CSPI03G04270 (gene) Wild cucumber (PI 183967)

Overview
NameCSPI03G04270
Typegene
OrganismCucumis sativus (Wild cucumber (PI 183967))
DescriptionNuclear transcription factor Y protein
LocationChr3 : 3491286 .. 3492005 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGGAAGGAAATGAATATGGATATTTTTATGTATATGTTATCTTGATGAATCTGAATTTGAAGTACTTGTGTCGTTATTCAGGACTGAACACGATGCTGACCCCAAACAACGCAACCTCCATCAACAGCAATCCAAACACCAATCACCATCTCTCGAACGGAGGAACCTCCATTGGCAACTCCTCAGCTACCAGCCCCCTCTCGGTCGCCGCCACTGCCAACAATAATGAGCAAAACCAACAATGCATGGTGCGAGAACAAGACCAATACATGCCGATGGCTAATGTGATACGAATCATGAAGCGGATTTTACCCTCCCATGCAAAAATATCCGACGACACCAAAGAGACCATCCAAGAATGTGTGTCGGAGTACATTAGCTTCATCACTAGCGAGGCGAACGAACGATGCCACCGGGAGCAACGCAAGACGGTGACTGCAGAGGATGTTCTTTGGGCTATGGGGAAGTTAGGTTTTGACAACTACATAGAACCGCTAACTGTTTTCCTTAACCGCTATCGTGAGTCGGAGAGTATTCGTACTGAACCAATGTTGAGGCGCAATGTGGGTTATGGGGCACAAGTGGGGATGATATCACCGTATGGGCAGGCGTTTCAGATAGGGCAAGTCCCTGCAGGGATGTTTGATGCAATGGGTGGATACTATGGTGGAGGAGGGAGCGGTGGGCCGAGCGCAGGGAACAACAGCCAGCTTTGA

mRNA sequence

ATGAAGGAAGGAAATGAATATGGATATTTTTATGTATATGTTATCTTGATGAATCTGAATTTGAAGTACTTGTGTCGTTATTCAGGACTGAACACGATGCTGACCCCAAACAACGCAACCTCCATCAACAGCAATCCAAACACCAATCACCATCTCTCGAACGGAGGAACCTCCATTGGCAACTCCTCAGCTACCAGCCCCCTCTCGGTCGCCGCCACTGCCAACAATAATGAGCAAAACCAACAATGCATGGTGCGAGAACAAGACCAATACATGCCGATGGCTAATGTGATACGAATCATGAAGCGGATTTTACCCTCCCATGCAAAAATATCCGACGACACCAAAGAGACCATCCAAGAATGTGTGTCGGAGTACATTAGCTTCATCACTAGCGAGGCGAACGAACGATGCCACCGGGAGCAACGCAAGACGGTGACTGCAGAGGATGTTCTTTGGGCTATGGGGAAGTTAGGTTTTGACAACTACATAGAACCGCTAACTGTTTTCCTTAACCGCTATCGTGAGTCGGAGAGTATTCGTACTGAACCAATGTTGAGGCGCAATGTGGGTTATGGGGCACAAGTGGGGATGATATCACCGTATGGGCAGGCGTTTCAGATAGGGCAAGTCCCTGCAGGGATGTTTGATGCAATGGGTGGATACTATGGTGGAGGAGGGAGCGGTGGGCCGAGCGCAGGGAACAACAGCCAGCTTTGA

Coding sequence (CDS)

ATGAAGGAAGGAAATGAATATGGATATTTTTATGTATATGTTATCTTGATGAATCTGAATTTGAAGTACTTGTGTCGTTATTCAGGACTGAACACGATGCTGACCCCAAACAACGCAACCTCCATCAACAGCAATCCAAACACCAATCACCATCTCTCGAACGGAGGAACCTCCATTGGCAACTCCTCAGCTACCAGCCCCCTCTCGGTCGCCGCCACTGCCAACAATAATGAGCAAAACCAACAATGCATGGTGCGAGAACAAGACCAATACATGCCGATGGCTAATGTGATACGAATCATGAAGCGGATTTTACCCTCCCATGCAAAAATATCCGACGACACCAAAGAGACCATCCAAGAATGTGTGTCGGAGTACATTAGCTTCATCACTAGCGAGGCGAACGAACGATGCCACCGGGAGCAACGCAAGACGGTGACTGCAGAGGATGTTCTTTGGGCTATGGGGAAGTTAGGTTTTGACAACTACATAGAACCGCTAACTGTTTTCCTTAACCGCTATCGTGAGTCGGAGAGTATTCGTACTGAACCAATGTTGAGGCGCAATGTGGGTTATGGGGCACAAGTGGGGATGATATCACCGTATGGGCAGGCGTTTCAGATAGGGCAAGTCCCTGCAGGGATGTTTGATGCAATGGGTGGATACTATGGTGGAGGAGGGAGCGGTGGGCCGAGCGCAGGGAACAACAGCCAGCTTTGA
Homology
[Close]
BLAST of CSPI03G04270 vs. Swiss-Prot
Match: NFYB9_ARATH (Nuclear transcription factor Y subunit B-9 OS=Arabidopsis thaliana GN=NFYB9 PE=1 SV=2)

HSP 1 Score: 183.0 bits (463), Expect = 4.0e-45
Identity = 104/180 (57.78%), Postives = 120/180 (66.67%), Query Frame = 1 

Query: 65  TSPLSVAATANNN-----EQNQQCMVREQDQYMPMANVIRIMKRILPSHAKISDDTKETI 124
           TS + VA   + N     +Q   C+ REQDQYMP+ANVIRIM++ LPSHAKISDD KETI
Sbjct: 32  TSSVVVAGAGDKNNGIVVQQQPPCVAREQDQYMPIANVIRIMRKTLPSHAKISDDAKETI 91

Query: 125 QECVSEYISFITSEANERCHREQRKTVTAEDVLWAMGKLGFDNYIEPLTVFLNRYRESE- 184
           QECVSEYISF+T EANERC REQRKT+TAED+LWAM KLGFDNY++PLTVF+NRYRE E 
Sbjct: 92  QECVSEYISFVTGEANERCQREQRKTITAEDILWAMSKLGFDNYVDPLTVFINRYREIET 151

Query: 185 ----SIRTEPMLRRNVGYGAQVGMISPYGQAFQIGQVPAGMFD---AMGG--YYGGGGSG 230
               ++R EP   R    G  +G   P       G    GM D    MGG  YY  G SG
Sbjct: 152 DRGSALRGEPPSLRQTYGGNGIGFHGPSHGLPPPGPYGYGMLDQSMVMGGGRYYQNGSSG 211

[Close]
BLAST of CSPI03G04270 vs. Swiss-Prot
Match: NFYB6_ARATH (Nuclear transcription factor Y subunit B-6 OS=Arabidopsis thaliana GN=NFYB6 PE=1 SV=2)

HSP 1 Score: 174.1 bits (440), Expect = 1.8e-42
Identity = 84/109 (77.06%), Postives = 98/109 (89.91%), Query Frame = 1 

Query: 73  TANNNEQNQQCMVREQDQYMPMANVIRIMKRILPSHAKISDDTKETIQECVSEYISFITS 132
           T+N  E+  +C VREQD++MP+ANVIRIM+RILP+HAKISDD+KETIQECVSEYISFIT 
Sbjct: 46  TSNGGEE--ECTVREQDRFMPIANVIRIMRRILPAHAKISDDSKETIQECVSEYISFITG 105

Query: 133 EANERCHREQRKTVTAEDVLWAMGKLGFDNYIEPLTVFLNRYRESESIR 182
           EANERC REQRKT+TAEDVLWAM KLGFD+YIEPLT++L+RYRE E  R
Sbjct: 106 EANERCQREQRKTITAEDVLWAMSKLGFDDYIEPLTLYLHRYRELEGER 152

[Close]
BLAST of CSPI03G04270 vs. Swiss-Prot
Match: NFYB2_ARATH (Nuclear transcription factor Y subunit B-2 OS=Arabidopsis thaliana GN=NFYB2 PE=2 SV=1)

HSP 1 Score: 138.3 bits (347), Expect = 1.1e-31
Identity = 79/159 (49.69%), Postives = 106/159 (66.67%), Query Frame = 1 

Query: 75  NNNEQNQQCMV--REQDQYMPMANVIRIMKRILPSHAKISDDTKETIQECVSEYISFITS 134
           N N QN Q  +  REQD+++P+ANV RIMK+ LP++AKIS D KET+QECVSE+ISF+T 
Sbjct: 13  NGNNQNGQSSLSPREQDRFLPIANVSRIMKKALPANAKISKDAKETMQECVSEFISFVTG 72

Query: 135 EANERCHREQRKTVTAEDVLWAMGKLGFDNYIEPLTVFLNRYRESESIRTEPMLRRNVGY 194
           EA+++C +E+RKT+  +D+LWAM  LGF++Y+EPL V+L R+RE E  RT        G 
Sbjct: 73  EASDKCQKEKRKTINGDDLLWAMTTLGFEDYVEPLKVYLQRFREIEGERT--------GL 132

Query: 195 G-AQVGMISPYGQAFQIGQVPAGMFDAM---GGYYGGGG 228
           G  Q G           G+V     DA+   GG+YGGGG
Sbjct: 133 GRPQTG-----------GEVGEHQRDAVGDGGGFYGGGG 152

[Close]
BLAST of CSPI03G04270 vs. Swiss-Prot
Match: NFYB3_ARATH (Nuclear transcription factor Y subunit B-3 OS=Arabidopsis thaliana GN=NFYB3 PE=2 SV=1)

HSP 1 Score: 136.7 bits (343), Expect = 3.3e-31
Identity = 72/137 (52.55%), Postives = 95/137 (69.34%), Query Frame = 1 

Query: 86  REQDQYMPMANVIRIMKRILPSHAKISDDTKETIQECVSEYISFITSEANERCHREQRKT 145
           REQD+++P+ANV RIMK+ LP++AKIS D KET+QECVSE+ISFIT EA+++C RE+RKT
Sbjct: 20  REQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVSEFISFITGEASDKCQREKRKT 79

Query: 146 VTAEDVLWAMGKLGFDNYIEPLTVFLNRYRESESIRTEPMLRR--NVGYGAQVGMISPYG 205
           +  +D+LWAM  LGF++Y+EPL V+L +YRE E  +T    R+    G G   G  S  G
Sbjct: 80  INGDDLLWAMTTLGFEDYVEPLKVYLQKYREVEGEKTTTAGRQGDKEGGGGGGGAGSGSG 139

Query: 206 QAFQIGQVPAGMFDAMG 221
            A   G    GM   MG
Sbjct: 140 GAPMYG---GGMVTTMG 153

[Close]
BLAST of CSPI03G04270 vs. Swiss-Prot
Match: NFYB3_ORYSJ (Nuclear transcription factor Y subunit B-3 OS=Oryza sativa subsp. japonica GN=NFYB3 PE=1 SV=2)

HSP 1 Score: 135.2 bits (339), Expect = 9.5e-31
Identity = 58/94 (61.70%), Postives = 81/94 (86.17%), Query Frame = 1 

Query: 85  VREQDQYMPMANVIRIMKRILPSHAKISDDTKETIQECVSEYISFITSEANERCHREQRK 144
           VREQD+++P+AN+ RIMK+ +P++ KI+ D KET+QECVSE+ISFITSEA+++C RE+RK
Sbjct: 36  VREQDRFLPIANISRIMKKAIPANGKIAKDAKETVQECVSEFISFITSEASDKCQREKRK 95

Query: 145 TVTAEDVLWAMGKLGFDNYIEPLTVFLNRYRESE 179
           T+  +D+LWAM  LGF++YIEPL V+L +YRE E
Sbjct: 96  TINGDDLLWAMATLGFEDYIEPLKVYLQKYREME 129

[Close]
BLAST of CSPI03G04270 vs. TrEMBL
Match: A0A0A0L2U1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G049440 PE=4 SV=1)

HSP 1 Score: 457.2 bits (1175), Expect = 1.2e-125
Identity = 233/239 (97.49%), Postives = 235/239 (98.33%), Query Frame = 1 

Query: 1   MKEGNEYGYFYVYVILMNLNLKYLCRYSGLNTMLTPNNATSINSNPNTNHHLSNGGTSIG 60
           MKEGNEYGYFYVYVILMNLNLKYLCRYSGLNTMLTPNN TSINSNPNTN HLSNGGTSIG
Sbjct: 1   MKEGNEYGYFYVYVILMNLNLKYLCRYSGLNTMLTPNNPTSINSNPNTNQHLSNGGTSIG 60

Query: 61  NSSATSPLSVAATANNNEQNQQCMVREQDQYMPMANVIRIMKRILPSHAKISDDTKETIQ 120
           NSSATSPLSVAATANNNEQNQQC+VREQDQYMPMANVIRIMKRILPSHAKISDDTKETIQ
Sbjct: 61  NSSATSPLSVAATANNNEQNQQCLVREQDQYMPMANVIRIMKRILPSHAKISDDTKETIQ 120

Query: 121 ECVSEYISFITSEANERCHREQRKTVTAEDVLWAMGKLGFDNYIEPLTVFLNRYRESESI 180
           ECVSEYISFITSEANERC REQRKTVTAEDVLWAMGKLGFD+YIEPLTVFLNRYRESESI
Sbjct: 121 ECVSEYISFITSEANERCQREQRKTVTAEDVLWAMGKLGFDDYIEPLTVFLNRYRESESI 180

Query: 181 RTEPMLRRNVGYGAQVGMISPYGQAFQIGQVPAGMFDAMGGYYGGGGSGGPSAGNNSQL 240
           RTEPMLRRNV YGAQVGMISPYGQAFQIGQVPAGMFDAMGGYYGGGGSGGPSAGNNSQL
Sbjct: 181 RTEPMLRRNVDYGAQVGMISPYGQAFQIGQVPAGMFDAMGGYYGGGGSGGPSAGNNSQL 239

[Close]
BLAST of CSPI03G04270 vs. TrEMBL
Match: A0A0A0L4W1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G055940 PE=4 SV=1)

HSP 1 Score: 369.0 bits (946), Expect = 4.4e-99
Identity = 194/239 (81.17%), Postives = 200/239 (83.68%), Query Frame = 1 

Query: 1   MKEGNEYGYFYVYVILMNLNLKYLCRYSGLNTMLTPNNATSINSNPNTNHHLSNGGTSIG 60
           +KEGNEYGYFYVYV+LMNLNL+YLCRYS LN MLTPNN  SIN+NPNTNHHLS+GGTSIG
Sbjct: 12  IKEGNEYGYFYVYVLLMNLNLQYLCRYSELNMMLTPNNPNSINNNPNTNHHLSDGGTSIG 71

Query: 61  NSSATSPLSVAATANNNEQNQQCMVREQDQYMPMANVIRIMKRILPSHAKISDDTKETIQ 120
           NSSATSPLSVAATANNNEQNQQC+VREQDQYMPMAN                        
Sbjct: 72  NSSATSPLSVAATANNNEQNQQCLVREQDQYMPMAN------------------------ 131

Query: 121 ECVSEYISFITSEANERCHREQRKTVTAEDVLWAMGKLGFDNYIEPLTVFLNRYRESESI 180
                YISFITSEANERC REQRKTVTAEDVLWAMGKLGFDNYIEPLTVFLNRYRESESI
Sbjct: 132 -----YISFITSEANERCQREQRKTVTAEDVLWAMGKLGFDNYIEPLTVFLNRYRESESI 191

Query: 181 RTEPMLRRNVGYGAQVGMISPYGQAFQIGQVPAGMFDAMGGYYGGGGSGGPSAGNNSQL 240
           RTEPMLRRNV YG QVGMISPYGQAFQIG VPAGMFDAMGGYYGGGGS GPS GNNSQL
Sbjct: 192 RTEPMLRRNVDYGPQVGMISPYGQAFQIGHVPAGMFDAMGGYYGGGGSCGPSTGNNSQL 221

[Close]
BLAST of CSPI03G04270 vs. TrEMBL
Match: A0A0B0PGM2_GOSAR (Nuclear transcription factor Y subunit B-9-like protein OS=Gossypium arboreum GN=F383_03265 PE=4 SV=1)

HSP 1 Score: 228.4 bits (581), Expect = 9.2e-57
Identity = 137/243 (56.38%), Postives = 165/243 (67.90%), Query Frame = 1 

Query: 1   MKEGNEYGYFYVYVILMNLNLKYLCRYSGLNTMLTPNNATSINSNPNTNHHLSNGGTSIG 60
           M+ G+ +  FY          K+    SGL+ M    +  S NSN NTN  ++N   + G
Sbjct: 1   MERGDGFNRFY----------KHAKSSSGLSIM----HGDSSNSNTNTNTIINNNAANNG 60

Query: 61  NSSATSPLSVAATANNNEQNQQCMVREQDQYMPMANVIRIMKRILPSHAKISDDTKETIQ 120
           N+           AN+N     CMVREQDQYMP+ANVIRIM+RILP+HAKISDD KETIQ
Sbjct: 61  NA-----------ANSNTMPPPCMVREQDQYMPIANVIRIMRRILPTHAKISDDAKETIQ 120

Query: 121 ECVSEYISFITSEANERCHREQRKTVTAEDVLWAMGKLGFDNYIEPLTVFLNRYRESE-- 180
           ECVSEYISFIT EANERC REQRKT+TAEDVLWAMGKLGFD+Y+EPLTVFLNRYRE+E  
Sbjct: 121 ECVSEYISFITGEANERCQREQRKTITAEDVLWAMGKLGFDDYVEPLTVFLNRYRENENE 180

Query: 181 --SIRTEPMLRRNVGYGAQVGMISPYGQAFQIGQVPAGMFD---AMGGYY---GGGGSGG 234
             S+R+EPML+R + YG  + M++PYG  F +G    G+FD   AMGGY      GG GG
Sbjct: 181 RTSLRSEPMLKRGIDYGPSM-MMAPYGAGFHVGH-QQGIFDGATAMGGYMRDGSSGGGGG 216

[Close]
BLAST of CSPI03G04270 vs. TrEMBL
Match: A0A0D2SEP9_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_013G152500 PE=4 SV=1)

HSP 1 Score: 221.9 bits (564), Expect = 8.6e-55
Identity = 125/198 (63.13%), Postives = 148/198 (74.75%), Query Frame = 1 

Query: 50  HHLSNGGTSI----GNSSATSPLSVAATANNNEQNQQCMVREQDQYMPMANVIRIMKRIL 109
           H  S+ G SI     ++S T+ +     AN+N     CMVREQDQYMP+ANVIRIM+RIL
Sbjct: 13  HAKSSSGLSIMHGDSSNSNTNTIINNNAANSNTMPPPCMVREQDQYMPIANVIRIMRRIL 72

Query: 110 PSHAKISDDTKETIQECVSEYISFITSEANERCHREQRKTVTAEDVLWAMGKLGFDNYIE 169
           P+HAKISDD KETIQECVSEYISFIT EANERC REQRKT+TAEDVLWAMGKLGFD+Y+E
Sbjct: 73  PTHAKISDDAKETIQECVSEYISFITGEANERCQREQRKTITAEDVLWAMGKLGFDDYVE 132

Query: 170 PLTVFLNRYRESE----SIRTEPMLRRNVGYGAQVGMISPYGQAFQIGQVPAGMFD---A 229
           PLTVFLNRYRE+E    S+R+EPML+R + YG  + M++PYG  F +G    G+FD   A
Sbjct: 133 PLTVFLNRYRENENERTSLRSEPMLKRGIDYGPSM-MMAPYGAGFHVGH-QQGIFDGATA 192

Query: 230 MGGYY---GGGGSGGPSA 234
           MGGY      GG GGPS+
Sbjct: 193 MGGYMRDGSSGGGGGPSS 208

[Close]
BLAST of CSPI03G04270 vs. TrEMBL
Match: A0A0A0L645_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G048940 PE=4 SV=1)

HSP 1 Score: 221.9 bits (564), Expect = 8.6e-55
Identity = 113/126 (89.68%), Postives = 118/126 (93.65%), Query Frame = 1 

Query: 33  MLTPNNATSINSNPNTNHHLSNGGTSIGNSSATSPLSVAATANNNEQNQQCMVREQDQYM 92
           MLTPN  TSIN+ PNTNHHLSNGGT+ GNSSATSPLSVAATANNNEQNQQC+VREQDQYM
Sbjct: 2   MLTPNKPTSINNTPNTNHHLSNGGTATGNSSATSPLSVAATANNNEQNQQCVVREQDQYM 61

Query: 93  PMANVIRIMKRILPSHAKISDDTKETIQECVSEYISFITSEANERCHREQRKTVTAEDVL 152
           P+ANVIR M+RILPSHAKISDD KETIQECVSEYISFIT EANERC REQRKTVTAEDVL
Sbjct: 62  PIANVIRSMRRILPSHAKISDDAKETIQECVSEYISFITGEANERCQREQRKTVTAEDVL 121

Query: 153 WAMGKL 159
           WAMGKL
Sbjct: 122 WAMGKL 127

[Close]
BLAST of CSPI03G04270 vs. TAIR10
Match: AT1G21970.1 (AT1G21970.1 Histone superfamily protein)

HSP 1 Score: 183.0 bits (463), Expect = 2.2e-46
Identity = 104/180 (57.78%), Postives = 120/180 (66.67%), Query Frame = 1 

Query: 65  TSPLSVAATANNN-----EQNQQCMVREQDQYMPMANVIRIMKRILPSHAKISDDTKETI 124
           TS + VA   + N     +Q   C+ REQDQYMP+ANVIRIM++ LPSHAKISDD KETI
Sbjct: 32  TSSVVVAGAGDKNNGIVVQQQPPCVAREQDQYMPIANVIRIMRKTLPSHAKISDDAKETI 91

Query: 125 QECVSEYISFITSEANERCHREQRKTVTAEDVLWAMGKLGFDNYIEPLTVFLNRYRESE- 184
           QECVSEYISF+T EANERC REQRKT+TAED+LWAM KLGFDNY++PLTVF+NRYRE E 
Sbjct: 92  QECVSEYISFVTGEANERCQREQRKTITAEDILWAMSKLGFDNYVDPLTVFINRYREIET 151

Query: 185 ----SIRTEPMLRRNVGYGAQVGMISPYGQAFQIGQVPAGMFD---AMGG--YYGGGGSG 230
               ++R EP   R    G  +G   P       G    GM D    MGG  YY  G SG
Sbjct: 152 DRGSALRGEPPSLRQTYGGNGIGFHGPSHGLPPPGPYGYGMLDQSMVMGGGRYYQNGSSG 211

[Close]
BLAST of CSPI03G04270 vs. TAIR10
Match: AT5G47670.1 (AT5G47670.1 nuclear factor Y, subunit B6)

HSP 1 Score: 174.1 bits (440), Expect = 1.0e-43
Identity = 84/109 (77.06%), Postives = 98/109 (89.91%), Query Frame = 1 

Query: 73  TANNNEQNQQCMVREQDQYMPMANVIRIMKRILPSHAKISDDTKETIQECVSEYISFITS 132
           T+N  E+  +C VREQD++MP+ANVIRIM+RILP+HAKISDD+KETIQECVSEYISFIT 
Sbjct: 46  TSNGGEE--ECTVREQDRFMPIANVIRIMRRILPAHAKISDDSKETIQECVSEYISFITG 105

Query: 133 EANERCHREQRKTVTAEDVLWAMGKLGFDNYIEPLTVFLNRYRESESIR 182
           EANERC REQRKT+TAEDVLWAM KLGFD+YIEPLT++L+RYRE E  R
Sbjct: 106 EANERCQREQRKTITAEDVLWAMSKLGFDDYIEPLTLYLHRYRELEGER 152

[Close]
BLAST of CSPI03G04270 vs. TAIR10
Match: AT5G47640.1 (AT5G47640.1 nuclear factor Y, subunit B2)

HSP 1 Score: 138.3 bits (347), Expect = 6.3e-33
Identity = 79/159 (49.69%), Postives = 106/159 (66.67%), Query Frame = 1 

Query: 75  NNNEQNQQCMV--REQDQYMPMANVIRIMKRILPSHAKISDDTKETIQECVSEYISFITS 134
           N N QN Q  +  REQD+++P+ANV RIMK+ LP++AKIS D KET+QECVSE+ISF+T 
Sbjct: 13  NGNNQNGQSSLSPREQDRFLPIANVSRIMKKALPANAKISKDAKETMQECVSEFISFVTG 72

Query: 135 EANERCHREQRKTVTAEDVLWAMGKLGFDNYIEPLTVFLNRYRESESIRTEPMLRRNVGY 194
           EA+++C +E+RKT+  +D+LWAM  LGF++Y+EPL V+L R+RE E  RT        G 
Sbjct: 73  EASDKCQKEKRKTINGDDLLWAMTTLGFEDYVEPLKVYLQRFREIEGERT--------GL 132

Query: 195 G-AQVGMISPYGQAFQIGQVPAGMFDAM---GGYYGGGG 228
           G  Q G           G+V     DA+   GG+YGGGG
Sbjct: 133 GRPQTG-----------GEVGEHQRDAVGDGGGFYGGGG 152

[Close]
BLAST of CSPI03G04270 vs. TAIR10
Match: AT4G14540.1 (AT4G14540.1 nuclear factor Y, subunit B3)

HSP 1 Score: 136.7 bits (343), Expect = 1.8e-32
Identity = 72/137 (52.55%), Postives = 95/137 (69.34%), Query Frame = 1 

Query: 86  REQDQYMPMANVIRIMKRILPSHAKISDDTKETIQECVSEYISFITSEANERCHREQRKT 145
           REQD+++P+ANV RIMK+ LP++AKIS D KET+QECVSE+ISFIT EA+++C RE+RKT
Sbjct: 20  REQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVSEFISFITGEASDKCQREKRKT 79

Query: 146 VTAEDVLWAMGKLGFDNYIEPLTVFLNRYRESESIRTEPMLRR--NVGYGAQVGMISPYG 205
           +  +D+LWAM  LGF++Y+EPL V+L +YRE E  +T    R+    G G   G  S  G
Sbjct: 80  INGDDLLWAMTTLGFEDYVEPLKVYLQKYREVEGEKTTTAGRQGDKEGGGGGGGAGSGSG 139

Query: 206 QAFQIGQVPAGMFDAMG 221
            A   G    GM   MG
Sbjct: 140 GAPMYG---GGMVTTMG 153

[Close]
BLAST of CSPI03G04270 vs. TAIR10
Match: AT3G53340.1 (AT3G53340.1 nuclear factor Y, subunit B10)

HSP 1 Score: 133.7 bits (335), Expect = 1.6e-31
Identity = 68/135 (50.37%), Postives = 92/135 (68.15%), Query Frame = 1 

Query: 85  VREQDQYMPMANVIRIMKRILPSHAKISDDTKETIQECVSEYISFITSEANERCHREQRK 144
           VREQD+++P+AN+ RIMKR LP + KI+ D KET+QECVSE+ISF+TSEA+++C RE+RK
Sbjct: 27  VREQDRFLPIANISRIMKRGLPLNGKIAKDAKETMQECVSEFISFVTSEASDKCQREKRK 86

Query: 145 TVTAEDVLWAMGKLGFDNYIEPLTVFLNRYRESE-----SIRTEPMLRRNVGYGAQVGMI 204
           T+  +D+LWAM  LGF++YI+PL V+L RYRE E     S +      +  G  +QV   
Sbjct: 87  TINGDDLLWAMATLGFEDYIDPLKVYLMRYREMEGDTKGSGKGGESSAKRDGQPSQVSQF 146

Query: 205 SPYGQAFQIGQVPAG 215
           S   Q     Q P G
Sbjct: 147 SQVPQQGSFSQGPYG 161

[Close]
BLAST of CSPI03G04270 vs. NCBI nr
Match: gi|700200905|gb|KGN56038.1| (hypothetical protein Csa_3G049440 [Cucumis sativus])

HSP 1 Score: 457.2 bits (1175), Expect = 1.7e-125
Identity = 233/239 (97.49%), Postives = 235/239 (98.33%), Query Frame = 1 

Query: 1   MKEGNEYGYFYVYVILMNLNLKYLCRYSGLNTMLTPNNATSINSNPNTNHHLSNGGTSIG 60
           MKEGNEYGYFYVYVILMNLNLKYLCRYSGLNTMLTPNN TSINSNPNTN HLSNGGTSIG
Sbjct: 1   MKEGNEYGYFYVYVILMNLNLKYLCRYSGLNTMLTPNNPTSINSNPNTNQHLSNGGTSIG 60

Query: 61  NSSATSPLSVAATANNNEQNQQCMVREQDQYMPMANVIRIMKRILPSHAKISDDTKETIQ 120
           NSSATSPLSVAATANNNEQNQQC+VREQDQYMPMANVIRIMKRILPSHAKISDDTKETIQ
Sbjct: 61  NSSATSPLSVAATANNNEQNQQCLVREQDQYMPMANVIRIMKRILPSHAKISDDTKETIQ 120

Query: 121 ECVSEYISFITSEANERCHREQRKTVTAEDVLWAMGKLGFDNYIEPLTVFLNRYRESESI 180
           ECVSEYISFITSEANERC REQRKTVTAEDVLWAMGKLGFD+YIEPLTVFLNRYRESESI
Sbjct: 121 ECVSEYISFITSEANERCQREQRKTVTAEDVLWAMGKLGFDDYIEPLTVFLNRYRESESI 180

Query: 181 RTEPMLRRNVGYGAQVGMISPYGQAFQIGQVPAGMFDAMGGYYGGGGSGGPSAGNNSQL 240
           RTEPMLRRNV YGAQVGMISPYGQAFQIGQVPAGMFDAMGGYYGGGGSGGPSAGNNSQL
Sbjct: 181 RTEPMLRRNVDYGAQVGMISPYGQAFQIGQVPAGMFDAMGGYYGGGGSGGPSAGNNSQL 239

[Close]
BLAST of CSPI03G04270 vs. NCBI nr
Match: gi|700200906|gb|KGN56039.1| (hypothetical protein Csa_3G055940 [Cucumis sativus])

HSP 1 Score: 369.0 bits (946), Expect = 6.3e-99
Identity = 194/239 (81.17%), Postives = 200/239 (83.68%), Query Frame = 1 

Query: 1   MKEGNEYGYFYVYVILMNLNLKYLCRYSGLNTMLTPNNATSINSNPNTNHHLSNGGTSIG 60
           +KEGNEYGYFYVYV+LMNLNL+YLCRYS LN MLTPNN  SIN+NPNTNHHLS+GGTSIG
Sbjct: 12  IKEGNEYGYFYVYVLLMNLNLQYLCRYSELNMMLTPNNPNSINNNPNTNHHLSDGGTSIG 71

Query: 61  NSSATSPLSVAATANNNEQNQQCMVREQDQYMPMANVIRIMKRILPSHAKISDDTKETIQ 120
           NSSATSPLSVAATANNNEQNQQC+VREQDQYMPMAN                        
Sbjct: 72  NSSATSPLSVAATANNNEQNQQCLVREQDQYMPMAN------------------------ 131

Query: 121 ECVSEYISFITSEANERCHREQRKTVTAEDVLWAMGKLGFDNYIEPLTVFLNRYRESESI 180
                YISFITSEANERC REQRKTVTAEDVLWAMGKLGFDNYIEPLTVFLNRYRESESI
Sbjct: 132 -----YISFITSEANERCQREQRKTVTAEDVLWAMGKLGFDNYIEPLTVFLNRYRESESI 191

Query: 181 RTEPMLRRNVGYGAQVGMISPYGQAFQIGQVPAGMFDAMGGYYGGGGSGGPSAGNNSQL 240
           RTEPMLRRNV YG QVGMISPYGQAFQIG VPAGMFDAMGGYYGGGGS GPS GNNSQL
Sbjct: 192 RTEPMLRRNVDYGPQVGMISPYGQAFQIGHVPAGMFDAMGGYYGGGGSCGPSTGNNSQL 221

[Close]
BLAST of CSPI03G04270 vs. NCBI nr
Match: gi|659096616|ref|XP_008449191.1| (PREDICTED: nuclear transcription factor Y subunit B-9 [Cucumis melo])

HSP 1 Score: 364.8 bits (935), Expect = 1.2e-97
Identity = 187/209 (89.47%), Postives = 196/209 (93.78%), Query Frame = 1 

Query: 33  MLTPNNATSINSNPNTNHHLSNGGTSIGNSSATSPLSVAATANNNEQNQQCMVREQDQYM 92
           M+TPN ATSIN+ PN+NHHLSNGGT+ GNSSATSPLSVAATANNNEQNQQC+VREQDQYM
Sbjct: 2   MVTPNKATSINNTPNSNHHLSNGGTATGNSSATSPLSVAATANNNEQNQQCVVREQDQYM 61

Query: 93  PMANVIRIMKRILPSHAKISDDTKETIQECVSEYISFITSEANERCHREQRKTVTAEDVL 152
           P+ANVIRIM+RILPSHAKISDD KETIQECVSEYISFIT EANERC REQRKTVTAEDVL
Sbjct: 62  PIANVIRIMRRILPSHAKISDDAKETIQECVSEYISFITGEANERCQREQRKTVTAEDVL 121

Query: 153 WAMGKLGFDNYIEPLTVFLNRYRESES--IRTEPMLRRNVGYGAQVGMISPYGQAFQIGQ 212
           WAMGKLGFD+YIEPLTVFLNRYRESES  IRTEP+LRRNV YG QVGMISPYGQAFQIG 
Sbjct: 122 WAMGKLGFDDYIEPLTVFLNRYRESESDRIRTEPILRRNVDYGPQVGMISPYGQAFQIGH 181

Query: 213 VPAGMFDAMGGYYGGGGSGGPSAGNNSQL 240
           VPAGMFDAMGGYYGGGG GGPSAGN SQL
Sbjct: 182 VPAGMFDAMGGYYGGGGGGGPSAGNGSQL 210

[Close]
BLAST of CSPI03G04270 vs. NCBI nr
Match: gi|728844156|gb|KHG23599.1| (Nuclear transcription factor Y subunit B-9 -like protein [Gossypium arboreum])

HSP 1 Score: 228.4 bits (581), Expect = 1.3e-56
Identity = 137/243 (56.38%), Postives = 165/243 (67.90%), Query Frame = 1 

Query: 1   MKEGNEYGYFYVYVILMNLNLKYLCRYSGLNTMLTPNNATSINSNPNTNHHLSNGGTSIG 60
           M+ G+ +  FY          K+    SGL+ M    +  S NSN NTN  ++N   + G
Sbjct: 1   MERGDGFNRFY----------KHAKSSSGLSIM----HGDSSNSNTNTNTIINNNAANNG 60

Query: 61  NSSATSPLSVAATANNNEQNQQCMVREQDQYMPMANVIRIMKRILPSHAKISDDTKETIQ 120
           N+           AN+N     CMVREQDQYMP+ANVIRIM+RILP+HAKISDD KETIQ
Sbjct: 61  NA-----------ANSNTMPPPCMVREQDQYMPIANVIRIMRRILPTHAKISDDAKETIQ 120

Query: 121 ECVSEYISFITSEANERCHREQRKTVTAEDVLWAMGKLGFDNYIEPLTVFLNRYRESE-- 180
           ECVSEYISFIT EANERC REQRKT+TAEDVLWAMGKLGFD+Y+EPLTVFLNRYRE+E  
Sbjct: 121 ECVSEYISFITGEANERCQREQRKTITAEDVLWAMGKLGFDDYVEPLTVFLNRYRENENE 180

Query: 181 --SIRTEPMLRRNVGYGAQVGMISPYGQAFQIGQVPAGMFD---AMGGYY---GGGGSGG 234
             S+R+EPML+R + YG  + M++PYG  F +G    G+FD   AMGGY      GG GG
Sbjct: 181 RTSLRSEPMLKRGIDYGPSM-MMAPYGAGFHVGH-QQGIFDGATAMGGYMRDGSSGGGGG 216

[Close]
BLAST of CSPI03G04270 vs. NCBI nr
Match: gi|823266462|ref|XP_012465928.1| (PREDICTED: nuclear transcription factor Y subunit B-9 [Gossypium raimondii])

HSP 1 Score: 221.9 bits (564), Expect = 1.2e-54
Identity = 125/198 (63.13%), Postives = 148/198 (74.75%), Query Frame = 1 

Query: 50  HHLSNGGTSI----GNSSATSPLSVAATANNNEQNQQCMVREQDQYMPMANVIRIMKRIL 109
           H  S+ G SI     ++S T+ +     AN+N     CMVREQDQYMP+ANVIRIM+RIL
Sbjct: 13  HAKSSSGLSIMHGDSSNSNTNTIINNNAANSNTMPPPCMVREQDQYMPIANVIRIMRRIL 72

Query: 110 PSHAKISDDTKETIQECVSEYISFITSEANERCHREQRKTVTAEDVLWAMGKLGFDNYIE 169
           P+HAKISDD KETIQECVSEYISFIT EANERC REQRKT+TAEDVLWAMGKLGFD+Y+E
Sbjct: 73  PTHAKISDDAKETIQECVSEYISFITGEANERCQREQRKTITAEDVLWAMGKLGFDDYVE 132

Query: 170 PLTVFLNRYRESE----SIRTEPMLRRNVGYGAQVGMISPYGQAFQIGQVPAGMFD---A 229
           PLTVFLNRYRE+E    S+R+EPML+R + YG  + M++PYG  F +G    G+FD   A
Sbjct: 133 PLTVFLNRYRENENERTSLRSEPMLKRGIDYGPSM-MMAPYGAGFHVGH-QQGIFDGATA 192

Query: 230 MGGYY---GGGGSGGPSA 234
           MGGY      GG GGPS+
Sbjct: 193 MGGYMRDGSSGGGGGPSS 208

The following BLAST results are available for this feature:
BLAST of CSPI03G04270 vs. Swiss-Prot
Match NameE-valueIdentityDescription
NFYB9_ARATH4.0e-4557.78Nuclear transcription factor Y subunit B-9 OS=Arabidopsis thaliana GN=NFYB9 PE=1... [more]
NFYB6_ARATH1.8e-4277.06Nuclear transcription factor Y subunit B-6 OS=Arabidopsis thaliana GN=NFYB6 PE=1... [more]
NFYB2_ARATH1.1e-3149.69Nuclear transcription factor Y subunit B-2 OS=Arabidopsis thaliana GN=NFYB2 PE=2... [more]
NFYB3_ARATH3.3e-3152.55Nuclear transcription factor Y subunit B-3 OS=Arabidopsis thaliana GN=NFYB3 PE=2... [more]
NFYB3_ORYSJ9.5e-3161.70Nuclear transcription factor Y subunit B-3 OS=Oryza sativa subsp. japonica GN=NF... [more]
BLAST of CSPI03G04270 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0L2U1_CUCSA1.2e-12597.49Uncharacterized protein OS=Cucumis sativus GN=Csa_3G049440 PE=4 SV=1[more]
A0A0A0L4W1_CUCSA4.4e-9981.17Uncharacterized protein OS=Cucumis sativus GN=Csa_3G055940 PE=4 SV=1[more]
A0A0B0PGM2_GOSAR9.2e-5756.38Nuclear transcription factor Y subunit B-9-like protein OS=Gossypium arboreum GN... [more]
A0A0D2SEP9_GOSRA8.6e-5563.13Uncharacterized protein OS=Gossypium raimondii GN=B456_013G152500 PE=4 SV=1[more]
A0A0A0L645_CUCSA8.6e-5589.68Uncharacterized protein OS=Cucumis sativus GN=Csa_3G048940 PE=4 SV=1[more]
BLAST of CSPI03G04270 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G21970.12.2e-4657.78 Histone superfamily protein[more]
AT5G47670.11.0e-4377.06 nuclear factor Y, subunit B6[more]
AT5G47640.16.3e-3349.69 nuclear factor Y, subunit B2[more]
AT4G14540.11.8e-3252.55 nuclear factor Y, subunit B3[more]
AT3G53340.11.6e-3150.37 nuclear factor Y, subunit B10[more]
BLAST of CSPI03G04270 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|700200905|gb|KGN56038.1|1.7e-12597.49hypothetical protein Csa_3G049440 [Cucumis sativus][more]
gi|700200906|gb|KGN56039.1|6.3e-9981.17hypothetical protein Csa_3G055940 [Cucumis sativus][more]
gi|659096616|ref|XP_008449191.1|1.2e-9789.47PREDICTED: nuclear transcription factor Y subunit B-9 [Cucumis melo][more]
gi|728844156|gb|KHG23599.1|1.3e-5656.38Nuclear transcription factor Y subunit B-9 -like protein [Gossypium arboreum][more]
gi|823266462|ref|XP_012465928.1|1.2e-5463.13PREDICTED: nuclear transcription factor Y subunit B-9 [Gossypium raimondii][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR003956Transcrpt_fac_NFYB/HAP3_CS
IPR003958CBFA_NFYB_domain
IPR009072Histone-fold
Vocabulary: Biological Process
TermDefinition
GO:0006355regulation of transcription, DNA-templated
Vocabulary: Cellular Component
TermDefinition
GO:0005634nucleus
Vocabulary: Molecular Function
TermDefinition
GO:0043565sequence-specific DNA binding
GO:0046982protein heterodimerization activity
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009738 abscisic acid-activated signaling pathway
biological_process GO:0045893 positive regulation of transcription, DNA-templated
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
cellular_component GO:0005667 transcription factor complex
molecular_function GO:0033613 activating transcription factor binding
molecular_function GO:0046982 protein heterodimerization activity
molecular_function GO:0043565 sequence-specific DNA binding
molecular_function GO:0003700 transcription factor activity, sequence-specific DNA binding
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI03G04270.1CSPI03G04270.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003956Transcription factor, NFYB/HAP3, conserved sitePROSITEPS00685NFYB_HAP3coord: 122..138
scor
IPR003958Transcription factor CBF/NF-Y/archaeal histone domainPFAMPF00808CBFD_NFYB_HMFcoord: 91..155
score: 1.5
IPR009072Histone-foldGENE3DG3DSA:1.10.20.10coord: 79..182
score: 1.5
IPR009072Histone-foldunknownSSF47113Histone-foldcoord: 88..183
score: 9.19
NoneNo IPR availablePRINTSPR00615CCAATSUBUNTAcoord: 157..175
score: 5.2E-17coord: 119..137
score: 5.2E-17coord: 138..156
score: 5.2
NoneNo IPR availablePANTHERPTHR11064CCAAT-BINDING TRANSCRIPTION FACTOR-RELATEDcoord: 62..222
score: 6.1
NoneNo IPR availablePANTHERPTHR11064:SF20NUCLEAR TRANSCRIPTION FACTOR Y SUBUNIT B-6-RELATEDcoord: 62..222
score: 6.1