CSPI03G21540 (gene) Wild cucumber (PI 183967)

NameCSPI03G21540
Typegene
OrganismCucumis sativus (Wild cucumber (PI 183967))
DescriptionBnaA07g27430D protein
LocationChr3 : 17821420 .. 17822067 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGAAATTTTCCGTTGATAAGAAAAACATCAACCTCATCAAATGGTCAATGGTGCTGTCTCCTATCAACAAAGGTGACCTGGACATAAACAGCGTTCAGAGTACAAATTTCGCTCTACTGAGCAAGTGGACCTGGATATTTTACGAAGAAAAAAATCATCTGTGGAAATGCATAATCATTGCTAAATATGAACAATCATACTTGGGTGAATTTCCAACTAAAAGCAAATATAGCAGCTCCAAAGCCCCTTGGATGTCTATCATTAAAGGAGCTGACTGGGTCCTTCCTCAAATCAAATGGACCATTAAAAGGGGAGACTCCCTCTCATTCTGGCATAGCCGATGGCATGATCTTAGTCCATTCACACAGTCCAACCCAAGATTATTTGCACTGTCTACCAGGAAAGGAGACTCCATTGCAAACATGTGGAATGCAGAAACAGCCGACTGCGACCTCTACCCTCGAAGACCATTAAGAAGTGTTGAGGAAGTACTTTGGGACGATATGAAAGCCTCCCCCCCATTGCCTGATTCTGGATTCGACAATCCTCGCTGGATTTTAAACAACAATGGCAGCTTCACTATGGCTTCTATCAAACTCGCAAGACCTCCAAACAACCAAGATGAGACCAACGAAGATGATGGA

mRNA sequence

ATGGAGAAATTTTCCGTTGATAAGAAAAACATCAACCTCATCAAATGGTCAATGGTGCTGTCTCCTATCAACAAAGGTGACCTGGACATAAACAGCGTTCAGAGTACAAATTTCGCTCTACTGAGCAAGTGGACCTGGATATTTTACGAAGAAAAAAATCATCTGTGGAAATGCATAATCATTGCTAAATATGAACAATCATACTTGGGTGAATTTCCAACTAAAAGCAAATATAGCAGCTCCAAAGCCCCTTGGATGTCTATCATTAAAGGAGCTGACTGGGTCCTTCCTCAAATCAAATGGACCATTAAAAGGGGAGACTCCCTCTCATTCTGGCATAGCCGATGGCATGATCTTAGTCCATTCACACAGTCCAACCCAAGATTATTTGCACTGTCTACCAGGAAAGGAGACTCCATTGCAAACATGTGGAATGCAGAAACAGCCGACTGCGACCTCTACCCTCGAAGACCATTAAGAAGTGTTGAGGAAGTACTTTGGGACGATATGAAAGCCTCCCCCCCATTGCCTGATTCTGGATTCGACAATCCTCGCTGGATTTTAAACAACAATGGCAGCTTCACTATGGCTTCTATCAAACTCGCAAGACCTCCAAACAACCAAGATGAGACCAACGAAGATGATGGA

Coding sequence (CDS)

ATGGAGAAATTTTCCGTTGATAAGAAAAACATCAACCTCATCAAATGGTCAATGGTGCTGTCTCCTATCAACAAAGGTGACCTGGACATAAACAGCGTTCAGAGTACAAATTTCGCTCTACTGAGCAAGTGGACCTGGATATTTTACGAAGAAAAAAATCATCTGTGGAAATGCATAATCATTGCTAAATATGAACAATCATACTTGGGTGAATTTCCAACTAAAAGCAAATATAGCAGCTCCAAAGCCCCTTGGATGTCTATCATTAAAGGAGCTGACTGGGTCCTTCCTCAAATCAAATGGACCATTAAAAGGGGAGACTCCCTCTCATTCTGGCATAGCCGATGGCATGATCTTAGTCCATTCACACAGTCCAACCCAAGATTATTTGCACTGTCTACCAGGAAAGGAGACTCCATTGCAAACATGTGGAATGCAGAAACAGCCGACTGCGACCTCTACCCTCGAAGACCATTAAGAAGTGTTGAGGAAGTACTTTGGGACGATATGAAAGCCTCCCCCCCATTGCCTGATTCTGGATTCGACAATCCTCGCTGGATTTTAAACAACAATGGCAGCTTCACTATGGCTTCTATCAAACTCGCAAGACCTCCAAACAACCAAGATGAGACCAACGAAGATGATGGA
BLAST of CSPI03G21540 vs. Swiss-Prot
Match: RNHX1_ARATH (Putative ribonuclease H protein At1g65750 OS=Arabidopsis thaliana GN=At1g65750 PE=3 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 2.0e-11
Identity = 39/121 (32.23%), Postives = 57/121 (47.11%), Query Frame = 1

Query: 5   SVDKKNINLIKWSMVLSPINKGDLDINSVQSTNFALLSKWTWIFYEEKNHLWKCIIIAKY 64
           + +KK  +L+KWS V SP  +G L + + +S N AL+SK  W   +EKN LW  ++  KY
Sbjct: 78  TAEKKKQHLVKWSKVCSPKKEGGLGVRAAKSMNRALISKVGWRLLQEKNSLWTLVLQKKY 137

Query: 65  EQSYLGEFPTKSKYSSSKAPWMSIIKG-ADWVLPQIKWTIKRGDSLSFWHSRWHDLSPFT 124
               + +        S  + W SI  G  D V   + W    G  + FW  RW    P  
Sbjct: 138 HVGEIRDSRWLIPKGSWSSTWRSIAIGLRDVVSHGVGWIPGDGQQIRFWTDRWVSGKPLL 197

BLAST of CSPI03G21540 vs. TrEMBL
Match: A5AJ71_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_033905 PE=4 SV=1)

HSP 1 Score: 102.1 bits (253), Expect = 8.9e-19
Identity = 45/142 (31.69%), Postives = 73/142 (51.41%), Query Frame = 1

Query: 11  INLIKWSMVLSPINKGDLDINSVQSTNFALLSKWTWIFYEEKNHLWKCIIIAKYEQSYLG 70
           ++L+KW  V +  +KG L +  + + N ALL KW W F  EKN+ W  +I  KY Q   G
Sbjct: 190 VHLVKWDAVCTEKHKGGLGLRRIATLNRALLGKWIWRFACEKNNFWNQVITTKYGQEDYG 249

Query: 71  EFPTKSKYSSSKAPWMSIIKGADWVLPQIKWTIKRGDSLSFWHSRWHDLSPFTQSNPRLF 130
             P K + ++    W  I+K +DW    + + + +G  + FW  RW    P +Q   +LF
Sbjct: 250 WRPKKVRGAAGAGVWKEIMKESDWCWENLAFVVGKGSKIKFWKDRWCTDIPLSQCXNQLF 309

Query: 131 ALSTRKGDSIANMWNAETADCD 153
            L+  +  +I  MW+ ++   D
Sbjct: 310 VLAVHRDATIEEMWDQDSGQGD 331

BLAST of CSPI03G21540 vs. TrEMBL
Match: A5C9R7_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_010990 PE=4 SV=1)

HSP 1 Score: 99.8 bits (247), Expect = 4.4e-18
Identity = 45/142 (31.69%), Postives = 72/142 (50.70%), Query Frame = 1

Query: 11   INLIKWSMVLSPINKGDLDINSVQSTNFALLSKWTWIFYEEKNHLWKCIIIAKYEQSYLG 70
            ++L+KW  V +  +KG L +  + + N ALL KW W F  EKN+ W  +I  KY Q   G
Sbjct: 1205 VHLVKWDAVCTEKHKGGLGLRRIATLNRALLGKWIWRFACEKNNFWNQVITTKYGQEDYG 1264

Query: 71   EFPTKSKYSSSKAPWMSIIKGADWVLPQIKWTIKRGDSLSFWHSRWHDLSPFTQSNPRLF 130
              P K   ++    W  I+K +DW    + + + +G  + FW  RW    P +Q   +LF
Sbjct: 1265 WRPKKVSGAAGVGVWKEIMKESDWCWENLAFIVGKGSKIKFWKDRWCTDIPLSQCFNQLF 1324

Query: 131  ALSTRKGDSIANMWNAETADCD 153
             L+  +  +I  MW+ ++   D
Sbjct: 1325 VLAVHRDATIEEMWDQDSGQGD 1346

BLAST of CSPI03G21540 vs. TrEMBL
Match: A5BXL2_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_023185 PE=4 SV=1)

HSP 1 Score: 99.8 bits (247), Expect = 4.4e-18
Identity = 59/204 (28.92%), Postives = 92/204 (45.10%), Query Frame = 1

Query: 11  INLIKWSMVLSPINKGDLDINSVQSTNFALLSKWTWIFYEEKNHLWKCIIIAKYEQSYLG 70
           ++L+KW  V +  +KG L +  + + N ALL KW W F  EKN+ W  +I  KY Q   G
Sbjct: 722 VHLVKWDAVCTEKHKGGLGLRRIATLNRALLGKWIWRFACEKNNFWNQVITTKYGQEDYG 781

Query: 71  EFPTKSKYSSSKAPWMSIIKGADWVLPQIKWTIKRGDSLSFWHSRWHDLSPFTQSNPRLF 130
             P K +  +    W  I+K  DW    + + + +G  + FW   W   +P +Q   +LF
Sbjct: 782 WRPKKVRGPAGVGVWKEIMKEDDWCWDNLAFRVGKGSKIKFWKDCWCTDTPLSQCFNQLF 841

Query: 131 ALSTRKGDSIANMWNAETADCDL-------YPRRPLRSVEEVLWDDMKASPPLPDSGFDN 190
           AL+  +  +I  MW+ +    D        +    +  V E+L       P L D   D+
Sbjct: 842 ALAVHRDATIEEMWDHDAGQGDWKLVFVRDFNDWEMDMVGELLHTLRGQRPSLED---DS 901

Query: 191 PRWILNNNGSFTM-ASIKLARPPN 207
             W    NG F +  + +L   PN
Sbjct: 902 VVWRQGRNGIFKIKEAYRLLDKPN 922

BLAST of CSPI03G21540 vs. TrEMBL
Match: A5ARU9_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_033107 PE=4 SV=1)

HSP 1 Score: 99.8 bits (247), Expect = 4.4e-18
Identity = 57/191 (29.84%), Postives = 89/191 (46.60%), Query Frame = 1

Query: 11  INLIKWSMVLSPINKGDLDINSVQSTNFALLSKWTWIFYEEKNHLWKCIIIAKYEQSYLG 70
           I+L+KW +V +    G L +  + + N ALL KW W F  E+++LWK +I  KY Q   G
Sbjct: 797 IHLVKWDVVCTEKRNGGLGLRRIATLNRALLGKWIWRFACERDNLWKQVISTKYGQEDYG 856

Query: 71  EFPTKSKYSSSKAPWMSIIKGADWVLPQIKWTIKRGDSLSFWHSRWHDLSPFTQSNPRLF 130
               K   ++    W  I+K +DW    + + + +G  + FW  RW   +P +Q    LF
Sbjct: 857 WRAKKVSGAAGVGVWKEIMKESDWCWENLSFLVGKGSKIKFWKDRWCTDTPLSQCFNHLF 916

Query: 131 ALSTRKGDSIANMWNAET--ADCDLYPRRPLRSVEEVLWDDMKAS-----PPLPDSGFDN 190
            L+  +  SI  MW+  +   D +L   R     E  +  D+  +     P L D   D+
Sbjct: 917 VLAVHRDASIEEMWDHHSGQGDWNLIFERDFNDWELDMVGDLLRTLRGHRPSLED---DS 976

Query: 191 PRWILNNNGSF 195
            +W     GSF
Sbjct: 977 IKWRQGRKGSF 984

BLAST of CSPI03G21540 vs. TrEMBL
Match: A5BAM5_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_025197 PE=4 SV=1)

HSP 1 Score: 97.1 bits (240), Expect = 2.9e-17
Identity = 48/145 (33.10%), Postives = 76/145 (52.41%), Query Frame = 1

Query: 1   MEKFSVDKKNINLIKWSMVLSPINKGDLDINSVQSTNFALLSKWTWIFYEEKNHLWKCII 60
           M  F + K+ ++L+KW +V +   KG L I  +   N ALL +  WIF  EK +LWK +I
Sbjct: 158 MSLFRMPKRKVHLVKWEVVSADKEKGGLSIRKLAFLNKALLGR--WIFAFEKENLWKKVI 217

Query: 61  IAKYEQSYLGEFPTKSKYSSSKAPWMSIIKGADWVLPQIKWTIKRGDSLSFWHSRWHDLS 120
             KY Q  LG    K+  +     W  I+K  +W    I +T+  G  + FW   W  L+
Sbjct: 218 SVKYGQEGLGWRTNKANGTFRVGVWKEILKETNWCWENIGFTVGNGTKIRFWTDHWCGLA 277

Query: 121 PFTQSNPRLFALSTRKGDSIANMWN 146
             +QS P+L+AL+ ++   +  +W+
Sbjct: 278 ALSQSFPQLYALAVQRNARVDEVWD 300

BLAST of CSPI03G21540 vs. NCBI nr
Match: gi|659122972|ref|XP_008461420.1| (PREDICTED: putative ribonuclease H protein At1g65750 [Cucumis melo])

HSP 1 Score: 194.1 bits (492), Expect = 2.5e-46
Identity = 89/195 (45.64%), Postives = 123/195 (63.08%), Query Frame = 1

Query: 10  NINLIKWSMVLSPINKGDLDINSVQSTNFALLSKWTWIFYEEKNHLWKCIIIAKYEQSYL 69
           NI+LI+W+ ++SP  KG L I+SV STNFALL KW W F  EK+ LWK +II+KY++  +
Sbjct: 31  NISLIRWNQIVSPKEKGGLGIHSVNSTNFALLCKWLWKFLTEKDPLWKRLIISKYDKEKM 90

Query: 70  GEFPTKSKYSSSKAPWMSIIKGADWVLPQIKWTIKRGDSLSFWHSRWHDLSPFTQSNPRL 129
           G FP+  K+SS+ +PW ++ +   W    I W +  GD +SFW   W+  +P + + PRL
Sbjct: 91  GSFPSHGKFSSNNSPWKAVTECISWFYNNISWKVNDGDDISFWLDNWNGNAPLSLAVPRL 150

Query: 130 FALSTRKGDSIANMWNAETADCDLYPRRPLRSVEEVLWDDMKAS--PPLPDSGFDNPRWI 189
           FALST K  ++   WN  + D  L+  RPLR  EE LW ++KAS   PLP+ G   P W 
Sbjct: 151 FALSTNKKGTVKEFWNPSSNDWHLHINRPLRDHEENLWHNIKASLPTPLPNRGHPKPLWK 210

Query: 190 LNNNGSFTMASIKLA 203
           LN+N  F  AS+K A
Sbjct: 211 LNSNNIFDTASVKRA 225

BLAST of CSPI03G21540 vs. NCBI nr
Match: gi|659068893|ref|XP_008446987.1| (PREDICTED: uncharacterized protein LOC103489530 [Cucumis melo])

HSP 1 Score: 142.9 bits (359), Expect = 6.6e-31
Identity = 71/191 (37.17%), Postives = 99/191 (51.83%), Query Frame = 1

Query: 13  LIKWSMVLSPINKGDLDINSVQSTNFALLSKWTWIFYEEKNHLWKCIIIAKYEQSYLGEF 72
           LI W+ V     +G L I+ +  TN ALLSKW W +  E N LW+ +I  KY+ +Y G+ 
Sbjct: 281 LINWTKVFKSKEEGGLGISRLHVTNKALLSKWLWCYLSEPNALWRRLIQCKYKGNYPGDI 340

Query: 73  PTKSKYSSSKAPWMSIIKGADWVLPQIKWTIKRGDSLSFWHSRWHDLSPFTQSNPRLFAL 132
           P+    S+SKAPW SII   DW      W +  GD +SFW+S W      + + PRLFAL
Sbjct: 341 PSNISSSTSKAPWRSIIDNIDWFKSNQSWELNNGDQISFWYSNWSQEGHLSTAYPRLFAL 400

Query: 133 STRKGDSIANMWNAETADCDLYPRRPLRSVEEVLWDDMKASPPLPDSG--FDNPRWILNN 192
           +  K  S+ + WN      ++  RR L   E   W  +    P P S      P W  ++
Sbjct: 401 TLGKEISVKDAWNTFDNQWNIIFRRELNDRERCKWAKILEILPTPRSNRRSSKPTWTPDS 460

Query: 193 NGSFTMASIKL 202
           N SF++AS K+
Sbjct: 461 NNSFSIASAKV 471

BLAST of CSPI03G21540 vs. NCBI nr
Match: gi|659130833|ref|XP_008465376.1| (PREDICTED: putative ribonuclease H protein At1g65750 [Cucumis melo])

HSP 1 Score: 139.8 bits (351), Expect = 5.6e-30
Identity = 64/159 (40.25%), Postives = 92/159 (57.86%), Query Frame = 1

Query: 5   SVDKKNINLIKWSMVLSPINKGDLDINSVQSTNFALLSKWTWIFYEEKNHLWKCIIIAKY 64
           S DK+N +L+ W +  SP   G L I+ ++ TN ALL KW W ++ E N LWK  I AKY
Sbjct: 288 SEDKQNAHLVNWRICTSPKELGGLGISKLKDTNQALLCKWLWRYHNESNSLWKKCIDAKY 347

Query: 65  EQSYLGEFPTKSKYSSSKAPWMSIIKGADWVLPQIKWTIKRGDSLSFWHSRWHDLSPFTQ 124
            +++ G+ P   +  S K+PW +I K  DW   +I W    G SLSFWHS+W +  P + 
Sbjct: 348 TKNHQGDIPIVGRNCSVKSPWNAIKKWKDWFESKISWIPNDGSSLSFWHSKWQNNIPLSH 407

Query: 125 SNPRLFALSTRKGDSIANMWNAETADCDLYPRRPLRSVE 164
             PRL+ALS  +  ++  +W+  T   ++ PRRPL   E
Sbjct: 408 QIPRLYALSNLQSATVKEVWDQSTDGWNINPRRPLNERE 446

BLAST of CSPI03G21540 vs. NCBI nr
Match: gi|659131345|ref|XP_008465637.1| (PREDICTED: putative ribonuclease H protein At1g65750, partial [Cucumis melo])

HSP 1 Score: 137.1 bits (344), Expect = 3.6e-29
Identity = 70/192 (36.46%), Postives = 100/192 (52.08%), Query Frame = 1

Query: 12  NLIKWSMVLSPINKGDLDINSVQSTNFALLSKWTWIFYEEKNHLWKCIIIAKYEQSYLGE 71
           +LI W+ V     +G L I  +  TN ALL+KW W +  E   LW+ +I  KY+ +Y G+
Sbjct: 47  HLINWTKVSKSKEEGGLGILRLHVTNKALLTKWLWRYLSEPKALWRSLIECKYKGNYPGD 106

Query: 72  FPTKSKYSSSKAPWMSIIKGADWVLPQIKWTIKRGDSLSFWHSRWHDLSPFTQSNPRLFA 131
            P+    S+SKAPW SII   DW      W +  GD +SFW+S W      + + PRLFA
Sbjct: 107 IPSNISSSTSKAPWRSIIDNIDWFKSNQSWELNNGDQISFWYSNWSLEGRLSTAYPRLFA 166

Query: 132 LSTRKGDSIANMWNAETADCDLYPRRPLRSVEEVLWDDMKASPPLPDS--GFDNPRWILN 191
           L+  K  S+ + WN      ++  RR L   E   W  +    P P S  G   P WI +
Sbjct: 167 LTLDKEISVKDAWNTFDNKWNIIFRRELNDRERSKWAKILEILPSPRSNRGSSKPTWIPD 226

Query: 192 NNGSFTMASIKL 202
           ++ SF++AS K+
Sbjct: 227 SDNSFSIASAKV 238

BLAST of CSPI03G21540 vs. NCBI nr
Match: gi|659086627|ref|XP_008444035.1| (PREDICTED: putative ribonuclease H protein At1g65750 [Cucumis melo])

HSP 1 Score: 135.6 bits (340), Expect = 1.0e-28
Identity = 71/193 (36.79%), Postives = 100/193 (51.81%), Query Frame = 1

Query: 12  NLIKWSMVLSPINKGDLDINSVQSTNFALLSKWTWIFYEEKNHLWKCIIIAKYEQSYLGE 71
           +LI W+ V     +G L I+ +  TN ALL+KW W +  E N LW+ +I  KY+ +Y G+
Sbjct: 99  HLINWTKVSKSKEEGGLGISRLHVTNKALLTKWLWRYLSEPNALWRSLIQCKYKGNYPGD 158

Query: 72  FPTKSKYSSSKAPWMSIIKG-ADWVLPQIKWTIKRGDSLSFWHSRWHDLSPFTQSNPRLF 131
            P+    S+SKAPW SII    DW      W +  GD +SFW+S W      + + PRLF
Sbjct: 159 IPSNISSSTSKAPWRSIIDNIIDWFKSNQSWKLNNGDQISFWYSNWSLEGRLSTAYPRLF 218

Query: 132 ALSTRKGDSIANMWNAETADCDLYPRRPLRSVEEVLWDDMKASPPLPDS--GFDNPRWIL 191
           AL+  K  S+ + WN      ++  RR L   E   W  +    P P S  G   P W  
Sbjct: 219 ALTLDKEISVKDAWNTFDNKWNIIFRRELNDRERSKWAKILEILPSPRSNRGSSKPTWTP 278

Query: 192 NNNGSFTMASIKL 202
           ++N SF+ AS K+
Sbjct: 279 DSNNSFSTASAKV 291

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
RNHX1_ARATH2.0e-1132.23Putative ribonuclease H protein At1g65750 OS=Arabidopsis thaliana GN=At1g65750 P... [more]
Match NameE-valueIdentityDescription
A5AJ71_VITVI8.9e-1931.69Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_033905 PE=4 SV=1[more]
A5C9R7_VITVI4.4e-1831.69Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_010990 PE=4 SV=1[more]
A5BXL2_VITVI4.4e-1828.92Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_023185 PE=4 SV=1[more]
A5ARU9_VITVI4.4e-1829.84Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_033107 PE=4 SV=1[more]
A5BAM5_VITVI2.9e-1733.10Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_025197 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
gi|659122972|ref|XP_008461420.1|2.5e-4645.64PREDICTED: putative ribonuclease H protein At1g65750 [Cucumis melo][more]
gi|659068893|ref|XP_008446987.1|6.6e-3137.17PREDICTED: uncharacterized protein LOC103489530 [Cucumis melo][more]
gi|659130833|ref|XP_008465376.1|5.6e-3040.25PREDICTED: putative ribonuclease H protein At1g65750 [Cucumis melo][more]
gi|659131345|ref|XP_008465637.1|3.6e-2936.46PREDICTED: putative ribonuclease H protein At1g65750, partial [Cucumis melo][more]
gi|659086627|ref|XP_008444035.1|1.0e-2836.79PREDICTED: putative ribonuclease H protein At1g65750 [Cucumis melo][more]
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0003674 molecular_function

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI03G21540.1CSPI03G21540.1mRNA


The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None