CSPI03G28140 (gene) Wild cucumber (PI 183967) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CSPI03G28140
Type	gene
Organism	Cucumis sativus (Wild cucumber (PI 183967))
Description	Mediator of RNA polymerase II transcription subunit 8, putative
Location	Chr3 : 25743080 .. 25747263 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

AATTTATTTTATCAACGCCACTTTATAAGAAAGAACATGAGAATCAAATAGTTATTGAAAATTACATTTAATTTGGGATCAAATAGATATACGCTTCCAGGTAAAACGGTATCAATCGGAGCACCTCAGGCAAGCCTGGCCTCGCAGCCAGACCCGAGCGAGATCTACACTCCCCGTACCCGTTGTACGTTACTCGCATCGAACAGGCCAACTGGTGTCTTTCGCTAATGGCGACCATTGACGGGGCGGTGCAGAATCAGCCTCAGCAGCAACAGCCTCTTCCGGCGCAGCAGGTGGTTTTCGCGGAAAGGCTAAACCAGGCAGTTCAGCAACAGTTGAACCTGGAGGCTCTGAAGAATCGAGCTACTACTCTCTTTAAAGCCATTTCTCGGATCCTTGAAGATTTTGATGTCTATGGTCGCACCAATACCACACCTAAATGGTCTACTTTCATTTTTCGCCTAGTTTTCTCTTGTGATATTTTCCAAATATGCTTGAATTTTTATGTTCGTCATTGGCGATTGATAGCACGTTAGTGTTAGAAAGTAATTTTTTTCTCTATCTCGTGGATTTACATACTGATTATTTTCTAGACATCAAAGCTTCTTAATATCTTTCTATCTATCTTTCTATTTATATTTCGATATTCTCCTCTACTGGCCGACTGTATAGTTGATTTTTACGCCAGGCAAGACATACTGGGGCAGTATTCTATGGTGAATCTCGAGCTTTTCAACATTGTTGACGAAATCAAGAAAGTTTCGAAGGCTTTCGTTGTACATCCAAAGAATGTTAATGCAGAGAACTCTACAAGTATGTCCCTGCATCCCTTCCAAGTATTTGCTGAAGTAAAAGGAAGAAATGCTTAGTTTTTGTTTTGTACGCTTGAACAATCATTCATTTATTTGTAAATTTTGATTTCAACTAATTTTTCTTTCTTTTTTCTTTTTGTGAGCCCAGTACTACCAGTTATGTTGTCCTCAAAACTATTGCCTGAGATGGAGGTAGATGACAATTCGAAACGAGAGCAGTTGCTTCTTGGGATGCACAACATGCCAGTATCTACACAGATTGAGAAATTAAAGGTACTAGAAGTTTGTTAAATAATTATAGGTCGTAGAACTCCCCGTGTTCTAAAGAAATTCATTAAATCACATACTTTCTTATGTATCTGGCATTTAGGTTTACTTTTCTTACAGACTAGAATTGAAATGATTGGAGCAGCCTGTGAAAGTGCTGAAAAAATATTAGCTGAAACTCGAAAAGCATATTGCTTCGGAATGCGTCAAGGGCCAGCAATAGCTCCAACCTTAGATAAGGCTCAAGCTGCAAAAATTCAGGAGCAAGAAAATTTACTCCGAGCTGCTGTTAACTTTGGGGAAGGTGATATTTTTTTACTTTTATAGTTATTGGTTAGTATCTTAGTTTTAATTATTTGTAGTTCATATATTGTTTAGTACTGCATCATGGCTTGTAAGTCCACTCATGATCGACATTTACCAAAAAAAGCAATATTATATTTAGACTTCTTGCCAACTTGTCACATATCTATGAAGATTACTGATTTGTATATTTATTTCTTTATTCATGATATCTTTATCAATTTTTTTGTGCATAATTGAATATGAACATTCTAACTTGTGTAACAGCCATTTCTCCATTGTTGTATGAATTAGACACAATAACTTGCTTTTTGGGAGAGTTGATTTGAATGACTAAATTGTTTGAAATTTTGTGAAAGAGTTGCTGAGGGAGAAAGGAGAAGTGGAGTGTTGTAGAATGGGAGTGATGCGACCTCGTAAAATGACCATATGAATTATGGAGGATGCATATATGGCAAAAAATGCTCTGTTCCTTCGGTCAATGAGGCTGTAGGGATCCTAGCTTAGTTGGATCCCCTAAACCAATTGATCTATCATCCTTCTTCATCCTTCCCAAACACTTGAAGGCCCCAACTAAGTAGCAAGCCCACACTATGAGGCTTGGAAAACTGATTTAATTCTTGCAAAAGATGTGGGTATAAGGTGTTAAAAGTAATATCTGAAAAAGAGGAACCATTATGGATTGACCTAATGGTAAATAGGGGACATGACTTTGATAAAGGGTTGAGAGGTCACAGGCTCAGTCCCTGATCTACCTAGCTAGAATTTAGTGTCCTATGAGTTTTCTCGACATCCAAATGTTGTAGGGTTAAATCAATTGTTTTGTGAGATTAGTCGAGATGTGCATAAGCTGATCTAAACCCTTACAAATTTCAAAAAAAAAAAAAAGAAAAGAAAAGAAAAAGAGGGTTGTCAACTAGTAATCTTTTCTATTTGAAGAGGCTTGTTTCCATTTAAAAAAAAAACAAGTAGCCTTTTCTATTAAATTCCAAGATCTAATATTCTCCTTCAAATATCTCTCTTGCAAAAATTCTCAACACACAATAGAATGCAAGATCTTCAATCATTTCCTCCGTACTGTTTCATTTTTCTCTCTTTTGTGCTCTTTAGAGCAAATGGCCAAAAGGGAGATGTTCTTTGTTGTCAAGCTTTGAGCCTAATGTAATAGTTGCAAAGTGGTTTGAGCTACAAACATTTCAGTTGTGTGATTGTATCTTGGAGGAGACATGTTAGACAATATCTGGTGCCTTGGTATAAAGGAATACGTAGTCTCTTAAAAGACAGTGAGATACATGCACCTCAATGTAATTGTTTACGCGTTTGTTTCTCAATATTTACTCCAATTTCTAGGTTATTTGTAAACCATTAGTTCGTTAGTTAAGCTGTTGGGCTTCGGCAAGACAAAAGGCTCATCAGATCCTTCGTTAGATTCCATATTTCTCTCAGTTTCAGTTACAAAAGTTTTTTGTATTGACAATAGAGCATTATTTTACGCAACTGATGGCCCTTTCTTTAGTGGCCTACCTTTATGTAGGATTATTCTTTTTGGATCCTGGTAAATTCTTTCATTTTTTTTTCTCAAATGTTGTTTTCTCATTATAAAGAAAAATAAAATAAAATAAAATAAAAGAGAAGTCCTTGATGCCGATGCATATGATTTATAACATTATTTTTTGGTTTACTTGGATCTATTGTTAAAACTTGGCTCAAACTTGAAGTTTCATTTGCTTCTATATTTGTCCTCTCCTAATTTTTTCAATAAGTGCAGTAGTCATGCCTTGTAAATGAATCTTATATTTACATGCAGGATTAAGATTACCTGGTGAGCAACGACAAATTACGCCAACACTTCCGATGCATTTGATAGATGTGCTGAATGCAGGTGATGGAGTGCAGAGTTTTAACGAAACGTCCGGTAACGTTCATTTGTCTTTTCAAACATAAATGGTTTATAGATTAATTTACTAAATTGCACTAACTTGTAATTGTTTAACAAAATCTCTTTCAGGTATGTATGCAAAGAGTACTCCTCTAACGTCAAATAATATTAGTAGTCAAGGAGCAGTGATACAGGTTAGATGATGTCCTTGCTCCTTGAAATGGTTTCTTAATGATTTATTTGCATCTTCTTAAGTTATAGAATGTAAACTAAGTTCTCCAAGGGGAAGCAACTTTTGGCTAACAGATTTCGTTTCATTCGAGGTTCTCTTATGTACGTGATTGAATTATCTTGTATCAGTTGGTTCCTAATTTTATTAGGATTTCTTTGGTTATGACTTATGAGTTGTATCTACCATTCATTCTTCCTTGAAAGATGACGTTAGATAGTATAGTACTGTTAGGAATAAAATAATTGAACATTTTCTTGCCGACTGTCTCCTATTCTCGCAATTCTTTCTTTTCCCTGCTCTTCCTATGATTATTTGCATTATTATTGGTGTTGAACTCGGGCACTTGGATTGTTTGGTTTGCACATTTAGATATAATAAGAACTCTACATGTTATTTTTGAGGCTAATGTATGGATCAAAATTAATAGGGTAATGATAGAACACCAAGTAGTGTGTTCTATTATTTTATTAAACAACCAAACTTTTACAGAAAATAGTACTAACACAACCTTGAAAACAAAGAACAACACAAGAACTAATATAGAAATGAACCAAGTTCAACATAAAACATCAAAACAGAAAAGTACTTCACGATAATGGAAATAACTTGCAACAGACTACAAAAACTGAAAACTTTTTGCATTCCCTGCTCTCCAGGAAATTACAGCCCTCCACTTCCAGAAACTGATTAA

mRNA sequence

ATGGCGACCATTGACGGGGCGGTGCAGAATCAGCCTCAGCAGCAACAGCCTCTTCCGGCGCAGCAGGTGGTTTTCGCGGAAAGGCTAAACCAGGCAGTTCAGCAACAGTTGAACCTGGAGGCTCTGAAGAATCGAGCTACTACTCTCTTTAAAGCCATTTCTCGGATCCTTGAAGATTTTGATGTCTATGGTCGCACCAATACCACACCTAAATGGCAAGACATACTGGGGCAGTATTCTATGGTGAATCTCGAGCTTTTCAACATTGTTGACGAAATCAAGAAAGTTTCGAAGGCTTTCGTTGTACATCCAAAGAATGTTAATGCAGAGAACTCTACAATACTACCAGTTATGTTGTCCTCAAAACTATTGCCTGAGATGGAGGTAGATGACAATTCGAAACGAGAGCAGTTGCTTCTTGGGATGCACAACATGCCAGTATCTACACAGATTGAGAAATTAAAGACTAGAATTGAAATGATTGGAGCAGCCTGTGAAAGTGCTGAAAAAATATTAGCTGAAACTCGAAAAGCATATTGCTTCGGAATGCGTCAAGGGCCAGCAATAGCTCCAACCTTAGATAAGGCTCAAGCTGCAAAAATTCAGGAGCAAGAAAATTTACTCCGAGCTGCTGTTAACTTTGGGGAAGGATTAAGATTACCTGGTGAGCAACGACAAATTACGCCAACACTTCCGATGCATTTGATAGATGTGCTGAATGCAGGTGATGGAGTGCAGAGTTTTAACGAAACGTCCGGTATGTATGCAAAGAGTACTCCTCTAACGTCAAATAATATTAGTAGTCAAGGAGCAGTGATACAGGGGAAGCAACTTTTGGCTAACAGATTTCGTTTCATTCGAGGAAATTACAGCCCTCCACTTCCAGAAACTGATTAA

Coding sequence (CDS)

ATGGCGACCATTGACGGGGCGGTGCAGAATCAGCCTCAGCAGCAACAGCCTCTTCCGGCGCAGCAGGTGGTTTTCGCGGAAAGGCTAAACCAGGCAGTTCAGCAACAGTTGAACCTGGAGGCTCTGAAGAATCGAGCTACTACTCTCTTTAAAGCCATTTCTCGGATCCTTGAAGATTTTGATGTCTATGGTCGCACCAATACCACACCTAAATGGCAAGACATACTGGGGCAGTATTCTATGGTGAATCTCGAGCTTTTCAACATTGTTGACGAAATCAAGAAAGTTTCGAAGGCTTTCGTTGTACATCCAAAGAATGTTAATGCAGAGAACTCTACAATACTACCAGTTATGTTGTCCTCAAAACTATTGCCTGAGATGGAGGTAGATGACAATTCGAAACGAGAGCAGTTGCTTCTTGGGATGCACAACATGCCAGTATCTACACAGATTGAGAAATTAAAGACTAGAATTGAAATGATTGGAGCAGCCTGTGAAAGTGCTGAAAAAATATTAGCTGAAACTCGAAAAGCATATTGCTTCGGAATGCGTCAAGGGCCAGCAATAGCTCCAACCTTAGATAAGGCTCAAGCTGCAAAAATTCAGGAGCAAGAAAATTTACTCCGAGCTGCTGTTAACTTTGGGGAAGGATTAAGATTACCTGGTGAGCAACGACAAATTACGCCAACACTTCCGATGCATTTGATAGATGTGCTGAATGCAGGTGATGGAGTGCAGAGTTTTAACGAAACGTCCGGTATGTATGCAAAGAGTACTCCTCTAACGTCAAATAATATTAGTAGTCAAGGAGCAGTGATACAGGGGAAGCAACTTTTGGCTAACAGATTTCGTTTCATTCGAGGAAATTACAGCCCTCCACTTCCAGAAACTGATTAA

Homology

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BLAST of CSPI03G28140 vs. Swiss-Prot
Match: MED8_ARATH (Mediator of RNA polymerase II transcription subunit 8 OS=Arabidopsis thaliana GN=MED8 PE=1 SV=1)

HSP 1 Score: 320.1 bits (819), Expect = 2.6e-86
Identity = 176/293 (60.07%), Postives = 216/293 (73.72%), Query Frame = 1


Query: 8   VQNQPQQQQPLPAQQVVFAERLNQAVQQQLNLEALKNRATTLFKAISRILEDFDVYGRTN 67
           ++ QPQQ  P P      AE+LN  ++++LNLE+LK RA +L KAI+RILEDFD YGRTN
Sbjct: 1   METQPQQPPPPPV-----AEKLNPKLEKELNLESLKTRAVSLAKAIARILEDFDAYGRTN 60

Query: 68  TTPKWQDILGQYSMVNLELFNIVDEIKKVSKAFVVHPKNVNAENSTILPVMLSSKLLPEM 127
           TTPKWQDILGQYSMVNLELFNIV+E+K+VS AFVV PKNVNA N+ ILPVMLSSKLLPEM
Sbjct: 61  TTPKWQDILGQYSMVNLELFNIVEEVKRVSNAFVVLPKNVNAMNAAILPVMLSSKLLPEM 120

Query: 128 EVDDNSKREQLLLGMHNMPVSTQIEKLKTRIEMIGAACESAEKILAETRKAYCFGMRQGP 187
           E DDN+KREQLL G+ ++P+  QIE+LK R++MI AACE+AE++LA+TRKAY FG RQGP
Sbjct: 121 ETDDNAKREQLLQGVQSLPIPMQIERLKARMDMIAAACENAERVLADTRKAYGFGTRQGP 180

Query: 188 AIAPTLDKAQAAKIQEQENLLRAAVNFGEGLRLPGEQRQITPTLPMHLIDVLNAGDGVQS 247
           ++ PT+DK QAAKIQEQE +LR AVN G+G +LP +QRQIT  LP HL DVL   D    
Sbjct: 181 SMLPTMDKGQAAKIQEQEKMLRDAVNDGKGTQLPPDQRQITTALPPHLADVLIIND---- 240

Query: 248 FNETSGMYAKSTPLTSNNISSQGAV-IQGKQLLANRFRFIRG----NYSPPLP 296
               +G  A   P  SNNI++QG + + G Q +        G    N + PLP
Sbjct: 241 ----AGKIA--LPGQSNNINNQGMMQVSGTQFVGRSAASPSGPNFDNTTSPLP 278

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BLAST of CSPI03G28140 vs. TrEMBL
Match: A0A0A0L8Z2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G640620 PE=4 SV=1)

HSP 1 Score: 529.3 bits (1362), Expect = 3.1e-147
Identity = 274/274 (100.00%), Postives = 274/274 (100.00%), Query Frame = 1


Query: 1   MATIDGAVQNQPQQQQPLPAQQVVFAERLNQAVQQQLNLEALKNRATTLFKAISRILEDF 60
           MATIDGAVQNQPQQQQPLPAQQVVFAERLNQAVQQQLNLEALKNRATTLFKAISRILEDF
Sbjct: 1   MATIDGAVQNQPQQQQPLPAQQVVFAERLNQAVQQQLNLEALKNRATTLFKAISRILEDF 60

Query: 61  DVYGRTNTTPKWQDILGQYSMVNLELFNIVDEIKKVSKAFVVHPKNVNAENSTILPVMLS 120
           DVYGRTNTTPKWQDILGQYSMVNLELFNIVDEIKKVSKAFVVHPKNVNAENSTILPVMLS
Sbjct: 61  DVYGRTNTTPKWQDILGQYSMVNLELFNIVDEIKKVSKAFVVHPKNVNAENSTILPVMLS 120

Query: 121 SKLLPEMEVDDNSKREQLLLGMHNMPVSTQIEKLKTRIEMIGAACESAEKILAETRKAYC 180
           SKLLPEMEVDDNSKREQLLLGMHNMPVSTQIEKLKTRIEMIGAACESAEKILAETRKAYC
Sbjct: 121 SKLLPEMEVDDNSKREQLLLGMHNMPVSTQIEKLKTRIEMIGAACESAEKILAETRKAYC 180

Query: 181 FGMRQGPAIAPTLDKAQAAKIQEQENLLRAAVNFGEGLRLPGEQRQITPTLPMHLIDVLN 240
           FGMRQGPAIAPTLDKAQAAKIQEQENLLRAAVNFGEGLRLPGEQRQITPTLPMHLIDVLN
Sbjct: 181 FGMRQGPAIAPTLDKAQAAKIQEQENLLRAAVNFGEGLRLPGEQRQITPTLPMHLIDVLN 240

Query: 241 AGDGVQSFNETSGMYAKSTPLTSNNISSQGAVIQ 275
           AGDGVQSFNETSGMYAKSTPLTSNNISSQGAVIQ
Sbjct: 241 AGDGVQSFNETSGMYAKSTPLTSNNISSQGAVIQ 274

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BLAST of CSPI03G28140 vs. TrEMBL
Match: A0A061GA94_THECC (Mediator subunit 8 isoform 1 OS=Theobroma cacao GN=TCM_027824 PE=4 SV=1)

HSP 1 Score: 418.7 bits (1075), Expect = 6.0e-114
Identity = 216/279 (77.42%), Postives = 244/279 (87.46%), Query Frame = 1


Query: 4   IDGAVQNQPQQQQPLPAQQVVF-AERLNQAVQQQLNLEALKNRATTLFKAISRILEDFDV 63
           ++G +Q+  QQQQ     QVV  AERLNQAVQQQLNLE+LK RA +LFKAISRILEDFD 
Sbjct: 1   MEGMIQDPSQQQQAQQKNQVVAGAERLNQAVQQQLNLESLKTRAISLFKAISRILEDFDA 60

Query: 64  YGRTNTTPKWQDILGQYSMVNLELFNIVDEIKKVSKAFVVHPKNVNAENSTILPVMLSSK 123
           Y RTNTTPKW DILGQYSMVNLELFNIVDEIKKVSKAFVVHPKNVNAEN++ILPVMLSSK
Sbjct: 61  YSRTNTTPKWLDILGQYSMVNLELFNIVDEIKKVSKAFVVHPKNVNAENASILPVMLSSK 120

Query: 124 LLPEMEVDDNSKREQLLLGMHNMPVSTQIEKLKTRIEMIGAACESAEKILAETRKAYCFG 183
           LLPEME +DN KREQLL GM N+P+ +QIEKLKTRI+MIGAACESAEK+LA+TRKAYCFG
Sbjct: 121 LLPEMETEDNLKREQLLQGMQNLPIPSQIEKLKTRIDMIGAACESAEKVLADTRKAYCFG 180

Query: 184 MRQGPAIAPTLDKAQAAKIQEQENLLRAAVNFGEGLRLPGEQRQITPTLPMHLIDVLNAG 243
            RQGPAI PTLDK QAAKIQEQENLLRAAVNFGEGL LP +QRQITP+LP+HL D++ A 
Sbjct: 181 SRQGPAILPTLDKGQAAKIQEQENLLRAAVNFGEGLHLPADQRQITPSLPLHLADIMPAA 240

Query: 244 DGVQSFNETSGMYAKSTPLTSNNISSQGAVIQ--GKQLL 280
           DGV SF++ SGMY K+TPLTS+NI SQG+++Q  G QL+
Sbjct: 241 DGVHSFSDPSGMYMKNTPLTSSNIGSQGSLVQASGAQLI 279

[Close]

BLAST of CSPI03G28140 vs. TrEMBL
Match: A0A061GGZ8_THECC (Mediator subunit 8 isoform 2 OS=Theobroma cacao GN=TCM_027824 PE=4 SV=1)

HSP 1 Score: 418.7 bits (1075), Expect = 6.0e-114
Identity = 216/279 (77.42%), Postives = 244/279 (87.46%), Query Frame = 1


Query: 4   IDGAVQNQPQQQQPLPAQQVVF-AERLNQAVQQQLNLEALKNRATTLFKAISRILEDFDV 63
           ++G +Q+  QQQQ     QVV  AERLNQAVQQQLNLE+LK RA +LFKAISRILEDFD 
Sbjct: 1   MEGMIQDPSQQQQAQQKNQVVAGAERLNQAVQQQLNLESLKTRAISLFKAISRILEDFDA 60

Query: 64  YGRTNTTPKWQDILGQYSMVNLELFNIVDEIKKVSKAFVVHPKNVNAENSTILPVMLSSK 123
           Y RTNTTPKW DILGQYSMVNLELFNIVDEIKKVSKAFVVHPKNVNAEN++ILPVMLSSK
Sbjct: 61  YSRTNTTPKWLDILGQYSMVNLELFNIVDEIKKVSKAFVVHPKNVNAENASILPVMLSSK 120

Query: 124 LLPEMEVDDNSKREQLLLGMHNMPVSTQIEKLKTRIEMIGAACESAEKILAETRKAYCFG 183
           LLPEME +DN KREQLL GM N+P+ +QIEKLKTRI+MIGAACESAEK+LA+TRKAYCFG
Sbjct: 121 LLPEMETEDNLKREQLLQGMQNLPIPSQIEKLKTRIDMIGAACESAEKVLADTRKAYCFG 180

Query: 184 MRQGPAIAPTLDKAQAAKIQEQENLLRAAVNFGEGLRLPGEQRQITPTLPMHLIDVLNAG 243
            RQGPAI PTLDK QAAKIQEQENLLRAAVNFGEGL LP +QRQITP+LP+HL D++ A 
Sbjct: 181 SRQGPAILPTLDKGQAAKIQEQENLLRAAVNFGEGLHLPADQRQITPSLPLHLADIMPAA 240

Query: 244 DGVQSFNETSGMYAKSTPLTSNNISSQGAVIQ--GKQLL 280
           DGV SF++ SGMY K+TPLTS+NI SQG+++Q  G QL+
Sbjct: 241 DGVHSFSDPSGMYMKNTPLTSSNIGSQGSLVQASGAQLI 279

[Close]

BLAST of CSPI03G28140 vs. TrEMBL
Match: A0A061G8Z4_THECC (Mediator subunit 8 isoform 3 OS=Theobroma cacao GN=TCM_027824 PE=4 SV=1)

HSP 1 Score: 417.2 bits (1071), Expect = 1.7e-113
Identity = 213/272 (78.31%), Postives = 240/272 (88.24%), Query Frame = 1


Query: 4   IDGAVQNQPQQQQPLPAQQVVF-AERLNQAVQQQLNLEALKNRATTLFKAISRILEDFDV 63
           ++G +Q+  QQQQ     QVV  AERLNQAVQQQLNLE+LK RA +LFKAISRILEDFD 
Sbjct: 1   MEGMIQDPSQQQQAQQKNQVVAGAERLNQAVQQQLNLESLKTRAISLFKAISRILEDFDA 60

Query: 64  YGRTNTTPKWQDILGQYSMVNLELFNIVDEIKKVSKAFVVHPKNVNAENSTILPVMLSSK 123
           Y RTNTTPKW DILGQYSMVNLELFNIVDEIKKVSKAFVVHPKNVNAEN++ILPVMLSSK
Sbjct: 61  YSRTNTTPKWLDILGQYSMVNLELFNIVDEIKKVSKAFVVHPKNVNAENASILPVMLSSK 120

Query: 124 LLPEMEVDDNSKREQLLLGMHNMPVSTQIEKLKTRIEMIGAACESAEKILAETRKAYCFG 183
           LLPEME +DN KREQLL GM N+P+ +QIEKLKTRI+MIGAACESAEK+LA+TRKAYCFG
Sbjct: 121 LLPEMETEDNLKREQLLQGMQNLPIPSQIEKLKTRIDMIGAACESAEKVLADTRKAYCFG 180

Query: 184 MRQGPAIAPTLDKAQAAKIQEQENLLRAAVNFGEGLRLPGEQRQITPTLPMHLIDVLNAG 243
            RQGPAI PTLDK QAAKIQEQENLLRAAVNFGEGL LP +QRQITP+LP+HL D++ A 
Sbjct: 181 SRQGPAILPTLDKGQAAKIQEQENLLRAAVNFGEGLHLPADQRQITPSLPLHLADIMPAA 240

Query: 244 DGVQSFNETSGMYAKSTPLTSNNISSQGAVIQ 275
           DGV SF++ SGMY K+TPLTS+NI SQG+++Q
Sbjct: 241 DGVHSFSDPSGMYMKNTPLTSSNIGSQGSLVQ 272

[Close]

BLAST of CSPI03G28140 vs. TrEMBL
Match: V4UNK2_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10025265mg PE=4 SV=1)

HSP 1 Score: 415.2 bits (1066), Expect = 6.6e-113
Identity = 217/287 (75.61%), Postives = 245/287 (85.37%), Query Frame = 1


Query: 1   MATIDGAV-----QNQPQQQQPLPAQQVVFAERLNQAVQQQLNLEALKNRATTLFKAISR 60
           MA ++G       Q Q QQQQ L   Q V  ERLNQAV QQLNLEA+K RA +LFKAISR
Sbjct: 1   MAAMEGVAMQDQSQQQQQQQQQLVPPQPVAVERLNQAVVQQLNLEAVKTRAISLFKAISR 60

Query: 61  ILEDFDVYGRTNTTPKWQDILGQYSMVNLELFNIVDEIKKVSKAFVVHPKNVNAENSTIL 120
           ILEDFD Y RTNTTPKWQDILGQYSMVNLELFNIVDEI+KVSKAFVVHPKNVNAEN+TIL
Sbjct: 61  ILEDFDAYARTNTTPKWQDILGQYSMVNLELFNIVDEIRKVSKAFVVHPKNVNAENATIL 120

Query: 121 PVMLSSKLLPEMEVDDNSKREQLLLGMHNMPVSTQIEKLKTRIEMIGAACESAEKILAET 180
           PVMLSSKLLPEME+DDNSKREQLLLGM N+P+ +QIEKLK+RI+MIGAACESAEK+LA+T
Sbjct: 121 PVMLSSKLLPEMEIDDNSKREQLLLGMQNLPIPSQIEKLKSRIDMIGAACESAEKVLADT 180

Query: 181 RKAYCFGMRQGPAIAPTLDKAQAAKIQEQENLLRAAVNFGEGLRLPGEQRQITPTLPMHL 240
           RKAYCFG RQGP I PTLDK QA KIQEQENLLRAAVN GEGLRLPG+QRQ+TP LPMHL
Sbjct: 181 RKAYCFGTRQGPQILPTLDKGQALKIQEQENLLRAAVNSGEGLRLPGDQRQMTPALPMHL 240

Query: 241 IDVLNAGDGVQSFNETSGMYAKST-PLTSNNISSQGAVIQ--GKQLL 280
           +D+L  GDGV +F++ SGMY K+T PL+SN+I +QG ++Q  G QL+
Sbjct: 241 VDLLPVGDGVHTFSDASGMYMKNTPPLSSNSIGNQGNLLQASGAQLI 287

[Close]

BLAST of CSPI03G28140 vs. TAIR10
Match: AT2G03070.1 (AT2G03070.1 mediator subunit 8)

HSP 1 Score: 320.1 bits (819), Expect = 1.5e-87
Identity = 176/293 (60.07%), Postives = 216/293 (73.72%), Query Frame = 1


Query: 8   VQNQPQQQQPLPAQQVVFAERLNQAVQQQLNLEALKNRATTLFKAISRILEDFDVYGRTN 67
           ++ QPQQ  P P      AE+LN  ++++LNLE+LK RA +L KAI+RILEDFD YGRTN
Sbjct: 1   METQPQQPPPPPV-----AEKLNPKLEKELNLESLKTRAVSLAKAIARILEDFDAYGRTN 60

Query: 68  TTPKWQDILGQYSMVNLELFNIVDEIKKVSKAFVVHPKNVNAENSTILPVMLSSKLLPEM 127
           TTPKWQDILGQYSMVNLELFNIV+E+K+VS AFVV PKNVNA N+ ILPVMLSSKLLPEM
Sbjct: 61  TTPKWQDILGQYSMVNLELFNIVEEVKRVSNAFVVLPKNVNAMNAAILPVMLSSKLLPEM 120

Query: 128 EVDDNSKREQLLLGMHNMPVSTQIEKLKTRIEMIGAACESAEKILAETRKAYCFGMRQGP 187
           E DDN+KREQLL G+ ++P+  QIE+LK R++MI AACE+AE++LA+TRKAY FG RQGP
Sbjct: 121 ETDDNAKREQLLQGVQSLPIPMQIERLKARMDMIAAACENAERVLADTRKAYGFGTRQGP 180

Query: 188 AIAPTLDKAQAAKIQEQENLLRAAVNFGEGLRLPGEQRQITPTLPMHLIDVLNAGDGVQS 247
           ++ PT+DK QAAKIQEQE +LR AVN G+G +LP +QRQIT  LP HL DVL   D    
Sbjct: 181 SMLPTMDKGQAAKIQEQEKMLRDAVNDGKGTQLPPDQRQITTALPPHLADVLIIND---- 240

Query: 248 FNETSGMYAKSTPLTSNNISSQGAV-IQGKQLLANRFRFIRG----NYSPPLP 296
               +G  A   P  SNNI++QG + + G Q +        G    N + PLP
Sbjct: 241 ----AGKIA--LPGQSNNINNQGMMQVSGTQFVGRSAASPSGPNFDNTTSPLP 278

[Close]

BLAST of CSPI03G28140 vs. NCBI nr
Match: gi|449453344|ref|XP_004144418.1| (PREDICTED: mediator of RNA polymerase II transcription subunit 8 isoform X2 [Cucumis sativus])

HSP 1 Score: 530.4 bits (1365), Expect = 2.0e-147
Identity = 277/281 (98.58%), Postives = 278/281 (98.93%), Query Frame = 1


Query: 1   MATIDGAVQNQPQQQQPLPAQQVVFAERLNQAVQQQLNLEALKNRATTLFKAISRILEDF 60
           MATIDGAVQNQPQQQQPLPAQQVVFAERLNQAVQQQLNLEALKNRATTLFKAISRILEDF
Sbjct: 1   MATIDGAVQNQPQQQQPLPAQQVVFAERLNQAVQQQLNLEALKNRATTLFKAISRILEDF 60

Query: 61  DVYGRTNTTPKWQDILGQYSMVNLELFNIVDEIKKVSKAFVVHPKNVNAENSTILPVMLS 120
           DVYGRTNTTPKWQDILGQYSMVNLELFNIVDEIKKVSKAFVVHPKNVNAENSTILPVMLS
Sbjct: 61  DVYGRTNTTPKWQDILGQYSMVNLELFNIVDEIKKVSKAFVVHPKNVNAENSTILPVMLS 120

Query: 121 SKLLPEMEVDDNSKREQLLLGMHNMPVSTQIEKLKTRIEMIGAACESAEKILAETRKAYC 180
           SKLLPEMEVDDNSKREQLLLGMHNMPVSTQIEKLKTRIEMIGAACESAEKILAETRKAYC
Sbjct: 121 SKLLPEMEVDDNSKREQLLLGMHNMPVSTQIEKLKTRIEMIGAACESAEKILAETRKAYC 180

Query: 181 FGMRQGPAIAPTLDKAQAAKIQEQENLLRAAVNFGEGLRLPGEQRQITPTLPMHLIDVLN 240
           FGMRQGPAIAPTLDKAQAAKIQEQENLLRAAVNFGEGLRLPGEQRQITPTLPMHLIDVLN
Sbjct: 181 FGMRQGPAIAPTLDKAQAAKIQEQENLLRAAVNFGEGLRLPGEQRQITPTLPMHLIDVLN 240

Query: 241 AGDGVQSFNETSGMYAKSTPLTSNNISSQGAVIQ--GKQLL 280
           AGDGVQSFNETSGMYAKSTPLTSNNISSQGAVIQ  G QL+
Sbjct: 241 AGDGVQSFNETSGMYAKSTPLTSNNISSQGAVIQPPGSQLV 281

[Close]

BLAST of CSPI03G28140 vs. NCBI nr
Match: gi|778682360|ref|XP_011651696.1| (PREDICTED: mediator of RNA polymerase II transcription subunit 8 isoform X1 [Cucumis sativus])

HSP 1 Score: 530.0 bits (1364), Expect = 2.6e-147
Identity = 277/282 (98.23%), Postives = 278/282 (98.58%), Query Frame = 1


Query: 1   MATIDGAVQNQPQQQQPLPAQQVVFAERLNQAVQQQLNLEALKNRATTLFKAISRILEDF 60
           MATIDGAVQNQPQQQQPLPAQQVVFAERLNQAVQQQLNLEALKNRATTLFKAISRILEDF
Sbjct: 1   MATIDGAVQNQPQQQQPLPAQQVVFAERLNQAVQQQLNLEALKNRATTLFKAISRILEDF 60

Query: 61  DVYGRTNTTPKWQDILGQYSMVNLELFNIVDEIKKVSKAFVVHPKNVNAENSTILPVMLS 120
           DVYGRTNTTPKWQDILGQYSMVNLELFNIVDEIKKVSKAFVVHPKNVNAENSTILPVMLS
Sbjct: 61  DVYGRTNTTPKWQDILGQYSMVNLELFNIVDEIKKVSKAFVVHPKNVNAENSTILPVMLS 120

Query: 121 SKLLPEMEVDDNSKREQLLLGMHNMPVSTQIEKLKTRIEMIGAACESAEKILAETRKAYC 180
           SKLLPEMEVDDNSKREQLLLGMHNMPVSTQIEKLKTRIEMIGAACESAEKILAETRKAYC
Sbjct: 121 SKLLPEMEVDDNSKREQLLLGMHNMPVSTQIEKLKTRIEMIGAACESAEKILAETRKAYC 180

Query: 181 FGMRQGPAIAPTLDKAQAAKIQEQENLLRAAVNFGEGLRLPGEQRQITPTLPMHLIDVLN 240
           FGMRQGPAIAPTLDKAQAAKIQEQENLLRAAVNFGEGLRLPGEQRQITPTLPMHLIDVLN
Sbjct: 181 FGMRQGPAIAPTLDKAQAAKIQEQENLLRAAVNFGEGLRLPGEQRQITPTLPMHLIDVLN 240

Query: 241 AGDGVQSFNETSGMYAKSTPLTSNNISSQGAVIQ---GKQLL 280
           AGDGVQSFNETSGMYAKSTPLTSNNISSQGAVIQ   G QL+
Sbjct: 241 AGDGVQSFNETSGMYAKSTPLTSNNISSQGAVIQQPPGSQLV 282

[Close]

BLAST of CSPI03G28140 vs. NCBI nr
Match: gi|700203287|gb|KGN58420.1| (hypothetical protein Csa_3G640620 [Cucumis sativus])

HSP 1 Score: 529.3 bits (1362), Expect = 4.5e-147
Identity = 274/274 (100.00%), Postives = 274/274 (100.00%), Query Frame = 1


Query: 1   MATIDGAVQNQPQQQQPLPAQQVVFAERLNQAVQQQLNLEALKNRATTLFKAISRILEDF 60
           MATIDGAVQNQPQQQQPLPAQQVVFAERLNQAVQQQLNLEALKNRATTLFKAISRILEDF
Sbjct: 1   MATIDGAVQNQPQQQQPLPAQQVVFAERLNQAVQQQLNLEALKNRATTLFKAISRILEDF 60

Query: 61  DVYGRTNTTPKWQDILGQYSMVNLELFNIVDEIKKVSKAFVVHPKNVNAENSTILPVMLS 120
           DVYGRTNTTPKWQDILGQYSMVNLELFNIVDEIKKVSKAFVVHPKNVNAENSTILPVMLS
Sbjct: 61  DVYGRTNTTPKWQDILGQYSMVNLELFNIVDEIKKVSKAFVVHPKNVNAENSTILPVMLS 120

Query: 121 SKLLPEMEVDDNSKREQLLLGMHNMPVSTQIEKLKTRIEMIGAACESAEKILAETRKAYC 180
           SKLLPEMEVDDNSKREQLLLGMHNMPVSTQIEKLKTRIEMIGAACESAEKILAETRKAYC
Sbjct: 121 SKLLPEMEVDDNSKREQLLLGMHNMPVSTQIEKLKTRIEMIGAACESAEKILAETRKAYC 180

Query: 181 FGMRQGPAIAPTLDKAQAAKIQEQENLLRAAVNFGEGLRLPGEQRQITPTLPMHLIDVLN 240
           FGMRQGPAIAPTLDKAQAAKIQEQENLLRAAVNFGEGLRLPGEQRQITPTLPMHLIDVLN
Sbjct: 181 FGMRQGPAIAPTLDKAQAAKIQEQENLLRAAVNFGEGLRLPGEQRQITPTLPMHLIDVLN 240

Query: 241 AGDGVQSFNETSGMYAKSTPLTSNNISSQGAVIQ 275
           AGDGVQSFNETSGMYAKSTPLTSNNISSQGAVIQ
Sbjct: 241 AGDGVQSFNETSGMYAKSTPLTSNNISSQGAVIQ 274

[Close]

BLAST of CSPI03G28140 vs. NCBI nr
Match: gi|659130303|ref|XP_008465098.1| (PREDICTED: mediator of RNA polymerase II transcription subunit 8 isoform X2 [Cucumis melo])

HSP 1 Score: 523.5 bits (1347), Expect = 2.5e-145
Identity = 274/281 (97.51%), Postives = 275/281 (97.86%), Query Frame = 1


Query: 1   MATIDGAVQNQPQQQQPLPAQQVVFAERLNQAVQQQLNLEALKNRATTLFKAISRILEDF 60
           MATIDGAVQNQPQQQQPLPAQQVVFAERLNQAVQQQLNLEALKNRATTLFKAISRILEDF
Sbjct: 1   MATIDGAVQNQPQQQQPLPAQQVVFAERLNQAVQQQLNLEALKNRATTLFKAISRILEDF 60

Query: 61  DVYGRTNTTPKWQDILGQYSMVNLELFNIVDEIKKVSKAFVVHPKNVNAENSTILPVMLS 120
           DVYGRTNTTPKWQDILGQYSMVNLELFNIVDEIKKVSKAFVVHPKNVNAENSTILPVMLS
Sbjct: 61  DVYGRTNTTPKWQDILGQYSMVNLELFNIVDEIKKVSKAFVVHPKNVNAENSTILPVMLS 120

Query: 121 SKLLPEMEVDDNSKREQLLLGMHNMPVSTQIEKLKTRIEMIGAACESAEKILAETRKAYC 180
           SKLLPEMEVDDNSKREQLLLGMHNMPVSTQIEKLKTRIEMIGAACESAEKILAETRKAYC
Sbjct: 121 SKLLPEMEVDDNSKREQLLLGMHNMPVSTQIEKLKTRIEMIGAACESAEKILAETRKAYC 180

Query: 181 FGMRQGPAIAPTLDKAQAAKIQEQENLLRAAVNFGEGLRLPGEQRQITPTLPMHLIDVLN 240
           FGMRQGPAI PTLDKAQAAKIQEQENLLRAAVNFGEGLRLPGEQRQIT TLPMHLIDVLN
Sbjct: 181 FGMRQGPAITPTLDKAQAAKIQEQENLLRAAVNFGEGLRLPGEQRQITSTLPMHLIDVLN 240

Query: 241 AGDGVQSFNETSGMYAKSTPLTSNNISSQGAVIQ--GKQLL 280
           AGDGVQSFNETSGMYAKSTPLTS NISSQGAVIQ  G QL+
Sbjct: 241 AGDGVQSFNETSGMYAKSTPLTSTNISSQGAVIQPPGSQLV 281

[Close]

BLAST of CSPI03G28140 vs. NCBI nr
Match: gi|659130301|ref|XP_008465097.1| (PREDICTED: mediator of RNA polymerase II transcription subunit 8 isoform X1 [Cucumis melo])

HSP 1 Score: 523.1 bits (1346), Expect = 3.2e-145
Identity = 274/282 (97.16%), Postives = 275/282 (97.52%), Query Frame = 1


Query: 1   MATIDGAVQNQPQQQQPLPAQQVVFAERLNQAVQQQLNLEALKNRATTLFKAISRILEDF 60
           MATIDGAVQNQPQQQQPLPAQQVVFAERLNQAVQQQLNLEALKNRATTLFKAISRILEDF
Sbjct: 1   MATIDGAVQNQPQQQQPLPAQQVVFAERLNQAVQQQLNLEALKNRATTLFKAISRILEDF 60

Query: 61  DVYGRTNTTPKWQDILGQYSMVNLELFNIVDEIKKVSKAFVVHPKNVNAENSTILPVMLS 120
           DVYGRTNTTPKWQDILGQYSMVNLELFNIVDEIKKVSKAFVVHPKNVNAENSTILPVMLS
Sbjct: 61  DVYGRTNTTPKWQDILGQYSMVNLELFNIVDEIKKVSKAFVVHPKNVNAENSTILPVMLS 120

Query: 121 SKLLPEMEVDDNSKREQLLLGMHNMPVSTQIEKLKTRIEMIGAACESAEKILAETRKAYC 180
           SKLLPEMEVDDNSKREQLLLGMHNMPVSTQIEKLKTRIEMIGAACESAEKILAETRKAYC
Sbjct: 121 SKLLPEMEVDDNSKREQLLLGMHNMPVSTQIEKLKTRIEMIGAACESAEKILAETRKAYC 180

Query: 181 FGMRQGPAIAPTLDKAQAAKIQEQENLLRAAVNFGEGLRLPGEQRQITPTLPMHLIDVLN 240
           FGMRQGPAI PTLDKAQAAKIQEQENLLRAAVNFGEGLRLPGEQRQIT TLPMHLIDVLN
Sbjct: 181 FGMRQGPAITPTLDKAQAAKIQEQENLLRAAVNFGEGLRLPGEQRQITSTLPMHLIDVLN 240

Query: 241 AGDGVQSFNETSGMYAKSTPLTSNNISSQGAVIQ---GKQLL 280
           AGDGVQSFNETSGMYAKSTPLTS NISSQGAVIQ   G QL+
Sbjct: 241 AGDGVQSFNETSGMYAKSTPLTSTNISSQGAVIQQPPGSQLV 282

The following BLAST results are available for this feature:

BLAST of CSPI03G28140 vs. Swiss-Prot


Match Name	E-value	Identity	Description
MED8_ARATH	2.6e-86	60.07	Mediator of RNA polymerase II transcription subunit 8 OS=Arabidopsis thaliana GN...	[more]

BLAST of CSPI03G28140 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L8Z2_CUCSA	3.1e-147	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G640620 PE=4 SV=1	[more]
A0A061GA94_THECC	6.0e-114	77.42	Mediator subunit 8 isoform 1 OS=Theobroma cacao GN=TCM_027824 PE=4 SV=1	[more]
A0A061GGZ8_THECC	6.0e-114	77.42	Mediator subunit 8 isoform 2 OS=Theobroma cacao GN=TCM_027824 PE=4 SV=1	[more]
A0A061G8Z4_THECC	1.7e-113	78.31	Mediator subunit 8 isoform 3 OS=Theobroma cacao GN=TCM_027824 PE=4 SV=1	[more]
V4UNK2_9ROSI	6.6e-113	75.61	Uncharacterized protein OS=Citrus clementina GN=CICLE_v10025265mg PE=4 SV=1	[more]

BLAST of CSPI03G28140 vs. TAIR10


Match Name	E-value	Identity	Description
AT2G03070.1	1.5e-87	60.07	mediator subunit 8	[more]

BLAST of CSPI03G28140 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449453344\|ref\|XP_004144418.1\|	2.0e-147	98.58	PREDICTED: mediator of RNA polymerase II transcription subunit 8 isoform X2 [Cuc...	[more]
gi\|778682360\|ref\|XP_011651696.1\|	2.6e-147	98.23	PREDICTED: mediator of RNA polymerase II transcription subunit 8 isoform X1 [Cuc...	[more]
gi\|700203287\|gb\|KGN58420.1\|	4.5e-147	100.00	hypothetical protein Csa_3G640620 [Cucumis sativus]	[more]
gi\|659130303\|ref\|XP_008465098.1\|	2.5e-145	97.51	PREDICTED: mediator of RNA polymerase II transcription subunit 8 isoform X2 [Cuc...	[more]
gi\|659130301\|ref\|XP_008465097.1\|	3.2e-145	97.16	PREDICTED: mediator of RNA polymerase II transcription subunit 8 isoform X1 [Cuc...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0050832	defense response to fungus
biological_process	GO:0009909	regulation of flower development
cellular_component	GO:0016592	mediator complex
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G28140.1	CSPI03G28140.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR35552	FAMILY NOT NAMED	coord: 1..274 score: 1.5

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CSPI03G28140	Csa3G640620	Cucumber (Chinese Long) v2	cpicuB120
CSPI03G28140	Lsi07G006760	Bottle gourd (USVL1VR-Ls)	cpilsiB232

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CSPI03G28140	Cucurbita pepo (Zucchini)	cpecpiB603
CSPI03G28140	Melon (DHL92) v3.6.1	cpimedB194
CSPI03G28140	Cucumber (Gy14) v2	cgybcpiB110
CSPI03G28140	Cucumber (Chinese Long) v3	cpicucB144
CSPI03G28140	Watermelon (97103) v2	cpiwmbB248
CSPI03G28140	Wax gourd	cpiwgoB276
CSPI03G28140	Cucumber (Gy14) v1	cgycpiB221
CSPI03G28140	Cucurbita maxima (Rimu)	cmacpiB245
CSPI03G28140	Cucurbita moschata (Rifu)	cmocpiB234
CSPI03G28140	Melon (DHL92) v3.5.1	cpimeB200
CSPI03G28140	Watermelon (Charleston Gray)	cpiwcgB262