CSPI03G27970 (gene) Wild cucumber (PI 183967) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CSPI03G27970
Type	gene
Organism	Cucumis sativus (Wild cucumber (PI 183967))
Description	Isoflavone reductase homolog
Location	Chr3 : 25541917 .. 25545539 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTGTGTTTGCATATAACATTGATTTATCTCACATCATTCTCAAATTAAATGAAAATTCCCATGCTTATGTTATGCTTATAACCTTAAAACGTATGCTTAAGGGAAGAATTGAATTGAGAAAGGAAAAAAAAAAAAAAAAGCAAAGAGAATTTATTTGATCTAAAAAGTGGAATAGTTTTGTGCTTTTAAATTAGAATGGAAGCAATTTAAAAGCGAAGCAATGTAAGAGGAAGAGAGGGAGAGAGGAAGAGAAGGAGAGAGGGGAAAAGGGCTATAAAAGGAGAAAGTTGAGTTTGATTAGAAGAGGAGAAAATTAAAGAGAATAGAAAGAGAGGAAAAAAATGAGAGTTCTTGTAGTTGGAGGAACTGGCTACTTAGGGAAGAGGATTGTAAAGGCAAGCCTTTTGGAAGGGCATGAGACATATGTAGTTCAAAGGCCAGAGATCGGACTTGACATTGAGAAGCTTCAATTGCTCCTTTCCTTTAAGAAACAAGGGGCCATCCTCGTTCCGGCTTCTTTCTCCGATTTCCAGAGCCTCGTCGATGCTGTGAAGCGCGTAGATGTCGTCATCTCCGCCCTGTCCGGTGTCCATTTTAGAAGCCATAGCATTCTCCTTCAACTCAAACTTGTTGAAGCCATTCGAGCTGCAGGGAATATCGAGGTTAATTTTCTTTTAATTTTCATTACAAACATTTATTATCTACAAAAGCTTCATCACTCCATTAAAGATTGTAATCTATGAAATAAACTTTATGTGATCTAACAACTTGAGGTTTTAGAGTAATCAAAATGCATGATTTAAGGAAATCCTTGTTGTATTTATGTATTTGTACAGAGATTCTTGCCATCAGAATATGGAATAGATCCAGCAAGGATGTTAAATGCAATTGAACAAGGAAGAGACACATTTGAACAAAAAATGGCAGTAAGAAAGGCAATTGAAGAAGCAAATATTCCATTTACTTATGTGTCGGCCAACTGTTTTGCTGGTTACTTTGCTGCTAACCTCTCTCAAATGCATACTCTTGTTCCTCCAGCCCATCAAGTCACAGTTTATGGTGATGGTAATGTTAAAGGTAACCCCCCCAACCCATCCCCGTACACCGATTATTCATCTTATCTAAATTATTGCCCGCTCCCTCACTTTTTTCTAATTACAACAATTGCCCTTCACTTTGCAATAATTTACTTTTCCAAATAATTGTGGTTTATTCTTTAGAGTTTTTATTTGCGAATGTCTAAATCTTTTTAACAATACTTTTACTTTTACGCCTATATTTTTTTAATTAATATAATTTACGTTATTTTTTGTGTTTTTCAAAAATTGAAAAAAAAAAAAAACAAACTAGTTACCCAAAAATTCATTATACACCTTAGACATTTTTTTTAAATATAGATATTTTGTCGACTTTTCTATTTTTTTTTTTTGACAATTTCTCTTATTTTTTTATATTATTTTATATGATAAAACTTTTAAAAATATCTACGAATATAGCAAAATATCACAATCTATATTAACTATGATTAAACTAAGATAGAGTATTATATCCAAATAGATATAGATAGTAGTTTATCGTGATTCGTTGTACATAAACAATGATACTTTAGTATGTTTTTTAAATATTTTGATTCATTTTATTATACTTAAAAATATAAAAATATTAAACAGAGGAAGCCTCACCATAGACGTTATTGCAAAACAAACTCAACTATTTCAATTTTCATCCTAGATAGTCTTGAATTACTATGAAGCAAAAAACTCTAGGTAATTCAATTTAAAGTCCACTGTTAGTATTTTTTCTTTCTTAATTTGTAAATTGAATATTGGTGAGAGATAATATTCAAACTTTTGAGTTTTTAGTTGAGATATGATAGATATCTTAACTATTAAAAGATATTTCAAGATCGATACTCTTCTCCTATTATTTAGTTATTTAGGGCAAATTAGTTATATTATAATTTCTTAATTCTTAGTTATTTATTGTAAATTAATTCTATTATAATTTCTTAATTCTTATCCATAATGAGAAGGTAAGCAATAGAGAATTATTTCAAACCTTTTCGTTAGCTGTTCTTGTTTACATAAGACTCCACTCTAAACTCTAATTTATATATACTAAACTCAAGTTAGTTGTTAAAATCTAACATCTAAAATTAGAAACAATTATTTTATTTTTAAATGTAATTGTCATGACTTATATACTATAAATCTTTCACTTTCTCCAACATAGTGATGAGAATGTTGTTAATCTGTCTATAACCTTTTATTATGAAGAAAACATGGATTTTTTGACAATGAATTTATACCTTGAATGAAAGTGGTTTAGTTTAAATTTAAATAATGAAATGTAACGTGTAACTTACAGTGGTATACATGGACGAAGATGACATTGCAAGGTACGTCGTGAAGGCAGCAACTGATCCTAGAACATTGAATAAAACAGTGTATATTAGGCCTCCAAAAAACATTCTCTCCCAAAGAGAACTCATTGAGAAATGGGAAAAGCTTTCAGGGAAAGTACTGGAAAAGATAAGTGTTTCTGCTCAAGACTTTCTTGCTAAAATAGAAGGTAAACAAATTTTTTATTTCATTTGGGTTTTTTAGAAAGAAATATTATTGTTAGTTTCTTATTTCTACATTTCAAGAAATAATATAGAGAACAAGAAATAAGACTGAAGAAAAGTTATTTCTACATCTCTCCAATTTTTTATCACTGTAACGCATGAAGAAGAGATTCTCCAATTTGAAAATTTTGAAATAATTCTAGAACTTGAAATGGAAAAAGAATCAAGCCGGAGAAATAAAAGAAAATGTAAAACCAAAATAGTAATACTCGGGCATATCTTGGTTAGCATCCTTTTTGTACCATGGGAGAAATTATGGTCGGATATAATTAAGTGAGCACCGATTAAAACGATTGTTCGACTTAGAATACACTTTAATATACGTTAACAAAAATTATCTAAGATGAGACTTTCTGAATAACGTGTGTATTGAAATGTCATTTCGGTCTTTACACTTTAAAATTTGTTTAATTATGTTGAAGCACATATTAGTATTTTCTTGTAACAAAATTAACTTTGAAAGATTAAGTTTAAGGTTACATTGATGGTACAATTAATAAGAAGAAAATGGTATTGTAATGTATATATTAAATGTAAATTTGATGGTGCAGATCTGGATGAAGCACAGCAGTCTGGAGCTGGACATTTTTATGATATATTCTTTGAAGGTTGTTTGACAAATTTTGAAATAGGAGAAGGAGCTGAAGAAGCTTCAAAGCTTTTTCCAGAAGTGAATTACACTACCATGGATGATTATCTCAAGATCTTTTTATAAGATAAAATAACAATCAAAACGGATATTTGCAAATTGAATAATTAGATTCAAAATAATTAAGTATATAGCAACATTTTAAAAATTTGAATTTATCAATTTTTATCAATGATATAAGTCTATCACTACCGATAGACCATGTTGCAAATATTGGTCTATCACTAATAGGTCTTTGTTATATTTGCAATTTTTAAAAAATGTTGTTATATCTTAATTATTATTTCTAAAACGGTCATCAATTGCAATTTCCCTAATAAAAGTAAATGATAATTTCTAATCTCTTCC

mRNA sequence

ATGAGAGTTCTTGTAGTTGGAGGAACTGGCTACTTAGGGAAGAGGATTGTAAAGGCAAGCCTTTTGGAAGGGCATGAGACATATGTAGTTCAAAGGCCAGAGATCGGACTTGACATTGAGAAGCTTCAATTGCTCCTTTCCTTTAAGAAACAAGGGGCCATCCTCGTTCCGGCTTCTTTCTCCGATTTCCAGAGCCTCGTCGATGCTGTGAAGCGCGTAGATGTCGTCATCTCCGCCCTGTCCGGTGTCCATTTTAGAAGCCATAGCATTCTCCTTCAACTCAAACTTGTTGAAGCCATTCGAGCTGCAGGGAATATCGAGAGATTCTTGCCATCAGAATATGGAATAGATCCAGCAAGGATGTTAAATGCAATTGAACAAGGAAGAGACACATTTGAACAAAAAATGGCAGTAAGAAAGGCAATTGAAGAAGCAAATATTCCATTTACTTATGTGTCGGCCAACTGTTTTGCTGGTTACTTTGCTGCTAACCTCTCTCAAATGCATACTCTTGTTCCTCCAGCCCATCAAGTCACAGTTTATGGTGATGGTAATGTTAAAGTGGTATACATGGACGAAGATGACATTGCAAGGTACGTCGTGAAGGCAGCAACTGATCCTAGAACATTGAATAAAACAGTGTATATTAGGCCTCCAAAAAACATTCTCTCCCAAAGAGAACTCATTGAGAAATGGGAAAAGCTTTCAGGGAAAGTACTGGAAAAGATAAGTGTTTCTGCTCAAGACTTTCTTGCTAAAATAGAAGATCTGGATGAAGCACAGCAGTCTGGAGCTGGACATTTTTATGATATATTCTTTGAAGGTTGTTTGACAAATTTTGAAATAGGAGAAGGAGCTGAAGAAGCTTCAAAGCTTTTTCCAGAAGTGAATTACACTACCATGGATGATTATCTCAAGATCTTTTTATAA

Coding sequence (CDS)

ATGAGAGTTCTTGTAGTTGGAGGAACTGGCTACTTAGGGAAGAGGATTGTAAAGGCAAGCCTTTTGGAAGGGCATGAGACATATGTAGTTCAAAGGCCAGAGATCGGACTTGACATTGAGAAGCTTCAATTGCTCCTTTCCTTTAAGAAACAAGGGGCCATCCTCGTTCCGGCTTCTTTCTCCGATTTCCAGAGCCTCGTCGATGCTGTGAAGCGCGTAGATGTCGTCATCTCCGCCCTGTCCGGTGTCCATTTTAGAAGCCATAGCATTCTCCTTCAACTCAAACTTGTTGAAGCCATTCGAGCTGCAGGGAATATCGAGAGATTCTTGCCATCAGAATATGGAATAGATCCAGCAAGGATGTTAAATGCAATTGAACAAGGAAGAGACACATTTGAACAAAAAATGGCAGTAAGAAAGGCAATTGAAGAAGCAAATATTCCATTTACTTATGTGTCGGCCAACTGTTTTGCTGGTTACTTTGCTGCTAACCTCTCTCAAATGCATACTCTTGTTCCTCCAGCCCATCAAGTCACAGTTTATGGTGATGGTAATGTTAAAGTGGTATACATGGACGAAGATGACATTGCAAGGTACGTCGTGAAGGCAGCAACTGATCCTAGAACATTGAATAAAACAGTGTATATTAGGCCTCCAAAAAACATTCTCTCCCAAAGAGAACTCATTGAGAAATGGGAAAAGCTTTCAGGGAAAGTACTGGAAAAGATAAGTGTTTCTGCTCAAGACTTTCTTGCTAAAATAGAAGATCTGGATGAAGCACAGCAGTCTGGAGCTGGACATTTTTATGATATATTCTTTGAAGGTTGTTTGACAAATTTTGAAATAGGAGAAGGAGCTGAAGAAGCTTCAAAGCTTTTTCCAGAAGTGAATTACACTACCATGGATGATTATCTCAAGATCTTTTTATAA

Homology

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BLAST of CSPI03G27970 vs. Swiss-Prot
Match: IFRH_LUPAL (Isoflavone reductase homolog OS=Lupinus albus PE=2 SV=1)

HSP 1 Score: 465.3 bits (1196), Expect = 5.2e-130
Identity = 226/308 (73.38%), Postives = 267/308 (86.69%), Query Frame = 1


Query: 2   RVLVVGGTGYLGKRIVKASLLEGHETYVVQRPEIGLDIEKLQLLLSFKKQGAILVPASFS 61
           +VLVVGGTGY+G+RIVKASL  GHET+++QRPEIGLDIEKLQ+LLSFKKQGAILV ASFS
Sbjct: 5   KVLVVGGTGYVGRRIVKASLEHGHETFILQRPEIGLDIEKLQILLSFKKQGAILVEASFS 64

Query: 62  DFQSLVDAVKRVDVVISALSGVHFRSHSILLQLKLVEAIRAAGNIERFLPSEYGIDPARM 121
           D +SLVDAVK VDVVI  +SGVHFRSH++L QLKLVEAI+ AGNI+RFLPSE+G+DPA M
Sbjct: 65  DHKSLVDAVKLVDVVICTMSGVHFRSHNLLTQLKLVEAIKDAGNIKRFLPSEFGMDPALM 124

Query: 122 LNAIEQGRDTFEQKMAVRKAIEEANIPFTYVSANCFAGYFAANLSQMHTLVPPAHQVTVY 181
            +A+E GR TF++KM VRKAIEEANIPFTY+SANCFAGYFA NLSQM TL+PP  +V +Y
Sbjct: 125 GHALEPGRVTFDEKMTVRKAIEEANIPFTYISANCFAGYFAGNLSQMKTLLPPRDKVLLY 184

Query: 182 GDGNVKVVYMDEDDIARYVVKAATDPRTLNKTVYIRPPKNILSQRELIEKWEKLSGKVLE 241
           GDGNVK VYMDEDD+A Y +K   DPRTLNKTVY+RPP+NIL+ +ELIEKWE+L GK LE
Sbjct: 185 GDGNVKPVYMDEDDVATYTIKTIDDPRTLNKTVYLRPPENILTHKELIEKWEELIGKQLE 244

Query: 242 KISVSAQDFLAKIEDLDEAQQSGAGHFYDIFFEGCLTNFEIGEGAEEASKLFPEVNYTTM 301
           K S+S +DFL+ ++ LD A Q G GHFY IF+EGCLTNFEIGE  EEAS+L+PEVNYT M
Sbjct: 245 KNSISEKDFLSTLKGLDFASQVGVGHFYHIFYEGCLTNFEIGENGEEASELYPEVNYTRM 304

Query: 302 DDYLKIFL 310
           D YLK+++
Sbjct: 305 DQYLKVYV 312

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BLAST of CSPI03G27970 vs. Swiss-Prot
Match: PILR1_LINPE (Bifunctional pinoresinol-lariciresinol reductase OS=Linum perenne GN=PLR_Lp1 PE=1 SV=1)

HSP 1 Score: 440.3 bits (1131), Expect = 1.8e-122
Identity = 217/309 (70.23%), Postives = 258/309 (83.50%), Query Frame = 1


Query: 3   VLVVGGTGYLGKRIVKASLLEGHETYVVQRPEIGLDIEKLQLLLSFKKQGAILVPASFSD 62
           VLVVGGTGY+GKRIV ASL  GH+TYV++RP  GLDIEKLQLLLSFKK+GA LV ASFSD
Sbjct: 6   VLVVGGTGYIGKRIVSASLYLGHDTYVLKRPGTGLDIEKLQLLLSFKKRGAHLVEASFSD 65

Query: 63  FQSLVDAVKRVDVVISALSGVHFRSHSILLQLKLVEAIRAAGNIERFLPSEYGIDPARML 122
             SLV AV+ VDVVI  +SGVHFRSH+ILLQLKLVEAI+ AGN++RF+PSE+G+DPARM 
Sbjct: 66  HDSLVRAVRLVDVVICTMSGVHFRSHNILLQLKLVEAIKEAGNVKRFIPSEFGMDPARMG 125

Query: 123 NAIEQGRDTFEQKMAVRKAIEEANIPFTYVSANCFAGYFAANLSQMHTLVPPAHQVTVYG 182
            A+E GR+TF+QKM VRKAIEEANIP TY+SANCFAGYF  NLSQ+ TL PP+ +V +YG
Sbjct: 126 QAMEPGRETFDQKMVVRKAIEEANIPHTYISANCFAGYFVGNLSQLGTLTPPSDKVIIYG 185

Query: 183 DGNVKVVYMDEDDIARYVVKAATDPRTLNKTVYIRPPKNILSQRELIEKWEKLSGKVLEK 242
           DGNVKVVY+DEDD+A+Y +KA  D RT+NKTVY+RPP+N++SQREL+  WEKLSG  LEK
Sbjct: 186 DGNVKVVYVDEDDVAKYTIKAIEDDRTVNKTVYLRPPENMMSQRELVAVWEKLSGNQLEK 245

Query: 243 ISVSAQDFLAKIEDLDEAQQSGAGHFYDIFFEGCLTNFEIG--EGAEEASKLFPEVNYTT 302
           I +  QDFLA +E    A+Q+G GHFY IF+EGCLTNFEI    G EEAS+L+PEV YT 
Sbjct: 246 IELPPQDFLALMEGTTVAEQAGIGHFYHIFYEGCLTNFEINAENGEEEASRLYPEVEYTR 305

Query: 303 MDDYLKIFL 310
           + DYLKI+L
Sbjct: 306 VHDYLKIYL 314

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BLAST of CSPI03G27970 vs. Swiss-Prot
Match: PILR1_ARATH (Pinoresinol reductase 1 OS=Arabidopsis thaliana GN=PRR1 PE=1 SV=1)

HSP 1 Score: 428.7 bits (1101), Expect = 5.4e-119
Identity = 210/308 (68.18%), Postives = 254/308 (82.47%), Query Frame = 1


Query: 2   RVLVVGGTGYLGKRIVKASLLEGHETYVVQRPEIGLDIEKLQLLLSFKKQGAILVPASFS 61
           RVLVVG TGY+GKRIV+A L EGHETYV+QRPEIGL+IEK+QL LSFKK GA +V  SFS
Sbjct: 11  RVLVVGATGYIGKRIVRACLAEGHETYVLQRPEIGLEIEKVQLFLSFKKLGARIVEGSFS 70

Query: 62  DFQSLVDAVKRVDVVISALSGVHFRSHSILLQLKLVEAIRAAGNIERFLPSEYGIDPARM 121
           D QSLV AVK VDVV+SA+SGVHFRSH+IL+QLKLVEAI+ AGN++RFLPSE+G+DP RM
Sbjct: 71  DHQSLVSAVKLVDVVVSAMSGVHFRSHNILVQLKLVEAIKEAGNVKRFLPSEFGMDPPRM 130

Query: 122 LNAIEQGRDTFEQKMAVRKAIEEANIPFTYVSANCFAGYFAANLSQMHTLVPPAHQVTVY 181
            +A+  GR+TF+QKM VR+AIE A IP+TYV   CFA YFA NLSQM TL+PP  +V +Y
Sbjct: 131 GHALPPGRETFDQKMEVRQAIEAAGIPYTYVVGACFAAYFAGNLSQMVTLLPPKEKVNIY 190

Query: 182 GDGNVKVVYMDEDDIARYVVKAATDPRTLNKTVYIRPPKNILSQRELIEKWEKLSGKVLE 241
           GDGNVKVV+ DEDDIA+Y  K   DPRTLNKTV IRPP N+L+Q EL++ WEKL+GK LE
Sbjct: 191 GDGNVKVVFADEDDIAKYTAKTLNDPRTLNKTVNIRPPDNVLTQLELVQIWEKLTGKELE 250

Query: 242 KISVSAQDFLAKIEDLDEAQQSGAGHFYDIFFEGCLTNFEIGEGAEEASKLFPEVNYTTM 301
           K +++AQDFLA IE ++   Q+G GHFY IF+EGCLT+ E+GE  EEAS L+P+V Y  M
Sbjct: 251 KTNIAAQDFLANIEQMEIPHQAGIGHFYHIFYEGCLTDHEVGED-EEASSLYPDVKYKRM 310

Query: 302 DDYLKIFL 310
           DDYL++FL
Sbjct: 311 DDYLRMFL 317

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BLAST of CSPI03G27970 vs. Swiss-Prot
Match: PILR2_LINUS (Bifunctional pinoresinol-lariciresinol reductase 2 OS=Linum usitatissimum GN=PLR_Lu2 PE=1 SV=1)

HSP 1 Score: 419.1 bits (1076), Expect = 4.3e-116
Identity = 204/308 (66.23%), Postives = 251/308 (81.49%), Query Frame = 1


Query: 2   RVLVVGGTGYLGKRIVKASLLEGHETYVVQRPEIGLDIEKLQLLLSFKKQGAILVPASFS 61
           +VLV+GGTGYLGKR+V ASL  GHETYV+QRPEIG+DIEK+QLLLSFKK GA LV  SF+
Sbjct: 23  KVLVIGGTGYLGKRLVTASLAAGHETYVLQRPEIGVDIEKIQLLLSFKKAGASLVSGSFN 82

Query: 62  DFQSLVDAVKRVDVVISALSGVHFRSHSILLQLKLVEAIRAAGNIERFLPSEYGIDPARM 121
           D++SLVDAVK VDVVI A+SGVH RSH ILLQLKLV+AI+ AGN++RFLPSE+G DPA M
Sbjct: 83  DYRSLVDAVKLVDVVICAVSGVHIRSHQILLQLKLVDAIKEAGNVKRFLPSEFGTDPATM 142

Query: 122 LNAIEQGRDTFEQKMAVRKAIEEANIPFTYVSANCFAGYFAANLSQMHTLVPPAHQVTVY 181
            NA+E GR TF+ KM VRKAIEEA IPFTY+SANCFAGYF   L Q   ++P   QVT+ 
Sbjct: 143 ENAMEPGRVTFDDKMVVRKAIEEAGIPFTYISANCFAGYFLGGLCQPGFILPSREQVTLL 202

Query: 182 GDGNVKVVYMDEDDIARYVVKAATDPRTLNKTVYIRPPKNILSQRELIEKWEKLSGKVLE 241
           GDGN K VY+DEDDIARY +K   DPRTLNKTVYI+PPKN+LSQRE++  WEK  GK L+
Sbjct: 203 GDGNQKAVYVDEDDIARYTIKMIDDPRTLNKTVYIKPPKNVLSQREVVGIWEKYIGKELK 262

Query: 242 KISVSAQDFLAKIEDLDEAQQSGAGHFYDIFFEGCLTNFEIGEGAEEASKLFPEVNYTTM 301
           K ++S ++FLA +++ D A+Q G  H+Y + +EGCLTNFEIG+ A EA+KL+PEV YTT+
Sbjct: 263 KTTLSVEEFLAMMKEQDYAEQVGLTHYYHVCYEGCLTNFEIGDEAGEATKLYPEVGYTTV 322

Query: 302 DDYLKIFL 310
            +Y+K ++
Sbjct: 323 VEYMKRYV 330

[Close]

BLAST of CSPI03G27970 vs. Swiss-Prot
Match: PILR_LINUS (Bifunctional pinoresinol-lariciresinol reductase OS=Linum usitatissimum PE=2 SV=1)

HSP 1 Score: 411.4 bits (1056), Expect = 8.9e-114
Identity = 204/310 (65.81%), Postives = 248/310 (80.00%), Query Frame = 1


Query: 2   RVLVVGGTGYLGKRIVKASLLEGHETYVVQRPEIGLDIEKLQLLLSFKKQGAILVPASFS 61
           RVLVVGGTGY+GKRIVKAS+  GH+TYV++RPE GLDIEK QLLLSFKKQGA LV ASFS
Sbjct: 5   RVLVVGGTGYIGKRIVKASIEHGHDTYVLKRPETGLDIEKFQLLLSFKKQGAHLVEASFS 64

Query: 62  DFQSLVDAVKRVDVVISALSGVHFRSHSILLQLKLVEAIRAAGNIERFLPSEYGIDPARM 121
           D +SLV AVK VDVVI  +SG H  S S+LLQLKLVEAI+ AGN++RF+PSE+G+DPARM
Sbjct: 65  DHESLVRAVKLVDVVICTVSGAH--SRSLLLQLKLVEAIKEAGNVKRFIPSEFGMDPARM 124

Query: 122 LNAIEQGRDTFEQKMAVRKAIEEANIPFTYVSANCFAGYFAANLSQMHTLVPPAHQVTVY 181
            +A+E GR+TF+ KM VRKAIE+ANIP TY+SANCF GYF  NLSQ+  L PP+ +VT+Y
Sbjct: 125 GDALEPGRETFDLKMVVRKAIEDANIPHTYISANCFGGYFVGNLSQLGPLTPPSDKVTIY 184

Query: 182 GDGNVKVVYMDEDDIARYVVKAATDPRTLNKTVYIRPPKNILSQRELIEKWEKLSGKVLE 241
           GDGNVKVVYMDEDD+A Y +    D RTLNKT+Y RPP+N+++ R+L+E WEKLSG  L+
Sbjct: 185 GDGNVKVVYMDEDDVATYTIMTIEDDRTLNKTMYFRPPENVITHRQLVETWEKLSGNQLQ 244

Query: 242 KISVSAQDFLAKIEDLDEAQQSGAGHFYDIFFEGCLTNFEIGEGAE--EASKLFPEVNYT 301
           K  +S+QDFLA +E  D A+Q   GH Y I++EGCLTNF+I    +  EAS L+PEV YT
Sbjct: 245 KTELSSQDFLALMEGKDVAEQIVIGHLYHIYYEGCLTNFDIDADQDQVEASSLYPEVEYT 304

Query: 302 TMDDYLKIFL 310
            M DYL I+L
Sbjct: 305 RMKDYLMIYL 312

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BLAST of CSPI03G27970 vs. TrEMBL
Match: A0A0A0LE76_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G636990 PE=4 SV=1)

HSP 1 Score: 536.2 bits (1380), Expect = 2.7e-149
Identity = 275/275 (100.00%), Postives = 275/275 (100.00%), Query Frame = 1


Query: 1   MRVLVVGGTGYLGKRIVKASLLEGHETYVVQRPEIGLDIEKLQLLLSFKKQGAILVPASF 60
           MRVLVVGGTGYLGKRIVKASLLEGHETYVVQRPEIGLDIEKLQLLLSFKKQGAILVPASF
Sbjct: 1   MRVLVVGGTGYLGKRIVKASLLEGHETYVVQRPEIGLDIEKLQLLLSFKKQGAILVPASF 60

Query: 61  SDFQSLVDAVKRVDVVISALSGVHFRSHSILLQLKLVEAIRAAGNIERFLPSEYGIDPAR 120
           SDFQSLVDAVKRVDVVISALSGVHFRSHSILLQLKLVEAIRAAGNIERFLPSEYGIDPAR
Sbjct: 61  SDFQSLVDAVKRVDVVISALSGVHFRSHSILLQLKLVEAIRAAGNIERFLPSEYGIDPAR 120

Query: 121 MLNAIEQGRDTFEQKMAVRKAIEEANIPFTYVSANCFAGYFAANLSQMHTLVPPAHQVTV 180
           MLNAIEQGRDTFEQKMAVRKAIEEANIPFTYVSANCFAGYFAANLSQMHTLVPPAHQVTV
Sbjct: 121 MLNAIEQGRDTFEQKMAVRKAIEEANIPFTYVSANCFAGYFAANLSQMHTLVPPAHQVTV 180

Query: 181 YGDGNVKVVYMDEDDIARYVVKAATDPRTLNKTVYIRPPKNILSQRELIEKWEKLSGKVL 240
           YGDGNVKVVYMDEDDIARYVVKAATDPRTLNKTVYIRPPKNILSQRELIEKWEKLSGKVL
Sbjct: 181 YGDGNVKVVYMDEDDIARYVVKAATDPRTLNKTVYIRPPKNILSQRELIEKWEKLSGKVL 240

Query: 241 EKISVSAQDFLAKIEDLDEAQQSGAGHFYDIFFEG 276
           EKISVSAQDFLAKIEDLDEAQQSGAGHFYDIFFEG
Sbjct: 241 EKISVSAQDFLAKIEDLDEAQQSGAGHFYDIFFEG 275

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BLAST of CSPI03G27970 vs. TrEMBL
Match: V7CJ47_PHAVU (Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_002G132400g PE=4 SV=1)

HSP 1 Score: 474.2 bits (1219), Expect = 1.2e-130
Identity = 228/308 (74.03%), Postives = 271/308 (87.99%), Query Frame = 1


Query: 2   RVLVVGGTGYLGKRIVKASLLEGHETYVVQRPEIGLDIEKLQLLLSFKKQGAILVPASFS 61
           +VLVVGGTGY+G+RIVKASL +GHETYV+QRPE+GLDIEKLQ+LLSFKKQGA LV ASFS
Sbjct: 5   KVLVVGGTGYVGRRIVKASLEQGHETYVLQRPELGLDIEKLQMLLSFKKQGAHLVEASFS 64

Query: 62  DFQSLVDAVKRVDVVISALSGVHFRSHSILLQLKLVEAIRAAGNIERFLPSEYGIDPARM 121
           D QSLVDAVK VDVVI  +SGVHFRSHS+L+QL+LVEAI+ AGN++RFLPSE+G+DPA M
Sbjct: 65  DHQSLVDAVKLVDVVICTMSGVHFRSHSLLMQLQLVEAIKVAGNVKRFLPSEFGMDPALM 124

Query: 122 LNAIEQGRDTFEQKMAVRKAIEEANIPFTYVSANCFAGYFAANLSQMHTLVPPAHQVTVY 181
            +A+E GR TF+ KM VRKAIE+ANIPFTY+SANCFAGYF  NLSQM TL+PP  +V +Y
Sbjct: 125 GHALEPGRVTFDDKMTVRKAIEDANIPFTYISANCFAGYFVGNLSQMGTLLPPRDKVILY 184

Query: 182 GDGNVKVVYMDEDDIARYVVKAATDPRTLNKTVYIRPPKNILSQRELIEKWEKLSGKVLE 241
           GDGNVKVVYMDEDD+A Y +KA  DPRTLNKTVY+RPP+NIL+QR+LIEKWEKL+GK LE
Sbjct: 185 GDGNVKVVYMDEDDVATYTIKATDDPRTLNKTVYLRPPENILTQRQLIEKWEKLTGKQLE 244

Query: 242 KISVSAQDFLAKIEDLDEAQQSGAGHFYDIFFEGCLTNFEIGEGAEEASKLFPEVNYTTM 301
           K S++ QDFLA ++ LD A Q G GHFY +F+EGCLTNFEIGEG EEAS L+PEVNYT M
Sbjct: 245 KSSINEQDFLASMKGLDYAGQVGVGHFYHVFYEGCLTNFEIGEGGEEASALYPEVNYTRM 304

Query: 302 DDYLKIFL 310
           D+YL++++
Sbjct: 305 DEYLQVYV 312

[Close]

BLAST of CSPI03G27970 vs. TrEMBL
Match: I1L546_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_09G211500 PE=4 SV=1)

HSP 1 Score: 470.3 bits (1209), Expect = 1.8e-129
Identity = 228/307 (74.27%), Postives = 270/307 (87.95%), Query Frame = 1


Query: 2   RVLVVGGTGYLGKRIVKASLLEGHETYVVQRPEIGLDIEKLQLLLSFKKQGAILVPASFS 61
           +VLVVGGTGY+G+RIVKASL +GHETYV+QRPEIGLDIEK+Q+LLSFKKQGA LV AS S
Sbjct: 5   KVLVVGGTGYVGRRIVKASLEQGHETYVLQRPEIGLDIEKVQMLLSFKKQGAHLVEASVS 64

Query: 62  DFQSLVDAVKRVDVVISALSGVHFRSHSILLQLKLVEAIRAAGNIERFLPSEYGIDPARM 121
           D QSLV+AVK VDVVI  +SGVHFRSH++L+QLKLVEAI+AAGN++RFLPSE+G+DPA M
Sbjct: 65  DHQSLVEAVKLVDVVICTMSGVHFRSHNLLVQLKLVEAIKAAGNVKRFLPSEFGMDPALM 124

Query: 122 LNAIEQGRDTFEQKMAVRKAIEEANIPFTYVSANCFAGYFAANLSQMHTLVPPAHQVTVY 181
            +A+E GR TF++KM VRKAIE+ANIPFTY+SANCFAGYFA NLSQM TL+PP  +V +Y
Sbjct: 125 GHALEPGRVTFDEKMTVRKAIEDANIPFTYISANCFAGYFAGNLSQMGTLLPPRDKVLLY 184

Query: 182 GDGNVKVVYMDEDDIARYVVKAATDPRTLNKTVYIRPPKNILSQRELIEKWEKLSGKVLE 241
           GDGNVKVVYM+EDD+A Y +K   DPRTLNKTVY+RPP+NIL+QR+LIEKWEKL GK LE
Sbjct: 185 GDGNVKVVYMNEDDVAAYTIKTIDDPRTLNKTVYLRPPENILTQRQLIEKWEKLIGKQLE 244

Query: 242 KISVSAQDFLAKIEDLDEAQQSGAGHFYDIFFEGCLTNFEIGEGAEEASKLFPEVNYTTM 301
           K S++ QDFLA I+ LD A Q G GHFY IF+EGCLTNFEIGEG EEAS+L+PEV YT M
Sbjct: 245 KSSINEQDFLASIKGLDYAAQVGVGHFYHIFYEGCLTNFEIGEGGEEASELYPEVKYTRM 304

Query: 302 DDYLKIF 309
           D+YLK++
Sbjct: 305 DEYLKVY 311

[Close]

BLAST of CSPI03G27970 vs. TrEMBL
Match: A0A0L9URK3_PHAAN (Uncharacterized protein OS=Phaseolus angularis GN=LR48_Vigan06g068900 PE=4 SV=1)

HSP 1 Score: 467.6 bits (1202), Expect = 1.2e-128
Identity = 224/308 (72.73%), Postives = 270/308 (87.66%), Query Frame = 1


Query: 2   RVLVVGGTGYLGKRIVKASLLEGHETYVVQRPEIGLDIEKLQLLLSFKKQGAILVPASFS 61
           +VLVVGGTGY+G+RIVKASL +GH+TYV+QRP++GLDI+KLQ+LLSFKKQGA LV ASFS
Sbjct: 5   KVLVVGGTGYVGRRIVKASLEQGHQTYVLQRPDLGLDIDKLQMLLSFKKQGAHLVEASFS 64

Query: 62  DFQSLVDAVKRVDVVISALSGVHFRSHSILLQLKLVEAIRAAGNIERFLPSEYGIDPARM 121
           D QSLVDAVK VDVVI  +SGVHFRSH++LLQL+LVEAI+ AGN++RFLPSE+G+DPA M
Sbjct: 65  DHQSLVDAVKLVDVVICTMSGVHFRSHNLLLQLQLVEAIKVAGNVKRFLPSEFGMDPALM 124

Query: 122 LNAIEQGRDTFEQKMAVRKAIEEANIPFTYVSANCFAGYFAANLSQMHTLVPPAHQVTVY 181
            +A+E GR TF++KM VRKAIE+ANIPFTY+SANCFAGYF +NLSQM TL+PP  +V +Y
Sbjct: 125 GHALEPGRVTFDEKMTVRKAIEDANIPFTYISANCFAGYFVSNLSQMGTLLPPRDKVILY 184

Query: 182 GDGNVKVVYMDEDDIARYVVKAATDPRTLNKTVYIRPPKNILSQRELIEKWEKLSGKVLE 241
           GDGNVK V+MDEDD+A Y +K   DPRTLNKTVY+RPP+NIL+QR+LIEKWEKL GK LE
Sbjct: 185 GDGNVKAVFMDEDDVATYTIKTIDDPRTLNKTVYLRPPENILTQRQLIEKWEKLIGKQLE 244

Query: 242 KISVSAQDFLAKIEDLDEAQQSGAGHFYDIFFEGCLTNFEIGEGAEEASKLFPEVNYTTM 301
           K S++ QDFLA ++ LD A Q G GHFY IF+EGCLTNFEIGEG EEAS L+PEVNYT M
Sbjct: 245 KSSINEQDFLASMKGLDYATQVGVGHFYHIFYEGCLTNFEIGEGGEEASTLYPEVNYTRM 304

Query: 302 DDYLKIFL 310
           D+YLK+++
Sbjct: 305 DEYLKVYV 312

[Close]

BLAST of CSPI03G27970 vs. TrEMBL
Match: L0P1B3_LUPAN (Similar to isoflavone reductase homolog OS=Lupinus angustifolius PE=4 SV=1)

HSP 1 Score: 467.6 bits (1202), Expect = 1.2e-128
Identity = 228/308 (74.03%), Postives = 266/308 (86.36%), Query Frame = 1


Query: 2   RVLVVGGTGYLGKRIVKASLLEGHETYVVQRPEIGLDIEKLQLLLSFKKQGAILVPASFS 61
           +VLVVGGTGY+G+RIVKASL  GHETYV+QRPEIGLDIEKLQ+LLSFKKQGAILV ASFS
Sbjct: 5   KVLVVGGTGYVGRRIVKASLEHGHETYVLQRPEIGLDIEKLQILLSFKKQGAILVEASFS 64

Query: 62  DFQSLVDAVKRVDVVISALSGVHFRSHSILLQLKLVEAIRAAGNIERFLPSEYGIDPARM 121
           D QSLVDAVK VDVVI  +SGVHFRSH++L QLKLVEAI+ A NI+RF PSE+G+DPA M
Sbjct: 65  DHQSLVDAVKLVDVVICTMSGVHFRSHNLLTQLKLVEAIKDAANIKRFYPSEFGMDPALM 124

Query: 122 LNAIEQGRDTFEQKMAVRKAIEEANIPFTYVSANCFAGYFAANLSQMHTLVPPAHQVTVY 181
            +A+E GR TF++KM VRKAIEEANIPFTY+SANCFAGYFA NLSQM TL+PP  +V +Y
Sbjct: 125 GHALEPGRVTFDEKMIVRKAIEEANIPFTYISANCFAGYFAGNLSQMKTLLPPRDKVLLY 184

Query: 182 GDGNVKVVYMDEDDIARYVVKAATDPRTLNKTVYIRPPKNILSQRELIEKWEKLSGKVLE 241
           GDGNVK VYMDEDD+A Y +K   DPRTLNKTVY+RPP+NIL+QRELI+KWE+L GK LE
Sbjct: 185 GDGNVKAVYMDEDDVATYTIKTIDDPRTLNKTVYLRPPENILTQRELIQKWEELIGKQLE 244

Query: 242 KISVSAQDFLAKIEDLDEAQQSGAGHFYDIFFEGCLTNFEIGEGAEEASKLFPEVNYTTM 301
           K ++S QDFL+ ++ LD A Q G GHFY IF+EGCLTNFEIGE  EEAS+L+PEVNYT M
Sbjct: 245 KSTISEQDFLSTLKGLDFASQVGVGHFYHIFYEGCLTNFEIGENGEEASELYPEVNYTRM 304

Query: 302 DDYLKIFL 310
           D YLK+++
Sbjct: 305 DQYLKVYV 312

[Close]

BLAST of CSPI03G27970 vs. TAIR10
Match: AT1G32100.1 (AT1G32100.1 pinoresinol reductase 1)

HSP 1 Score: 428.7 bits (1101), Expect = 3.0e-120
Identity = 210/308 (68.18%), Postives = 254/308 (82.47%), Query Frame = 1


Query: 2   RVLVVGGTGYLGKRIVKASLLEGHETYVVQRPEIGLDIEKLQLLLSFKKQGAILVPASFS 61
           RVLVVG TGY+GKRIV+A L EGHETYV+QRPEIGL+IEK+QL LSFKK GA +V  SFS
Sbjct: 11  RVLVVGATGYIGKRIVRACLAEGHETYVLQRPEIGLEIEKVQLFLSFKKLGARIVEGSFS 70

Query: 62  DFQSLVDAVKRVDVVISALSGVHFRSHSILLQLKLVEAIRAAGNIERFLPSEYGIDPARM 121
           D QSLV AVK VDVV+SA+SGVHFRSH+IL+QLKLVEAI+ AGN++RFLPSE+G+DP RM
Sbjct: 71  DHQSLVSAVKLVDVVVSAMSGVHFRSHNILVQLKLVEAIKEAGNVKRFLPSEFGMDPPRM 130

Query: 122 LNAIEQGRDTFEQKMAVRKAIEEANIPFTYVSANCFAGYFAANLSQMHTLVPPAHQVTVY 181
            +A+  GR+TF+QKM VR+AIE A IP+TYV   CFA YFA NLSQM TL+PP  +V +Y
Sbjct: 131 GHALPPGRETFDQKMEVRQAIEAAGIPYTYVVGACFAAYFAGNLSQMVTLLPPKEKVNIY 190

Query: 182 GDGNVKVVYMDEDDIARYVVKAATDPRTLNKTVYIRPPKNILSQRELIEKWEKLSGKVLE 241
           GDGNVKVV+ DEDDIA+Y  K   DPRTLNKTV IRPP N+L+Q EL++ WEKL+GK LE
Sbjct: 191 GDGNVKVVFADEDDIAKYTAKTLNDPRTLNKTVNIRPPDNVLTQLELVQIWEKLTGKELE 250

Query: 242 KISVSAQDFLAKIEDLDEAQQSGAGHFYDIFFEGCLTNFEIGEGAEEASKLFPEVNYTTM 301
           K +++AQDFLA IE ++   Q+G GHFY IF+EGCLT+ E+GE  EEAS L+P+V Y  M
Sbjct: 251 KTNIAAQDFLANIEQMEIPHQAGIGHFYHIFYEGCLTDHEVGED-EEASSLYPDVKYKRM 310

Query: 302 DDYLKIFL 310
           DDYL++FL
Sbjct: 311 DDYLRMFL 317

[Close]

BLAST of CSPI03G27970 vs. TAIR10
Match: AT4G13660.1 (AT4G13660.1 pinoresinol reductase 2)

HSP 1 Score: 404.4 bits (1038), Expect = 6.1e-113
Identity = 199/308 (64.61%), Postives = 248/308 (80.52%), Query Frame = 1


Query: 2   RVLVVGGTGYLGKRIVKASLLEGHETYVVQRPEIGLDIEKLQLLLSFKKQGAILVPASFS 61
           RVLVVGGTG LG+RIV A L EGHETYV+QRPEIG+DIEK+QLLLSFK+ GA LV  SFS
Sbjct: 11  RVLVVGGTGSLGRRIVSACLAEGHETYVLQRPEIGVDIEKVQLLLSFKRLGAHLVEGSFS 70

Query: 62  DFQSLVDAVKRVDVVISALSGVHFRSHSILLQLKLVEAIRAAGNIERFLPSEYGIDPARM 121
           D QSLV AVK+VDVV+SA+SGVHFR+H+I +QLKLV AI+ AGN++RFLPSE+G+DP+RM
Sbjct: 71  DHQSLVSAVKQVDVVVSAMSGVHFRTHNIPVQLKLVAAIKEAGNVKRFLPSEFGMDPSRM 130

Query: 122 LNAIEQGRDTFEQKMAVRKAIEEANIPFTYVSANCFAGYFAANLSQMHTLVPPAHQVTVY 181
            +A+  G +TF+QKM +R AI+ A I  TY+   CFA YF  NLSQM TL PP ++V +Y
Sbjct: 131 GHAMPPGSETFDQKMEIRNAIKAAGISHTYLVGACFAAYFGGNLSQMGTLFPPKNKVDIY 190

Query: 182 GDGNVKVVYMDEDDIARYVVKAATDPRTLNKTVYIRPPKNILSQRELIEKWEKLSGKVLE 241
           GDGNVKVV++DEDD+A+Y  K   DPRTLNKTVY+RP  NIL+Q EL++ WEKL+ K LE
Sbjct: 191 GDGNVKVVFVDEDDMAKYTAKTLNDPRTLNKTVYVRPTDNILTQMELVQIWEKLTEKELE 250

Query: 242 KISVSAQDFLAKIEDLDEAQQSGAGHFYDIFFEGCLTNFEIGEGAEEASKLFPEVNYTTM 301
           K  VS  DFLA IED + + Q+G GHFY I++EGCLT+ E+G+  EEA+KL+P+V Y  M
Sbjct: 251 KTYVSGNDFLADIEDKEISHQAGLGHFYHIYYEGCLTDHEVGDD-EEATKLYPDVKYKRM 310

Query: 302 DDYLKIFL 310
           D+YLKIF+
Sbjct: 311 DEYLKIFV 317

[Close]

BLAST of CSPI03G27970 vs. TAIR10
Match: AT1G75290.1 (AT1G75290.1 NAD(P)-binding Rossmann-fold superfamily protein)

HSP 1 Score: 234.6 bits (597), Expect = 8.4e-62
Identity = 128/310 (41.29%), Postives = 188/310 (60.65%), Query Frame = 1


Query: 2   RVLVVGGTGYLGKRIVKASLLEGHETYVVQRPEIGLDIEKLQLLLSFKKQGAILVPASFS 61
           ++LV+GGTG++GK I++AS+  GH T  + R     D  K + + +FK  G  L+    +
Sbjct: 7   KILVIGGTGHIGKLIIEASVKAGHSTLALVREASLSDPNKGKTVQNFKDFGVTLLHGDLN 66

Query: 62  DFQSLVDAVKRVDVVISALSGVHFRSHSILLQLKLVEAIRAAGNIERFLPSEYGIDPARM 121
           D +SLV A+K+ DVVIS +      S  IL Q K++ AI+ AGN++RFLPSE+G+D  + 
Sbjct: 67  DHESLVKAIKQADVVISTVG-----SMQILDQTKIISAIKEAGNVKRFLPSEFGMDVDKS 126

Query: 122 LNAIEQGRDTFEQKMAVRKAIEEANIPFTYVSANCFAGYFAANLSQMHTLV--PPAHQVT 181
            +A+E  +  F +K+  R+ IE   IP+TY+  N FAGY+   L Q+   +  PP  +V 
Sbjct: 127 -SAVEPAKSAFGRKLQTRRDIEAEGIPYTYLVTNYFAGYYLPTLVQLEPGLTSPPRDKVK 186

Query: 182 VYGDGNVKVVYMDEDDIARYVVKAATDPRTLNKTVYIRPPKNILSQRELIEKWEKLSGKV 241
           ++GDGNVK V   E+DIA Y +KA  DPRTLNKT+YI PP N LS  E++  WEK  GK 
Sbjct: 187 IFGDGNVKAVINKEEDIAAYTIKAVDDPRTLNKTLYINPPNNTLSMNEIVTLWEKKIGKS 246

Query: 242 LEKISVSAQDFLAKIEDLDEAQQSGAGHFYDIFFEGCLTNFEIGEG-AEEASKLFPEVNY 301
           +EKI +S +     I++            + +F +G  TNF I      EAS+L+P++ Y
Sbjct: 247 VEKIYMSEEQIFKSIQESPVPFNVLLSINHAVFVKGDQTNFTIEPSFGFEASELYPDIKY 306

Query: 302 TTMDDYLKIF 309
           T++D+YL  F
Sbjct: 307 TSIDEYLSYF 310

[Close]

BLAST of CSPI03G27970 vs. TAIR10
Match: AT1G75300.1 (AT1G75300.1 NmrA-like negative transcriptional regulator family protein)

HSP 1 Score: 229.9 bits (585), Expect = 2.1e-60
Identity = 134/324 (41.36%), Postives = 192/324 (59.26%), Query Frame = 1


Query: 2   RVLVVGGTGYLGKRIVKASLLEGHETYVVQRPEIGLDIEKLQLLLSFKKQGAILVPASFS 61
           ++LV+GGTGY+G+ IV+ S   G+ T+ + R     D  K + + SFK  G  ++    +
Sbjct: 7   KILVIGGTGYMGEFIVEGSAKAGNPTFALVREASLSDPVKSKTIQSFKDLGVTILHGDLN 66

Query: 62  DFQSLVDAVKRVDVVISALSGVHFRSHSILLQLKLVEAIRAAGNIERFLPSEYGIDPARM 121
           D +SLV A+K+VDVVIS +         I  Q K++ AI+ AGN++RFLP+E+GID  R 
Sbjct: 67  DHESLVKAIKQVDVVISTIG-----HKQIFDQTKIISAIKEAGNVKRFLPAEFGIDVERT 126

Query: 122 LNAIEQGRDTFEQKMAVRKAIEEANIPFTYVSANCFAGYFAANLSQM------HTL---- 181
            +A+E  +  F  K+ +R+AIE   IP+TYV +NC AG++   L Q       HT     
Sbjct: 127 -SAVEPAKSLFAGKVQIRRAIEAEGIPYTYVVSNCSAGFYLRTLLQFESGLISHTRDKAI 186

Query: 182 ------VPPAHQVTVYGDGNVKVVYMDEDDIARYVVKAATDPRTLNKTVYIRPPKNILSQ 241
                 VPP  +VT+ GDGN KVV   E+D+A Y++KA  D RTLNKT+YI PP NILS 
Sbjct: 187 IFGDKNVPPRDKVTILGDGNAKVVINKEEDVAAYMIKAVDDLRTLNKTLYISPPNNILSM 246

Query: 242 RELIEKWEKLSGKVLEKISVSAQDFLAKIEDLDEAQQSGAGHFYDIFFEGCLTNFEIGEG 301
            E++  WEK  GK LEK  +S +  L  I+   +  +S     + +F +G  T+F I   
Sbjct: 247 NEMVTLWEKKIGKSLEKTHISEEQILKSIQVPIDVFKS---INHAVFVKGDQTSFTIEPW 306

Query: 302 -AEEASKLFPEVNYTTMDDYLKIF 309
             EEAS L+P+V YT++D+YL  F
Sbjct: 307 FGEEASVLYPDVKYTSIDEYLSQF 321

[Close]

BLAST of CSPI03G27970 vs. TAIR10
Match: AT1G75280.1 (AT1G75280.1 NmrA-like negative transcriptional regulator family protein)

HSP 1 Score: 228.4 bits (581), Expect = 6.0e-60
Identity = 128/310 (41.29%), Postives = 181/310 (58.39%), Query Frame = 1


Query: 2   RVLVVGGTGYLGKRIVKASLLEGHETYVVQRPEIGLDIEKLQLLLSFKKQGAILVPASFS 61
           ++LV+GGTGY+GK +V+AS   GH T+ + R     D  K + + SFK  G  ++    +
Sbjct: 7   KILVIGGTGYIGKFLVEASAKAGHSTFALVREATLSDPVKGKTVQSFKDLGVTILHGDLN 66

Query: 62  DFQSLVDAVKRVDVVISALSGVHFRSHSILLQLKLVEAIRAAGNIERFLPSEYGIDPARM 121
           D +SLV A+K+VDVVIS +      S  IL Q K++ AI+ AGN++RFLPSE+G+D  R 
Sbjct: 67  DHESLVKAIKQVDVVISTVG-----SMQILDQTKIISAIKEAGNVKRFLPSEFGVDVDRT 126

Query: 122 LNAIEQGRDTFEQKMAVRKAIEEANIPFTYVSANCFAGYFAANLSQMHTLV--PPAHQVT 181
            +A+E  +  F  K+ +R+ IE   IP+TY    CF GY+   L Q    +  PP  +VT
Sbjct: 127 -SAVEPAKSAFAGKIQIRRTIEAEGIPYTYAVTGCFGGYYLPTLVQFEPGLTSPPRDKVT 186

Query: 182 VYGDGNVKVVYMDEDDIARYVVKAATDPRTLNKTVYIRPPKNILSQRELIEKWEKLSGKV 241
           + GDGN K V   E+DIA Y +KA  DPRTLNK +YI+P  N LS  E++  WEK  GK 
Sbjct: 187 ILGDGNAKAVINKEEDIAAYTIKAVDDPRTLNKILYIKPSNNTLSMNEIVTLWEKKIGKS 246

Query: 242 LEKISVSAQDFLAKIEDLDEAQQSGAGHFYDIFFEGCLTNFEIGEG-AEEASKLFPEVNY 301
           LEK  +  +  L  I++            + +F  G  TN  I      EAS+L+P+V Y
Sbjct: 247 LEKTHLPEEQLLKSIQESPIPINVVLSINHAVFVNGD-TNISIEPSFGVEASELYPDVKY 306

Query: 302 TTMDDYLKIF 309
           T++D+YL  F
Sbjct: 307 TSVDEYLSYF 309

[Close]

BLAST of CSPI03G27970 vs. NCBI nr
Match: gi|449453322|ref|XP_004144407.1| (PREDICTED: isoflavone reductase homolog [Cucumis sativus])

HSP 1 Score: 607.1 bits (1564), Expect = 1.8e-170
Identity = 309/309 (100.00%), Postives = 309/309 (100.00%), Query Frame = 1


Query: 1   MRVLVVGGTGYLGKRIVKASLLEGHETYVVQRPEIGLDIEKLQLLLSFKKQGAILVPASF 60
           MRVLVVGGTGYLGKRIVKASLLEGHETYVVQRPEIGLDIEKLQLLLSFKKQGAILVPASF
Sbjct: 1   MRVLVVGGTGYLGKRIVKASLLEGHETYVVQRPEIGLDIEKLQLLLSFKKQGAILVPASF 60

Query: 61  SDFQSLVDAVKRVDVVISALSGVHFRSHSILLQLKLVEAIRAAGNIERFLPSEYGIDPAR 120
           SDFQSLVDAVKRVDVVISALSGVHFRSHSILLQLKLVEAIRAAGNIERFLPSEYGIDPAR
Sbjct: 61  SDFQSLVDAVKRVDVVISALSGVHFRSHSILLQLKLVEAIRAAGNIERFLPSEYGIDPAR 120

Query: 121 MLNAIEQGRDTFEQKMAVRKAIEEANIPFTYVSANCFAGYFAANLSQMHTLVPPAHQVTV 180
           MLNAIEQGRDTFEQKMAVRKAIEEANIPFTYVSANCFAGYFAANLSQMHTLVPPAHQVTV
Sbjct: 121 MLNAIEQGRDTFEQKMAVRKAIEEANIPFTYVSANCFAGYFAANLSQMHTLVPPAHQVTV 180

Query: 181 YGDGNVKVVYMDEDDIARYVVKAATDPRTLNKTVYIRPPKNILSQRELIEKWEKLSGKVL 240
           YGDGNVKVVYMDEDDIARYVVKAATDPRTLNKTVYIRPPKNILSQRELIEKWEKLSGKVL
Sbjct: 181 YGDGNVKVVYMDEDDIARYVVKAATDPRTLNKTVYIRPPKNILSQRELIEKWEKLSGKVL 240

Query: 241 EKISVSAQDFLAKIEDLDEAQQSGAGHFYDIFFEGCLTNFEIGEGAEEASKLFPEVNYTT 300
           EKISVSAQDFLAKIEDLDEAQQSGAGHFYDIFFEGCLTNFEIGEGAEEASKLFPEVNYTT
Sbjct: 241 EKISVSAQDFLAKIEDLDEAQQSGAGHFYDIFFEGCLTNFEIGEGAEEASKLFPEVNYTT 300

Query: 301 MDDYLKIFL 310
           MDDYLKIFL
Sbjct: 301 MDDYLKIFL 309

[Close]

BLAST of CSPI03G27970 vs. NCBI nr
Match: gi|659120967|ref|XP_008460437.1| (PREDICTED: isoflavone reductase homolog [Cucumis melo])

HSP 1 Score: 570.1 bits (1468), Expect = 2.4e-159
Identity = 287/309 (92.88%), Postives = 300/309 (97.09%), Query Frame = 1


Query: 1   MRVLVVGGTGYLGKRIVKASLLEGHETYVVQRPEIGLDIEKLQLLLSFKKQGAILVPASF 60
           MRVLVVGGTGYLGKRIVKASLLEGHETYVVQRPEIGLDIEKLQLLLSFK+QGAILVPASF
Sbjct: 1   MRVLVVGGTGYLGKRIVKASLLEGHETYVVQRPEIGLDIEKLQLLLSFKRQGAILVPASF 60

Query: 61  SDFQSLVDAVKRVDVVISALSGVHFRSHSILLQLKLVEAIRAAGNIERFLPSEYGIDPAR 120
           SD QSLVDAVKRVDVVISALSGVHFRSHSILLQLKLVEAI+AAGNI+RFLPSEYGIDP+R
Sbjct: 61  SDIQSLVDAVKRVDVVISALSGVHFRSHSILLQLKLVEAIQAAGNIKRFLPSEYGIDPSR 120

Query: 121 MLNAIEQGRDTFEQKMAVRKAIEEANIPFTYVSANCFAGYFAANLSQMHTLVPPAHQVTV 180
           M NAIEQGRDTF+QKM +RKAIEEANIPFTYVSANCFAGYFAANLSQM TLVPP HQVTV
Sbjct: 121 MGNAIEQGRDTFDQKMVIRKAIEEANIPFTYVSANCFAGYFAANLSQMETLVPPTHQVTV 180

Query: 181 YGDGNVKVVYMDEDDIARYVVKAATDPRTLNKTVYIRPPKNILSQRELIEKWEKLSGKVL 240
           YGDGNVKV+Y DEDDIARYVVKAATDPRTLNKTVYIRPP+NILSQRELI+ WEKLSGKVL
Sbjct: 181 YGDGNVKVIYTDEDDIARYVVKAATDPRTLNKTVYIRPPQNILSQRELIQIWEKLSGKVL 240

Query: 241 EKISVSAQDFLAKIEDLDEAQQSGAGHFYDIFFEGCLTNFEIGEGAEEASKLFPEVNYTT 300
           EKISVSAQDFLA I+DLDEAQQ+GAGHFY+IFF+GCLTNFEIGE AEEASKLFPEVNYTT
Sbjct: 241 EKISVSAQDFLANIKDLDEAQQAGAGHFYEIFFKGCLTNFEIGEAAEEASKLFPEVNYTT 300

Query: 301 MDDYLKIFL 310
           MDDYLKIFL
Sbjct: 301 MDDYLKIFL 309

[Close]

BLAST of CSPI03G27970 vs. NCBI nr
Match: gi|700203268|gb|KGN58401.1| (hypothetical protein Csa_3G636990 [Cucumis sativus])

HSP 1 Score: 536.2 bits (1380), Expect = 3.8e-149
Identity = 275/275 (100.00%), Postives = 275/275 (100.00%), Query Frame = 1


Query: 1   MRVLVVGGTGYLGKRIVKASLLEGHETYVVQRPEIGLDIEKLQLLLSFKKQGAILVPASF 60
           MRVLVVGGTGYLGKRIVKASLLEGHETYVVQRPEIGLDIEKLQLLLSFKKQGAILVPASF
Sbjct: 1   MRVLVVGGTGYLGKRIVKASLLEGHETYVVQRPEIGLDIEKLQLLLSFKKQGAILVPASF 60

Query: 61  SDFQSLVDAVKRVDVVISALSGVHFRSHSILLQLKLVEAIRAAGNIERFLPSEYGIDPAR 120
           SDFQSLVDAVKRVDVVISALSGVHFRSHSILLQLKLVEAIRAAGNIERFLPSEYGIDPAR
Sbjct: 61  SDFQSLVDAVKRVDVVISALSGVHFRSHSILLQLKLVEAIRAAGNIERFLPSEYGIDPAR 120

Query: 121 MLNAIEQGRDTFEQKMAVRKAIEEANIPFTYVSANCFAGYFAANLSQMHTLVPPAHQVTV 180
           MLNAIEQGRDTFEQKMAVRKAIEEANIPFTYVSANCFAGYFAANLSQMHTLVPPAHQVTV
Sbjct: 121 MLNAIEQGRDTFEQKMAVRKAIEEANIPFTYVSANCFAGYFAANLSQMHTLVPPAHQVTV 180

Query: 181 YGDGNVKVVYMDEDDIARYVVKAATDPRTLNKTVYIRPPKNILSQRELIEKWEKLSGKVL 240
           YGDGNVKVVYMDEDDIARYVVKAATDPRTLNKTVYIRPPKNILSQRELIEKWEKLSGKVL
Sbjct: 181 YGDGNVKVVYMDEDDIARYVVKAATDPRTLNKTVYIRPPKNILSQRELIEKWEKLSGKVL 240

Query: 241 EKISVSAQDFLAKIEDLDEAQQSGAGHFYDIFFEG 276
           EKISVSAQDFLAKIEDLDEAQQSGAGHFYDIFFEG
Sbjct: 241 EKISVSAQDFLAKIEDLDEAQQSGAGHFYDIFFEG 275

[Close]

BLAST of CSPI03G27970 vs. NCBI nr
Match: gi|593790318|ref|XP_007158198.1| (hypothetical protein PHAVU_002G132400g [Phaseolus vulgaris])

HSP 1 Score: 474.2 bits (1219), Expect = 1.8e-130
Identity = 228/308 (74.03%), Postives = 271/308 (87.99%), Query Frame = 1


Query: 2   RVLVVGGTGYLGKRIVKASLLEGHETYVVQRPEIGLDIEKLQLLLSFKKQGAILVPASFS 61
           +VLVVGGTGY+G+RIVKASL +GHETYV+QRPE+GLDIEKLQ+LLSFKKQGA LV ASFS
Sbjct: 5   KVLVVGGTGYVGRRIVKASLEQGHETYVLQRPELGLDIEKLQMLLSFKKQGAHLVEASFS 64

Query: 62  DFQSLVDAVKRVDVVISALSGVHFRSHSILLQLKLVEAIRAAGNIERFLPSEYGIDPARM 121
           D QSLVDAVK VDVVI  +SGVHFRSHS+L+QL+LVEAI+ AGN++RFLPSE+G+DPA M
Sbjct: 65  DHQSLVDAVKLVDVVICTMSGVHFRSHSLLMQLQLVEAIKVAGNVKRFLPSEFGMDPALM 124

Query: 122 LNAIEQGRDTFEQKMAVRKAIEEANIPFTYVSANCFAGYFAANLSQMHTLVPPAHQVTVY 181
            +A+E GR TF+ KM VRKAIE+ANIPFTY+SANCFAGYF  NLSQM TL+PP  +V +Y
Sbjct: 125 GHALEPGRVTFDDKMTVRKAIEDANIPFTYISANCFAGYFVGNLSQMGTLLPPRDKVILY 184

Query: 182 GDGNVKVVYMDEDDIARYVVKAATDPRTLNKTVYIRPPKNILSQRELIEKWEKLSGKVLE 241
           GDGNVKVVYMDEDD+A Y +KA  DPRTLNKTVY+RPP+NIL+QR+LIEKWEKL+GK LE
Sbjct: 185 GDGNVKVVYMDEDDVATYTIKATDDPRTLNKTVYLRPPENILTQRQLIEKWEKLTGKQLE 244

Query: 242 KISVSAQDFLAKIEDLDEAQQSGAGHFYDIFFEGCLTNFEIGEGAEEASKLFPEVNYTTM 301
           K S++ QDFLA ++ LD A Q G GHFY +F+EGCLTNFEIGEG EEAS L+PEVNYT M
Sbjct: 245 KSSINEQDFLASMKGLDYAGQVGVGHFYHVFYEGCLTNFEIGEGGEEASALYPEVNYTRM 304

Query: 302 DDYLKIFL 310
           D+YL++++
Sbjct: 305 DEYLQVYV 312

[Close]

BLAST of CSPI03G27970 vs. NCBI nr
Match: gi|356529761|ref|XP_003533456.1| (PREDICTED: isoflavone reductase homolog [Glycine max])

HSP 1 Score: 470.3 bits (1209), Expect = 2.6e-129
Identity = 228/307 (74.27%), Postives = 270/307 (87.95%), Query Frame = 1


Query: 2   RVLVVGGTGYLGKRIVKASLLEGHETYVVQRPEIGLDIEKLQLLLSFKKQGAILVPASFS 61
           +VLVVGGTGY+G+RIVKASL +GHETYV+QRPEIGLDIEK+Q+LLSFKKQGA LV AS S
Sbjct: 5   KVLVVGGTGYVGRRIVKASLEQGHETYVLQRPEIGLDIEKVQMLLSFKKQGAHLVEASVS 64

Query: 62  DFQSLVDAVKRVDVVISALSGVHFRSHSILLQLKLVEAIRAAGNIERFLPSEYGIDPARM 121
           D QSLV+AVK VDVVI  +SGVHFRSH++L+QLKLVEAI+AAGN++RFLPSE+G+DPA M
Sbjct: 65  DHQSLVEAVKLVDVVICTMSGVHFRSHNLLVQLKLVEAIKAAGNVKRFLPSEFGMDPALM 124

Query: 122 LNAIEQGRDTFEQKMAVRKAIEEANIPFTYVSANCFAGYFAANLSQMHTLVPPAHQVTVY 181
            +A+E GR TF++KM VRKAIE+ANIPFTY+SANCFAGYFA NLSQM TL+PP  +V +Y
Sbjct: 125 GHALEPGRVTFDEKMTVRKAIEDANIPFTYISANCFAGYFAGNLSQMGTLLPPRDKVLLY 184

Query: 182 GDGNVKVVYMDEDDIARYVVKAATDPRTLNKTVYIRPPKNILSQRELIEKWEKLSGKVLE 241
           GDGNVKVVYM+EDD+A Y +K   DPRTLNKTVY+RPP+NIL+QR+LIEKWEKL GK LE
Sbjct: 185 GDGNVKVVYMNEDDVAAYTIKTIDDPRTLNKTVYLRPPENILTQRQLIEKWEKLIGKQLE 244

Query: 242 KISVSAQDFLAKIEDLDEAQQSGAGHFYDIFFEGCLTNFEIGEGAEEASKLFPEVNYTTM 301
           K S++ QDFLA I+ LD A Q G GHFY IF+EGCLTNFEIGEG EEAS+L+PEV YT M
Sbjct: 245 KSSINEQDFLASIKGLDYAAQVGVGHFYHIFYEGCLTNFEIGEGGEEASELYPEVKYTRM 304

Query: 302 DDYLKIF 309
           D+YLK++
Sbjct: 305 DEYLKVY 311

The following BLAST results are available for this feature:

BLAST of CSPI03G27970 vs. Swiss-Prot


Match Name	E-value	Identity	Description
IFRH_LUPAL	5.2e-130	73.38	Isoflavone reductase homolog OS=Lupinus albus PE=2 SV=1	[more]
PILR1_LINPE	1.8e-122	70.23	Bifunctional pinoresinol-lariciresinol reductase OS=Linum perenne GN=PLR_Lp1 PE=...	[more]
PILR1_ARATH	5.4e-119	68.18	Pinoresinol reductase 1 OS=Arabidopsis thaliana GN=PRR1 PE=1 SV=1	[more]
PILR2_LINUS	4.3e-116	66.23	Bifunctional pinoresinol-lariciresinol reductase 2 OS=Linum usitatissimum GN=PLR...	[more]
PILR_LINUS	8.9e-114	65.81	Bifunctional pinoresinol-lariciresinol reductase OS=Linum usitatissimum PE=2 SV=...	[more]

BLAST of CSPI03G27970 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LE76_CUCSA	2.7e-149	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G636990 PE=4 SV=1	[more]
V7CJ47_PHAVU	1.2e-130	74.03	Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_002G132400g PE=4 SV=1	[more]
I1L546_SOYBN	1.8e-129	74.27	Uncharacterized protein OS=Glycine max GN=GLYMA_09G211500 PE=4 SV=1	[more]
A0A0L9URK3_PHAAN	1.2e-128	72.73	Uncharacterized protein OS=Phaseolus angularis GN=LR48_Vigan06g068900 PE=4 SV=1	[more]
L0P1B3_LUPAN	1.2e-128	74.03	Similar to isoflavone reductase homolog OS=Lupinus angustifolius PE=4 SV=1	[more]

BLAST of CSPI03G27970 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G32100.1	3.0e-120	68.18	pinoresinol reductase 1	[more]
AT4G13660.1	6.1e-113	64.61	pinoresinol reductase 2	[more]
AT1G75290.1	8.4e-62	41.29	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT1G75300.1	2.1e-60	41.36	NmrA-like negative transcriptional regulator family protein	[more]
AT1G75280.1	6.0e-60	41.29	NmrA-like negative transcriptional regulator family protein	[more]

BLAST of CSPI03G27970 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449453322\|ref\|XP_004144407.1\|	1.8e-170	100.00	PREDICTED: isoflavone reductase homolog [Cucumis sativus]	[more]
gi\|659120967\|ref\|XP_008460437.1\|	2.4e-159	92.88	PREDICTED: isoflavone reductase homolog [Cucumis melo]	[more]
gi\|700203268\|gb\|KGN58401.1\|	3.8e-149	100.00	hypothetical protein Csa_3G636990 [Cucumis sativus]	[more]
gi\|593790318\|ref\|XP_007158198.1\|	1.8e-130	74.03	hypothetical protein PHAVU_002G132400g [Phaseolus vulgaris]	[more]
gi\|356529761\|ref\|XP_003533456.1\|	2.6e-129	74.27	PREDICTED: isoflavone reductase homolog [Glycine max]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR008030	NmrA-like
IPR016040	NAD(P)-bd_dom

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055114	oxidation-reduction process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0016491	oxidoreductase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G27970.1	CSPI03G27970.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008030	NmrA-like domain	PFAM	PF05368	NmrA	coord: 3..239 score: 2.9
IPR016040	NAD(P)-binding domain	GENE3D	G3DSA:3.40.50.720		coord: 1..109 score: 4.4
IPR016040	NAD(P)-binding domain	unknown	SSF51735	NAD(P)-binding Rossmann-fold domains	coord: 2..254 score: 4.94
None	No IPR available	GENE3D	G3DSA:3.90.25.10		coord: 154..309 score: 9.1
None	No IPR available	PANTHER	PTHR10366	NAD DEPENDENT EPIMERASE/DEHYDRATASE	coord: 2..309 score: 4.7E
None	No IPR available	PANTHER	PTHR10366:SF397	PINORESINOL REDUCTASE 1-RELATED	coord: 2..309 score: 4.7E

Synteny

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CSPI03G27970	Cucurbita maxima (Rimu)	cmacpiB516
CSPI03G27970	Cucurbita maxima (Rimu)	cmacpiB606
CSPI03G27970	Cucurbita moschata (Rifu)	cmocpiB506
CSPI03G27970	Cucurbita moschata (Rifu)	cmocpiB595
CSPI03G27970	Cucumber (Chinese Long) v2	cpicuB120
CSPI03G27970	Melon (DHL92) v3.5.1	cpimeB200
CSPI03G27970	Watermelon (Charleston Gray)	cpiwcgB267
CSPI03G27970	Watermelon (97103) v1	cpiwmB220
CSPI03G27970	Cucurbita pepo (Zucchini)	cpecpiB242
CSPI03G27970	Cucurbita pepo (Zucchini)	cpecpiB718
CSPI03G27970	Bottle gourd (USVL1VR-Ls)	cpilsiB148
CSPI03G27970	Melon (DHL92) v3.6.1	cpimedB194
CSPI03G27970	Cucumber (Gy14) v2	cgybcpiB110
CSPI03G27970	Silver-seed gourd	carcpiB0092
CSPI03G27970	Silver-seed gourd	carcpiB0816
CSPI03G27970	Cucumber (Chinese Long) v3	cpicucB144
CSPI03G27970	Watermelon (97103) v2	cpiwmbB253
CSPI03G27970	Wax gourd	cpiwgoB309
CSPI03G27970	Cucumber (Gy14) v1	cgycpiB221