CSPI03G16010 (gene) Wild cucumber (PI 183967) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CSPI03G16010
Type	gene
Organism	Cucumis sativus (Wild cucumber (PI 183967))
Description	Plant invertase/pectin methylesterase inhibitor
Location	Chr3 : 11993053 .. 11994154 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CCCTTACACAACCCTTTTACACCTTTTTCATTTGCTTATAAATACAAAGCACATTCCGCTTCTTTCTCTTCATCACTTCCCCTTCTTTCTAAATTCCTTCCATTTACATTTAAGAAAACAAAGAAAACAGAGATGGGTATCTCAAACTCCAAGAAAACTTTGATTCTTTTCCAACTAATTCTCCAATTAACACTAATCCATTCAGTCATAACTCCACAAAGCAGCACAGAGTTCATAAGAAGCTCTTGCAGTTCCACCACTTACCCTCGTCTCTGTTTTTCATCTCTTTCAGTCCATGCCAATGCCATCCAAACAAGTCCCCGCCTTTTGGCCACTGCCGCCCTCTCCGTTTCCCTCTCCTCCGTCAAGTCCACCGCCACTCAAATCCTCAAGCTTTCTCACTCCCATGGCCTCCCCTCCAGAGATGTCTCTGCTCTTGACGACTGTTTGGAAGAACTGAGTGACTCTGTCGACTCCCTCGCTGCTTCCATTTCCGAGATGCCGAAGCTCCGTGGAACCAATTTCGACCTTGCAATGAGTAATGTTCAGACATGGGTTAGCGCCGCTCTGACGGATGAAACCACATGTAGCGAAGGGTTTCAGGGGAAGACGGTGAACGGAGGCGTTAAGGCTGAGGTTAGGACTAAGATTGTCAACATTGCGCAGTTGACTAGTAATGCTCTGTCTTTGATTAATCGGATAGCTGATTTGCATTAGCTGTATCTTGAAGTGCACCACCTGTCTTTGATTGAGGAAAAAGACTCAAATTTGACGATGGTAGGTGATTAGTGTGTACTATACGTTGTTGGTGTGTGAGATTTGAAAGTTGGAAGTGTATGTATGATATTTAGGTTAGATGGTGGAGTTTTTGTCATGGTATTTTATACTTGGTAATATATAGGTTTAAGTAGGGTTTACGTGTGGAGGTTGGAGTTATTTTTGCATTATTAATTCCATGGAGTATGTATAATTTTATTGAAGTTATTAGGATTTGTGGGGAGATGGTTTTTATTTAGAGAACCGTAAAAATTAATTTAGATTCGACGTCTATTTAGTCTATAAAGTTCTAAAATAAAATTTGTTTTTTTTAAGGTTTTCAAAA

mRNA sequence

ATGGGTATCTCAAACTCCAAGAAAACTTTGATTCTTTTCCAACTAATTCTCCAATTAACACTAATCCATTCAGTCATAACTCCACAAAGCAGCACAGAGTTCATAAGAAGCTCTTGCAGTTCCACCACTTACCCTCGTCTCTGTTTTTCATCTCTTTCAGTCCATGCCAATGCCATCCAAACAAGTCCCCGCCTTTTGGCCACTGCCGCCCTCTCCGTTTCCCTCTCCTCCGTCAAGTCCACCGCCACTCAAATCCTCAAGCTTTCTCACTCCCATGGCCTCCCCTCCAGAGATGTCTCTGCTCTTGACGACTGTTTGGAAGAACTGAGTGACTCTGTCGACTCCCTCGCTGCTTCCATTTCCGAGATGCCGAAGCTCCGTGGAACCAATTTCGACCTTGCAATGAGTAATGTTCAGACATGGGTTAGCGCCGCTCTGACGGATGAAACCACATGTAGCGAAGGGTTTCAGGGGAAGACGGTGAACGGAGGCGTTAAGGCTGAGGTTAGGACTAAGATTGTCAACATTGCGCAGTTGACTAGTAATGCTCTGTCTTTGATTAATCGGATAGCTGATTTGCATTAG

Coding sequence (CDS)

ATGGGTATCTCAAACTCCAAGAAAACTTTGATTCTTTTCCAACTAATTCTCCAATTAACACTAATCCATTCAGTCATAACTCCACAAAGCAGCACAGAGTTCATAAGAAGCTCTTGCAGTTCCACCACTTACCCTCGTCTCTGTTTTTCATCTCTTTCAGTCCATGCCAATGCCATCCAAACAAGTCCCCGCCTTTTGGCCACTGCCGCCCTCTCCGTTTCCCTCTCCTCCGTCAAGTCCACCGCCACTCAAATCCTCAAGCTTTCTCACTCCCATGGCCTCCCCTCCAGAGATGTCTCTGCTCTTGACGACTGTTTGGAAGAACTGAGTGACTCTGTCGACTCCCTCGCTGCTTCCATTTCCGAGATGCCGAAGCTCCGTGGAACCAATTTCGACCTTGCAATGAGTAATGTTCAGACATGGGTTAGCGCCGCTCTGACGGATGAAACCACATGTAGCGAAGGGTTTCAGGGGAAGACGGTGAACGGAGGCGTTAAGGCTGAGGTTAGGACTAAGATTGTCAACATTGCGCAGTTGACTAGTAATGCTCTGTCTTTGATTAATCGGATAGCTGATTTGCATTAG

Homology

[Close]

BLAST of CSPI03G16010 vs. Swiss-Prot
Match: 21KD_DAUCA (21 kDa protein OS=Daucus carota PE=2 SV=1)

HSP 1 Score: 150.2 bits (378), Expect = 2.3e-35
Identity = 85/192 (44.27%), Postives = 126/192 (65.62%), Query Frame = 1


Query: 6   SKKTLILFQLILQLTLIHSVITPQSSTEFIRSSCSSTTYPRLCFSSLSVHANAIQTSPRL 65
           SK TL+   L++ L    +  +   + +FI++SC+ TTYP +C  SLS +A  IQ +P+ 
Sbjct: 4   SKSTLVFSALLVIL----AAASAAPANQFIKTSCTLTTYPAVCEQSLSAYAKTIQNNPQE 63

Query: 66  LATAALSVSLSSVKSTATQILKLSHSHGLPSRDVSALDDCLEELSDSVDSLAASISEMPK 125
           LA+ AL VSL+  +   T + +L+   GL +R  +A+ DCLEE+ DS+D ++ S  EM  
Sbjct: 64  LASTALQVSLTRTQQAQTFMKRLNKFKGLKARQYAAIHDCLEEVEDSLDRVSRSCDEMKN 123

Query: 126 L---RGTNFDLAMSNVQTWVSAALTDETTCSEGFQGKTVNGGVKAEVRTKIVNIAQLTSN 185
           L   +G +F   MSNV+TWVSAALTDETTC +GF GK ++G +K  VR ++V +A++TSN
Sbjct: 124 LSHAKGNDFTFRMSNVETWVSAALTDETTCMDGFAGKGMDGKIKESVRAQVVAVARVTSN 183

Query: 186 ALSLINRIADLH 195
           AL+L+N  A  H
Sbjct: 184 ALALVNNFAAKH 191

[Close]

BLAST of CSPI03G16010 vs. Swiss-Prot
Match: PME3_PHAVU (Pectinesterase 3 OS=Phaseolus vulgaris GN=MPE3 PE=2 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 2.1e-12
Identity = 56/170 (32.94%), Postives = 87/170 (51.18%), Query Frame = 1


Query: 27  TPQSSTEFIRSSCSSTTYPRLCFSSLSVHANAIQTSPRLLATAALSVSLSSVKSTATQIL 86
           T  S    +++ C +T YP  CFSS+S    +  T P LL   +L V++  + S  +   
Sbjct: 69  TELSPAASLKAVCDTTRYPSSCFSSISSLPESNTTDPELLFKLSLRVAIDELSSFPS--- 128

Query: 87  KLSHSHGLPSRDVSALDDCLEELSDSVDSLAASISEMPKLRGTNFDLA-MSNVQTWVSAA 146
           KL  +    +R   A+D C     D++D L  SIS +  + G     A +SNV+TW+SAA
Sbjct: 129 KLRANAEQDARLQKAIDVCSSVFGDALDRLNDSISALGTVAGRIASSASVSNVETWLSAA 188

Query: 147 LTDETTCSE--GFQGKTVNGGVKAEVRTKIVNIAQLTSNALSLINRIADL 194
           LTD+ TC +  G    T   G   E+ T + N  +  SN+L+++ +I  L
Sbjct: 189 LTDQDTCLDAVGELNSTAARGALQEIETAMRNSTEFASNSLAIVTKILGL 235

[Close]

BLAST of CSPI03G16010 vs. Swiss-Prot
Match: PME2_ARATH (Pectinesterase 2 OS=Arabidopsis thaliana GN=PME2 PE=2 SV=2)

HSP 1 Score: 73.6 bits (179), Expect = 2.8e-12
Identity = 58/206 (28.16%), Postives = 108/206 (52.43%), Query Frame = 1


Query: 6   SKKTLILFQLILQLTLIHSV---------------ITPQSSTE--FIRSSCSSTTYPRLC 65
           + K LIL    + L L+ S+               IT  SST    ++S CSST YP LC
Sbjct: 16  NNKKLILSSAAIALLLLASIVGIAATTTNQNKNQKITTLSSTSHAILKSVCSSTLYPELC 75

Query: 66  FSSLSVHANAIQTSPRLLATAALSVSLSSVKSTATQILKL-SHSHGLPSRDVSALDDCLE 125
           FS+++       TS + +  A+L+++  +VK     + KL +   GL  R+V+AL DCLE
Sbjct: 76  FSAVAATGGKELTSQKEVIEASLNLTTKAVKHNYFAVKKLIAKRKGLTPREVTALHDCLE 135

Query: 126 ELSDSVDSLAASISEMPKL-RGTNFDLAMSNVQTWVSAALTDETTCSEGFQGKTVNGGVK 185
            + +++D L  ++ ++ +  +  +      +++T +S+A+T++ TC +GF     +  V+
Sbjct: 136 TIDETLDELHVAVEDLHQYPKQKSLRKHADDLKTLISSAITNQGTCLDGFSYDDADRKVR 195

Query: 186 AEVRTKIVNIAQLTSNALSLINRIAD 193
             +    V++  + SNAL++I  + +
Sbjct: 196 KALLKGQVHVEHMCSNALAMIKNMTE 221

[Close]

BLAST of CSPI03G16010 vs. Swiss-Prot
Match: PME1_CITSI (Pectinesterase 1 OS=Citrus sinensis GN=PECS-1.1 PE=2 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 6.2e-12
Identity = 48/161 (29.81%), Postives = 91/161 (56.52%), Query Frame = 1


Query: 35  IRSSCSSTTYPRLCFSSLSVHANAIQ--TSPRLLATAALSVSLSSVKSTATQILKLSHSH 94
           ++SSCSST YP LCFS+++    A +  TS + +   +L+++ ++V+     I KL    
Sbjct: 69  LKSSCSSTRYPDLCFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKLLKRT 128

Query: 95  GLPSRDVSALDDCLEELSDSVDSLAASISEMPKLRGT-NFDLAMSNVQTWVSAALTDETT 154
            L  R+  AL DCLE + +++D L  ++ ++ +     +      +++T +SAA+T++ T
Sbjct: 129 NLTKREKVALHDCLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAMTNQGT 188

Query: 155 CSEGFQGKTVNGGVKAEVRTKIVNIAQLTSNALSLINRIAD 193
           C +GF     N  V+  +    V++ ++ SNAL++I  + D
Sbjct: 189 CLDGFSHDDANKHVRDALSDGQVHVEKMCSNALAMIKNMTD 229

[Close]

BLAST of CSPI03G16010 vs. Swiss-Prot
Match: PME3_CITSI (Pectinesterase 3 OS=Citrus sinensis PE=1 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 6.2e-12
Identity = 48/161 (29.81%), Postives = 91/161 (56.52%), Query Frame = 1


Query: 35  IRSSCSSTTYPRLCFSSLSVHANAIQ--TSPRLLATAALSVSLSSVKSTATQILKLSHSH 94
           ++SSCSST YP LCFS+++    A +  TS + +   +L+++ ++V+     I KL    
Sbjct: 69  LKSSCSSTRYPDLCFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKLLKRT 128

Query: 95  GLPSRDVSALDDCLEELSDSVDSLAASISEMPKLRGT-NFDLAMSNVQTWVSAALTDETT 154
            L  R+  AL DCLE + +++D L  ++ ++ +     +      +++T +SAA+T++ T
Sbjct: 129 NLTKREKVALHDCLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAMTNQGT 188

Query: 155 CSEGFQGKTVNGGVKAEVRTKIVNIAQLTSNALSLINRIAD 193
           C +GF     N  V+  +    V++ ++ SNAL++I  + D
Sbjct: 189 CLDGFSHDDANKHVRDALSDGQVHVEKMCSNALAMIKNMTD 229

[Close]

BLAST of CSPI03G16010 vs. TrEMBL
Match: A0A0A0LB27_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G176245 PE=4 SV=1)

HSP 1 Score: 350.9 bits (899), Expect = 1.0e-93
Identity = 190/194 (97.94%), Postives = 191/194 (98.45%), Query Frame = 1


Query: 1   MGISNSKKTLILFQLILQLTLIHSVITPQSSTEFIRSSCSSTTYPRLCFSSLSVHANAIQ 60
           MGISNSKKTLILFQLILQLTLIHS ITPQSSTEFI+SSCSSTTYPRLCFSSLSVHANAIQ
Sbjct: 1   MGISNSKKTLILFQLILQLTLIHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQ 60

Query: 61  TSPRLLATAALSVSLSSVKSTATQILKLSHSHGLPSRDVSALDDCLEELSDSVDSLAASI 120
           TSPRLLATAALSVSLSSVKSTATQILKLSHSHGLPSRDVSALDDCLEELSDSVDSLAASI
Sbjct: 61  TSPRLLATAALSVSLSSVKSTATQILKLSHSHGLPSRDVSALDDCLEELSDSVDSLAASI 120

Query: 121 SEMPKLRGTNFDLAMSNVQTWVSAALTDETTCSEGFQGKTVNGGVKAEVRTKIVNIAQLT 180
           SEMPKLRGTNFDLAMSNVQTWVSAALTDETTCSEGFQGKTVNGGVK  VRTKIVNIAQLT
Sbjct: 121 SEMPKLRGTNFDLAMSNVQTWVSAALTDETTCSEGFQGKTVNGGVKGAVRTKIVNIAQLT 180

Query: 181 SNALSLINRIADLH 195
           SNALSLINRIADLH
Sbjct: 181 SNALSLINRIADLH 194

[Close]

BLAST of CSPI03G16010 vs. TrEMBL
Match: A0A0A0L8D1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G175740 PE=4 SV=1)

HSP 1 Score: 345.9 bits (886), Expect = 3.2e-92
Identity = 187/193 (96.89%), Postives = 189/193 (97.93%), Query Frame = 1


Query: 2   GISNSKKTLILFQLILQLTLIHSVITPQSSTEFIRSSCSSTTYPRLCFSSLSVHANAIQT 61
           GISNSKK+LILFQLILQLTLIHS ITPQSSTEFI+SSCSSTTYPRLCFSSLSVHANAIQT
Sbjct: 44  GISNSKKSLILFQLILQLTLIHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQT 103

Query: 62  SPRLLATAALSVSLSSVKSTATQILKLSHSHGLPSRDVSALDDCLEELSDSVDSLAASIS 121
           SPRLLATAALSVSLSSVKSTATQILKLSHSHGLPSRDVSALDDCLEELSDSVDSLAASIS
Sbjct: 104 SPRLLATAALSVSLSSVKSTATQILKLSHSHGLPSRDVSALDDCLEELSDSVDSLAASIS 163

Query: 122 EMPKLRGTNFDLAMSNVQTWVSAALTDETTCSEGFQGKTVNGGVKAEVRTKIVNIAQLTS 181
           EMPKLRGTNFDLAMSNVQTWVSAALTDETTCSEGFQGKTVNGGVK  VRTKIVNIAQLTS
Sbjct: 164 EMPKLRGTNFDLAMSNVQTWVSAALTDETTCSEGFQGKTVNGGVKGAVRTKIVNIAQLTS 223

Query: 182 NALSLINRIADLH 195
           NALSLINRI DLH
Sbjct: 224 NALSLINRIGDLH 236

[Close]

BLAST of CSPI03G16010 vs. TrEMBL
Match: A0A061G793_THECC (DC1.2-like, putative OS=Theobroma cacao GN=TCM_014946 PE=4 SV=1)

HSP 1 Score: 212.2 bits (539), Expect = 5.5e-52
Identity = 109/169 (64.50%), Postives = 137/169 (81.07%), Query Frame = 1


Query: 26  ITPQSSTEFIRSSCSSTTYPRLCFSSLSVHANAIQTSPRLLATAALSVSLSSVKSTATQI 85
           I PQSS EFIR+SCSSTTYP+LCF +LS HA+ IQTSP+LLA AAL+V+LS+ +ST+  +
Sbjct: 32  IAPQSSAEFIRTSCSSTTYPKLCFETLSTHASLIQTSPQLLAHAALNVTLSTTESTSAMM 91

Query: 86  LKLSHSHGLPSRDVSALDDCLEELSDSVDSLAASISEMPKLRGTNFDLAMSNVQTWVSAA 145
           + LS SHGL  R+  A+ DC+EELSDS+D L  SISEM +++G+NF L +++VQTWVSAA
Sbjct: 92  VTLSKSHGLKPREAEAMQDCVEELSDSIDELRKSISEMGQVKGSNFGLMINDVQTWVSAA 151

Query: 146 LTDETTCSEGFQGKTVNGGVKAEVRTKIVNIAQLTSNALSLINRIADLH 195
           LTDE+TCS+GFQG  +NG VK  VRTKI+ IA LTSNAL+LIN  A LH
Sbjct: 152 LTDESTCSDGFQGNNMNGNVKTAVRTKILRIAHLTSNALALINNYASLH 200

[Close]

BLAST of CSPI03G16010 vs. TrEMBL
Match: M5XKR0_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa011607mg PE=4 SV=1)

HSP 1 Score: 203.4 bits (516), Expect = 2.6e-49
Identity = 112/191 (58.64%), Postives = 144/191 (75.39%), Query Frame = 1


Query: 9   TLILFQLILQLTLIHSV-----ITPQSSTEFIRSSCSSTTYPRLCFSSLSVHANAIQTSP 68
           T  L  L +   +I S      + P+++TEFI++SCS+TTYP+LC +SLS HA+ IQTSP
Sbjct: 12  TCFLTALSISFCIISSTSAARDLAPKTNTEFIKTSCSATTYPKLCLTSLSSHASTIQTSP 71

Query: 69  RLLATAALSVSLSSVKSTATQILKLSHSHGLPSRDVSALDDCLEELSDSVDSLAASISEM 128
           +L+A AAL+V+LSS KST+  +LKLS SHGL  ++V A+ DCLEELSDSVD L +SI+EM
Sbjct: 72  KLMAGAALNVTLSSAKSTSAVMLKLSQSHGLKPKEVGAMRDCLEELSDSVDELQSSIAEM 131

Query: 129 PKLRGTNFDLAMSNVQTWVSAALTDETTCSEGFQGKTVNGGVKAEVRTKIVNIAQLTSNA 188
              +  +F L +S+VQTWVSAALTDE TCSEGF G  +NG +K  VR +IVNIAQLTSNA
Sbjct: 132 GNFKSYDFQLMISDVQTWVSAALTDENTCSEGFGGNGMNGNLKTAVRGRIVNIAQLTSNA 191

Query: 189 LSLINRIADLH 195
           L+LINR A +H
Sbjct: 192 LALINRYASVH 202

[Close]

BLAST of CSPI03G16010 vs. TrEMBL
Match: A0A067JS84_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_26240 PE=4 SV=1)

HSP 1 Score: 199.1 bits (505), Expect = 4.9e-48
Identity = 111/202 (54.95%), Postives = 146/202 (72.28%), Query Frame = 1


Query: 1   MGISNSKKTLILFQLILQLTLIHSV--------ITPQSSTEFIRSSCSSTTYPRLCFSSL 60
           M   +S+ +L  F LI+ +T    V        + P+++TEFIR+SCS+TTYP+LC++SL
Sbjct: 1   MAAFSSQYSLAEFILIIFVTFNFHVNPSKAVRLLDPKTNTEFIRTSCSTTTYPKLCYTSL 60

Query: 61  SVHANAIQTSPRLLATAALSVSLSSVKSTATQILKLSHSHGLPSRDVSALDDCLEELSDS 120
           SVHAN IQTSP+LLA AAL+V+L S KST+  + KLS SHG+   +V A+ DC+EELSD+
Sbjct: 61  SVHANTIQTSPKLLANAALNVTLGSAKSTSAMMSKLSQSHGMKPIEVEAMQDCVEELSDT 120

Query: 121 VDSLAASISEMPKLRGTNFDLAMSNVQTWVSAALTDETTCSEGFQGKTVNGGVKAEVRTK 180
           V+ L  SI EM   + ++F L +++VQTWVSAALTDE TCS+GF GKT+NG  K  VR  
Sbjct: 121 VEGLRKSIGEMKTYKESDFKLMINDVQTWVSAALTDENTCSDGFAGKTMNGNTKTLVREM 180

Query: 181 IVNIAQLTSNALSLINRIADLH 195
           I+NIA LTSNAL+LIN  A LH
Sbjct: 181 ILNIAHLTSNALALINNYASLH 202

[Close]

BLAST of CSPI03G16010 vs. TAIR10
Match: AT5G62360.1 (AT5G62360.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 177.2 bits (448), Expect = 1.0e-44
Identity = 95/184 (51.63%), Postives = 140/184 (76.09%), Query Frame = 1


Query: 15  LILQLTLIHSVITPQSST----EFIRSSCSSTTYPRLCFSSLSVHANAIQTSPRLLATAA 74
           +I  L L+H+  T  ++T    EF++SSC+ TTYPRLCFSSLS HA+ IQTSP+L+A AA
Sbjct: 20  IIAMLKLVHTTTTTTTTTTTNTEFVKSSCTFTTYPRLCFSSLSTHASLIQTSPKLMAHAA 79

Query: 75  LSVSLSSVKSTATQILKLSHSHGLPSRDVSALDDCLEELSDSVDSLAASISEMPKLRGTN 134
           L+++L+S K T+  +++LS+S  L  ++VSA+ DC+EEL D+++ L  SI EM +L G+N
Sbjct: 80  LNITLASAKVTSAMMVRLSNSR-LKPKEVSAMRDCVEELGDTLEELRKSIGEMCQLSGSN 139

Query: 135 FDLAMSNVQTWVSAALTDETTCSEGFQGKTVNGGVKAEVRTKIVNIAQLTSNALSLINRI 194
           +++ +S++QTWVSAALTD  TC++GF+G+ ++G VK  VR +I+ IA LTSNAL+LIN  
Sbjct: 140 YEVYISDIQTWVSAALTDVNTCTDGFEGEDMDGKVKVLVRGRILVIAHLTSNALALINHF 199

[Close]

BLAST of CSPI03G16010 vs. TAIR10
Match: AT5G62350.1 (AT5G62350.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 150.2 bits (378), Expect = 1.3e-36
Identity = 91/189 (48.15%), Postives = 124/189 (65.61%), Query Frame = 1


Query: 11  ILFQLILQLTLIHSVITPQSSTE---FIRSSCSSTTYPRLCFSSLSVHANAIQTSPRLLA 70
           I + L L+LT   +     +S +   FI+SSC +TTYP LC  SLSV+AN IQTSP+ LA
Sbjct: 12  ISYLLSLELTAATAASQTGASKKAINFIQSSCKTTTYPALCVHSLSVYANDIQTSPKRLA 71

Query: 71  TAALSVSLSSVKSTATQILKLSHSHGLPSRDVSALDDCLEELSDSVDSLAASISEMPKLR 130
             A++V+LS  +ST   + +L+   GL  R+V A+ DC+EE++D+VD L  S+ E+ KL 
Sbjct: 72  ETAIAVTLSRAQSTKLFVSRLTRMKGLKKREVEAIKDCVEEMNDTVDRLTKSVQEL-KLC 131

Query: 131 GT-----NFDLAMSNVQTWVSAALTDETTCSEGFQGKTVNGGVKAEVRTKIVNIAQLTSN 190
           G+      F   MSN QTW SAALTDE TCS+GF G+ ++G +K  VR +I+N+   TSN
Sbjct: 132 GSAKDQDQFAYHMSNAQTWTSAALTDENTCSDGFSGRVMDGRIKNSVRARIMNVGHETSN 191

Query: 191 ALSLINRIA 192
           ALSLIN  A
Sbjct: 192 ALSLINAFA 199

[Close]

BLAST of CSPI03G16010 vs. TAIR10
Match: AT4G25260.1 (AT4G25260.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 145.2 bits (365), Expect = 4.2e-35
Identity = 89/198 (44.95%), Postives = 126/198 (63.64%), Query Frame = 1


Query: 5   NSKKTLILFQLILQLTLIHSV--ITPQSS--TEFIRSSCSSTTYPRLCFSSLSVHANAIQ 64
           N + +LILF L L    I     +  +SS  TEFI++SC +T+YP  CF SLS +A+ I+
Sbjct: 4   NFELSLILFVLYLSTAAIVMARNLEEESSGDTEFIKASCETTSYPDRCFQSLSSYASEIK 63

Query: 65  TSPRLLATAALSVSLSSVKSTATQILKLSHSHGLPSRDVSALDDCLEELSDSVDSLAASI 124
             PR LA  AL+VS++  KS  T + +++   G+  R   A+ DCLEE+ D+VD L+ S+
Sbjct: 64  KQPRKLAETALAVSIARAKSAKTYVSEMTDYKGITKRQHEAVADCLEEMGDTVDRLSNSL 123

Query: 125 SEMPKLR----GTNFDLAMSNVQTWVSAALTDETTCSEGFQGKTVNGGVKAEVRTKIVNI 184
            E+  L     G +F   +SNV+TW SAALTDET C +GF GK + G +K+ +RT IV++
Sbjct: 124 KELKHLEEGDSGEDFWFCLSNVRTWTSAALTDETACMDGFGGKAMAGELKSLIRTHIVSV 183

Query: 185 AQLTSNALSLINRIADLH 195
           A+ TSNAL+LIN  A  H
Sbjct: 184 AEETSNALALINDFASKH 201

[Close]

BLAST of CSPI03G16010 vs. TAIR10
Match: AT3G47380.1 (AT3G47380.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 137.5 bits (345), Expect = 8.8e-33
Identity = 85/193 (44.04%), Postives = 122/193 (63.21%), Query Frame = 1


Query: 9   TLILFQLILQLTLIHS-----VITPQSSTEFIRSSCSSTTYPRLCFSSLSVHANAIQTSP 68
           TL LF L   +    S      IT + +  FI++SC +TTYP +C +SL+ +AN+IQTSP
Sbjct: 8   TLFLFLLSTAILTASSSAPRAAITSKRAINFIQASCKATTYPTVCVNSLTGYANSIQTSP 67

Query: 69  RLLATAALSVSLSSVKSTATQILKLSHSHGLPSRDVSALDDCLEELSDSVDSLAASISEM 128
           R LA  AL+V+++  +ST   + +L     L  R++ A+ DC+EE+ D+VD L  SI E+
Sbjct: 68  RRLAETALNVTVTQAQSTKVFVWRLGRFTSLKKREIQAVKDCIEEIHDAVDRLTMSIHEV 127

Query: 129 PKLRGT-----NFDLAMSNVQTWVSAALTDETTCSEGFQGKTVNGGVKAEVRTKIVNIAQ 188
            K+ G+      F   MSN QTW SAALT+  TCS+GF G+ ++G VK  VR +I+N+ +
Sbjct: 128 -KMCGSAKGRDQFWFHMSNAQTWTSAALTNANTCSDGFAGRVMDGRVKNSVRARILNLGR 187

Query: 189 LTSNALSLINRIA 192
            TSNAL+LIN  A
Sbjct: 188 GTSNALALINAFA 199

[Close]

BLAST of CSPI03G16010 vs. TAIR10
Match: AT1G62760.1 (AT1G62760.1 Plant invertase/pectin methylesterase inhibitor superfamily protein)

HSP 1 Score: 128.3 bits (321), Expect = 5.3e-30
Identity = 74/171 (43.27%), Postives = 114/171 (66.67%), Query Frame = 1


Query: 24  SVITPQSSTEFIRSSCSSTTYPRLCFSSLSVHANAIQTSPRLLATAALSVSLSSVKSTAT 83
           S  + Q++ ++I++SC+ T Y  +C++SLS +A+ I+++P+ LA  AL+++LSS KS + 
Sbjct: 137 STYSNQTNLDYIKTSCNITLYKTICYNSLSPYASTIRSNPQKLAVIALNLTLSSAKSASK 196

Query: 84  QILKLSHSHGLPSRDVSALDDCLEELSDSVDSLAASISEMPKLR---GTNFDLAMSNVQT 143
            +  +SH  GL   +V A+ DC+EE+ DSV SL  SI E+  +       F++ MS+V+T
Sbjct: 197 FVKNISHGGGLTRLEVVAVADCVEEIGDSVTSLQDSIRELDSINYKDSAKFEMVMSDVET 256

Query: 144 WVSAALTDETTCSEGFQGKTVNGGVKAEVRTKIVNIAQLTSNALSLINRIA 192
           WVSAALT++ TC +GF    V   VK  VR  +V +A+LTSNAL+LIN  A
Sbjct: 257 WVSAALTNDDTCMDGF--SLVKTAVKDLVRRHVVEVARLTSNALALINMYA 305

[Close]

BLAST of CSPI03G16010 vs. NCBI nr
Match: gi|778679325|ref|XP_004148138.2| (PREDICTED: 21 kDa protein [Cucumis sativus])

HSP 1 Score: 350.9 bits (899), Expect = 1.4e-93
Identity = 190/194 (97.94%), Postives = 191/194 (98.45%), Query Frame = 1


Query: 1   MGISNSKKTLILFQLILQLTLIHSVITPQSSTEFIRSSCSSTTYPRLCFSSLSVHANAIQ 60
           MGISNSKKTLILFQLILQLTLIHS ITPQSSTEFI+SSCSSTTYPRLCFSSLSVHANAIQ
Sbjct: 1   MGISNSKKTLILFQLILQLTLIHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQ 60

Query: 61  TSPRLLATAALSVSLSSVKSTATQILKLSHSHGLPSRDVSALDDCLEELSDSVDSLAASI 120
           TSPRLLATAALSVSLSSVKSTATQILKLSHSHGLPSRDVSALDDCLEELSDSVDSLAASI
Sbjct: 61  TSPRLLATAALSVSLSSVKSTATQILKLSHSHGLPSRDVSALDDCLEELSDSVDSLAASI 120

Query: 121 SEMPKLRGTNFDLAMSNVQTWVSAALTDETTCSEGFQGKTVNGGVKAEVRTKIVNIAQLT 180
           SEMPKLRGTNFDLAMSNVQTWVSAALTDETTCSEGFQGKTVNGGVK  VRTKIVNIAQLT
Sbjct: 121 SEMPKLRGTNFDLAMSNVQTWVSAALTDETTCSEGFQGKTVNGGVKGAVRTKIVNIAQLT 180

Query: 181 SNALSLINRIADLH 195
           SNALSLINRIADLH
Sbjct: 181 SNALSLINRIADLH 194

[Close]

BLAST of CSPI03G16010 vs. NCBI nr
Match: gi|778688522|ref|XP_011652768.1| (PREDICTED: 21 kDa protein-like [Cucumis sativus])

HSP 1 Score: 345.9 bits (886), Expect = 4.6e-92
Identity = 187/193 (96.89%), Postives = 189/193 (97.93%), Query Frame = 1


Query: 2   GISNSKKTLILFQLILQLTLIHSVITPQSSTEFIRSSCSSTTYPRLCFSSLSVHANAIQT 61
           GISNSKK+LILFQLILQLTLIHS ITPQSSTEFI+SSCSSTTYPRLCFSSLSVHANAIQT
Sbjct: 44  GISNSKKSLILFQLILQLTLIHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQT 103

Query: 62  SPRLLATAALSVSLSSVKSTATQILKLSHSHGLPSRDVSALDDCLEELSDSVDSLAASIS 121
           SPRLLATAALSVSLSSVKSTATQILKLSHSHGLPSRDVSALDDCLEELSDSVDSLAASIS
Sbjct: 104 SPRLLATAALSVSLSSVKSTATQILKLSHSHGLPSRDVSALDDCLEELSDSVDSLAASIS 163

Query: 122 EMPKLRGTNFDLAMSNVQTWVSAALTDETTCSEGFQGKTVNGGVKAEVRTKIVNIAQLTS 181
           EMPKLRGTNFDLAMSNVQTWVSAALTDETTCSEGFQGKTVNGGVK  VRTKIVNIAQLTS
Sbjct: 164 EMPKLRGTNFDLAMSNVQTWVSAALTDETTCSEGFQGKTVNGGVKGAVRTKIVNIAQLTS 223

Query: 182 NALSLINRIADLH 195
           NALSLINRI DLH
Sbjct: 224 NALSLINRIGDLH 236

[Close]

BLAST of CSPI03G16010 vs. NCBI nr
Match: gi|590671750|ref|XP_007038418.1| (DC1.2-like, putative [Theobroma cacao])

HSP 1 Score: 212.2 bits (539), Expect = 7.9e-52
Identity = 109/169 (64.50%), Postives = 137/169 (81.07%), Query Frame = 1


Query: 26  ITPQSSTEFIRSSCSSTTYPRLCFSSLSVHANAIQTSPRLLATAALSVSLSSVKSTATQI 85
           I PQSS EFIR+SCSSTTYP+LCF +LS HA+ IQTSP+LLA AAL+V+LS+ +ST+  +
Sbjct: 32  IAPQSSAEFIRTSCSSTTYPKLCFETLSTHASLIQTSPQLLAHAALNVTLSTTESTSAMM 91

Query: 86  LKLSHSHGLPSRDVSALDDCLEELSDSVDSLAASISEMPKLRGTNFDLAMSNVQTWVSAA 145
           + LS SHGL  R+  A+ DC+EELSDS+D L  SISEM +++G+NF L +++VQTWVSAA
Sbjct: 92  VTLSKSHGLKPREAEAMQDCVEELSDSIDELRKSISEMGQVKGSNFGLMINDVQTWVSAA 151

Query: 146 LTDETTCSEGFQGKTVNGGVKAEVRTKIVNIAQLTSNALSLINRIADLH 195
           LTDE+TCS+GFQG  +NG VK  VRTKI+ IA LTSNAL+LIN  A LH
Sbjct: 152 LTDESTCSDGFQGNNMNGNVKTAVRTKILRIAHLTSNALALINNYASLH 200

[Close]

BLAST of CSPI03G16010 vs. NCBI nr
Match: gi|659133745|ref|XP_008466883.1| (PREDICTED: 21 kDa protein-like, partial [Cucumis melo])

HSP 1 Score: 205.7 bits (522), Expect = 7.4e-50
Identity = 114/122 (93.44%), Postives = 118/122 (96.72%), Query Frame = 1


Query: 1   MGISNSKKTLILFQLILQLTLIHSVITPQSSTEFIRSSCSSTTYPRLCFSSLSVHANAIQ 60
           MGISN KK+LILFQLILQLT+IHS ITPQSSTEFI+SSCSSTTYPRLCFSSLSVHANAIQ
Sbjct: 1   MGISNCKKSLILFQLILQLTIIHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQ 60

Query: 61  TSPRLLATAALSVSLSSVKSTATQILKLSHSHGLPSRDVSALDDCLEELSDSVDSLAASI 120
           TSPRLLATAALSVSLSSVKST TQILKLSHSHGL SRDVSAL+DCLEELSDSVDSLAASI
Sbjct: 61  TSPRLLATAALSVSLSSVKSTTTQILKLSHSHGLSSRDVSALNDCLEELSDSVDSLAASI 120

Query: 121 SE 123
           SE
Sbjct: 121 SE 122

[Close]

BLAST of CSPI03G16010 vs. NCBI nr
Match: gi|1009122319|ref|XP_015877940.1| (PREDICTED: 21 kDa protein-like [Ziziphus jujuba])

HSP 1 Score: 203.8 bits (517), Expect = 2.8e-49
Identity = 109/201 (54.23%), Postives = 155/201 (77.11%), Query Frame = 1


Query: 1   MGISNSK--KTLILFQLILQLTLIHSVIT-----PQSSTEFIRSSCSSTTYPRLCFSSLS 60
           M  + SK   T +L  + + L++ HS        P+++T+FI++SCS T+YP+LCF+SLS
Sbjct: 1   MAFTTSKLFSTFLLLDIFISLSM-HSTSAARNPLPKTNTDFIKTSCSVTSYPKLCFTSLS 60

Query: 61  VHANAIQTSPRLLATAALSVSLSSVKSTATQILKLSHSHGLPSRDVSALDDCLEELSDSV 120
            HANAIQ SP+LLA  AL+V+LSS KST+  ++ LS S G+  R+V+A+ DC+EELSDSV
Sbjct: 61  AHANAIQASPQLLAGTALNVTLSSAKSTSNLMVLLSRSQGINPREVAAMKDCIEELSDSV 120

Query: 121 DSLAASISEMPKLRGTNFDLAMSNVQTWVSAALTDETTCSEGFQGKTVNGGVKAEVRTKI 180
           D L  SI+EM +L+GTNF+L ++++QTWVSAALTDE+TC++GF GK++NG +K  V+ +I
Sbjct: 121 DELGKSINEMGQLKGTNFNLVINDIQTWVSAALTDESTCTDGFAGKSMNGNLKITVKDRI 180

Query: 181 VNIAQLTSNALSLINRIADLH 195
           VN+AQLTSNAL+LIN+ A +H
Sbjct: 181 VNVAQLTSNALALINKFALIH 200

The following BLAST results are available for this feature:

BLAST of CSPI03G16010 vs. Swiss-Prot


Match Name	E-value	Identity	Description
21KD_DAUCA	2.3e-35	44.27	21 kDa protein OS=Daucus carota PE=2 SV=1	[more]
PME3_PHAVU	2.1e-12	32.94	Pectinesterase 3 OS=Phaseolus vulgaris GN=MPE3 PE=2 SV=1	[more]
PME2_ARATH	2.8e-12	28.16	Pectinesterase 2 OS=Arabidopsis thaliana GN=PME2 PE=2 SV=2	[more]
PME1_CITSI	6.2e-12	29.81	Pectinesterase 1 OS=Citrus sinensis GN=PECS-1.1 PE=2 SV=1	[more]
PME3_CITSI	6.2e-12	29.81	Pectinesterase 3 OS=Citrus sinensis PE=1 SV=1	[more]

BLAST of CSPI03G16010 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LB27_CUCSA	1.0e-93	97.94	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G176245 PE=4 SV=1	[more]
A0A0A0L8D1_CUCSA	3.2e-92	96.89	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G175740 PE=4 SV=1	[more]
A0A061G793_THECC	5.5e-52	64.50	DC1.2-like, putative OS=Theobroma cacao GN=TCM_014946 PE=4 SV=1	[more]
M5XKR0_PRUPE	2.6e-49	58.64	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa011607mg PE=4 SV=1	[more]
A0A067JS84_JATCU	4.9e-48	54.95	Uncharacterized protein OS=Jatropha curcas GN=JCGZ_26240 PE=4 SV=1	[more]

BLAST of CSPI03G16010 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G62360.1	1.0e-44	51.63	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G62350.1	1.3e-36	48.15	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT4G25260.1	4.2e-35	44.95	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT3G47380.1	8.8e-33	44.04	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT1G62760.1	5.3e-30	43.27	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]

BLAST of CSPI03G16010 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778679325\|ref\|XP_004148138.2\|	1.4e-93	97.94	PREDICTED: 21 kDa protein [Cucumis sativus]	[more]
gi\|778688522\|ref\|XP_011652768.1\|	4.6e-92	96.89	PREDICTED: 21 kDa protein-like [Cucumis sativus]	[more]
gi\|590671750\|ref\|XP_007038418.1\|	7.9e-52	64.50	DC1.2-like, putative [Theobroma cacao]	[more]
gi\|659133745\|ref\|XP_008466883.1\|	7.4e-50	93.44	PREDICTED: 21 kDa protein-like, partial [Cucumis melo]	[more]
gi\|1009122319\|ref\|XP_015877940.1\|	2.8e-49	54.23	PREDICTED: 21 kDa protein-like [Ziziphus jujuba]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR006501	Pectinesterase_inhib_dom

Vocabulary: **Molecular Function**
Term	Definition
GO:0004857	enzyme inhibitor activity

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
biological_process	GO:0005982	starch metabolic process
biological_process	GO:0005985	sucrose metabolic process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004857	enzyme inhibitor activity
molecular_function	GO:0030599	pectinesterase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G16010.1	CSPI03G16010.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	GENE3D	G3DSA:1.20.140.40		coord: 31..190 score: 3.7
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 34..186 score: 3.1
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 30..186 score: 4.0
IPR006501	Pectinesterase inhibitor domain	TIGRFAMs	TIGR01614	TIGR01614	coord: 8..189 score: 2.0
IPR006501	Pectinesterase inhibitor domain	unknown	SSF101148	Plant invertase/pectin methylesterase inhibitor	coord: 33..190 score: 3.92
None	No IPR available	PANTHER	PTHR31080	FAMILY NOT NAMED	coord: 1..194 score: 2.3
None	No IPR available	PANTHER	PTHR31080:SF19	PLANT INVERTASE/PECTIN METHYLESTERASE INHIBITOR SUPERFAMILY PROTEIN	coord: 1..194 score: 2.3

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CSPI03G16010	ClCG05G007410	Watermelon (Charleston Gray)	cpiwcgB226

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CSPI03G16010	Cucumber (Chinese Long) v3	cpicucB144
CSPI03G16010	Cucumber (Chinese Long) v3	cpicucB169
CSPI03G16010	Watermelon (97103) v2	cpiwmbB181
CSPI03G16010	Watermelon (97103) v2	cpiwmbB209
CSPI03G16010	Wax gourd	cpiwgoB269
CSPI03G16010	Wild cucumber (PI 183967)	cpicpiB116
CSPI03G16010	Cucumber (Gy14) v1	cgycpiB383
CSPI03G16010	Cucumber (Gy14) v1	cgycpiB453
CSPI03G16010	Cucurbita maxima (Rimu)	cmacpiB286
CSPI03G16010	Cucurbita maxima (Rimu)	cmacpiB335
CSPI03G16010	Cucurbita maxima (Rimu)	cmacpiB601
CSPI03G16010	Cucurbita maxima (Rimu)	cmacpiB609
CSPI03G16010	Cucurbita maxima (Rimu)	cmacpiB712
CSPI03G16010	Cucurbita moschata (Rifu)	cmocpiB276
CSPI03G16010	Cucurbita moschata (Rifu)	cmocpiB325
CSPI03G16010	Cucurbita moschata (Rifu)	cmocpiB589
CSPI03G16010	Cucurbita moschata (Rifu)	cmocpiB598
CSPI03G16010	Cucurbita moschata (Rifu)	cmocpiB704
CSPI03G16010	Cucumber (Chinese Long) v2	cpicuB120
CSPI03G16010	Cucumber (Chinese Long) v2	cpicuB141
CSPI03G16010	Melon (DHL92) v3.5.1	cpimeB163
CSPI03G16010	Melon (DHL92) v3.5.1	cpimeB230
CSPI03G16010	Watermelon (Charleston Gray)	cpiwcgB210
CSPI03G16010	Watermelon (97103) v1	cpiwmB231
CSPI03G16010	Watermelon (97103) v1	cpiwmB261
CSPI03G16010	Cucurbita pepo (Zucchini)	cpecpiB171
CSPI03G16010	Cucurbita pepo (Zucchini)	cpecpiB217
CSPI03G16010	Cucurbita pepo (Zucchini)	cpecpiB393
CSPI03G16010	Cucurbita pepo (Zucchini)	cpecpiB721
CSPI03G16010	Cucurbita pepo (Zucchini)	cpecpiB725
CSPI03G16010	Bottle gourd (USVL1VR-Ls)	cpilsiB155
CSPI03G16010	Bottle gourd (USVL1VR-Ls)	cpilsiB208
CSPI03G16010	Melon (DHL92) v3.6.1	cpimedB154
CSPI03G16010	Melon (DHL92) v3.6.1	cpimedB224
CSPI03G16010	Cucumber (Gy14) v2	cgybcpiB110
CSPI03G16010	Cucumber (Gy14) v2	cgybcpiB213
CSPI03G16010	Silver-seed gourd	carcpiB0406
CSPI03G16010	Silver-seed gourd	carcpiB0905
CSPI03G16010	Silver-seed gourd	carcpiB0955