CSPI03G01900 (gene) Wild cucumber (PI 183967) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CSPI03G01900
Type	gene
Organism	Cucumis sativus (Wild cucumber (PI 183967))
Description	Cytochrome P450 family ent-kaurenoic acid oxidase
Location	Chr3 : 1338342 .. 1341455 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTACAAGACTCACATATTTGGAAAACCAACTATAATCGTCTGCAAACCTGAAATATGTCGTCAAATATTAACTGATGAAACCAAGTTCATTCCTAGTTATCCTACATCCATCACAGCCGTATTTGGTAAAAAATCACTGCTTCAAGTACCGAAAGAGGAGCATCGGAAGCTCCGACGGCTGACAATGGCACCGATCAGTGGCCATGCAGCGCTCGAGATGTACATTGATCATATTGAACACACTGTGATTAGTGGGTTGGAGGAGTGGTCAAGCATGAAGAAGCCATTGGAATTATTAACAACGATAAAGCAACTTACCTTCAAAGTTATTTGGAACATTTTTATGGGTTCAACTCCAATAAAAAGCACTTCTATAGGAGAAATGGAGACTTTGAATGAGAATATTGCACTTGCGTTCCTCACTCTGCCACTCAACTTTCCTGGCTTACCCTTCCATAAAGCACTCATGGTAAGAACAAAAATAATTTCTAATACAAATGTGACGAAGTGTCGATAACTTTTATCATATATGTAAAATATATTTTCAATTAATCTACTCTACAATAATCACTATATCATATGATACAACGAAAAGAAATGTATAAATTATATGTAGGCTGGAAAAAGATTGAATGAAATACTTCAATCATATTTGGATGAGAAGAGAATGGTGAAGAAAAGCAAAGGGGAAAATTGGGAAGCAAAAGATATGATGGATTTGTTGATGGAAGTGAGAGATGAAGATGGTGAAGGATTTGATGATGAGACCATTACAGAGATAATTTTTGGTATGCTATTTGGAGGCCAAGAGACTACAGCCTTCACTACAATGTGGGCAGTTTTGTTTCTTACGGATAATCCACATATATTCCAGAAGGCCAAGGTTAGACTCTTCTTTAATTTCCCAACTCGTGTTTTAATTGCATAATAATGTATATGAGATAAATGAAACTGAATTAAGATATATATGATCAACGATTATACTACAAATTCACATAGATATTTTAGGGTATGCTATTTTGATTTCGAGATTCATATTAAAGAGACGTTATATGAATGTACCAATAATATAGTTGAGATATCCTAGTGTGTATCTATTGATTCTCGTTCTTGGTGTTTTTTTTTTCTGAAAAAAAAAAGCATATTTAATTTGTATTGGTTGTTTGAAAAAATTATCAATATCGACAAAAAGATCGAAACGTTACTTTTGTAAAAATATAAATGAAAAGTTTGGTTAAAATATTTGTGTCGATGGTATGAGATATAAATTGCATAATAATGTGTATGAGATATAAATGAAACTTAATTAAGATATATATGATCAAGATTTAGACTACATATCATATCGAAATTTTAAAGTATGCTATTTTGATTTTGAGATACATGATAAAGAGACGCTATATATATGATGTTTTTCTCGGAAAAAAAAAAGCATATTTGTGTTGGTTGTTTGAAAAATATATCAATACTGACAAAACTATCAAAACACTACTTTTGTAAAAATATGGATGAAAATGTATCGTTGTTAAAATGTTGGTGTCAAGGGACATTACTTAGAAAATTAGAAAAGTAAAAAAATAAAAAACAAATTTAAATTAGTAAATAAACCTATTATTATGATATTTAAACCAGATTAGCATGTTTATTATTATTTATATTACACAATTTATTATATATATATATTGATTCATTTCAAGTCGATGTCAAACTGTTTGAAACGTAAAAAATATTGACATATCAACAGAACATCTGAAAATTTAATACAACAGACATATGATCAAGATATGGATAACATAGTAATCTAGTATTTTTTTTATTAATTTTATTATTATGAAATGAATTATTATTTTGATGGTTAAGTGTAAGATTGGTTTTAGGAAGAACAAGAAGATATCATTAGAAGAAGAGCCTCAACACAAAAGGGTGTAAGTCTGTCAGAGATTAAACAAATGAAATTTCTTTCTCAGGTATTTTAATAATTTTAATTAATTTCCTTTGATTTTTATTTTAATTTTTAGAACATTAATTTGGGTTGGTAATCAATTTTGATTTCAGAATGTTTTTAAATTATTAACATAAAAGAATTAGTATTAAAATTCTAAATGATTAATTGTTGAGTTTTCTTTTATGGTTGTTGATCCGTTCATGATTAAAGACTTGTCCTCTTCCTTAAAATATTCTCTAATTAGTTTGTTAGCACTATTGTTTTAAATATCAATGCTTTTGGTTACCTCTGTTTTCTTTTTTGGGCATTGGACTTATCTCTAGTCCTTTTTGTTCTCTCTTGCTTCTTGTACTCTCCCTTCTTATATTAATGAAGTGGAGATGATGAGGGTGCTAAGGGAGTGTCTACCTAGTGGAGATGTTCGGGTGCACCTATTAACCCGTATCTGTATCTTTTTTAAAATAAAAGACTTGTCCTCTTAACAAATTTATGAAATATAACTTTTTGATTGAGCAATAGAACATTTCCATATTTTTTAAAGATAATCATATGTATCGTAGTCCTAATTAGATCGTGATGCCTATCTATTGCAATTATAGGCTATAAAGATTATGATTTCCCCACTCTATATTCACGTAACCTTACCCCTAATGCATAATGATTGAGATTTTCATGAATGTCCCCTGTATTTGTGGAATATTTTAACAAACTATGATAAAAAAAAAAAAAAATGTCATAGAAATGGTCATCCCTAAGACTAAGTAAAAAAGTTAGACTACATGATTAAAAGTTTGTGAGAGGACGGATCATTGATTTGTATTTATGTTACCAAGAAGTATCTAAATATAGTAACTAGAATTGCTATAGTATATGGCTCAACTAGAAAGGGGCTTTGTAATCAGAGTTTTGAGTTTCTCTCTAGTTTGTTTTGTACATTTCCATTAATCAATGATTTTACTTTTGGTCTTGGGACATGATGAGGATGTTATAAAGAGATATCAACTTAGTTGAGATATCTTGATGTATCTCCTAATCCACTCGTTTTGTATATATTTTCAAAATCTAGTAATATTGAATTATAACTATTAATTCTATATACTTTATGTATAATATTCAGGTTATAGACTAGACGCTTCGCTTAAGTAGTGTGGTATTTGCTGTTTTTCG

mRNA sequence

ATGTACAAGACTCACATATTTGGAAAACCAACTATAATCGTCTGCAAACCTGAAATATGTCGTCAAATATTAACTGATGAAACCAAGTTCATTCCTAGTTATCCTACATCCATCACAGCCGTATTTGGTAAAAAATCACTGCTTCAAGTACCGAAAGAGGAGCATCGGAAGCTCCGACGGCTGACAATGGCACCGATCAGTGGCCATGCAGCGCTCGAGATGTACATTGATCATATTGAACACACTGTGATTAGTGGGTTGGAGGAGTGGTCAAGCATGAAGAAGCCATTGGAATTATTAACAACGATAAAGCAACTTACCTTCAAAGTTATTTGGAACATTTTTATGGGTTCAACTCCAATAAAAAGCACTTCTATAGGAGAAATGGAGACTTTGAATGAGAATATTGCACTTGCGTTCCTCACTCTGCCACTCAACTTTCCTGGCTTACCCTTCCATAAAGCACTCATGGCTGGAAAAAGATTGAATGAAATACTTCAATCATATTTGGATGAGAAGAGAATGGTGAAGAAAAGCAAAGGGGAAAATTGGGAAGCAAAAGATATGATGGATTTGTTGATGGAAGTGAGAGATGAAGATGGTGAAGGATTTGATGATGAGACCATTACAGAGATAATTTTTGGTATGCTATTTGGAGGCCAAGAGACTACAGCCTTCACTACAATGTGGGCAGTTTTGTTTCTTACGGATAATCCACATATATTCCAGAAGGCCAAGGAAGAACAAGAAGATATCATTAGAAGAAGAGCCTCAACACAAAAGGGTGTAAGTCTGTCAGAGATTAAACAAATGAAATTTCTTTCTCAGGTTATAGACTAG

Coding sequence (CDS)

ATGTACAAGACTCACATATTTGGAAAACCAACTATAATCGTCTGCAAACCTGAAATATGTCGTCAAATATTAACTGATGAAACCAAGTTCATTCCTAGTTATCCTACATCCATCACAGCCGTATTTGGTAAAAAATCACTGCTTCAAGTACCGAAAGAGGAGCATCGGAAGCTCCGACGGCTGACAATGGCACCGATCAGTGGCCATGCAGCGCTCGAGATGTACATTGATCATATTGAACACACTGTGATTAGTGGGTTGGAGGAGTGGTCAAGCATGAAGAAGCCATTGGAATTATTAACAACGATAAAGCAACTTACCTTCAAAGTTATTTGGAACATTTTTATGGGTTCAACTCCAATAAAAAGCACTTCTATAGGAGAAATGGAGACTTTGAATGAGAATATTGCACTTGCGTTCCTCACTCTGCCACTCAACTTTCCTGGCTTACCCTTCCATAAAGCACTCATGGCTGGAAAAAGATTGAATGAAATACTTCAATCATATTTGGATGAGAAGAGAATGGTGAAGAAAAGCAAAGGGGAAAATTGGGAAGCAAAAGATATGATGGATTTGTTGATGGAAGTGAGAGATGAAGATGGTGAAGGATTTGATGATGAGACCATTACAGAGATAATTTTTGGTATGCTATTTGGAGGCCAAGAGACTACAGCCTTCACTACAATGTGGGCAGTTTTGTTTCTTACGGATAATCCACATATATTCCAGAAGGCCAAGGAAGAACAAGAAGATATCATTAGAAGAAGAGCCTCAACACAAAAGGGTGTAAGTCTGTCAGAGATTAAACAAATGAAATTTCTTTCTCAGGTTATAGACTAG

Homology

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BLAST of CSPI03G01900 vs. Swiss-Prot
Match: BAMO_GLYUR (Beta-amyrin 11-oxidase OS=Glycyrrhiza uralensis GN=CYP88D6 PE=1 SV=1)

HSP 1 Score: 261.9 bits (668), Expect = 7.9e-69
Identity = 131/280 (46.79%), Postives = 196/280 (70.00%), Query Frame = 1


Query: 1   MYKTHIFGKPTIIVCKPEICRQILTDETKFIPSYPTSITAVFGKKSLLQVPKEEHRKLRR 60
           +YKTH+FG P+IIVC+P++CR++LTD+  F   YP SI  +   + ++ V   EHR  RR
Sbjct: 84  IYKTHLFGNPSIIVCEPQMCRRVLTDDVNFKLGYPKSIKELARCRPMIDVSNAEHRLFRR 143

Query: 61  LTMAPISGHAALEMYIDHIEHTVISGLEEWSSMKKPLELLTTIKQLTFKVIWNIFMGSTP 120
           L  +PI GH AL MY++ +E  VI+ LEE SSMK P+ELL  +K+++FK I ++FMGS+ 
Sbjct: 144 LITSPIVGHKALAMYLERLEEIVINSLEELSSMKHPVELLKEMKKVSFKAIVHVFMGSS- 203

Query: 121 IKSTSIGEMETLNENIALAFLTLPLNFPGLPFHKALMAGKRLNEILQSYLDEKR-MVKKS 180
                I ++ +   ++     ++P+N PG  FHKAL A K+L +I+Q  +DE+R M++  
Sbjct: 204 -NQDIIKKIGSSFTDLYNGMFSIPINVPGFTFHKALEARKKLAKIVQPVVDERRLMIENG 263

Query: 181 KGENWEAKDMMDLLMEVRDEDGEGFDDETITEIIFGMLFGGQETTAFTTMWAVLFLTDNP 240
             E  + KD++D+L+EV+DE+G   +DE I++++ G+LF G E+TA + MW++ +LT +P
Sbjct: 264 PQEGSQRKDLIDILLEVKDENGRKLEDEDISDLLIGLLFAGHESTATSLMWSITYLTQHP 323

Query: 241 HIFQKAKEEQEDIIRRRASTQKGVSLSEIKQMKFLSQVID 280
           HI +KAKEEQE+I R R S+QK +SL EIKQM +LSQVID
Sbjct: 324 HILKKAKEEQEEITRTRFSSQKQLSLKEIKQMVYLSQVID 361

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BLAST of CSPI03G01900 vs. Swiss-Prot
Match: KAO1_ARATH (Ent-kaurenoic acid oxidase 1 OS=Arabidopsis thaliana GN=KAO1 PE=2 SV=1)

HSP 1 Score: 220.7 bits (561), Expect = 2.0e-56
Identity = 117/279 (41.94%), Postives = 175/279 (62.72%), Query Frame = 1


Query: 1   MYKTHIFGKPTIIVCKPEICRQILTDETKFIPSYPTSITAVFGKKSLLQVPKEEHRKLRR 60
           +YK H+FG P+IIV   + CR++LTD+  F P +PTS   + G+KS + +  EEH++LRR
Sbjct: 86  IYKAHMFGNPSIIVTTSDTCRRVLTDDDAFKPGWPTSTMELIGRKSFVGISFEEHKRLRR 145

Query: 61  LTMAPISGHAALEMYIDHIEHTVISGLEEWSSMKKPLELLTTIKQLTFKVIWNIFMGSTP 120
           LT AP++GH AL  YI +IE  VI+ L++W+ M +  E LT +++LTF++I  IF+ S  
Sbjct: 146 LTAAPVNGHEALSTYIPYIEENVITVLDKWTKMGE-FEFLTHLRKLTFRIIMYIFLSSES 205

Query: 121 IKSTSIGEMETLNENIALAFLTLPLNFPGLPFHKALMAGKRLNEILQSYLDEKRMVKKSK 180
                  E E    N  +    + +N PG  +H+AL A K L    QS + E+R  +K  
Sbjct: 206 ENVMDALEREYTALNYGVR--AMAVNIPGFAYHRALKARKTLVAAFQSIVTERRNQRKQN 265

Query: 181 GENWEAKDMMDLLMEVRDEDGEGFDDETITEIIFGMLFGGQETTAFTTMWAVLFLTDNPH 240
             +   KDM+D L+ V+DEDG+  DDE I +++   L  G E++  T MWA +FL ++P 
Sbjct: 266 ILS-NKKDMLDNLLNVKDEDGKTLDDEEIIDVLLMYLNAGHESSGHTIMWATVFLQEHPE 325

Query: 241 IFQKAKEEQEDIIRRRASTQKGVSLSEIKQMKFLSQVID 280
           + Q+AK EQE I++ R   QKG+SL E ++M+FLSQV+D
Sbjct: 326 VLQRAKAEQEMILKSRPEGQKGLSLKETRKMEFLSQVVD 360

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BLAST of CSPI03G01900 vs. Swiss-Prot
Match: KAO2_ARATH (Ent-kaurenoic acid oxidase 2 OS=Arabidopsis thaliana GN=KAO2 PE=2 SV=2)

HSP 1 Score: 209.5 bits (532), Expect = 4.6e-53
Identity = 116/279 (41.58%), Postives = 174/279 (62.37%), Query Frame = 1


Query: 1   MYKTHIFGKPTIIVCKPEICRQILTDETKFIPSYPTSITAVFGKKSLLQVPKEEHRKLRR 60
           +YK H+FG P ++V  PE CR++LTD+  F   +P S   + G+KS + +  EEH++LRR
Sbjct: 85  IYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRKSFVGISFEEHKRLRR 144

Query: 61  LTMAPISGHAALEMYIDHIEHTVISGLEEWSSMKKPLELLTTIKQLTFKVIWNIFMGSTP 120
           LT AP++G  AL +YI  IE TV + LE+WS M + +E L+ +++LTFKVI  IF+ S  
Sbjct: 145 LTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGE-IEFLSHLRKLTFKVIMYIFLSSES 204

Query: 121 IKSTSIGEMETLNENIALAFLTLPLNFPGLPFHKALMAGKRLNEILQSYLDEKRMVKKSK 180
                  E E  N N  +    + +N PG  +H+AL A K+L    QS +  +R  +K  
Sbjct: 205 EHVMDSLEREYTNLNYGVR--AMGINLPGFAYHRALKARKKLVAAFQSIVTNRRNQRKQN 264

Query: 181 GENWEAKDMMDLLMEVRDEDGEGFDDETITEIIFGMLFGGQETTAFTTMWAVLFLTDNPH 240
             +   KDM+D L++V+DE+G   DDE I +++   L  G E++   TMWA + + ++P 
Sbjct: 265 ISS-NRKDMLDNLIDVKDENGRVLDDEEIIDLLLMYLNAGHESSGHLTMWATILMQEHPM 324

Query: 241 IFQKAKEEQEDIIRRRASTQKGVSLSEIKQMKFLSQVID 280
           I QKAKEEQE I+++RA  QK ++L E ++M +LSQVID
Sbjct: 325 ILQKAKEEQERIVKKRAPGQK-LTLKETREMVYLSQVID 358

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BLAST of CSPI03G01900 vs. Swiss-Prot
Match: C88A1_MAIZE (Cytochrome P450 88A1 OS=Zea mays GN=CYP88A1 PE=2 SV=1)

HSP 1 Score: 205.3 bits (521), Expect = 8.7e-52
Identity = 106/279 (37.99%), Postives = 170/279 (60.93%), Query Frame = 1


Query: 1   MYKTHIFGKPTIIVCKPEICRQILTDETKFIPSYPTSITAVFGKKSLLQVPKEEHRKLRR 60
           +Y++ +F  PT++V   E C+Q+L D+  F+  +P +  A+ G +S + +P +EHR++R+
Sbjct: 113 VYRSFMFSSPTVLVTTAEGCKQVLMDDDAFVTGWPKATVALVGPRSFVAMPYDEHRRIRK 172

Query: 61  LTMAPISGHAALEMYIDHIEHTVISGLEEWSSMKKPLELLTTIKQLTFKVIWNIFMGSTP 120
           LT API+G  AL  Y+  I+ TV S L  W+     +E LT ++++TFK+I  IF+G   
Sbjct: 173 LTAAPINGFDALTGYLPFIDRTVTSSLRAWADHGGSVEFLTELRRMTFKIIVQIFLGGAD 232

Query: 121 IKSTSIGEMETLNENIALAFLTLPLNFPGLPFHKALMAGKRLNEILQSYLDEKRMVKKSK 180
             +T   E      N  +    + +N PG  +  AL A +RL  +LQ  LDE+R   ++K
Sbjct: 233 QATTRALERSYTELNYGMR--AMAINLPGFAYRGALRARRRLVAVLQGVLDERR-AARAK 292

Query: 181 GENWEAKDMMDLLMEVRDEDGEGFDDETITEIIFGMLFGGQETTAFTTMWAVLFLTDNPH 240
           G +    DMMD L+E +DE G   DD+ I +++   L  G E++   TMWA +FL +NP 
Sbjct: 293 GVSGGGVDMMDRLIEAQDERGRHLDDDEIIDVLVMYLNAGHESSGHITMWATVFLQENPD 352

Query: 241 IFQKAKEEQEDIIRRRASTQKGVSLSEIKQMKFLSQVID 280
           +F +AK EQE I+R   S+Q+G++L + ++M++LSQVID
Sbjct: 353 MFARAKAEQEAIMRSIPSSQRGLTLRDFRKMEYLSQVID 388

[Close]

BLAST of CSPI03G01900 vs. Swiss-Prot
Match: KAO1_HORVU (Ent-kaurenoic acid oxidase 1 OS=Hordeum vulgare GN=KAO1 PE=1 SV=1)

HSP 1 Score: 193.4 bits (490), Expect = 3.4e-48
Identity = 105/282 (37.23%), Postives = 161/282 (57.09%), Query Frame = 1


Query: 1   MYKTHIFGKPTIIVCKPEICRQILTDETKFIPSYPTSITAVFGKKSLLQVPKEEHRKLRR 60
           +Y+  +F  PTI+   PE C+Q+L D+  F+  +P +  A+ G KS + +  +EHR+LR+
Sbjct: 86  LYRAFMFSSPTIMATTPEACKQVLMDDDAFVTGWPKATVALIGPKSFVNMGYDEHRRLRK 145

Query: 61  LTMAPISGHAALEMYIDHIEHTVISGLEEWSSM--KKPLELLTTIKQLTFKVIWNIFMGS 120
           LT API+G  AL  Y+  I+ TV++ L  WS        E LT ++++TF++I  IFMG 
Sbjct: 146 LTAAPINGFDALTSYLGFIDDTVVTTLRGWSERGGDGHFEFLTELRRMTFRIIVQIFMGG 205

Query: 121 TPIKSTSIGEMETLNENIALAFLTLPLNFPGLPFHKALMAGKRLNEILQSYLDEKRMVKK 180
              ++ +  E+E     +      + ++ PG  +HKA+ A +RL   LQ  LDE+R    
Sbjct: 206 ADERTAA--ELERTYTELNYGMRAMAIDLPGFAYHKAIRARRRLVAALQRVLDERRARGG 265

Query: 181 SKGENWEAK-DMMDLLMEVRDEDGEGFDDETITEIIFGMLFGGQETTAFTTMWAVLFLTD 240
                  A  DMMD L+ V DE G    D+ I +++   L  G E++   TMWA +FL +
Sbjct: 266 KTAAGAAAPVDMMDRLIAVEDEGGRRLQDDEIIDVLVMYLNAGHESSGHITMWATVFLQE 325

Query: 241 NPHIFQKAKEEQEDIIRRRASTQKGVSLSEIKQMKFLSQVID 280
           NP I  KAK EQE I+R     QKG++L + ++M +LSQV+D
Sbjct: 326 NPEILAKAKAEQEAIMRSIPPGQKGLTLRDFRKMAYLSQVVD 365

[Close]

BLAST of CSPI03G01900 vs. TrEMBL
Match: A0A0A0L419_CUCSA (Cytochrome P450 88D6 OS=Cucumis sativus GN=Csa_3G015360 PE=4 SV=1)

HSP 1 Score: 481.5 bits (1238), Expect = 7.0e-133
Identity = 239/279 (85.66%), Postives = 255/279 (91.40%), Query Frame = 1


Query: 1   MYKTHIFGKPTIIVCKPEICRQILTDETKFIPSYPTSITAVFGKKSLLQVPKEEHRKLRR 60
           MYKTHIFGKPTIIVCKPE+CRQ+LTDE KFIP YPT++  +FGKKSL  V KEEHRKLRR
Sbjct: 1   MYKTHIFGKPTIIVCKPELCRQVLTDEAKFIPGYPTTMKTLFGKKSLHAVTKEEHRKLRR 60

Query: 61  LTMAPISGHAALEMYIDHIEHTVISGLEEWSSMKKPLELLTTIKQLTFKVIWNIFMGSTP 120
           L   PIS HAALEMYIDHIE TVISGLEEWSSM+KPLELLTTIKQLTFKVIWNIFMGSTP
Sbjct: 61  LITTPISSHAALEMYIDHIERTVISGLEEWSSMEKPLELLTTIKQLTFKVIWNIFMGSTP 120

Query: 121 IKSTSIGEMETLNENIALAFLTLPLNFPGLPFHKALMAGKRLNEILQSYLDEKRMVKKSK 180
           IKSTSI EMET N++I L F T+P+NFPG  FHKAL+A K L+EILQS LDEKRMVKKSK
Sbjct: 121 IKSTSIREMETFNDDIVLGFFTMPINFPGFSFHKALLARKGLHEILQSILDEKRMVKKSK 180

Query: 181 GENWEAKDMMDLLMEVRDEDGEGFDDETITEIIFGMLFGGQETTAFTTMWAVLFLTDNPH 240
           GENWEAKDMMDLLMEVRDEDGEGFDDETITE+IF MLFGGQET+AFT+MWAVLFLTDNPH
Sbjct: 181 GENWEAKDMMDLLMEVRDEDGEGFDDETITEMIFSMLFGGQETSAFTSMWAVLFLTDNPH 240

Query: 241 IFQKAKEEQEDIIRRRASTQKGVSLSEIKQMKFLSQVID 280
           IFQKAKEEQEDII+RRASTQ GVSLSEIKQMKFLSQVID
Sbjct: 241 IFQKAKEEQEDIIKRRASTQTGVSLSEIKQMKFLSQVID 279

[Close]

BLAST of CSPI03G01900 vs. TrEMBL
Match: A0A0A0L1B5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G011860 PE=3 SV=1)

HSP 1 Score: 387.1 bits (993), Expect = 1.8e-104
Identity = 192/279 (68.82%), Postives = 231/279 (82.80%), Query Frame = 1


Query: 1   MYKTHIFGKPTIIVCKPEICRQILTDETKFIPSYPTSITAVFGKKSLLQVPKEEHRKLRR 60
           MYKTH+ G+ +IIVC  E+CRQ+L DE KF PS P SIT + G+KSL+QV K EHR+LRR
Sbjct: 1   MYKTHLLGRASIIVCTSEVCRQVLCDEEKFKPSLPRSITLLSGRKSLMQVFKAEHRRLRR 60

Query: 61  LTMAPISGHAALEMYIDHIEHTVISGLEEWSSMKKPLELLTTIKQLTFKVIWNIFMGSTP 120
           LT APISGH+ALEMYI+HIE TVISGLEEW+SMK+P+ELLT IK+LTFK+IWNIFMGS+ 
Sbjct: 61  LTTAPISGHSALEMYINHIEQTVISGLEEWASMKRPIELLTEIKKLTFKIIWNIFMGSSS 120

Query: 121 IKSTSIGEMETLNENIALAFLTLPLNFPGLPFHKALMAGKRLNEILQSYLDEKRMVKKSK 180
           I S SIGE+E L   I L F++LP+N PG  F+K+L A K L  ILQS +++KRM KK +
Sbjct: 121 IDS-SIGEIEALFAKITLGFISLPINLPGFSFYKSLKARKELLTILQSIINQKRMAKKRE 180

Query: 181 GENWEAKDMMDLLMEVRDEDGEGFDDETITEIIFGMLFGGQETTAFTTMWAVLFLTDNPH 240
           GE WEAKDMMDLL+E++DEDGE  DDETI ++IFG LF G ETTA+T MWAVLFLT++PH
Sbjct: 181 GEGWEAKDMMDLLIELKDEDGEELDDETIRDLIFGKLFAGHETTAYTAMWAVLFLTNHPH 240

Query: 241 IFQKAKEEQEDIIRRRASTQKGVSLSEIKQMKFLSQVID 280
           I QKAKEEQEDII+RR +TQKG+ LSE+KQMKFL QVID
Sbjct: 241 ILQKAKEEQEDIIKRRPTTQKGLILSEVKQMKFLYQVID 278

[Close]

BLAST of CSPI03G01900 vs. TrEMBL
Match: A0A0A0L415_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G011850 PE=4 SV=1)

HSP 1 Score: 362.5 bits (929), Expect = 4.8e-97
Identity = 181/280 (64.64%), Postives = 224/280 (80.00%), Query Frame = 1


Query: 1   MYKTHIFGKPTIIVCKPEICRQILTDETKFIPSYPTSITAVFGKKSLLQVPKEEHRKLRR 60
           MYKTH+ G+P+IIV  PEICRQ+LT+E  F PS P +I  + G+KSL+QV K EHR+LRR
Sbjct: 84  MYKTHLLGRPSIIVYTPEICRQVLTNEEIFTPSLPKNIVILSGRKSLMQVTKAEHRRLRR 143

Query: 61  LTMAPISGHAALEMYIDHIEHTVISGLEEWSSMKKPLELLTTIKQLTFKVIWNIFMGSTP 120
           LT APIS H ALE+YI+ IE TV++GLEEW+SMK P+ELLT +K+LTFKVIWNIFMGST 
Sbjct: 144 LTTAPISSHTALEIYINDIEQTVMNGLEEWASMKSPIELLTEMKKLTFKVIWNIFMGST- 203

Query: 121 IKSTSIGEMET-LNENIALAFLTLPLNFPGLPFHKALMAGKRLNEILQSYLDEKRMVKKS 180
              +S+GE E+ L   +A  FL+LP+NFPG  F K+  A ++L E LQ  ++EKR +K+ 
Sbjct: 204 ---SSMGENESSLFYEVAAGFLSLPINFPGFGFRKSFKARQKLMERLQCIINEKRSMKER 263

Query: 181 KGENWEAKDMMDLLMEVRDEDGEGFDDETITEIIFGMLFGGQETTAFTTMWAVLFLTDNP 240
           KGENWEAKD MD L++V+DEDGE  DDETI ++IFG LF G ETTA+T MWAVLFLTD+P
Sbjct: 264 KGENWEAKDTMDFLIDVKDEDGEELDDETIRDLIFGKLFAGHETTAYTAMWAVLFLTDHP 323

Query: 241 HIFQKAKEEQEDIIRRRASTQKGVSLSEIKQMKFLSQVID 280
           H FQKAKEEQE++IRRR STQKG++ SE KQMK+LSQVID
Sbjct: 324 HTFQKAKEEQEELIRRRPSTQKGINFSEFKQMKYLSQVID 359

[Close]

BLAST of CSPI03G01900 vs. TrEMBL
Match: A0A0A0L1Y8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G014360 PE=4 SV=1)

HSP 1 Score: 316.2 bits (809), Expect = 3.9e-83
Identity = 154/162 (95.06%), Postives = 158/162 (97.53%), Query Frame = 1


Query: 1   MYKTHIFGKPTIIVCKPEICRQILTDETKFIPSYPTSITAVFGKKSLLQVPKEEHRKLRR 60
           MYKTHIFGKPT+IVCKPEICRQ+LTDETKFIPSYPTSITAVFGKKSLLQVPKEEHRKLRR
Sbjct: 84  MYKTHIFGKPTLIVCKPEICRQVLTDETKFIPSYPTSITAVFGKKSLLQVPKEEHRKLRR 143

Query: 61  LTMAPISGHAALEMYIDHIEHTVISGLEEWSSMKKPLELLTTIKQLTFKVIWNIFMGSTP 120
           LTMAPISGHAALEMYIDHIEHTVISGLEEWSSMKKPLELLTTIKQLTFKVIWNIFMGSTP
Sbjct: 144 LTMAPISGHAALEMYIDHIEHTVISGLEEWSSMKKPLELLTTIKQLTFKVIWNIFMGSTP 203

Query: 121 IKSTSIGEMETLNENIALAFLTLPLNFPGLPFHKALMAGKRL 163
           IKSTSI EMETLNENIALAFLTLPLNFPGLPFHKALM   ++
Sbjct: 204 IKSTSIREMETLNENIALAFLTLPLNFPGLPFHKALMVRTKI 245

[Close]

BLAST of CSPI03G01900 vs. TrEMBL
Match: A0A061G3A5_THECC (Cytochrome P450 88D6, putative OS=Theobroma cacao GN=TCM_015758 PE=3 SV=1)

HSP 1 Score: 264.6 bits (675), Expect = 1.4e-67
Identity = 136/279 (48.75%), Postives = 195/279 (69.89%), Query Frame = 1


Query: 1   MYKTHIFGKPTIIVCKPEICRQILTDETKFIPSYPTSITAVFGKKSLLQVPKEEHRKLRR 60
           +YKTH+FG P++IVC PE+CR++LTD+  F+  YP+S T + GKKSL  +   EHR+LRR
Sbjct: 193 IYKTHLFGSPSVIVCSPELCRKVLTDDEHFLFGYPSSATQLGGKKSLYGISNSEHRRLRR 252

Query: 61  LTMAPISGHAALEMYIDHIEHTVISGLEEWSSMKKPLELLTTIKQLTFKVIWNIFMGSTP 120
           LT  PI+GH AL +YI HIE  VI+ LE+   M +P++    +K++ FKVI  IF+GST 
Sbjct: 253 LTTDPINGHEALALYIGHIEDIVITSLEDLGGMNRPIKFFNEMKRIGFKVIAQIFLGST- 312

Query: 121 IKSTSIGEMETLNENIALAFLTLPLNFPGLPFHKALMAGKRLNEILQSYLDEKRMVKKSK 180
            + + +  M      +    L++P+N PG  FHKAL A K+L + +Q+ L  +R  +K+ 
Sbjct: 313 -QESVLSSMVKYYTELFPGVLSIPINLPGFAFHKALKARKKLVKTIQAELYVRR-AQKTM 372

Query: 181 GENWEAKDMMDLLMEVRDEDGEGFDDETITEIIFGMLFGGQETTAFTTMWAVLFLTDNPH 240
             N   K MMDLLMEV++E+GE  +DE I +++  +LF G ETTA T MWA++ L D+P 
Sbjct: 373 VPN-RKKGMMDLLMEVKNENGEKLEDEHIIDLLLLILFAGHETTAHTAMWAIIHLHDHPE 432

Query: 241 IFQKAKEEQEDIIRRRASTQKGVSLSEIKQMKFLSQVID 280
           + QKAKEEQE II+RR+S+QKG++L+EIKQM++L +VID
Sbjct: 433 MLQKAKEEQEAIIKRRSSSQKGLTLTEIKQMEYLPKVID 467

[Close]

BLAST of CSPI03G01900 vs. TAIR10
Match: AT1G05160.1 (AT1G05160.1 cytochrome P450, family 88, subfamily A, polypeptide 3)

HSP 1 Score: 220.7 bits (561), Expect = 1.1e-57
Identity = 117/279 (41.94%), Postives = 175/279 (62.72%), Query Frame = 1


Query: 1   MYKTHIFGKPTIIVCKPEICRQILTDETKFIPSYPTSITAVFGKKSLLQVPKEEHRKLRR 60
           +YK H+FG P+IIV   + CR++LTD+  F P +PTS   + G+KS + +  EEH++LRR
Sbjct: 86  IYKAHMFGNPSIIVTTSDTCRRVLTDDDAFKPGWPTSTMELIGRKSFVGISFEEHKRLRR 145

Query: 61  LTMAPISGHAALEMYIDHIEHTVISGLEEWSSMKKPLELLTTIKQLTFKVIWNIFMGSTP 120
           LT AP++GH AL  YI +IE  VI+ L++W+ M +  E LT +++LTF++I  IF+ S  
Sbjct: 146 LTAAPVNGHEALSTYIPYIEENVITVLDKWTKMGE-FEFLTHLRKLTFRIIMYIFLSSES 205

Query: 121 IKSTSIGEMETLNENIALAFLTLPLNFPGLPFHKALMAGKRLNEILQSYLDEKRMVKKSK 180
                  E E    N  +    + +N PG  +H+AL A K L    QS + E+R  +K  
Sbjct: 206 ENVMDALEREYTALNYGVR--AMAVNIPGFAYHRALKARKTLVAAFQSIVTERRNQRKQN 265

Query: 181 GENWEAKDMMDLLMEVRDEDGEGFDDETITEIIFGMLFGGQETTAFTTMWAVLFLTDNPH 240
             +   KDM+D L+ V+DEDG+  DDE I +++   L  G E++  T MWA +FL ++P 
Sbjct: 266 ILS-NKKDMLDNLLNVKDEDGKTLDDEEIIDVLLMYLNAGHESSGHTIMWATVFLQEHPE 325

Query: 241 IFQKAKEEQEDIIRRRASTQKGVSLSEIKQMKFLSQVID 280
           + Q+AK EQE I++ R   QKG+SL E ++M+FLSQV+D
Sbjct: 326 VLQRAKAEQEMILKSRPEGQKGLSLKETRKMEFLSQVVD 360

[Close]

BLAST of CSPI03G01900 vs. TAIR10
Match: AT2G32440.1 (AT2G32440.1 ent-kaurenoic acid hydroxylase 2)

HSP 1 Score: 209.5 bits (532), Expect = 2.6e-54
Identity = 116/279 (41.58%), Postives = 174/279 (62.37%), Query Frame = 1


Query: 1   MYKTHIFGKPTIIVCKPEICRQILTDETKFIPSYPTSITAVFGKKSLLQVPKEEHRKLRR 60
           +YK H+FG P ++V  PE CR++LTD+  F   +P S   + G+KS + +  EEH++LRR
Sbjct: 85  IYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRKSFVGISFEEHKRLRR 144

Query: 61  LTMAPISGHAALEMYIDHIEHTVISGLEEWSSMKKPLELLTTIKQLTFKVIWNIFMGSTP 120
           LT AP++G  AL +YI  IE TV + LE+WS M + +E L+ +++LTFKVI  IF+ S  
Sbjct: 145 LTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGE-IEFLSHLRKLTFKVIMYIFLSSES 204

Query: 121 IKSTSIGEMETLNENIALAFLTLPLNFPGLPFHKALMAGKRLNEILQSYLDEKRMVKKSK 180
                  E E  N N  +    + +N PG  +H+AL A K+L    QS +  +R  +K  
Sbjct: 205 EHVMDSLEREYTNLNYGVR--AMGINLPGFAYHRALKARKKLVAAFQSIVTNRRNQRKQN 264

Query: 181 GENWEAKDMMDLLMEVRDEDGEGFDDETITEIIFGMLFGGQETTAFTTMWAVLFLTDNPH 240
             +   KDM+D L++V+DE+G   DDE I +++   L  G E++   TMWA + + ++P 
Sbjct: 265 ISS-NRKDMLDNLIDVKDENGRVLDDEEIIDLLLMYLNAGHESSGHLTMWATILMQEHPM 324

Query: 241 IFQKAKEEQEDIIRRRASTQKGVSLSEIKQMKFLSQVID 280
           I QKAKEEQE I+++RA  QK ++L E ++M +LSQVID
Sbjct: 325 ILQKAKEEQERIVKKRAPGQK-LTLKETREMVYLSQVID 358

[Close]

BLAST of CSPI03G01900 vs. TAIR10
Match: AT2G29090.1 (AT2G29090.1 cytochrome P450, family 707, subfamily A, polypeptide 2)

HSP 1 Score: 119.4 bits (298), Expect = 3.6e-27
Identity = 75/279 (26.88%), Postives = 140/279 (50.18%), Query Frame = 1


Query: 1   MYKTHIFGKPTIIVCKPEICRQILTDETK-FIPSYPTSITAVFGKKSLLQVPKEEHRKLR 60
           ++KTHI G P +++  PE  R +L  +   F P+YP S   + G ++L       H  L+
Sbjct: 84  IFKTHILGCPCVMISSPEAARMVLVSKAHLFKPTYPPSKERMIGPEALFFHQGPYHSTLK 143

Query: 61  RLTMAPISGHAALEMYIDHIEHTVISGLEEWSSMKKPLELLTTIKQLTFKVIWNIFMGST 120
           RL  +     +AL   + HIE  V+  L  W+S K  +  L  +K+  F V      G  
Sbjct: 144 RLVQSSFMP-SALRPTVSHIELLVLQTLSSWTSQKS-INTLEYMKRYAFDVAIMSAFGDK 203

Query: 121 PIKSTSIGEMETLNENIALAFLTLPLNFPGLPFHKALMAGKRLNEILQSYLDEKRMVKKS 180
             + T+I  ++ L + +   + ++PL+ PG  FHK++ A   L+E L+  ++++R   + 
Sbjct: 204 E-EPTTIDVIKLLYQRLERGYNSMPLDLPGTLFHKSMKARIELSEELRKVIEKRRENGRE 263

Query: 181 KGENWEAKDMMDLLMEVRDEDGEGFDDETITEIIFGMLFGGQETTAFTTMWAVLFLTDNP 240
           +G       ++ +L+  +D+   G  D  I + I G++F   +TTA    W + +L D+P
Sbjct: 264 EG------GLLGVLLGAKDQKRNGLSDSQIADNIIGVIFAATDTTASVLTWLLKYLHDHP 323

Query: 241 HIFQKAKEEQEDIIRRRASTQKGVSLSEIKQMKFLSQVI 279
           ++ Q+   EQ  I ++     + +S  + ++M   ++VI
Sbjct: 324 NLLQEVSREQFSIRQKIKKENRRISWEDTRKMPLTTRVI 353

[Close]

BLAST of CSPI03G01900 vs. TAIR10
Match: AT5G45340.1 (AT5G45340.1 cytochrome P450, family 707, subfamily A, polypeptide 3)

HSP 1 Score: 109.0 bits (271), Expect = 4.8e-24
Identity = 76/279 (27.24%), Postives = 131/279 (46.95%), Query Frame = 1


Query: 1   MYKTHIFGKPTIIVCKPEICRQILTDETK-FIPSYPTSITAVFGKKSLLQVPKEEHRKLR 60
           ++KTH+ G P +++  PE  + +L  ++  F P++P S   + GK+++     + H KLR
Sbjct: 71  VFKTHVLGCPCVMISSPEAAKFVLVTKSHLFKPTFPASKERMLGKQAIFFHQGDYHSKLR 130

Query: 61  RLTMAPISGHAALEMYIDHIEHTVISGLEEWSSMKKPLELLTTIKQLTFKVIWNIFMGST 120
           +L +      A   M + HIE      L  W   +  L     +K  TF V     +G  
Sbjct: 131 KLVLRAFMPDAIRNM-VPHIESIAQESLNSWDGTQ--LNTYQEMKTYTFNVALISILGKD 190

Query: 121 PIKSTSIGEMETLNENIALAFLTLPLNFPGLPFHKALMAGKRLNEILQSYLDEKRMVKKS 180
            +      +++     +   + ++P+N PG  FHKA+ A K L +IL + L ++R    S
Sbjct: 191 EVYYRE--DLKRCYYILEKGYNSMPINLPGTLFHKAMKARKELAQILANILSKRRQNPSS 250

Query: 181 KGENWEAKDMMDLLMEVRDEDGEGFDDETITEIIFGMLFGGQETTAFTTMWAVLFLTDNP 240
                   D++   ME    D  G  DE I + I G++F  ++TTA    W + +L DNP
Sbjct: 251 H------TDLLGSFME----DKAGLTDEQIADNIIGVIFAARDTTASVLTWILKYLADNP 310

Query: 241 HIFQKAKEEQEDIIRRRASTQKGVSLSEIKQMKFLSQVI 279
            + +   EEQ   IR+     + ++  + K+M    +VI
Sbjct: 311 TVLEAVTEEQM-AIRKDKKEGESLTWEDTKKMPLTYRVI 333

[Close]

BLAST of CSPI03G01900 vs. TAIR10
Match: AT1G19630.1 (AT1G19630.1 cytochrome P450, family 722, subfamily A, polypeptide 1)

HSP 1 Score: 107.8 bits (268), Expect = 1.1e-23
Identity = 76/278 (27.34%), Postives = 134/278 (48.20%), Query Frame = 1


Query: 2   YKTHIFGKPTIIVCKPEICRQILTDET-KFIPSYPTSITAVFGKKSLLQVPKEEHRKLRR 61
           ++T +FG+  + +   E  R +L +++  F   Y  SI  + G +SLL  P + H K+ R
Sbjct: 76  FRTSLFGETHVFLSTTESARLVLNNDSGMFTKRYIKSIGELVGDRSLLCAP-QHHHKILR 135

Query: 62  LTMAPISGHAALEMYIDHIEHTVISGLEEWSSMKKPLELLTTIKQLTFKVIWNIFMGSTP 121
             +  +    +  + + H +  V+  L  W   +  + LLT + Q+TFK +  + +    
Sbjct: 136 SRLINLFSKRSTALMVRHFDELVVDALGGWEH-RGTVVLLTDLLQITFKAMCKMLVSLE- 195

Query: 122 IKSTSIGEMETLNENIALAFLTLPLNFPGLPFHKALMAGKRLNEILQSYLDEKRMVKKSK 181
            K   +G M+     +  A L  PLN P   FHK +MA  R+ E+L+  + E+R   +  
Sbjct: 196 -KEEELGSMQRDVGFVCEAMLAFPLNLPWTRFHKGIMARGRVMEMLEKIIRERR--NEIN 255

Query: 182 GENWEAKDMMDLLMEVRDEDGEGFDDETITEIIFGMLFGGQETTAFTTMWAVLFLTDNPH 241
             N   +D +  L+ V D D     D  I + I  M+  GQ+TTA    W V +L +N  
Sbjct: 256 SHNNHHEDFLQQLLAV-DNDTPQLTDAEIKDNILTMIIAGQDTTASALTWMVKYLGENQK 315

Query: 242 IFQKAKEEQEDIIRRRASTQKGVSLSEIKQMKFLSQVI 279
           +     EEQ   I ++AS +  + L ++ +M + S+++
Sbjct: 316 VLDILIEEQSQ-ITKKASNKPFLELEDLSEMPYASKMV 345

[Close]

BLAST of CSPI03G01900 vs. NCBI nr
Match: gi|659095691|ref|XP_008448717.1| (PREDICTED: beta-amyrin 11-oxidase-like isoform X1 [Cucumis melo])

HSP 1 Score: 495.7 bits (1275), Expect = 5.2e-137
Identity = 247/280 (88.21%), Postives = 261/280 (93.21%), Query Frame = 1


Query: 1   MYKTHIFGKPTIIVCKPEICRQI-LTDETKFIPSYPTSITAVFGKKSLLQVPKEEHRKLR 60
           MYKTHIFGKPTIIVCKPEICRQ+ LT+E KFIP YP ++T +FG+KSL QVPKEEHRKLR
Sbjct: 84  MYKTHIFGKPTIIVCKPEICRQVLLTEEAKFIPGYPITMTTIFGRKSLHQVPKEEHRKLR 143

Query: 61  RLTMAPISGHAALEMYIDHIEHTVISGLEEWSSMKKPLELLTTIKQLTFKVIWNIFMGST 120
           RLT APISGHAALEMYIDHIEHTVISGLEEWS  +KPLELLTTIK LT KVIWNIFMGST
Sbjct: 144 RLTTAPISGHAALEMYIDHIEHTVISGLEEWSRTEKPLELLTTIKHLTLKVIWNIFMGST 203

Query: 121 PIKSTSIGEMETLNENIALAFLTLPLNFPGLPFHKALMAGKRLNEILQSYLDEKRMVKKS 180
           PIKSTSI EME LNE+IALAF TLP+NFPG PFHKALM  KRLNEILQS LDEKRMVKKS
Sbjct: 204 PIKSTSIREMENLNEDIALAFFTLPINFPGFPFHKALMGRKRLNEILQSILDEKRMVKKS 263

Query: 181 KGENWEAKDMMDLLMEVRDEDGEGFDDETITEIIFGMLFGGQETTAFTTMWAVLFLTDNP 240
           KGENW+AKDMMDLLMEVRDEDGEGFDDETITE+IF MLFGGQET+AFTTMWAVLFLTDNP
Sbjct: 264 KGENWKAKDMMDLLMEVRDEDGEGFDDETITEMIFSMLFGGQETSAFTTMWAVLFLTDNP 323

Query: 241 HIFQKAKEEQEDIIRRRASTQKGVSLSEIKQMKFLSQVID 280
           HIFQKAKEEQEDI+RRRASTQKGVSLSEIKQMK+LSQVID
Sbjct: 324 HIFQKAKEEQEDIVRRRASTQKGVSLSEIKQMKYLSQVID 363

[Close]

BLAST of CSPI03G01900 vs. NCBI nr
Match: gi|700200656|gb|KGN55789.1| (Cytochrome P450 88D6 [Cucumis sativus])

HSP 1 Score: 481.5 bits (1238), Expect = 1.0e-132
Identity = 239/279 (85.66%), Postives = 255/279 (91.40%), Query Frame = 1


Query: 1   MYKTHIFGKPTIIVCKPEICRQILTDETKFIPSYPTSITAVFGKKSLLQVPKEEHRKLRR 60
           MYKTHIFGKPTIIVCKPE+CRQ+LTDE KFIP YPT++  +FGKKSL  V KEEHRKLRR
Sbjct: 1   MYKTHIFGKPTIIVCKPELCRQVLTDEAKFIPGYPTTMKTLFGKKSLHAVTKEEHRKLRR 60

Query: 61  LTMAPISGHAALEMYIDHIEHTVISGLEEWSSMKKPLELLTTIKQLTFKVIWNIFMGSTP 120
           L   PIS HAALEMYIDHIE TVISGLEEWSSM+KPLELLTTIKQLTFKVIWNIFMGSTP
Sbjct: 61  LITTPISSHAALEMYIDHIERTVISGLEEWSSMEKPLELLTTIKQLTFKVIWNIFMGSTP 120

Query: 121 IKSTSIGEMETLNENIALAFLTLPLNFPGLPFHKALMAGKRLNEILQSYLDEKRMVKKSK 180
           IKSTSI EMET N++I L F T+P+NFPG  FHKAL+A K L+EILQS LDEKRMVKKSK
Sbjct: 121 IKSTSIREMETFNDDIVLGFFTMPINFPGFSFHKALLARKGLHEILQSILDEKRMVKKSK 180

Query: 181 GENWEAKDMMDLLMEVRDEDGEGFDDETITEIIFGMLFGGQETTAFTTMWAVLFLTDNPH 240
           GENWEAKDMMDLLMEVRDEDGEGFDDETITE+IF MLFGGQET+AFT+MWAVLFLTDNPH
Sbjct: 181 GENWEAKDMMDLLMEVRDEDGEGFDDETITEMIFSMLFGGQETSAFTSMWAVLFLTDNPH 240

Query: 241 IFQKAKEEQEDIIRRRASTQKGVSLSEIKQMKFLSQVID 280
           IFQKAKEEQEDII+RRASTQ GVSLSEIKQMKFLSQVID
Sbjct: 241 IFQKAKEEQEDIIKRRASTQTGVSLSEIKQMKFLSQVID 279

[Close]

BLAST of CSPI03G01900 vs. NCBI nr
Match: gi|659095697|ref|XP_008448720.1| (PREDICTED: beta-amyrin 11-oxidase-like isoform X1 [Cucumis melo])

HSP 1 Score: 469.5 bits (1207), Expect = 4.0e-129
Identity = 231/279 (82.80%), Postives = 255/279 (91.40%), Query Frame = 1


Query: 1   MYKTHIFGKPTIIVCKPEICRQILTDETKFIPSYPTSITAVFGKKSLLQVPKEEHRKLRR 60
           MYKTHIFG  TIIVCKPEICRQ+LTDE+KFIP+Y T++  +FGK+SL QV KEEH+KLRR
Sbjct: 84  MYKTHIFGNLTIIVCKPEICRQVLTDESKFIPAYHTTMKTLFGKRSLHQVSKEEHQKLRR 143

Query: 61  LTMAPISGHAALEMYIDHIEHTVISGLEEWSSMKKPLELLTTIKQLTFKVIWNIFMGSTP 120
           L   PISGHA+LEMYIDHIE T ISG EEWSSM+KPLELLT+IKQLTFKVIWNIFMGSTP
Sbjct: 144 LITIPISGHASLEMYIDHIEQTAISGFEEWSSMEKPLELLTSIKQLTFKVIWNIFMGSTP 203

Query: 121 IKSTSIGEMETLNENIALAFLTLPLNFPGLPFHKALMAGKRLNEILQSYLDEKRMVKKSK 180
            KST+I EME+LN++I LAF T+P+NFPG  FHKAL A KRL+EILQS LDEK+MVKK K
Sbjct: 204 TKSTTIREMESLNDDIVLAFFTMPINFPGFSFHKALKARKRLHEILQSILDEKKMVKKRK 263

Query: 181 GENWEAKDMMDLLMEVRDEDGEGFDDETITEIIFGMLFGGQETTAFTTMWAVLFLTDNPH 240
           GE+WEAKDMMDLLMEVRDEDGEGFDDETITE+IFGMLFGGQET+AFTTMWA+LFLTDNPH
Sbjct: 264 GESWEAKDMMDLLMEVRDEDGEGFDDETITEMIFGMLFGGQETSAFTTMWAILFLTDNPH 323

Query: 241 IFQKAKEEQEDIIRRRASTQKGVSLSEIKQMKFLSQVID 280
           IFQKAKEEQEDIIRRR STQKG+SLSEIKQMKFLSQVID
Sbjct: 324 IFQKAKEEQEDIIRRRVSTQKGISLSEIKQMKFLSQVID 362

[Close]

BLAST of CSPI03G01900 vs. NCBI nr
Match: gi|659095699|ref|XP_008448721.1| (PREDICTED: beta-amyrin 11-oxidase-like isoform X2 [Cucumis melo])

HSP 1 Score: 465.3 bits (1196), Expect = 7.5e-128
Identity = 229/277 (82.67%), Postives = 253/277 (91.34%), Query Frame = 1


Query: 1   MYKTHIFGKPTIIVCKPEICRQILTDETKFIPSYPTSITAVFGKKSLLQVPKEEHRKLRR 60
           MYKTHIFG  TIIVCKPEICRQ+LTDE+KFIP+Y T++  +FGK+SL QV KEEH+KLRR
Sbjct: 84  MYKTHIFGNLTIIVCKPEICRQVLTDESKFIPAYHTTMKTLFGKRSLHQVSKEEHQKLRR 143

Query: 61  LTMAPISGHAALEMYIDHIEHTVISGLEEWSSMKKPLELLTTIKQLTFKVIWNIFMGSTP 120
           L   PISGHA+LEMYIDHIE T ISG EEWSSM+KPLELLT+IKQLTFKVIWNIFMGSTP
Sbjct: 144 LITIPISGHASLEMYIDHIEQTAISGFEEWSSMEKPLELLTSIKQLTFKVIWNIFMGSTP 203

Query: 121 IKSTSIGEMETLNENIALAFLTLPLNFPGLPFHKALMAGKRLNEILQSYLDEKRMVKKSK 180
            KST+I EME+LN++I LAF T+P+NFPG  FHKAL A KRL+EILQS LDEK+MVKK K
Sbjct: 204 TKSTTIREMESLNDDIVLAFFTMPINFPGFSFHKALKARKRLHEILQSILDEKKMVKKRK 263

Query: 181 GENWEAKDMMDLLMEVRDEDGEGFDDETITEIIFGMLFGGQETTAFTTMWAVLFLTDNPH 240
           GE+WEAKDMMDLLMEVRDEDGEGFDDETITE+IFGMLFGGQET+AFTTMWA+LFLTDNPH
Sbjct: 264 GESWEAKDMMDLLMEVRDEDGEGFDDETITEMIFGMLFGGQETSAFTTMWAILFLTDNPH 323

Query: 241 IFQKAKEEQEDIIRRRASTQKGVSLSEIKQMKFLSQV 278
           IFQKAKEEQEDIIRRR STQKG+SLSEIKQMKFLSQV
Sbjct: 324 IFQKAKEEQEDIIRRRVSTQKGISLSEIKQMKFLSQV 360

[Close]

BLAST of CSPI03G01900 vs. NCBI nr
Match: gi|659095693|ref|XP_008448718.1| (PREDICTED: beta-amyrin 11-oxidase-like isoform X2 [Cucumis melo])

HSP 1 Score: 435.6 bits (1119), Expect = 6.3e-119
Identity = 218/246 (88.62%), Postives = 230/246 (93.50%), Query Frame = 1


Query: 34  YPTSITAVFGKKSLLQVPKEEHRKLRRLTMAPISGHAALEMYIDHIEHTVISGLEEWSSM 93
           YP ++T +FG+KSL QVPKEEHRKLRRLT APISGHAALEMYIDHIEHTVISGLEEWS  
Sbjct: 39  YPITMTTIFGRKSLHQVPKEEHRKLRRLTTAPISGHAALEMYIDHIEHTVISGLEEWSRT 98

Query: 94  KKPLELLTTIKQLTFKVIWNIFMGSTPIKSTSIGEMETLNENIALAFLTLPLNFPGLPFH 153
           +KPLELLTTIK LT KVIWNIFMGSTPIKSTSI EME LNE+IALAF TLP+NFPG PFH
Sbjct: 99  EKPLELLTTIKHLTLKVIWNIFMGSTPIKSTSIREMENLNEDIALAFFTLPINFPGFPFH 158

Query: 154 KALMAGKRLNEILQSYLDEKRMVKKSKGENWEAKDMMDLLMEVRDEDGEGFDDETITEII 213
           KALM  KRLNEILQS LDEKRMVKKSKGENW+AKDMMDLLMEVRDEDGEGFDDETITE+I
Sbjct: 159 KALMGRKRLNEILQSILDEKRMVKKSKGENWKAKDMMDLLMEVRDEDGEGFDDETITEMI 218

Query: 214 FGMLFGGQETTAFTTMWAVLFLTDNPHIFQKAKEEQEDIIRRRASTQKGVSLSEIKQMKF 273
           F MLFGGQET+AFTTMWAVLFLTDNPHIFQKAKEEQEDI+RRRASTQKGVSLSEIKQMK+
Sbjct: 219 FSMLFGGQETSAFTTMWAVLFLTDNPHIFQKAKEEQEDIVRRRASTQKGVSLSEIKQMKY 278

Query: 274 LSQVID 280
           LSQVID
Sbjct: 279 LSQVID 284

The following BLAST results are available for this feature:

BLAST of CSPI03G01900 vs. Swiss-Prot


Match Name	E-value	Identity	Description
BAMO_GLYUR	7.9e-69	46.79	Beta-amyrin 11-oxidase OS=Glycyrrhiza uralensis GN=CYP88D6 PE=1 SV=1	[more]
KAO1_ARATH	2.0e-56	41.94	Ent-kaurenoic acid oxidase 1 OS=Arabidopsis thaliana GN=KAO1 PE=2 SV=1	[more]
KAO2_ARATH	4.6e-53	41.58	Ent-kaurenoic acid oxidase 2 OS=Arabidopsis thaliana GN=KAO2 PE=2 SV=2	[more]
C88A1_MAIZE	8.7e-52	37.99	Cytochrome P450 88A1 OS=Zea mays GN=CYP88A1 PE=2 SV=1	[more]
KAO1_HORVU	3.4e-48	37.23	Ent-kaurenoic acid oxidase 1 OS=Hordeum vulgare GN=KAO1 PE=1 SV=1	[more]

BLAST of CSPI03G01900 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L419_CUCSA	7.0e-133	85.66	Cytochrome P450 88D6 OS=Cucumis sativus GN=Csa_3G015360 PE=4 SV=1	[more]
A0A0A0L1B5_CUCSA	1.8e-104	68.82	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G011860 PE=3 SV=1	[more]
A0A0A0L415_CUCSA	4.8e-97	64.64	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G011850 PE=4 SV=1	[more]
A0A0A0L1Y8_CUCSA	3.9e-83	95.06	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G014360 PE=4 SV=1	[more]
A0A061G3A5_THECC	1.4e-67	48.75	Cytochrome P450 88D6, putative OS=Theobroma cacao GN=TCM_015758 PE=3 SV=1	[more]

BLAST of CSPI03G01900 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G05160.1	1.1e-57	41.94	cytochrome P450, family 88, subfamily A, polypeptide 3	[more]
AT2G32440.1	2.6e-54	41.58	ent-kaurenoic acid hydroxylase 2	[more]
AT2G29090.1	3.6e-27	26.88	cytochrome P450, family 707, subfamily A, polypeptide 2	[more]
AT5G45340.1	4.8e-24	27.24	cytochrome P450, family 707, subfamily A, polypeptide 3	[more]
AT1G19630.1	1.1e-23	27.34	cytochrome P450, family 722, subfamily A, polypeptide 1	[more]

BLAST of CSPI03G01900 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659095691\|ref\|XP_008448717.1\|	5.2e-137	88.21	PREDICTED: beta-amyrin 11-oxidase-like isoform X1 [Cucumis melo]	[more]
gi\|700200656\|gb\|KGN55789.1\|	1.0e-132	85.66	Cytochrome P450 88D6 [Cucumis sativus]	[more]
gi\|659095697\|ref\|XP_008448720.1\|	4.0e-129	82.80	PREDICTED: beta-amyrin 11-oxidase-like isoform X1 [Cucumis melo]	[more]
gi\|659095699\|ref\|XP_008448721.1\|	7.5e-128	82.67	PREDICTED: beta-amyrin 11-oxidase-like isoform X2 [Cucumis melo]	[more]
gi\|659095693\|ref\|XP_008448718.1\|	6.3e-119	88.62	PREDICTED: beta-amyrin 11-oxidase-like isoform X2 [Cucumis melo]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR001128	Cyt_P450

Vocabulary: **Molecular Function**
Term	Definition
GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
GO:0005506	iron ion binding
GO:0020037	heme binding

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	oxidation-reduction process

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055114	oxidation-reduction process
cellular_component	GO:0005575	cellular_component
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G01900.1	CSPI03G01900.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	GENE3D	G3DSA:1.10.630.10		coord: 2..278 score: 7.4
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 2..278 score: 1.0
IPR001128	Cytochrome P450	unknown	SSF48264	Cytochrome P450	coord: 2..278 score: 1.23
None	No IPR available	PANTHER	PTHR24286	FAMILY NOT NAMED	coord: 1..279 score: 1.6E
None	No IPR available	PANTHER	PTHR24286:SF67	ENT-KAURENOIC ACID OXIDASE 1-RELATED	coord: 1..279 score: 1.6E

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CSPI03G01900	Csa3G011860	Cucumber (Chinese Long) v2	cpicuB120
CSPI03G01900	MELO3C003387.2	Melon (DHL92) v3.6.1	cpimedB213
CSPI03G01900	CsaV3_3G001930	Cucumber (Chinese Long) v3	cpicucB144
CSPI03G01900	Cucsa.313370	Cucumber (Gy14) v1	cgycpiB474
CSPI03G01900	CmaCh02G016010	Cucurbita maxima (Rimu)	cmacpiB608
CSPI03G01900	CsGy3G044340	Cucumber (Gy14) v2	cgybcpiB113
CSPI03G01900	Carg23223	Silver-seed gourd	carcpiB0742

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CSPI03G01900	Cucurbita pepo (Zucchini)	cpecpiB207
CSPI03G01900	Cucurbita pepo (Zucchini)	cpecpiB720
CSPI03G01900	Bottle gourd (USVL1VR-Ls)	cpilsiB218
CSPI03G01900	Bottle gourd (USVL1VR-Ls)	cpilsiB227
CSPI03G01900	Melon (DHL92) v3.6.1	cpimedB225
CSPI03G01900	Cucumber (Gy14) v2	cgybcpiB110
CSPI03G01900	Silver-seed gourd	carcpiB0597
CSPI03G01900	Silver-seed gourd	carcpiB0818
CSPI03G01900	Cucumber (Chinese Long) v3	cpicucB146
CSPI03G01900	Watermelon (97103) v2	cpiwmbB184
CSPI03G01900	Watermelon (97103) v2	cpiwmbB261
CSPI03G01900	Wax gourd	cpiwgoB239
CSPI03G01900	Wild cucumber (PI 183967)	cpicpiB100
CSPI03G01900	Wild cucumber (PI 183967)	cpicpiB103
CSPI03G01900	Cucumber (Gy14) v1	cgycpiB484
CSPI03G01900	Cucurbita maxima (Rimu)	cmacpiB262
CSPI03G01900	Cucurbita maxima (Rimu)	cmacpiB325
CSPI03G01900	Cucurbita maxima (Rimu)	cmacpiB838
CSPI03G01900	Cucurbita moschata (Rifu)	cmocpiB316
CSPI03G01900	Cucurbita moschata (Rifu)	cmocpiB597
CSPI03G01900	Cucurbita moschata (Rifu)	cmocpiB823
CSPI03G01900	Cucumber (Chinese Long) v2	cpicuB122
CSPI03G01900	Cucumber (Chinese Long) v2	cpicuB125
CSPI03G01900	Melon (DHL92) v3.5.1	cpimeB218
CSPI03G01900	Melon (DHL92) v3.5.1	cpimeB238
CSPI03G01900	Melon (DHL92) v3.5.1	cpimeB240
CSPI03G01900	Watermelon (Charleston Gray)	cpiwcgB180
CSPI03G01900	Watermelon (Charleston Gray)	cpiwcgB190
CSPI03G01900	Watermelon (Charleston Gray)	cpiwcgB235
CSPI03G01900	Watermelon (97103) v1	cpiwmB184
CSPI03G01900	Watermelon (97103) v1	cpiwmB200
CSPI03G01900	Watermelon (97103) v1	cpiwmB240