CSPI03G00500 (gene) Wild cucumber (PI 183967) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CSPI03G00500
Type	gene
Organism	Cucumis sativus (Wild cucumber (PI 183967))
Description	Aconitate hydratase
Location	Chr3 : 370511 .. 371282 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCAGAGCTTTGCTAGACCAAAGGAGTCACAGACTAAGGTTGTAGAGTTCAGTTTTCATGGAACAACAGCTGAGCTTAGGCATGGTGATGTTGTAATAGCAGCGATTGCTAGCTGCACCAATACCTCCAATCCTAGTATAAGGCTTGGGGCTGCTTTGGTTGCCAAGAAAGCTTGTGAACTTAGTTTAGAGGTGTGAAAGTTCTACCTCCTCACATTGCCTGTGCATCAATAATTTATTTCAGAAGGACAGTGACGAACAAAATGAAACATTTTATAAATTATATTCCCAAACACACCATTAATATTTGGCTTTCTAAAGAGGTTAAGCCATGGATAAACACCGGTCTTGCTCCAGGTGCAAGAGTGGTAACCAAATACTTGGAAAAGAGGTGCCACTATCAAACTCTCCAAATGCTTCTGTTGTTAAATGAGAAATAAAAATTGAATAAAAACACTCAACAACGAATGAACTAATATAGTTCCATTATTCTTCTGACTGGGAGTTTTTCTGTTTCTCTTCTTGTTGTTGCTGATGTTGTGTGGAGGGTAATATTTCTCAAGTCAAGAGTTGGATTTCTGCAGTGAATTGTAGAAGTATTTAGATAAGCTGGGGTTCAATACTGTCGGGGAGTATGGATGCACAACATACATTGGCAATTCAGGGGATTTAGATGAAGAAGTGGCATCTGCAATTTCTGACGACGAATAGAAAAGTTTAATTATTACCACTTGTACCCTCTAGCCTCTCTAGCTCAGGCAAGCAATTGAAT

mRNA sequence

ATGCAGAGCTTTGCTAGACCAAAGGAGTCACAGACTAAGGTTGTAGAGTTCAGTTTTCATGGAACAACAGCTGAGCTTAGGCATGGTGATGTTGTAATAGCAGCGATTGCTAGCTGCACCAATACCTCCAATCCTAGTATAAGGCTTGGGGCTGCTTTGGTTGCCAAGAAAGCTTGTGAACTTAGTTTAGAGGTTAAGCCATGGATAAACACCGGTCTTGCTCCAGGTGCAAGAGTGGTAACCAAATACTTGGAAAAGAGTTCCATTATTCTTCTGACTGGGAGTTTTTCTGTTTCTCTTCTTGTTGTTGCTGATGTTGTGTGGAGGAAGTATTTAGATAAGCTGGGGTTCAATACTGTCGGGGAGTATGGATGCACAACATACATTGGCAATTCAGGGGATTTAGATGAAGAAGTGGCATCTGCAATTTCTGACGACGAATAG

Coding sequence (CDS)

ATGCAGAGCTTTGCTAGACCAAAGGAGTCACAGACTAAGGTTGTAGAGTTCAGTTTTCATGGAACAACAGCTGAGCTTAGGCATGGTGATGTTGTAATAGCAGCGATTGCTAGCTGCACCAATACCTCCAATCCTAGTATAAGGCTTGGGGCTGCTTTGGTTGCCAAGAAAGCTTGTGAACTTAGTTTAGAGGTTAAGCCATGGATAAACACCGGTCTTGCTCCAGGTGCAAGAGTGGTAACCAAATACTTGGAAAAGAGTTCCATTATTCTTCTGACTGGGAGTTTTTCTGTTTCTCTTCTTGTTGTTGCTGATGTTGTGTGGAGGAAGTATTTAGATAAGCTGGGGTTCAATACTGTCGGGGAGTATGGATGCACAACATACATTGGCAATTCAGGGGATTTAGATGAAGAAGTGGCATCTGCAATTTCTGACGACGAATAG

Homology

[Close]

BLAST of CSPI03G00500 vs. Swiss-Prot
Match: ACO1_ARATH (Aconitate hydratase 1 OS=Arabidopsis thaliana GN=ACO1 PE=1 SV=2)

HSP 1 Score: 175.6 bits (444), Expect = 3.9e-43
Identity = 93/146 (63.70%), Postives = 110/146 (75.34%), Query Frame = 1


Query: 2   QSFARPKESQTKVVEFSFHGTTAELRHGDVVIAAIASCTNTSNPSIRLGAALVAKKACEL 61
           + FA PKE+Q+K VEF+F+GTTA+LRHGDVVIAAI SCTNTSNPS+ LGAALVAKKAC+L
Sbjct: 404 KGFAVPKEAQSKAVEFNFNGTTAQLRHGDVVIAAITSCTNTSNPSVMLGAALVAKKACDL 463

Query: 62  SLEVKPWINTGLAPGARVVTKYLEKSSIILLTGSFSVSLLVVADVVWRKYLDKLGFNTVG 121
            LEVKPWI T LAPG+ VVTKYL KS +                   +KYL++LGF+ VG
Sbjct: 464 GLEVKPWIKTSLAPGSGVVTKYLAKSGL-------------------QKYLNQLGFSIVG 523

Query: 122 EYGCTTYIGNSGDLDEEVASAISDDE 148
            YGCTT IGNSGD+ E VASAI D++
Sbjct: 524 -YGCTTCIGNSGDIHEAVASAIVDND 529

[Close]

BLAST of CSPI03G00500 vs. Swiss-Prot
Match: ACOC_ORYSJ (Putative aconitate hydratase, cytoplasmic OS=Oryza sativa subsp. japonica GN=Os08g0191100 PE=3 SV=1)

HSP 1 Score: 168.7 bits (426), Expect = 4.8e-41
Identity = 89/146 (60.96%), Postives = 106/146 (72.60%), Query Frame = 1


Query: 2   QSFARPKESQTKVVEFSFHGTTAELRHGDVVIAAIASCTNTSNPSIRLGAALVAKKACEL 61
           + FA PKE Q KVV+F FHG  AEL+HG VVIAAI SCTNTSNPS+ LGAALVAKKACEL
Sbjct: 404 KGFAVPKEQQDKVVKFDFHGQPAELKHGSVVIAAITSCTNTSNPSVMLGAALVAKKACEL 463

Query: 62  SLEVKPWINTGLAPGARVVTKYLEKSSIILLTGSFSVSLLVVADVVWRKYLDKLGFNTVG 121
            LEVKPW+ T LAPG+ VVTKYL +S +                   ++YL+K GF+ VG
Sbjct: 464 GLEVKPWVKTSLAPGSGVVTKYLLQSGL-------------------QEYLNKQGFHVVG 523

Query: 122 EYGCTTYIGNSGDLDEEVASAISDDE 148
            YGCTT IGNSGDLDE V++AIS+++
Sbjct: 524 -YGCTTCIGNSGDLDESVSAAISEND 529

[Close]

BLAST of CSPI03G00500 vs. Swiss-Prot
Match: ACOC_SOLTU (Aconitate hydratase, cytoplasmic (Fragment) OS=Solanum tuberosum PE=2 SV=1)

HSP 1 Score: 167.5 bits (423), Expect = 1.1e-40
Identity = 91/146 (62.33%), Postives = 104/146 (71.23%), Query Frame = 1


Query: 2   QSFARPKESQTKVVEFSFHGTTAELRHGDVVIAAIASCTNTSNPSIRLGAALVAKKACEL 61
           + FA PKE Q KV +FSFHG  AEL+HG VVIAAI SCTNTSNPS+ LGAALVAKKA EL
Sbjct: 122 KGFAVPKEVQDKVAKFSFHGQPAELKHGSVVIAAITSCTNTSNPSVMLGAALVAKKASEL 181

Query: 62  SLEVKPWINTGLAPGARVVTKYLEKSSIILLTGSFSVSLLVVADVVWRKYLDKLGFNTVG 121
            L VKPW+ T LAPG+ VVTKYL KS +                   +KYL++ GFN VG
Sbjct: 182 GLHVKPWVKTSLAPGSGVVTKYLLKSGL-------------------QKYLNQQGFNIVG 241

Query: 122 EYGCTTYIGNSGDLDEEVASAISDDE 148
            YGCTT IGNSGDLDE VASAIS+++
Sbjct: 242 -YGCTTCIGNSGDLDESVASAISEND 247

[Close]

BLAST of CSPI03G00500 vs. Swiss-Prot
Match: ACOC_CUCMA (Aconitate hydratase, cytoplasmic OS=Cucurbita maxima PE=2 SV=1)

HSP 1 Score: 166.4 bits (420), Expect = 2.4e-40
Identity = 88/146 (60.27%), Postives = 105/146 (71.92%), Query Frame = 1


Query: 2   QSFARPKESQTKVVEFSFHGTTAELRHGDVVIAAIASCTNTSNPSIRLGAALVAKKACEL 61
           + FA PKE+Q  V +FSFHG  AEL+HG VVIAAI SCTNTSNPS+ LGAALVAKKACEL
Sbjct: 404 KGFAIPKEAQENVAKFSFHGQPAELKHGSVVIAAITSCTNTSNPSVMLGAALVAKKACEL 463

Query: 62  SLEVKPWINTGLAPGARVVTKYLEKSSIILLTGSFSVSLLVVADVVWRKYLDKLGFNTVG 121
            L+VKPW+ T LAPG+ VVTKYL KS +                   + YL++ GF+ VG
Sbjct: 464 GLQVKPWVKTSLAPGSGVVTKYLLKSGL-------------------QPYLNQQGFHIVG 523

Query: 122 EYGCTTYIGNSGDLDEEVASAISDDE 148
            YGCTT IGNSGDLDE V++AISD++
Sbjct: 524 -YGCTTCIGNSGDLDESVSAAISDND 529

[Close]

BLAST of CSPI03G00500 vs. Swiss-Prot
Match: ACOC_CUCMC (Aconitate hydratase (Fragment) OS=Cucumis melo var. conomon GN=ACO PE=2 SV=1)

HSP 1 Score: 160.2 bits (404), Expect = 1.7e-38
Identity = 89/146 (60.96%), Postives = 104/146 (71.23%), Query Frame = 1


Query: 2   QSFARPKESQTKVVEFSFHGTTAELRHGDVVIAAIASCTNTSNPSIRLGAALVAKKACEL 61
           + FA PKE+Q KV EF+FHG+ A+LRHGDVVIAAI SCTNTS+ S+ LGAALVAKKACEL
Sbjct: 270 KGFAIPKEAQVKVAEFNFHGSPAQLRHGDVVIAAITSCTNTSS-SVMLGAALVAKKACEL 329

Query: 62  SLEVKPWINTGLAPGARVVTKYLEKSSIILLTGSFSVSLLVVADVVWRKYLDKLGFNTVG 121
            LEVKPWI T L     VVTKYL KS +                   +KYL++LGFN VG
Sbjct: 330 GLEVKPWIKTVLLQALGVVTKYLAKSGL-------------------QKYLNQLGFNIVG 389

Query: 122 EYGCTTYIGNSGDLDEEVASAISDDE 148
            YGCTT IGNSGD+DE VASAI+ ++
Sbjct: 390 -YGCTTCIGNSGDIDESVASAITGND 394

[Close]

BLAST of CSPI03G00500 vs. TrEMBL
Match: A0A0A0L3M2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G002540 PE=4 SV=1)

HSP 1 Score: 274.2 bits (700), Expect = 9.0e-71
Identity = 143/156 (91.67%), Postives = 145/156 (92.95%), Query Frame = 1


Query: 2   QSFARPKESQTKVVEFSFHGTTAELRHGDVVIAAIASCTNTSNPSIRLGAALVAKKACEL 61
           +SFARPKESQTKVVEFSFHGTTAELRHGDVVIAAIASCTNTSNPSIRLGAALVAKK CEL
Sbjct: 29  KSFARPKESQTKVVEFSFHGTTAELRHGDVVIAAIASCTNTSNPSIRLGAALVAKKVCEL 88

Query: 62  SLEV----------KPWINTGLAPGARVVTKYLEKSSIILLTGSFSVSLLVVADVVWRKY 121
           SLEV          KPWINTGLAPG+RVVTKYLEKSSIILLTGSFSVSLLVVADVVWRKY
Sbjct: 89  SLEVCKFYLLTLPVKPWINTGLAPGSRVVTKYLEKSSIILLTGSFSVSLLVVADVVWRKY 148

Query: 122 LDKLGFNTVGEYGCTTYIGNSGDLDEEVASAISDDE 148
           LDKLGFNTVGEYGCTTYIGNSGDLDEEVASAISDDE
Sbjct: 149 LDKLGFNTVGEYGCTTYIGNSGDLDEEVASAISDDE 184

[Close]

BLAST of CSPI03G00500 vs. TrEMBL
Match: M0RSV3_MUSAM (Uncharacterized protein OS=Musa acuminata subsp. malaccensis PE=4 SV=1)

HSP 1 Score: 184.1 bits (466), Expect = 1.2e-43
Identity = 95/147 (64.63%), Postives = 111/147 (75.51%), Query Frame = 1


Query: 1   MQSFARPKESQTKVVEFSFHGTTAELRHGDVVIAAIASCTNTSNPSIRLGAALVAKKACE 60
           +  FA PKESQ K+ EFSFHGTT +++HGDVVIAAI SCTNTSNPS+ LGAALVAKKACE
Sbjct: 554 LHGFAVPKESQDKIAEFSFHGTTTQIKHGDVVIAAITSCTNTSNPSVMLGAALVAKKACE 613

Query: 61  LSLEVKPWINTGLAPGARVVTKYLEKSSIILLTGSFSVSLLVVADVVWRKYLDKLGFNTV 120
           L LEVKPWI T LAPG+ VVTKYLEKS +                   +KYL++LGFN V
Sbjct: 614 LGLEVKPWIKTSLAPGSGVVTKYLEKSGL-------------------QKYLNQLGFNIV 673

Query: 121 GEYGCTTYIGNSGDLDEEVASAISDDE 148
           G YGCTT IGNSGDLDE V++AIS+++
Sbjct: 674 G-YGCTTCIGNSGDLDEAVSAAISEND 680

[Close]

BLAST of CSPI03G00500 vs. TrEMBL
Match: A5C294_VITVI (Aconitate hydratase OS=Vitis vinifera GN=VITISV_003004 PE=3 SV=1)

HSP 1 Score: 184.1 bits (466), Expect = 1.2e-43
Identity = 97/146 (66.44%), Postives = 113/146 (77.40%), Query Frame = 1


Query: 2   QSFARPKESQTKVVEFSFHGTTAELRHGDVVIAAIASCTNTSNPSIRLGAALVAKKACEL 61
           + FA PKESQ+KVVEFS+HGT A+LRHGDVVIAAI SCTNTSNPS+ LGAALVAKKACEL
Sbjct: 357 KGFAIPKESQSKVVEFSYHGTPAQLRHGDVVIAAITSCTNTSNPSVMLGAALVAKKACEL 416

Query: 62  SLEVKPWINTGLAPGARVVTKYLEKSSIILLTGSFSVSLLVVADVVWRKYLDKLGFNTVG 121
            LEVKPWI T LAPG+ VVTKYLEKS +                   +KYL++LGF+ VG
Sbjct: 417 GLEVKPWIKTSLAPGSGVVTKYLEKSGL-------------------QKYLNQLGFHIVG 476

Query: 122 EYGCTTYIGNSGDLDEEVASAISDDE 148
            YGCTT IGNSGD++E VASAIS+++
Sbjct: 477 -YGCTTCIGNSGDINESVASAISEND 482

[Close]

BLAST of CSPI03G00500 vs. TrEMBL
Match: D7T1R6_VITVI (Aconitate hydratase OS=Vitis vinifera GN=VIT_00s0264g00030 PE=3 SV=1)

HSP 1 Score: 184.1 bits (466), Expect = 1.2e-43
Identity = 97/146 (66.44%), Postives = 113/146 (77.40%), Query Frame = 1


Query: 2   QSFARPKESQTKVVEFSFHGTTAELRHGDVVIAAIASCTNTSNPSIRLGAALVAKKACEL 61
           + FA PKESQ+KVVEFS+HGT A+LRHGDVVIAAI SCTNTSNPS+ LGAALVAKKACEL
Sbjct: 422 KGFAIPKESQSKVVEFSYHGTPAQLRHGDVVIAAITSCTNTSNPSVMLGAALVAKKACEL 481

Query: 62  SLEVKPWINTGLAPGARVVTKYLEKSSIILLTGSFSVSLLVVADVVWRKYLDKLGFNTVG 121
            LEVKPWI T LAPG+ VVTKYLEKS +                   +KYL++LGF+ VG
Sbjct: 482 GLEVKPWIKTSLAPGSGVVTKYLEKSGL-------------------QKYLNQLGFHIVG 541

Query: 122 EYGCTTYIGNSGDLDEEVASAISDDE 148
            YGCTT IGNSGD++E VASAIS+++
Sbjct: 542 -YGCTTCIGNSGDINESVASAISEND 547

[Close]

BLAST of CSPI03G00500 vs. TrEMBL
Match: B9MXB0_POPTR (Aconitate hydratase OS=Populus trichocarpa GN=POPTR_0005s10990g PE=3 SV=1)

HSP 1 Score: 183.7 bits (465), Expect = 1.6e-43
Identity = 97/146 (66.44%), Postives = 112/146 (76.71%), Query Frame = 1


Query: 2   QSFARPKESQTKVVEFSFHGTTAELRHGDVVIAAIASCTNTSNPSIRLGAALVAKKACEL 61
           + FA PKESQ+KV EFSF GT+A+LRHGDVVIAAI SCTNTSNPS+ LGAALVAKKACEL
Sbjct: 403 KGFAIPKESQSKVAEFSFRGTSAQLRHGDVVIAAITSCTNTSNPSVMLGAALVAKKACEL 462

Query: 62  SLEVKPWINTGLAPGARVVTKYLEKSSIILLTGSFSVSLLVVADVVWRKYLDKLGFNTVG 121
            LEVKPWI T LAPG+ VVTKYLEKS +                   +KYL++LGFN VG
Sbjct: 463 GLEVKPWIKTSLAPGSGVVTKYLEKSGL-------------------QKYLNQLGFNIVG 522

Query: 122 EYGCTTYIGNSGDLDEEVASAISDDE 148
            YGCTT IGNSGD+DE VASAI++++
Sbjct: 523 -YGCTTCIGNSGDIDEAVASAITEND 528

[Close]

BLAST of CSPI03G00500 vs. TAIR10
Match: AT4G35830.1 (AT4G35830.1 aconitase 1)

HSP 1 Score: 175.6 bits (444), Expect = 2.2e-44
Identity = 93/146 (63.70%), Postives = 110/146 (75.34%), Query Frame = 1


Query: 2   QSFARPKESQTKVVEFSFHGTTAELRHGDVVIAAIASCTNTSNPSIRLGAALVAKKACEL 61
           + FA PKE+Q+K VEF+F+GTTA+LRHGDVVIAAI SCTNTSNPS+ LGAALVAKKAC+L
Sbjct: 404 KGFAVPKEAQSKAVEFNFNGTTAQLRHGDVVIAAITSCTNTSNPSVMLGAALVAKKACDL 463

Query: 62  SLEVKPWINTGLAPGARVVTKYLEKSSIILLTGSFSVSLLVVADVVWRKYLDKLGFNTVG 121
            LEVKPWI T LAPG+ VVTKYL KS +                   +KYL++LGF+ VG
Sbjct: 464 GLEVKPWIKTSLAPGSGVVTKYLAKSGL-------------------QKYLNQLGFSIVG 523

Query: 122 EYGCTTYIGNSGDLDEEVASAISDDE 148
            YGCTT IGNSGD+ E VASAI D++
Sbjct: 524 -YGCTTCIGNSGDIHEAVASAIVDND 529

[Close]

BLAST of CSPI03G00500 vs. TAIR10
Match: AT2G05710.1 (AT2G05710.1 aconitase 3)

HSP 1 Score: 159.1 bits (401), Expect = 2.1e-39
Identity = 84/146 (57.53%), Postives = 104/146 (71.23%), Query Frame = 1


Query: 2   QSFARPKESQTKVVEFSFHGTTAELRHGDVVIAAIASCTNTSNPSIRLGAALVAKKACEL 61
           + FA PKE+Q KVV FSF G  AEL+HG VVIAAI SCTNTSNPS+ LGA LVAKKAC+L
Sbjct: 496 KGFAIPKEAQEKVVNFSFDGQPAELKHGSVVIAAITSCTNTSNPSVMLGAGLVAKKACDL 555

Query: 62  SLEVKPWINTGLAPGARVVTKYLEKSSIILLTGSFSVSLLVVADVVWRKYLDKLGFNTVG 121
            L+VKPWI T LAPG+ VVTKYL KS +                   ++YL++ GFN VG
Sbjct: 556 GLQVKPWIKTSLAPGSGVVTKYLLKSGL-------------------QEYLNEQGFNIVG 615

Query: 122 EYGCTTYIGNSGDLDEEVASAISDDE 148
            YGCTT IGNSG+++E V +AI++++
Sbjct: 616 -YGCTTCIGNSGEINESVGAAITEND 621

[Close]

BLAST of CSPI03G00500 vs. TAIR10
Match: AT4G26970.1 (AT4G26970.1 aconitase 2)

HSP 1 Score: 153.7 bits (387), Expect = 9.0e-38
Identity = 82/142 (57.75%), Postives = 100/142 (70.42%), Query Frame = 1


Query: 2   QSFARPKESQTKVVEFSFHGTTAELRHGDVVIAAIASCTNTSNPSIRLGAALVAKKACEL 61
           + FA PKE Q +VV+FS++G  AE++HG VVIAAI SCTNTSNPS+ +GAALVAKKA +L
Sbjct: 501 KGFAVPKEKQEEVVKFSYNGQPAEIKHGSVVIAAITSCTNTSNPSVMIGAALVAKKASDL 560

Query: 62  SLEVKPWINTGLAPGARVVTKYLEKSSIILLTGSFSVSLLVVADVVWRKYLDKLGFNTVG 121
            L+VKPW+ T LAPG+RVV KYL++S +                   R+ L K GF  VG
Sbjct: 561 GLKVKPWVKTSLAPGSRVVEKYLDRSGL-------------------RESLTKQGFEIVG 620

Query: 122 EYGCTTYIGNSGDLDEEVASAI 144
            YGCTT IGNSG+LD EVASAI
Sbjct: 621 -YGCTTCIGNSGNLDPEVASAI 622

[Close]

BLAST of CSPI03G00500 vs. NCBI nr
Match: gi|700200501|gb|KGN55634.1| (hypothetical protein Csa_3G002540 [Cucumis sativus])

HSP 1 Score: 274.2 bits (700), Expect = 1.3e-70
Identity = 143/156 (91.67%), Postives = 145/156 (92.95%), Query Frame = 1


Query: 2   QSFARPKESQTKVVEFSFHGTTAELRHGDVVIAAIASCTNTSNPSIRLGAALVAKKACEL 61
           +SFARPKESQTKVVEFSFHGTTAELRHGDVVIAAIASCTNTSNPSIRLGAALVAKK CEL
Sbjct: 29  KSFARPKESQTKVVEFSFHGTTAELRHGDVVIAAIASCTNTSNPSIRLGAALVAKKVCEL 88

Query: 62  SLEV----------KPWINTGLAPGARVVTKYLEKSSIILLTGSFSVSLLVVADVVWRKY 121
           SLEV          KPWINTGLAPG+RVVTKYLEKSSIILLTGSFSVSLLVVADVVWRKY
Sbjct: 89  SLEVCKFYLLTLPVKPWINTGLAPGSRVVTKYLEKSSIILLTGSFSVSLLVVADVVWRKY 148

Query: 122 LDKLGFNTVGEYGCTTYIGNSGDLDEEVASAISDDE 148
           LDKLGFNTVGEYGCTTYIGNSGDLDEEVASAISDDE
Sbjct: 149 LDKLGFNTVGEYGCTTYIGNSGDLDEEVASAISDDE 184

[Close]

BLAST of CSPI03G00500 vs. NCBI nr
Match: gi|470102662|ref|XP_004287770.1| (PREDICTED: aconitate hydratase 1 [Fragaria vesca subsp. vesca])

HSP 1 Score: 186.8 bits (473), Expect = 2.7e-44
Identity = 98/146 (67.12%), Postives = 113/146 (77.40%), Query Frame = 1


Query: 2   QSFARPKESQTKVVEFSFHGTTAELRHGDVVIAAIASCTNTSNPSIRLGAALVAKKACEL 61
           + FA PKESQTKVVEFSFHGT A+LRHGDVVIAAI SCTNTSNPS+ LGAALVAKKAC+L
Sbjct: 404 KGFAVPKESQTKVVEFSFHGTPAQLRHGDVVIAAITSCTNTSNPSVMLGAALVAKKACDL 463

Query: 62  SLEVKPWINTGLAPGARVVTKYLEKSSIILLTGSFSVSLLVVADVVWRKYLDKLGFNTVG 121
            LEVKPWI T LAPG+ VVTKYLEKS +                   +KYL++LGFN VG
Sbjct: 464 GLEVKPWIKTSLAPGSGVVTKYLEKSGL-------------------QKYLNQLGFNIVG 523

Query: 122 EYGCTTYIGNSGDLDEEVASAISDDE 148
            YGCTT IGNSGD+D+ VASAI++++
Sbjct: 524 -YGCTTCIGNSGDIDDAVASAITEND 529

[Close]

BLAST of CSPI03G00500 vs. NCBI nr
Match: gi|147855123|emb|CAN83844.1| (hypothetical protein VITISV_003004 [Vitis vinifera])

HSP 1 Score: 184.1 bits (466), Expect = 1.8e-43
Identity = 97/146 (66.44%), Postives = 113/146 (77.40%), Query Frame = 1


Query: 2   QSFARPKESQTKVVEFSFHGTTAELRHGDVVIAAIASCTNTSNPSIRLGAALVAKKACEL 61
           + FA PKESQ+KVVEFS+HGT A+LRHGDVVIAAI SCTNTSNPS+ LGAALVAKKACEL
Sbjct: 357 KGFAIPKESQSKVVEFSYHGTPAQLRHGDVVIAAITSCTNTSNPSVMLGAALVAKKACEL 416

Query: 62  SLEVKPWINTGLAPGARVVTKYLEKSSIILLTGSFSVSLLVVADVVWRKYLDKLGFNTVG 121
            LEVKPWI T LAPG+ VVTKYLEKS +                   +KYL++LGF+ VG
Sbjct: 417 GLEVKPWIKTSLAPGSGVVTKYLEKSGL-------------------QKYLNQLGFHIVG 476

Query: 122 EYGCTTYIGNSGDLDEEVASAISDDE 148
            YGCTT IGNSGD++E VASAIS+++
Sbjct: 477 -YGCTTCIGNSGDINESVASAISEND 482

[Close]

BLAST of CSPI03G00500 vs. NCBI nr
Match: gi|296084058|emb|CBI24446.3| (unnamed protein product [Vitis vinifera])

HSP 1 Score: 184.1 bits (466), Expect = 1.8e-43
Identity = 97/146 (66.44%), Postives = 113/146 (77.40%), Query Frame = 1


Query: 2   QSFARPKESQTKVVEFSFHGTTAELRHGDVVIAAIASCTNTSNPSIRLGAALVAKKACEL 61
           + FA PKESQ+KVVEFS+HGT A+LRHGDVVIAAI SCTNTSNPS+ LGAALVAKKACEL
Sbjct: 422 KGFAIPKESQSKVVEFSYHGTPAQLRHGDVVIAAITSCTNTSNPSVMLGAALVAKKACEL 481

Query: 62  SLEVKPWINTGLAPGARVVTKYLEKSSIILLTGSFSVSLLVVADVVWRKYLDKLGFNTVG 121
            LEVKPWI T LAPG+ VVTKYLEKS +                   +KYL++LGF+ VG
Sbjct: 482 GLEVKPWIKTSLAPGSGVVTKYLEKSGL-------------------QKYLNQLGFHIVG 541

Query: 122 EYGCTTYIGNSGDLDEEVASAISDDE 148
            YGCTT IGNSGD++E VASAIS+++
Sbjct: 542 -YGCTTCIGNSGDINESVASAISEND 547

[Close]

BLAST of CSPI03G00500 vs. NCBI nr
Match: gi|225468576|ref|XP_002263337.1| (PREDICTED: aconitate hydratase 1 [Vitis vinifera])

HSP 1 Score: 184.1 bits (466), Expect = 1.8e-43
Identity = 97/146 (66.44%), Postives = 113/146 (77.40%), Query Frame = 1


Query: 2   QSFARPKESQTKVVEFSFHGTTAELRHGDVVIAAIASCTNTSNPSIRLGAALVAKKACEL 61
           + FA PKESQ+KVVEFS+HGT A+LRHGDVVIAAI SCTNTSNPS+ LGAALVAKKACEL
Sbjct: 404 KGFAIPKESQSKVVEFSYHGTPAQLRHGDVVIAAITSCTNTSNPSVMLGAALVAKKACEL 463

Query: 62  SLEVKPWINTGLAPGARVVTKYLEKSSIILLTGSFSVSLLVVADVVWRKYLDKLGFNTVG 121
            LEVKPWI T LAPG+ VVTKYLEKS +                   +KYL++LGF+ VG
Sbjct: 464 GLEVKPWIKTSLAPGSGVVTKYLEKSGL-------------------QKYLNQLGFHIVG 523

Query: 122 EYGCTTYIGNSGDLDEEVASAISDDE 148
            YGCTT IGNSGD++E VASAIS+++
Sbjct: 524 -YGCTTCIGNSGDINESVASAISEND 529

The following BLAST results are available for this feature:

BLAST of CSPI03G00500 vs. Swiss-Prot


Match Name	E-value	Identity	Description
ACO1_ARATH	3.9e-43	63.70	Aconitate hydratase 1 OS=Arabidopsis thaliana GN=ACO1 PE=1 SV=2	[more]
ACOC_ORYSJ	4.8e-41	60.96	Putative aconitate hydratase, cytoplasmic OS=Oryza sativa subsp. japonica GN=Os0...	[more]
ACOC_SOLTU	1.1e-40	62.33	Aconitate hydratase, cytoplasmic (Fragment) OS=Solanum tuberosum PE=2 SV=1	[more]
ACOC_CUCMA	2.4e-40	60.27	Aconitate hydratase, cytoplasmic OS=Cucurbita maxima PE=2 SV=1	[more]
ACOC_CUCMC	1.7e-38	60.96	Aconitate hydratase (Fragment) OS=Cucumis melo var. conomon GN=ACO PE=2 SV=1	[more]

BLAST of CSPI03G00500 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L3M2_CUCSA	9.0e-71	91.67	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G002540 PE=4 SV=1	[more]
M0RSV3_MUSAM	1.2e-43	64.63	Uncharacterized protein OS=Musa acuminata subsp. malaccensis PE=4 SV=1	[more]
A5C294_VITVI	1.2e-43	66.44	Aconitate hydratase OS=Vitis vinifera GN=VITISV_003004 PE=3 SV=1	[more]
D7T1R6_VITVI	1.2e-43	66.44	Aconitate hydratase OS=Vitis vinifera GN=VIT_00s0264g00030 PE=3 SV=1	[more]
B9MXB0_POPTR	1.6e-43	66.44	Aconitate hydratase OS=Populus trichocarpa GN=POPTR_0005s10990g PE=3 SV=1	[more]

BLAST of CSPI03G00500 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G35830.1	2.2e-44	63.70	aconitase 1	[more]
AT2G05710.1	2.1e-39	57.53	aconitase 3	[more]
AT4G26970.1	9.0e-38	57.75	aconitase 2	[more]

BLAST of CSPI03G00500 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700200501\|gb\|KGN55634.1\|	1.3e-70	91.67	hypothetical protein Csa_3G002540 [Cucumis sativus]	[more]
gi\|470102662\|ref\|XP_004287770.1\|	2.7e-44	67.12	PREDICTED: aconitate hydratase 1 [Fragaria vesca subsp. vesca]	[more]
gi\|147855123\|emb\|CAN83844.1\|	1.8e-43	66.44	hypothetical protein VITISV_003004 [Vitis vinifera]	[more]
gi\|296084058\|emb\|CBI24446.3\|	1.8e-43	66.44	unnamed protein product [Vitis vinifera]	[more]
gi\|225468576\|ref\|XP_002263337.1\|	1.8e-43	66.44	PREDICTED: aconitate hydratase 1 [Vitis vinifera]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR001030	Acoase/IPM_deHydtase_lsu_aba
IPR015931	Acnase/IPM_dHydase_lsu_aba_1/3
IPR015937	Aconitase/isopropylmalate dehydratase

Vocabulary: **Biological Process**
Term	Definition
GO:0008152	metabolic process

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006996	organelle organization
biological_process	GO:0006972	hyperosmotic response
biological_process	GO:0006833	water transport
biological_process	GO:0007033	vacuole organization
biological_process	GO:0009266	response to temperature stimulus
biological_process	GO:0000302	response to reactive oxygen species
biological_process	GO:0010039	response to iron ion
biological_process	GO:0046686	response to cadmium ion
biological_process	GO:0019643	reductive tricarboxylic acid cycle
biological_process	GO:0009628	response to abiotic stimulus
biological_process	GO:0006102	isocitrate metabolic process
biological_process	GO:0055072	iron ion homeostasis
biological_process	GO:0015979	photosynthesis
biological_process	GO:0007030	Golgi organization
biological_process	GO:0072350	tricarboxylic acid metabolic process
biological_process	GO:0010038	response to metal ion
biological_process	GO:0046487	glyoxylate metabolic process
biological_process	GO:0044724	single-organism carbohydrate catabolic process
biological_process	GO:0006950	response to stress
biological_process	GO:0008152	metabolic process
biological_process	GO:0006007	glucose catabolic process
biological_process	GO:0006096	glycolytic process
cellular_component	GO:0044444	cytoplasmic part
cellular_component	GO:0048046	apoplast
cellular_component	GO:0005829	cytosol
cellular_component	GO:0005886	plasma membrane
cellular_component	GO:0009506	plasmodesma
cellular_component	GO:0005773	vacuole
molecular_function	GO:0051536	iron-sulfur cluster binding
molecular_function	GO:0016829	lyase activity
molecular_function	GO:0051539	4 iron, 4 sulfur cluster binding
molecular_function	GO:0003994	aconitate hydratase activity
molecular_function	GO:0005507	copper ion binding
molecular_function	GO:0048027	mRNA 5'-UTR binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI03G00500.1	CSPI03G00500.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001030	Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha domain	PFAM	PF00330	Aconitase	coord: 11..143 score: 1.3
IPR001030	Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha domain	unknown	SSF53732	Aconitase iron-sulfur domain	coord: 5..145 score: 1.28
IPR015931	Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha, subdomain 1/3	GENE3D	G3DSA:3.30.499.10		coord: 9..143 score: 9.5
IPR015937	Aconitase/isopropylmalate dehydratase	PANTHER	PTHR11670	ACONITASE	coord: 20..85 score: 1.8E-55coord: 105..147 score: 1.8
None	No IPR available	PANTHER	PTHR11670:SF40	ACONITATE HYDRATASE 1	coord: 20..85 score: 1.8E-55coord: 105..147 score: 1.8

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CSPI03G00500	Csa3G002540	Cucumber (Chinese Long) v2	cpicuB120
CSPI03G00500	CsaV3_3G000520	Cucumber (Chinese Long) v3	cpicucB144
CSPI03G00500	Cp4.1LG10g10520	Cucurbita pepo (Zucchini)	cpecpiB064

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CSPI03G00500	Cucumber (Gy14) v1	cgycpiB025
CSPI03G00500	Cucumber (Gy14) v1	cgycpiB484
CSPI03G00500	Cucurbita maxima (Rimu)	cmacpiB332
CSPI03G00500	Cucurbita maxima (Rimu)	cmacpiB666
CSPI03G00500	Cucurbita maxima (Rimu)	cmacpiB840
CSPI03G00500	Cucurbita maxima (Rimu)	cmacpiB871
CSPI03G00500	Cucurbita moschata (Rifu)	cmocpiB323
CSPI03G00500	Cucurbita moschata (Rifu)	cmocpiB658
CSPI03G00500	Cucurbita moschata (Rifu)	cmocpiB825
CSPI03G00500	Cucurbita moschata (Rifu)	cmocpiB852
CSPI03G00500	Cucumber (Chinese Long) v2	cpicuB156
CSPI03G00500	Melon (DHL92) v3.5.1	cpimeB240
CSPI03G00500	Melon (DHL92) v3.5.1	cpimeB258
CSPI03G00500	Watermelon (Charleston Gray)	cpiwcgB190
CSPI03G00500	Watermelon (Charleston Gray)	cpiwcgB212
CSPI03G00500	Watermelon (97103) v1	cpiwmB200
CSPI03G00500	Watermelon (97103) v1	cpiwmB262
CSPI03G00500	Cucurbita pepo (Zucchini)	cpecpiB215
CSPI03G00500	Cucurbita pepo (Zucchini)	cpecpiB485
CSPI03G00500	Cucurbita pepo (Zucchini)	cpecpiB557
CSPI03G00500	Cucurbita pepo (Zucchini)	cpecpiB853
CSPI03G00500	Bottle gourd (USVL1VR-Ls)	cpilsiB164
CSPI03G00500	Bottle gourd (USVL1VR-Ls)	cpilsiB227
CSPI03G00500	Melon (DHL92) v3.6.1	cpimedB225
CSPI03G00500	Melon (DHL92) v3.6.1	cpimedB254
CSPI03G00500	Cucumber (Gy14) v2	cgybcpiB110
CSPI03G00500	Cucumber (Gy14) v2	cgybcpiB272
CSPI03G00500	Silver-seed gourd	carcpiB0179
CSPI03G00500	Silver-seed gourd	carcpiB0441
CSPI03G00500	Silver-seed gourd	carcpiB0467
CSPI03G00500	Silver-seed gourd	carcpiB0671
CSPI03G00500	Cucumber (Chinese Long) v3	cpicucB184
CSPI03G00500	Watermelon (97103) v2	cpiwmbB182
CSPI03G00500	Watermelon (97103) v2	cpiwmbB184
CSPI03G00500	Wax gourd	cpiwgoB226
CSPI03G00500	Wax gourd	cpiwgoB329
CSPI03G00500	Wild cucumber (PI 183967)	cpicpiB133