CSPI03G25100 (gene) Wild cucumber (PI 183967)

NameCSPI03G25100
Typegene
OrganismCucumis sativus (Wild cucumber (PI 183967))
Descriptionalpha/beta-Hydrolases superfamily protein
LocationChr3 : 22322202 .. 22323602 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GGTTTTTCTTAAGAAAAAACAACAAAATGAAAATTAAAAAACAAACCCAATAAAGTTACGTTCGTGAATTTACAATTTTGCCCTCCCCACCTACCACACTTTCCCCATCTTAACATACATTACAACCACCACACACCTTCGTCCCTCACCACTCACAAACTTACAAGCGGCGAAAATGCCAACGGAATCATCACCGCCACCAGAGCCACCACTCAACTTCTGGGGCGATCAACCGGAGGAAGAATTTTATGCTTCACAAGGCGTCCGCAACACCAAATCCTTCTTTGAAACCTCCCATGGCAAGCTCTTCACACAAAGCTTCATCCCTCTTGACTTCCCCGATCTCAAAGGCACCGTCTACATGACTCACGGCTACGGCTCCGACACCGGCTGGATGTTCCAAAAAATTTGCCTCTCCTACGCTTCCTGGGGCTACGCCGTCTTCGCCGCCGACCTCCTCGGCCACGGACGCTCCGATGGCCTCCGCTGTTACCTCGGCGACATGGACAAAATCGCCGCCGCATCCCTCTCCTTCTTCCTCCACACCCGCCGTAGCGAGCCCTACCGCCACCTTCCAGCGTTCCTCTTCGGCGAATCCATGGGCTGCGCCGCCACCATGCTCATGTACTTGCAGTCCGATCCAGACACCTGGACCGGCCTCATCTTTTCAGCTCCCCTCTTCGTCATACCAGAAAATATGAAACCGAGTAAGCTCCGACTCTTCCTATACGGCCTTCTCTTCGGCGTCGCAGACACTTGGGCCGCCATGCCGGACAATAAAATGGTCGGAAAGGCGATTAAGGATCCGGAGAAATTGAAGATAATAGCAGCAAATCCTAGAAGGTACACGGGACCGCCTAGGGTTGGGACGATGAGGGAATTGGTTAGGGTAACGCAGTATATTAGGGATAATTTTTCTAGGGTTACAGCGCCGTTTCTAACGGTACACGGTACGGCGGACGGAGTGACGTGTCCATCGTCGTCGGAATTACTGTATGAGAAGGCGACGAGCGTGGATAAGACGTTGAAGCTATACGATGGGATGTACCATTCGTTGATTCAAGGAGAGCCTGATGAGAATGTAGAGATTGTGCTGAGGGATATGAGGGAGTGGATCGATGAGAGAGCCTTGAGATATGGACCGAAGAAATAGAAATCCTTGAAAAGGATAAAAGAAGATGGAAGTGTGAACGGCGGAAATGGAGGTGTGTGAATGGCAGAAATGGTAAATGTGGTGTGTTTTATGGGTCAAATATTTCTCTATTTCAATTTGTTTTAATTGACTTTTTCTCTTCTTCCTATAATAATTAAATAGACCTTCATTGCTAATATAAGCTGTGTAGTGTGTGTATTACTATTATTTATTACCAGCTCTCTCTCTTCTTCTCATCTTTCCCAGAA

mRNA sequence

ATGCCAACGGAATCATCACCGCCACCAGAGCCACCACTCAACTTCTGGGGCGATCAACCGGAGGAAGAATTTTATGCTTCACAAGGCGTCCGCAACACCAAATCCTTCTTTGAAACCTCCCATGGCAAGCTCTTCACACAAAGCTTCATCCCTCTTGACTTCCCCGATCTCAAAGGCACCGTCTACATGACTCACGGCTACGGCTCCGACACCGGCTGGATGTTCCAAAAAATTTGCCTCTCCTACGCTTCCTGGGGCTACGCCGTCTTCGCCGCCGACCTCCTCGGCCACGGACGCTCCGATGGCCTCCGCTGTTACCTCGGCGACATGGACAAAATCGCCGCCGCATCCCTCTCCTTCTTCCTCCACACCCGCCGTAGCGAGCCCTACCGCCACCTTCCAGCGTTCCTCTTCGGCGAATCCATGGGCTGCGCCGCCACCATGCTCATGTACTTGCAGTCCGATCCAGACACCTGGACCGGCCTCATCTTTTCAGCTCCCCTCTTCGTCATACCAGAAAATATGAAACCGAGTAAGCTCCGACTCTTCCTATACGGCCTTCTCTTCGGCGTCGCAGACACTTGGGCCGCCATGCCGGACAATAAAATGGTCGGAAAGGCGATTAAGGATCCGGAGAAATTGAAGATAATAGCAGCAAATCCTAGAAGGTACACGGGACCGCCTAGGGTTGGGACGATGAGGGAATTGGTTAGGGTAACGCAGTATATTAGGGATAATTTTTCTAGGGTTACAGCGCCGTTTCTAACGGTACACGGTACGGCGGACGGAGTGACGTGTCCATCGTCGTCGGAATTACTGTATGAGAAGGCGACGAGCGTGGATAAGACGTTGAAGCTATACGATGGGATGTACCATTCGTTGATTCAAGGAGAGCCTGATGAGAATGTAGAGATTGTGCTGAGGGATATGAGGGAGTGGATCGATGAGAGAGCCTTGAGATATGGACCGAAGAAATAG

Coding sequence (CDS)

ATGCCAACGGAATCATCACCGCCACCAGAGCCACCACTCAACTTCTGGGGCGATCAACCGGAGGAAGAATTTTATGCTTCACAAGGCGTCCGCAACACCAAATCCTTCTTTGAAACCTCCCATGGCAAGCTCTTCACACAAAGCTTCATCCCTCTTGACTTCCCCGATCTCAAAGGCACCGTCTACATGACTCACGGCTACGGCTCCGACACCGGCTGGATGTTCCAAAAAATTTGCCTCTCCTACGCTTCCTGGGGCTACGCCGTCTTCGCCGCCGACCTCCTCGGCCACGGACGCTCCGATGGCCTCCGCTGTTACCTCGGCGACATGGACAAAATCGCCGCCGCATCCCTCTCCTTCTTCCTCCACACCCGCCGTAGCGAGCCCTACCGCCACCTTCCAGCGTTCCTCTTCGGCGAATCCATGGGCTGCGCCGCCACCATGCTCATGTACTTGCAGTCCGATCCAGACACCTGGACCGGCCTCATCTTTTCAGCTCCCCTCTTCGTCATACCAGAAAATATGAAACCGAGTAAGCTCCGACTCTTCCTATACGGCCTTCTCTTCGGCGTCGCAGACACTTGGGCCGCCATGCCGGACAATAAAATGGTCGGAAAGGCGATTAAGGATCCGGAGAAATTGAAGATAATAGCAGCAAATCCTAGAAGGTACACGGGACCGCCTAGGGTTGGGACGATGAGGGAATTGGTTAGGGTAACGCAGTATATTAGGGATAATTTTTCTAGGGTTACAGCGCCGTTTCTAACGGTACACGGTACGGCGGACGGAGTGACGTGTCCATCGTCGTCGGAATTACTGTATGAGAAGGCGACGAGCGTGGATAAGACGTTGAAGCTATACGATGGGATGTACCATTCGTTGATTCAAGGAGAGCCTGATGAGAATGTAGAGATTGTGCTGAGGGATATGAGGGAGTGGATCGATGAGAGAGCCTTGAGATATGGACCGAAGAAATAG
BLAST of CSPI03G25100 vs. Swiss-Prot
Match: CSE_ARATH (Caffeoylshikimate esterase OS=Arabidopsis thaliana GN=CSE PE=1 SV=1)

HSP 1 Score: 535.4 bits (1378), Expect = 4.3e-151
Identity = 244/316 (77.22%), Postives = 283/316 (89.56%), Query Frame = 1

Query: 9   PEPPLNFWGDQPEEEFYASQGVRNTKSFFETSHGKLFTQSFIPLDFPDLKGTVYMTHGYG 68
           P PP NFWG  PEEE+Y SQGVRN+KS+FET +GKLFTQSF+PLD  ++KGTVYM+HGYG
Sbjct: 16  PPPPPNFWGTMPEEEYYTSQGVRNSKSYFETPNGKLFTQSFLPLD-GEIKGTVYMSHGYG 75

Query: 69  SDTGWMFQKICLSYASWGYAVFAADLLGHGRSDGLRCYLGDMDKIAAASLSFFLHTRRSE 128
           SDT WMFQKIC+S++SWGYAVFAADLLGHGRSDG+RCY+GDM+K+AA SL+FF H R S+
Sbjct: 76  SDTSWMFQKICMSFSSWGYAVFAADLLGHGRSDGIRCYMGDMEKVAATSLAFFKHVRCSD 135

Query: 129 PYRHLPAFLFGESMGCAATMLMYLQSDPDTWTGLIFSAPLFVIPENMKPSKLRLFLYGLL 188
           PY+ LPAFLFGESMG   T+LMY QS+P+TWTGL+FSAPLFVIPE+MKPSK  LF YGLL
Sbjct: 136 PYKDLPAFLFGESMGGLVTLLMYFQSEPETWTGLMFSAPLFVIPEDMKPSKAHLFAYGLL 195

Query: 189 FGVADTWAAMPDNKMVGKAIKDPEKLKIIAANPRRYTGPPRVGTMRELVRVTQYIRDNFS 248
           FG+ADTWAAMPDNKMVGKAIKDPEKLKIIA+NP+RYTG PRVGTMREL+R TQY+++NF 
Sbjct: 196 FGLADTWAAMPDNKMVGKAIKDPEKLKIIASNPQRYTGKPRVGTMRELLRKTQYVQENFG 255

Query: 249 RVTAPFLTVHGTADGVTCPSSSELLYEKATSVDKTLKLYDGMYHSLIQGEPDENVEIVLR 308
           +VT P  T HGTADGVTCP+SS+LLYEKA+S DKTLK+Y+GMYHSLIQGEPDEN EIVL+
Sbjct: 256 KVTIPVFTAHGTADGVTCPTSSKLLYEKASSADKTLKIYEGMYHSLIQGEPDENAEIVLK 315

Query: 309 DMREWIDERALRYGPK 325
           DMREWIDE+  +YG K
Sbjct: 316 DMREWIDEKVKKYGSK 330

BLAST of CSPI03G25100 vs. Swiss-Prot
Match: MGLL_MOUSE (Monoglyceride lipase OS=Mus musculus GN=Mgll PE=1 SV=1)

HSP 1 Score: 94.4 bits (233), Expect = 2.5e-18
Identity = 73/280 (26.07%), Postives = 130/280 (46.43%), Query Frame = 1

Query: 44  LFTQSFIPLDFPDLKGTVYMTHGYGSDTGWMFQKICLSYASWGYAVFAADLLGHGRSDGL 103
           LF + + P   P  K  ++++HG G   G  + ++          VFA D +GHG+S+G 
Sbjct: 30  LFCRYWKPSGTP--KALIFVSHGAGEHCG-RYDELAHMLKGLDMLVFAHDHVGHGQSEGE 89

Query: 104 RCYLGDMDKIAAASLSFFLHTRRSEPYRHLPAFLFGESMGCAATMLMYLQSDPDTWTGLI 163
           R  + D        L      ++  P   +P FL G SMG A ++L+  +  P  ++G++
Sbjct: 90  RMVVSDFQVFVRDVLQHVDTIQKDYP--DVPIFLLGHSMGGAISILVAAER-PTYFSGMV 149

Query: 164 FSAPLFVIPENMKPSKLRLFLYGLL-FGVADTWAAMPDNKMVGKAIKDPEKLKIIAANPR 223
             +PL V+      S L++    LL F + +      D+ ++ +   +  ++ +  ++P 
Sbjct: 150 LISPL-VLANPESASTLKVLAAKLLNFVLPNMTLGRIDSSVLSR---NKSEVDLYNSDPL 209

Query: 224 RYTGPPRVGTMRELVRVTQYIRDNFSRVTAPFLTVHGTADGVTCPSSSELLYEKATSVDK 283
                 +V    +L+     +     R+T PFL + G+AD +     + LL E + S DK
Sbjct: 210 VCRAGLKVCFGIQLLNAVARVERAMPRLTLPFLLLQGSADRLCDSKGAYLLMESSRSQDK 269

Query: 284 TLKLYDGMYHSLIQGEPDENVEIVLRDMREWIDERALRYG 323
           TLK+Y+G YH ++  E  E    VL ++  W+  R    G
Sbjct: 270 TLKMYEGAYH-VLHRELPEVTNSVLHEVNSWVSHRIAAAG 298

BLAST of CSPI03G25100 vs. Swiss-Prot
Match: MGLL_RAT (Monoglyceride lipase OS=Rattus norvegicus GN=Mgll PE=1 SV=1)

HSP 1 Score: 93.2 bits (230), Expect = 5.6e-18
Identity = 75/276 (27.17%), Postives = 135/276 (48.91%), Query Frame = 1

Query: 44  LFTQSFIPLDFPDLKGTVYMTHGYGSDTGWMFQKICLSYASWGYAVFAADLLGHGRSDGL 103
           LF + + P   P  K  ++++HG G   G  + ++          VFA D +GHG+S+G 
Sbjct: 30  LFCRYWKPSGTP--KALIFVSHGAGEHCG-RYDELAQMLKRLDMLVFAHDHVGHGQSEGE 89

Query: 104 RCYLGDMDKIAAASLSFFLHTRRSEPYRHLPAFLFGESMGCAATMLMYLQSDPDTWTGLI 163
           R  + D  ++    L   ++T + + Y  +P FL G SMG A ++L   +  P  ++G+I
Sbjct: 90  RMVVSDF-QVFVRDLLQHVNTVQKD-YPEVPVFLLGHSMGGAISILAAAER-PTHFSGMI 149

Query: 164 FSAPLFVI-PENMKPSKLRLFLYGLL-FGVADTWAAMPDNKMVGKAIKDPEKLKIIAANP 223
             +PL +  PE+   S L++    LL F + +      D+ ++ +   +  ++ +  ++P
Sbjct: 150 LISPLILANPESA--STLKVLAAKLLNFVLPNISLGRIDSSVLSR---NKSEVDLYNSDP 209

Query: 224 RRYTGPPRVGTMRELVRVTQYIRDNFSRVTAPFLTVHGTADGVTCPSSSELLYEKATSVD 283
                  +V    +L+     +     R+T PFL + G+AD +     + LL E + S D
Sbjct: 210 LICHAGVKVCFGIQLLNAVSRVERAMPRLTLPFLLLQGSADRLCDSKGAYLLMESSPSQD 269

Query: 284 KTLKLYDGMYHSLIQGEPDENVEIVLRDMREWIDER 318
           KTLK+Y+G YH ++  E  E    VL ++  W+  R
Sbjct: 270 KTLKMYEGAYH-VLHKELPEVTNSVLHEINTWVSHR 293

BLAST of CSPI03G25100 vs. Swiss-Prot
Match: Y9086_DICDI (Uncharacterized abhydrolase domain-containing protein DDB_G0269086 OS=Dictyostelium discoideum GN=DDB_G0269086 PE=1 SV=2)

HSP 1 Score: 89.0 bits (219), Expect = 1.1e-16
Identity = 73/266 (27.44%), Postives = 125/266 (46.99%), Query Frame = 1

Query: 60  TVYMTHGYGSDTGWMFQKICLSYASWGYAVFAADLLGHGRSDGLRCYLGDMDKIAAASLS 119
           TV M HG G  +G  ++ +   +A  G  V A D  GHG S G+R +   +++ +   + 
Sbjct: 30  TVTMIHGLGEHSG-RYEHVFSRFAEQGIKVNAFDQRGHGISSGVRGHSPSLEQ-SLKDIQ 89

Query: 120 FFLHTRRSEPYRHLPAFLFGESMGCAATMLMYLQSDPDTWTGLIFSAPLFVIPENMKPSK 179
               T  ++    +P F++G S G    +   L+       G I ++PL  I   +K S 
Sbjct: 90  LIASTAETD----VPHFIYGHSFGGCLALHYNLKKKDHHPAGCIVTSPL--IKPAIKVSG 149

Query: 180 LRLFLYGLLFGVADTWAAMPDNKMVGKAIKDPEKLKIIAANPRRYTGPPRV------GTM 239
           ++L +  LL G+  +W        +  +I DP  +   +A    Y     V      G  
Sbjct: 150 VKLSMGNLLGGLMPSWT-------ISNSI-DPTLISKDSAVVNEYKQDKLVHNKISLGMA 209

Query: 240 RELVRVTQYIRDNFSRVTAPFLTVHGTADGVTCPSSSELLYEKA-TSVDKTLKLYDGMYH 299
           + +++ ++ + D   +   P L VH   D +TCP +S+  Y++  ++VDKTLKL++ MYH
Sbjct: 210 KWMLQRSEQLIDLAPQFDTPLLLVHANDDKITCPKASQQFYDRVPSTVDKTLKLWENMYH 269

Query: 300 SL-IQGEPDENVEIVLRDMREWIDER 318
            +  +   +E V  +L    EWI ER
Sbjct: 270 EVHNEFAKEEFVTYIL----EWIKER 275

BLAST of CSPI03G25100 vs. Swiss-Prot
Match: MGLL_SCHPO (Putative monoglyceride lipase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=SPCC5E4.05c PE=1 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 4.5e-07
Identity = 57/198 (28.79%), Postives = 85/198 (42.93%), Query Frame = 1

Query: 133 LPAFLFGESMGCAATMLMYLQ-SDPDTWTGLIFSAPLFVIPENMKPSKLRLFLYGLLFGV 192
           LP FL+G SMG    +   +  +      G+I  AP+     + +P+ L      L+  V
Sbjct: 91  LPLFLWGHSMGGGLALRYGISGTHRHKLAGVIAQAPMLRCHPDTEPNFLLRKALTLVSKV 150

Query: 193 ADTWAAMPDNKMVGKAIKDPEKLKIIAANPRRYTGPPRVGTMRELVRVTQYIRDNFSRVT 252
              +  + D+ +  + I   E +       +R    P V ++  L    Q   D  +R T
Sbjct: 151 HPNF--LFDSDVQSQHITRDEAVN------QRLQDDPLVSSVGSL----QVFSDMLNRGT 210

Query: 253 A----------PFLTVHGTADGVTCPSSSELLYEKATSVDKTLKLYDGMYHSL-IQGEPD 312
                      P L  HGT D VTC  SS+  YE A + DKT + Y G YHSL I+ +P+
Sbjct: 211 KTIELAPQFFLPLLITHGTDDNVTCSDSSKEFYENAGTKDKTYQSYPGFYHSLHIEKKPE 270

Query: 313 ENVEIVLRDMREWIDERA 319
             V   L  +  WI E +
Sbjct: 271 --VYEYLDKVAAWIYEHS 274

BLAST of CSPI03G25100 vs. TrEMBL
Match: A0A0A0LBT8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G539670 PE=4 SV=1)

HSP 1 Score: 677.9 bits (1748), Expect = 6.0e-192
Identity = 324/325 (99.69%), Postives = 325/325 (100.00%), Query Frame = 1

Query: 1   MPTESSPPPEPPLNFWGDQPEEEFYASQGVRNTKSFFETSHGKLFTQSFIPLDFPDLKGT 60
           MPTESSPPPEPPLNFWGDQPEEEFYASQGVRNTKSFFETSHGKLFTQSFIPLDFPDLKGT
Sbjct: 1   MPTESSPPPEPPLNFWGDQPEEEFYASQGVRNTKSFFETSHGKLFTQSFIPLDFPDLKGT 60

Query: 61  VYMTHGYGSDTGWMFQKICLSYASWGYAVFAADLLGHGRSDGLRCYLGDMDKIAAASLSF 120
           VYMTHGYGSDTGWMFQKICLSYASWGYAVFAADLLGHGRSDGLRCYLGDMDKIAAASLSF
Sbjct: 61  VYMTHGYGSDTGWMFQKICLSYASWGYAVFAADLLGHGRSDGLRCYLGDMDKIAAASLSF 120

Query: 121 FLHTRRSEPYRHLPAFLFGESMGCAATMLMYLQSDPDTWTGLIFSAPLFVIPENMKPSKL 180
           FLHTRRSEPYRHLPAFLFGESMGCAATMLMYLQSDPDTWTGLIFSAPLFVIPENMKPSKL
Sbjct: 121 FLHTRRSEPYRHLPAFLFGESMGCAATMLMYLQSDPDTWTGLIFSAPLFVIPENMKPSKL 180

Query: 181 RLFLYGLLFGVADTWAAMPDNKMVGKAIKDPEKLKIIAANPRRYTGPPRVGTMRELVRVT 240
           RLFLYGLLFGVADTWAAMPDNKMVGKAIKDP+KLKIIAANPRRYTGPPRVGTMRELVRVT
Sbjct: 181 RLFLYGLLFGVADTWAAMPDNKMVGKAIKDPQKLKIIAANPRRYTGPPRVGTMRELVRVT 240

Query: 241 QYIRDNFSRVTAPFLTVHGTADGVTCPSSSELLYEKATSVDKTLKLYDGMYHSLIQGEPD 300
           QYIRDNFSRVTAPFLTVHGTADGVTCPSSSELLYEKATSVDKTLKLYDGMYHSLIQGEPD
Sbjct: 241 QYIRDNFSRVTAPFLTVHGTADGVTCPSSSELLYEKATSVDKTLKLYDGMYHSLIQGEPD 300

Query: 301 ENVEIVLRDMREWIDERALRYGPKK 326
           ENVEIVLRDMREWIDERALRYGPKK
Sbjct: 301 ENVEIVLRDMREWIDERALRYGPKK 325

BLAST of CSPI03G25100 vs. TrEMBL
Match: A0A142EHU3_ZIZJJ (Caffeoylshikimate esterase OS=Ziziphus jujuba PE=2 SV=1)

HSP 1 Score: 581.3 bits (1497), Expect = 7.6e-163
Identity = 269/324 (83.02%), Postives = 302/324 (93.21%), Query Frame = 1

Query: 1   MPTESSPPPEPPLNFWGDQPEEEFYASQGVRNTKSFFETSHGKLFTQSFIPLDFPDLKGT 60
           M +E+ PP  PP NFWG+ PEEE+Y SQGVRNTKS+F+T HGK+FTQSF+PLD P++KGT
Sbjct: 1   MQSETLPPETPP-NFWGNMPEEEYYTSQGVRNTKSYFDTPHGKIFTQSFLPLDTPNVKGT 60

Query: 61  VYMTHGYGSDTGWMFQKICLSYASWGYAVFAADLLGHGRSDGLRCYLGDMDKIAAASLSF 120
           VYM+HGYGSDTGW+FQKIC+S+++WGYAVFAADLLGHGRSDGLRCYLGD++K+AAASLS+
Sbjct: 61  VYMSHGYGSDTGWLFQKICISFSTWGYAVFAADLLGHGRSDGLRCYLGDLEKVAAASLSY 120

Query: 121 FLHTRRSEPYRHLPAFLFGESMGCAATMLMYLQSDPDTWTGLIFSAPLFVIPENMKPSKL 180
           F+H RRSEPY+ LPAFLFGESMG AATMLMY QSDPDTWTGLIFSAPLFV+PENMKPSK+
Sbjct: 121 FVHVRRSEPYKDLPAFLFGESMGGAATMLMYFQSDPDTWTGLIFSAPLFVMPENMKPSKV 180

Query: 181 RLFLYGLLFGVADTWAAMPDNKMVGKAIKDPEKLKIIAANPRRYTGPPRVGTMRELVRVT 240
           RLFLYG+LFG+ADTWAAMPDNKMVGKAIKDPEKLKIIA+NPRRYTG PRVGTMREL RV 
Sbjct: 181 RLFLYGMLFGIADTWAAMPDNKMVGKAIKDPEKLKIIASNPRRYTGQPRVGTMRELARVC 240

Query: 241 QYIRDNFSRVTAPFLTVHGTADGVTCPSSSELLYEKATSVDKTLKLYDGMYHSLIQGEPD 300
           QYI+DNFS+VTAPFLTVHGTADGVTCP+SS+LLYEKA+S DK+LKLYDGMYHSLIQGEPD
Sbjct: 241 QYIQDNFSKVTAPFLTVHGTADGVTCPTSSQLLYEKASSADKSLKLYDGMYHSLIQGEPD 300

Query: 301 ENVEIVLRDMREWIDERALRYGPK 325
           EN  IVLRDMREWIDER  RYG K
Sbjct: 301 ENANIVLRDMREWIDERVERYGSK 323

BLAST of CSPI03G25100 vs. TrEMBL
Match: M5WQY3_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa008845mg PE=4 SV=1)

HSP 1 Score: 572.0 bits (1473), Expect = 4.6e-160
Identity = 267/315 (84.76%), Postives = 291/315 (92.38%), Query Frame = 1

Query: 10  EPPLNFWGDQPEEEFYASQGVRNTKSFFETSHGKLFTQSFIPLDFPDLKGTVYMTHGYGS 69
           E P NFWGD PEE++Y SQGVRNTKSFF+T +G++FTQSF+PLD   +KGTV+MTHGYGS
Sbjct: 4   ETPPNFWGDMPEEDYYTSQGVRNTKSFFQTPNGQIFTQSFLPLD-QTVKGTVFMTHGYGS 63

Query: 70  DTGWMFQKICLSYASWGYAVFAADLLGHGRSDGLRCYLGDMDKIAAASLSFFLHTRRSEP 129
           DTGW+FQKIC+ YA+WGYAVFAADLLGHGRSDGLRCYLGDM+K+AA SLSFFLH RR+E 
Sbjct: 64  DTGWLFQKICMHYATWGYAVFAADLLGHGRSDGLRCYLGDMEKVAATSLSFFLHVRRNEL 123

Query: 130 YRHLPAFLFGESMGCAATMLMYLQSDPDTWTGLIFSAPLFVIPENMKPSKLRLFLYGLLF 189
           Y HLPAFLFGESMG AATMLMY QS PDTWTGLIFSAPLFVIPENMKPSK+ LFLYGLLF
Sbjct: 124 YLHLPAFLFGESMGGAATMLMYFQSPPDTWTGLIFSAPLFVIPENMKPSKVHLFLYGLLF 183

Query: 190 GVADTWAAMPDNKMVGKAIKDPEKLKIIAANPRRYTGPPRVGTMRELVRVTQYIRDNFSR 249
           G+ADTWAAMPDNKMVGKAIKDP KL+IIA+NPRRYTGPPRVGTMRE+ RV QYI+DNFSR
Sbjct: 184 GIADTWAAMPDNKMVGKAIKDPAKLRIIASNPRRYTGPPRVGTMREIARVCQYIQDNFSR 243

Query: 250 VTAPFLTVHGTADGVTCPSSSELLYEKATSVDKTLKLYDGMYHSLIQGEPDENVEIVLRD 309
           VT PFLTVHGTADGVTCPSSS+LLYEKA+SVDKTLK+YDGMYHSLIQGEPDEN EIVLRD
Sbjct: 244 VTTPFLTVHGTADGVTCPSSSKLLYEKASSVDKTLKIYDGMYHSLIQGEPDENAEIVLRD 303

Query: 310 MREWIDERALRYGPK 325
           MREWIDER  RYGPK
Sbjct: 304 MREWIDERVERYGPK 317

BLAST of CSPI03G25100 vs. TrEMBL
Match: W9RZF8_9ROSA (Uncharacterized protein OS=Morus notabilis GN=L484_003175 PE=4 SV=1)

HSP 1 Score: 561.6 bits (1446), Expect = 6.2e-157
Identity = 260/321 (81.00%), Postives = 294/321 (91.59%), Query Frame = 1

Query: 4   ESSPPPEPPLNFWGDQPEEEFYASQGVRNTKSFFETSHGKLFTQSFIPLDFPDLKGTVYM 63
           E++ PP    NFWGD PEEE+Y SQGVRNTKS+F+T HGKLFTQSF+PLD P +KG+VYM
Sbjct: 6   ETTSPP----NFWGDMPEEEYYTSQGVRNTKSYFQTPHGKLFTQSFLPLDVPKIKGSVYM 65

Query: 64  THGYGSDTGWMFQKICLSYASWGYAVFAADLLGHGRSDGLRCYLGDMDKIAAASLSFFLH 123
           +HGYGSDTGW+FQKIC+++ +WGYAVFAADLLGHGRSDGL  Y+GD+DK+AAASLSFF+H
Sbjct: 66  SHGYGSDTGWLFQKICINFTNWGYAVFAADLLGHGRSDGLHGYVGDIDKVAAASLSFFVH 125

Query: 124 TRRSEPYRHLPAFLFGESMGCAATMLMYLQSDPDTWTGLIFSAPLFVIPENMKPSKLRLF 183
            RRS+PY HLPAFLFGESMG AATM+MY QS+PDTWTGLIFSAPLFV+PE+MKPSK+RLF
Sbjct: 126 VRRSDPYNHLPAFLFGESMGGAATMMMYFQSEPDTWTGLIFSAPLFVMPEDMKPSKVRLF 185

Query: 184 LYGLLFGVADTWAAMPDNKMVGKAIKDPEKLKIIAANPRRYTGPPRVGTMRELVRVTQYI 243
           LYGLLFGVADTW AMPD KMVGK+IKDPEKLKIIA+NPRRYTG PRVGTMREL RV QYI
Sbjct: 186 LYGLLFGVADTWPAMPDKKMVGKSIKDPEKLKIIASNPRRYTGSPRVGTMRELARVCQYI 245

Query: 244 RDNFSRVTAPFLTVHGTADGVTCPSSSELLYEKATSVDKTLKLYDGMYHSLIQGEPDENV 303
           +DNFS+VTAPFLTVHGT DGVTCPSSS+LLYEKA+SVDK+LKLYDGMYHSLIQGE DEN 
Sbjct: 246 QDNFSKVTAPFLTVHGTDDGVTCPSSSKLLYEKASSVDKSLKLYDGMYHSLIQGETDENA 305

Query: 304 EIVLRDMREWIDERALRYGPK 325
            +VLRDM+EWIDERA +YGPK
Sbjct: 306 SLVLRDMKEWIDERAEKYGPK 322

BLAST of CSPI03G25100 vs. TrEMBL
Match: A0A067D7D1_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g020633mg PE=4 SV=1)

HSP 1 Score: 558.1 bits (1437), Expect = 6.9e-156
Identity = 261/324 (80.56%), Postives = 291/324 (89.81%), Query Frame = 1

Query: 1   MPTESSPPPEPPLNFWGDQPEEEFYASQGVRNTKSFFETSHGKLFTQSFIPLDFPDLKGT 60
           MP E+    E P NFWGD PEEE+Y SQGVRN K +FET +GKLFTQSF+PLD   +K T
Sbjct: 1   MPPETQT--ETPPNFWGDMPEEEYYTSQGVRNGKKYFETPNGKLFTQSFLPLD-QKVKAT 60

Query: 61  VYMTHGYGSDTGWMFQKICLSYASWGYAVFAADLLGHGRSDGLRCYLGDMDKIAAASLSF 120
           VYMTHGYGSDTGWMFQKIC+S+A+WGYAVFAADLLGHGRSDG+RCYLGDM+K+AA+SLSF
Sbjct: 61  VYMTHGYGSDTGWMFQKICISFATWGYAVFAADLLGHGRSDGIRCYLGDMEKVAASSLSF 120

Query: 121 FLHTRRSEPYRHLPAFLFGESMGCAATMLMYLQSDPDTWTGLIFSAPLFVIPENMKPSKL 180
           F H R SEPYR LP FLFGESMG AATMLMY QS+P+TWTGLIFSAPLFVIPENMKPSKL
Sbjct: 121 FKHVRDSEPYRDLPGFLFGESMGGAATMLMYFQSEPNTWTGLIFSAPLFVIPENMKPSKL 180

Query: 181 RLFLYGLLFGVADTWAAMPDNKMVGKAIKDPEKLKIIAANPRRYTGPPRVGTMRELVRVT 240
            LF+YGLLFG+ADTWAAMPDNKMVGKAIKDPEKLK+IA+NPRRYTG PRVGTMRE+ RV 
Sbjct: 181 HLFMYGLLFGLADTWAAMPDNKMVGKAIKDPEKLKVIASNPRRYTGKPRVGTMREIARVC 240

Query: 241 QYIRDNFSRVTAPFLTVHGTADGVTCPSSSELLYEKATSVDKTLKLYDGMYHSLIQGEPD 300
           QYI+DNFS+VT PFLTVHGTADGVTCP+SS+LLYEKA+S DK++K+YDGMYHSLIQGEPD
Sbjct: 241 QYIQDNFSKVTVPFLTVHGTADGVTCPTSSKLLYEKASSADKSIKIYDGMYHSLIQGEPD 300

Query: 301 ENVEIVLRDMREWIDERALRYGPK 325
           EN  +VL+DMREWIDER  RYGPK
Sbjct: 301 ENANLVLKDMREWIDERVERYGPK 321

BLAST of CSPI03G25100 vs. TAIR10
Match: AT1G52760.1 (AT1G52760.1 lysophospholipase 2)

HSP 1 Score: 535.4 bits (1378), Expect = 2.4e-152
Identity = 244/316 (77.22%), Postives = 283/316 (89.56%), Query Frame = 1

Query: 9   PEPPLNFWGDQPEEEFYASQGVRNTKSFFETSHGKLFTQSFIPLDFPDLKGTVYMTHGYG 68
           P PP NFWG  PEEE+Y SQGVRN+KS+FET +GKLFTQSF+PLD  ++KGTVYM+HGYG
Sbjct: 16  PPPPPNFWGTMPEEEYYTSQGVRNSKSYFETPNGKLFTQSFLPLD-GEIKGTVYMSHGYG 75

Query: 69  SDTGWMFQKICLSYASWGYAVFAADLLGHGRSDGLRCYLGDMDKIAAASLSFFLHTRRSE 128
           SDT WMFQKIC+S++SWGYAVFAADLLGHGRSDG+RCY+GDM+K+AA SL+FF H R S+
Sbjct: 76  SDTSWMFQKICMSFSSWGYAVFAADLLGHGRSDGIRCYMGDMEKVAATSLAFFKHVRCSD 135

Query: 129 PYRHLPAFLFGESMGCAATMLMYLQSDPDTWTGLIFSAPLFVIPENMKPSKLRLFLYGLL 188
           PY+ LPAFLFGESMG   T+LMY QS+P+TWTGL+FSAPLFVIPE+MKPSK  LF YGLL
Sbjct: 136 PYKDLPAFLFGESMGGLVTLLMYFQSEPETWTGLMFSAPLFVIPEDMKPSKAHLFAYGLL 195

Query: 189 FGVADTWAAMPDNKMVGKAIKDPEKLKIIAANPRRYTGPPRVGTMRELVRVTQYIRDNFS 248
           FG+ADTWAAMPDNKMVGKAIKDPEKLKIIA+NP+RYTG PRVGTMREL+R TQY+++NF 
Sbjct: 196 FGLADTWAAMPDNKMVGKAIKDPEKLKIIASNPQRYTGKPRVGTMRELLRKTQYVQENFG 255

Query: 249 RVTAPFLTVHGTADGVTCPSSSELLYEKATSVDKTLKLYDGMYHSLIQGEPDENVEIVLR 308
           +VT P  T HGTADGVTCP+SS+LLYEKA+S DKTLK+Y+GMYHSLIQGEPDEN EIVL+
Sbjct: 256 KVTIPVFTAHGTADGVTCPTSSKLLYEKASSADKTLKIYEGMYHSLIQGEPDENAEIVLK 315

Query: 309 DMREWIDERALRYGPK 325
           DMREWIDE+  +YG K
Sbjct: 316 DMREWIDEKVKKYGSK 330

BLAST of CSPI03G25100 vs. TAIR10
Match: AT1G11090.1 (AT1G11090.1 alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 260.4 bits (664), Expect = 1.5e-69
Identity = 131/310 (42.26%), Postives = 193/310 (62.26%), Query Frame = 1

Query: 10  EPPLNFWGDQPEEEFYASQGVRNTKSFFETSHG-KLFTQSFIPLDFPDLKGTVYMTHGYG 69
           E  +NFWG+  EE+++  +G+  +KSFF +  G  LFT+S++P      +G ++M HGYG
Sbjct: 7   ETEINFWGETSEEDYFNLKGIIGSKSFFTSPRGLNLFTRSWLPSSSSPPRGLIFMVHGYG 66

Query: 70  SDTGWMFQKICLSYASWGYAVFAADLLGHGRSDGLRCYLGDMDKIAAASLSFFLHTRRSE 129
           +D  W FQ   +  A  G+A FA D+ GHGRSDG+R Y+  +D +    +SFF   +++ 
Sbjct: 67  NDVSWTFQSTPIFLAQMGFACFALDIEGHGRSDGVRAYVPSVDLVVDDIISFFNSIKQNP 126

Query: 130 PYRHLPAFLFGESMGCAATMLMYLQSDPDTWTGLIFSAPLFVIPENMKPS-KLRLFLYGL 189
            ++ LP FLFGESMG A  +L+   +DP  + G +  AP+  I + ++P   +  FL  +
Sbjct: 127 KFQGLPRFLFGESMGGAICLLIQF-ADPLGFDGAVLVAPMCKISDKVRPKWPVDQFLI-M 186

Query: 190 LFGVADTWAAMPDNKMVGKAIKDPEKLKIIAANPRRYTGPPRVGTMRELVRVTQYIRDNF 249
           +     TWA +P   ++ K+IK  EK  I   NP RY   PR+GT+ EL+RVT Y+    
Sbjct: 187 ISRFLPTWAIVPTEDLLEKSIKVEEKKPIAKRNPMRYNEKPRLGTVMELLRVTDYLGKKL 246

Query: 250 SRVTAPFLTVHGTADGVTCPSSSELLYEKATSVDKTLKLYDGMYHSLIQGEPDENVEIVL 309
             V+ PF+ VHG+AD VT P  S  LYE A S DKTLK+YDGM HS++ GEPD+N+EIV 
Sbjct: 247 KDVSIPFIIVHGSADAVTDPEVSRELYEHAKSKDKTLKIYDGMMHSMLFGEPDDNIEIVR 306

Query: 310 RDMREWIDER 318
           +D+  W+++R
Sbjct: 307 KDIVSWLNDR 314

BLAST of CSPI03G25100 vs. TAIR10
Match: AT3G62860.1 (AT3G62860.1 alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 200.3 bits (508), Expect = 1.8e-51
Identity = 103/293 (35.15%), Postives = 155/293 (52.90%), Query Frame = 1

Query: 25  YASQGVRNTKSFFETSHGKLFTQSFIPLDFPDLKGTVYMTHGYGSDTGWMFQKICLSYAS 84
           Y  + ++N++        +LF   ++P   P  +  V++ HGYG +     ++  +  AS
Sbjct: 8   YEEEYIKNSRDV------ELFACRWLPSSSP--RALVFLCHGYGMECSSFMRECGIRLAS 67

Query: 85  WGYAVFAADLLGHGRSDGLRCYLGDMDKIAAASLSFFLHTRRSEPYRHLPAFLFGESMGC 144
            GYAVF  D  GHGRS G RCY+     I      ++      E Y+    FL+GESMG 
Sbjct: 68  AGYAVFGMDYEGHGRSKGARCYIKKFSNIVNDCFDYYTSISAQEEYKEKGRFLYGESMGG 127

Query: 145 AATMLMYLQSDPDTWTGLIFSAPLFVIPENMKPSKLRLFLYGLLFGVADTWAAMPDNKMV 204
           A  +L++ + DP  W G +  AP+  I E +KP  + + L   +  +   W  +P   ++
Sbjct: 128 AVALLLH-KKDPSFWNGALLVAPMCKISEKVKPHPVVINLLTRVEDIIPKWKIVPTKDVI 187

Query: 205 GKAIKDPEKLKIIAANPRRYTGPPRVGTMRELVRVTQYIRDNFSRVTAPFLTVHGTADGV 264
             A KDP K + I  N   Y   PR+ T  E++R +  + D    +T PF  +HG AD V
Sbjct: 188 DAAFKDPVKREEIRNNKLIYQDKPRLKTALEMLRTSMDLEDTLHEITLPFFVLHGEADIV 247

Query: 265 TCPSSSELLYEKATSVDKTLKLYDGMYHSLIQGEPDENVEIVLRDMREWIDER 318
           T P  S+ L+EKA++ DKT+KLY GM+H L  GEPD NV++V  D+  W+D R
Sbjct: 248 TDPEISKALFEKASTRDKTIKLYPGMWHGLTSGEPDANVDLVFADIVNWLDAR 291

BLAST of CSPI03G25100 vs. TAIR10
Match: AT2G47630.1 (AT2G47630.1 alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 197.6 bits (501), Expect = 1.2e-50
Identity = 107/293 (36.52%), Postives = 154/293 (52.56%), Query Frame = 1

Query: 25  YASQGVRNTKSFFETSHGKLFTQSFIPLDFPDLKGTVYMTHGYGSDTGWMFQKICLSYAS 84
           Y  + VRN++        +LF   +IP      K  V++ HGYG +     ++  +  AS
Sbjct: 8   YHEEYVRNSRGV------ELFACRWIPSSSSSPKALVFLCHGYGMECSDSMKECGIRLAS 67

Query: 85  WGYAVFAADLLGHGRSDGLRCYLGDMDKIAAASLSFFLHTRRSEPYRHLPAFLFGESMGC 144
            GYAVF  D  GHGRS G RCY+     +      ++      E Y     FL+GESMG 
Sbjct: 68  AGYAVFGMDYEGHGRSMGSRCYIKKFANVVNDCYDYYTSICAQEEYMDKGRFLYGESMGG 127

Query: 145 AATMLMYLQSDPDTWTGLIFSAPLFVIPENMKPSKLRLFLYGLLFGVADTWAAMPDNKMV 204
           A T+L++ + DP  W G I  AP+  I E +KP  + + L   +  +   W  +P   ++
Sbjct: 128 AVTLLLH-KKDPLFWNGAILVAPMCKISEKVKPHPIVINLLTRVEEIIPKWKIVPTKDVI 187

Query: 205 GKAIKDPEKLKIIAANPRRYTGPPRVGTMRELVRVTQYIRDNFSRVTAPFLTVHGTADGV 264
             A KD  K + +  N   Y   PR+ T  E++R +  + D    +T PF  +HG AD V
Sbjct: 188 DAAFKDLVKREEVRNNKLIYQDKPRLKTALEMLRTSMNLEDTLHEITMPFFVLHGEADTV 247

Query: 265 TCPSSSELLYEKATSVDKTLKLYDGMYHSLIQGEPDENVEIVLRDMREWIDER 318
           T P  S+ LYEKA++ DKTLKLY GM+H+L  GEPD NV++V  D+  W+D R
Sbjct: 248 TDPEVSKALYEKASTRDKTLKLYPGMWHALTSGEPDCNVDLVFADIINWLDLR 293

BLAST of CSPI03G25100 vs. TAIR10
Match: AT5G14980.1 (AT5G14980.1 alpha/beta-Hydrolases superfamily protein)

HSP 1 Score: 194.5 bits (493), Expect = 1.0e-49
Identity = 112/305 (36.72%), Postives = 163/305 (53.44%), Query Frame = 1

Query: 16  WGDQPEEEFYASQGVRNTKSFFETSHG-KLFTQSFIPLDFPDLKGTVYMTHGYGSDTGWM 75
           +G     EFY+   V ++ ++     G KLFTQ + PL+ P L G + + HG+  ++ W 
Sbjct: 15  FGSLSAAEFYSRHSVTHSSAYVTNPTGLKLFTQWWTPLNRPPL-GLIAVVHGFTGESSWF 74

Query: 76  FQKICLSYASWGYAVFAADLLGHGRSDGLRCYLGDMDKIAAASLSFFLHTRRSEPYRHLP 135
            Q   + +A  GY   A D  GHG SDGL  ++ +++ I    +SFF   R+      LP
Sbjct: 75  LQLTSVLFAKSGYLTCAIDHQGHGFSDGLTAHIPNINLIVDDCISFFDDFRKRHASSFLP 134

Query: 136 AFLFGESMGCAATMLMYLQSDPDTWTGLIFSAPLFVIPENMKPSKLRLFLYGLLFGVADT 195
           AFL+ ES+G A  + + L+     W GLI S  +  I    KP      L  L   +  T
Sbjct: 135 AFLYSESLGGAIALYITLRQKHQ-WNGLILSGAMCSISHKFKPPWPLQHLLTLAATLIPT 194

Query: 196 WAAMPDN-KMVGKAIKDPEKLKIIAANPRRYTGPPRVGTMRELVRVTQYIRDNFSRVTAP 255
           W  +P    + G + K+P K K+  ANP R  G PR  T  ELVRV + +++ F  V  P
Sbjct: 195 WRVVPTRGSIAGVSFKEPWKRKLAYANPNRTVGKPRAATAYELVRVCEDLQNRFEEVEVP 254

Query: 256 FLTVHGTADGVTCPSSSELLYEKATSVDKTLKLYDGMYHSLIQGEPDENVEIVLRDMREW 315
            + VHG  D V  P+S E LY + +S DKT+K+Y GM+H LI GE +ENV++V  D+ +W
Sbjct: 255 LMIVHGRDDVVCDPASVEELYRRCSSRDKTIKIYPGMWHQLI-GESEENVDLVFGDVLDW 314

Query: 316 IDERA 319
           I  R+
Sbjct: 315 IKTRS 316

BLAST of CSPI03G25100 vs. NCBI nr
Match: gi|449460529|ref|XP_004147998.1| (PREDICTED: caffeoylshikimate esterase [Cucumis sativus])

HSP 1 Score: 677.9 bits (1748), Expect = 8.6e-192
Identity = 324/325 (99.69%), Postives = 325/325 (100.00%), Query Frame = 1

Query: 1   MPTESSPPPEPPLNFWGDQPEEEFYASQGVRNTKSFFETSHGKLFTQSFIPLDFPDLKGT 60
           MPTESSPPPEPPLNFWGDQPEEEFYASQGVRNTKSFFETSHGKLFTQSFIPLDFPDLKGT
Sbjct: 1   MPTESSPPPEPPLNFWGDQPEEEFYASQGVRNTKSFFETSHGKLFTQSFIPLDFPDLKGT 60

Query: 61  VYMTHGYGSDTGWMFQKICLSYASWGYAVFAADLLGHGRSDGLRCYLGDMDKIAAASLSF 120
           VYMTHGYGSDTGWMFQKICLSYASWGYAVFAADLLGHGRSDGLRCYLGDMDKIAAASLSF
Sbjct: 61  VYMTHGYGSDTGWMFQKICLSYASWGYAVFAADLLGHGRSDGLRCYLGDMDKIAAASLSF 120

Query: 121 FLHTRRSEPYRHLPAFLFGESMGCAATMLMYLQSDPDTWTGLIFSAPLFVIPENMKPSKL 180
           FLHTRRSEPYRHLPAFLFGESMGCAATMLMYLQSDPDTWTGLIFSAPLFVIPENMKPSKL
Sbjct: 121 FLHTRRSEPYRHLPAFLFGESMGCAATMLMYLQSDPDTWTGLIFSAPLFVIPENMKPSKL 180

Query: 181 RLFLYGLLFGVADTWAAMPDNKMVGKAIKDPEKLKIIAANPRRYTGPPRVGTMRELVRVT 240
           RLFLYGLLFGVADTWAAMPDNKMVGKAIKDP+KLKIIAANPRRYTGPPRVGTMRELVRVT
Sbjct: 181 RLFLYGLLFGVADTWAAMPDNKMVGKAIKDPQKLKIIAANPRRYTGPPRVGTMRELVRVT 240

Query: 241 QYIRDNFSRVTAPFLTVHGTADGVTCPSSSELLYEKATSVDKTLKLYDGMYHSLIQGEPD 300
           QYIRDNFSRVTAPFLTVHGTADGVTCPSSSELLYEKATSVDKTLKLYDGMYHSLIQGEPD
Sbjct: 241 QYIRDNFSRVTAPFLTVHGTADGVTCPSSSELLYEKATSVDKTLKLYDGMYHSLIQGEPD 300

Query: 301 ENVEIVLRDMREWIDERALRYGPKK 326
           ENVEIVLRDMREWIDERALRYGPKK
Sbjct: 301 ENVEIVLRDMREWIDERALRYGPKK 325

BLAST of CSPI03G25100 vs. NCBI nr
Match: gi|659093321|ref|XP_008447481.1| (PREDICTED: caffeoylshikimate esterase [Cucumis melo])

HSP 1 Score: 650.6 bits (1677), Expect = 1.5e-183
Identity = 311/318 (97.80%), Postives = 313/318 (98.43%), Query Frame = 1

Query: 8   PPEPPLNFWGDQPEEEFYASQGVRNTKSFFETSHGKLFTQSFIPLDFPDLKGTVYMTHGY 67
           PPEPPLNFWGDQPEEEFYASQGVRNTKSFFET HGKLFTQSFIPLD PDLKGTVYMTHGY
Sbjct: 2   PPEPPLNFWGDQPEEEFYASQGVRNTKSFFETPHGKLFTQSFIPLDSPDLKGTVYMTHGY 61

Query: 68  GSDTGWMFQKICLSYASWGYAVFAADLLGHGRSDGLRCYLGDMDKIAAASLSFFLHTRRS 127
           GSDTGWMFQKICLSYASWGYAVFAADLLGHGRSDGLRCYLGDMDKIAAASLSFFLHTRRS
Sbjct: 62  GSDTGWMFQKICLSYASWGYAVFAADLLGHGRSDGLRCYLGDMDKIAAASLSFFLHTRRS 121

Query: 128 EPYRHLPAFLFGESMGCAATMLMYLQSDPDTWTGLIFSAPLFVIPENMKPSKLRLFLYGL 187
           EPYRHLPAFLFGESMG AATMLMY QSDPDTWTGLIFSAPLFVIPENMKPSKLRLFLYGL
Sbjct: 122 EPYRHLPAFLFGESMGGAATMLMYFQSDPDTWTGLIFSAPLFVIPENMKPSKLRLFLYGL 181

Query: 188 LFGVADTWAAMPDNKMVGKAIKDPEKLKIIAANPRRYTGPPRVGTMRELVRVTQYIRDNF 247
           LFGVADTWAAMPDNKMVGKAIKDPEKLKIIAANPRRYTGPPRVGTMRELVRVTQYIRDNF
Sbjct: 182 LFGVADTWAAMPDNKMVGKAIKDPEKLKIIAANPRRYTGPPRVGTMRELVRVTQYIRDNF 241

Query: 248 SRVTAPFLTVHGTADGVTCPSSSELLYEKATSVDKTLKLYDGMYHSLIQGEPDENVEIVL 307
           S+VTAPFLTVHGTADGVTCPSSS+LLYEKA SVDKTLKLYDGMYHSLIQGEPDENVEIVL
Sbjct: 242 SKVTAPFLTVHGTADGVTCPSSSQLLYEKAASVDKTLKLYDGMYHSLIQGEPDENVEIVL 301

Query: 308 RDMREWIDERALRYGPKK 326
           RDMREWIDERALRYGPKK
Sbjct: 302 RDMREWIDERALRYGPKK 319

BLAST of CSPI03G25100 vs. NCBI nr
Match: gi|1009113217|ref|XP_015872285.1| (PREDICTED: caffeoylshikimate esterase [Ziziphus jujuba])

HSP 1 Score: 582.4 bits (1500), Expect = 4.9e-163
Identity = 270/324 (83.33%), Postives = 302/324 (93.21%), Query Frame = 1

Query: 1   MPTESSPPPEPPLNFWGDQPEEEFYASQGVRNTKSFFETSHGKLFTQSFIPLDFPDLKGT 60
           M +E+ PP  PP NFWG+ PEEE+Y SQGVRNTKS+F+T HGK+FTQSF+PLD P++KGT
Sbjct: 1   MQSETLPPETPP-NFWGNMPEEEYYTSQGVRNTKSYFDTPHGKIFTQSFLPLDTPNVKGT 60

Query: 61  VYMTHGYGSDTGWMFQKICLSYASWGYAVFAADLLGHGRSDGLRCYLGDMDKIAAASLSF 120
           VYM+HGYGSDTGW+FQKIC+S+++WGYAVFAADLLGHGRSDGLRCYLGDM+K+AAASLS+
Sbjct: 61  VYMSHGYGSDTGWLFQKICISFSTWGYAVFAADLLGHGRSDGLRCYLGDMEKVAAASLSY 120

Query: 121 FLHTRRSEPYRHLPAFLFGESMGCAATMLMYLQSDPDTWTGLIFSAPLFVIPENMKPSKL 180
           F+H RRSEPY+ LPAFLFGESMG AATMLMY QSDPDTWTGLIFSAPLFV+PENMKPSK+
Sbjct: 121 FVHVRRSEPYKDLPAFLFGESMGGAATMLMYFQSDPDTWTGLIFSAPLFVMPENMKPSKV 180

Query: 181 RLFLYGLLFGVADTWAAMPDNKMVGKAIKDPEKLKIIAANPRRYTGPPRVGTMRELVRVT 240
           RLFLYG+LFG+ADTWAAMPDNKMVGKAIKDPEKLKIIA+NPRRYTG PRVGTMREL RV 
Sbjct: 181 RLFLYGMLFGIADTWAAMPDNKMVGKAIKDPEKLKIIASNPRRYTGQPRVGTMRELARVC 240

Query: 241 QYIRDNFSRVTAPFLTVHGTADGVTCPSSSELLYEKATSVDKTLKLYDGMYHSLIQGEPD 300
           QYI+DNFS+VTAPFLTVHGTADGVTCP+SS+LLYEKA+S DK+LKLYDGMYHSLIQGEPD
Sbjct: 241 QYIQDNFSKVTAPFLTVHGTADGVTCPTSSQLLYEKASSADKSLKLYDGMYHSLIQGEPD 300

Query: 301 ENVEIVLRDMREWIDERALRYGPK 325
           EN  IVLRDMREWIDER  RYG K
Sbjct: 301 ENANIVLRDMREWIDERVERYGSK 323

BLAST of CSPI03G25100 vs. NCBI nr
Match: gi|1006615644|gb|AMQ49208.1| (caffeoylshikimate esterase [Ziziphus jujuba])

HSP 1 Score: 581.3 bits (1497), Expect = 1.1e-162
Identity = 269/324 (83.02%), Postives = 302/324 (93.21%), Query Frame = 1

Query: 1   MPTESSPPPEPPLNFWGDQPEEEFYASQGVRNTKSFFETSHGKLFTQSFIPLDFPDLKGT 60
           M +E+ PP  PP NFWG+ PEEE+Y SQGVRNTKS+F+T HGK+FTQSF+PLD P++KGT
Sbjct: 1   MQSETLPPETPP-NFWGNMPEEEYYTSQGVRNTKSYFDTPHGKIFTQSFLPLDTPNVKGT 60

Query: 61  VYMTHGYGSDTGWMFQKICLSYASWGYAVFAADLLGHGRSDGLRCYLGDMDKIAAASLSF 120
           VYM+HGYGSDTGW+FQKIC+S+++WGYAVFAADLLGHGRSDGLRCYLGD++K+AAASLS+
Sbjct: 61  VYMSHGYGSDTGWLFQKICISFSTWGYAVFAADLLGHGRSDGLRCYLGDLEKVAAASLSY 120

Query: 121 FLHTRRSEPYRHLPAFLFGESMGCAATMLMYLQSDPDTWTGLIFSAPLFVIPENMKPSKL 180
           F+H RRSEPY+ LPAFLFGESMG AATMLMY QSDPDTWTGLIFSAPLFV+PENMKPSK+
Sbjct: 121 FVHVRRSEPYKDLPAFLFGESMGGAATMLMYFQSDPDTWTGLIFSAPLFVMPENMKPSKV 180

Query: 181 RLFLYGLLFGVADTWAAMPDNKMVGKAIKDPEKLKIIAANPRRYTGPPRVGTMRELVRVT 240
           RLFLYG+LFG+ADTWAAMPDNKMVGKAIKDPEKLKIIA+NPRRYTG PRVGTMREL RV 
Sbjct: 181 RLFLYGMLFGIADTWAAMPDNKMVGKAIKDPEKLKIIASNPRRYTGQPRVGTMRELARVC 240

Query: 241 QYIRDNFSRVTAPFLTVHGTADGVTCPSSSELLYEKATSVDKTLKLYDGMYHSLIQGEPD 300
           QYI+DNFS+VTAPFLTVHGTADGVTCP+SS+LLYEKA+S DK+LKLYDGMYHSLIQGEPD
Sbjct: 241 QYIQDNFSKVTAPFLTVHGTADGVTCPTSSQLLYEKASSADKSLKLYDGMYHSLIQGEPD 300

Query: 301 ENVEIVLRDMREWIDERALRYGPK 325
           EN  IVLRDMREWIDER  RYG K
Sbjct: 301 ENANIVLRDMREWIDERVERYGSK 323

BLAST of CSPI03G25100 vs. NCBI nr
Match: gi|595857341|ref|XP_007210524.1| (hypothetical protein PRUPE_ppa008845mg [Prunus persica])

HSP 1 Score: 572.0 bits (1473), Expect = 6.6e-160
Identity = 267/315 (84.76%), Postives = 291/315 (92.38%), Query Frame = 1

Query: 10  EPPLNFWGDQPEEEFYASQGVRNTKSFFETSHGKLFTQSFIPLDFPDLKGTVYMTHGYGS 69
           E P NFWGD PEE++Y SQGVRNTKSFF+T +G++FTQSF+PLD   +KGTV+MTHGYGS
Sbjct: 4   ETPPNFWGDMPEEDYYTSQGVRNTKSFFQTPNGQIFTQSFLPLD-QTVKGTVFMTHGYGS 63

Query: 70  DTGWMFQKICLSYASWGYAVFAADLLGHGRSDGLRCYLGDMDKIAAASLSFFLHTRRSEP 129
           DTGW+FQKIC+ YA+WGYAVFAADLLGHGRSDGLRCYLGDM+K+AA SLSFFLH RR+E 
Sbjct: 64  DTGWLFQKICMHYATWGYAVFAADLLGHGRSDGLRCYLGDMEKVAATSLSFFLHVRRNEL 123

Query: 130 YRHLPAFLFGESMGCAATMLMYLQSDPDTWTGLIFSAPLFVIPENMKPSKLRLFLYGLLF 189
           Y HLPAFLFGESMG AATMLMY QS PDTWTGLIFSAPLFVIPENMKPSK+ LFLYGLLF
Sbjct: 124 YLHLPAFLFGESMGGAATMLMYFQSPPDTWTGLIFSAPLFVIPENMKPSKVHLFLYGLLF 183

Query: 190 GVADTWAAMPDNKMVGKAIKDPEKLKIIAANPRRYTGPPRVGTMRELVRVTQYIRDNFSR 249
           G+ADTWAAMPDNKMVGKAIKDP KL+IIA+NPRRYTGPPRVGTMRE+ RV QYI+DNFSR
Sbjct: 184 GIADTWAAMPDNKMVGKAIKDPAKLRIIASNPRRYTGPPRVGTMREIARVCQYIQDNFSR 243

Query: 250 VTAPFLTVHGTADGVTCPSSSELLYEKATSVDKTLKLYDGMYHSLIQGEPDENVEIVLRD 309
           VT PFLTVHGTADGVTCPSSS+LLYEKA+SVDKTLK+YDGMYHSLIQGEPDEN EIVLRD
Sbjct: 244 VTTPFLTVHGTADGVTCPSSSKLLYEKASSVDKTLKIYDGMYHSLIQGEPDENAEIVLRD 303

Query: 310 MREWIDERALRYGPK 325
           MREWIDER  RYGPK
Sbjct: 304 MREWIDERVERYGPK 317

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
CSE_ARATH4.3e-15177.22Caffeoylshikimate esterase OS=Arabidopsis thaliana GN=CSE PE=1 SV=1[more]
MGLL_MOUSE2.5e-1826.07Monoglyceride lipase OS=Mus musculus GN=Mgll PE=1 SV=1[more]
MGLL_RAT5.6e-1827.17Monoglyceride lipase OS=Rattus norvegicus GN=Mgll PE=1 SV=1[more]
Y9086_DICDI1.1e-1627.44Uncharacterized abhydrolase domain-containing protein DDB_G0269086 OS=Dictyostel... [more]
MGLL_SCHPO4.5e-0728.79Putative monoglyceride lipase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24... [more]
Match NameE-valueIdentityDescription
A0A0A0LBT8_CUCSA6.0e-19299.69Uncharacterized protein OS=Cucumis sativus GN=Csa_3G539670 PE=4 SV=1[more]
A0A142EHU3_ZIZJJ7.6e-16383.02Caffeoylshikimate esterase OS=Ziziphus jujuba PE=2 SV=1[more]
M5WQY3_PRUPE4.6e-16084.76Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa008845mg PE=4 SV=1[more]
W9RZF8_9ROSA6.2e-15781.00Uncharacterized protein OS=Morus notabilis GN=L484_003175 PE=4 SV=1[more]
A0A067D7D1_CITSI6.9e-15680.56Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g020633mg PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT1G52760.12.4e-15277.22 lysophospholipase 2[more]
AT1G11090.11.5e-6942.26 alpha/beta-Hydrolases superfamily protein[more]
AT3G62860.11.8e-5135.15 alpha/beta-Hydrolases superfamily protein[more]
AT2G47630.11.2e-5036.52 alpha/beta-Hydrolases superfamily protein[more]
AT5G14980.11.0e-4936.72 alpha/beta-Hydrolases superfamily protein[more]
Match NameE-valueIdentityDescription
gi|449460529|ref|XP_004147998.1|8.6e-19299.69PREDICTED: caffeoylshikimate esterase [Cucumis sativus][more]
gi|659093321|ref|XP_008447481.1|1.5e-18397.80PREDICTED: caffeoylshikimate esterase [Cucumis melo][more]
gi|1009113217|ref|XP_015872285.1|4.9e-16383.33PREDICTED: caffeoylshikimate esterase [Ziziphus jujuba][more]
gi|1006615644|gb|AMQ49208.1|1.1e-16283.02caffeoylshikimate esterase [Ziziphus jujuba][more]
gi|595857341|ref|XP_007210524.1|6.6e-16084.76hypothetical protein PRUPE_ppa008845mg [Prunus persica][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR022742Hydrolase_4
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042967 acyl-carrier-protein biosynthetic process
biological_process GO:0046486 glycerolipid metabolic process
biological_process GO:0046686 response to cadmium ion
biological_process GO:0042542 response to hydrogen peroxide
biological_process GO:0008150 biological_process
cellular_component GO:0005886 plasma membrane
cellular_component GO:0009506 plasmodesma
cellular_component GO:0005575 cellular_component
molecular_function GO:0003846 2-acylglycerol O-acyltransferase activity
molecular_function GO:0090430 caffeoyl-CoA: alcohol caffeoyl transferase activity
molecular_function GO:0004622 lysophospholipase activity
molecular_function GO:0003674 molecular_function

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI03G25100.1CSPI03G25100.1mRNA


Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR022742Serine aminopeptidase, S33PFAMPF12146Hydrolase_4coord: 57..299
score: 5.1
NoneNo IPR availablePANTHERPTHR11614PHOSPHOLIPASE-RELATEDcoord: 9..325
score: 1.9E
NoneNo IPR availablePANTHERPTHR11614:SF90CAFFEOYLSHIKIMATE ESTERASEcoord: 9..325
score: 1.9E