 (50.55%), Query Frame = 1


Query: 45  SSVADPYRTLRIQPGSSESEVKKAFRRLALKYHPDVCKGRNCGVQF-EQINEAYVIVMNN 104
           S + +P+  L ++PG+S+SE+KKA+RRL+++YHPD          F E I +AY      
Sbjct: 95  SQLFEPFGILGLEPGASDSEIKKAYRRLSIQYHPDKNPDPEANKYFVESIAKAY------ 154

Query: 105 LRGIATPIEETYETKYYEGTDEPTRKYGEPD 135
            + +  P+             E   KYG PD
Sbjct: 155 -QALTDPLSR-----------ENFEKYGHPD 167

[Close]

BLAST of CSPI03G30280 vs. NCBI nr
Match: gi|778682940|ref|XP_004137653.2| (PREDICTED: chaperone protein dnaJ 8, chloroplastic-like [Cucumis sativus])

HSP 1 Score: 316.2 bits (809), Expect = 3.1e-83
Identity = 154/156 (98.72%), Postives = 155/156 (99.36%), Query Frame = 1


Query: 1   MASVATAGFIAPSSSSSSCFSHINRPNKNPTSTNQFRVSCSYSSSSVADPYRTLRIQPGS 60
           MASVATAGFIAPSSSSSSCFSH NRPNKNPTSTNQFRVSCSYSSSS+ADPYRTLRIQPGS
Sbjct: 1   MASVATAGFIAPSSSSSSCFSHTNRPNKNPTSTNQFRVSCSYSSSSLADPYRTLRIQPGS 60

Query: 61  SESEVKKAFRRLALKYHPDVCKGRNCGVQFEQINEAYVIVMNNLRGIATPIEETYETKYY 120
           SESEVKKAFRRLALKYHPDVCKGRNCGVQFEQINEAYVIVMNNLRGIATPIEETYETKYY
Sbjct: 61  SESEVKKAFRRLALKYHPDVCKGRNCGVQFEQINEAYVIVMNNLRGIATPIEETYETKYY 120

Query: 121 EGTDEPTRKYGEPDWDSYEEWMGYEGAWMGDYSSQY 157
           EGTDEPTRKYGEPDWDSYEEWMGYEGAWMGDYSSQY
Sbjct: 121 EGTDEPTRKYGEPDWDSYEEWMGYEGAWMGDYSSQY 156

[Close]

BLAST of CSPI03G30280 vs. NCBI nr
Match: gi|778682937|ref|XP_011651813.1| (PREDICTED: chaperone protein dnaJ 8, chloroplastic-like [Cucumis sativus])

HSP 1 Score: 278.1 bits (710), Expect = 9.5e-72
Identity = 145/158 (91.77%), Postives = 146/158 (92.41%), Query Frame = 1


Query: 1   MASVATAGFIAPSSSSSS--CFSHINRPNKNPTSTNQFRVSCSYSSSSVADPYRTLRIQP 60
           MASVATAGFIAPSSSSSS  CF H NRPNKN TSTNQFRVS S+SSS VADPYRTLRIQP
Sbjct: 1   MASVATAGFIAPSSSSSSSSCFPHTNRPNKNRTSTNQFRVSSSFSSS-VADPYRTLRIQP 60

Query: 61  GSSESEVKKAFRRLALKYHPDVCKGRNCGVQFEQINEAYVIVMNNLRGIATPIEETYETK 120
           GSSESEVKKAFRRLALKYHPDVCKGRNCGVQFEQINEAYVIVMNNLRGIAT I ETYETK
Sbjct: 61  GSSESEVKKAFRRLALKYHPDVCKGRNCGVQFEQINEAYVIVMNNLRGIATSI-ETYETK 120

Query: 121 YYEGTDEPTRKYGEPDWDSYEEWMGYEGAWMGDYSSQY 157
           Y EGTDEP  KYGEPDWDSYEEWMGYEGAWMGDYSSQY
Sbjct: 121 YSEGTDEPKTKYGEPDWDSYEEWMGYEGAWMGDYSSQY 156

[Close]

BLAST of CSPI03G30280 vs. NCBI nr
Match: gi|659130374|ref|XP_008465135.1| (PREDICTED: chaperone protein dnaJ 8, chloroplastic [Cucumis melo])

HSP 1 Score: 226.1 bits (575), Expect = 4.3e-56
Identity = 122/157 (77.71%), Postives = 132/157 (84.08%), Query Frame = 1


Query: 1   MASVATAGFIA---PSSSSSSCFSHINRPNKNPTSTNQFRVSCSYSSSSVADPYRTLRIQ 60
           MASVATAGFIA    SSSSSSC  + NRP KN TS+NQ RVSCS SSSSVADPYRTLRIQ
Sbjct: 1   MASVATAGFIARSSSSSSSSSCIRNTNRPKKNRTSSNQLRVSCS-SSSSVADPYRTLRIQ 60

Query: 61  PGSSESEVKKAFRRLALKYHPDVCKGRNCGVQFEQINEAYVIVMNNLRGIATPIEETYET 120
           PG+SESEVKKAFR LAL+YHPDVC+G NCGVQF QINEAY IVMNNLRGI+TPI ETYET
Sbjct: 61  PGASESEVKKAFRHLALQYHPDVCRGSNCGVQFHQINEAYDIVMNNLRGISTPI-ETYET 120

Query: 121 KYYEGTDEPTRKYGEPDWDSYEEWMGYEGAWMGDYSS 155
            Y EG DEP R  G+PDWD +EEWMG+EGA + DYSS
Sbjct: 121 IYDEGIDEPMRGMGDPDWDLWEEWMGWEGAGIRDYSS 155

[Close]

BLAST of CSPI03G30280 vs. NCBI nr
Match: gi|595923676|ref|XP_007214842.1| (hypothetical protein PRUPE_ppa012860mg [Prunus persica])

HSP 1 Score: 167.9 bits (424), Expect = 1.4e-38
Identity = 96/154 (62.34%), Postives = 115/154 (74.68%), Query Frame = 1


Query: 1   MASVATAGFIAPSSSSSSCFSHINRPNKNPTSTNQFRVSCSYSSSSVADPYRTLRIQPGS 60
           MA+VA AG I  + SS + F   ++P K  T  N+  VSC YSS  V DPY+TL+IQPG+
Sbjct: 1   MAAVA-AGVIGGNGSSWNLFK--DKPRKKKT-VNKVMVSCVYSS--VMDPYQTLKIQPGA 60

Query: 61  SESEVKKAFRRLALKYHPDVCKGRNCGVQFEQINEAYVIVMNNLRGIATPIEETYETKYY 120
           SESEV+KAFR+LALKYHPDVC+G NCGVQF QINEAY IVM+NLRG +TP    YET Y 
Sbjct: 61  SESEVRKAFRQLALKYHPDVCRGNNCGVQFHQINEAYDIVMSNLRGESTP-SPMYET-YD 120

Query: 121 EGTDEPTRKYGEPDWDSYEEWMGYEGAWMGDYSS 155
           EG DEP R   +PDWD +EEWMG+EGA + DY+S
Sbjct: 121 EGVDEPMRGMNDPDWDMWEEWMGWEGAGIRDYTS 146

[Close]

BLAST of CSPI03G30280 vs. NCBI nr
Match: gi|1021570934|ref|XP_016176636.1| (PREDICTED: chaperone protein dnaJ 8, chloroplastic-like [Arachis ipaensis])

HSP 1 Score: 166.8 bits (421), Expect = 3.1e-38
Identity = 95/156 (60.90%), Postives = 112/156 (71.79%), Query Frame = 1


Query: 1   MASVATAGFIAPSSSSSSCFSHINRPNKNPTSTNQFRVSCSYSSSSVADPYRTLRIQPGS 60
           MA+ + AG +    SSSS +    + NK     N+FR+SC  SSSSVADPY+TLRIQP +
Sbjct: 1   MAAASVAGVVGGKGSSSSSWMKQFKGNKGK-KMNKFRISC--SSSSVADPYKTLRIQPDA 60

Query: 61  SESEVKKAFRRLALKYHPDVCKGRNCGVQFEQINEAYVIVMNNLRGIATPIEETYETKYY 120
           SESEV+KAFR+LALKYHPDVC+G NCGVQF QINEAY IVM NLRG +    E Y   YY
Sbjct: 61  SESEVRKAFRQLALKYHPDVCRGNNCGVQFHQINEAYDIVMANLRGESNE-TEVYNV-YY 120

Query: 121 E--GTDEPTRKYGEPDWDSYEEWMGYEGAWMGDYSS 155
           E  G DEP R   +PDWD +EEWMG+EGA + DYSS
Sbjct: 121 EDKGIDEPLRGMNDPDWDMWEEWMGWEGAGIRDYSS 151

The following BLAST results are available for this feature:

BLAST of CSPI03G30280 vs. Swiss-Prot


Match Name	E-value	Identity	Description
DNAJ8_ARATH	7.4e-32	56.74	Chaperone protein dnaJ 8, chloroplastic OS=Arabidopsis thaliana GN=ATJ8 PE=2 SV=...	[more]
DNAJ2_AQUAE	3.9e-09	46.55	Chaperone protein DnaJ 2 OS=Aquifex aeolicus (strain VF5) GN=dnaJ2 PE=3 SV=1	[more]
CBPA_PSEP1	5.7e-08	35.51	Curved DNA-binding protein OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=cb...	[more]
CBPA_PSEPK	5.7e-08	35.51	Curved DNA-binding protein OS=Pseudomonas putida (strain KT2440) GN=cbpA PE=3 SV...	[more]
DNAJ_MANSM	7.4e-08	47.27	Chaperone protein DnaJ OS=Mannheimia succiniciproducens (strain MBEL55E) GN=dnaJ...	[more]

BLAST of CSPI03G30280 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LD63_CUCSA	2.2e-83	98.72	Chaperone protein dnaJ 8 OS=Cucumis sativus GN=Csa_3G710790 PE=4 SV=1	[more]
A0A0A0LF08_CUCSA	6.6e-72	91.77	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G710780 PE=4 SV=1	[more]
M5WS61_PRUPE	9.6e-39	62.34	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa012860mg PE=4 SV=1	[more]
A0A0B2P4K3_GLYSO	3.7e-38	59.21	Chaperone protein dnaJ 8, chloroplastic OS=Glycine soja GN=glysoja_032687 PE=4 S...	[more]
A0A151RB82_CAJCA	1.4e-37	58.33	Uncharacterized protein OS=Cajanus cajan GN=KK1_039066 PE=4 SV=1	[more]

BLAST of CSPI03G30280 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G80920.1	4.1e-33	56.74	Chaperone DnaJ-domain superfamily protein	[more]
AT1G72070.1	8.4e-10	37.50	Chaperone DnaJ-domain superfamily protein	[more]
AT5G59610.1	7.9e-08	36.11	Chaperone DnaJ-domain superfamily protein	[more]
AT3G08970.1	1.1e-06	40.38	DNAJ heat shock N-terminal domain-containing protein	[more]
AT4G21180.1	1.9e-06	32.97	DnaJ / Sec63 Brl domains-containing protein	[more]

BLAST of CSPI03G30280 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778682940\|ref\|XP_004137653.2\|	3.1e-83	98.72	PREDICTED: chaperone protein dnaJ 8, chloroplastic-like [Cucumis sativus]	[more]
gi\|778682937\|ref\|XP_011651813.1\|	9.5e-72	91.77	PREDICTED: chaperone protein dnaJ 8, chloroplastic-like [Cucumis sativus]	[more]
gi\|659130374\|ref\|XP_008465135.1\|	4.3e-56	77.71	PREDICTED: chaperone protein dnaJ 8, chloroplastic [Cucumis melo]	[more]
gi\|595923676\|ref\|XP_007214842.1\|	1.4e-38	62.34	hypothetical protein PRUPE_ppa012860mg [Prunus persica]	[more]
gi\|1021570934\|ref\|XP_016176636.1\|	3.1e-38	60.90	PREDICTED: chaperone protein dnaJ 8, chloroplastic-like [Arachis ipaensis]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR001623	DnaJ_domain

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0043231	intracellular membrane-bounded organelle
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G30280.1	CSPI03G30280.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001623	DnaJ domain	PRINTS	PR00625	JDOMAIN	coord: 51..69 score: 2.0E-10coord: 85..105 score: 2.0E-10coord: 69..84 score: 2.0
IPR001623	DnaJ domain	GENE3D	G3DSA:1.10.287.110		coord: 43..102 score: 9.1
IPR001623	DnaJ domain	PFAM	PF00226	DnaJ	coord: 49..102 score: 2.9
IPR001623	DnaJ domain	SMART	SM00271	dnaj_3	coord: 48..105 score: 1.2
IPR001623	DnaJ domain	PROFILE	PS50076	DNAJ_2	coord: 49..119 score: 13
IPR001623	DnaJ domain	unknown	SSF46565	Chaperone J-domain	coord: 40..103 score: 1.31
None	No IPR available	PANTHER	PTHR24078	DNAJ HOMOLOG SUBFAMILY C MEMBER	coord: 49..97 score: 1.0
None	No IPR available	PANTHER	PTHR24078:SF231	CHAPERONE PROTEIN DNAJ	coord: 49..97 score: 1.0

Synteny

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CSPI03G30280	Wax gourd	cpiwgoB221
CSPI03G30280	Wild cucumber (PI 183967)	cpicpiB142
CSPI03G30280	Cucumber (Gy14) v1	cgycpiB278
CSPI03G30280	Cucurbita maxima (Rimu)	cmacpiB243
CSPI03G30280	Cucurbita maxima (Rimu)	cmacpiB456
CSPI03G30280	Cucurbita moschata (Rifu)	cmocpiB231
CSPI03G30280	Cucurbita moschata (Rifu)	cmocpiB356
CSPI03G30280	Cucurbita moschata (Rifu)	cmocpiB444
CSPI03G30280	Cucumber (Chinese Long) v2	cpicuB120
CSPI03G30280	Melon (DHL92) v3.5.1	cpimeB199
CSPI03G30280	Watermelon (Charleston Gray)	cpiwcgB188
CSPI03G30280	Watermelon (Charleston Gray)	cpiwcgB191
CSPI03G30280	Watermelon (97103) v1	cpiwmB192
CSPI03G30280	Watermelon (97103) v1	cpiwmB194
CSPI03G30280	Cucurbita pepo (Zucchini)	cpecpiB138
CSPI03G30280	Cucurbita pepo (Zucchini)	cpecpiB546
CSPI03G30280	Cucurbita pepo (Zucchini)	cpecpiB601
CSPI03G30280	Bottle gourd (USVL1VR-Ls)	cpilsiB171
CSPI03G30280	Bottle gourd (USVL1VR-Ls)	cpilsiB181
CSPI03G30280	Melon (DHL92) v3.6.1	cpimedB174
CSPI03G30280	Melon (DHL92) v3.6.1	cpimedB193
CSPI03G30280	Cucumber (Gy14) v2	cgybcpiB110
CSPI03G30280	Cucumber (Gy14) v2	cgybcpiB313
CSPI03G30280	Silver-seed gourd	carcpiB0227
CSPI03G30280	Silver-seed gourd	carcpiB0390
CSPI03G30280	Silver-seed gourd	carcpiB0850
CSPI03G30280	Cucumber (Chinese Long) v3	cpicucB144
CSPI03G30280	Cucumber (Chinese Long) v3	cpicucB191
CSPI03G30280	Watermelon (97103) v2	cpiwmbB185
CSPI03G30280	Watermelon (97103) v2	cpiwmbB268