CSPI03G25060 (gene) Wild cucumber (PI 183967) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CSPI03G25060
Type	gene
Organism	Cucumis sativus (Wild cucumber (PI 183967))
Description	Retrovirus-related Pol polyprotein from transposon TNT 1-94
Location	Chr3 : 22281087 .. 22282403 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACAGGAAAAAGATGGAATAGAACTGATGTAGTTGTGGACAACATTTTTGTATATAATGTTGCACATAATATCACTCATGAAAATGAGGATTCTGAACCTAAATCTGTTGAAGAATGTTGTAATAGAAAGGATTGGCCCAAGTGGAAAGAAGTCATCCAAGCAAAATTGAACTCACTTTCAAAACGTGAAGTTTGTGGACCTGTAGTTCATACACCTAAAGGTGTAAAACCTATGGGATTTAAATGGGTATTTGTGCATAAAAGAAATGAAAATAATGAGGTCACTAGATATAAAGCACGACTTGTTGCACAAGGATTTTCTCAAAGACGGGGCATTGATTATGAGGAAACGTATTCTCATGTAGTGGATGTCATTACATTAAGATATCTAATTAGTCTGACTGTATGTGAAAAGCTTGATATGCATCTTATGGATGTAGTTACAACATATCTGTATGGATTTTTGGAAAATGAGATTTATATGAAAATCCCTGAAGGATTTAAGATACTTGAATCATATAATTCAAAATCTAGAGAATTATATTCAATCAAATTACAAAGATCATTATATGGATTGAAACAATCAGGACGAATGTGGTACAATCGCTTAAGTGAATATTTATTGAAGGAAGGTTATCAAAATAACCCAATTTGTCCATGTGTTTTTATTAAGAAATCACAGTCAAGATTTGCTATTATAGTTGTATATGTTGATGATTTAAATATAATTGGAACTCCTAAGAGCTTTCAAAGTCAATAGAATATTTCAAAAATGAATTCAAGATGAAAGATCTTAGCAAAACAAAATTTTGCCTTGGCTTGCAAATCGAGCATTTAGCTAATGGAATTTTTATTCATCAATCGACATATACATAAAAGATTTTAAAAAGATTCTACATGGACAAAGCACACCCATTGAACATTTCAATGGTGGTTCGATCACTAGATGTGAAAAGGGACATCTTTCGACCTCAAGAAGATAATGAAGAATTACTTGGTTCTGAAGTACCATACTTTAGTGCAATAGGTGCACTAATGTATCTTGCTAATAACACGAGACCATATATAGCATTTTCAGTAAATTTATTAGCAAGATATAATTCTTCTCCAACGAAAAGACATTGGAATGAAATTAAGCATGTACTTCGTTATCTTCGAGGGGCAATTGATATGGTTTTGTTTTATTCAAACAAATCAAACTTTGATTTAGTTGGTTATGCAGATGTTGGATATTTATATGATCCACATAAAGCAAGATCTCAAACAGGTTATCTGTTTACATGTGGAGGAACGACTATATCTTGGCGATTTGTAA

mRNA sequence

ATGACAGGAAAAAGATGGAATAGAACTGATGTAGTTGTGGACAACATTTTTGTATATAATGTTGCACATAATATCACTCATGAAAATGAGGATTCTGAACCTAAATCTGTTGAAGAATGTTGTAATAGAAAGGATTGGCCCAAGTGGAAAGAAGTCATCCAAGCAAAATTGAACTCACTTTCAAAACGTGAAGTTTGTGGACCTGTAGTTCATACACCTAAAGGTGTAAAACCTATGGGATTTAAATGGGTATTTGTGCATAAAAGAAATGAAAATAATGAGGTCACTAGATATAAAGCACGACTTGTTGCACAAGGATTTTCTCAAAGACGGGGCATTGATTATGAGGAAACGTATTCTCATGTAGTGGATGTCATTACATTAAGATATCTAATTAGTCTGACTGTATGTGAAAAGCTTGATATGCATCTTATGGATGTAGTTACAACATATCTGTATGGATTTTTGGAAAATGAGATTTATATGAAAATCCCTGAAGGATTTAAGATACTTGAATCATATAATTCAAAATCTAGAGAATTATATTCAATCAAATTACAAAGATCATTATATGGATTGAAACAATCAGGACGAATGTGGTACAATCGCTTAAGTGAATATTTATTGAAGGAAGATGTGAAAAGGGACATCTTTCGACCTCAAGAAGATAATGAAGAATTACTTGGTTCTGAAATGTTGGATATTTATATGATCCACATAAAGCAAGATCTCAAACAGGTTATCTGTTTACATGTGGAGGAACGACTATATCTTGGCGATTTGTAA

Coding sequence (CDS)

ATGACAGGAAAAAGATGGAATAGAACTGATGTAGTTGTGGACAACATTTTTGTATATAATGTTGCACATAATATCACTCATGAAAATGAGGATTCTGAACCTAAATCTGTTGAAGAATGTTGTAATAGAAAGGATTGGCCCAAGTGGAAAGAAGTCATCCAAGCAAAATTGAACTCACTTTCAAAACGTGAAGTTTGTGGACCTGTAGTTCATACACCTAAAGGTGTAAAACCTATGGGATTTAAATGGGTATTTGTGCATAAAAGAAATGAAAATAATGAGGTCACTAGATATAAAGCACGACTTGTTGCACAAGGATTTTCTCAAAGACGGGGCATTGATTATGAGGAAACGTATTCTCATGTAGTGGATGTCATTACATTAAGATATCTAATTAGTCTGACTGTATGTGAAAAGCTTGATATGCATCTTATGGATGTAGTTACAACATATCTGTATGGATTTTTGGAAAATGAGATTTATATGAAAATCCCTGAAGGATTTAAGATACTTGAATCATATAATTCAAAATCTAGAGAATTATATTCAATCAAATTACAAAGATCATTATATGGATTGAAACAATCAGGACGAATGTGGTACAATCGCTTAAGTGAATATTTATTGAAGGAAGATGTGAAAAGGGACATCTTTCGACCTCAAGAAGATAATGAAGAATTACTTGGTTCTGAAATGTTGGATATTTATATGATCCACATAAAGCAAGATCTCAAACAGGTTATCTGTTTACATGTGGAGGAACGACTATATCTTGGCGATTTGTAA

Homology

[Close]

BLAST of CSPI03G25060 vs. Swiss-Prot
Match: POLX_TOBAC (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 1.7e-28
Identity = 67/180 (37.22%), Postives = 113/180 (62.78%), Query Frame = 1


Query: 29  NEDSEPKSVEECCNRKDWPKWKEVIQAKLNSLSKREVCGPVVHTPKGVKPMGFKWVFVHK 88
           ++D EP+S++E  +  +  +  + +Q ++ SL K      +V  PKG +P+  KWVF  K
Sbjct: 806 SDDREPESLKEVLSHPEKNQLMKAMQEEMESLQKNGTY-KLVELPKGKRPLKCKWVFKLK 865

Query: 89  RNENNEVTRYKARLVAQGFSQRRGIDYEETYSHVVDVITLRYLISLTVCEKLDMHLMDVV 148
           ++ + ++ RYKARLV +GF Q++GID++E +S VV + ++R ++SL     L++  +DV 
Sbjct: 866 KDGDCKLVRYKARLVVKGFEQKKGIDFDEIFSPVVKMTSIRTILSLAASLDLEVEQLDVK 925

Query: 149 TTYLYGFLENEIYMKIPEGFKILESYNSKSRELYSIKLQRSLYGLKQSGRMWYNRLSEYL 208
           T +L+G LE EIYM+ PEGF++        ++    KL +SLYGLKQ+ R WY +   ++
Sbjct: 926 TAFLHGDLEEEIYMEQPEGFEV------AGKKHMVCKLNKSLYGLKQAPRQWYMKFDSFM 978

[Close]

BLAST of CSPI03G25060 vs. Swiss-Prot
Match: COPIA_DROME (Copia protein OS=Drosophila melanogaster GN=GIP PE=1 SV=3)

HSP 1 Score: 115.9 bits (289), Expect = 6.5e-25
Identity = 70/178 (39.33%), Postives = 102/178 (57.30%), Query Frame = 1


Query: 34   PKSVEECCNRKDWPKWKEVIQAKLNSLSKREVCGPVVHTPKGVKPMGFKWVFVHKRNENN 93
            P S +E   R D   W+E I  +LN+  K      +   P+    +  +WVF  K NE  
Sbjct: 891  PNSFDEIQYRDDKSSWEEAINTELNA-HKINNTWTITKRPENKNIVDSRWVFSVKYNELG 950

Query: 94   EVTRYKARLVAQGFSQRRGIDYEETYSHVVDVITLRYLISLTVCEKLDMHLMDVVTTYLY 153
               RYKARLVA+GF+Q+  IDYEET++ V  + + R+++SL +   L +H MDV T +L 
Sbjct: 951  NPIRYKARLVARGFTQKYQIDYEETFAPVARISSFRFILSLVIQYNLKVHQMDVKTAFLN 1010

Query: 154  GFLENEIYMKIPEGFKILESYNSKSRELYSIKLQRSLYGLKQSGRMWYNRLSEYLLKE 212
            G L+ EIYM++P+G     S NS +      KL +++YGLKQ+ R W+  + E  LKE
Sbjct: 1011 GTLKEEIYMRLPQGI----SCNSDN----VCKLNKAIYGLKQAARCWF-EVFEQALKE 1058

[Close]

BLAST of CSPI03G25060 vs. Swiss-Prot
Match: M820_ARATH (Uncharacterized mitochondrial protein AtMg00820 OS=Arabidopsis thaliana GN=AtMg00820 PE=4 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 2.3e-09
Identity = 44/110 (40.00%), Postives = 64/110 (58.18%), Query Frame = 1


Query: 33  EPKSVEECCNRKDWPKWKEVIQAKLNSLSKREVCGPVVHTPKGVKPMGFKWVFVHKRNEN 92
           EPKSV      KD P W + +Q +L++LS+ +    +V  P     +G KWVF  K + +
Sbjct: 27  EPKSVIFAL--KD-PGWCQAMQEELDALSRNKTW-ILVPPPVNQNILGCKWVFKTKLHSD 86

Query: 93  NEVTRYKARLVAQGFSQRRGIDYEETYSHVVDVITLRYLISLTVCEKLDM 143
             + R KARLVA+GF Q  GI + ETYS VV   T+R +  L V ++L++
Sbjct: 87  GTLDRLKARLVAKGFHQEEGIYFVETYSPVVRTATIRTI--LNVAQQLEV 130

[Close]

BLAST of CSPI03G25060 vs. Swiss-Prot
Match: YO11B_YEAST (Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=TY1B-OL PE=3 SV=1)

HSP 1 Score: 55.1 bits (131), Expect = 1.4e-06
Identity = 35/114 (30.70%), Postives = 57/114 (50.00%), Query Frame = 1


Query: 98   YKARLVAQGFSQRRGIDYEETYSHVVDVITLRYLISLTVCEKLDMHLMDVVTTYLYGFLE 157
            +KAR VA+G  Q          S+ V    L   +SL +     +  +D+ + YLY  ++
Sbjct: 1298 HKARFVARGDIQHPDTYDSGMQSNTVHHYALMTSLSLALDNNYYITQLDISSAYLYADIK 1357

Query: 158  NEIYMKIPEGFKILESYNSKSRELYSIKLQRSLYGLKQSGRMWYNRLSEYLLKE 212
             E+Y++ P    + +           I+L++SLYGLKQSG  WY  +  YL+K+
Sbjct: 1358 EELYIRPPPHLGMNDKL---------IRLKKSLYGLKQSGANWYETIKSYLIKQ 1402

[Close]

BLAST of CSPI03G25060 vs. Swiss-Prot
Match: YA11B_YEAST (Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=TY1B-A PE=3 SV=3)

HSP 1 Score: 55.1 bits (131), Expect = 1.4e-06
Identity = 37/119 (31.09%), Postives = 61/119 (51.26%), Query Frame = 1


Query: 98  YKARLVAQGFSQRRGIDYEETY-----SHVVDVITLRYLISLTVCEKLDMHLMDVVTTYL 157
           +KAR VA+G      I + +TY     S+ V    L   +SL +     +  +D+ + YL
Sbjct: 739 HKARFVARG-----DIQHPDTYDTGMQSNTVHHYALMTSLSLALDNNYYITQLDISSAYL 798

Query: 158 YGFLENEIYMKIPEGFKILESYNSKSRELYSIKLQRSLYGLKQSGRMWYNRLSEYLLKE 212
           Y  ++ E+Y++ P    + +           I+L++SLYGLKQSG  WY  +  YL+K+
Sbjct: 799 YADIKEELYIRPPPHLGMNDKL---------IRLKKSLYGLKQSGANWYETIKSYLIKQ 843

[Close]

BLAST of CSPI03G25060 vs. TrEMBL
Match: A5B4M9_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_015758 PE=4 SV=1)

HSP 1 Score: 317.4 bits (812), Expect = 1.6e-83
Identity = 150/210 (71.43%), Postives = 180/210 (85.71%), Query Frame = 1


Query: 2    TGKRWNRTDVVVDNIFVYNVAHNITHENEDSEPKSVEECCNRKDWPKWKEVIQAKLNSLS 61
            TG++W+R ++V++NIF + VA +I   +ED EP++VEEC +R DWPKWKE IQA+LNSL+
Sbjct: 803  TGEKWDRNNIVINNIFAFQVASDIIRNDEDPEPRNVEECRHRNDWPKWKEXIQAELNSLT 862

Query: 62   KREVCGPVVHTPKGVKPMGFKWVFVHKRNENNEVTRYKARLVAQGFSQRRGIDYEETYSH 121
            KREV GPVV TP+ VKP+G+KWVFV KRNENNE+ RYKARLVAQGFSQR GIDYEETYS 
Sbjct: 863  KREVFGPVVQTPEDVKPVGYKWVFVRKRNENNEIIRYKARLVAQGFSQRPGIDYEETYSP 922

Query: 122  VVDVITLRYLISLTVCEKLDMHLMDVVTTYLYGFLENEIYMKIPEGFKILESYNSKSREL 181
            V+D IT R+LISL V E LDM LMDV+T YLYG ++N+IYMKIPEGFK+ ++ N+K R +
Sbjct: 923  VMDAITFRFLISLAVSEGLDMRLMDVITAYLYGSMDNDIYMKIPEGFKLPDANNTKPRSM 982

Query: 182  YSIKLQRSLYGLKQSGRMWYNRLSEYLLKE 212
            YSIKLQRSLYGLKQSGRMWYNRLSEYLLKE
Sbjct: 983  YSIKLQRSLYGLKQSGRMWYNRLSEYLLKE 1012

[Close]

BLAST of CSPI03G25060 vs. TrEMBL
Match: A5AK32_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_038102 PE=4 SV=1)

HSP 1 Score: 312.0 bits (798), Expect = 6.9e-82
Identity = 147/210 (70.00%), Postives = 178/210 (84.76%), Query Frame = 1


Query: 2   TGKRWNRTDVVVDNIFVYNVAHNITHENEDSEPKSVEECCNRKDWPKWKEVIQAKLNSLS 61
           TG++W+R +++++NIF + VA +I    ED EP++VEEC +R DWPKWKE IQA+LNSL+
Sbjct: 135 TGEKWDRNNIIINNIFAFQVASDIIRNXEDPEPRNVEECRHRNDWPKWKEAIQAELNSLT 194

Query: 62  KREVCGPVVHTPKGVKPMGFKWVFVHKRNENNEVTRYKARLVAQGFSQRRGIDYEETYSH 121
           KREV GP+V TP+ VKP+G+KWVFV K NENNE+ RYKARLVAQGFSQR GIDYEETYS 
Sbjct: 195 KREVFGPIVQTPEDVKPVGYKWVFVRKPNENNEIIRYKARLVAQGFSQRPGIDYEETYSP 254

Query: 122 VVDVITLRYLISLTVCEKLDMHLMDVVTTYLYGFLENEIYMKIPEGFKILESYNSKSREL 181
           ++D IT R+LISL V E LDM LMDV+TTYLYG ++N+IYMKIPEGFK+ E+ N+K R +
Sbjct: 255 IMDAITFRFLISLAVLEGLDMCLMDVITTYLYGSMDNDIYMKIPEGFKLPEANNTKPRSM 314

Query: 182 YSIKLQRSLYGLKQSGRMWYNRLSEYLLKE 212
           YSIKLQRSLYGLKQSGRMWYN LSEYLLKE
Sbjct: 315 YSIKLQRSLYGLKQSGRMWYNCLSEYLLKE 344

[Close]

BLAST of CSPI03G25060 vs. TrEMBL
Match: A5B8K5_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_030674 PE=4 SV=1)

HSP 1 Score: 310.8 bits (795), Expect = 1.5e-81
Identity = 146/210 (69.52%), Postives = 180/210 (85.71%), Query Frame = 1


Query: 2   TGKRWNRTDVVVDNIFVYNVAHNITHENEDSEPKSVEECCNRKDWPKWKEVIQAKLNSLS 61
           TG++W+  ++V++NIF + VA +I    ED +P+SVEEC +R DWPKWKEVIQA+LNSL+
Sbjct: 108 TGEKWDWNNIVINNIFAFQVASDIIRNYEDLKPRSVEECRHRNDWPKWKEVIQAELNSLT 167

Query: 62  KREVCGPVVHTPKGVKPMGFKWVFVHKRNENNEVTRYKARLVAQGFSQRRGIDYEETYSH 121
           KRE+ GP+V TP+ VKP+G+KWVFV K +ENNE+ RYKARLVAQGFSQR G+DYEETYS 
Sbjct: 168 KREIFGPIVKTPEDVKPIGYKWVFVRKCDENNEIIRYKARLVAQGFSQRPGVDYEETYSP 227

Query: 122 VVDVITLRYLISLTVCEKLDMHLMDVVTTYLYGFLENEIYMKIPEGFKILESYNSKSREL 181
           V+D IT R+LISL V E LDM LM+V+TTYLYG ++N+IYMKIPEGFK+LE+ N+K R +
Sbjct: 228 VIDAITFRFLISLAVSEGLDMRLMNVITTYLYGSIDNDIYMKIPEGFKLLEANNTKPRSI 287

Query: 182 YSIKLQRSLYGLKQSGRMWYNRLSEYLLKE 212
           YSIKLQ+SLYGLKQSGRMWYNRLSEYLLKE
Sbjct: 288 YSIKLQQSLYGLKQSGRMWYNRLSEYLLKE 317

[Close]

BLAST of CSPI03G25060 vs. TrEMBL
Match: Q949J4_SOLLC (Putative copia-like polyprotein OS=Solanum lycopersicum PE=4 SV=1)

HSP 1 Score: 303.9 bits (777), Expect = 1.9e-79
Identity = 161/260 (61.92%), Postives = 192/260 (73.85%), Query Frame = 1


Query: 2   TGKRWNRTDVVVDNIFVYNVAHNITHENEDSEPKSVEECCNRKDWPKWKEVIQAKLNSLS 61
           T K WNR +VV+DNIF YNVA  I  ++EDSEPKSV EC  R DWPKWK+ IQA+L SL 
Sbjct: 336 TRKFWNRNNVVIDNIFAYNVAIEIMQQDEDSEPKSVHECRQRNDWPKWKDAIQAELASLE 395

Query: 62  KREVCGPVVHTPKGVKPMGFKWVFVHKRNENNEVTRYKARLVAQGFSQRRGIDYEETYSH 121
           KRE  GP++ TP+G+KP+G+KW+FV KRNE  EV RYKARLVAQGFSQR GIDY ETYS 
Sbjct: 396 KRESFGPIIRTPEGIKPVGYKWIFVRKRNEKGEVMRYKARLVAQGFSQRPGIDYMETYSP 455

Query: 122 VVDVITLRYLISLTVCEKLDMHLMDVVTTYLYGFLENEIYMKIPEGFKILESYNSKSREL 181
           VVD IT RYLI+L V EKL+M LMDVVT YLYG L++ I+MKIPE FK+ E+Y   SRE 
Sbjct: 456 VVDAITFRYLINLAVYEKLEMRLMDVVTAYLYGSLDHNIFMKIPEAFKVPETYKD-SRET 515

Query: 182 YSIKLQRSLYGLKQSGRMWYNRLSEYLLKEDVKRDIFRPQEDNEELLGSEML-------D 241
            SIKLQ+SLYGLKQSGRMWYNRLSEYLLK+  K D   P    +  LGSE +       D
Sbjct: 516 CSIKLQKSLYGLKQSGRMWYNRLSEYLLKKGYKNDSICPCIFIKR-LGSEFVIIAVYVDD 575

Query: 242 IYMIHIKQDLKQVI-CLHVE 254
           + +I  +++L + + CL  E
Sbjct: 576 LNIIGTRKELLEAVECLKRE 593

[Close]

BLAST of CSPI03G25060 vs. TrEMBL
Match: A5C3N6_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_003488 PE=4 SV=1)

HSP 1 Score: 302.8 bits (774), Expect = 4.2e-79
Identity = 144/210 (68.57%), Postives = 174/210 (82.86%), Query Frame = 1


Query: 2   TGKRWNRTDVVVDNIFVYNVAHNITHENEDSEPKSVEECCNRKDWPKWKEVIQAKLNSLS 61
           TG++W+R ++V++NIF + VA +I   +ED EP++ EEC +R D PKWKE IQA+LNSL+
Sbjct: 604 TGEKWDRNNIVINNIFAFQVASDIIRNDEDPEPRNXEECRHRNDXPKWKEAIQAELNSLT 663

Query: 62  KREVCGPVVHTPKGVKPMGFKWVFVHKRNENNEVTRYKARLVAQGFSQRRGIDYEETYSH 121
           KREV GPVV TP+ VKP+G+KWVFV KRNENNE+ RYKARLVAQGFS R GIDYE TYS 
Sbjct: 664 KREVFGPVVQTPEDVKPVGYKWVFVRKRNENNEIIRYKARLVAQGFSXRPGIDYEXTYSP 723

Query: 122 VVDVITLRYLISLTVCEKLDMHLMDVVTTYLYGFLENEIYMKIPEGFKILESYNSKSREL 181
           V+D IT R+LISL V E LDM  MDV+T YLYG ++N+IYMKIPEGFK+ ++ N+K R +
Sbjct: 724 VMDAITFRFLISLAVSEGLDMRXMDVITAYLYGSMDNDIYMKIPEGFKLPDANNTKPRSM 783

Query: 182 YSIKLQRSLYGLKQSGRMWYNRLSEYLLKE 212
           YSIKLQRSLYGLKQSG MWYNRLSEYLLKE
Sbjct: 784 YSIKLQRSLYGLKQSGHMWYNRLSEYLLKE 813

[Close]

BLAST of CSPI03G25060 vs. TAIR10
Match: AT4G23160.1 (AT4G23160.1 cysteine-rich RLK (RECEPTOR-like protein kinase) 8)

HSP 1 Score: 102.4 bits (254), Expect = 4.2e-22
Identity = 49/137 (35.77%), Postives = 85/137 (62.04%), Query Frame = 1


Query: 73  PKGVKPMGFKWVFVHKRNENNEVTRYKARLVAQGFSQRRGIDYEETYSHVVDVITLRYLI 132
           P   KP+G KWV+  K N +  + RYKARLVA+G++Q+ GID+ ET+S V  + +++ ++
Sbjct: 121 PPNKKPIGCKWVYKIKYNSDGTIERYKARLVAKGYTQQEGIDFIETFSPVCKLTSVKLIL 180

Query: 133 SLTVCEKLDMHLMDVVTTYLYGFLENEIYMKIPEGFKILESYNSKSRELYSIKLQRSLYG 192
           +++      +H +D+   +L G L+ EIYMK+P G+   +  +     +  +K  +S+YG
Sbjct: 181 AISAIYNFTLHQLDISNAFLNGDLDEEIYMKLPPGYAARQGDSLPPNAVCYLK--KSIYG 240

Query: 193 LKQSGRMWYNRLSEYLL 210
           LKQ+ R W+ + S  L+
Sbjct: 241 LKQASRQWFLKFSVTLI 255

[Close]

BLAST of CSPI03G25060 vs. TAIR10
Match: ATMG00820.1 (ATMG00820.1 Reverse transcriptase (RNA-dependent DNA polymerase))

HSP 1 Score: 64.3 bits (155), Expect = 1.3e-10
Identity = 44/110 (40.00%), Postives = 64/110 (58.18%), Query Frame = 1


Query: 33  EPKSVEECCNRKDWPKWKEVIQAKLNSLSKREVCGPVVHTPKGVKPMGFKWVFVHKRNEN 92
           EPKSV      KD P W + +Q +L++LS+ +    +V  P     +G KWVF  K + +
Sbjct: 27  EPKSVIFAL--KD-PGWCQAMQEELDALSRNKTW-ILVPPPVNQNILGCKWVFKTKLHSD 86

Query: 93  NEVTRYKARLVAQGFSQRRGIDYEETYSHVVDVITLRYLISLTVCEKLDM 143
             + R KARLVA+GF Q  GI + ETYS VV   T+R +  L V ++L++
Sbjct: 87  GTLDRLKARLVAKGFHQEEGIYFVETYSPVVRTATIRTI--LNVAQQLEV 130

[Close]

BLAST of CSPI03G25060 vs. NCBI nr
Match: gi|147766872|emb|CAN60947.1| (hypothetical protein VITISV_015758 [Vitis vinifera])

HSP 1 Score: 317.4 bits (812), Expect = 2.4e-83
Identity = 150/210 (71.43%), Postives = 180/210 (85.71%), Query Frame = 1


Query: 2    TGKRWNRTDVVVDNIFVYNVAHNITHENEDSEPKSVEECCNRKDWPKWKEVIQAKLNSLS 61
            TG++W+R ++V++NIF + VA +I   +ED EP++VEEC +R DWPKWKE IQA+LNSL+
Sbjct: 803  TGEKWDRNNIVINNIFAFQVASDIIRNDEDPEPRNVEECRHRNDWPKWKEXIQAELNSLT 862

Query: 62   KREVCGPVVHTPKGVKPMGFKWVFVHKRNENNEVTRYKARLVAQGFSQRRGIDYEETYSH 121
            KREV GPVV TP+ VKP+G+KWVFV KRNENNE+ RYKARLVAQGFSQR GIDYEETYS 
Sbjct: 863  KREVFGPVVQTPEDVKPVGYKWVFVRKRNENNEIIRYKARLVAQGFSQRPGIDYEETYSP 922

Query: 122  VVDVITLRYLISLTVCEKLDMHLMDVVTTYLYGFLENEIYMKIPEGFKILESYNSKSREL 181
            V+D IT R+LISL V E LDM LMDV+T YLYG ++N+IYMKIPEGFK+ ++ N+K R +
Sbjct: 923  VMDAITFRFLISLAVSEGLDMRLMDVITAYLYGSMDNDIYMKIPEGFKLPDANNTKPRSM 982

Query: 182  YSIKLQRSLYGLKQSGRMWYNRLSEYLLKE 212
            YSIKLQRSLYGLKQSGRMWYNRLSEYLLKE
Sbjct: 983  YSIKLQRSLYGLKQSGRMWYNRLSEYLLKE 1012

[Close]

BLAST of CSPI03G25060 vs. NCBI nr
Match: gi|147852133|emb|CAN80162.1| (hypothetical protein VITISV_038102 [Vitis vinifera])

HSP 1 Score: 312.0 bits (798), Expect = 9.9e-82
Identity = 147/210 (70.00%), Postives = 178/210 (84.76%), Query Frame = 1


Query: 2   TGKRWNRTDVVVDNIFVYNVAHNITHENEDSEPKSVEECCNRKDWPKWKEVIQAKLNSLS 61
           TG++W+R +++++NIF + VA +I    ED EP++VEEC +R DWPKWKE IQA+LNSL+
Sbjct: 135 TGEKWDRNNIIINNIFAFQVASDIIRNXEDPEPRNVEECRHRNDWPKWKEAIQAELNSLT 194

Query: 62  KREVCGPVVHTPKGVKPMGFKWVFVHKRNENNEVTRYKARLVAQGFSQRRGIDYEETYSH 121
           KREV GP+V TP+ VKP+G+KWVFV K NENNE+ RYKARLVAQGFSQR GIDYEETYS 
Sbjct: 195 KREVFGPIVQTPEDVKPVGYKWVFVRKPNENNEIIRYKARLVAQGFSQRPGIDYEETYSP 254

Query: 122 VVDVITLRYLISLTVCEKLDMHLMDVVTTYLYGFLENEIYMKIPEGFKILESYNSKSREL 181
           ++D IT R+LISL V E LDM LMDV+TTYLYG ++N+IYMKIPEGFK+ E+ N+K R +
Sbjct: 255 IMDAITFRFLISLAVLEGLDMCLMDVITTYLYGSMDNDIYMKIPEGFKLPEANNTKPRSM 314

Query: 182 YSIKLQRSLYGLKQSGRMWYNRLSEYLLKE 212
           YSIKLQRSLYGLKQSGRMWYN LSEYLLKE
Sbjct: 315 YSIKLQRSLYGLKQSGRMWYNCLSEYLLKE 344

[Close]

BLAST of CSPI03G25060 vs. NCBI nr
Match: gi|147820682|emb|CAN69821.1| (hypothetical protein VITISV_030674 [Vitis vinifera])

HSP 1 Score: 310.8 bits (795), Expect = 2.2e-81
Identity = 146/210 (69.52%), Postives = 180/210 (85.71%), Query Frame = 1


Query: 2   TGKRWNRTDVVVDNIFVYNVAHNITHENEDSEPKSVEECCNRKDWPKWKEVIQAKLNSLS 61
           TG++W+  ++V++NIF + VA +I    ED +P+SVEEC +R DWPKWKEVIQA+LNSL+
Sbjct: 108 TGEKWDWNNIVINNIFAFQVASDIIRNYEDLKPRSVEECRHRNDWPKWKEVIQAELNSLT 167

Query: 62  KREVCGPVVHTPKGVKPMGFKWVFVHKRNENNEVTRYKARLVAQGFSQRRGIDYEETYSH 121
           KRE+ GP+V TP+ VKP+G+KWVFV K +ENNE+ RYKARLVAQGFSQR G+DYEETYS 
Sbjct: 168 KREIFGPIVKTPEDVKPIGYKWVFVRKCDENNEIIRYKARLVAQGFSQRPGVDYEETYSP 227

Query: 122 VVDVITLRYLISLTVCEKLDMHLMDVVTTYLYGFLENEIYMKIPEGFKILESYNSKSREL 181
           V+D IT R+LISL V E LDM LM+V+TTYLYG ++N+IYMKIPEGFK+LE+ N+K R +
Sbjct: 228 VIDAITFRFLISLAVSEGLDMRLMNVITTYLYGSIDNDIYMKIPEGFKLLEANNTKPRSI 287

Query: 182 YSIKLQRSLYGLKQSGRMWYNRLSEYLLKE 212
           YSIKLQ+SLYGLKQSGRMWYNRLSEYLLKE
Sbjct: 288 YSIKLQQSLYGLKQSGRMWYNRLSEYLLKE 317

[Close]

BLAST of CSPI03G25060 vs. NCBI nr
Match: gi|15144516|gb|AAK84483.1| (putative copia-like polyprotein [Solanum lycopersicum])

HSP 1 Score: 303.9 bits (777), Expect = 2.7e-79
Identity = 161/260 (61.92%), Postives = 192/260 (73.85%), Query Frame = 1


Query: 2   TGKRWNRTDVVVDNIFVYNVAHNITHENEDSEPKSVEECCNRKDWPKWKEVIQAKLNSLS 61
           T K WNR +VV+DNIF YNVA  I  ++EDSEPKSV EC  R DWPKWK+ IQA+L SL 
Sbjct: 336 TRKFWNRNNVVIDNIFAYNVAIEIMQQDEDSEPKSVHECRQRNDWPKWKDAIQAELASLE 395

Query: 62  KREVCGPVVHTPKGVKPMGFKWVFVHKRNENNEVTRYKARLVAQGFSQRRGIDYEETYSH 121
           KRE  GP++ TP+G+KP+G+KW+FV KRNE  EV RYKARLVAQGFSQR GIDY ETYS 
Sbjct: 396 KRESFGPIIRTPEGIKPVGYKWIFVRKRNEKGEVMRYKARLVAQGFSQRPGIDYMETYSP 455

Query: 122 VVDVITLRYLISLTVCEKLDMHLMDVVTTYLYGFLENEIYMKIPEGFKILESYNSKSREL 181
           VVD IT RYLI+L V EKL+M LMDVVT YLYG L++ I+MKIPE FK+ E+Y   SRE 
Sbjct: 456 VVDAITFRYLINLAVYEKLEMRLMDVVTAYLYGSLDHNIFMKIPEAFKVPETYKD-SRET 515

Query: 182 YSIKLQRSLYGLKQSGRMWYNRLSEYLLKEDVKRDIFRPQEDNEELLGSEML-------D 241
            SIKLQ+SLYGLKQSGRMWYNRLSEYLLK+  K D   P    +  LGSE +       D
Sbjct: 516 CSIKLQKSLYGLKQSGRMWYNRLSEYLLKKGYKNDSICPCIFIKR-LGSEFVIIAVYVDD 575

Query: 242 IYMIHIKQDLKQVI-CLHVE 254
           + +I  +++L + + CL  E
Sbjct: 576 LNIIGTRKELLEAVECLKRE 593

[Close]

BLAST of CSPI03G25060 vs. NCBI nr
Match: gi|147816183|emb|CAN68598.1| (hypothetical protein VITISV_003488 [Vitis vinifera])

HSP 1 Score: 302.8 bits (774), Expect = 6.0e-79
Identity = 144/210 (68.57%), Postives = 174/210 (82.86%), Query Frame = 1


Query: 2   TGKRWNRTDVVVDNIFVYNVAHNITHENEDSEPKSVEECCNRKDWPKWKEVIQAKLNSLS 61
           TG++W+R ++V++NIF + VA +I   +ED EP++ EEC +R D PKWKE IQA+LNSL+
Sbjct: 604 TGEKWDRNNIVINNIFAFQVASDIIRNDEDPEPRNXEECRHRNDXPKWKEAIQAELNSLT 663

Query: 62  KREVCGPVVHTPKGVKPMGFKWVFVHKRNENNEVTRYKARLVAQGFSQRRGIDYEETYSH 121
           KREV GPVV TP+ VKP+G+KWVFV KRNENNE+ RYKARLVAQGFS R GIDYE TYS 
Sbjct: 664 KREVFGPVVQTPEDVKPVGYKWVFVRKRNENNEIIRYKARLVAQGFSXRPGIDYEXTYSP 723

Query: 122 VVDVITLRYLISLTVCEKLDMHLMDVVTTYLYGFLENEIYMKIPEGFKILESYNSKSREL 181
           V+D IT R+LISL V E LDM  MDV+T YLYG ++N+IYMKIPEGFK+ ++ N+K R +
Sbjct: 724 VMDAITFRFLISLAVSEGLDMRXMDVITAYLYGSMDNDIYMKIPEGFKLPDANNTKPRSM 783

Query: 182 YSIKLQRSLYGLKQSGRMWYNRLSEYLLKE 212
           YSIKLQRSLYGLKQSG MWYNRLSEYLLKE
Sbjct: 784 YSIKLQRSLYGLKQSGHMWYNRLSEYLLKE 813

The following BLAST results are available for this feature:

BLAST of CSPI03G25060 vs. Swiss-Prot


Match Name	E-value	Identity	Description
POLX_TOBAC	1.7e-28	37.22	Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum...	[more]
COPIA_DROME	6.5e-25	39.33	Copia protein OS=Drosophila melanogaster GN=GIP PE=1 SV=3	[more]
M820_ARATH	2.3e-09	40.00	Uncharacterized mitochondrial protein AtMg00820 OS=Arabidopsis thaliana GN=AtMg0...	[more]
YO11B_YEAST	1.4e-06	30.70	Transposon Ty1-OL Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 2...	[more]
YA11B_YEAST	1.4e-06	31.09	Truncated transposon Ty1-A Gag-Pol polyprotein OS=Saccharomyces cerevisiae (stra...	[more]

BLAST of CSPI03G25060 vs. TrEMBL


Match Name	E-value	Identity	Description
A5B4M9_VITVI	1.6e-83	71.43	Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_015758 PE=4 SV=1	[more]
A5AK32_VITVI	6.9e-82	70.00	Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_038102 PE=4 SV=1	[more]
A5B8K5_VITVI	1.5e-81	69.52	Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_030674 PE=4 SV=1	[more]
Q949J4_SOLLC	1.9e-79	61.92	Putative copia-like polyprotein OS=Solanum lycopersicum PE=4 SV=1	[more]
A5C3N6_VITVI	4.2e-79	68.57	Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_003488 PE=4 SV=1	[more]

BLAST of CSPI03G25060 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G23160.1	4.2e-22	35.77	cysteine-rich RLK (RECEPTOR-like protein kinase) 8	[more]
ATMG00820.1	1.3e-10	40.00	ATMG00820.1 Reverse transcriptase (RNA-dependent DNA polymerase)	[more]

BLAST of CSPI03G25060 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|147766872\|emb\|CAN60947.1\|	2.4e-83	71.43	hypothetical protein VITISV_015758 [Vitis vinifera]	[more]
gi\|147852133\|emb\|CAN80162.1\|	9.9e-82	70.00	hypothetical protein VITISV_038102 [Vitis vinifera]	[more]
gi\|147820682\|emb\|CAN69821.1\|	2.2e-81	69.52	hypothetical protein VITISV_030674 [Vitis vinifera]	[more]
gi\|15144516\|gb\|AAK84483.1\|	2.7e-79	61.92	putative copia-like polyprotein [Solanum lycopersicum]	[more]
gi\|147816183\|emb\|CAN68598.1\|	6.0e-79	68.57	hypothetical protein VITISV_003488 [Vitis vinifera]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR013103	RVT_2

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0015074	DNA integration
biological_process	GO:0019538	protein metabolic process
biological_process	GO:0044763	single-organism cellular process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0008270	zinc ion binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G25060.1	CSPI03G25060.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013103	Reverse transcriptase, RNA-dependent DNA polymerase	PFAM	PF07727	RVT_2	coord: 69..215 score: 1.9
None	No IPR available	PANTHER	PTHR11439	GAG-POL-RELATED RETROTRANSPOSON	coord: 1..212 score: 5.2
None	No IPR available	PANTHER	PTHR11439:SF180	SUBFAMILY NOT NAMED	coord: 1..212 score: 5.2

Synteny

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CSPI03G25060	Bottle gourd (USVL1VR-Ls)	cpilsiB230
CSPI03G25060	Melon (DHL92) v3.6.1	cpimedB197
CSPI03G25060	Cucumber (Gy14) v2	cgybcpiB110
CSPI03G25060	Wax gourd	cpiwgoB298
CSPI03G25060	Cucumber (Chinese Long) v3	cpicucB144
CSPI03G25060	Cucumber (Gy14) v1	cgycpiB451
CSPI03G25060	Cucumber (Chinese Long) v2	cpicuB120
CSPI03G25060	Melon (DHL92) v3.5.1	cpimeB206
CSPI03G25060	Watermelon (97103) v1	cpiwmB229