CSPI03G02050 (gene) Wild cucumber (PI 183967) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CSPI03G02050
Type	gene
Organism	Cucumis sativus (Wild cucumber (PI 183967))
Description	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein, putative
Location	Chr3 : 1507293 .. 1507958 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTTGTTAAAGAGTTCCAAATCAGTGATCGAAACATTGTAATCCATGAGCTTGACGACCTCTATGATTCCACAACTGGAAGAGTGCTCACCGGTTCCTGGGTCTGGAACTCGGCCTTCGTCCTAGCCAAGTGGATGGCAACTCAGTGCAACCTATTCGACTTCGACTTCCGGCAGAAGAACGTAATCGAGCTTGGCGCTGGGACTGGACTTCCTGGTTTGACAGCAGCTTTACTTGGTGCTAATCGTGTTCTACTTACCGATGTAGAGCCATTGCTTCCTGGGTTGTTGGAGAATGTAGATGCTAATGGAGTTGGCGATCGAGTGGAAGTAAGGGAACTCGTGTGGGGCTCGAACGACTTGGCAAGTCAAGCAAATGAGTTGGGGGAGTTTGACCTGATTTTGATGTCAGATTTGTTCTACGACTCGGAGGAAATGCCCCATCTAGCACAAGTACTAAAGATCATCTCTGGGACAGATACCAAAATATGGGCAGCATCGGAGATCCGACCATGGACTCTTGAATGCATTACAGAGTTGATAAATCAAGGATTCAAGATCACAGAGTCCCCAGTTCAACATGATGAATCTCATGAGGAAGACAACCATAAAATTTTGGACTCGTTTTCTATATTCCATCTCAGACAAGAAGAAAGAAACCTTTAA

mRNA sequence

ATGGTTGTTAAAGAGTTCCAAATCAGTGATCGAAACATTGTAATCCATGAGCTTGACGACCTCTATGATTCCACAACTGGAAGAGTGCTCACCGGTTCCTGGGTCTGGAACTCGGCCTTCGTCCTAGCCAAGTGGATGGCAACTCAGTGCAACCTATTCGACTTCGACTTCCGGCAGAAGAACGTAATCGAGCTTGGCGCTGGGACTGGACTTCCTGGTTTGACAGCAGCTTTACTTGGTGCTAATCGTGTTCTACTTACCGATGTAGAGCCATTGCTTCCTGGGTTGTTGGAGAATGTAGATGCTAATGGAGTTGGCGATCGAGTGGAAGTAAGGGAACTCGTGTGGGGCTCGAACGACTTGGCAAGTCAAGCAAATGAGTTGGGGGAGTTTGACCTGATTTTGATGTCAGATTTGTTCTACGACTCGGAGGAAATGCCCCATCTAGCACAAGTACTAAAGATCATCTCTGGGACAGATACCAAAATATGGGCAGCATCGGAGATCCGACCATGGACTCTTGAATGCATTACAGAGTTGATAAATCAAGGATTCAAGATCACAGAGTCCCCAGTTCAACATGATGAATCTCATGAGGAAGACAACCATAAAATTTTGGACTCGTTTTCTATATTCCATCTCAGACAAGAAGAAAGAAACCTTTAA

Coding sequence (CDS)

ATGGTTGTTAAAGAGTTCCAAATCAGTGATCGAAACATTGTAATCCATGAGCTTGACGACCTCTATGATTCCACAACTGGAAGAGTGCTCACCGGTTCCTGGGTCTGGAACTCGGCCTTCGTCCTAGCCAAGTGGATGGCAACTCAGTGCAACCTATTCGACTTCGACTTCCGGCAGAAGAACGTAATCGAGCTTGGCGCTGGGACTGGACTTCCTGGTTTGACAGCAGCTTTACTTGGTGCTAATCGTGTTCTACTTACCGATGTAGAGCCATTGCTTCCTGGGTTGTTGGAGAATGTAGATGCTAATGGAGTTGGCGATCGAGTGGAAGTAAGGGAACTCGTGTGGGGCTCGAACGACTTGGCAAGTCAAGCAAATGAGTTGGGGGAGTTTGACCTGATTTTGATGTCAGATTTGTTCTACGACTCGGAGGAAATGCCCCATCTAGCACAAGTACTAAAGATCATCTCTGGGACAGATACCAAAATATGGGCAGCATCGGAGATCCGACCATGGACTCTTGAATGCATTACAGAGTTGATAAATCAAGGATTCAAGATCACAGAGTCCCCAGTTCAACATGATGAATCTCATGAGGAAGACAACCATAAAATTTTGGACTCGTTTTCTATATTCCATCTCAGACAAGAAGAAAGAAACCTTTAA

Homology

[Close]

BLAST of CSPI03G02050 vs. Swiss-Prot
Match: MT21A_DANRE (Protein N-lysine methyltransferase METTL21A OS=Danio rerio GN=mettl21a PE=2 SV=1)

HSP 1 Score: 93.6 bits (231), Expect = 2.9e-18
Identity = 69/169 (40.83%), Postives = 89/169 (52.66%), Query Frame = 1


Query: 35  VWNSAFVLAKWMATQCNLFDFDFRQKNVIELGAGTGLPGLTAALLGANRVLLTDVEPLLP 94
           VW++A VL  ++     +   D + K VIELGAGTGL G+ AALLGAN V +TD EP L 
Sbjct: 46  VWDAAVVLCMFL----EMGKVDLKGKRVIELGAGTGLVGIVAALLGAN-VTITDREPALE 105

Query: 95  GLLENVDAN---GVGDRVEVRELVWGSN-DLASQANELGEFDLILMSDLFYDSEEMPHLA 154
            L  NV  N   G    V+V EL WG N DL  Q    G +DLIL +D+ Y  E  P L 
Sbjct: 106 FLTANVHENIPQGRQKAVQVSELTWGENLDLYPQ----GGYDLILGADIVYLEETFPALL 165

Query: 155 QVLKIISGTDTKIWAASEIRPWTLECITELINQGFKITESPVQHDESHE 200
           Q L+ +S  DT +  +  IR    E     + Q F + E  V +D   +
Sbjct: 166 QTLEHLSSGDTVVLLSCRIRYERDERFLTELRQRFSVQE--VHYDSQRD 203

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BLAST of CSPI03G02050 vs. Swiss-Prot
Match: MT21B_BOVIN (Protein-lysine methyltransferase METTL21B OS=Bos taurus GN=METTL21B PE=2 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 8.8e-15
Identity = 59/166 (35.54%), Postives = 87/166 (52.41%), Query Frame = 1


Query: 35  VWNSAFVLAKWMATQCNLFDFDFRQKNVIELGAGTGLPGLTAALLGANRVLLTDVEPLLP 94
           VW++A  L  +  +Q    + DFR K VIELGAGTG+ G+ AAL G + V +TD+  +L 
Sbjct: 56  VWDAALSLCNYFESQ----NVDFRGKKVIELGAGTGIVGILAALQGGD-VTITDLPLVLE 115

Query: 95  GLLENVDAN-GVGDRVEVRELVWGSNDLASQANELGEFDLILMSDLFYDSEEMPHLAQVL 154
            +  NV AN   G R +VR L WG      Q    G++DL+L +D+ Y     P L   L
Sbjct: 116 QIQGNVQANVPPGGRAQVRALSWG----IDQHVFPGDYDLVLGADIVYLEPTFPLLLGTL 175

Query: 155 KIISGTDTKIWAASEIRP--WTLECITELINQGFKITESPVQHDES 198
           + + G    I+ AS++R    T      L+ Q F++  +    DE+
Sbjct: 176 RHLCGPHGTIYLASKMREEHGTESFFQHLLPQHFQLELAQRDEDEN 212

[Close]

BLAST of CSPI03G02050 vs. Swiss-Prot
Match: MT21D_MOUSE (Protein-lysine methyltransferase METTL21D OS=Mus musculus GN=Vcpkmt PE=2 SV=2)

HSP 1 Score: 80.9 bits (198), Expect = 2.0e-14
Identity = 66/183 (36.07%), Postives = 96/183 (52.46%), Query Frame = 1


Query: 32  GSWVWNSAFVLAKWMATQCNLFD--FDFRQKNVIELGAGTGLPGLTAALLGANRVLLTDV 91
           G  VW++A VL+K++ T     D      +++V+ELG+GTG  GL AA LGA+ V++TD+
Sbjct: 39  GCVVWDAAIVLSKYLETPGFSGDGAHALSRRSVLELGSGTGAVGLMAATLGAD-VIVTDL 98

Query: 92  EPLLPGLLENVDANG--VGDRVEVRELVWGSNDLASQANELGEFDLILMSDLFYDSEEMP 151
           E L   L  N+D N   V   V+ + L WG +       +L   D ILM+D  Y  E + 
Sbjct: 99  EELQDLLKMNIDMNKHLVTGSVQAKVLKWGED-----IEDLMSPDYILMADCIYYEESLE 158

Query: 152 HLAQVLKIISGTDTKIWAASEIR-----PWTLECITELINQGFKITESPV-QHDESH-EE 204
            L + LK +SG++T I    E R     P   +   EL+   F   E P+ +HDE +  E
Sbjct: 159 PLLKTLKDLSGSETCIICCYEQRTMGKNPEIEKKYFELLQLDFDFEEIPLDKHDEEYRSE 215

[Close]

BLAST of CSPI03G02050 vs. Swiss-Prot
Match: MT21A_HUMAN (Protein N-lysine methyltransferase METTL21A OS=Homo sapiens GN=METTL21A PE=1 SV=2)

HSP 1 Score: 79.3 bits (194), Expect = 5.7e-14
Identity = 55/139 (39.57%), Postives = 78/139 (56.12%), Query Frame = 1


Query: 35  VWNSAFVLAKWMATQCNLFDFDFRQKNVIELGAGTGLPGLTAALLGANRVLLTDVEPLLP 94
           VW++A VL+ ++     +   + R ++ +ELGAGTGL G+ AALLGA+ V +TD +  L 
Sbjct: 46  VWDAAIVLSTYL----EMGAVELRGRSAVELGAGTGLVGIVAALLGAH-VTITDRKVALE 105

Query: 95  GLLENVDAN---GVGDRVEVRELVWGSNDLASQANELGEFDLILMSDLFYDSEEMPHLAQ 154
            L  NV AN    +  +  V+EL WG N L S +   GEFDLIL +D+ Y  E    L Q
Sbjct: 106 FLKSNVQANLPPHIQTKTVVKELTWGQN-LGSFSP--GEFDLILGADIIYLEETFTDLLQ 165

Query: 155 VLKIISGTDTKIWAASEIR 171
            L+ +    + I  A  IR
Sbjct: 166 TLEHLCSNHSVILLACRIR 176

[Close]

BLAST of CSPI03G02050 vs. Swiss-Prot
Match: MT21B_HUMAN (Protein-lysine methyltransferase METTL21B OS=Homo sapiens GN=METTL21B PE=1 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 2.2e-13
Identity = 57/166 (34.34%), Postives = 85/166 (51.20%), Query Frame = 1


Query: 35  VWNSAFVLAKWMATQCNLFDFDFRQKNVIELGAGTGLPGLTAALLGANRVLLTDVEPLLP 94
           VW++A  L  +  +Q    + DFR K VIELGAGTG+ G+ AAL G + V +TD+   L 
Sbjct: 56  VWDAALSLCNYFESQ----NVDFRGKKVIELGAGTGIVGILAALQGGD-VTITDLPLALE 115

Query: 95  GLLENVDAN-GVGDRVEVRELVWGSNDLASQANELGEFDLILMSDLFYDSEEMPHLAQVL 154
            +  NV AN   G + +VR L WG +     AN    +DL+L +D+ Y     P L   L
Sbjct: 116 QIQGNVQANVPAGGQAQVRALSWGIDHHVFPAN----YDLVLGADIVYLEPTFPLLLGTL 175

Query: 155 KIISGTDTKIWAASEIRP--WTLECITELINQGFKITESPVQHDES 198
           + +      I+ AS++R    T      L+ Q F++  +    DE+
Sbjct: 176 QHLCRPHGTIYLASKMRKEHGTESFFQHLLPQHFQLELAQRDEDEN 212

[Close]

BLAST of CSPI03G02050 vs. TrEMBL
Match: A0A0A0L6N4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G016950 PE=4 SV=1)

HSP 1 Score: 444.9 bits (1143), Expect = 5.8e-122
Identity = 218/221 (98.64%), Postives = 220/221 (99.55%), Query Frame = 1


Query: 1   MVVKEFQISDRNIVIHELDDLYDSTTGRVLTGSWVWNSAFVLAKWMATQCNLFDFDFRQK 60
           MVVKEFQISDRNIVIHELDDLYDSTTGRVLTGSWVWNSAFVLAKWMATQCNLFDFDFRQK
Sbjct: 1   MVVKEFQISDRNIVIHELDDLYDSTTGRVLTGSWVWNSAFVLAKWMATQCNLFDFDFRQK 60

Query: 61  NVIELGAGTGLPGLTAALLGANRVLLTDVEPLLPGLLENVDANGVGDRVEVRELVWGSND 120
           NVIELGAGTGLPGLTAALLGANRVLLTDVEPLLPGLLENVDANGVGDRVEVRELVWGSND
Sbjct: 61  NVIELGAGTGLPGLTAALLGANRVLLTDVEPLLPGLLENVDANGVGDRVEVRELVWGSND 120

Query: 121 LASQANELGEFDLILMSDLFYDSEEMPHLAQVLKIISGTDTKIWAASEIRPWTLECITEL 180
           L SQANELGEFDLILMSDLFY+SEEMPHLAQVLKIISGTDTKIWAASEIRPWT+ECITEL
Sbjct: 121 LPSQANELGEFDLILMSDLFYNSEEMPHLAQVLKIISGTDTKIWAASEIRPWTIECITEL 180

Query: 181 INQGFKITESPVQHDESHEEDNHKILDSFSIFHLRQEERNL 222
           INQGFKITESPVQHDESHEEDNHKILDSFSIFHLRQEERNL
Sbjct: 181 INQGFKITESPVQHDESHEEDNHKILDSFSIFHLRQEERNL 221

[Close]

BLAST of CSPI03G02050 vs. TrEMBL
Match: U5GUZ2_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0001s03120g PE=4 SV=1)

HSP 1 Score: 242.3 bits (617), Expect = 5.7e-61
Identity = 114/195 (58.46%), Postives = 150/195 (76.92%), Query Frame = 1


Query: 3   VKEFQISDRNIVIHELDDLYDSTTGRVLTGSWVWNSAFVLAKWMATQCNLFDFDFRQKNV 62
           V+E  I+ + + IHEL ++YDS TGR  TGSW+W+SA +L++W+AT      FD R K+V
Sbjct: 40  VREIDIAGQTLKIHELGEVYDSLTGRAFTGSWIWDSALLLSRWLATS----QFDLRDKSV 99

Query: 63  IELGAGTGLPGLTAALLGANRVLLTDVEPLLPGLLENVDANGVGDRVEVRELVWGSNDLA 122
           IELGAG GLPGLTAALLGA+RVLLTD+ PLLPGL++NV+AN + DRVEVRELVWGS +  
Sbjct: 100 IELGAGAGLPGLTAALLGASRVLLTDIAPLLPGLVKNVEANELEDRVEVRELVWGSEESL 159

Query: 123 SQANELGEFDLILMSDLFYDSEEMPHLAQVLKIISGTDTKIWAASEIRPWTLECITELIN 182
           S+  EL  FD++L+SD+F+D EEM  L + LK +SG  T++ AASE+R WT EC+ EL++
Sbjct: 160 SRIGELRRFDVVLLSDVFFDLEEMAALGRTLKKVSGNKTRVLAASEVRFWTGECLNELVS 219

Query: 183 QGFKITESPVQHDES 198
           QGFK+ E P+Q D S
Sbjct: 220 QGFKVVEVPIQEDGS 230

[Close]

BLAST of CSPI03G02050 vs. TrEMBL
Match: A0A067LC29_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_11865 PE=4 SV=1)

HSP 1 Score: 240.7 bits (613), Expect = 1.7e-60
Identity = 116/214 (54.21%), Postives = 156/214 (72.90%), Query Frame = 1


Query: 1   MVVKEFQISDRNIVIHELDDLYDSTTGRVLTGSWVWNSAFVLAKWMATQCNLFDFDFRQK 60
           M ++E +I+ + + IHELDDL D+ TGR  TGSW+W+SA VL++W+ T       DF+ K
Sbjct: 1   MGIREIEIAGQTLTIHELDDLCDTITGRPFTGSWLWDSALVLSQWLVTS----QLDFKGK 60

Query: 61  NVIELGAGTGLPGLTAALLGANRVLLTDVEPLLPGLLENVDANGVGDRVEVRELVWGSND 120
           +V+ELGAGTGLPGLTAA LGA+ V+LTD+ PLLPGLL+NV+ N + +RV VREL WGS +
Sbjct: 61  SVLELGAGTGLPGLTAARLGASLVVLTDIAPLLPGLLKNVEVNVLANRVMVRELAWGSEE 120

Query: 121 LASQANELGEFDLILMSDLFYDSEEMPHLAQVLKIISGTDTKIWAASEIRPWTLECITEL 180
           L SQ +ELGEFD+ILMSDLF+D EEM +L   LK +SG  +K+WAASE+R WT EC+ +L
Sbjct: 121 LLSQISELGEFDVILMSDLFFDPEEMSNLGNTLKRVSGGRSKVWAASEVRTWTGECLNQL 180

Query: 181 INQGFKITESPVQHDESHEEDNHKILDSFSIFHL 215
           ++QGF + E P Q     +       D F+++HL
Sbjct: 181 VSQGFGVVEFPSQLGGGEQ-------DVFAVYHL 203

[Close]

BLAST of CSPI03G02050 vs. TrEMBL
Match: V4TEN4_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10033731mg PE=4 SV=1)

HSP 1 Score: 228.8 bits (582), Expect = 6.5e-57
Identity = 116/196 (59.18%), Postives = 151/196 (77.04%), Query Frame = 1


Query: 1   MVVKEFQISDRNIVIHELDDLYDSTTGRVLTGSWVWNSAFVLAKWMATQCNLFDFDFRQK 60
           M  +E +I   +I+I ELD+L DS TGR LTG+W+W+SA +LA++M+T      FDF+ K
Sbjct: 1   MSTREIEIGSNSIIIQELDNLCDSVTGRPLTGAWLWDSALILAQFMSTH-----FDFQNK 60

Query: 61  NVIELGAGTGLPGLTAALLGANRVLLTDVEPLLPGLLENVDANGVGDRVEVRELVWGSND 120
           +V+ELGAG GLPGLTAA LGA RV+LTDV+PLLPGL+ NV+ANG+GDRVEVRELVWGS+D
Sbjct: 61  SVLELGAGAGLPGLTAARLGATRVVLTDVKPLLPGLINNVEANGLGDRVEVRELVWGSDD 120

Query: 121 LASQANELGEFDLILMSDLFYDSEEMPHLAQVLKIISGT--DTKIWAASEIRPWTLECIT 180
           L SQ +ELGEFD+++MSD+FYD EEM  L + LK + GT   T +WA SE+R  T +C+ 
Sbjct: 121 L-SQLSELGEFDMVIMSDVFYDPEEMVGLGKTLKRVCGTGRHTVVWAVSEVRTRTGDCLH 180

Query: 181 ELI-NQGFKITESPVQ 194
           ELI +QGF++ E   Q
Sbjct: 181 ELIMSQGFRVIELTCQ 190

[Close]

BLAST of CSPI03G02050 vs. TrEMBL
Match: A0A061G4K6_THECC (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein, putative OS=Theobroma cacao GN=TCM_015801 PE=4 SV=1)

HSP 1 Score: 227.6 bits (579), Expect = 1.5e-56
Identity = 115/193 (59.59%), Postives = 142/193 (73.58%), Query Frame = 1


Query: 1   MVVKEFQISDRNI----VIHELDDLYDSTTGRVLTGSWVWNSAFVLAKWMATQCNLFDFD 60
           M V+E Q++  N+    ++HELDD+ DS TGR LTGSW+W+SA VL+ WM T  N     
Sbjct: 1   MGVREIQVAAHNLNHSLIVHELDDVCDSVTGRPLTGSWLWDSALVLSHWMPTHLN----- 60

Query: 61  FRQKNVIELGAGTGLPGLTAALLGANRVLLTDVEPLLPGLLENVDANGVGDRVEVRELVW 120
           F  K VIELGAG GLPGLTAALLGA RVLLTDV+PLL GLL+NV+ANG   RV+VRELVW
Sbjct: 61  FHGKAVIELGAGAGLPGLTAALLGATRVLLTDVQPLLAGLLKNVEANGFEGRVDVRELVW 120

Query: 121 GSNDLASQANELGEFDLILMSDLFYDSEEMPHLAQVLKIISGTDTKIWAASEIRPWTLEC 180
           GS++  S   E   FD++LMSD+FYD EEM  L++ LK I    T+IWAASE+RPWT EC
Sbjct: 121 GSDESISGLTESSTFDVVLMSDVFYDPEEMVGLSRTLKRICEEGTEIWAASELRPWTGEC 180

Query: 181 ITELINQGFKITE 190
           + EL +QGF++ E
Sbjct: 181 LNELRDQGFQVVE 188

[Close]

BLAST of CSPI03G02050 vs. TAIR10
Match: AT5G44170.1 (AT5G44170.1 S-adenosyl-L-methionine-dependent methyltransferases superfamily protein)

HSP 1 Score: 80.5 bits (197), Expect = 1.4e-15
Identity = 54/193 (27.98%), Postives = 92/193 (47.67%), Query Frame = 1


Query: 27  GRVLTGSWVWNSAFVLAKWMATQCNLFD-------------FDFRQKNVIELGAGTGLPG 86
           G +  G+ VW  + +L+K+      L               FDFR++  IELG G G+ G
Sbjct: 25  GSMHVGTSVWPCSLILSKFAERWSTLDSSSSTTSPNPYAELFDFRRRRGIELGTGCGVAG 84

Query: 87  LTAALLGANRVLLTDVEPLLPGLLENVDAN--GVGDRVEVRELVWGSNDLASQANELGEF 146
           +   LLG   ++LTD+ P++P L  N+  N   +G  ++   + W + D  S       F
Sbjct: 85  MAFYLLGLTEIVLTDIAPVMPALKHNLKRNKTALGKSLKTSIVYWNNRDQISALKP--PF 144

Query: 147 DLILMSDLFYDSEEMPHLAQVLKIISGTDTKIWAASEIR-PWTLECITELINQGFKITES 201
           DL++ +D+ Y  E +  L   ++++   D  +    +IR P   +   EL +  FKI + 
Sbjct: 145 DLVIAADVVYIEESVGQLVTAMELLVADDGAVLLGYQIRSPEADKLFWELCDIVFKIEKV 204

[Close]

BLAST of CSPI03G02050 vs. TAIR10
Match: AT1G73320.1 (AT1G73320.1 S-adenosyl-L-methionine-dependent methyltransferases superfamily protein)

HSP 1 Score: 74.7 bits (182), Expect = 8.0e-14
Identity = 50/146 (34.25%), Postives = 74/146 (50.68%), Query Frame = 1


Query: 30  LTGSWVWNSAFVLAKWMATQCNLFDFDFRQKNVIELGAGTGLPGLTAALLGANRVLLTDV 89
           +TGS +W+S  VL K++    +        K ++ELG+G GL G  AALLG N V LTD+
Sbjct: 117 VTGSVMWDSGVVLGKFLEHSVDSKVLSLEGKKIVELGSGCGLVGCIAALLGGNAV-LTDL 176

Query: 90  EPLLPGLLENVDAN----GVGDRVEVRELVWGSNDLASQANELGEF-DLILMSDLFYDSE 149
              L  L +N+  N           V+ELVWG +      + +  F D +L SD+ Y  E
Sbjct: 177 PDRLRLLKKNIQTNLHRGNTRGSAIVQELVWGDD---PDPDLIEPFPDYVLGSDVIYSEE 236

Query: 150 EMPHLAQVLKIISGTDTKIWAASEIR 171
            + HL + L  +    T I+ + E+R
Sbjct: 237 AVHHLVKTLLQLCSDQTTIFLSGELR 258

[Close]

BLAST of CSPI03G02050 vs. TAIR10
Match: AT3G50850.1 (AT3G50850.1 Putative methyltransferase family protein)

HSP 1 Score: 63.2 bits (152), Expect = 2.4e-10
Identity = 44/126 (34.92%), Postives = 67/126 (53.17%), Query Frame = 1


Query: 61  NVIELGAGTGLPGL-TAALLGANRVLLTDVEPLLPGLLENVDANG-----VGDRVEVREL 120
           N++ELG+GTG+ G+  AA LGAN V +TD+  ++  L  N DAN       G +V V  L
Sbjct: 91  NIVELGSGTGIVGIAAAATLGAN-VTVTDLPNVIENLKFNADANAQVVAKFGGKVHVASL 150

Query: 121 VWGSNDLASQANELGE-FDLILMSDLFYDSEEMPHLAQVLK--IISGTDTKIWAASEIRP 178
            WG  D       LG+  DLIL SD+ Y       L + L+  ++ G+  +++  + ++ 
Sbjct: 151 RWGEID---DVESLGQNVDLILASDVVYHVHLYEPLLKTLRFLLLEGSSERVFLMAHLKR 210

[Close]

BLAST of CSPI03G02050 vs. TAIR10
Match: AT5G49560.1 (AT5G49560.1 Putative methyltransferase family protein)

HSP 1 Score: 60.1 bits (144), Expect = 2.0e-09
Identity = 45/149 (30.20%), Postives = 73/149 (48.99%), Query Frame = 1


Query: 61  NVIELGAGTGLPGLTAALLGANRVLLTDVEPLLPGLLENVDANG-----VGDRVEVRELV 120
           N++ELG+GTGL G+ AA+  +  V +TD+  +L  L  N +AN       G +V V  L 
Sbjct: 108 NILELGSGTGLVGIAAAITLSANVTVTDLPHVLDNLNFNAEANAEIVERFGGKVNVAPLR 167

Query: 121 WGSNDLASQANELGE-FDLILMSDLFYDSEEMPHLAQVLKIISGTDTK-IWAASEIRPWT 180
           WG    A     LG+  DLIL SD+ Y       L + L+++     + I+  + +R W 
Sbjct: 168 WGE---ADDVEVLGQNVDLILASDVVYHDHLYEPLLKTLRLMQLEGKRLIFLMAHLRRWK 227

Query: 181 LECITELINQGFKITESPVQHDESHEEDN 203
            E +     +  K+ +  V H +  +E +
Sbjct: 228 KESV--FFKKARKLFDVDVIHSDVPQESS 251

[Close]

BLAST of CSPI03G02050 vs. TAIR10
Match: AT4G35987.1 (AT4G35987.1 S-adenosyl-L-methionine-dependent methyltransferases superfamily protein)

HSP 1 Score: 58.9 bits (141), Expect = 4.5e-09
Identity = 51/159 (32.08%), Postives = 81/159 (50.94%), Query Frame = 1


Query: 3   VKEFQISDRNIVIHELDDLYDSTTGRVLTGSWVWNSAFVLAKWMATQCNLFDFDFRQKNV 62
           + +F+IS+R    + +D+     TG V      W S  VLA +  +Q       FR K V
Sbjct: 89  LNDFEISNR----YNIDN-----TGLVCQ----WPSEEVLAYFCKSQPE----RFRGKRV 148

Query: 63  IELGAGTGLPGLT-AALLGANRVLLTDVEPLLPGLLE-NVDANGV---GDRVEVRELVWG 122
           IELG+G GL GL  AA   A+ V+++D  P +   ++ N++ N +   G  V+  EL W 
Sbjct: 149 IELGSGYGLAGLVIAAATEASEVVISDGNPQVVNYIKRNIETNSMAFGGTSVKAMELHWN 208

Query: 123 SNDLASQANELGEFDLILMSDLFYDSEEMPHLAQVLKII 157
            + L+   N    FD+I+ SD  +  E    LA+ +K++
Sbjct: 209 QHQLSELTN---TFDIIVASDCTFFKEFHKDLARTIKML 227

[Close]

BLAST of CSPI03G02050 vs. NCBI nr
Match: gi|449464918|ref|XP_004150176.1| (PREDICTED: protein N-lysine methyltransferase METTL21A-like [Cucumis sativus])

HSP 1 Score: 444.9 bits (1143), Expect = 8.3e-122
Identity = 218/221 (98.64%), Postives = 220/221 (99.55%), Query Frame = 1


Query: 1   MVVKEFQISDRNIVIHELDDLYDSTTGRVLTGSWVWNSAFVLAKWMATQCNLFDFDFRQK 60
           MVVKEFQISDRNIVIHELDDLYDSTTGRVLTGSWVWNSAFVLAKWMATQCNLFDFDFRQK
Sbjct: 1   MVVKEFQISDRNIVIHELDDLYDSTTGRVLTGSWVWNSAFVLAKWMATQCNLFDFDFRQK 60

Query: 61  NVIELGAGTGLPGLTAALLGANRVLLTDVEPLLPGLLENVDANGVGDRVEVRELVWGSND 120
           NVIELGAGTGLPGLTAALLGANRVLLTDVEPLLPGLLENVDANGVGDRVEVRELVWGSND
Sbjct: 61  NVIELGAGTGLPGLTAALLGANRVLLTDVEPLLPGLLENVDANGVGDRVEVRELVWGSND 120

Query: 121 LASQANELGEFDLILMSDLFYDSEEMPHLAQVLKIISGTDTKIWAASEIRPWTLECITEL 180
           L SQANELGEFDLILMSDLFY+SEEMPHLAQVLKIISGTDTKIWAASEIRPWT+ECITEL
Sbjct: 121 LPSQANELGEFDLILMSDLFYNSEEMPHLAQVLKIISGTDTKIWAASEIRPWTIECITEL 180

Query: 181 INQGFKITESPVQHDESHEEDNHKILDSFSIFHLRQEERNL 222
           INQGFKITESPVQHDESHEEDNHKILDSFSIFHLRQEERNL
Sbjct: 181 INQGFKITESPVQHDESHEEDNHKILDSFSIFHLRQEERNL 221

[Close]

BLAST of CSPI03G02050 vs. NCBI nr
Match: gi|659095743|ref|XP_008448745.1| (PREDICTED: protein N-lysine methyltransferase METTL21A-like [Cucumis melo])

HSP 1 Score: 420.6 bits (1080), Expect = 1.7e-114
Identity = 207/222 (93.24%), Postives = 216/222 (97.30%), Query Frame = 1


Query: 1   MVVKEFQISDRNIVIHELDDLYDSTTGRVLTGSWVWNSAFVLAKWMATQCNLFDFDFRQK 60
           MVVKEFQISDRNIVIHELDDLYDSTTGRVLTGSWVWNSAFVLA+WMATQC LFDFD RQK
Sbjct: 1   MVVKEFQISDRNIVIHELDDLYDSTTGRVLTGSWVWNSAFVLAEWMATQCKLFDFDLRQK 60

Query: 61  NVIELGAGTGLPGLTAALLGANRVLLTDVEPLLPGLLENVDANGVGDRVEVRELVWGSND 120
           NVIELGAG GLPGLTAALLGANRV+LTDV+PLL GLLENVDAN +GDRVEVRELVWGSN+
Sbjct: 61  NVIELGAGAGLPGLTAALLGANRVVLTDVKPLLLGLLENVDANRLGDRVEVRELVWGSNE 120

Query: 121 LASQANELGEFDLILMSDLFYDSEEMPHLAQVLKIISGTDTKIWAASEIRPWTLECITEL 180
           LASQANELGEFDLILMSDLFYDSEEMPHLAQVLKIISGTD+KIWAASEIRPWT+ECITEL
Sbjct: 121 LASQANELGEFDLILMSDLFYDSEEMPHLAQVLKIISGTDSKIWAASEIRPWTIECITEL 180

Query: 181 INQGFKITESPVQH-DESHEEDNHKILDSFSIFHLRQEERNL 222
           INQGFKITESPVQH DESHEEDNHK+LDS+SIFHLRQEERNL
Sbjct: 181 INQGFKITESPVQHDDESHEEDNHKVLDSYSIFHLRQEERNL 222

[Close]

BLAST of CSPI03G02050 vs. NCBI nr
Match: gi|694387903|ref|XP_009369684.1| (PREDICTED: protein N-lysine methyltransferase METTL21A-like [Pyrus x bretschneideri])

HSP 1 Score: 252.3 bits (643), Expect = 7.9e-64
Identity = 126/214 (58.88%), Postives = 162/214 (75.70%), Query Frame = 1


Query: 1   MVVKEFQISDRNIVIHELDDLYDSTTGRVLTGSWVWNSAFVLAKWMATQCNLFDFDFRQK 60
           M  ++ Q++D+ +VIHELDD+ DSTTGR LTGSWVW+SA VL+ WMATQ     F F+ K
Sbjct: 1   MGTRQIQVADQTLVIHELDDVSDSTTGRALTGSWVWDSALVLSNWMATQ-GAVHFRFQDK 60

Query: 61  NVIELGAGTGLPGLTAALLGANRVLLTDVEPLLPGLLENVDANGVGDRVEVRELVWGSND 120
           +V+ELGAG GLPGLTAA LGA+RVLLTD+ PLLPGLL NV+ANG+GDRV+VRELVWGS++
Sbjct: 61  SVLELGAGAGLPGLTAARLGASRVLLTDMGPLLPGLLRNVEANGLGDRVQVRELVWGSDE 120

Query: 121 LASQANELGEFDLILMSDLFYDSEEMPHLAQVLKIISGTDTKIWAASEIRPWTLECITEL 180
             SQ +EL   DLILMSD+FY+ EEM  LA+ LK +    T +WA++E+RPWT EC++EL
Sbjct: 121 SPSQISEL---DLILMSDVFYNQEEMVGLAKTLKSVCSRGTTVWASTEVRPWTAECLSEL 180

Query: 181 INQGFKITESPVQHDESHEEDNHKILDSFSIFHL 215
           +++GF + E P Q  E  E      LD F+IF +
Sbjct: 181 VHEGFGLVELPSQMVEEMEG-----LDCFAIFQV 205

[Close]

BLAST of CSPI03G02050 vs. NCBI nr
Match: gi|566146939|ref|XP_006368473.1| (hypothetical protein POPTR_0001s03120g [Populus trichocarpa])

HSP 1 Score: 242.3 bits (617), Expect = 8.2e-61
Identity = 114/195 (58.46%), Postives = 150/195 (76.92%), Query Frame = 1


Query: 3   VKEFQISDRNIVIHELDDLYDSTTGRVLTGSWVWNSAFVLAKWMATQCNLFDFDFRQKNV 62
           V+E  I+ + + IHEL ++YDS TGR  TGSW+W+SA +L++W+AT      FD R K+V
Sbjct: 40  VREIDIAGQTLKIHELGEVYDSLTGRAFTGSWIWDSALLLSRWLATS----QFDLRDKSV 99

Query: 63  IELGAGTGLPGLTAALLGANRVLLTDVEPLLPGLLENVDANGVGDRVEVRELVWGSNDLA 122
           IELGAG GLPGLTAALLGA+RVLLTD+ PLLPGL++NV+AN + DRVEVRELVWGS +  
Sbjct: 100 IELGAGAGLPGLTAALLGASRVLLTDIAPLLPGLVKNVEANELEDRVEVRELVWGSEESL 159

Query: 123 SQANELGEFDLILMSDLFYDSEEMPHLAQVLKIISGTDTKIWAASEIRPWTLECITELIN 182
           S+  EL  FD++L+SD+F+D EEM  L + LK +SG  T++ AASE+R WT EC+ EL++
Sbjct: 160 SRIGELRRFDVVLLSDVFFDLEEMAALGRTLKKVSGNKTRVLAASEVRFWTGECLNELVS 219

Query: 183 QGFKITESPVQHDES 198
           QGFK+ E P+Q D S
Sbjct: 220 QGFKVVEVPIQEDGS 230

[Close]

BLAST of CSPI03G02050 vs. NCBI nr
Match: gi|802540284|ref|XP_012075608.1| (PREDICTED: protein N-lysine methyltransferase METTL21A-like [Jatropha curcas])

HSP 1 Score: 240.7 bits (613), Expect = 2.4e-60
Identity = 116/214 (54.21%), Postives = 156/214 (72.90%), Query Frame = 1


Query: 1   MVVKEFQISDRNIVIHELDDLYDSTTGRVLTGSWVWNSAFVLAKWMATQCNLFDFDFRQK 60
           M ++E +I+ + + IHELDDL D+ TGR  TGSW+W+SA VL++W+ T       DF+ K
Sbjct: 1   MGIREIEIAGQTLTIHELDDLCDTITGRPFTGSWLWDSALVLSQWLVTS----QLDFKGK 60

Query: 61  NVIELGAGTGLPGLTAALLGANRVLLTDVEPLLPGLLENVDANGVGDRVEVRELVWGSND 120
           +V+ELGAGTGLPGLTAA LGA+ V+LTD+ PLLPGLL+NV+ N + +RV VREL WGS +
Sbjct: 61  SVLELGAGTGLPGLTAARLGASLVVLTDIAPLLPGLLKNVEVNVLANRVMVRELAWGSEE 120

Query: 121 LASQANELGEFDLILMSDLFYDSEEMPHLAQVLKIISGTDTKIWAASEIRPWTLECITEL 180
           L SQ +ELGEFD+ILMSDLF+D EEM +L   LK +SG  +K+WAASE+R WT EC+ +L
Sbjct: 121 LLSQISELGEFDVILMSDLFFDPEEMSNLGNTLKRVSGGRSKVWAASEVRTWTGECLNQL 180

Query: 181 INQGFKITESPVQHDESHEEDNHKILDSFSIFHL 215
           ++QGF + E P Q     +       D F+++HL
Sbjct: 181 VSQGFGVVEFPSQLGGGEQ-------DVFAVYHL 203

The following BLAST results are available for this feature:

BLAST of CSPI03G02050 vs. Swiss-Prot


Match Name	E-value	Identity	Description
MT21A_DANRE	2.9e-18	40.83	Protein N-lysine methyltransferase METTL21A OS=Danio rerio GN=mettl21a PE=2 SV=1	[more]
MT21B_BOVIN	8.8e-15	35.54	Protein-lysine methyltransferase METTL21B OS=Bos taurus GN=METTL21B PE=2 SV=1	[more]
MT21D_MOUSE	2.0e-14	36.07	Protein-lysine methyltransferase METTL21D OS=Mus musculus GN=Vcpkmt PE=2 SV=2	[more]
MT21A_HUMAN	5.7e-14	39.57	Protein N-lysine methyltransferase METTL21A OS=Homo sapiens GN=METTL21A PE=1 SV=...	[more]
MT21B_HUMAN	2.2e-13	34.34	Protein-lysine methyltransferase METTL21B OS=Homo sapiens GN=METTL21B PE=1 SV=1	[more]

BLAST of CSPI03G02050 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L6N4_CUCSA	5.8e-122	98.64	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G016950 PE=4 SV=1	[more]
U5GUZ2_POPTR	5.7e-61	58.46	Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0001s03120g PE=4 SV=1	[more]
A0A067LC29_JATCU	1.7e-60	54.21	Uncharacterized protein OS=Jatropha curcas GN=JCGZ_11865 PE=4 SV=1	[more]
V4TEN4_9ROSI	6.5e-57	59.18	Uncharacterized protein OS=Citrus clementina GN=CICLE_v10033731mg PE=4 SV=1	[more]
A0A061G4K6_THECC	1.5e-56	59.59	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein, putati...	[more]

BLAST of CSPI03G02050 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G44170.1	1.4e-15	27.98	S-adenosyl-L-methionine-dependent methyltransferases superfamily pro...	[more]
AT1G73320.1	8.0e-14	34.25	S-adenosyl-L-methionine-dependent methyltransferases superfamily pro...	[more]
AT3G50850.1	2.4e-10	34.92	Putative methyltransferase family protein	[more]
AT5G49560.1	2.0e-09	30.20	Putative methyltransferase family protein	[more]
AT4G35987.1	4.5e-09	32.08	S-adenosyl-L-methionine-dependent methyltransferases superfamily pro...	[more]

BLAST of CSPI03G02050 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449464918\|ref\|XP_004150176.1\|	8.3e-122	98.64	PREDICTED: protein N-lysine methyltransferase METTL21A-like [Cucumis sativus]	[more]
gi\|659095743\|ref\|XP_008448745.1\|	1.7e-114	93.24	PREDICTED: protein N-lysine methyltransferase METTL21A-like [Cucumis melo]	[more]
gi\|694387903\|ref\|XP_009369684.1\|	7.9e-64	58.88	PREDICTED: protein N-lysine methyltransferase METTL21A-like [Pyrus x bretschneid...	[more]
gi\|566146939\|ref\|XP_006368473.1\|	8.2e-61	58.46	hypothetical protein POPTR_0001s03120g [Populus trichocarpa]	[more]
gi\|802540284\|ref\|XP_012075608.1\|	2.4e-60	54.21	PREDICTED: protein N-lysine methyltransferase METTL21A-like [Jatropha curcas]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR019410	Methyltransf_16

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
biological_process	GO:0032259	methylation
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003674	molecular_function
molecular_function	GO:0008168	methyltransferase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G02050.1	CSPI03G02050.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR019410	Lysine methyltransferase	PFAM	PF10294	Methyltransf_16	coord: 31..170 score: 4.5
None	No IPR available	PANTHER	PTHR14614	HEPATOCELLULAR CARCINOMA-ASSOCIATED ANTIGEN	coord: 3..219 score: 2.1

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CSPI03G02050	Cla008773	Watermelon (97103) v1	cpiwmB200
CSPI03G02050	Csa3G016950	Cucumber (Chinese Long) v2	cpicuB120
CSPI03G02050	MELO3C013880	Melon (DHL92) v3.5.1	cpimeB240
CSPI03G02050	ClCG10G001950	Watermelon (Charleston Gray)	cpiwcgB190
CSPI03G02050	MELO3C013880.2	Melon (DHL92) v3.6.1	cpimedB225
CSPI03G02050	Cla97C10G186590	Watermelon (97103) v2	cpiwmbB184
CSPI03G02050	CmaCh16G010880	Cucurbita maxima (Rimu)	cmacpiB325
CSPI03G02050	CmoCh16G011380	Cucurbita moschata (Rifu)	cmocpiB316
CSPI03G02050	Cp4.1LG14g08970	Cucurbita pepo (Zucchini)	cpecpiB207
CSPI03G02050	CsGy3G001930	Cucumber (Gy14) v2	cgybcpiB110
CSPI03G02050	Carg23234	Silver-seed gourd	carcpiB0742

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CSPI03G02050	Wax gourd	cpiwgoB239
CSPI03G02050	Wild cucumber (PI 183967)	cpicpiB100
CSPI03G02050	Wild cucumber (PI 183967)	cpicpiB103
CSPI03G02050	Cucumber (Gy14) v1	cgycpiB474
CSPI03G02050	Cucumber (Gy14) v1	cgycpiB484
CSPI03G02050	Cucurbita maxima (Rimu)	cmacpiB262
CSPI03G02050	Cucurbita maxima (Rimu)	cmacpiB608
CSPI03G02050	Cucurbita maxima (Rimu)	cmacpiB838
CSPI03G02050	Cucurbita moschata (Rifu)	cmocpiB597
CSPI03G02050	Cucurbita moschata (Rifu)	cmocpiB823
CSPI03G02050	Cucumber (Chinese Long) v2	cpicuB122
CSPI03G02050	Cucumber (Chinese Long) v2	cpicuB125
CSPI03G02050	Melon (DHL92) v3.5.1	cpimeB218
CSPI03G02050	Melon (DHL92) v3.5.1	cpimeB238
CSPI03G02050	Watermelon (Charleston Gray)	cpiwcgB180
CSPI03G02050	Watermelon (Charleston Gray)	cpiwcgB235
CSPI03G02050	Watermelon (97103) v1	cpiwmB184
CSPI03G02050	Watermelon (97103) v1	cpiwmB240
CSPI03G02050	Cucurbita pepo (Zucchini)	cpecpiB010
CSPI03G02050	Cucurbita pepo (Zucchini)	cpecpiB720
CSPI03G02050	Bottle gourd (USVL1VR-Ls)	cpilsiB218
CSPI03G02050	Bottle gourd (USVL1VR-Ls)	cpilsiB227
CSPI03G02050	Melon (DHL92) v3.6.1	cpimedB213
CSPI03G02050	Cucumber (Gy14) v2	cgybcpiB113
CSPI03G02050	Silver-seed gourd	carcpiB0597
CSPI03G02050	Silver-seed gourd	carcpiB0818
CSPI03G02050	Cucumber (Chinese Long) v3	cpicucB144
CSPI03G02050	Cucumber (Chinese Long) v3	cpicucB146
CSPI03G02050	Watermelon (97103) v2	cpiwmbB261