CSPI03G10870 (gene) Wild cucumber (PI 183967) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CSPI03G10870
Type	gene
Organism	Cucumis sativus (Wild cucumber (PI 183967))
Description	Papain-like cysteine proteinase
Location	Chr3 : 8578308 .. 8579067 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTGGAACGTTGCCAAGGTATTTGTCTATTACCGCCGAATGTGGCGCATCAAACATCCACACATCTTCCAAACACGTGTAAGTATGTTTTCTAAATAATATGCTTATAATATATACATGTTTGTCTATTGATTTTGTTAGGATGGTTTGCCCAAGAAAGTGGATTGGGTTGAGAGCGCTGTTGTTACTCCGCCAAGAGATCAAGGACCCACTCCAACTTGTTGGGCATATAGTGGAGTAGCAGCAATAGAATCGATGAATAAAATAAAAAGAGGGCAATTAGTTAACATGACCGTTGTTGATGTTATTATTGACAACATACGGTTTTGGATGGATGGAGCATGGCCGGACGTTGTATTTCACGATGGAGTAAAGCAAGGCATTCACAAGCTCAAAAGAAAGGATGTATGTTTTTTTGTTTTTGTTTTTTAAATTTATTTATCCAATATTTATATTTATATATTTATTTATAGAGGTGGTAAGGATAGATGACTACAACATTGTACGTCCAAGTGAGTGGGCTTTAAAGTCCTTGTTAAGCAAACACCCAGTTTGTATCAGCATTTCTGTTGATGAATATGGTATGTTTAGAAATTACAGAGGCAGACTTTACTATGGTCCGTTCGGAGAAATAGCAAATCATTCCATGTTAGCAGTAGGGTATAGTAAAGACTACTCGCTGCTCAAAAACTCATGGGGATGTATCTGGGGAGAAAATGGATATTGCAGAATCGCCACTCAACAAGTATTTAGGAAATAA

mRNA sequence

ATGTGGAACGTTGCCAAGGATGGTTTGCCCAAGAAAGTGGATTGGGTTGAGAGCGCTGTTGTTACTCCGCCAAGAGATCAAGGACCCACTCCAACTTGTTGGGCATATAGTGGAGTAGCAGCAATAGAATCGATGAATAAAATAAAAAGAGGGCAATTAGTTAACATGACCGTTGTTGATGTTATTATTGACAACATACGGTTTTGGATGGATGGAGCATGGCCGGACGTTGTATTTCACGATGGAGTAAAGCAAGGCATTCACAAGCTCAAAAGAAAGGATTCCTTGTTAAGCAAACACCCAGTTTGTATCAGCATTTCTGTTGATGAATATGGTATGTTTAGAAATTACAGAGGCAGACTTTACTATGGTCCGTTCGGAGAAATAGCAAATCATTCCATGTTAGCAGTAGGGTATAGTAAAGACTACTCGCTGCTCAAAAACTCATGGGGATGTATCTGGGGAGAAAATGGATATTGCAGAATCGCCACTCAACAAGTATTTAGGAAATAA

Coding sequence (CDS)

ATGTGGAACGTTGCCAAGGATGGTTTGCCCAAGAAAGTGGATTGGGTTGAGAGCGCTGTTGTTACTCCGCCAAGAGATCAAGGACCCACTCCAACTTGTTGGGCATATAGTGGAGTAGCAGCAATAGAATCGATGAATAAAATAAAAAGAGGGCAATTAGTTAACATGACCGTTGTTGATGTTATTATTGACAACATACGGTTTTGGATGGATGGAGCATGGCCGGACGTTGTATTTCACGATGGAGTAAAGCAAGGCATTCACAAGCTCAAAAGAAAGGATTCCTTGTTAAGCAAACACCCAGTTTGTATCAGCATTTCTGTTGATGAATATGGTATGTTTAGAAATTACAGAGGCAGACTTTACTATGGTCCGTTCGGAGAAATAGCAAATCATTCCATGTTAGCAGTAGGGTATAGTAAAGACTACTCGCTGCTCAAAAACTCATGGGGATGTATCTGGGGAGAAAATGGATATTGCAGAATCGCCACTCAACAAGTATTTAGGAAATAA

Homology

[Close]

BLAST of CSPI03G10870 vs. Swiss-Prot
Match: PAPA3_CARPA (Caricain OS=Carica papaya PE=1 SV=2)

HSP 1 Score: 104.4 bits (259), Expect = 1.3e-21
Identity = 64/195 (32.82%), Postives = 92/195 (47.18%), Query Frame = 1


Query: 9   LPKKVDWVESAVVTPPRDQGPTPTCWAYSGVAAIESMNKIKRGQLVNMTVVDVI-IDNIR 68
           LP+ VDW +   VTP R QG   +CWA+S VA +E +NKI+ G+LV ++  +++  +   
Sbjct: 133 LPENVDWRKKGAVTPVRHQGSCGSCWAFSAVATVEGINKIRTGKLVELSEQELVDCERRS 192

Query: 69  FWMDGAWPDVVFHDGVKQGIH-----KLKRKD---------------------------- 128
               G +P        K GIH       K K                             
Sbjct: 193 HGCKGGYPPYALEYVAKNGIHLRSKYPYKAKQGTCRAKQVGGPIVKTSGVGRVQPNNEGN 252

Query: 129 --SLLSKHPVCISISVDEYGM-FRNYRGRLYYGPFGEIANHSMLAVGYSKD----YSLLK 163
             + ++K PV  S+ V+  G  F+ Y+G ++ GP G   +H++ AVGY K     Y L+K
Sbjct: 253 LLNAIAKQPV--SVVVESKGRPFQLYKGGIFEGPCGTKVDHAVTAVGYGKSGGKGYILIK 312

[Close]

BLAST of CSPI03G10870 vs. Swiss-Prot
Match: ANAN_ANACO (Ananain OS=Ananas comosus GN=AN1 PE=1 SV=2)

HSP 1 Score: 100.1 bits (248), Expect = 2.4e-20
Identity = 51/193 (26.42%), Postives = 98/193 (50.78%), Query Frame = 1


Query: 9   LPKKVDWVESAVVTPPRDQGPTPTCWAYSGVAAIESMNKIKRGQLVNMTVVDVIIDNIRF 68
           +P+ +DW +S  VT  ++QG   +CWA++ +A +ES+ KIKRG LV+++   V+   + +
Sbjct: 123 VPQSIDWRDSGAVTSVKNQGRCGSCWAFASIATVESIYKIKRGNLVSLSEQQVLDCAVSY 182

Query: 69  -----WMDGAWPDVVFHDGVKQG----------------------------IHKLKRKDS 128
                W++ A+  ++ + GV                               + +   ++ 
Sbjct: 183 GCKGGWINKAYSFIISNKGVASAAIYPYKAAKGTCKTNGVPNSAYITRYTYVQRNNERNM 242

Query: 129 LLSKHPVCISISVDEYGMFRNYRGRLYYGPFGEIANHSMLAVGYSKDYS-----LLKNSW 164
           + +     I+ ++D  G F++Y+  ++ GP G   NH+++ +GY +D S     +++NSW
Sbjct: 243 MYAVSNQPIAAALDASGNFQHYKRGVFTGPCGTRLNHAIVIIGYGQDSSGKKFWIVRNSW 302

[Close]

BLAST of CSPI03G10870 vs. Swiss-Prot
Match: RDL6_ARATH (Probable cysteine protease RDL6 OS=Arabidopsis thaliana GN=RDL6 PE=3 SV=1)

HSP 1 Score: 95.9 bits (237), Expect = 4.6e-19
Identity = 61/202 (30.20%), Postives = 100/202 (49.50%), Query Frame = 1


Query: 4   VAKDGLPKKVDWVESAVVTPPRDQGPTPTCWAYSGVAAIESMNKIKRGQLVNMTVVDVII 63
           +A D LP+ VDW +   V+  +DQG   +CWA+S VAA+E +NKI  G+L++++  +++ 
Sbjct: 128 LAGDQLPESVDWRQEGAVSEIKDQGTCNSCWAFSTVAAVEGLNKIVTGELISLSEQELVD 187

Query: 64  DNI-------RFWMDGAWPDVVFHDGVK-------QGIH-KLKRKDSLLSKHPVC----- 123
            N+          MD A+  ++ ++G+        QG      RK S  +K         
Sbjct: 188 CNLVNNGCYGSGLMDTAFQFLINNNGLDSEKDYPYQGTQGSCNRKQSTSNKVITIDSYED 247

Query: 124 -----------------ISISVDEYGM-FRNYRGRLYYGPFGEIANHSMLAVGY----SK 164
                            +S+ VD+    F  YR  +Y GP G   +H+++ VGY     +
Sbjct: 248 VPANDEISLQKAVAHQPVSVGVDKKSQEFMLYRSCIYNGPCGTNLDHALVIVGYGSENGQ 307

[Close]

BLAST of CSPI03G10870 vs. Swiss-Prot
Match: MDO1_PSEMR (Macrodontain-1 OS=Pseudananas macrodontes PE=1 SV=1)

HSP 1 Score: 95.1 bits (235), Expect = 7.8e-19
Identity = 52/189 (27.51%), Postives = 94/189 (49.74%), Query Frame = 1


Query: 9   LPKKVDWVESAVVTPPRDQGPTPTCWAYSGVAAIESMNKIKRGQLVNMTVVDVIIDNIRF 68
           +P+ +DW +   V   ++QGP   CWA++ +A +E + KI++G LV ++  +V+   + +
Sbjct: 2   VPQSIDWRDYGAVNEVKNQGPCGGCWAFAAIATVEGIYKIRKGNLVYLSEQEVLDCAVSY 61

Query: 69  -----WMDGAWPDVVFHDGVKQ-------------------------GIHKLKRKDSLLS 128
                W++ A+  ++ ++GV                           G   ++R D    
Sbjct: 62  GCKGGWVNRAYDFIISNNGVTTDENYPYRAYQGTCNANYFPNSAYITGYSYVRRNDESHM 121

Query: 129 KHPVC---ISISVDEYG-MFRNYRGRLYYGPFGEIANHSMLAVGYSKD-YSLLKNSWGCI 163
            + V    I+  +D  G  F+ Y+G +Y GP G   NH++  +GY +D Y +++NSWG  
Sbjct: 122 MYAVSNQPIAALIDASGDNFQYYKGGVYSGPCGFSLNHAITIIGYGRDSYWIVRNSWGSS 181

[Close]

BLAST of CSPI03G10870 vs. Swiss-Prot
Match: BROM1_ANACO (Fruit bromelain OS=Ananas comosus PE=1 SV=1)

HSP 1 Score: 94.0 bits (232), Expect = 1.7e-18
Identity = 51/195 (26.15%), Postives = 93/195 (47.69%), Query Frame = 1


Query: 9   LPKKVDWVESAVVTPPRDQGPTPTCWAYSGVAAIESMNKIKRGQLVNMTVVDVIIDNIRF 68
           +P+ +DW +   V   ++Q P  +CW+++ +A +E + KIK G LV+++  +V+   + +
Sbjct: 123 VPQSIDWRDYGAVNEVKNQNPCGSCWSFAAIATVEGIYKIKTGYLVSLSEQEVLDCAVSY 182

Query: 69  -----WMDGAWPDVVFHDGVKQ-------------------------GIHKLKRKDS--- 128
                W++ A+  ++ ++GV                           G   ++R D    
Sbjct: 183 GCKGGWVNKAYDFIISNNGVTTEENYPYLAYQGTCNANSFPNSAYITGYSYVRRNDERSM 242

Query: 129 --LLSKHPVCISISVDEYGMFRNYRGRLYYGPFGEIANHSMLAVGYSKD-----YSLLKN 164
              +S  P+   I   E   F+ Y G ++ GP G   NH++  +GY +D     Y +++N
Sbjct: 243 MYAVSNQPIAALIDASEN--FQYYNGGVFSGPCGTSLNHAITIIGYGQDSSGTKYWIVRN 302

[Close]

BLAST of CSPI03G10870 vs. TrEMBL
Match: A0A0A0L7Y4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G131900 PE=4 SV=1)

HSP 1 Score: 162.2 bits (409), Expect = 5.8e-37
Identity = 76/91 (83.52%), Postives = 80/91 (87.91%), Query Frame = 1


Query: 1  MWNV-------AKDGLPKKVDWVESAVVTPPRDQGPTPTCWAYSGVAAIESMNKIKRGQL 60
          MW +        +DGLPKKVDWVESA VTPPRDQGP+PTC AYSGVAAIESMNKIKRGQL
Sbjct: 1  MWRIKHPHIFQTRDGLPKKVDWVESAAVTPPRDQGPSPTCRAYSGVAAIESMNKIKRGQL 60

Query: 61 VNMTVVDVIIDNIRFWMDGAWPDVVFHDGVK 85
          VN+TVVDVIIDNIRFWMDGAWPDVVFHDGVK
Sbjct: 61 VNLTVVDVIIDNIRFWMDGAWPDVVFHDGVK 91

[Close]

BLAST of CSPI03G10870 vs. TrEMBL
Match: E5LBE8_VASCU (Mitogenic proteinase (Fragment) OS=Vasconcellea cundinamarcensis PE=2 SV=1)

HSP 1 Score: 108.2 bits (269), Expect = 9.9e-21
Identity = 59/189 (31.22%), Postives = 90/189 (47.62%), Query Frame = 1


Query: 10  PKKVDWVESAVVTPPRDQGPTPTCWAYSGVAAIESMNKIKRGQLVNMTVVDVI-IDNIRF 69
           P+ +DW +   VTP +DQ P  +CWA+S VA +E +NKI  G+L++++  +++  D    
Sbjct: 2   PESIDWRQKGAVTPVKDQNPCGSCWAFSTVATVEGINKIVTGKLISLSEQELLDCDRRSH 61

Query: 70  WMDGAWPDVVFHDGVKQGIH------------KLKRKDS--------------------- 129
             +G +        V  G+H              + KD                      
Sbjct: 62  GCNGGYQTTSLQYVVDNGVHTEYEYPYEKKQGNCRAKDKKGLKVQITGYKRVPPNDEISL 121

Query: 130 --LLSKHPVCISISVDEYGMFRNYRGRLYYGPFGEIANHSMLAVGYSKDYSLLKNSWGCI 163
             +++  PV + I   +   F  YRG +Y GP G   +H++ A+GY KDY L+KNSWG  
Sbjct: 122 IKVIANQPVSVLIESKDRS-FHFYRGGIYKGPCGTRLDHAVTAIGYGKDYILIKNSWGPN 181

[Close]

BLAST of CSPI03G10870 vs. TrEMBL
Match: Q42673_CARPA (Papaya proteinase omega OS=Carica papaya GN=omegaii PE=2 SV=1)

HSP 1 Score: 104.4 bits (259), Expect = 1.4e-19
Identity = 64/195 (32.82%), Postives = 92/195 (47.18%), Query Frame = 1


Query: 9   LPKKVDWVESAVVTPPRDQGPTPTCWAYSGVAAIESMNKIKRGQLVNMTVVDVI-IDNIR 68
           LP+ VDW +   VTP R QG   +CWA+S VA +E +NKI+ G+LV ++  +++  +   
Sbjct: 133 LPENVDWRKKGAVTPVRHQGSCGSCWAFSAVATVEGINKIRTGKLVELSEQELVDCERRS 192

Query: 69  FWMDGAWPDVVFHDGVKQGIH-----KLKRKD---------------------------- 128
               G +P        K GIH       K K                             
Sbjct: 193 HGCKGGYPPYALEYVAKNGIHLRSKYPYKAKQGTCRAKQVGGPIVKTSGVGRVQPNNEGN 252

Query: 129 --SLLSKHPVCISISVDEYGM-FRNYRGRLYYGPFGEIANHSMLAVGYSKD----YSLLK 163
             + ++K PV  S+ V+  G  F+ Y+G ++ GP G   +H++ AVGY K     Y L+K
Sbjct: 253 LLNAIAKQPV--SVVVESKGRPFQLYKGGIFEGPCGTKVDHAVTAVGYGKSGGKGYILIK 312

[Close]

BLAST of CSPI03G10870 vs. TrEMBL
Match: I1Z744_JACME (Mexicain-like cystein protease (Fragment) OS=Jacaratia mexicana GN=CP5 PE=2 SV=1)

HSP 1 Score: 104.0 bits (258), Expect = 1.9e-19
Identity = 58/188 (30.85%), Postives = 91/188 (48.40%), Query Frame = 1


Query: 10  PKKVDWVESAVVTPPRDQGPTPTCWAYSGVAAIESMNKIKRGQLVNMTVVDVI-IDNIRF 69
           P+ +DW +   VTP ++Q P  +CWA+S VA +E +NKI  G+L++++  +++  D    
Sbjct: 110 PESIDWRQKGAVTPVKNQNPCGSCWAFSTVATVEGINKIVTGKLISLSEQELLDCDRRSH 169

Query: 70  WMDGAWPDVVFHDGVKQGIH------------KLKRKDSLLSKHPVC------------- 129
              G +           G+H            K + KD   SK  +              
Sbjct: 170 GCKGGYQTTSLQYVADNGVHTEKEYPYEKKQGKCRAKDKKGSKVKITGYKRVPANNEVSL 229

Query: 130 --------ISISVDEYGM-FRNYRGRLYYGPFGEIANHSMLAVGYSKDYSLLKNSWGCIW 163
                   +S+ V+  G  F+ Y+G ++ GP G   +H++ AVGY K+Y L+KNSWG  W
Sbjct: 230 IQAIANQPVSVVVESKGRAFQFYKGGIFEGPCGTKVDHAVTAVGYGKNYILIKNSWGPKW 289

[Close]

BLAST of CSPI03G10870 vs. TrEMBL
Match: R0H3G6_9BRAS (Uncharacterized protein OS=Capsella rubella GN=CARUB_v10006494mg PE=3 SV=1)

HSP 1 Score: 102.8 bits (255), Expect = 4.2e-19
Identity = 63/201 (31.34%), Postives = 105/201 (52.24%), Query Frame = 1


Query: 4   VAKDGLPKKVDWVESAVVTPPRDQGPTPTCWAYSGVAAIESMNKIKRGQLVNMT---VVD 63
           +A+D LP+ VDW +   V+  +DQG   +CWA+S VAA+ES+NKI  G+L++++   +VD
Sbjct: 126 LAEDQLPQSVDWRQKGAVSEIKDQGRCNSCWAFSTVAAVESINKIVTGELISLSEQELVD 185

Query: 64  VIIDNIRF---WMDGAWPDVVFHDGVK-------QGIHKLKRKDSLLSKHPVCI------ 123
             IDN       MD A+  ++ ++G++       Q +      +   SK  + I      
Sbjct: 186 CSIDNHGCNGGLMDSAFQFLINNNGLEYQSDYPYQAVQGYCNHNQNTSKKVIKIDGYEDV 245

Query: 124 -----------------SISVDEYGM-FRNYRGRLYYGPFGEIANHSMLAVGY----SKD 164
                            S+++D+    F  Y+  +Y GP G   +H+++ VGY     +D
Sbjct: 246 PANNENSLQKAVAHQPVSVAIDKKSQGFMLYKSGIYTGPCGTDLDHAVVIVGYGTENGQD 305

[Close]

BLAST of CSPI03G10870 vs. TAIR10
Match: AT4G23520.1 (AT4G23520.1 Cysteine proteinases superfamily protein)

HSP 1 Score: 95.9 bits (237), Expect = 2.6e-20
Identity = 61/202 (30.20%), Postives = 100/202 (49.50%), Query Frame = 1


Query: 4   VAKDGLPKKVDWVESAVVTPPRDQGPTPTCWAYSGVAAIESMNKIKRGQLVNMTVVDVII 63
           +A D LP+ VDW +   V+  +DQG   +CWA+S VAA+E +NKI  G+L++++  +++ 
Sbjct: 128 LAGDQLPESVDWRQEGAVSEIKDQGTCNSCWAFSTVAAVEGLNKIVTGELISLSEQELVD 187

Query: 64  DNI-------RFWMDGAWPDVVFHDGVK-------QGIH-KLKRKDSLLSKHPVC----- 123
            N+          MD A+  ++ ++G+        QG      RK S  +K         
Sbjct: 188 CNLVNNGCYGSGLMDTAFQFLINNNGLDSEKDYPYQGTQGSCNRKQSTSNKVITIDSYED 247

Query: 124 -----------------ISISVDEYGM-FRNYRGRLYYGPFGEIANHSMLAVGY----SK 164
                            +S+ VD+    F  YR  +Y GP G   +H+++ VGY     +
Sbjct: 248 VPANDEISLQKAVAHQPVSVGVDKKSQEFMLYRSCIYNGPCGTNLDHALVIVGYGSENGQ 307

[Close]

BLAST of CSPI03G10870 vs. TAIR10
Match: AT4G35350.1 (AT4G35350.1 xylem cysteine peptidase 1)

HSP 1 Score: 91.3 bits (225), Expect = 6.3e-19
Identity = 58/196 (29.59%), Postives = 94/196 (47.96%), Query Frame = 1


Query: 9   LPKKVDWVESAVVTPPRDQGPTPTCWAYSGVAAIESMNKIKRGQLVNMTVVDVIIDNIRF 68
           LPK VDW +   V P +DQG   +CWA+S VAA+E +N+I  G L +++  ++I  +  F
Sbjct: 137 LPKSVDWRKKGAVAPVKDQGQCGSCWAFSTVAAVEGINQITTGNLSSLSEQELIDCDTTF 196

Query: 69  -------WMDGAWPDVVFHDGV----------KQGIHKLKRKD----------------- 128
                   MD A+  ++   G+          ++GI + +++D                 
Sbjct: 197 NSGCNGGLMDYAFQYIISTGGLHKEDDYPYLMEEGICQEQKEDVERVTISGYEDVPENDD 256

Query: 129 ----SLLSKHPVCISISVDEYGMFRNYRGRLYYGPFGEIANHSMLAVGY----SKDYSLL 163
                 L+  PV ++I       F+ Y+G ++ G  G   +H + AVGY      DY ++
Sbjct: 257 ESLVKALAHQPVSVAIEASGRD-FQFYKGGVFNGKCGTDLDHGVAAVGYGSSKGSDYVIV 316

[Close]

BLAST of CSPI03G10870 vs. TAIR10
Match: AT1G29080.1 (AT1G29080.1 Papain family cysteine protease)

HSP 1 Score: 90.1 bits (222), Expect = 1.4e-18
Identity = 64/205 (31.22%), Postives = 91/205 (44.39%), Query Frame = 1


Query: 2   WN-VAKDGLPKKVDWVESAVVTPPRDQGPTPTCWAYSGVAAIESMNKIKRGQLVNMTVVD 61
           WN    D L    DW     VTP + QG    CWA+S +AA+E + KI RG L++++   
Sbjct: 122 WNWTVSDVLGTNKDWRNEGAVTPVKSQGECGGCWAFSAIAAVEGLTKIARGNLISLSEQQ 181

Query: 62  VIIDNIRFWMDG--------AWPDVVFHDG----------VKQGIHKLKRKDSLL----- 121
            ++D  R   +G        A+  ++ H G          VK+G  +   + ++L     
Sbjct: 182 -LLDCTREQNNGCKGGTFVNAFNYIIKHRGISSENEYPYQVKEGPCRSNARPAILIRGFE 241

Query: 122 --------------SKHPVCISISVDEYGMFRNYRGRLYYGP-FGEIANHSMLAVGYSKD 163
                         S+ PV ++I   E G F +Y G +Y     G   NH++  VGY   
Sbjct: 242 NVPSNNERALLEAVSRQPVAVAIDASEAG-FVHYSGGVYNARNCGTSVNHAVTLVGYGTS 301

[Close]

BLAST of CSPI03G10870 vs. TAIR10
Match: AT4G36880.1 (AT4G36880.1 cysteine proteinase1)

HSP 1 Score: 89.7 bits (221), Expect = 1.8e-18
Identity = 52/195 (26.67%), Postives = 96/195 (49.23%), Query Frame = 1


Query: 9   LPKKVDWVESAVVTPPRDQGPTPTCWAYSGVAAIESMNKIKRGQLVNMTVVDVIIDNIRF 68
           +P+ VDW +   V P +DQG   +CWA+S  AA+E +NKI  G+L++++  +++  +  +
Sbjct: 145 VPETVDWRQKGAVNPIKDQGTCGSCWAFSTTAAVEGINKIVTGELISLSEQELVDCDKSY 204

Query: 69  -------WMDGAWPDVVFHDGVK--------------------------QGIHKLKRKDS 128
                   MD A+  ++ + G+                            G   +  KD 
Sbjct: 205 NQGCNGGLMDYAFQFIMKNGGLNTEKDYPYRGFGGKCNSFLKNSRVVSIDGYEDVPTKDE 264

Query: 129 LLSKHPVC---ISISVDEYG-MFRNYRGRLYYGPFGEIANHSMLAVGYSK----DYSLLK 163
              K  +    +S++++  G +F++Y+  ++ G  G   +H+++AVGY      DY +++
Sbjct: 265 TALKKAISYQPVSVAIEAGGRIFQHYQSGIFTGSCGTNLDHAVVAVGYGSENGVDYWIVR 324

[Close]

BLAST of CSPI03G10870 vs. TAIR10
Match: AT3G19390.1 (AT3G19390.1 Granulin repeat cysteine protease family protein)

HSP 1 Score: 89.4 bits (220), Expect = 2.4e-18
Identity = 51/198 (25.76%), Postives = 94/198 (47.47%), Query Frame = 1


Query: 7   DGLPKKVDWVESAVVTPPRDQGPTPTCWAYSGVAAIESMNKIKRGQLVNMTVVDVIIDNI 66
           D LP  +DW     V P +DQG   +CWA+S + A+E +N+IK G+L++++  +++  + 
Sbjct: 127 DSLPDAIDWRAKGAVNPVKDQGSCGSCWAFSAIGAVEGINQIKTGELISLSEQELVDCDT 186

Query: 67  RF-------WMDGAWPDVVFHDGVK---------------------------QGIHKLKR 126
            +        MD A+  ++ + G+                             G   + +
Sbjct: 187 SYNDGCGGGLMDYAFKFIIENGGIDTEEDYPYIATDVNVCNSDKKNTRVVTIDGYEDVPQ 246

Query: 127 KDSLLSKHPVC---ISISVDEYG-MFRNYRGRLYYGPFGEIANHSMLAVGY----SKDYS 163
            D    K  +    IS++++  G  F+ Y   ++ G  G   +H ++AVGY     +DY 
Sbjct: 247 NDEKSLKKALANQPISVAIEAGGRAFQLYTSGVFTGTCGTSLDHGVVAVGYGSEGGQDYW 306

[Close]

BLAST of CSPI03G10870 vs. NCBI nr
Match: gi|778688435|ref|XP_011652749.1| (PREDICTED: mexicain [Cucumis sativus])

HSP 1 Score: 164.9 bits (416), Expect = 1.3e-37
Identity = 73/76 (96.05%), Postives = 74/76 (97.37%), Query Frame = 1


Query: 95  SLLSKHPVCISISVDEYGMFRNYRGRLYYGPFGEIANHSMLAVGYSKDYSLLKNSWGCIW 154
           SLLSKHPVCISISVDEYGM+RNYRGRLYYGP GEIANHSMLAVGYSKDY LLKNSWGCIW
Sbjct: 22  SLLSKHPVCISISVDEYGMYRNYRGRLYYGPLGEIANHSMLAVGYSKDYLLLKNSWGCIW 81

Query: 155 GENGYCRIATQQVFRK 171
           GENGYCRIATQQVFRK
Sbjct: 82  GENGYCRIATQQVFRK 97

[Close]

BLAST of CSPI03G10870 vs. NCBI nr
Match: gi|700201604|gb|KGN56737.1| (hypothetical protein Csa_3G131900 [Cucumis sativus])

HSP 1 Score: 162.2 bits (409), Expect = 8.3e-37
Identity = 76/91 (83.52%), Postives = 80/91 (87.91%), Query Frame = 1


Query: 1  MWNV-------AKDGLPKKVDWVESAVVTPPRDQGPTPTCWAYSGVAAIESMNKIKRGQL 60
          MW +        +DGLPKKVDWVESA VTPPRDQGP+PTC AYSGVAAIESMNKIKRGQL
Sbjct: 1  MWRIKHPHIFQTRDGLPKKVDWVESAAVTPPRDQGPSPTCRAYSGVAAIESMNKIKRGQL 60

Query: 61 VNMTVVDVIIDNIRFWMDGAWPDVVFHDGVK 85
          VN+TVVDVIIDNIRFWMDGAWPDVVFHDGVK
Sbjct: 61 VNLTVVDVIIDNIRFWMDGAWPDVVFHDGVK 91

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BLAST of CSPI03G10870 vs. NCBI nr
Match: gi|778688440|ref|XP_011652750.1| (PREDICTED: ananain-like [Cucumis sativus])

HSP 1 Score: 160.6 bits (405), Expect = 2.4e-36
Identity = 74/78 (94.87%), Postives = 76/78 (97.44%), Query Frame = 1


Query: 7   DGLPKKVDWVESAVVTPPRDQGPTPTCWAYSGVAAIESMNKIKRGQLVNMTVVDVIIDNI 66
           DGLPKKVDWVESA VTPPRDQGP+PTC AYSGVAAIESMNKIKRGQLVN+TVVDVIIDNI
Sbjct: 172 DGLPKKVDWVESAAVTPPRDQGPSPTCRAYSGVAAIESMNKIKRGQLVNLTVVDVIIDNI 231

Query: 67  RFWMDGAWPDVVFHDGVK 85
           RFWMDGAWPDVVFHDGVK
Sbjct: 232 RFWMDGAWPDVVFHDGVK 249

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BLAST of CSPI03G10870 vs. NCBI nr
Match: gi|312100382|gb|ADQ27799.1| (mitogenic proteinase [Vasconcellea cundinamarcensis])

HSP 1 Score: 108.2 bits (269), Expect = 1.4e-20
Identity = 59/189 (31.22%), Postives = 90/189 (47.62%), Query Frame = 1


Query: 10  PKKVDWVESAVVTPPRDQGPTPTCWAYSGVAAIESMNKIKRGQLVNMTVVDVI-IDNIRF 69
           P+ +DW +   VTP +DQ P  +CWA+S VA +E +NKI  G+L++++  +++  D    
Sbjct: 2   PESIDWRQKGAVTPVKDQNPCGSCWAFSTVATVEGINKIVTGKLISLSEQELLDCDRRSH 61

Query: 70  WMDGAWPDVVFHDGVKQGIH------------KLKRKDS--------------------- 129
             +G +        V  G+H              + KD                      
Sbjct: 62  GCNGGYQTTSLQYVVDNGVHTEYEYPYEKKQGNCRAKDKKGLKVQITGYKRVPPNDEISL 121

Query: 130 --LLSKHPVCISISVDEYGMFRNYRGRLYYGPFGEIANHSMLAVGYSKDYSLLKNSWGCI 163
             +++  PV + I   +   F  YRG +Y GP G   +H++ A+GY KDY L+KNSWG  
Sbjct: 122 IKVIANQPVSVLIESKDRS-FHFYRGGIYKGPCGTRLDHAVTAIGYGKDYILIKNSWGPN 181

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BLAST of CSPI03G10870 vs. NCBI nr
Match: gi|116666824|pdb|2BDZ|A (Chain A, Mexicain From Jacaratia Mexicana)

HSP 1 Score: 107.1 bits (266), Expect = 3.2e-20
Identity = 63/190 (33.16%), Postives = 91/190 (47.89%), Query Frame = 1


Query: 10  PKKVDWVESAVVTPPRDQGPTPTCWAYSGVAAIESMNKIKRGQLVNMTVVDVI-IDNIRF 69
           P+ +DW E   VTP ++Q P  +CWA+S VA IE +NKI  GQL++++  +++  +    
Sbjct: 2   PESIDWREKGAVTPVKNQNPCGSCWAFSTVATIEGINKIITGQLISLSEQELLDCERRSH 61

Query: 70  WMDGAWPDVVFHDGVKQGIH------------KLKRKDS--------------------- 129
             DG +        V  G+H            + + KD                      
Sbjct: 62  GCDGGYQTTSLQYVVDNGVHTEREYPYEKKQGRCRAKDKKGPKVYITGYKYVPANDEISL 121

Query: 130 --LLSKHPVCISISVDEYGM-FRNYRGRLYYGPFGEIANHSMLAVGYSKDYSLLKNSWGC 163
              ++  PV  S+  D  G  F+ Y+G +Y GP G   +H++ AVGY K Y LLKNSWG 
Sbjct: 122 IQAIANQPV--SVVTDSRGRGFQFYKGGIYEGPCGTNTDHAVTAVGYGKTYLLLKNSWGP 181

The following BLAST results are available for this feature:

BLAST of CSPI03G10870 vs. Swiss-Prot


Match Name	E-value	Identity	Description
PAPA3_CARPA	1.3e-21	32.82	Caricain OS=Carica papaya PE=1 SV=2	[more]
ANAN_ANACO	2.4e-20	26.42	Ananain OS=Ananas comosus GN=AN1 PE=1 SV=2	[more]
RDL6_ARATH	4.6e-19	30.20	Probable cysteine protease RDL6 OS=Arabidopsis thaliana GN=RDL6 PE=3 SV=1	[more]
MDO1_PSEMR	7.8e-19	27.51	Macrodontain-1 OS=Pseudananas macrodontes PE=1 SV=1	[more]
BROM1_ANACO	1.7e-18	26.15	Fruit bromelain OS=Ananas comosus PE=1 SV=1	[more]

BLAST of CSPI03G10870 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L7Y4_CUCSA	5.8e-37	83.52	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G131900 PE=4 SV=1	[more]
E5LBE8_VASCU	9.9e-21	31.22	Mitogenic proteinase (Fragment) OS=Vasconcellea cundinamarcensis PE=2 SV=1	[more]
Q42673_CARPA	1.4e-19	32.82	Papaya proteinase omega OS=Carica papaya GN=omegaii PE=2 SV=1	[more]
I1Z744_JACME	1.9e-19	30.85	Mexicain-like cystein protease (Fragment) OS=Jacaratia mexicana GN=CP5 PE=2 SV=1	[more]
R0H3G6_9BRAS	4.2e-19	31.34	Uncharacterized protein OS=Capsella rubella GN=CARUB_v10006494mg PE=3 SV=1	[more]

BLAST of CSPI03G10870 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G23520.1	2.6e-20	30.20	Cysteine proteinases superfamily protein	[more]
AT4G35350.1	6.3e-19	29.59	xylem cysteine peptidase 1	[more]
AT1G29080.1	1.4e-18	31.22	Papain family cysteine protease	[more]
AT4G36880.1	1.8e-18	26.67	cysteine proteinase1	[more]
AT3G19390.1	2.4e-18	25.76	Granulin repeat cysteine protease family protein	[more]

BLAST of CSPI03G10870 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778688435\|ref\|XP_011652749.1\|	1.3e-37	96.05	PREDICTED: mexicain [Cucumis sativus]	[more]
gi\|700201604\|gb\|KGN56737.1\|	8.3e-37	83.52	hypothetical protein Csa_3G131900 [Cucumis sativus]	[more]
gi\|778688440\|ref\|XP_011652750.1\|	2.4e-36	94.87	PREDICTED: ananain-like [Cucumis sativus]	[more]
gi\|312100382\|gb\|ADQ27799.1\|	1.4e-20	31.22	mitogenic proteinase [Vasconcellea cundinamarcensis]	[more]
gi\|116666824\|pdb\|2BDZ\|A	3.2e-20	33.16	Chain A, Mexicain From Jacaratia Mexicana	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR000668	Peptidase_C1A_C
IPR013128	Peptidase_C1A
IPR025660	Pept_his_AS

Vocabulary: **Biological Process**
Term	Definition
GO:0006508	proteolysis

Vocabulary: **Molecular Function**
Term	Definition
GO:0008234	cysteine-type peptidase activity

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0008234	cysteine-type peptidase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G10870.1	CSPI03G10870.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000668	Peptidase C1A, papain C-terminal	PFAM	PF00112	Peptidase_C1	coord: 9..62 score: 2.5E-8coord: 91..165 score: 3.0
IPR000668	Peptidase C1A, papain C-terminal	SMART	SM00645	pept_c1	coord: 9..170 score: 9.
IPR013128	Peptidase C1A	PANTHER	PTHR12411	CYSTEINE PROTEASE FAMILY C1-RELATED	coord: 9..163 score: 7.7
IPR025660	Cysteine peptidase, histidine active site	PROSITE	PS00639	THIOL_PROTEASE_HIS	coord: 130..140 scor
None	No IPR available	GENE3D	G3DSA:3.90.70.10		coord: 7..87 score: 1.0E-13coord: 93..166 score: 3.3
None	No IPR available	PANTHER	PTHR12411:SF364	SUBFAMILY NOT NAMED	coord: 9..163 score: 7.7
None	No IPR available	unknown	SSF54001	Cysteine proteinases	coord: 6..164 score: 4.56

Synteny

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CSPI03G10870	Silver-seed gourd	carcpiB0200
CSPI03G10870	Silver-seed gourd	carcpiB0261
CSPI03G10870	Silver-seed gourd	carcpiB0814
CSPI03G10870	Silver-seed gourd	carcpiB0998
CSPI03G10870	Silver-seed gourd	carcpiB1115
CSPI03G10870	Cucumber (Chinese Long) v3	cpicucB144
CSPI03G10870	Cucumber (Chinese Long) v3	cpicucB156
CSPI03G10870	Watermelon (97103) v2	cpiwmbB209
CSPI03G10870	Watermelon (97103) v2	cpiwmbB239
CSPI03G10870	Watermelon (97103) v2	cpiwmbB250
CSPI03G10870	Wax gourd	cpiwgoB251
CSPI03G10870	Wax gourd	cpiwgoB270
CSPI03G10870	Wax gourd	cpiwgoB306
CSPI03G10870	Wild cucumber (PI 183967)	cpicpiB063
CSPI03G10870	Wild cucumber (PI 183967)	cpicpiB071
CSPI03G10870	Wild cucumber (PI 183967)	cpicpiB104
CSPI03G10870	Cucumber (Gy14) v1	cgycpiB383
CSPI03G10870	Cucurbita maxima (Rimu)	cmacpiB255
CSPI03G10870	Cucurbita maxima (Rimu)	cmacpiB826
CSPI03G10870	Cucurbita maxima (Rimu)	cmacpiB845
CSPI03G10870	Cucurbita moschata (Rifu)	cmocpiB243
CSPI03G10870	Cucurbita moschata (Rifu)	cmocpiB353
CSPI03G10870	Cucurbita moschata (Rifu)	cmocpiB588
CSPI03G10870	Cucurbita moschata (Rifu)	cmocpiB814
CSPI03G10870	Cucurbita moschata (Rifu)	cmocpiB883
CSPI03G10870	Cucumber (Chinese Long) v2	cpicuB120
CSPI03G10870	Melon (DHL92) v3.5.1	cpimeB226
CSPI03G10870	Melon (DHL92) v3.5.1	cpimeB230
CSPI03G10870	Watermelon (Charleston Gray)	cpiwcgB226
CSPI03G10870	Watermelon (Charleston Gray)	cpiwcgB255
CSPI03G10870	Watermelon (Charleston Gray)	cpiwcgB264
CSPI03G10870	Watermelon (97103) v1	cpiwmB221
CSPI03G10870	Watermelon (97103) v1	cpiwmB231
CSPI03G10870	Cucurbita pepo (Zucchini)	cpecpiB611
CSPI03G10870	Cucurbita pepo (Zucchini)	cpecpiB861
CSPI03G10870	Bottle gourd (USVL1VR-Ls)	cpilsiB149
CSPI03G10870	Bottle gourd (USVL1VR-Ls)	cpilsiB207
CSPI03G10870	Melon (DHL92) v3.6.1	cpimedB224
CSPI03G10870	Cucumber (Gy14) v2	cgybcpiB073
CSPI03G10870	Cucumber (Gy14) v2	cgybcpiB110
CSPI03G10870	Cucumber (Gy14) v2	cgybcpiB122