CSPI03G02020 (gene) Wild cucumber (PI 183967) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CSPI03G02020
Type	gene
Organism	Cucumis sativus (Wild cucumber (PI 183967))
Description	Haloacid dehalogenase-like hydrolase family protein
Location	Chr3 : 1486097 .. 1487875 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAAGGAAAGATCGGAGCAAAGGGCTCTAATTTAAGTGTAAGTAGTTGTAAGTAAACATATGTTGATATTAATTTGCAGATTGGTTTCAATGGTGGGATTCCAATTGACGAAGAGTTTTACATTAAGAATATTGCTGGCAAGAACAATGACGGTATTATCCGAACCCTATTCCCGAATGATGTCGAACAAGGTGCAGAATATATGAATGAGAAGGAGGCTATGTTTAGGAGGTAATAAAATATTTATCTTTATATACTTTCGATAATATATCATTATAAGTTTTGATTGAGACAACATATAACTAATGAATTTAACGTAGCCATCTAATTTTTTCCAAACTTACCTGAGATCATTACTGATCTATCTTAACTTTCCAAAAAAATCATAAAATAAAATAGTTCTCATATGGTCAGTCACAGATATCATTGATCTTCTTATTGTTCACCAGCTTGACTTTCCACTTACTTTTTTTTTTTCTTCACGATTCATGCATTCGAATGTCAATTATAAACTCAAATAATAATAATAATAAGGAAAATTTTCAAATGTAGTAAATTTTACAAAATATTTATACTGTGAATATAACAAAATCACTAAAAAGATCAAATTTATTACACTTGATTATGTTTGTATGAAGGGTAAATATTTTGTGAAATGTTTTATTTTTTATAATTTTTATAATATTTATCATTATTATTTCTTTTTCTTTCCCGATATATATAATACAAATACACATATATTTTTATTAGCATTCTCTTTGCTCAATTCCTTTATCTCTTCTCCAATCAACGTTATTCATCTCTCACTCGTTAACTCATCTTATTTTTCAATAGTTACAACTATATTGTAGGGACATTTTTAAAAATAGAATAATAAATTAAAATATTTACAAGCTATAACAAAATTTTGGATTCTATCACAAATAGTGTTTTATCACTATAGCATATCAATAACTATTACAGATAGAATTTGCTATAGGTGTTGTAAATATCTTGTAAGATCTACTATTTTTAAAAATTCTCCTATATTATATTCTCTATAATTAATTTTCTTTTCCATATTAACTATTTATACAAAACCAAATAATTAATATCATATTCCACCTAAACAAACTTTTCCTCTCAAAATCCCAAATCAACTCAAACTCTCTAACTCTTTTAATTAACCAAATCTTCTACTTTTAATATTTGATAAATAATTTAAGATACAAATTTGGTAAAAATTTGAGATGTTTCACTTAATGTAACAGATTAGTTGTTGAACAATTAAAGCCGGTGAGTGGCTTGTATAAAGTGAAGAAATGGGTGGAAGATCACAGATTGAAATGTGCTGCAGTTACGAACTCCTCTCGAATTAATGCCGAGCTCATGATCTCGATGCTCGGTCTTACGGATTTTTTCGAGACTATTATTATAGGTGATGAATGTGAGCACCCCAAACCACATCCAGAACCATATTTAAAGGCTTTGGAAGCACTCCAAGTATCAAAGGATCACACTTTCATTTTTGAGGTCAGTCATATGAAATTTTCTTTTCTCCCCATTGTTTTGTTCGAAATTTGAAAACTTGAATGCTTTGCTTTGCTAGGATTCTCTAACAGGGATCAAAGCTGGAGTTGAAGCCGATATGCCTGTTGTCGGTATATCAATGAGAAATTCTGAGCAGTTGTTGATGCAAGCAAAGCCAGCCCTCCTCGTAAAAGACTACAACGATTTGAAATTGTGGTTAGCTTTGGACGAACTAGTTGTGAGAGGAGATACCATTGCAAATGCATGA

mRNA sequence

ATGAAAGGAAAGATCGGAGCAAAGGGCTCTAATTTAAGTATTGGTTTCAATGGTGGGATTCCAATTGACGAAGAGTTTTACATTAAGAATATTGCTGGCAAGAACAATGACGGTATTATCCGAACCCTATTCCCGAATGATGTCGAACAAGGTGCAGAATATATGAATGAGAAGGAGGCTATGTTTAGGAGATTAGTTGTTGAACAATTAAAGCCGGTGAGTGGCTTGTATAAAGTGAAGAAATGGGTGGAAGATCACAGATTGAAATGTGCTGCAGTTACGAACTCCTCTCGAATTAATGCCGAGCTCATGATCTCGATGCTCGGTCTTACGGATTTTTTCGAGACTATTATTATAGGTGATGAATGTGAGCACCCCAAACCACATCCAGAACCATATTTAAAGGCTTTGGAAGCACTCCAAGTATCAAAGGATCACACTTTCATTTTTGAGGATTCTCTAACAGGGATCAAAGCTGGAGTTGAAGCCGATATGCCTGTTGTCGGTATATCAATGAGAAATTCTGAGCAGTTGTTGATGCAAGCAAAGCCAGCCCTCCTCGTAAAAGACTACAACGATTTGAAATTGTGGTTAGCTTTGGACGAACTAGTTGTGAGAGGAGATACCATTGCAAATGCATGA

Coding sequence (CDS)

ATGAAAGGAAAGATCGGAGCAAAGGGCTCTAATTTAAGTATTGGTTTCAATGGTGGGATTCCAATTGACGAAGAGTTTTACATTAAGAATATTGCTGGCAAGAACAATGACGGTATTATCCGAACCCTATTCCCGAATGATGTCGAACAAGGTGCAGAATATATGAATGAGAAGGAGGCTATGTTTAGGAGATTAGTTGTTGAACAATTAAAGCCGGTGAGTGGCTTGTATAAAGTGAAGAAATGGGTGGAAGATCACAGATTGAAATGTGCTGCAGTTACGAACTCCTCTCGAATTAATGCCGAGCTCATGATCTCGATGCTCGGTCTTACGGATTTTTTCGAGACTATTATTATAGGTGATGAATGTGAGCACCCCAAACCACATCCAGAACCATATTTAAAGGCTTTGGAAGCACTCCAAGTATCAAAGGATCACACTTTCATTTTTGAGGATTCTCTAACAGGGATCAAAGCTGGAGTTGAAGCCGATATGCCTGTTGTCGGTATATCAATGAGAAATTCTGAGCAGTTGTTGATGCAAGCAAAGCCAGCCCTCCTCGTAAAAGACTACAACGATTTGAAATTGTGGTTAGCTTTGGACGAACTAGTTGTGAGAGGAGATACCATTGCAAATGCATGA

Homology

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BLAST of CSPI03G02020 vs. Swiss-Prot
Match: SGGP_ARATH (Haloacid dehalogenase-like hydrolase domain-containing protein Sgpp OS=Arabidopsis thaliana GN=SGPP PE=1 SV=2)

HSP 1 Score: 253.4 bits (646), Expect = 2.1e-66
Identity = 116/190 (61.05%), Postives = 152/190 (80.00%), Query Frame = 1


Query: 14  IGFNGGIPIDEEFYIKNIAGKNNDGIIRTLFPNDVEQGAEYMNEKEAMFRRLVVEQLKPV 73
           IGFN G+PIDE+F+++NIAGK+N  I   LFP+DV +G ++ +EKEA++R++V E++KP+
Sbjct: 51  IGFNNGVPIDEKFFVENIAGKHNSEIALLLFPDDVSRGLKFCDEKEALYRKIVAEKIKPL 110

Query: 74  SGLYKVKKWVEDHRLKCAAVTNSSRINAELMISMLGLTDFFETIIIGDECEHPKPHPEPY 133
            GL K+ KW+ED  LK AAVTN+ + NAELMIS LGLTDFF+ +I+G ECE PKPHP PY
Sbjct: 111 DGLIKLTKWIEDRGLKRAAVTNAPKENAELMISKLGLTDFFQAVILGSECEFPKPHPGPY 170

Query: 134 LKALEALQVSKDHTFIFEDSLTGIKAGVEADMPVVGISMRNSEQLLMQAKPALLVKDYND 193
           LKALE L VSK+HT +FEDS++GIKAGV A MPV+G++  N   LLMQAKPA L+++Y D
Sbjct: 171 LKALEVLNVSKEHTLVFEDSISGIKAGVAAGMPVIGLTTGNPASLLMQAKPAFLIENYAD 230

Query: 194 LKLWLALDEL 204
            KLW  L+EL
Sbjct: 231 PKLWAVLEEL 240

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BLAST of CSPI03G02020 vs. Swiss-Prot
Match: PPAX_BACHD (Pyrophosphatase PpaX OS=Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) GN=ppaX PE=3 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 2.0e-11
Identity = 50/157 (31.85%), Postives = 77/157 (49.04%), Query Frame = 1


Query: 41  RTLFPNDVEQG-AEYMNEKEAMFRRLVVEQLKPVSGLYKVKKWVEDHRLKCAAVTNSSRI 100
           + L P  VE+  A Y     A   +LV    +P  G+Y+  K + +   K A VT   R 
Sbjct: 55  KRLDPERVEEMVATYRKHNHAHHDKLV----EPYEGVYETVKTLHEQGFKLAIVTTKIRE 114

Query: 101 NAELMISMLGLTDFFETIIIGDECEHPKPHPEPYLKALEALQVSKDHTFIFEDSLTGIKA 160
            A   + + GL +FF+ I+  D+ E+ KP+PEP  KA+ AL   K+ T +  D+   I  
Sbjct: 115 TAMKGLKLFGLDEFFDVIVALDDVENVKPNPEPLEKAMNALGAKKEETIMVGDNSHDILG 174

Query: 161 GVEADM--PVVGISMRNSEQLLMQAKPALLVKDYNDL 195
           G  A +   VVG ++R  E  + Q  P  +++   DL
Sbjct: 175 GKNAGVKTAVVGYAIR-GEDYVRQFDPDYVLRSMPDL 206

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BLAST of CSPI03G02020 vs. Swiss-Prot
Match: GPH_NITOC (Phosphoglycolate phosphatase OS=Nitrosococcus oceani (strain ATCC 19707 / NCIMB 11848) GN=Noc_2493 PE=3 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 1.1e-09
Identity = 39/159 (24.53%), Postives = 73/159 (45.91%), Query Frame = 1


Query: 19  GIPIDEEFYIKNIAGKNNDGIIRTLFPNDVEQGAE--YMNEKEAMFRRLVVEQLKPVSGL 78
           G+P+  E  ++   G   + +I+    +++E   E     + E +F  L  +     S L
Sbjct: 35  GLPLRGETKVRQWVGNGVERLIKRALVDNMEGEPEEDLYQKAETIFLALYADNTSKRSHL 94

Query: 79  Y----KVKKWVEDHRLKCAAVTNSSRINAELMISMLGLTDFFETIIIGDECEHPKPHPEP 138
           Y    +   W++    +   VTN +      +++ LG+ D+FE +I GD     KPHP P
Sbjct: 95  YPGVNEGLAWLKSQGYRVGCVTNKAAQFTYPLLTELGIIDYFEIVISGDTLPEKKPHPAP 154

Query: 139 YLKALEALQVSKDHTFIFEDSLTGIKAGVEADMPVVGIS 172
            L A     ++ +   +  DS++ +KA   A+  +V +S
Sbjct: 155 LLHAASHFGIAPEKALMIGDSISDVKAARAANFQIVCLS 193

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BLAST of CSPI03G02020 vs. Swiss-Prot
Match: PPAX_BACWK (Pyrophosphatase PpaX OS=Bacillus weihenstephanensis (strain KBAB4) GN=ppaX PE=3 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 1.2e-08
Identity = 36/128 (28.12%), Postives = 61/128 (47.66%), Query Frame = 1


Query: 68  EQLKPVSGLYKVKKWVEDHRLKCAAVTNSSRINAELMISMLGLTDFFETIIIGDECEHPK 127
           E ++    +Y+  + ++    K   VT  +R   E+ + +  L  FF+ ++  D+ EH K
Sbjct: 79  ELVEEYETVYETVQELKKQGYKIGIVTTKARQTVEMGLKLSKLDQFFDVVVTIDDVEHVK 138

Query: 128 PHPEPYLKALEALQVSKDHTFIFEDSLTGIKAGVEADMPVVGISMRNSEQLLMQA-KPAL 187
           PHPEP  KALE L    + T +  D+   I  G  A    V +S     +  ++A KP  
Sbjct: 139 PHPEPLQKALELLDAKPEETLMVGDNHHDIVGGQNAGTKTVAVSWTLKGRAYLEAYKPDY 198

Query: 188 LVKDYNDL 195
           ++   +DL
Sbjct: 199 VLDKMSDL 206

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BLAST of CSPI03G02020 vs. Swiss-Prot
Match: PPAX_BACC2 (Pyrophosphatase PpaX OS=Bacillus cereus (strain G9842) GN=ppaX PE=3 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 3.5e-08
Identity = 34/128 (26.56%), Postives = 60/128 (46.88%), Query Frame = 1


Query: 68  EQLKPVSGLYKVKKWVEDHRLKCAAVTNSSRINAELMISMLGLTDFFETIIIGDECEHPK 127
           E ++    +Y+  + ++    K   VT  +R   E+ + +  L +FF+ ++  D+ EH K
Sbjct: 79  ELVEEYETVYETVRELKKQGYKVGIVTTKARQTVEMGLQLSKLDEFFDVVVTIDDVEHVK 138

Query: 128 PHPEPYLKALEALQVSKDHTFIFEDSLTGIKAGVEADMPVVGISMRNSEQLLMQA-KPAL 187
           PHPEP  KALE L    +   +  D+   I  G  A      +S     +  ++A KP  
Sbjct: 139 PHPEPLQKALELLDAKPEEALMVGDNHHDIVGGQNAGTKTAAVSWTLKGRAYLEAYKPDF 198

Query: 188 LVKDYNDL 195
           ++   +DL
Sbjct: 199 MLDKMSDL 206

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BLAST of CSPI03G02020 vs. TrEMBL
Match: A0A0A0L202_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G016920 PE=4 SV=1)

HSP 1 Score: 404.4 bits (1038), Expect = 8.3e-110
Identity = 200/200 (100.00%), Postives = 200/200 (100.00%), Query Frame = 1


Query: 14  IGFNGGIPIDEEFYIKNIAGKNNDGIIRTLFPNDVEQGAEYMNEKEAMFRRLVVEQLKPV 73
           IGFNGGIPIDEEFYIKNIAGKNNDGIIRTLFPNDVEQGAEYMNEKEAMFRRLVVEQLKPV
Sbjct: 51  IGFNGGIPIDEEFYIKNIAGKNNDGIIRTLFPNDVEQGAEYMNEKEAMFRRLVVEQLKPV 110

Query: 74  SGLYKVKKWVEDHRLKCAAVTNSSRINAELMISMLGLTDFFETIIIGDECEHPKPHPEPY 133
           SGLYKVKKWVEDHRLKCAAVTNSSRINAELMISMLGLTDFFETIIIGDECEHPKPHPEPY
Sbjct: 111 SGLYKVKKWVEDHRLKCAAVTNSSRINAELMISMLGLTDFFETIIIGDECEHPKPHPEPY 170

Query: 134 LKALEALQVSKDHTFIFEDSLTGIKAGVEADMPVVGISMRNSEQLLMQAKPALLVKDYND 193
           LKALEALQVSKDHTFIFEDSLTGIKAGVEADMPVVGISMRNSEQLLMQAKPALLVKDYND
Sbjct: 171 LKALEALQVSKDHTFIFEDSLTGIKAGVEADMPVVGISMRNSEQLLMQAKPALLVKDYND 230

Query: 194 LKLWLALDELVVRGDTIANA 214
           LKLWLALDELVVRGDTIANA
Sbjct: 231 LKLWLALDELVVRGDTIANA 250

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BLAST of CSPI03G02020 vs. TrEMBL
Match: A0A0A0L429_CUCSA (2-deoxyglucose-6-phosphate phosphatase OS=Cucumis sativus GN=Csa_3G016930 PE=4 SV=1)

HSP 1 Score: 313.9 bits (803), Expect = 1.5e-82
Identity = 152/200 (76.00%), Postives = 172/200 (86.00%), Query Frame = 1


Query: 14  IGFNGGIPIDEEFYIKNIAGKNNDGIIRTLFPNDVEQGAEYMNEKEAMFRRLVVEQLKPV 73
           IGFNGG+PIDEEF+IKN+AGK+ND I R LFP+D E+G ++ +EKEAMFRRLV EQLKPV
Sbjct: 51  IGFNGGVPIDEEFFIKNVAGKHNDDIARALFPDDFERGLKFCDEKEAMFRRLVTEQLKPV 110

Query: 74  SGLYKVKKWVEDHRLKCAAVTNSSRINAELMISMLGLTDFFETIIIGDECEHPKPHPEPY 133
            GLYKVKKW+ED  LK AAVTN+ R NAELMISMLGL DFFE IIIG EC+H KPHPEPY
Sbjct: 111 DGLYKVKKWIEDRGLKRAAVTNAPRPNAELMISMLGLKDFFEAIIIGGECKHAKPHPEPY 170

Query: 134 LKALEALQVSKDHTFIFEDSLTGIKAGVEADMPVVGISMRNSEQLLMQAKPALLVKDYND 193
           LKALE L+VSK+HTFIFEDS +GIKAGV A+MPVVGI+ RN EQLLMQAKP L+VKDY+D
Sbjct: 171 LKALEVLKVSKEHTFIFEDSASGIKAGVAAEMPVVGIATRNPEQLLMQAKPTLVVKDYDD 230

Query: 194 LKLWLALDELVVRGDTIANA 214
           LKLW ALDEL  RG T+ NA
Sbjct: 231 LKLWAALDELDKRGGTVKNA 250

[Close]

BLAST of CSPI03G02020 vs. TrEMBL
Match: A0A151TJ16_CAJCA (Phosphorylated carbohydrates phosphatase TM-1254 (Fragment) OS=Cajanus cajan GN=KK1_013342 PE=4 SV=1)

HSP 1 Score: 286.6 bits (732), Expect = 2.5e-74
Identity = 133/192 (69.27%), Postives = 164/192 (85.42%), Query Frame = 1


Query: 12  LSIGFNGGIPIDEEFYIKNIAGKNNDGIIRTLFPNDVEQGAEYMNEKEAMFRRLVVEQLK 71
           L IGFNGG+PIDEEF+I+ +AGK+ND I   LFP D+E+G +++++KEAMFRRL  EQLK
Sbjct: 37  LEIGFNGGVPIDEEFFIETVAGKHNDDIALALFPGDLERGLKFVDDKEAMFRRLAAEQLK 96

Query: 72  PVSGLYKVKKWVEDHRLKCAAVTNSSRINAELMISMLGLTDFFETIIIGDECEHPKPHPE 131
           P++GL KVKKW+E+H LK AAVTN+ R NAELMIS+LGL+DFFE IIIG ECEH KPHP+
Sbjct: 97  PLNGLDKVKKWIENHGLKRAAVTNAPRANAELMISLLGLSDFFEAIIIGGECEHAKPHPD 156

Query: 132 PYLKALEALQVSKDHTFIFEDSLTGIKAGVEADMPVVGISMRNSEQLLMQAKPALLVKDY 191
           PYLK LEAL+ SKDHTF+FEDS++GIKAGV A MPV+G++ RN E LLM+AKPA L+KDY
Sbjct: 157 PYLKGLEALKASKDHTFVFEDSVSGIKAGVAAGMPVIGLATRNPESLLMEAKPAFLIKDY 216

Query: 192 NDLKLWLALDEL 204
           +D KLW AL+EL
Sbjct: 217 DDPKLWAALEEL 228

[Close]

BLAST of CSPI03G02020 vs. TrEMBL
Match: W9S5E8_9ROSA (Phosphorylated carbohydrates phosphatase OS=Morus notabilis GN=L484_023341 PE=4 SV=1)

HSP 1 Score: 280.4 bits (716), Expect = 1.8e-72
Identity = 133/190 (70.00%), Postives = 160/190 (84.21%), Query Frame = 1


Query: 14  IGFNGGIPIDEEFYIKNIAGKNNDGIIRTLFPNDVEQGAEYMNEKEAMFRRLVVEQLKPV 73
           IGFNGG+PIDE+F+I+NIAGK+ND I R LFP+D ++G ++ ++KEAMFRRL  EQLKP+
Sbjct: 51  IGFNGGVPIDEDFFIENIAGKHNDDIARALFPDDFQRGLKFTDDKEAMFRRLAAEQLKPL 110

Query: 74  SGLYKVKKWVEDHRLKCAAVTNSSRINAELMISMLGLTDFFETIIIGDECEHPKPHPEPY 133
            GLYK+KKWVED  LK AAVTN+ R NAELMIS LGL+DFF+ +IIG EC+H KPHP+PY
Sbjct: 111 DGLYKLKKWVEDRGLKRAAVTNAPRPNAELMISRLGLSDFFDAVIIGSECDHAKPHPDPY 170

Query: 134 LKALEALQVSKDHTFIFEDSLTGIKAGVEADMPVVGISMRNSEQLLMQAKPALLVKDYND 193
           LKAL+ L+ SKDHTFIFEDS++GIKAGV A M VVGI+ RN E LLMQAKPA L+KDY D
Sbjct: 171 LKALDILKASKDHTFIFEDSVSGIKAGVAAGMQVVGIATRNPEHLLMQAKPAFLIKDYAD 230

Query: 194 LKLWLALDEL 204
            KLW AL+EL
Sbjct: 231 PKLWEALEEL 240

[Close]

BLAST of CSPI03G02020 vs. TrEMBL
Match: I1J9M8_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_01G202000 PE=4 SV=1)

HSP 1 Score: 280.0 bits (715), Expect = 2.4e-72
Identity = 129/192 (67.19%), Postives = 161/192 (83.85%), Query Frame = 1


Query: 12  LSIGFNGGIPIDEEFYIKNIAGKNNDGIIRTLFPNDVEQGAEYMNEKEAMFRRLVVEQLK 71
           L IGFNGG+PI EEF+I  +AGK+ND I   LFP D+E+G +++++KEAMFRRL  EQLK
Sbjct: 3   LEIGFNGGVPISEEFFIDTVAGKHNDDIALVLFPGDLERGLKFVDDKEAMFRRLAAEQLK 62

Query: 72  PVSGLYKVKKWVEDHRLKCAAVTNSSRINAELMISMLGLTDFFETIIIGDECEHPKPHPE 131
           P++GL KV+KW+E+H LK AAVTN+ R NAELMIS+LGL+DFF+ +IIG ECEH KPHP+
Sbjct: 63  PLNGLDKVRKWIENHGLKRAAVTNAPRANAELMISILGLSDFFDAVIIGGECEHAKPHPD 122

Query: 132 PYLKALEALQVSKDHTFIFEDSLTGIKAGVEADMPVVGISMRNSEQLLMQAKPALLVKDY 191
           PYLK LEAL+ SKDHTF+FEDS++GIKAGV A MPV+G++ RN E LLM+AKPA L+KDY
Sbjct: 123 PYLKGLEALKASKDHTFVFEDSVSGIKAGVAAGMPVIGLATRNPENLLMEAKPAFLIKDY 182

Query: 192 NDLKLWLALDEL 204
            D KLW AL+EL
Sbjct: 183 EDPKLWAALEEL 194

[Close]

BLAST of CSPI03G02020 vs. TAIR10
Match: AT2G38740.1 (AT2G38740.1 Haloacid dehalogenase-like hydrolase (HAD) superfamily protein)

HSP 1 Score: 253.4 bits (646), Expect = 1.2e-67
Identity = 116/190 (61.05%), Postives = 152/190 (80.00%), Query Frame = 1


Query: 14  IGFNGGIPIDEEFYIKNIAGKNNDGIIRTLFPNDVEQGAEYMNEKEAMFRRLVVEQLKPV 73
           IGFN G+PIDE+F+++NIAGK+N  I   LFP+DV +G ++ +EKEA++R++V E++KP+
Sbjct: 51  IGFNNGVPIDEKFFVENIAGKHNSEIALLLFPDDVSRGLKFCDEKEALYRKIVAEKIKPL 110

Query: 74  SGLYKVKKWVEDHRLKCAAVTNSSRINAELMISMLGLTDFFETIIIGDECEHPKPHPEPY 133
            GL K+ KW+ED  LK AAVTN+ + NAELMIS LGLTDFF+ +I+G ECE PKPHP PY
Sbjct: 111 DGLIKLTKWIEDRGLKRAAVTNAPKENAELMISKLGLTDFFQAVILGSECEFPKPHPGPY 170

Query: 134 LKALEALQVSKDHTFIFEDSLTGIKAGVEADMPVVGISMRNSEQLLMQAKPALLVKDYND 193
           LKALE L VSK+HT +FEDS++GIKAGV A MPV+G++  N   LLMQAKPA L+++Y D
Sbjct: 171 LKALEVLNVSKEHTLVFEDSISGIKAGVAAGMPVIGLTTGNPASLLMQAKPAFLIENYAD 230

Query: 194 LKLWLALDEL 204
            KLW  L+EL
Sbjct: 231 PKLWAVLEEL 240

[Close]

BLAST of CSPI03G02020 vs. NCBI nr
Match: gi|449464920|ref|XP_004150177.1| (PREDICTED: haloacid dehalogenase-like hydrolase domain-containing protein Sgpp [Cucumis sativus])

HSP 1 Score: 404.4 bits (1038), Expect = 1.2e-109
Identity = 200/200 (100.00%), Postives = 200/200 (100.00%), Query Frame = 1


Query: 14  IGFNGGIPIDEEFYIKNIAGKNNDGIIRTLFPNDVEQGAEYMNEKEAMFRRLVVEQLKPV 73
           IGFNGGIPIDEEFYIKNIAGKNNDGIIRTLFPNDVEQGAEYMNEKEAMFRRLVVEQLKPV
Sbjct: 51  IGFNGGIPIDEEFYIKNIAGKNNDGIIRTLFPNDVEQGAEYMNEKEAMFRRLVVEQLKPV 110

Query: 74  SGLYKVKKWVEDHRLKCAAVTNSSRINAELMISMLGLTDFFETIIIGDECEHPKPHPEPY 133
           SGLYKVKKWVEDHRLKCAAVTNSSRINAELMISMLGLTDFFETIIIGDECEHPKPHPEPY
Sbjct: 111 SGLYKVKKWVEDHRLKCAAVTNSSRINAELMISMLGLTDFFETIIIGDECEHPKPHPEPY 170

Query: 134 LKALEALQVSKDHTFIFEDSLTGIKAGVEADMPVVGISMRNSEQLLMQAKPALLVKDYND 193
           LKALEALQVSKDHTFIFEDSLTGIKAGVEADMPVVGISMRNSEQLLMQAKPALLVKDYND
Sbjct: 171 LKALEALQVSKDHTFIFEDSLTGIKAGVEADMPVVGISMRNSEQLLMQAKPALLVKDYND 230

Query: 194 LKLWLALDELVVRGDTIANA 214
           LKLWLALDELVVRGDTIANA
Sbjct: 231 LKLWLALDELVVRGDTIANA 250

[Close]

BLAST of CSPI03G02020 vs. NCBI nr
Match: gi|449464904|ref|XP_004150169.1| (PREDICTED: haloacid dehalogenase-like hydrolase domain-containing protein Sgpp [Cucumis sativus])

HSP 1 Score: 313.9 bits (803), Expect = 2.1e-82
Identity = 152/200 (76.00%), Postives = 172/200 (86.00%), Query Frame = 1


Query: 14  IGFNGGIPIDEEFYIKNIAGKNNDGIIRTLFPNDVEQGAEYMNEKEAMFRRLVVEQLKPV 73
           IGFNGG+PIDEEF+IKN+AGK+ND I R LFP+D E+G ++ +EKEAMFRRLV EQLKPV
Sbjct: 51  IGFNGGVPIDEEFFIKNVAGKHNDDIARALFPDDFERGLKFCDEKEAMFRRLVTEQLKPV 110

Query: 74  SGLYKVKKWVEDHRLKCAAVTNSSRINAELMISMLGLTDFFETIIIGDECEHPKPHPEPY 133
            GLYKVKKW+ED  LK AAVTN+ R NAELMISMLGL DFFE IIIG EC+H KPHPEPY
Sbjct: 111 DGLYKVKKWIEDRGLKRAAVTNAPRPNAELMISMLGLKDFFEAIIIGGECKHAKPHPEPY 170

Query: 134 LKALEALQVSKDHTFIFEDSLTGIKAGVEADMPVVGISMRNSEQLLMQAKPALLVKDYND 193
           LKALE L+VSK+HTFIFEDS +GIKAGV A+MPVVGI+ RN EQLLMQAKP L+VKDY+D
Sbjct: 171 LKALEVLKVSKEHTFIFEDSASGIKAGVAAEMPVVGIATRNPEQLLMQAKPTLVVKDYDD 230

Query: 194 LKLWLALDELVVRGDTIANA 214
           LKLW ALDEL  RG T+ NA
Sbjct: 231 LKLWAALDELDKRGGTVKNA 250

[Close]

BLAST of CSPI03G02020 vs. NCBI nr
Match: gi|659095747|ref|XP_008448747.1| (PREDICTED: haloacid dehalogenase-like hydrolase domain-containing protein Sgpp [Cucumis melo])

HSP 1 Score: 307.4 bits (786), Expect = 2.0e-80
Identity = 148/200 (74.00%), Postives = 172/200 (86.00%), Query Frame = 1


Query: 14  IGFNGGIPIDEEFYIKNIAGKNNDGIIRTLFPNDVEQGAEYMNEKEAMFRRLVVEQLKPV 73
           IG+NGG+PIDEEF+IKN+AGK+ND I R LFP+D E+G ++ +EKEAMFRRLV EQLKPV
Sbjct: 51  IGYNGGVPIDEEFFIKNVAGKHNDDIARALFPDDFERGLKFCDEKEAMFRRLVTEQLKPV 110

Query: 74  SGLYKVKKWVEDHRLKCAAVTNSSRINAELMISMLGLTDFFETIIIGDECEHPKPHPEPY 133
           +GLYKV KW+ED  LK AAVTN+ R NAELMIS+LGL DFFE IIIG EC+H KPHPEPY
Sbjct: 111 NGLYKVTKWIEDRGLKRAAVTNAPRPNAELMISILGLKDFFEAIIIGGECKHAKPHPEPY 170

Query: 134 LKALEALQVSKDHTFIFEDSLTGIKAGVEADMPVVGISMRNSEQLLMQAKPALLVKDYND 193
           LKALE L+VSK+HTFIFEDS++GIKAGV A+MPVVGI+ RN EQLLMQAKP L+VKDY+D
Sbjct: 171 LKALEVLKVSKEHTFIFEDSVSGIKAGVAAEMPVVGIATRNPEQLLMQAKPTLVVKDYDD 230

Query: 194 LKLWLALDELVVRGDTIANA 214
            KLW ALDEL  RG T+ NA
Sbjct: 231 PKLWAALDELDKRGGTVKNA 250

[Close]

BLAST of CSPI03G02020 vs. NCBI nr
Match: gi|1012355836|gb|KYP67023.1| (Phosphorylated carbohydrates phosphatase TM-1254, partial [Cajanus cajan])

HSP 1 Score: 286.6 bits (732), Expect = 3.6e-74
Identity = 133/192 (69.27%), Postives = 164/192 (85.42%), Query Frame = 1


Query: 12  LSIGFNGGIPIDEEFYIKNIAGKNNDGIIRTLFPNDVEQGAEYMNEKEAMFRRLVVEQLK 71
           L IGFNGG+PIDEEF+I+ +AGK+ND I   LFP D+E+G +++++KEAMFRRL  EQLK
Sbjct: 37  LEIGFNGGVPIDEEFFIETVAGKHNDDIALALFPGDLERGLKFVDDKEAMFRRLAAEQLK 96

Query: 72  PVSGLYKVKKWVEDHRLKCAAVTNSSRINAELMISMLGLTDFFETIIIGDECEHPKPHPE 131
           P++GL KVKKW+E+H LK AAVTN+ R NAELMIS+LGL+DFFE IIIG ECEH KPHP+
Sbjct: 97  PLNGLDKVKKWIENHGLKRAAVTNAPRANAELMISLLGLSDFFEAIIIGGECEHAKPHPD 156

Query: 132 PYLKALEALQVSKDHTFIFEDSLTGIKAGVEADMPVVGISMRNSEQLLMQAKPALLVKDY 191
           PYLK LEAL+ SKDHTF+FEDS++GIKAGV A MPV+G++ RN E LLM+AKPA L+KDY
Sbjct: 157 PYLKGLEALKASKDHTFVFEDSVSGIKAGVAAGMPVIGLATRNPESLLMEAKPAFLIKDY 216

Query: 192 NDLKLWLALDEL 204
           +D KLW AL+EL
Sbjct: 217 DDPKLWAALEEL 228

[Close]

BLAST of CSPI03G02020 vs. NCBI nr
Match: gi|645246807|ref|XP_008229524.1| (PREDICTED: haloacid dehalogenase-like hydrolase domain-containing protein Sgpp [Prunus mume])

HSP 1 Score: 283.9 bits (725), Expect = 2.4e-73
Identity = 131/190 (68.95%), Postives = 160/190 (84.21%), Query Frame = 1


Query: 14  IGFNGGIPIDEEFYIKNIAGKNNDGIIRTLFPNDVEQGAEYMNEKEAMFRRLVVEQLKPV 73
           IGFNGG+PI EE+YI+N AGK+ND + R LFP+D E+G ++  +KEAMFRRL  EQLKPV
Sbjct: 51  IGFNGGVPITEEYYIENFAGKHNDDVARILFPDDFERGLKFTEDKEAMFRRLATEQLKPV 110

Query: 74  SGLYKVKKWVEDHRLKCAAVTNSSRINAELMISMLGLTDFFETIIIGDECEHPKPHPEPY 133
           +G+YKVKKWVED  LK AAVTN+ R NAELM+S+LGLTDFF+ +IIG+ECEH KPHPEPY
Sbjct: 111 NGIYKVKKWVEDRGLKRAAVTNAPRPNAELMLSILGLTDFFQALIIGNECEHAKPHPEPY 170

Query: 134 LKALEALQVSKDHTFIFEDSLTGIKAGVEADMPVVGISMRNSEQLLMQAKPALLVKDYND 193
           LKALE L VSKDHTF+FEDS++GIKAGV A MPV+G++  N E +L++AKP  L+KDYND
Sbjct: 171 LKALEILNVSKDHTFVFEDSVSGIKAGVAAGMPVIGLTTGNPEHILIEAKPTFLIKDYND 230

Query: 194 LKLWLALDEL 204
            KLW AL+EL
Sbjct: 231 SKLWAALEEL 240

The following BLAST results are available for this feature:

BLAST of CSPI03G02020 vs. Swiss-Prot


Match Name	E-value	Identity	Description
SGGP_ARATH	2.1e-66	61.05	Haloacid dehalogenase-like hydrolase domain-containing protein Sgpp OS=Arabidops...	[more]
PPAX_BACHD	2.0e-11	31.85	Pyrophosphatase PpaX OS=Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / F...	[more]
GPH_NITOC	1.1e-09	24.53	Phosphoglycolate phosphatase OS=Nitrosococcus oceani (strain ATCC 19707 / NCIMB ...	[more]
PPAX_BACWK	1.2e-08	28.13	Pyrophosphatase PpaX OS=Bacillus weihenstephanensis (strain KBAB4) GN=ppaX PE=3 ...	[more]
PPAX_BACC2	3.5e-08	26.56	Pyrophosphatase PpaX OS=Bacillus cereus (strain G9842) GN=ppaX PE=3 SV=1	[more]

BLAST of CSPI03G02020 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L202_CUCSA	8.3e-110	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G016920 PE=4 SV=1	[more]
A0A0A0L429_CUCSA	1.5e-82	76.00	2-deoxyglucose-6-phosphate phosphatase OS=Cucumis sativus GN=Csa_3G016930 PE=4 S...	[more]
A0A151TJ16_CAJCA	2.5e-74	69.27	Phosphorylated carbohydrates phosphatase TM-1254 (Fragment) OS=Cajanus cajan GN=...	[more]
W9S5E8_9ROSA	1.8e-72	70.00	Phosphorylated carbohydrates phosphatase OS=Morus notabilis GN=L484_023341 PE=4 ...	[more]
I1J9M8_SOYBN	2.4e-72	67.19	Uncharacterized protein OS=Glycine max GN=GLYMA_01G202000 PE=4 SV=1	[more]

BLAST of CSPI03G02020 vs. TAIR10


Match Name	E-value	Identity	Description
AT2G38740.1	1.2e-67	61.05	Haloacid dehalogenase-like hydrolase (HAD) superfamily protein	[more]

BLAST of CSPI03G02020 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449464920\|ref\|XP_004150177.1\|	1.2e-109	100.00	PREDICTED: haloacid dehalogenase-like hydrolase domain-containing protein Sgpp [...	[more]
gi\|449464904\|ref\|XP_004150169.1\|	2.1e-82	76.00	PREDICTED: haloacid dehalogenase-like hydrolase domain-containing protein Sgpp [...	[more]
gi\|659095747\|ref\|XP_008448747.1\|	2.0e-80	74.00	PREDICTED: haloacid dehalogenase-like hydrolase domain-containing protein Sgpp [...	[more]
gi\|1012355836\|gb\|KYP67023.1\|	3.6e-74	69.27	Phosphorylated carbohydrates phosphatase TM-1254, partial [Cajanus cajan]	[more]
gi\|645246807\|ref\|XP_008229524.1\|	2.4e-73	68.95	PREDICTED: haloacid dehalogenase-like hydrolase domain-containing protein Sgpp [...	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR006439	HAD-SF_hydro_IA
IPR023214	HAD_sf

Vocabulary: **Biological Process**
Term	Definition
GO:0008152	metabolic process

Vocabulary: **Molecular Function**
Term	Definition
GO:0016787	hydrolase activity

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016310	phosphorylation
biological_process	GO:0009231	riboflavin biosynthetic process
biological_process	GO:0008152	metabolic process
biological_process	GO:0046487	glyoxylate metabolic process
biological_process	GO:0006470	protein dephosphorylation
cellular_component	GO:0005575	cellular_component
cellular_component	GO:0005829	cytosol
cellular_component	GO:0005886	plasma membrane
molecular_function	GO:0016787	hydrolase activity
molecular_function	GO:0008531	riboflavin kinase activity
molecular_function	GO:0008967	phosphoglycolate phosphatase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G02020.1	CSPI03G02020.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006439	HAD hydrolase, subfamily IA	TIGRFAMs	TIGR01509	TIGR01509	coord: 90..170 score: 2.1
IPR023214	HAD-like domain	GENE3D	G3DSA:3.40.50.1000		coord: 52..198 score: 1.8
IPR023214	HAD-like domain	PFAM	PF13419	HAD_2	coord: 27..169 score: 8.3
IPR023214	HAD-like domain	unknown	SSF56784	HAD-like	coord: 19..195 score: 1.47
None	No IPR available	PANTHER	PTHR18901	2-DEOXYGLUCOSE-6-PHOSPHATE PHOSPHATASE 2	coord: 19..210 score: 3.2E
None	No IPR available	PANTHER	PTHR18901:SF42	HALOACID DEHALOGENASE-LIKE HYDROLASE DOMAIN-CONTAINING PROTEIN SGPP	coord: 19..210 score: 3.2E

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CSPI03G02020	Cla008775	Watermelon (97103) v1	cpiwmB200
CSPI03G02020	Csa3G016920	Cucumber (Chinese Long) v2	cpicuB120
CSPI03G02020	MELO3C013878	Melon (DHL92) v3.5.1	cpimeB240
CSPI03G02020	ClCG10G001930	Watermelon (Charleston Gray)	cpiwcgB190
CSPI03G02020	Lsi07G012320	Bottle gourd (USVL1VR-Ls)	cpilsiB227
CSPI03G02020	MELO3C013877.2	Melon (DHL92) v3.6.1	cpimedB225
CSPI03G02020	CsaV3_3G002060	Cucumber (Chinese Long) v3	cpicucB144
CSPI03G02020	Cla97C10G186560	Watermelon (97103) v2	cpiwmbB184
CSPI03G02020	Bhi11G000100	Wax gourd	cpiwgoB239
CSPI03G02020	Cucsa.321540	Cucumber (Gy14) v1	cgycpiB484
CSPI03G02020	CmaCh06G008380	Cucurbita maxima (Rimu)	cmacpiB838
CSPI03G02020	CmoCh16G011360	Cucurbita moschata (Rifu)	cmocpiB316
CSPI03G02020	CmoCh06G008600	Cucurbita moschata (Rifu)	cmocpiB823
CSPI03G02020	Cp4.1LG14g08950	Cucurbita pepo (Zucchini)	cpecpiB207
CSPI03G02020	Cp4.1LG00g03740	Cucurbita pepo (Zucchini)	cpecpiB010
CSPI03G02020	CsGy3G001900	Cucumber (Gy14) v2	cgybcpiB110
CSPI03G02020	Carg23232	Silver-seed gourd	carcpiB0742
CSPI03G02020	Carg12071	Silver-seed gourd	carcpiB0597

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CSPI03G02020	Wild cucumber (PI 183967)	cpicpiB100
CSPI03G02020	Wild cucumber (PI 183967)	cpicpiB103
CSPI03G02020	Cucumber (Gy14) v1	cgycpiB474
CSPI03G02020	Cucurbita maxima (Rimu)	cmacpiB262
CSPI03G02020	Cucurbita maxima (Rimu)	cmacpiB325
CSPI03G02020	Cucurbita maxima (Rimu)	cmacpiB608
CSPI03G02020	Cucurbita moschata (Rifu)	cmocpiB597
CSPI03G02020	Cucumber (Chinese Long) v2	cpicuB122
CSPI03G02020	Cucumber (Chinese Long) v2	cpicuB125
CSPI03G02020	Melon (DHL92) v3.5.1	cpimeB218
CSPI03G02020	Melon (DHL92) v3.5.1	cpimeB238
CSPI03G02020	Watermelon (Charleston Gray)	cpiwcgB180
CSPI03G02020	Watermelon (Charleston Gray)	cpiwcgB235
CSPI03G02020	Watermelon (97103) v1	cpiwmB184
CSPI03G02020	Watermelon (97103) v1	cpiwmB240
CSPI03G02020	Cucurbita pepo (Zucchini)	cpecpiB720
CSPI03G02020	Bottle gourd (USVL1VR-Ls)	cpilsiB218
CSPI03G02020	Melon (DHL92) v3.6.1	cpimedB213
CSPI03G02020	Cucumber (Gy14) v2	cgybcpiB113
CSPI03G02020	Silver-seed gourd	carcpiB0818
CSPI03G02020	Cucumber (Chinese Long) v3	cpicucB146
CSPI03G02020	Watermelon (97103) v2	cpiwmbB261