CSPI03G19650 (gene) Wild cucumber (PI 183967) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CSPI03G19650
Type	gene
Organism	Cucumis sativus (Wild cucumber (PI 183967))
Description	Calcium-binding EF hand family protein
Location	Chr3 : 15397985 .. 15401800 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AGAAAAACAAAAAGGAAATTATCCTAAATTGAAAATAATTTTTAAAAAGTTATTTTATTAACGTCTAAGAGTAAATATTTTTAATATACAAAACCTCTGAGCCTCAGAATCGATTCCACCGTTGCCTATTATTGCAAAGCTTAGTGAACGAACGGTACTCCGTTCATCTTCTTCCCCAGCCCTCACCTCCACCCATTTTCCACTTATTTTTTCTCAAATGTCGCCTTCTCTACGTATCCCTCCTTTTCCCCATCTAATCTCATCTCAATCTCTTCCGCCGCCAGAAAAATGGGGAAGGCGGTGGTATATGTTCTTACAGCCACCGTCTTCGCTCTCTTCTTCCTAATTTCTCCCTCCAATTTCCACAATCGGAGCCATCAACAGGCAACTCGTCGTCTAGGATTCAAATTCCCAAACCCTACTTTCGATCCTCTTGTTACGGAAATGGAGAGATTAGCAGCAGAGGAACGTGGTGAAAATGCGATTGGAGTGGACAATCAAAACCATAAGATCATCGATTCTTATAGAAATTACTACGATGAAGGGAGATTGAACATTTCGTTGAGGTTACTTGTGTTGTTTCCTTTGCTGGATAATTCGCCTAAAGATGGGGTTATCAGTTACGAGGAGTTGAGTGATTGGATTAATGGGCAAGCTATTGAAAGATTGAATTATAGAACGACGAAACAATTGGAGTTCTACGATAAGAATGGAGATGACGCCATTTCATTCCATGAATATCTGCCGCAGTTCACTGAGGAAGATATTGGTACGTGTTTCTTCATTTCTTTGATTATGATCGAAATTAGCTAAAATATTTTTCTGGTGATTTTAGGCTTACTTTCTTCTCATTGTTGTGTTTCATAACGTTACTCTTTCTTGGTTTTTTTGTTTCAATTTAGTTAGTTTTTTTTAAATGTTAAACTTTTAGAGGTTTGAATTTTGTTTCTATTTATACTTAGTTTTCGTAACCTTTTTAGTCTTGATTTTGTGTCTATTTATTATTTTAAATAAATAATTTCAAAGAATTATAGTTAATTAAACATCACCGATTTTCTATCTCTGTTTAGCAAAAAAGTGACGAAAAAGTAGTAGAGGTTTGTGAGGTGGGTTGAAATACTTTTAGATTCAACTCAATTGTTTAGTAAATTTATCTGATTCAAATAATTTGAATTAAAAATTTAACCCAACACAATTTTGGAATACTTGGATTTGGTTATTTTCGGTTGGTTGGATCATTTAAAATTTTTATTCTAAAAAGAGTAAATTTCTCGGGTTCAAATAATTTGAATTAAAAATTTAACCCAACACACAAACTTAGAATACTTAGATTTGATTATTTCCCGTTGGTTGTTGGATCATTTAAAACTTTTGTTCTAAAAAAGTAAAGTGTGATTTGAAAACTCAATTCTAATTTATATAATACAACTTTTTTCGAAAGCACAGAAAAAACTACTTTTAAGATTTGTTGGAGGAGAATAAATTATCAAATAAACAATGAAATTGAATAAAACAAAAATAAATATTAAAAATATAATTGTATATAATTAAGAGGGAAAGGTTTTACATTACACTTGGCTGTAAAATTGTATAAAGAGGGTTTTGATGAACTGTTGTGTAACCAAAAACGTACGTATTTTGCCTAATTAAAATATAAAAATGTAAGCAACTACTGTTGTGTAAAAATGTTTGACTGAATAATTGATATAGGAATAATGTGAGAGTGAGGAAAACACTACATTAGAATCCGTACAAAGGAACAACATTTAGAGATGTAAAACCTTTATCCAACATTGTCGAATGGGAACTTTGAAATATATAGGAAAGTCATAGAGTATATATGGTAACTTTTCAAAAAAAAAGAAAAGAGAAAATTGGATATCACTTTTAGAAAAAGTAGAAGGTAATTTTTGTAAGTTAGGCTATTATTTTTCACATTGTGGGACTGAGACAGAAATAACATAGTGAATATATTATATTTTTGAATGAAACAGCAAGAAATGAAACGGGATATGGAGAAGCTGGATGGTGGAGAAAGCAATTCACAAATGCGGATGTTGACAACAATGGCTTACTATATTTTGATGAACTTAAAGAGTAATGTCCCATCTTTCTCTACCATAAACAATAAGGTCTACTTCACTATTTTTAACCTTAATTACTATACATAAGAATATGTATATGTATACAAACTTATTACAATATTATCATAAACATTGCCTAGTGTAATTTTAGAGAAAAATCAATAACTTGGACATGTGTGCTGATGGTCACCTAGAAAGGTGTGGCAATGTTCAAGTTTCAATTTATACTATTTCAAATGTAGTATTGTAATATTTTAAATGATTCACAATTTTTTTAAGGTTAAAATACCATTTTATGGTATACTTTAGTTTATTTAATTTTAGTCTCCAACTTTCAAGAGTAATTTTGCTATAAGGTTTTTAAAATGCATTCACTTATTGTATTTCCTTACATGAAAATTATTGTAATTATTTAATCAATTCTAGTAAAAACTAACTTTGAAATACTACAATTAAAATTTTATTAGAAGCACAGACTTAAACTGAATCTAACCGAGACCAAAAAAAGTTTTTAATTCTTTTTTTAAAAGGGTATGAGATATTTGTATCTTGCAGTTTCCTTCACCCAGAAGATAGCAGCAATTACCGAATACAAAACTGGCTGTTGGCACAGAAAATGAAGTAACTTCTTCCGCTATACTTGAATCCATTAACCTTTATCATCTTAAAACCAAACCATCATCTTCCTGTTTAATTCATTCTTATGCAGGCGCATGGACCATGACAAAGATGGAAAGCTCAATTTTGATGAATTCCTTCACCATACATATGATATATACAAGAACTATATTGAATTTGAAACTCAAGGCGAAGATGTACCAAGCGCTGAAGAGAAGTTTGATGAGCTTGATCTCGACGAGGATGAGTATGGCAATGCATTTGTTTGGAGAGTTATTTTTAGGGAAATTTTATATATATCAAAATACAAAAATTTCATCAACTCATCGAAATGACTTTTTTTTCTATTTACTATACTATTTCAGTCTTTATAATTTTGATGTTGTTTCATTTTAGTTTCTATACTATCAATATGTTCGTTTTAGTTCACACGCTTTTAACTTTGGTTCCCATTTTGGTTCTTATATCATTTTTAAAGAACCAAAATTATCTATCTTTTTTAAAGTTCAAGGACCAAACTGAATAAAAAACAAAAGTATAGGGACTAAAATAGTATTTAAACTTGTTTATGTGTATTTGAAAATATTTGTAACGGTTTTGTCCGTTCTCTCTCACTTTTGTTGTTAATTTATTTGTAATATCAAACATTCTTGTGCAGAGTATTGTCAACGGAAGAATTAAGACCATTGTTCCAGTATCTACATCCTGGAGAAGTGTCTTATGCTCAACATTACACAAGTCATTTGATTAATGAGGTACGTAATTAGTAGTTGAATTGAAGAGATTAAGATAAAGTAATCAAACTCAAAGATGATGAAGGTGAGTTAATAATGTGTGGTAATTAAAATTGAACAATGCAGGCTGACGACAATAAAGATGGGTACTTAACAATCGATGAAATGCTTAATCATGAGTATGTTTTCTACAGCACAGTATATGAAAATCAGAATGGAGATTACGAAGATGATTACCATGATGAACTTTGATTTTTCTTCTTTTTTCCCCATTTTTTGAAGCTTAGATTTTAGATTTCATTTGCATATAAAGTGTATTGGGTTACAATTTTACTAAACCCTCCCACCCCTTGTGGATTTTGATTCTTTTTCGACCAGGA

mRNA sequence

ATGGGGAAGGCGGTGGTATATGTTCTTACAGCCACCGTCTTCGCTCTCTTCTTCCTAATTTCTCCCTCCAATTTCCACAATCGGAGCCATCAACAGGCAACTCGTCGTCTAGGATTCAAATTCCCAAACCCTACTTTCGATCCTCTTGTTACGGAAATGGAGAGATTAGCAGCAGAGGAACGTGGTGAAAATGCGATTGGAGTGGACAATCAAAACCATAAGATCATCGATTCTTATAGAAATTACTACGATGAAGGGAGATTGAACATTTCGTTGAGGTTACTTGTGTTGTTTCCTTTGCTGGATAATTCGCCTAAAGATGGGGTTATCAGTTACGAGGAGTTGAGTGATTGGATTAATGGGCAAGCTATTGAAAGATTGAATTATAGAACGACGAAACAATTGGAGTTCTACGATAAGAATGGAGATGACGCCATTTCATTCCATGAATATCTGCCGCAGTTCACTGAGGAAGATATTGCAAGAAATGAAACGGGATATGGAGAAGCTGGATGGTGGAGAAAGCAATTCACAAATGCGGATGTTGACAACAATGGCTTACTATATTTTGATGAACTTAAAGATTTCCTTCACCCAGAAGATAGCAGCAATTACCGAATACAAAACTGGCTGTTGGCACAGAAAATGAAGCGCATGGACCATGACAAAGATGGAAAGCTCAATTTTGATGAATTCCTTCACCATACATATGATATATACAAGAACTATATTGAATTTGAAACTCAAGGCGAAGATGTACCAAGCGCTGAAGAGAAGTTTGATGAGCTTGATCTCGACGAGGATGAAGTATTGTCAACGGAAGAATTAAGACCATTGTTCCAGTATCTACATCCTGGAGAAGTGTCTTATGCTCAACATTACACAAGTCATTTGATTAATGAGGCTGACGACAATAAAGATGGGTACTTAACAATCGATGAAATGCTTAATCATGAGTATGTTTTCTACAGCACAGTATATGAAAATCAGAATGGAGATTACGAAGATGATTACCATGATGAACTTTGA

Coding sequence (CDS)

ATGGGGAAGGCGGTGGTATATGTTCTTACAGCCACCGTCTTCGCTCTCTTCTTCCTAATTTCTCCCTCCAATTTCCACAATCGGAGCCATCAACAGGCAACTCGTCGTCTAGGATTCAAATTCCCAAACCCTACTTTCGATCCTCTTGTTACGGAAATGGAGAGATTAGCAGCAGAGGAACGTGGTGAAAATGCGATTGGAGTGGACAATCAAAACCATAAGATCATCGATTCTTATAGAAATTACTACGATGAAGGGAGATTGAACATTTCGTTGAGGTTACTTGTGTTGTTTCCTTTGCTGGATAATTCGCCTAAAGATGGGGTTATCAGTTACGAGGAGTTGAGTGATTGGATTAATGGGCAAGCTATTGAAAGATTGAATTATAGAACGACGAAACAATTGGAGTTCTACGATAAGAATGGAGATGACGCCATTTCATTCCATGAATATCTGCCGCAGTTCACTGAGGAAGATATTGCAAGAAATGAAACGGGATATGGAGAAGCTGGATGGTGGAGAAAGCAATTCACAAATGCGGATGTTGACAACAATGGCTTACTATATTTTGATGAACTTAAAGATTTCCTTCACCCAGAAGATAGCAGCAATTACCGAATACAAAACTGGCTGTTGGCACAGAAAATGAAGCGCATGGACCATGACAAAGATGGAAAGCTCAATTTTGATGAATTCCTTCACCATACATATGATATATACAAGAACTATATTGAATTTGAAACTCAAGGCGAAGATGTACCAAGCGCTGAAGAGAAGTTTGATGAGCTTGATCTCGACGAGGATGAAGTATTGTCAACGGAAGAATTAAGACCATTGTTCCAGTATCTACATCCTGGAGAAGTGTCTTATGCTCAACATTACACAAGTCATTTGATTAATGAGGCTGACGACAATAAAGATGGGTACTTAACAATCGATGAAATGCTTAATCATGAGTATGTTTTCTACAGCACAGTATATGAAAATCAGAATGGAGATTACGAAGATGATTACCATGATGAACTTTGA

Homology

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BLAST of CSPI03G19650 vs. Swiss-Prot
Match: RCN2V_CROAD (Reticulocalbin-2 OS=Crotalus adamanteus PE=1 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 2.2e-12
Identity = 74/264 (28.03%), Postives = 125/264 (47.35%), Query Frame = 1


Query: 93  RLLVLFPLLDNSPKDGVISYEELSDWINGQAIERLNYRTTKQLEFYDKNGDDAISFHEYL 152
           RL V+   +D    DG ++  ELS WI       +     +Q + YDK+GD  +S+ EY 
Sbjct: 54  RLKVIISRIDVD-LDGFLTEAELSSWIQHSFKSYIIEDAKQQFQHYDKDGDGRVSWEEYN 113

Query: 153 PQF----------TEEDIARNETGYGEAGWWRKQFTNADVDNNGLLYFDELKDFLHPEDS 212
            Q           T  D A  E+        +K+F  A+ D +  L F+E   F HPE++
Sbjct: 114 IQMYDRVIDFEEDTTLDDAEEESFRQLHLKDKKRFQKANKDGDSHLDFEEFAAFEHPEEA 173

Query: 213 SNYRIQNWLLAQKMKRMDHDKDGKLNFDEFLHHTYDIYKNYIEFETQGEDVPSAEEKF-D 272
               ++ +++ + ++  D D DG ++  EFL      Y+     +   E +   E++F +
Sbjct: 174 D--YMKEFVIQESLEEHDKDGDGFVSLQEFLGD----YRRDPAAKEDPEWIVVEEDRFKN 233

Query: 273 ELDLDEDEVLSTEELRPLFQYLHPGEVSYAQHYTSHLINEADDNKDGYLTIDEMLNHEYV 332
           + D D+D  LS +E   L  ++ P     AQ    HL++E D + D  L+ +E+L ++ +
Sbjct: 234 DYDKDKDGKLSPKE---LLTWVMPNNEGLAQEEAVHLLDEMDLDGDRRLSANEILENQDL 293

Query: 333 FY---STVYENQNGDYEDDYHDEL 343
           F    +T Y  Q  D +  YH+EL
Sbjct: 294 FLNSEATDYGRQLHD-KSFYHEEL 306

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BLAST of CSPI03G19650 vs. Swiss-Prot
Match: CALUA_DANRE (Calumenin-A OS=Danio rerio GN=calua PE=2 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 4.9e-12
Identity = 59/226 (26.11%), Postives = 112/226 (49.56%), Query Frame = 1


Query: 103 NSPKDGVISYEELSDWINGQAIERLNYRTTKQLEFYDKNGDDAISFHEY--LPQFTEEDI 162
           ++ +DG ++  EL  WI     + +     +Q + +D N D  IS+ EY  +   T  D 
Sbjct: 81  DADEDGFVTEAELKAWIKKAQKKYIYDNVERQWKDFDLNNDRMISWEEYKNVTYGTYLDD 140

Query: 163 ARNETGYGEAGWW---RKQFTNADVDNNGLLYFDELKDFLHPEDSSNYRIQNWLLAQKMK 222
              + GY          ++F  AD + + +   +E   FLHPE+  +  +++ ++ + M+
Sbjct: 141 PEPDDGYNYKQMMARDERRFKMADGNGDHIADKEEFTAFLHPEEYEH--MKDIVVLETME 200

Query: 223 RMDHDKDGKLNFDEFLHHTYDIYKNYIEFETQGEDVPSAEEKFDEL-DLDEDEVLSTEEL 282
            +D + DG ++ +E++   Y    N+ +   + E V +  E+F E  D ++D  +  EE 
Sbjct: 201 DIDKNGDGFIDLEEYIGDMY----NHEDEMDEPEWVATEREQFSEFRDKNKDGKMDREET 260

Query: 283 RPLFQYLHPGEVSYAQHYTSHLINEADDNKDGYLTIDEMLNHEYVF 323
                ++ P +  +A+    HL+ E+D NKDG LT +E+LN   +F
Sbjct: 261 ---MDWILPADYDHAEAEAKHLVYESDTNKDGKLTKEEILNKYDLF 297

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BLAST of CSPI03G19650 vs. Swiss-Prot
Match: CALU_RABIT (Calumenin OS=Oryctolagus cuniculus GN=CALU PE=1 SV=2)

HSP 1 Score: 66.2 bits (160), Expect = 7.8e-10
Identity = 62/236 (26.27%), Postives = 112/236 (47.46%), Query Frame = 1


Query: 103 NSPKDGVISYEELSDWINGQAIERLNYRTTKQLEFYDKNGDDAISFHEYLPQFTEEDIAR 162
           ++ KDG ++ +EL DWI       +     +Q + +D N D  +S+ EY          +
Sbjct: 81  DADKDGFVTVDELKDWIKFAQKRWIYEDVERQWKGHDLNEDGLVSWEEY----------K 140

Query: 163 NET-GY------GEAGWWRKQ--------FTNADVDNNGLLYFDELKDFLHPEDSSNYRI 222
           N T GY       + G+  KQ        F  AD D + +   +E   FLHPE+     +
Sbjct: 141 NATYGYVLDDPDPDDGFNYKQMMVRDERRFKMADKDGDLIATKEEFTAFLHPEEYD--YM 200

Query: 223 QNWLLAQKMKRMDHDKDGKLNFDEFLHHTYDIYKNYIEFETQGEDVPSAEEKFDEL-DLD 282
           ++ ++ + M+ +D + DG ++ +E++   Y    N      + E V +  E+F E  D +
Sbjct: 201 KDIVVQETMEDIDKNGDGFIDLEEYIGDMYSHDGN----ADEPEWVKTEREQFVEFRDKN 260

Query: 283 EDEVLSTEELRPLFQYLHPGEVSYAQHYTSHLINEADDNKDGYLTIDEMLNHEYVF 323
            D  +  EE +    ++ P +  +A+    HL+ E+D NKDG LT +E+++   +F
Sbjct: 261 RDGKMDKEETK---DWILPSDYDHAEAEARHLVYESDQNKDGKLTKEEIVDKYDLF 297

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BLAST of CSPI03G19650 vs. Swiss-Prot
Match: RCN1_TAKRU (Reticulocalbin-1 OS=Takifugu rubripes GN=rcn1 PE=3 SV=1)

HSP 1 Score: 59.7 bits (143), Expect = 7.3e-08
Identity = 61/238 (25.63%), Postives = 113/238 (47.48%), Query Frame = 1


Query: 106 KDGVISYEELSDWINGQAIERLNYRTTKQLEFYDKNGDDAISFHEYLPQFTEEDIARNET 165
           KD  IS+EE      G       Y  +   EF +    D  SF + LP        R+E 
Sbjct: 122 KDNKISWEEYKQATYG-------YYLSNPEEFDETT--DQFSFKKMLP--------RDE- 181

Query: 166 GYGEAGWWRKQFTNADVDNNGLLYFDELKDFLHPEDSSNYRIQNWLLAQKMKRMDHDKDG 225
                    ++F  AD+D +     +E   FLHPE+  +  +++ ++ + ++ +D + DG
Sbjct: 182 ---------RRFKRADLDGDSAANREEFTSFLHPEEFEH--MKDIVVLETLEDIDKNSDG 241

Query: 226 KLNFDEFLHHTYDIYKNYIEFETQGEDVPSAEEKFDEL-DLDEDEVLSTEELRPLFQYLH 285
            ++ DE++   +     + +   + E V +  E+F +  DL++D  +  +E+R    ++ 
Sbjct: 242 HVDEDEYIADMFA----HEDRGPEPEWVKTEREQFSDFRDLNKDGKMDLDEIR---HWIM 301

Query: 286 PGEVSYAQHYTSHLINEADDNKDGYLTIDEMLNHEYVFYSTVYENQNGDYEDDYHDEL 343
           P +  +AQ    HL+ E+D +KD  LT +E+L++  +F  +   N   D   + HDEL
Sbjct: 302 PQDYDHAQAEARHLVYESDKDKDQMLTKEEILDNWNMFVGSQATNYGEDLTRN-HDEL 322

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BLAST of CSPI03G19650 vs. Swiss-Prot
Match: RCN1_HUMAN (Reticulocalbin-1 OS=Homo sapiens GN=RCN1 PE=1 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 9.5e-08
Identity = 65/238 (27.31%), Postives = 113/238 (47.48%), Query Frame = 1


Query: 106 KDGVISYEELSDWINGQAIERLNYRTTKQLEFYDKNGDDAISFHEYLPQFTEEDIARNET 165
           KD  IS+EE      G       Y      EF+D +  D  +F + LP        R+E 
Sbjct: 131 KDDKISWEEYKQATYG-------YYLGNPAEFHDSS--DHHTFKKMLP--------RDE- 190

Query: 166 GYGEAGWWRKQFTNADVDNNGLLYFDELKDFLHPEDSSNYRIQNWLLAQKMKRMDHDKDG 225
                    ++F  AD++ +     +E   FLHPE+  +  ++  ++ + ++ +D + DG
Sbjct: 191 ---------RRFKAADLNGDLTATREEFTAFLHPEEFEH--MKEIVVLETLEDIDKNGDG 250

Query: 226 KLNFDEFLHHTYDIYKNYIEFETQGEDVPSAEEKFDEL-DLDEDEVLSTEELRPLFQYLH 285
            ++ DE++   +   +N  E +     V S  E+F+E  DL++D  L  +E+R    ++ 
Sbjct: 251 FVDQDEYIADMFSHEENGPEPDW----VLSEREQFNEFRDLNKDGKLDKDEIR---HWIL 310

Query: 286 PGEVSYAQHYTSHLINEADDNKDGYLTIDEMLNHEYVFYSTVYENQNGDYEDDYHDEL 343
           P +  +AQ    HL+ E+D NKD  LT +E+L +  +F  +   N   D   + HDEL
Sbjct: 311 PQDYDHAQAEARHLVYESDKNKDEKLTKEEILENWNMFVGSQATNYGEDLTKN-HDEL 331

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BLAST of CSPI03G19650 vs. TrEMBL
Match: A0A0A0LAH3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G238180 PE=4 SV=1)

HSP 1 Score: 706.4 bits (1822), Expect = 1.6e-200
Identity = 342/342 (100.00%), Postives = 342/342 (100.00%), Query Frame = 1


Query: 1   MGKAVVYVLTATVFALFFLISPSNFHNRSHQQATRRLGFKFPNPTFDPLVTEMERLAAEE 60
           MGKAVVYVLTATVFALFFLISPSNFHNRSHQQATRRLGFKFPNPTFDPLVTEMERLAAEE
Sbjct: 1   MGKAVVYVLTATVFALFFLISPSNFHNRSHQQATRRLGFKFPNPTFDPLVTEMERLAAEE 60

Query: 61  RGENAIGVDNQNHKIIDSYRNYYDEGRLNISLRLLVLFPLLDNSPKDGVISYEELSDWIN 120
           RGENAIGVDNQNHKIIDSYRNYYDEGRLNISLRLLVLFPLLDNSPKDGVISYEELSDWIN
Sbjct: 61  RGENAIGVDNQNHKIIDSYRNYYDEGRLNISLRLLVLFPLLDNSPKDGVISYEELSDWIN 120

Query: 121 GQAIERLNYRTTKQLEFYDKNGDDAISFHEYLPQFTEEDIARNETGYGEAGWWRKQFTNA 180
           GQAIERLNYRTTKQLEFYDKNGDDAISFHEYLPQFTEEDIARNETGYGEAGWWRKQFTNA
Sbjct: 121 GQAIERLNYRTTKQLEFYDKNGDDAISFHEYLPQFTEEDIARNETGYGEAGWWRKQFTNA 180

Query: 181 DVDNNGLLYFDELKDFLHPEDSSNYRIQNWLLAQKMKRMDHDKDGKLNFDEFLHHTYDIY 240
           DVDNNGLLYFDELKDFLHPEDSSNYRIQNWLLAQKMKRMDHDKDGKLNFDEFLHHTYDIY
Sbjct: 181 DVDNNGLLYFDELKDFLHPEDSSNYRIQNWLLAQKMKRMDHDKDGKLNFDEFLHHTYDIY 240

Query: 241 KNYIEFETQGEDVPSAEEKFDELDLDEDEVLSTEELRPLFQYLHPGEVSYAQHYTSHLIN 300
           KNYIEFETQGEDVPSAEEKFDELDLDEDEVLSTEELRPLFQYLHPGEVSYAQHYTSHLIN
Sbjct: 241 KNYIEFETQGEDVPSAEEKFDELDLDEDEVLSTEELRPLFQYLHPGEVSYAQHYTSHLIN 300

Query: 301 EADDNKDGYLTIDEMLNHEYVFYSTVYENQNGDYEDDYHDEL 343
           EADDNKDGYLTIDEMLNHEYVFYSTVYENQNGDYEDDYHDEL
Sbjct: 301 EADDNKDGYLTIDEMLNHEYVFYSTVYENQNGDYEDDYHDEL 342

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BLAST of CSPI03G19650 vs. TrEMBL
Match: M5X8P6_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa019586mg PE=4 SV=1)

HSP 1 Score: 387.5 bits (994), Expect = 1.7e-104
Identity = 198/357 (55.46%), Postives = 246/357 (68.91%), Query Frame = 1


Query: 1   MGKAVVYVLTATVFALFFLISPSNFH-NRSHQQATRRLGFKFPNPTFDPLVTEMERLAAE 60
           M KAVVY L AT F L  +  PS  H +  H    RRLGF+   P FDPLV +MER A E
Sbjct: 1   MAKAVVYALLATAFILVMVFCPSKQHGHHDHLGLNRRLGFRDHVPVFDPLVAKMERYAEE 60

Query: 61  ERGENAIGVDNQNHKI-------------IDSYRNYY-DEGRLNISLRLLVLFPLLDNSP 120
                  G+  Q H +             ++    Y+ DEG+LN++LRLL LFPL+D +P
Sbjct: 61  N------GLGGQTHNLDLVEHSPMMDTSEVEEVHEYFSDEGKLNLTLRLLSLFPLIDQAP 120

Query: 121 KDGVISYEELSDWINGQAIERLNYRTTKQLEFYDKNGDDAISFHEYLPQFTEEDIARNET 180
           KD  IS  EL  W+  QA+ERLNYRT K++E YDK+GD  ISF EY P+F+ +DI +N  
Sbjct: 121 KDSYISSNELKHWLTQQAVERLNYRTQKEMESYDKDGDGNISFSEYQPKFSSQDIKKNGM 180

Query: 181 GYGEAGWWRKQFTNADVDNNGLLYFDELKDFLHPEDSSNYRIQNWLLAQKMKRMDHDKDG 240
            +GEAGWW++QF NAD D NG L F+E KD LHPEDS+N  I  WLLA+KMKRMD D DG
Sbjct: 181 EHGEAGWWKQQFDNADADKNGTLTFNEFKDLLHPEDSNNSDIHKWLLAEKMKRMDQDDDG 240

Query: 241 KLNFDEFLHHTYDIYKNYIEFETQGEDVPSAEEKFDELDLDEDEVLSTEELRPLFQYLHP 300
           KLNF EF H+ YD +K+Y+EFET G   P+AEEKF ELDL++D +L  EEL+PL  YLHP
Sbjct: 241 KLNFMEFSHNAYDSFKSYVEFETGGVKAPTAEEKFAELDLNKDNLLEVEELKPLLHYLHP 300

Query: 301 GEVSYAQHYTSHLINEADDNKDGYLTIDEMLNHEYVFYSTVYE-NQNGDYEDDYHDE 342
           GE+SY+ +Y S L++EADDNKDG LT+DEML+H+YVFYSTVY+   N D E+DYHDE
Sbjct: 301 GELSYSNYYASFLMSEADDNKDGKLTLDEMLDHDYVFYSTVYDAGVNDDNEEDYHDE 351

[Close]

BLAST of CSPI03G19650 vs. TrEMBL
Match: B9IDN2_POPTR (Calcium-binding EF hand family protein OS=Populus trichocarpa GN=POPTR_0015s01260g PE=4 SV=1)

HSP 1 Score: 380.6 bits (976), Expect = 2.1e-102
Identity = 198/348 (56.90%), Postives = 250/348 (71.84%), Query Frame = 1


Query: 1   MGKAVVYVLTATVFALFFLISPSNFHNRSHQQATRRLGFKFPNPTFDPLVTEMERLAAEE 60
           MG+ VVYVL    F +F   SP N H R+     RRLG KF  P FDPLV +M+RLA E+
Sbjct: 1   MGRVVVYVLITIAFIVFMTFSPINNHGRTTPGLNRRLGSKFSMPDFDPLVVKMQRLAEEK 60

Query: 61  --RGEN-AIGVDNQNH--KIIDSYRNYYDEGRLNISLRLLVLFPLLDNSPKDGVISYEEL 120
              GE  AI ++N  +  ++ D+     D+GRLNI+LRLLVLFPLLD  P+DG+IS+EEL
Sbjct: 61  GYTGEGGAINLENNGYAKEVEDADEYLSDDGRLNITLRLLVLFPLLDKKPRDGLISFEEL 120

Query: 121 SDWINGQAIERLNYRTTKQLEFYDKNGDDAISFHEYLPQFTEEDIARNETGYGEAGWWRK 180
             W   QA ERL YRT ++++  DK+GD AI+F EYLPQF+ EDI RNE G+GEAGWW +
Sbjct: 121 EAWNVEQARERLAYRTQREIQSRDKDGDGAINFKEYLPQFSNEDIERNEMGHGEAGWWMQ 180

Query: 181 QFTNADVDNNGLLYFDELKDFLHPEDSSNYRIQNWLLAQKMKRMDHDKDGKLNFDEFLHH 240
           QF NADVD NG L FDE  +FLHPEDS+N  IQ W+L +K+KRMD D+DGKLN  EF  +
Sbjct: 181 QFRNADVDRNGTLDFDEFNNFLHPEDSNNKDIQKWILREKLKRMDDDRDGKLNLAEFSMY 240

Query: 241 TYD-IYKNYIEFETQGEDVPSAEEKFDELDLDEDEVLSTEELRPLFQYLHPGEVSYAQHY 300
            YD  YK+Y EFE     V +AEEKF ELD+++D  LS EEL P+  YL PGE+SYA+HY
Sbjct: 241 AYDGNYKSYAEFEPNVARVGTAEEKFLELDVNKDNFLSEEELIPMIPYLKPGELSYAKHY 300

Query: 301 TSHLINEADDNKDGYLTIDEMLNHEYVFYSTVYENQNGDYEDDYHDEL 343
           TS+LI+E D+N DGYL+IDEMLNHEY FY T +++   DY+D++H+EL
Sbjct: 301 TSYLIHETDENGDGYLSIDEMLNHEYTFYGTFFQDDE-DYDDEFHEEL 347

[Close]

BLAST of CSPI03G19650 vs. TrEMBL
Match: V4TRN0_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10023659mg PE=4 SV=1)

HSP 1 Score: 378.3 bits (970), Expect = 1.0e-101
Identity = 191/355 (53.80%), Postives = 252/355 (70.99%), Query Frame = 1


Query: 1   MGKAVVYVLTATVFALFFLISPSNFHNRSHQQAT--RRLGFKFPNPTFDPLVTEMERLAA 60
           M KAV Y L  T F LFF+ SP   H+ + + A   RR G+++  PTFDPLV +M R+  
Sbjct: 1   MSKAVAYALLTTAFVLFFVFSPIQPHDHNDRLAFIGRRFGYRYSVPTFDPLVLKMNRVTE 60

Query: 61  EERG------ENAIGVDNQ---NHKIIDSYRNYYDEGRLNISLRLLVLFPLLDNSPKDGV 120
           E++        NA  ++N    N    D Y  + + GRLNI+ RL+ LFPL+DN PKDGV
Sbjct: 61  EKKSGSIKTRTNANDLENDSYDNEDFEDEYDFFSENGRLNITSRLIYLFPLIDNEPKDGV 120

Query: 121 ISYEELSDWINGQAIERLNYRTTKQLEFYDKNGDDAISFHEYLPQFTEEDIARNETGYGE 180
           +S  EL +W    A++RL+Y T KQ+E  D++GD  ISF+EYLPQF+++DI +N   +G+
Sbjct: 121 LSLNELDNWNVELAVDRLSYTTQKQIELSDRDGDGEISFYEYLPQFSKQDIEKNGMVHGQ 180

Query: 181 AGWWRKQFTNADVDNNGLLYFDELKDFLHPEDSSNYRIQNWLLAQKMKRMDHDKDGKLNF 240
           AGWW++QF NADVD+NG L FDE  +FLHPEDS+N  IQ WLL +K++RMD D DGKLN 
Sbjct: 181 AGWWKEQFDNADVDSNGALNFDEFYNFLHPEDSNNTAIQRWLLREKLERMDDDNDGKLNL 240

Query: 241 DEFLHHTYDIYKNYIEFETQGE--DVPSAEEKFDELDLDEDEVLSTEELRPLFQYLHPGE 300
           DEFL +TY  YK+Y EFE  G+  D PSAEE F ELD ++D++L  EEL+P+F YLHPGE
Sbjct: 241 DEFLENTYRTYKSYAEFEDDGDGTDFPSAEETFVELDTNKDKLLEIEELKPIFSYLHPGE 300

Query: 301 VSYAQHYTSHLINEADDNKDGYLTIDEMLNHEYVFYSTVYENQNGDYEDDYHDEL 343
           +SYA++Y+ HLI+EADDN+DG LT+DEMLNHEY+FY+TVY N   D + D+ DEL
Sbjct: 301 ISYAKYYSRHLIHEADDNRDGNLTLDEMLNHEYIFYNTVY-NDVDDDDYDFRDEL 354

[Close]

BLAST of CSPI03G19650 vs. TrEMBL
Match: A0A058ZZR7_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_K00398 PE=4 SV=1)

HSP 1 Score: 378.3 bits (970), Expect = 1.0e-101
Identity = 192/346 (55.49%), Postives = 247/346 (71.39%), Query Frame = 1


Query: 1   MGKAVVYVLTATVFALFFLISPSNFHNRSHQQATRRLGFKFPNPTFDPLVTEMERLAAEE 60
           M KAVVY L AT    F + SP     R+H ++ RRLG    +PTFDPLVTE+ER     
Sbjct: 1   MAKAVVYALAATALMAFIIFSPMGQKGRNHLRSNRRLGIILSHPTFDPLVTELER---RR 60

Query: 61  RGENAIGVDNQNHKIIDS--YRNYY--DEGRLNISLRLLVLFPLLDNSPKDGVISYEELS 120
            GE+A   D      I S   RN Y  DEG LN + RLL LFPLLD++PKDG +S++EL 
Sbjct: 61  EGEHADREDPPASTEIGSPISRNEYLTDEGELNTTSRLLFLFPLLDDAPKDGAVSFKELE 120

Query: 121 DWINGQAIERLNYRTTKQLEFYDKNGDDAISFHEYLPQFTEEDIARNETGYGEAGWWRKQ 180
            W  GQA ERL Y+T K+L F+DK+GD AISF EYLPQF++EDI RN + +GEAGWW+++
Sbjct: 121 AWNVGQAHERLAYQTQKELAFFDKDGDGAISFREYLPQFSDEDIERNGSAHGEAGWWKEK 180

Query: 181 FTNADVDNNGLLYFDELKDFLHPEDSSNYRIQNWLLAQKMKRMDHDKDGKLNFDEFLHHT 240
           F NADVD NG L F+E  DFLHPEDS+N  I  WLL QK+K+MD+D DGKLN DEF  H 
Sbjct: 181 FLNADVDQNGTLSFNEFMDFLHPEDSNNTEIWKWLLWQKLKQMDYDGDGKLNLDEFKDHI 240

Query: 241 YDIYKNYIEFETQGEDVPSAEEKFDELDLDEDEVLSTEELRPLFQYLHPGEVSYAQHYTS 300
           YDIYKNY+EF+  G  VP+ E+KF  LD++ +++L  EE+ P+  YL+PGE+S+A++YT+
Sbjct: 241 YDIYKNYVEFDAHGGHVPTPEQKFTNLDVNNNKLLEVEEMIPILHYLNPGELSHAKYYTA 300

Query: 301 HLINEADDNKDGYLTIDEMLNHEYVFYSTVYENQNGDYEDDYHDEL 343
            LI EADDNKDG L+++EMLNHEY+FY++++E    D  D+ HDEL
Sbjct: 301 FLIREADDNKDGKLSLEEMLNHEYMFYASLFEEDEED--DEMHDEL 341

[Close]

BLAST of CSPI03G19650 vs. TAIR10
Match: AT4G27790.1 (AT4G27790.1 Calcium-binding EF hand family protein)

HSP 1 Score: 297.4 bits (760), Expect = 1.2e-80
Identity = 157/346 (45.38%), Postives = 224/346 (64.74%), Query Frame = 1


Query: 1   MGKAVVY-VLTATVFALFFLISPSNFHNRSHQQA-TRRLGFKFPNPTFDPLVTEMERLAA 60
           M K VVY +LTAT+  L  L        +S +   TRR+G +   P FDPLVT +ERL+ 
Sbjct: 1   MAKVVVYTILTATIIFLILLAHKKQNQTQSIEGLITRRIGRRLEMPVFDPLVTRIERLSH 60

Query: 61  EERGENAIGVDNQNHKIIDSYRNYY-DEGRLNISLRLLVLFPLLDNSPKDGVISYEELSD 120
           E+       V+    +  D +  Y+  E RLN ++R+  LFPLLD SP+DG +S +EL  
Sbjct: 61  EKEAGTKT-VEAAKEEKDDMFEEYFAQERRLNTTMRIKFLFPLLDASPRDGFVSLKELQT 120

Query: 121 WINGQAIERLNYRTTKQLEFYDKNGDDAISFHEYLPQFTEEDIARNETGYGEAGWWRKQF 180
           W+  Q  + + YRT K+LE  DK+ D  I+F EYLPQF+++DI +NE G+GEAGWW +QF
Sbjct: 121 WMMQQTEDNMVYRTAKELELQDKDKDGVITFEEYLPQFSKQDIEKNEKGHGEAGWWMEQF 180

Query: 181 TNADVDNNGLLYFDELKDFLHPEDSSNYRIQNWLLAQKMKRMDHDKDGKLNFDEFLHHTY 240
            N+D D+NG L  +E  +FLHPEDS N   Q W+L ++M  MD + DGKL + EF+ + Y
Sbjct: 181 KNSDFDHNGSLDIEEFNNFLHPEDSRNGDTQRWVLKERMTGMDTNGDGKLEYKEFVKNAY 240

Query: 241 DIYKNYIEFE-TQGEDVPSAEEKFDELDLDEDEVLSTEELRPLFQYLHPGEVSYAQHYTS 300
           ++YK + +FE  + E+VP+ +  F E+D D+D  L  +ELRP+ QYL PGE+SYA+ Y++
Sbjct: 241 EMYKEFAKFEKEEDENVPTPQLLFAEMDRDKDRFLVADELRPILQYLQPGEMSYAKFYST 300

Query: 301 HLINEADDNKDGYLTIDEMLNHEYVFYSTVYENQNGDYEDDYHDEL 343
            L +EAD++KDG L+++EML+HE VFY  V+     D +   HDEL
Sbjct: 301 FLCHEADEDKDGKLSLEEMLHHEDVFYKAVHHEDLDDEDYFDHDEL 345

[Close]

BLAST of CSPI03G19650 vs. TAIR10
Match: AT5G08580.1 (AT5G08580.1 Calcium-binding EF hand family protein)

HSP 1 Score: 201.1 bits (510), Expect = 1.1e-51
Identity = 130/391 (33.25%), Postives = 202/391 (51.66%), Query Frame = 1


Query: 1   MGKAVVYVLTATVFALFFLISPS-------NFHNRSHQQATR---RLGFKFPNPT----- 60
           M KA V +       + FL+S S       + H+  H Q  R   R  F F  PT     
Sbjct: 1   MSKASVILYITVGILVLFLVSYSPKKKGDHDHHHGGHNQHHRLKLRSSFNF-KPTRHDPV 60

Query: 61  -FDPLVTEMERLAAEERGE-------------------------NAIGVDNQNH--KIID 120
            FDPLV +MER   ++  E                         +A G ++Q    + +D
Sbjct: 61  PFDPLVADMERRREDKEWERQYIEHSHPELVSHSQKETTGGGHEHAPGHESQPEWEEFMD 120

Query: 121 SYRNYYDEGRLNISLRLLVLFPLLDNSPKDGVISYEELSDWINGQAIERLNYRTTKQLEF 180
           +     DE + N++ RL++LFP +D SP DG ++  EL++W    + + + +RT + L+ 
Sbjct: 121 AEDYLNDEEKFNVTDRLILLFPKIDVSPADGFMTESELTEWTMQSSAKEVVHRTQRDLDV 180

Query: 181 YDKNGDDAISFHEYLPQFTEEDIARNETGYGEAGWWRKQFTNA-DVDNNGLLYFDELKDF 240
           +D+N D  ISF EY P         N  GY + GWW+++  NA D + +GLL   E  DF
Sbjct: 181 HDRNKDGFISFSEYEPPSWVRKSDNNSFGY-DMGWWKEEHFNASDANGDGLLNLTEFNDF 240

Query: 241 LHPEDSSNYRIQNWLLAQKMKRMDHDKDGKLNFDEFLHHTYDIYKNYIEFETQG----ED 300
           LHP D+ N ++  WL  ++++  D DKDGK++F+EF H  +D  +NY E          D
Sbjct: 241 LHPADTKNPKLLLWLCKEEVRERDSDKDGKISFEEFFHGLFDTVRNYEEDNHNSTHPYHD 300

Query: 301 VPS--AEEKFDELDLDEDEVLSTEELRPLFQYLHPGEVSYAQHYTSHLINEADDNKDGYL 342
           +P   A++ F +LD ++D  LS  EL P+   +HP E  YA+    ++I++AD +KD  L
Sbjct: 301 LPEGPAKQLFSQLDKNDDGYLSDVELLPIISKIHPTEHYYAKQQADYIISQADSDKDRRL 360

[Close]

BLAST of CSPI03G19650 vs. TAIR10
Match: AT1G74740.1 (AT1G74740.1 calcium-dependent protein kinase 30)

HSP 1 Score: 50.8 bits (120), Expect = 1.9e-06
Identity = 37/137 (27.01%), Postives = 63/137 (45.99%), Query Frame = 1


Query: 97  LFPLLDNSPKDGVISYEELSDWINGQAIERLNYRTTKQLEFYDKNGDDAISFHEYLPQFT 156
           +F L+D+   DG ISY EL   +     +         +E  D NG+  + + E++    
Sbjct: 368 MFTLMDDD-NDGKISYLELRAGLRKVGSQLGEPEIKLLMEVADVNGNGCLDYGEFVAVII 427

Query: 157 EEDIARNETGYGEAGWWRKQFTNADVDNNGLLYFDELKDFLHPEDSSNYRIQNWLLAQKM 216
                 N+  +      R+ F   D D +G +  +EL++ L  E        N ++   M
Sbjct: 428 HLQKMENDEHF------RQAFMFFDKDGSGYIESEELREALTDELGEP---DNSVIIDIM 487

Query: 217 KRMDHDKDGKLNFDEFL 234
           + +D DKDGK+N+DEF+
Sbjct: 488 REVDTDKDGKINYDEFV 494

[Close]

BLAST of CSPI03G19650 vs. NCBI nr
Match: gi|778680426|ref|XP_011651314.1| (PREDICTED: reticulocalbin-2 [Cucumis sativus])

HSP 1 Score: 706.4 bits (1822), Expect = 2.4e-200
Identity = 342/342 (100.00%), Postives = 342/342 (100.00%), Query Frame = 1


Query: 1   MGKAVVYVLTATVFALFFLISPSNFHNRSHQQATRRLGFKFPNPTFDPLVTEMERLAAEE 60
           MGKAVVYVLTATVFALFFLISPSNFHNRSHQQATRRLGFKFPNPTFDPLVTEMERLAAEE
Sbjct: 1   MGKAVVYVLTATVFALFFLISPSNFHNRSHQQATRRLGFKFPNPTFDPLVTEMERLAAEE 60

Query: 61  RGENAIGVDNQNHKIIDSYRNYYDEGRLNISLRLLVLFPLLDNSPKDGVISYEELSDWIN 120
           RGENAIGVDNQNHKIIDSYRNYYDEGRLNISLRLLVLFPLLDNSPKDGVISYEELSDWIN
Sbjct: 61  RGENAIGVDNQNHKIIDSYRNYYDEGRLNISLRLLVLFPLLDNSPKDGVISYEELSDWIN 120

Query: 121 GQAIERLNYRTTKQLEFYDKNGDDAISFHEYLPQFTEEDIARNETGYGEAGWWRKQFTNA 180
           GQAIERLNYRTTKQLEFYDKNGDDAISFHEYLPQFTEEDIARNETGYGEAGWWRKQFTNA
Sbjct: 121 GQAIERLNYRTTKQLEFYDKNGDDAISFHEYLPQFTEEDIARNETGYGEAGWWRKQFTNA 180

Query: 181 DVDNNGLLYFDELKDFLHPEDSSNYRIQNWLLAQKMKRMDHDKDGKLNFDEFLHHTYDIY 240
           DVDNNGLLYFDELKDFLHPEDSSNYRIQNWLLAQKMKRMDHDKDGKLNFDEFLHHTYDIY
Sbjct: 181 DVDNNGLLYFDELKDFLHPEDSSNYRIQNWLLAQKMKRMDHDKDGKLNFDEFLHHTYDIY 240

Query: 241 KNYIEFETQGEDVPSAEEKFDELDLDEDEVLSTEELRPLFQYLHPGEVSYAQHYTSHLIN 300
           KNYIEFETQGEDVPSAEEKFDELDLDEDEVLSTEELRPLFQYLHPGEVSYAQHYTSHLIN
Sbjct: 241 KNYIEFETQGEDVPSAEEKFDELDLDEDEVLSTEELRPLFQYLHPGEVSYAQHYTSHLIN 300

Query: 301 EADDNKDGYLTIDEMLNHEYVFYSTVYENQNGDYEDDYHDEL 343
           EADDNKDGYLTIDEMLNHEYVFYSTVYENQNGDYEDDYHDEL
Sbjct: 301 EADDNKDGYLTIDEMLNHEYVFYSTVYENQNGDYEDDYHDEL 342

[Close]

BLAST of CSPI03G19650 vs. NCBI nr
Match: gi|659111942|ref|XP_008455987.1| (PREDICTED: reticulocalbin-2 [Cucumis melo])

HSP 1 Score: 665.2 bits (1715), Expect = 6.0e-188
Identity = 319/343 (93.00%), Postives = 334/343 (97.38%), Query Frame = 1


Query: 1   MGKAVVYVLTATVFALFFLISPSNFHNRSHQQATRRLGFKFPNPTFDPLVTEMERLAAEE 60
           MGKAVVYVLTAT FA+FFLISPSNFH+R++QQ TRRLGFKFPNPTFDPLVTEMERL AEE
Sbjct: 1   MGKAVVYVLTATAFAVFFLISPSNFHSRNYQQPTRRLGFKFPNPTFDPLVTEMERLEAEE 60

Query: 61  RGENAIGVDNQNHKIIDSYRNYYDEGRLNISLRLLVLFPLLDNSPKDGVISYEELSDWIN 120
           RGENAIGV+NQNHKI D+YRNYYD+GRLNISLRLLVLFPLLDNSPKDGVISYEELSDWIN
Sbjct: 61  RGENAIGVENQNHKITDAYRNYYDDGRLNISLRLLVLFPLLDNSPKDGVISYEELSDWIN 120

Query: 121 GQAIERLNYRTTKQLEFYDKNGDDAISFHEYLPQFTEEDIARNETGYGEAGWWRKQFTNA 180
           GQAIERLNYRTTKQLEFYDKNGD+AISFHEYLPQFT+EDIARNETGYGEAGWW KQFTNA
Sbjct: 121 GQAIERLNYRTTKQLEFYDKNGDNAISFHEYLPQFTKEDIARNETGYGEAGWWIKQFTNA 180

Query: 181 DVDNNGLLYFDELKDFLHPEDSSNYRIQNWLLAQKMKRMDHDKDGKLNFDEFLHHTYDIY 240
           DVD NGLL+FDELKDFLHPEDSSNYRIQNWLLAQKMKRMDHD+DGKLNFDEFLHHTYDIY
Sbjct: 181 DVDQNGLLHFDELKDFLHPEDSSNYRIQNWLLAQKMKRMDHDRDGKLNFDEFLHHTYDIY 240

Query: 241 KNYIEFETQGEDVPSAEEKFDELDLDEDEVLSTEELRPLFQYLHPGEVSYAQHYTSHLIN 300
           KNYIEFETQG+D+P+AEEKFDELDLDEDEVLS EE+RPLFQYLHPGEVSYAQHYTSHLIN
Sbjct: 241 KNYIEFETQGDDIPTAEEKFDELDLDEDEVLSVEEIRPLFQYLHPGEVSYAQHYTSHLIN 300

Query: 301 EADDNKDGYLTIDEMLNHEYVFYSTVYENQNGDYEDD-YHDEL 343
           EADDNKDGYLTIDEMLNHEY FYSTVYENQNGDYEDD YHDEL
Sbjct: 301 EADDNKDGYLTIDEMLNHEYAFYSTVYENQNGDYEDDYYHDEL 343

[Close]

BLAST of CSPI03G19650 vs. NCBI nr
Match: gi|1009138494|ref|XP_015886617.1| (PREDICTED: calumenin-B [Ziziphus jujuba])

HSP 1 Score: 397.5 bits (1020), Expect = 2.3e-107
Identity = 200/360 (55.56%), Postives = 260/360 (72.22%), Query Frame = 1


Query: 1   MGKAVVYVLTATVFALFFLISPSNFH-NRSHQQATRRLGFKFPNPTFDPLVTEMERLAAE 60
           M KAV Y L AT F +F + +P+N     +H    RRLG  F   TFDPLVT+++R A E
Sbjct: 1   MAKAVAYALLATAFVIFVVFAPANPPVPPNHPGINRRLGSSFGLSTFDPLVTKIQRYAEE 60

Query: 61  ERG------ENAIGVDNQN-----------HKIIDSYRNYYDEGRLNISLRLLVLFPLLD 120
            +G      +N +G D+ +           ++I D+ + + +EGRLNI+LRL++LFPLLD
Sbjct: 61  RKGYDHHQHDNIVGDDDDDDDDNNNNNGFANEIPDASQYFGEEGRLNITLRLIILFPLLD 120

Query: 121 NSPKDGVISYEELSDWINGQAIERLNYRTTKQLEFYDKNGDDAISFHEYLPQFTEEDIAR 180
            SPKDGV+S  EL  WI  QA+ERLNY T K+L  +DKNGD  ISF EYLPQF++EDI +
Sbjct: 121 KSPKDGVVSTWELRSWITEQAVERLNYTTQKELASHDKNGDGVISFGEYLPQFSDEDIEK 180

Query: 181 NETGYGEAGWWRKQFTNADVDNNGLLYFDELKDFLHPEDSSNYRIQNWLLAQKMKRMDHD 240
           N T +GEAGWW++QF NADVD +G L FDE KD LHP+DS N +IQNWLL +K+KRMD +
Sbjct: 181 NGTAHGEAGWWKQQFMNADVDGDGSLSFDEFKDLLHPQDSKNDKIQNWLLREKIKRMDQN 240

Query: 241 KDGKLNFDEFLHHTYDIYKNYIEFETQGEDVPSAEEKFDELDLDEDEVLSTEELRPLFQY 300
            DGKLNF EF    Y IYKNY+E+ET G DVP+AEEKF ELDL++D+ L  EEL+P+  Y
Sbjct: 241 DDGKLNFVEFSDSAYSIYKNYVEYETSGADVPTAEEKFAELDLNKDKFLEVEELKPMLTY 300

Query: 301 LHPGEVSYAQHYTSHLINEADDNKDGYLTIDEMLNHEYVFYSTVYENQNGDYEDDYHDEL 343
           LHPGE SYA++YT++L++EAD+NKDG L++ EMLNHE++FYSTVY +   D  DD+H+EL
Sbjct: 301 LHPGEFSYAKYYTNYLLHEADENKDGNLSLLEMLNHEHIFYSTVYSDSVEDDGDDFHEEL 360

[Close]

BLAST of CSPI03G19650 vs. NCBI nr
Match: gi|596062008|ref|XP_007221135.1| (hypothetical protein PRUPE_ppa019586mg [Prunus persica])

HSP 1 Score: 387.5 bits (994), Expect = 2.4e-104
Identity = 198/357 (55.46%), Postives = 246/357 (68.91%), Query Frame = 1


Query: 1   MGKAVVYVLTATVFALFFLISPSNFH-NRSHQQATRRLGFKFPNPTFDPLVTEMERLAAE 60
           M KAVVY L AT F L  +  PS  H +  H    RRLGF+   P FDPLV +MER A E
Sbjct: 1   MAKAVVYALLATAFILVMVFCPSKQHGHHDHLGLNRRLGFRDHVPVFDPLVAKMERYAEE 60

Query: 61  ERGENAIGVDNQNHKI-------------IDSYRNYY-DEGRLNISLRLLVLFPLLDNSP 120
                  G+  Q H +             ++    Y+ DEG+LN++LRLL LFPL+D +P
Sbjct: 61  N------GLGGQTHNLDLVEHSPMMDTSEVEEVHEYFSDEGKLNLTLRLLSLFPLIDQAP 120

Query: 121 KDGVISYEELSDWINGQAIERLNYRTTKQLEFYDKNGDDAISFHEYLPQFTEEDIARNET 180
           KD  IS  EL  W+  QA+ERLNYRT K++E YDK+GD  ISF EY P+F+ +DI +N  
Sbjct: 121 KDSYISSNELKHWLTQQAVERLNYRTQKEMESYDKDGDGNISFSEYQPKFSSQDIKKNGM 180

Query: 181 GYGEAGWWRKQFTNADVDNNGLLYFDELKDFLHPEDSSNYRIQNWLLAQKMKRMDHDKDG 240
            +GEAGWW++QF NAD D NG L F+E KD LHPEDS+N  I  WLLA+KMKRMD D DG
Sbjct: 181 EHGEAGWWKQQFDNADADKNGTLTFNEFKDLLHPEDSNNSDIHKWLLAEKMKRMDQDDDG 240

Query: 241 KLNFDEFLHHTYDIYKNYIEFETQGEDVPSAEEKFDELDLDEDEVLSTEELRPLFQYLHP 300
           KLNF EF H+ YD +K+Y+EFET G   P+AEEKF ELDL++D +L  EEL+PL  YLHP
Sbjct: 241 KLNFMEFSHNAYDSFKSYVEFETGGVKAPTAEEKFAELDLNKDNLLEVEELKPLLHYLHP 300

Query: 301 GEVSYAQHYTSHLINEADDNKDGYLTIDEMLNHEYVFYSTVYE-NQNGDYEDDYHDE 342
           GE+SY+ +Y S L++EADDNKDG LT+DEML+H+YVFYSTVY+   N D E+DYHDE
Sbjct: 301 GELSYSNYYASFLMSEADDNKDGKLTLDEMLDHDYVFYSTVYDAGVNDDNEEDYHDE 351

[Close]

BLAST of CSPI03G19650 vs. NCBI nr
Match: gi|224132718|ref|XP_002321392.1| (calcium-binding EF hand family protein [Populus trichocarpa])

HSP 1 Score: 380.6 bits (976), Expect = 3.0e-102
Identity = 198/348 (56.90%), Postives = 250/348 (71.84%), Query Frame = 1


Query: 1   MGKAVVYVLTATVFALFFLISPSNFHNRSHQQATRRLGFKFPNPTFDPLVTEMERLAAEE 60
           MG+ VVYVL    F +F   SP N H R+     RRLG KF  P FDPLV +M+RLA E+
Sbjct: 1   MGRVVVYVLITIAFIVFMTFSPINNHGRTTPGLNRRLGSKFSMPDFDPLVVKMQRLAEEK 60

Query: 61  --RGEN-AIGVDNQNH--KIIDSYRNYYDEGRLNISLRLLVLFPLLDNSPKDGVISYEEL 120
              GE  AI ++N  +  ++ D+     D+GRLNI+LRLLVLFPLLD  P+DG+IS+EEL
Sbjct: 61  GYTGEGGAINLENNGYAKEVEDADEYLSDDGRLNITLRLLVLFPLLDKKPRDGLISFEEL 120

Query: 121 SDWINGQAIERLNYRTTKQLEFYDKNGDDAISFHEYLPQFTEEDIARNETGYGEAGWWRK 180
             W   QA ERL YRT ++++  DK+GD AI+F EYLPQF+ EDI RNE G+GEAGWW +
Sbjct: 121 EAWNVEQARERLAYRTQREIQSRDKDGDGAINFKEYLPQFSNEDIERNEMGHGEAGWWMQ 180

Query: 181 QFTNADVDNNGLLYFDELKDFLHPEDSSNYRIQNWLLAQKMKRMDHDKDGKLNFDEFLHH 240
           QF NADVD NG L FDE  +FLHPEDS+N  IQ W+L +K+KRMD D+DGKLN  EF  +
Sbjct: 181 QFRNADVDRNGTLDFDEFNNFLHPEDSNNKDIQKWILREKLKRMDDDRDGKLNLAEFSMY 240

Query: 241 TYD-IYKNYIEFETQGEDVPSAEEKFDELDLDEDEVLSTEELRPLFQYLHPGEVSYAQHY 300
            YD  YK+Y EFE     V +AEEKF ELD+++D  LS EEL P+  YL PGE+SYA+HY
Sbjct: 241 AYDGNYKSYAEFEPNVARVGTAEEKFLELDVNKDNFLSEEELIPMIPYLKPGELSYAKHY 300

Query: 301 TSHLINEADDNKDGYLTIDEMLNHEYVFYSTVYENQNGDYEDDYHDEL 343
           TS+LI+E D+N DGYL+IDEMLNHEY FY T +++   DY+D++H+EL
Sbjct: 301 TSYLIHETDENGDGYLSIDEMLNHEYTFYGTFFQDDE-DYDDEFHEEL 347

The following BLAST results are available for this feature:

BLAST of CSPI03G19650 vs. Swiss-Prot


Match Name	E-value	Identity	Description
RCN2V_CROAD	2.2e-12	28.03	Reticulocalbin-2 OS=Crotalus adamanteus PE=1 SV=1	[more]
CALUA_DANRE	4.9e-12	26.11	Calumenin-A OS=Danio rerio GN=calua PE=2 SV=1	[more]
CALU_RABIT	7.8e-10	26.27	Calumenin OS=Oryctolagus cuniculus GN=CALU PE=1 SV=2	[more]
RCN1_TAKRU	7.3e-08	25.63	Reticulocalbin-1 OS=Takifugu rubripes GN=rcn1 PE=3 SV=1	[more]
RCN1_HUMAN	9.5e-08	27.31	Reticulocalbin-1 OS=Homo sapiens GN=RCN1 PE=1 SV=1	[more]

BLAST of CSPI03G19650 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LAH3_CUCSA	1.6e-200	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G238180 PE=4 SV=1	[more]
M5X8P6_PRUPE	1.7e-104	55.46	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa019586mg PE=4 SV=1	[more]
B9IDN2_POPTR	2.1e-102	56.90	Calcium-binding EF hand family protein OS=Populus trichocarpa GN=POPTR_0015s0126...	[more]
V4TRN0_9ROSI	1.0e-101	53.80	Uncharacterized protein OS=Citrus clementina GN=CICLE_v10023659mg PE=4 SV=1	[more]
A0A058ZZR7_EUCGR	1.0e-101	55.49	Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_K00398 PE=4 SV=1	[more]

BLAST of CSPI03G19650 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G27790.1	1.2e-80	45.38	Calcium-binding EF hand family protein	[more]
AT5G08580.1	1.1e-51	33.25	Calcium-binding EF hand family protein	[more]
AT1G74740.1	1.9e-06	27.01	calcium-dependent protein kinase 30	[more]

BLAST of CSPI03G19650 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778680426\|ref\|XP_011651314.1\|	2.4e-200	100.00	PREDICTED: reticulocalbin-2 [Cucumis sativus]	[more]
gi\|659111942\|ref\|XP_008455987.1\|	6.0e-188	93.00	PREDICTED: reticulocalbin-2 [Cucumis melo]	[more]
gi\|1009138494\|ref\|XP_015886617.1\|	2.3e-107	55.56	PREDICTED: calumenin-B [Ziziphus jujuba]	[more]
gi\|596062008\|ref\|XP_007221135.1\|	2.4e-104	55.46	hypothetical protein PRUPE_ppa019586mg [Prunus persica]	[more]
gi\|224132718\|ref\|XP_002321392.1\|	3.0e-102	56.90	calcium-binding EF hand family protein [Populus trichocarpa]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR002048	EF_hand_dom
IPR011992	EF-hand-dom_pair
IPR018247	EF_Hand_1_Ca_BS
IPR019577	SPARC/Testican_Ca-bd-dom

Vocabulary: **Molecular Function**
Term	Definition
GO:0005509	calcium ion binding

Vocabulary: **Cellular Component**
Term	Definition
GO:0005578	proteinaceous extracellular matrix

Vocabulary: **Biological Process**
Term	Definition
GO:0007165	signal transduction

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0007165	signal transduction
biological_process	GO:0008150	biological_process
cellular_component	GO:0005578	proteinaceous extracellular matrix
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0005509	calcium ion binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G19650.1	CSPI03G19650.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 172..200 score: 2.5coord: 211..239 score: 2.4coord: 294..322 score: 110.0coord: 130..158 score: 100.0coord: 255..283 score:
IPR002048	EF-hand domain	PROFILE	PS50222	EF_HAND_2	coord: 212..242 score: 8.655coord: 106..125 score: 7.177coord: 290..325 score: 5.838coord: 168..203 score: 8.153coord: 138..161 score: 6.284coord: 251..286 score: 9
IPR011992	EF-hand domain pair	GENE3D	G3DSA:1.10.238.10		coord: 185..318 score: 6.7E-21coord: 98..184 score: 3.
IPR011992	EF-hand domain pair	unknown	SSF47473	EF-hand	coord: 170..333 score: 5.22E-24coord: 93..153 score: 1.0
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 139..151 score: -coord: 303..315 score: -coord: 220..232 scor
IPR019577	SPARC/Testican, calcium-binding domain	PFAM	PF10591	SPARC_Ca_bdg	coord: 205..315 score: 1.
None	No IPR available	PANTHER	PTHR10827	RETICULOCALBIN	coord: 1..342 score: 2.2E
None	No IPR available	PANTHER	PTHR10827:SF54	CALCIUM-BINDING EF HAND FAMILY PROTEIN	coord: 1..342 score: 2.2E

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CSPI03G19650	Cla004270	Watermelon (97103) v1	cpiwmB242
CSPI03G19650	Csa3G238180	Cucumber (Chinese Long) v2	cpicuB120
CSPI03G19650	MELO3C019374	Melon (DHL92) v3.5.1	cpimeB241
CSPI03G19650	ClCG05G009070	Watermelon (Charleston Gray)	cpiwcgB237
CSPI03G19650	Lsi05G001150	Bottle gourd (USVL1VR-Ls)	cpilsiB209
CSPI03G19650	MELO3C019374.2	Melon (DHL92) v3.6.1	cpimedB239
CSPI03G19650	CsaV3_3G019710	Cucumber (Chinese Long) v3	cpicucB144
CSPI03G19650	Cla97C05G091390	Watermelon (97103) v2	cpiwmbB221
CSPI03G19650	Bhi01G001129	Wax gourd	cpiwgoB283
CSPI03G19650	Cucsa.032300	Cucumber (Gy14) v1	cgycpiB003
CSPI03G19650	CmaCh18G013200	Cucurbita maxima (Rimu)	cmacpiB414
CSPI03G19650	CmoCh18G013500	Cucurbita moschata (Rifu)	cmocpiB402
CSPI03G19650	Cp4.1LG09g00260	Cucurbita pepo (Zucchini)	cpecpiB035
CSPI03G19650	CsGy3G019580	Cucumber (Gy14) v2	cgybcpiB110
CSPI03G19650	Carg20338	Silver-seed gourd	carcpiB0575

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CSPI03G19650	Cucurbita maxima (Rimu)	cmacpiB290
CSPI03G19650	Cucurbita maxima (Rimu)	cmacpiB327
CSPI03G19650	Cucurbita maxima (Rimu)	cmacpiB417
CSPI03G19650	Cucurbita moschata (Rifu)	cmocpiB280
CSPI03G19650	Cucurbita moschata (Rifu)	cmocpiB318
CSPI03G19650	Cucurbita moschata (Rifu)	cmocpiB405
CSPI03G19650	Cucurbita moschata (Rifu)	cmocpiB601
CSPI03G19650	Cucumber (Chinese Long) v2	cpicuB139
CSPI03G19650	Melon (DHL92) v3.5.1	cpimeB169
CSPI03G19650	Watermelon (Charleston Gray)	cpiwcgB207
CSPI03G19650	Watermelon (97103) v1	cpiwmB258
CSPI03G19650	Cucurbita pepo (Zucchini)	cpecpiB032
CSPI03G19650	Cucurbita pepo (Zucchini)	cpecpiB209
CSPI03G19650	Cucurbita pepo (Zucchini)	cpecpiB723
CSPI03G19650	Bottle gourd (USVL1VR-Ls)	cpilsiB151
CSPI03G19650	Melon (DHL92) v3.6.1	cpimedB160
CSPI03G19650	Cucumber (Gy14) v2	cgybcpiB210
CSPI03G19650	Silver-seed gourd	carcpiB0573
CSPI03G19650	Silver-seed gourd	carcpiB1024
CSPI03G19650	Cucumber (Chinese Long) v3	cpicucB166
CSPI03G19650	Watermelon (97103) v2	cpiwmbB177
CSPI03G19650	Wax gourd	cpiwgoB263
CSPI03G19650	Wild cucumber (PI 183967)	cpicpiB114
CSPI03G19650	Cucumber (Gy14) v1	cgycpiB321