CSPI03G04010 (gene) Wild cucumber (PI 183967) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CSPI03G04010
Type	gene
Organism	Cucumis sativus (Wild cucumber (PI 183967))
Description	Caffeic acid O-methyltransferase
Location	Chr3 : 3320470 .. 3321033 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTTCCTCCGACAACGACACCGCCGTCGAACAGCAACAACATTATGCTTACGCAGGACACCTCGTTACCTTATCAGTCCTACCCATGACACTCCAAGCAGTGTTCGAGCTTGGTGTGTTCGAGATCTTGGCAAAGGCTGGGGATGGGGCCAACCTCTCTCCAGCAGAGATAGCGGCTGAGATAACCACTACAAACCCCAACGCAGCATTGATGCTTGATCGGATGTTGAGGCTTTTGGCATGCCACTCGGTGGTGGGATGCTCTATAGTTTCTGATAAAGATGGGAATGTGCAAAGGCTTTATAGCCTTACTCCGATTTCTAAATGTTATGTTCGAAATGAAGATGGTGTTTCGTTGGGTCCAATGTTATCATTGATTCAAGATAAAGTGTTTCTGCAAAGCTGGTTTGCTCTCCTTAATTTCTTTATGCTTTTTAATATATATTTTTTTCGTTTAATAGAAGGGAAATTAGTAGGAATAGCGAAGATAAAATATAAATGGAGATATGTTATATTAGTGAGAAATTATGTAAGTACAGCTATGTTTGAGAAATTAGAGAGT

mRNA sequence

ATGGCTTCCTCCGACAACGACACCGCCGTCGAACAGCAACAACATTATGCTTACGCAGGACACCTCGTTACCTTATCAGTCCTACCCATGACACTCCAAGCAGTGTTCGAGCTTGGTGTGTTCGAGATCTTGGCAAAGGCTGGGGATGGGGCCAACCTCTCTCCAGCAGAGATAGCGGCTGAGATAACCACTACAAACCCCAACGCAGCATTGATGCTTGATCGGATGTTGAGGCTTTTGGCATGCCACTCGGTGGTGGGATGCTCTATAGTTTCTGATAAAGATGGGAATGTGCAAAGGCTTTATAGCCTTACTCCGATTTCTAAATGTTATGTTCGAAATGAAGATGGTGTTTCGTTGGGTCCAATGTTATCATTGATTCAAGATAAAGTGTTTCTGCAAAGCTGGTTTGCTCTCCTTAATTTCTTTATGCTTTTTAATATATATTTTTTTCGTTTAATAGAAGGGAAATTAGTAGGAATAGCGAAGATAAAATATAAATGGAGATATGTTATATTAGTGAGAAATTATGTAAGTACAGCTATGTTTGAGAAATTAGAGAGT

Coding sequence (CDS)

ATGGCTTCCTCCGACAACGACACCGCCGTCGAACAGCAACAACATTATGCTTACGCAGGACACCTCGTTACCTTATCAGTCCTACCCATGACACTCCAAGCAGTGTTCGAGCTTGGTGTGTTCGAGATCTTGGCAAAGGCTGGGGATGGGGCCAACCTCTCTCCAGCAGAGATAGCGGCTGAGATAACCACTACAAACCCCAACGCAGCATTGATGCTTGATCGGATGTTGAGGCTTTTGGCATGCCACTCGGTGGTGGGATGCTCTATAGTTTCTGATAAAGATGGGAATGTGCAAAGGCTTTATAGCCTTACTCCGATTTCTAAATGTTATGTTCGAAATGAAGATGGTGTTTCGTTGGGTCCAATGTTATCATTGATTCAAGATAAAGTGTTTCTGCAAAGCTGGTTTGCTCTCCTTAATTTCTTTATGCTTTTTAATATATATTTTTTTCGTTTAATAGAAGGGAAATTAGTAGGAATAGCGAAGATAAAATATAAATGGAGATATGTTATATTAGTGAGAAATTATGTAAGTACAGCTATGTTTGAGAAATTAGAGAGT

Homology

[Close]

BLAST of CSPI03G04010 vs. Swiss-Prot
Match: COMT1_CAPAN (Caffeic acid 3-O-methyltransferase OS=Capsicum annuum GN=COMT PE=2 SV=2)

HSP 1 Score: 136.3 bits (342), Expect = 3.3e-31
Identity = 66/139 (47.48%), Postives = 98/139 (70.50%), Query Frame = 1


Query: 1   MASSDNDTAVEQQQHYAYAGHLVTLSVLPMTLQAVFELGVFEILAKAGDGANLSPAEIAA 60
           M S++ +    + + + +A  L + SVLPM L++  EL + EI+AKAG GA +SP+E+AA
Sbjct: 1   MDSTNQNLTQTEDEAFLFAMQLASASVLPMVLKSALELDLLEIMAKAGPGAAISPSELAA 60

Query: 61  EITTTNPNAALMLDRMLRLLACHSVVGCSIVSDKDGNVQRLYSLTPISKCYVRNEDGVSL 120
           ++ T NP A +MLDRMLRLLA +SV+ C++ +  DG V+RLYSL P+ K   +N DGVS+
Sbjct: 61  QLPTKNPEAPVMLDRMLRLLATYSVLNCTLRTLPDGRVERLYSLAPVCKLLTKNADGVSV 120

Query: 121 GPMLSLIQDKVFLQSWFAL 140
            P+L + QDKV ++SW+ L
Sbjct: 121 APLLLMNQDKVLMESWYHL 139

[Close]

BLAST of CSPI03G04010 vs. Swiss-Prot
Match: COMT1_MEDSA (Caffeic acid 3-O-methyltransferase OS=Medicago sativa PE=1 SV=1)

HSP 1 Score: 136.0 bits (341), Expect = 4.4e-31
Identity = 65/132 (49.24%), Postives = 95/132 (71.97%), Query Frame = 1


Query: 8   TAVEQQQHYAYAGHLVTLSVLPMTLQAVFELGVFEILAKAGDGANLSPAEIAAEITTTNP 67
           T +  ++   +A  L + SVLPM L++  EL + EI+AKAG GA +SP EIA+++ TTNP
Sbjct: 12  THISDEEANLFAMQLASASVLPMILKSALELDLLEIIAKAGPGAQISPIEIASQLPTTNP 71

Query: 68  NAALMLDRMLRLLACHSVVGCSIVSDKDGNVQRLYSLTPISKCYVRNEDGVSLGPMLSLI 127
           +A +MLDRMLRLLAC+ ++ CS+ + +DG VQRLY L  ++K  V+NEDGVS+  +  + 
Sbjct: 72  DAPVMLDRMLRLLACYIILTCSVRTQQDGKVQRLYGLATVAKYLVKNEDGVSISALNLMN 131

Query: 128 QDKVFLQSWFAL 140
           QDKV ++SW+ L
Sbjct: 132 QDKVLMESWYHL 143

[Close]

BLAST of CSPI03G04010 vs. Swiss-Prot
Match: COMT1_CLABR (Caffeic acid 3-O-methyltransferase OS=Clarkia breweri GN=COMT PE=1 SV=1)

HSP 1 Score: 134.8 bits (338), Expect = 9.7e-31
Identity = 71/135 (52.59%), Postives = 94/135 (69.63%), Query Frame = 1


Query: 8   TAVEQQQHYAYAGHLVTLSVLPMTLQAVFELGVFEILAKA---GDGANLSPAEIAAEITT 67
           T V  ++   +A  L + SVLPM L+A  EL V EI+AK+   G GA +SPAEIAA++ T
Sbjct: 14  THVSDEEANLFAMQLASASVLPMVLKAAIELDVLEIMAKSIPHGSGAYISPAEIAAQLPT 73

Query: 68  TNPNAALMLDRMLRLLACHSVVGCSIVSDKDGNVQRLYSLTPISKCYVRNEDGVSLGPML 127
           TNP+A +MLDR+LRLLA +SVV CS+    DG V+RLY L P+ K   +NEDGVSL P+ 
Sbjct: 74  TNPDAPVMLDRVLRLLASYSVVTCSLRELPDGKVERLYGLAPVCKFLTKNEDGVSLAPLC 133

Query: 128 SLIQDKVFLQSWFAL 140
            + QDKV ++SW+ L
Sbjct: 134 LMNQDKVLMESWYYL 148

[Close]

BLAST of CSPI03G04010 vs. Swiss-Prot
Match: COMT1_CAPCH (Caffeic acid 3-O-methyltransferase OS=Capsicum chinense GN=COMT PE=2 SV=1)

HSP 1 Score: 132.9 bits (333), Expect = 3.7e-30
Identity = 65/139 (46.76%), Postives = 95/139 (68.35%), Query Frame = 1


Query: 1   MASSDNDTAVEQQQHYAYAGHLVTLSVLPMTLQAVFELGVFEILAKAGDGANLSPAEIAA 60
           M S +      + + + +A  L + SVLPM L+A  EL + EI+AK+G GA +SP+E+AA
Sbjct: 1   MGSINQSLTQTEDEAFVFAMQLASASVLPMVLKATVELDLLEIMAKSGPGAFISPSELAA 60

Query: 61  EITTTNPNAALMLDRMLRLLACHSVVGCSIVSDKDGNVQRLYSLTPISKCYVRNEDGVSL 120
           ++ T NP A +MLDRM RLLA +SV+ C++ +  DG V+RLYSL P+ K   +N DGVS+
Sbjct: 61  QLPTKNPEAPVMLDRMFRLLATYSVLNCTLRTLPDGRVERLYSLAPVCKFLTKNGDGVSI 120

Query: 121 GPMLSLIQDKVFLQSWFAL 140
            P+L + QDKV ++SW+ L
Sbjct: 121 APILLMNQDKVLMESWYHL 139

[Close]

BLAST of CSPI03G04010 vs. Swiss-Prot
Match: COMT1_POPTM (Caffeic acid 3-O-methyltransferase 1 OS=Populus tremuloides GN=OMT1 PE=1 SV=1)

HSP 1 Score: 129.4 bits (324), Expect = 4.1e-29
Identity = 64/132 (48.48%), Postives = 91/132 (68.94%), Query Frame = 1


Query: 8   TAVEQQQHYAYAGHLVTLSVLPMTLQAVFELGVFEILAKAGDGANLSPAEIAAEITTTNP 67
           T V  ++ + +A  L + SVLPM L+   EL + EI+AKAG GA LS +EIA+ + T NP
Sbjct: 12  TQVSDEEAHLFAMQLASASVLPMILKTAIELDLLEIMAKAGPGAFLSTSEIASHLPTKNP 71

Query: 68  NAALMLDRMLRLLACHSVVGCSIVSDKDGNVQRLYSLTPISKCYVRNEDGVSLGPMLSLI 127
           +A +MLDR+LRLLA +S++ CS+    DG V+RLY L P+ K   +NEDGVS+ P+  + 
Sbjct: 72  DAPVMLDRILRLLASYSILTCSLKDLPDGKVERLYGLAPVCKFLTKNEDGVSVSPLCLMN 131

Query: 128 QDKVFLQSWFAL 140
           QDKV ++SW+ L
Sbjct: 132 QDKVLMESWYYL 143

[Close]

BLAST of CSPI03G04010 vs. TrEMBL
Match: A0A061GSD8_THECC (Caffeic acid 3-O-methyltransferase 1 OS=Theobroma cacao GN=TCM_040759 PE=3 SV=1)

HSP 1 Score: 154.8 bits (390), Expect = 1.0e-34
Identity = 81/154 (52.60%), Postives = 111/154 (72.08%), Query Frame = 1


Query: 1   MASSDNDTAVEQQQHYAYAGHLVTLSVLPMTLQAVFELGVFEILAKAGDGANLSPAEIAA 60
           + S    +A + Q+ ++YA  +VT + LPM++ A  +L +FEI+AKAG  A LSP EIAA
Sbjct: 43  LCSPMESSAEQNQESFSYAIQIVTSNALPMSMHAAIQLQLFEIIAKAGPDAKLSPKEIAA 102

Query: 61  EITTTNPNAALMLDRMLRLLACHSVVGCSIVSDKDG-NVQRLYSLTPISKCYVRNEDGVS 120
           ++ T NP+A  MLDR+LR+LA H++VGCS+  D+ G N QRLYSLTP+SK +V NEDGVS
Sbjct: 103 QLRTKNPDAPSMLDRLLRVLASHNIVGCSVADDEQGNNPQRLYSLTPVSKFFVPNEDGVS 162

Query: 121 LGPMLSLIQDKVFLQSWFALLNFFMLFNIYFFRL 154
           LGP++SL+QDKVFL SW  L +  +   I F R+
Sbjct: 163 LGPLMSLLQDKVFLDSWSQLKDAILEGGIPFDRV 196

[Close]

BLAST of CSPI03G04010 vs. TrEMBL
Match: A0A061GUA5_THECC (Caffeic acid 3-O-methyltransferase 1 isoform 2 OS=Theobroma cacao GN=TCM_040763 PE=3 SV=1)

HSP 1 Score: 154.1 bits (388), Expect = 1.7e-34
Identity = 80/147 (54.42%), Postives = 109/147 (74.15%), Query Frame = 1


Query: 8   TAVEQQQHYAYAGHLVTLSVLPMTLQAVFELGVFEILAKAGDGANLSPAEIAAEITTTNP 67
           +A + Q+ ++YA  +VT + LPM++ A  +L +FEI+AKAG  A LSP EIAA++ T NP
Sbjct: 4   SAEQNQESFSYAIQIVTSNALPMSMHAAIQLQLFEIIAKAGPDAKLSPKEIAAQLRTKNP 63

Query: 68  NAALMLDRMLRLLACHSVVGCSIVSDKDG-NVQRLYSLTPISKCYVRNEDGVSLGPMLSL 127
           +A  MLDR+LR+LA H++VGCS+  D+ G N QRLYSLTP+SK +V NEDGVSLGP++SL
Sbjct: 64  DAPSMLDRLLRVLASHNIVGCSVADDEQGNNPQRLYSLTPVSKFFVPNEDGVSLGPLMSL 123

Query: 128 IQDKVFLQSWFALLNFFMLFNIYFFRL 154
           +QDKVFL SW  L +  +   I F R+
Sbjct: 124 LQDKVFLDSWSQLKDAILEGGIPFDRV 150

[Close]

BLAST of CSPI03G04010 vs. TrEMBL
Match: A0A061GTC8_THECC (Caffeic acid 3-O-methyltransferase 1 isoform 1 OS=Theobroma cacao GN=TCM_040763 PE=3 SV=1)

HSP 1 Score: 154.1 bits (388), Expect = 1.7e-34
Identity = 80/147 (54.42%), Postives = 109/147 (74.15%), Query Frame = 1


Query: 8   TAVEQQQHYAYAGHLVTLSVLPMTLQAVFELGVFEILAKAGDGANLSPAEIAAEITTTNP 67
           +A + Q+ ++YA  +VT + LPM++ A  +L +FEI+AKAG  A LSP EIAA++ T NP
Sbjct: 4   SAEQNQESFSYAIQIVTSNALPMSMHAAIQLQLFEIIAKAGPDAKLSPKEIAAQLRTKNP 63

Query: 68  NAALMLDRMLRLLACHSVVGCSIVSDKDG-NVQRLYSLTPISKCYVRNEDGVSLGPMLSL 127
           +A  MLDR+LR+LA H++VGCS+  D+ G N QRLYSLTP+SK +V NEDGVSLGP++SL
Sbjct: 64  DAPSMLDRLLRVLASHNIVGCSVADDEQGNNPQRLYSLTPVSKFFVPNEDGVSLGPLMSL 123

Query: 128 IQDKVFLQSWFALLNFFMLFNIYFFRL 154
           +QDKVFL SW  L +  +   I F R+
Sbjct: 124 LQDKVFLDSWSQLKDAILEGGIPFDRV 150

[Close]

BLAST of CSPI03G04010 vs. TrEMBL
Match: W9S415_9ROSA (Caffeic acid 3-O-methyltransferase OS=Morus notabilis GN=L484_019621 PE=3 SV=1)

HSP 1 Score: 151.0 bits (380), Expect = 1.5e-33
Identity = 79/135 (58.52%), Postives = 101/135 (74.81%), Query Frame = 1


Query: 11  EQQQHY-AYAGHLVTLSVLPMTLQAVFELGVFEILAKAGDGANLSPAEIAAEITTTNPNA 70
           EQ++H+ +YA  L T +VL M+LQ+ FELGVF+I+AKAG+GA LS  EIAA + TTNP A
Sbjct: 22  EQEEHFFSYAMQLATSTVLSMSLQSAFELGVFDIIAKAGEGAKLSSVEIAAHLPTTNPEA 81

Query: 71  ALMLDRMLRLLACHSVVGCSIVSDK---DGNVQRLYSLTPISKCYVRNEDGVSLGPMLSL 130
            +MLDR+LRLLA HSV+ CS+  D      N Q LYSL+P+SK +V NEDGVSLGP ++L
Sbjct: 82  PIMLDRILRLLASHSVLNCSLDGDNGDIGSNFQWLYSLSPVSKYFVTNEDGVSLGPFVAL 141

Query: 131 IQDKVFLQSWFALLN 142
           +QDKV  +SW  L N
Sbjct: 142 VQDKVSFESWPQLKN 156

[Close]

BLAST of CSPI03G04010 vs. TrEMBL
Match: W9SI12_9ROSA (Caffeic acid 3-O-methyltransferase OS=Morus notabilis GN=L484_013036 PE=3 SV=1)

HSP 1 Score: 150.6 bits (379), Expect = 1.9e-33
Identity = 78/145 (53.79%), Postives = 107/145 (73.79%), Query Frame = 1


Query: 11  EQQQHYAYAGHLVTLSVLPMTLQAVFELGVFEILAKAGDGANLSPAEIAAEITTTNPNAA 70
           E +++++YA  LV  +VL M+LQ   ELGVF+I+AKAG+GA LSPAEI A++TT NP A 
Sbjct: 24  EDKENFSYAMQLVFSTVLSMSLQTAIELGVFDIIAKAGEGAKLSPAEITAQMTTDNPEAP 83

Query: 71  LMLDRMLRLLACHSVVGCSIVSD--KDGNVQRLYSLTPISKCYVRNEDGVSLGPMLSLIQ 130
           +MLDR+LR+LA HS++ CS+V D   + + QRLYSL P++K +  NEDGVSLGP+++LIQ
Sbjct: 84  IMLDRILRVLASHSILRCSVVGDVNSESDFQRLYSLGPVAKYFATNEDGVSLGPLMALIQ 143

Query: 131 DKVFLQSWFALLNFFMLFNIYFFRL 154
           DK+FL SW  L    +   I F R+
Sbjct: 144 DKIFLDSWPQLKEAVLKGGIAFNRV 168

[Close]

BLAST of CSPI03G04010 vs. TAIR10
Match: AT5G54160.1 (AT5G54160.1 O-methyltransferase 1)

HSP 1 Score: 104.0 bits (258), Expect = 1.0e-22
Identity = 60/149 (40.27%), Postives = 91/149 (61.07%), Query Frame = 1


Query: 18  YAGHLVTLSVLPMTLQAVFELGVFEILAKAGDGANLSPAEIAAEITTTNPNAALMLDRML 77
           +A  L + SVLPM L++  EL + EI+AK  +G+ +SP EIA+++ T NP A +MLDR+L
Sbjct: 22  FAMQLASASVLPMALKSALELDLLEIMAK--NGSPMSPTEIASKLPTKNPEAPVMLDRIL 81

Query: 78  RLLACHSVVGCSIVSDKDGNVQRLYSLTPISKCYVRNEDGVSLGPMLSLIQDKVFLQSWF 137
           RLL  +SV+ CS        V+R+Y L P+ K   +NEDGVS+  +  + QDKV ++SW+
Sbjct: 82  RLLTSYSVLTCSNRKLSGDGVERIYGLGPVCKYLTKNEDGVSIAALCLMNQDKVLMESWY 141

Query: 138 ALLNFFMLFNIYFFRLIEGKLVGIAKIKY 167
            L +  +   I F      K  G++  +Y
Sbjct: 142 HLKDAILDGGIPF-----NKAYGMSAFEY 163

[Close]

BLAST of CSPI03G04010 vs. TAIR10
Match: AT1G77520.1 (AT1G77520.1 O-methyltransferase family protein)

HSP 1 Score: 96.3 bits (238), Expect = 2.2e-20
Identity = 51/134 (38.06%), Postives = 81/134 (60.45%), Query Frame = 1


Query: 10  VEQQQHYAYAGHLVTLSVLPMTLQAVFELGVFEILAKAGDGANLSPAEIAAEITT--TNP 69
           V+++     A  +V     PM L+A FELGV + +A AG+   LSP EIA  + T  TNP
Sbjct: 24  VDEKMVSLQAESIVNTVAFPMVLKAAFELGVIDTIAAAGNDTWLSPCEIACSLPTKPTNP 83

Query: 70  NAALMLDRMLRLLACHSVVGCSIV----SDKDGNVQRLYSLTPISKCYVRNEDGV-SLGP 129
            A ++LDRML LL  HS++ C ++    + + G ++R+Y+  P+ K ++R+ DG  SL P
Sbjct: 84  EAPVLLDRMLSLLVSHSILKCRMIETGENGRTGKIERVYAAEPVCKYFLRDSDGTGSLVP 143

Query: 130 MLSLIQDKVFLQSW 137
           +  L+  +VF ++W
Sbjct: 144 LFMLLHTQVFFKTW 157

[Close]

BLAST of CSPI03G04010 vs. TAIR10
Match: AT1G77530.1 (AT1G77530.1 O-methyltransferase family protein)

HSP 1 Score: 93.6 bits (231), Expect = 1.4e-19
Identity = 50/134 (37.31%), Postives = 81/134 (60.45%), Query Frame = 1


Query: 10  VEQQQHYAYAGHLVTLSVLPMTLQAVFELGVFEILAKAGDGANLSPAEIAAEITT--TNP 69
           ++++     A  +V     PM L+A  ELGV + +A A +G  LSP+EIA  +    TNP
Sbjct: 24  IDEKMVSLQAESIVNAVAFPMVLKAALELGVIDTIAAASNGTWLSPSEIAVSLPNKPTNP 83

Query: 70  NAALMLDRMLRLLACHSVVGCSIVSDKD----GNVQRLYSLTPISKCYVRNEDGV-SLGP 129
            A ++LDRMLRLL  HS++ C +V  ++    G ++R+Y+  PI K ++++ DG  SL  
Sbjct: 84  EAPVLLDRMLRLLVSHSILKCCMVESRENGQTGKIERVYAAEPICKYFLKDSDGSGSLSS 143

Query: 130 MLSLIQDKVFLQSW 137
           +L L+  +V L++W
Sbjct: 144 LLLLLHSQVILKTW 157

[Close]

BLAST of CSPI03G04010 vs. TAIR10
Match: AT1G33030.1 (AT1G33030.1 O-methyltransferase family protein)

HSP 1 Score: 82.4 bits (202), Expect = 3.2e-16
Identity = 48/131 (36.64%), Postives = 82/131 (62.60%), Query Frame = 1


Query: 10  VEQQQHYAYAGHLVTLSVLPMTLQAVFELGVFEILAKAGDGANLSPAEIAAEITTTNPNA 69
           +E+Q   +YA  L + SVLPM L+   +LG+F+ILA++G  +      + +  T  + ++
Sbjct: 1   MEEQNLSSYAMILSSSSVLPMVLKTAIDLGLFDILAESGPSSASQIFSLLSNETKKHHDS 60

Query: 70  ALMLDRMLRLLACHSVVGCSIVSDKDGNVQRLYSLTPISKCYVRNED-GVSLGPMLSLIQ 129
           +L ++R+LR LA +S++ CS VS + G    +Y L P++K + +N++ G SL PM++L Q
Sbjct: 61  SL-VNRILRFLASYSILTCS-VSTEHGEPFAIYGLAPVAKYFTKNQNGGGSLAPMVNLFQ 120

Query: 130 DKVFLQSWFAL 140
           DKV    W+ L
Sbjct: 121 DKVVTDMWYNL 129

[Close]

BLAST of CSPI03G04010 vs. TAIR10
Match: AT1G51990.1 (AT1G51990.1 O-methyltransferase family protein)

HSP 1 Score: 80.9 bits (198), Expect = 9.4e-16
Identity = 43/123 (34.96%), Postives = 69/123 (56.10%), Query Frame = 1


Query: 1   MASSDNDTAVEQQQHYAYAGHLVTLSVLPMTLQAVFELGVFEILAKAGD-GANLSPAEIA 60
           M S        +++    A  L  L+ +P  ++   EL +FEI+AKA   G+ LSP ++A
Sbjct: 1   MISLQTSGGSSEEEDMLLAIQLGGLNFVPYIVKTARELDLFEIMAKARPLGSYLSPVDLA 60

Query: 61  AEITTTNPNAALMLDRMLRLLACHSVVGCSIVSDKDGNVQRLYSLTPISKCYVRNEDGVS 120
           +     NP+A +M+DR+LR L  +SV  C +V D++G   R Y L  + K  +++EDG S
Sbjct: 61  SMAAPKNPHAPMMIDRLLRFLVAYSVCTCKLVKDEEGRESRAYGLGKVGKKLIKDEDGFS 120

Query: 121 LGP 123
           + P
Sbjct: 121 IAP 123

[Close]

BLAST of CSPI03G04010 vs. NCBI nr
Match: gi|778675776|ref|XP_004150935.2| (PREDICTED: caffeic acid 3-O-methyltransferase-like [Cucumis sativus])

HSP 1 Score: 268.9 bits (686), Expect = 6.9e-69
Identity = 137/141 (97.16%), Postives = 138/141 (97.87%), Query Frame = 1


Query: 1   MASSDNDTAVEQQQHYAYAGHLVTLSVLPMTLQAVFELGVFEILAKAGDGANLSPAEIAA 60
           MASSDNDTAVEQQQHYAYAGHLVTLSVLPMTLQAVFELGVFEILAKAGDGANLSPAEIAA
Sbjct: 1   MASSDNDTAVEQQQHYAYAGHLVTLSVLPMTLQAVFELGVFEILAKAGDGANLSPAEIAA 60

Query: 61  EITTTNPNAALMLDRMLRLLACHSVVGCSIVSDKDGNVQRLYSLTPISKCYVRNEDGVSL 120
           EITTTNP+AALMLDRMLRLLACHSVVGCSIVSDKDGNVQRLYSLTPISKCYVRNEDGVSL
Sbjct: 61  EITTTNPDAALMLDRMLRLLACHSVVGCSIVSDKDGNVQRLYSLTPISKCYVRNEDGVSL 120

Query: 121 GPMLSLIQDKVFLQSWFALLN 142
           GPMLSLIQDKVFLQSW  L N
Sbjct: 121 GPMLSLIQDKVFLQSWSELKN 141

[Close]

BLAST of CSPI03G04010 vs. NCBI nr
Match: gi|778675782|ref|XP_004148264.2| (PREDICTED: caffeic acid 3-O-methyltransferase [Cucumis sativus])

HSP 1 Score: 250.8 bits (639), Expect = 1.9e-63
Identity = 130/141 (92.20%), Postives = 133/141 (94.33%), Query Frame = 1


Query: 1   MASSDNDTAVEQQQHYAYAGHLVTLSVLPMTLQAVFELGVFEILAKAGDGANLSPAEIAA 60
           MASSDN+TAVEQQQ+YAYA  LVTLSVLPMTLQAVFELGVFEILAKAGDGANLSPAEIAA
Sbjct: 1   MASSDNNTAVEQQQNYAYAAQLVTLSVLPMTLQAVFELGVFEILAKAGDGANLSPAEIAA 60

Query: 61  EITTTNPNAALMLDRMLRLLACHSVVGCSIVSDKDGNVQRLYSLTPISKCYVRNEDGVSL 120
           EITTTNP+AALMLDRMLRLLACHSVVGCSI SDKDGNVQRLYSLTPISK YVRNEDGVSL
Sbjct: 61  EITTTNPDAALMLDRMLRLLACHSVVGCSIASDKDGNVQRLYSLTPISKYYVRNEDGVSL 120

Query: 121 GPMLSLIQDKVFLQSWFALLN 142
           GP LSLIQDKVFLQSW  L N
Sbjct: 121 GPTLSLIQDKVFLQSWSELKN 141

[Close]

BLAST of CSPI03G04010 vs. NCBI nr
Match: gi|659096296|ref|XP_008449020.1| (PREDICTED: caffeic acid 3-O-methyltransferase-like isoform X3 [Cucumis melo])

HSP 1 Score: 219.5 bits (558), Expect = 4.8e-54
Identity = 120/141 (85.11%), Postives = 124/141 (87.94%), Query Frame = 1


Query: 1   MASSDNDTAVEQQQHYAYAGHLVTLSVLPMTLQAVFELGVFEILAKAGDGANLSPAEIAA 60
           MASSDNDTAVEQQ +YA A  LVTLSVLPMTLQAVFELGVFEILAKAGD   LSPAEI A
Sbjct: 1   MASSDNDTAVEQQ-NYANAAELVTLSVLPMTLQAVFELGVFEILAKAGD-RELSPAEITA 60

Query: 61  EITTTNPNAALMLDRMLRLLACHSVVGCSIVSDKDGNVQRLYSLTPISKCYVRNEDGVSL 120
           EITTTNP AA M+DRMLRLLA HSVVGCSI SD+DGNVQR YSLTPISK YVRNEDGVSL
Sbjct: 61  EITTTNPYAASMIDRMLRLLASHSVVGCSIASDEDGNVQRFYSLTPISKYYVRNEDGVSL 120

Query: 121 GPMLSLIQDKVFLQSWFALLN 142
           GP+LSLIQDKVFLQSW  L N
Sbjct: 121 GPILSLIQDKVFLQSWSELKN 139

[Close]

BLAST of CSPI03G04010 vs. NCBI nr
Match: gi|659096292|ref|XP_008449018.1| (PREDICTED: caffeic acid 3-O-methyltransferase-like isoform X1 [Cucumis melo])

HSP 1 Score: 219.5 bits (558), Expect = 4.8e-54
Identity = 120/141 (85.11%), Postives = 124/141 (87.94%), Query Frame = 1


Query: 1   MASSDNDTAVEQQQHYAYAGHLVTLSVLPMTLQAVFELGVFEILAKAGDGANLSPAEIAA 60
           MASSDNDTAVEQQ +YA A  LVTLSVLPMTLQAVFELGVFEILAKAGD   LSPAEI A
Sbjct: 1   MASSDNDTAVEQQ-NYANAAELVTLSVLPMTLQAVFELGVFEILAKAGD-RELSPAEITA 60

Query: 61  EITTTNPNAALMLDRMLRLLACHSVVGCSIVSDKDGNVQRLYSLTPISKCYVRNEDGVSL 120
           EITTTNP AA M+DRMLRLLA HSVVGCSI SD+DGNVQR YSLTPISK YVRNEDGVSL
Sbjct: 61  EITTTNPYAASMIDRMLRLLASHSVVGCSIASDEDGNVQRFYSLTPISKYYVRNEDGVSL 120

Query: 121 GPMLSLIQDKVFLQSWFALLN 142
           GP+LSLIQDKVFLQSW  L N
Sbjct: 121 GPILSLIQDKVFLQSWSELKN 139

[Close]

BLAST of CSPI03G04010 vs. NCBI nr
Match: gi|449466440|ref|XP_004150934.1| (PREDICTED: caffeic acid 3-O-methyltransferase-like [Cucumis sativus])

HSP 1 Score: 204.1 bits (518), Expect = 2.1e-49
Identity = 103/139 (74.10%), Postives = 120/139 (86.33%), Query Frame = 1


Query: 3   SSDNDTAVEQQQHYAYAGHLVTLSVLPMTLQAVFELGVFEILAKAGDGANLSPAEIAAEI 62
           S D+DT +EQQ HYAYA  L TL+V+PMTLQA FELGVFEILAKAG+GA LS  EIAA+I
Sbjct: 14  SEDDDTVMEQQ-HYAYAMELATLAVVPMTLQAAFELGVFEILAKAGNGAELSSTEIAAKI 73

Query: 63  TTTNPNAALMLDRMLRLLACHSVVGCSIVSDKDGNVQRLYSLTPISKCYVRNEDGVSLGP 122
           TTTNP A+LM+DR+LRLLA H+VVGCS+ SD+DGNVQRLYSLTP+SK YVRNEDGVSLGP
Sbjct: 74  TTTNPEASLMIDRILRLLASHAVVGCSLASDEDGNVQRLYSLTPVSKYYVRNEDGVSLGP 133

Query: 123 MLSLIQDKVFLQSWFALLN 142
           +L+L+QDKV L +W  L N
Sbjct: 134 LLTLLQDKVLLHTWSELKN 151

The following BLAST results are available for this feature:

BLAST of CSPI03G04010 vs. Swiss-Prot


Match Name	E-value	Identity	Description
COMT1_CAPAN	3.3e-31	47.48	Caffeic acid 3-O-methyltransferase OS=Capsicum annuum GN=COMT PE=2 SV=2	[more]
COMT1_MEDSA	4.4e-31	49.24	Caffeic acid 3-O-methyltransferase OS=Medicago sativa PE=1 SV=1	[more]
COMT1_CLABR	9.7e-31	52.59	Caffeic acid 3-O-methyltransferase OS=Clarkia breweri GN=COMT PE=1 SV=1	[more]
COMT1_CAPCH	3.7e-30	46.76	Caffeic acid 3-O-methyltransferase OS=Capsicum chinense GN=COMT PE=2 SV=1	[more]
COMT1_POPTM	4.1e-29	48.48	Caffeic acid 3-O-methyltransferase 1 OS=Populus tremuloides GN=OMT1 PE=1 SV=1	[more]

BLAST of CSPI03G04010 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A061GSD8_THECC	1.0e-34	52.60	Caffeic acid 3-O-methyltransferase 1 OS=Theobroma cacao GN=TCM_040759 PE=3 SV=1	[more]
A0A061GUA5_THECC	1.7e-34	54.42	Caffeic acid 3-O-methyltransferase 1 isoform 2 OS=Theobroma cacao GN=TCM_040763 ...	[more]
A0A061GTC8_THECC	1.7e-34	54.42	Caffeic acid 3-O-methyltransferase 1 isoform 1 OS=Theobroma cacao GN=TCM_040763 ...	[more]
W9S415_9ROSA	1.5e-33	58.52	Caffeic acid 3-O-methyltransferase OS=Morus notabilis GN=L484_019621 PE=3 SV=1	[more]
W9SI12_9ROSA	1.9e-33	53.79	Caffeic acid 3-O-methyltransferase OS=Morus notabilis GN=L484_013036 PE=3 SV=1	[more]

BLAST of CSPI03G04010 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G54160.1	1.0e-22	40.27	O-methyltransferase 1	[more]
AT1G77520.1	2.2e-20	38.06	O-methyltransferase family protein	[more]
AT1G77530.1	1.4e-19	37.31	O-methyltransferase family protein	[more]
AT1G33030.1	3.2e-16	36.64	O-methyltransferase family protein	[more]
AT1G51990.1	9.4e-16	34.96	O-methyltransferase family protein	[more]

BLAST of CSPI03G04010 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778675776\|ref\|XP_004150935.2\|	6.9e-69	97.16	PREDICTED: caffeic acid 3-O-methyltransferase-like [Cucumis sativus]	[more]
gi\|778675782\|ref\|XP_004148264.2\|	1.9e-63	92.20	PREDICTED: caffeic acid 3-O-methyltransferase [Cucumis sativus]	[more]
gi\|659096296\|ref\|XP_008449020.1\|	4.8e-54	85.11	PREDICTED: caffeic acid 3-O-methyltransferase-like isoform X3 [Cucumis melo]	[more]
gi\|659096292\|ref\|XP_008449018.1\|	4.8e-54	85.11	PREDICTED: caffeic acid 3-O-methyltransferase-like isoform X1 [Cucumis melo]	[more]
gi\|449466440\|ref\|XP_004150934.1\|	2.1e-49	74.10	PREDICTED: caffeic acid 3-O-methyltransferase-like [Cucumis sativus]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR011991	Winged helix-turn-helix DNA-binding domain
IPR012967	Plant_MeTrfase_dimerisation

Vocabulary: **Molecular Function**
Term	Definition
GO:0046983	protein dimerization activity

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
biological_process	GO:0032259	methylation
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0008168	methyltransferase activity
molecular_function	GO:0046983	protein dimerization activity
molecular_function	GO:0008171	O-methyltransferase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G04010.1	CSPI03G04010.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR011991	Winged helix-turn-helix DNA-binding domain	GENE3D	G3DSA:1.10.10.10		coord: 26..139 score: 7.1
IPR011991	Winged helix-turn-helix DNA-binding domain	unknown	SSF46785	"Winged helix" DNA-binding domain	coord: 11..114 score: 5.44
IPR012967	Plant methyltransferase dimerisation	PFAM	PF08100	Dimerisation	coord: 30..81 score: 5.2
None	No IPR available	PANTHER	PTHR11746	O-METHYLTRANSFERASE	coord: 1..141 score: 6.4
None	No IPR available	PANTHER	PTHR11746:SF103	SUBFAMILY NOT NAMED	coord: 1..141 score: 6.4

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CSPI03G04010	MELO3C014094	Melon (DHL92) v3.5.1	cpimeB240
CSPI03G04010	Bhi11G000352	Wax gourd	cpiwgoB218
CSPI03G04010	CmaCh06G006830	Cucurbita maxima (Rimu)	cmacpiB838
CSPI03G04010	CmoCh06G006930	Cucurbita moschata (Rifu)	cmocpiB823

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CSPI03G04010	Cucurbita pepo (Zucchini)	cpecpiB216
CSPI03G04010	Cucurbita pepo (Zucchini)	cpecpiB277
CSPI03G04010	Cucurbita pepo (Zucchini)	cpecpiB852
CSPI03G04010	Bottle gourd (USVL1VR-Ls)	cpilsiB159
CSPI03G04010	Melon (DHL92) v3.6.1	cpimedB225
CSPI03G04010	Cucumber (Gy14) v2	cgybcpiB110
CSPI03G04010	Silver-seed gourd	carcpiB0004
CSPI03G04010	Silver-seed gourd	carcpiB0008
CSPI03G04010	Cucumber (Chinese Long) v3	cpicucB144
CSPI03G04010	Watermelon (97103) v2	cpiwmbB184
CSPI03G04010	Wax gourd	cpiwgoB324
CSPI03G04010	Wild cucumber (PI 183967)	cpicpiB141
CSPI03G04010	Cucumber (Gy14) v1	cgycpiB015
CSPI03G04010	Cucurbita maxima (Rimu)	cmacpiB333
CSPI03G04010	Cucurbita moschata (Rifu)	cmocpiB324
CSPI03G04010	Cucurbita moschata (Rifu)	cmocpiB542
CSPI03G04010	Cucumber (Chinese Long) v2	cpicuB120
CSPI03G04010	Watermelon (Charleston Gray)	cpiwcgB190
CSPI03G04010	Watermelon (Charleston Gray)	cpiwcgB194
CSPI03G04010	Watermelon (97103) v1	cpiwmB201