CSPI03G00490 (gene) Wild cucumber (PI 183967)

NameCSPI03G00490
Typegene
OrganismCucumis sativus (Wild cucumber (PI 183967))
DescriptionAconitate hydratase
LocationChr3 : 368854 .. 369865 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAGTCGTTGGAAACCATGAGGTGATGATCTTTGGTACTTTTGCCAATATAAGACCTGTCAATAAACTATTAGATGGAGAGGTTGGACCTAAAACTATTCACTTTCCCACTGGAGAGAAACTTCCCATCTTTGATGTTGCCATGGTACGGATATCTTTCTTTCGTCTTCAGCAAGAAATTTGTTATTTATTTCCTTTTATCCTCTTTCAGTCAATTAAGTTGATGGGTATATTTACTCTTGACTCAATACTAACTTGACAGACATACAAGAGTGAGGGTCATGATACCATTGTTCTTGCGGGATCGATTATGGAAGTGGGAGTTCCCAGGATTGGGCTGCTAAAGGACCAATGCTTCTGGTAAGTTTCCAACAGCGTCTGTATTCAATTGAGAATTTGTATTTGCCTTTTCTAGTGATAGCTTAAGTATTGTATTCGGTGACAGGGAGTAAAAGCAGTGATTGCTAAAAGCTTTGAGCATACTCATCGCAGTAATTTGGTGGGAATGGGAATAATTACTCTCTGCTTTAAGCAAGGAGAAGATACTGAATCTCTTGGTCTGTCGGGTCGCAAATGCTGTAACATTGATCTTCCAAACAGCATCAAGGATCTCAAACCATTTCCAGATATAACTGGTACGACAGAAGAAGCTGAAACTAGGAAGTCCTTTCAATGCACCCTAAGAGTTAACACAGAGGTATATCAAATCAGCTAGCTTATATCGTCAAAATTTTGGCCTTGTTAAGATTAGGAAAGTTCAGGCATTGGTTAAAAATGGGTGCAGGTTGAACTCACTTATTTTGATCATGGAGGAATTCTACACTACGTGATAAGAAGTTTGATCAACACACAAGACTGAGTCATTAACAAGGCCAGCTTGGATGGTCGAGGCATTTCTCTTCTTCATGGACATAACAAAAACCAGATTCTCCACGTTTCTACACAAGACTTACGAGTATCCAGATCCAGGATATGAAGCAAATTTCCATTTTGCTTTCCAGCTTATGTGA

mRNA sequence

ATGGCAGTCGTTGGAAACCATGAGGTGATGATCTTTGGTACTTTTGCCAATATAAGACCTGTCAATAAACTATTAGATGGAGAGGTTGGACCTAAAACTATTCACTTTCCCACTGGAGAGAAACTTCCCATCTTTGATGTTGCCATGACATACAAGAGTGAGGGTCATGATACCATTGTTCTTGCGGGATCGATTATGGAAGTGGGAGTTCCCAGGATTGGGCTGCTAAAGGACCAATGCTTCTGTAATTTGGTGGGAATGGGAATAATTACTCTCTGCTTTAAGCAAGGAGAAGATACTGAATCTCTTGGTCTGTCGGGTCGCAAATGCTGTAACATTGATCTTCCAAACAGCATCAAGGATCTCAAACCATTTCCAGATATAACTGGTACGACAGAAGAAGCTGAAACTAGGAAGTCCTTTCAATGCACCCTAAGAGTTAACACAGAGACTGAGTCATTAACAAGGCCAGCTTGGATGGTCGAGGCATTTCTCTTCTTCATGGACATAACAAAAACCAGATTCTCCACGTTTCTACACAAGACTTACGAGTATCCAGATCCAGGATATGAAGCAAATTTCCATTTTGCTTTCCAGCTTATGTGA

Coding sequence (CDS)

ATGGCAGTCGTTGGAAACCATGAGGTGATGATCTTTGGTACTTTTGCCAATATAAGACCTGTCAATAAACTATTAGATGGAGAGGTTGGACCTAAAACTATTCACTTTCCCACTGGAGAGAAACTTCCCATCTTTGATGTTGCCATGACATACAAGAGTGAGGGTCATGATACCATTGTTCTTGCGGGATCGATTATGGAAGTGGGAGTTCCCAGGATTGGGCTGCTAAAGGACCAATGCTTCTGTAATTTGGTGGGAATGGGAATAATTACTCTCTGCTTTAAGCAAGGAGAAGATACTGAATCTCTTGGTCTGTCGGGTCGCAAATGCTGTAACATTGATCTTCCAAACAGCATCAAGGATCTCAAACCATTTCCAGATATAACTGGTACGACAGAAGAAGCTGAAACTAGGAAGTCCTTTCAATGCACCCTAAGAGTTAACACAGAGACTGAGTCATTAACAAGGCCAGCTTGGATGGTCGAGGCATTTCTCTTCTTCATGGACATAACAAAAACCAGATTCTCCACGTTTCTACACAAGACTTACGAGTATCCAGATCCAGGATATGAAGCAAATTTCCATTTTGCTTTCCAGCTTATGTGA
BLAST of CSPI03G00490 vs. Swiss-Prot
Match: ACOC_ORYSJ (Putative aconitate hydratase, cytoplasmic OS=Oryza sativa subsp. japonica GN=Os08g0191100 PE=3 SV=1)

HSP 1 Score: 164.5 bits (415), Expect = 1.2e-39
Identity = 89/164 (54.27%), Postives = 104/164 (63.41%), Query Frame = 1

Query: 5   GNHEVMIFGTFANIRPVNKLLDGEVGPKTIHFPTGEKLPIFDVAMTYKSEGHDTIVLAGS 64
           GN EVM  GTFANIR VNK L+GEVGPKT+H PTGEKL +FD A+ YKSEGHDTIVLAG+
Sbjct: 718 GNDEVMARGTFANIRIVNKFLNGEVGPKTVHVPTGEKLYVFDAALKYKSEGHDTIVLAGA 777

Query: 65  IMEVGVPRIGLLKDQCF----------------CNLVGMGIITLCFKQGEDTESLGLSGR 124
               G  R    K                     NLVGMGII LCFK GED +SLGL+G 
Sbjct: 778 EYGSGSSRDWAAKGPMLLGVKAVIAKSFERIHRSNLVGMGIIPLCFKAGEDADSLGLTGH 837

Query: 125 KCCNIDLPNSIKDLKPFPDITGTTEEAETRKSFQCTLRVNTETE 153
           +   IDLP ++ +++P  DIT TT+     KSF CTLR +TE E
Sbjct: 838 ERYTIDLPTNVSEIRPGQDITVTTDNG---KSFTCTLRFDTEVE 878

BLAST of CSPI03G00490 vs. Swiss-Prot
Match: ACO1_ARATH (Aconitate hydratase 1 OS=Arabidopsis thaliana GN=ACO1 PE=1 SV=2)

HSP 1 Score: 158.7 bits (400), Expect = 6.8e-38
Identity = 84/164 (51.22%), Postives = 102/164 (62.20%), Query Frame = 1

Query: 5   GNHEVMIFGTFANIRPVNKLLDGEVGPKTIHFPTGEKLPIFDVAMTYKSEGHDTIVLAGS 64
           GN E+M  GTFANIR VNK L GEVGPKT+H PTGEKL +FD AM Y++EG DTI+LAG+
Sbjct: 718 GNDEIMARGTFANIRIVNKHLKGEVGPKTVHIPTGEKLSVFDAAMKYRNEGRDTIILAGA 777

Query: 65  IMEVGVPRIGLLKDQCF----------------CNLVGMGIITLCFKQGEDTESLGLSGR 124
               G  R    K                     NLVGMGII LCFK GED E+LGL+G+
Sbjct: 778 EYGSGSSRDWAAKGPMLLGVKAVISKSFERIHRSNLVGMGIIPLCFKAGEDAETLGLTGQ 837

Query: 125 KCCNIDLPNSIKDLKPFPDITGTTEEAETRKSFQCTLRVNTETE 153
           +   I+LPN++ ++KP  D+T  T      KSF CTLR +TE E
Sbjct: 838 ELYTIELPNNVSEIKPGQDVTVVTNNG---KSFTCTLRFDTEVE 878

BLAST of CSPI03G00490 vs. Swiss-Prot
Match: ACOC_CUCMA (Aconitate hydratase, cytoplasmic OS=Cucurbita maxima PE=2 SV=1)

HSP 1 Score: 158.3 bits (399), Expect = 8.8e-38
Identity = 87/164 (53.05%), Postives = 101/164 (61.59%), Query Frame = 1

Query: 5   GNHEVMIFGTFANIRPVNKLLDGEVGPKTIHFPTGEKLPIFDVAMTYKSEGHDTIVLAGS 64
           GN EVM  GTFANIR VNKLLDGEVGPKT+H PTGEKL +F+ A  YKS G DTIVLAG+
Sbjct: 718 GNDEVMARGTFANIRLVNKLLDGEVGPKTVHVPTGEKLSVFEAAEKYKSAGQDTIVLAGA 777

Query: 65  IMEVGVPRIGLLKDQCF----------------CNLVGMGIITLCFKQGEDTESLGLSGR 124
               G  R    K                     NLVGMGII LCFK GED +SLGL+G 
Sbjct: 778 EYGSGSSRDWAAKGPMLLGVKAVIAKSFERIHRSNLVGMGIIPLCFKSGEDADSLGLTGH 837

Query: 125 KCCNIDLPNSIKDLKPFPDITGTTEEAETRKSFQCTLRVNTETE 153
           +   IDLP+ I  ++P  D+T TT+     KSF CT+R +TE E
Sbjct: 838 ERYTIDLPDDISKIRPGQDVTVTTDSG---KSFTCTVRFDTEVE 878

BLAST of CSPI03G00490 vs. Swiss-Prot
Match: ACO2M_ARATH (Aconitate hydratase 2, mitochondrial OS=Arabidopsis thaliana GN=ACO2 PE=1 SV=2)

HSP 1 Score: 155.2 bits (391), Expect = 7.5e-37
Identity = 82/164 (50.00%), Postives = 101/164 (61.59%), Query Frame = 1

Query: 5   GNHEVMIFGTFANIRPVNKLLDGEVGPKTIHFPTGEKLPIFDVAMTYKSEGHDTIVLAGS 64
           GN E+M  GTFANIR VNKL++GEVGPKT+H P+GEKL +FD AM YKS G DTI+LAG+
Sbjct: 810 GNDEIMARGTFANIRIVNKLMNGEVGPKTVHIPSGEKLSVFDAAMRYKSSGEDTIILAGA 869

Query: 65  IMEVGVPRIGLLKDQCF----------------CNLVGMGIITLCFKQGEDTESLGLSGR 124
               G  R    K                     NLVGMGII LCFK GED ++LGL+G 
Sbjct: 870 EYGSGSSRDWAAKGPMLQGVKAVIAKSFERIHRSNLVGMGIIPLCFKSGEDADTLGLTGH 929

Query: 125 KCCNIDLPNSIKDLKPFPDITGTTEEAETRKSFQCTLRVNTETE 153
           +   I LP  I +++P  D+T TT+     KSF CT+R +TE E
Sbjct: 930 ERYTIHLPTDISEIRPGQDVTVTTDNG---KSFTCTVRFDTEVE 970

BLAST of CSPI03G00490 vs. Swiss-Prot
Match: ACOC_SOLTU (Aconitate hydratase, cytoplasmic (Fragment) OS=Solanum tuberosum PE=2 SV=1)

HSP 1 Score: 151.8 bits (382), Expect = 8.3e-36
Identity = 81/164 (49.39%), Postives = 102/164 (62.20%), Query Frame = 1

Query: 5   GNHEVMIFGTFANIRPVNKLLDGEVGPKTIHFPTGEKLPIFDVAMTYKSEGHDTIVLAGS 64
           GN E+M  GTFANIR VNKLL+GEVGPKT+H P+GEKL +FD AM YKS G  TI+LAG+
Sbjct: 436 GNDEIMARGTFANIRLVNKLLNGEVGPKTVHVPSGEKLSVFDAAMKYKSAGQSTIILAGA 495

Query: 65  IMEVGVPRIGLLKDQCF----------------CNLVGMGIITLCFKQGEDTESLGLSGR 124
               G  R    K                     NLVGMGI+ LCFK GED ++LGL+G+
Sbjct: 496 EYGSGSSRDWAAKGPMLLGVKAVIAKSFERIHRSNLVGMGIVPLCFKAGEDADTLGLTGQ 555

Query: 125 KCCNIDLPNSIKDLKPFPDITGTTEEAETRKSFQCTLRVNTETE 153
           +   IDLP +I +++P  D+T  T   +T KSF C +R +TE E
Sbjct: 556 ERYTIDLPENISEIRPGQDVTVQT---DTGKSFTCIVRFDTEVE 596

BLAST of CSPI03G00490 vs. TrEMBL
Match: A0A0A0L1K8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G002530 PE=4 SV=1)

HSP 1 Score: 203.4 bits (516), Expect = 2.7e-49
Identity = 106/154 (68.83%), Postives = 112/154 (72.73%), Query Frame = 1

Query: 2   AVVGNHEVMIFGTFANIRPVNKLLDGEVGPKTIHFPTGEKLPIFDVAMTYKSEGHDTIVL 61
           +VVGNHEVMIFGTFANIRPVNKLLDGEVGPKTI FP    LP     M       D    
Sbjct: 57  SVVGNHEVMIFGTFANIRPVNKLLDGEVGPKTITFPLERNLPSL---MLPCGSSQDWAAK 116

Query: 62  AGSIMEVGVPRIGLLKDQCFCNLVGMGIITLCFKQGEDTESLGLSGRKCCNIDLPNSIKD 121
              ++ V        +     NLVGMGIITLCFKQGEDTESLGLSG KCCNIDLPNSIKD
Sbjct: 117 GPMLLGVKAVIAKSFEHTHRSNLVGMGIITLCFKQGEDTESLGLSGHKCCNIDLPNSIKD 176

Query: 122 LKPFPDITGTTEEAETRKSFQCTLRVNTETESLT 156
           LKPFPDITGTTEEAETRKSFQCTLRVNTE   ++
Sbjct: 177 LKPFPDITGTTEEAETRKSFQCTLRVNTEVYQIS 207

BLAST of CSPI03G00490 vs. TrEMBL
Match: A0A059AHS0_EUCGR (Aconitate hydratase OS=Eucalyptus grandis GN=EUGRSUZ_J02488 PE=3 SV=1)

HSP 1 Score: 169.1 bits (427), Expect = 5.6e-39
Identity = 89/164 (54.27%), Postives = 106/164 (64.63%), Query Frame = 1

Query: 5   GNHEVMIFGTFANIRPVNKLLDGEVGPKTIHFPTGEKLPIFDVAMTYKSEGHDTIVLAGS 64
           GN+EVM+ GTFAN+R VNKLL+GEVGP+TIH P+GEK+ +FD AM YK+EG DTIVLAGS
Sbjct: 746 GNYEVMVRGTFANVRIVNKLLEGEVGPRTIHIPSGEKMHVFDAAMRYKNEGQDTIVLAGS 805

Query: 65  IMEVGVPRIGLLKDQCF----------------CNLVGMGIITLCFKQGEDTESLGLSGR 124
              VG  R G  K                     NLVGMGII LCFK GED E+LGL+G 
Sbjct: 806 EYGVGSSRDGAAKGPMLVGVQAVIAKSFEQIHRSNLVGMGIIPLCFKPGEDAETLGLNGH 865

Query: 125 KCCNIDLPNSIKDLKPFPDITGTTEEAETRKSFQCTLRVNTETE 153
           +   IDLPN + +LKP  D+   T+     KSF CTLR +TE E
Sbjct: 866 ERYTIDLPNKVSELKPRQDVKVVTDNG---KSFACTLRFDTEVE 906

BLAST of CSPI03G00490 vs. TrEMBL
Match: A0A0E0LS31_ORYPU (Aconitate hydratase OS=Oryza punctata PE=3 SV=1)

HSP 1 Score: 166.0 bits (419), Expect = 4.7e-38
Identity = 91/164 (55.49%), Postives = 104/164 (63.41%), Query Frame = 1

Query: 5   GNHEVMIFGTFANIRPVNKLLDGEVGPKTIHFPTGEKLPIFDVAMTYKSEGHDTIVLAGS 64
           GN EVM  GTFANIR VNK L+GEVGPKT+H PTGEKL +FD AM YKSEGHDTIVLAG+
Sbjct: 811 GNDEVMARGTFANIRIVNKFLNGEVGPKTVHVPTGEKLYVFDAAMKYKSEGHDTIVLAGA 870

Query: 65  IMEVGVPRIGLLKDQCF----------------CNLVGMGIITLCFKQGEDTESLGLSGR 124
               G  R    K                     NLVGMGII LCFK GED +SLGL+G 
Sbjct: 871 EYGSGSSRDWAAKGPMLLGVKAVIAKSFERIHRSNLVGMGIIPLCFKAGEDADSLGLTGH 930

Query: 125 KCCNIDLPNSIKDLKPFPDITGTTEEAETRKSFQCTLRVNTETE 153
           +   IDLP +I +++P  DIT TT+     KSF CTLR +TE E
Sbjct: 931 ERYTIDLPTNISEIRPGQDITVTTDNG---KSFTCTLRFDTEVE 971

BLAST of CSPI03G00490 vs. TrEMBL
Match: A0A0E0LS32_ORYPU (Uncharacterized protein OS=Oryza punctata PE=4 SV=1)

HSP 1 Score: 166.0 bits (419), Expect = 4.7e-38
Identity = 91/164 (55.49%), Postives = 104/164 (63.41%), Query Frame = 1

Query: 5   GNHEVMIFGTFANIRPVNKLLDGEVGPKTIHFPTGEKLPIFDVAMTYKSEGHDTIVLAGS 64
           GN EVM  GTFANIR VNK L+GEVGPKT+H PTGEKL +FD AM YKSEGHDTIVLAG+
Sbjct: 833 GNDEVMARGTFANIRIVNKFLNGEVGPKTVHVPTGEKLYVFDAAMKYKSEGHDTIVLAGA 892

Query: 65  IMEVGVPRIGLLKDQCF----------------CNLVGMGIITLCFKQGEDTESLGLSGR 124
               G  R    K                     NLVGMGII LCFK GED +SLGL+G 
Sbjct: 893 EYGSGSSRDWAAKGPMLLGVKAVIAKSFERIHRSNLVGMGIIPLCFKAGEDADSLGLTGH 952

Query: 125 KCCNIDLPNSIKDLKPFPDITGTTEEAETRKSFQCTLRVNTETE 153
           +   IDLP +I +++P  DIT TT+     KSF CTLR +TE E
Sbjct: 953 ERYTIDLPTNISEIRPGQDITVTTDNG---KSFTCTLRFDTEVE 993

BLAST of CSPI03G00490 vs. TrEMBL
Match: J3MR12_ORYBR (Aconitate hydratase OS=Oryza brachyantha PE=3 SV=1)

HSP 1 Score: 166.0 bits (419), Expect = 4.7e-38
Identity = 91/164 (55.49%), Postives = 104/164 (63.41%), Query Frame = 1

Query: 5   GNHEVMIFGTFANIRPVNKLLDGEVGPKTIHFPTGEKLPIFDVAMTYKSEGHDTIVLAGS 64
           GN EVM  GTFANIR VNK L+GEVGPKTIH PTGEKL +FD AM YKSEGHDTIVLAG+
Sbjct: 811 GNDEVMARGTFANIRIVNKFLNGEVGPKTIHVPTGEKLYVFDAAMKYKSEGHDTIVLAGA 870

Query: 65  IMEVGVPRIGLLKDQCF----------------CNLVGMGIITLCFKQGEDTESLGLSGR 124
               G  R    K                     NLVGMGII LCFK GED +SLGL+G 
Sbjct: 871 EYGSGSSRDWAAKGPMLLGVKAVIAKSFERIHRSNLVGMGIIPLCFKAGEDADSLGLTGH 930

Query: 125 KCCNIDLPNSIKDLKPFPDITGTTEEAETRKSFQCTLRVNTETE 153
           +   IDLP +I +++P  D+T TT+     KSF CTLR +TE E
Sbjct: 931 ERYTIDLPTNISEIRPGQDVTVTTDNG---KSFTCTLRFDTEVE 971

BLAST of CSPI03G00490 vs. TAIR10
Match: AT4G35830.1 (AT4G35830.1 aconitase 1)

HSP 1 Score: 158.7 bits (400), Expect = 3.8e-39
Identity = 84/164 (51.22%), Postives = 102/164 (62.20%), Query Frame = 1

Query: 5   GNHEVMIFGTFANIRPVNKLLDGEVGPKTIHFPTGEKLPIFDVAMTYKSEGHDTIVLAGS 64
           GN E+M  GTFANIR VNK L GEVGPKT+H PTGEKL +FD AM Y++EG DTI+LAG+
Sbjct: 718 GNDEIMARGTFANIRIVNKHLKGEVGPKTVHIPTGEKLSVFDAAMKYRNEGRDTIILAGA 777

Query: 65  IMEVGVPRIGLLKDQCF----------------CNLVGMGIITLCFKQGEDTESLGLSGR 124
               G  R    K                     NLVGMGII LCFK GED E+LGL+G+
Sbjct: 778 EYGSGSSRDWAAKGPMLLGVKAVISKSFERIHRSNLVGMGIIPLCFKAGEDAETLGLTGQ 837

Query: 125 KCCNIDLPNSIKDLKPFPDITGTTEEAETRKSFQCTLRVNTETE 153
           +   I+LPN++ ++KP  D+T  T      KSF CTLR +TE E
Sbjct: 838 ELYTIELPNNVSEIKPGQDVTVVTNNG---KSFTCTLRFDTEVE 878

BLAST of CSPI03G00490 vs. TAIR10
Match: AT2G05710.1 (AT2G05710.1 aconitase 3)

HSP 1 Score: 155.2 bits (391), Expect = 4.2e-38
Identity = 82/164 (50.00%), Postives = 101/164 (61.59%), Query Frame = 1

Query: 5   GNHEVMIFGTFANIRPVNKLLDGEVGPKTIHFPTGEKLPIFDVAMTYKSEGHDTIVLAGS 64
           GN E+M  GTFANIR VNKL++GEVGPKT+H P+GEKL +FD AM YKS G DTI+LAG+
Sbjct: 810 GNDEIMARGTFANIRIVNKLMNGEVGPKTVHIPSGEKLSVFDAAMRYKSSGEDTIILAGA 869

Query: 65  IMEVGVPRIGLLKDQCF----------------CNLVGMGIITLCFKQGEDTESLGLSGR 124
               G  R    K                     NLVGMGII LCFK GED ++LGL+G 
Sbjct: 870 EYGSGSSRDWAAKGPMLQGVKAVIAKSFERIHRSNLVGMGIIPLCFKSGEDADTLGLTGH 929

Query: 125 KCCNIDLPNSIKDLKPFPDITGTTEEAETRKSFQCTLRVNTETE 153
           +   I LP  I +++P  D+T TT+     KSF CT+R +TE E
Sbjct: 930 ERYTIHLPTDISEIRPGQDVTVTTDNG---KSFTCTVRFDTEVE 970

BLAST of CSPI03G00490 vs. TAIR10
Match: AT4G26970.1 (AT4G26970.1 aconitase 2)

HSP 1 Score: 149.1 bits (375), Expect = 3.0e-36
Identity = 81/164 (49.39%), Postives = 96/164 (58.54%), Query Frame = 1

Query: 5   GNHEVMIFGTFANIRPVNKLLDGEVGPKTIHFPTGEKLPIFDVAMTYKSEGHDTIVLAGS 64
           GN EVM  GTFANIR VNKLL GEVGP T+H PTGEKL +FD A  YK+   DTI+LAG+
Sbjct: 815 GNDEVMARGTFANIRIVNKLLKGEVGPNTVHIPTGEKLSVFDAASKYKTAEQDTIILAGA 874

Query: 65  IMEVGVPRIGLLKDQCF----------------CNLVGMGIITLCFKQGEDTESLGLSGR 124
               G  R    K                     NL GMGII LCFK GED E+LGL+G 
Sbjct: 875 EYGSGSSRDWAAKGPLLLGVKAVIAKSFERIHRSNLAGMGIIPLCFKAGEDAETLGLTGH 934

Query: 125 KCCNIDLPNSIKDLKPFPDITGTTEEAETRKSFQCTLRVNTETE 153
           +   + LP  + D++P  D+T TT+     KSF CTLR +TE E
Sbjct: 935 ERYTVHLPTKVSDIRPGQDVTVTTDSG---KSFVCTLRFDTEVE 975

BLAST of CSPI03G00490 vs. TAIR10
Match: AT5G54950.1 (AT5G54950.1 Aconitase family protein)

HSP 1 Score: 62.4 bits (150), Expect = 3.7e-10
Identity = 31/59 (52.54%), Postives = 42/59 (71.19%), Query Frame = 1

Query: 92  LCFKQGEDTESLGLSGRKCCNIDLPNSIKDLKPFPDITGTTEEAETRKSFQCTLRVNTE 151
           + FK GED E+LGL+G +   I LP++I ++KP  DIT TT   +T KSF CTLR++TE
Sbjct: 1   MAFKSGEDAETLGLTGHELYTIHLPSNINEIKPGQDITVTT---DTAKSFVCTLRLDTE 56

BLAST of CSPI03G00490 vs. NCBI nr
Match: gi|700200500|gb|KGN55633.1| (hypothetical protein Csa_3G002530 [Cucumis sativus])

HSP 1 Score: 203.4 bits (516), Expect = 3.8e-49
Identity = 106/154 (68.83%), Postives = 112/154 (72.73%), Query Frame = 1

Query: 2   AVVGNHEVMIFGTFANIRPVNKLLDGEVGPKTIHFPTGEKLPIFDVAMTYKSEGHDTIVL 61
           +VVGNHEVMIFGTFANIRPVNKLLDGEVGPKTI FP    LP     M       D    
Sbjct: 57  SVVGNHEVMIFGTFANIRPVNKLLDGEVGPKTITFPLERNLPSL---MLPCGSSQDWAAK 116

Query: 62  AGSIMEVGVPRIGLLKDQCFCNLVGMGIITLCFKQGEDTESLGLSGRKCCNIDLPNSIKD 121
              ++ V        +     NLVGMGIITLCFKQGEDTESLGLSG KCCNIDLPNSIKD
Sbjct: 117 GPMLLGVKAVIAKSFEHTHRSNLVGMGIITLCFKQGEDTESLGLSGHKCCNIDLPNSIKD 176

Query: 122 LKPFPDITGTTEEAETRKSFQCTLRVNTETESLT 156
           LKPFPDITGTTEEAETRKSFQCTLRVNTE   ++
Sbjct: 177 LKPFPDITGTTEEAETRKSFQCTLRVNTEVYQIS 207

BLAST of CSPI03G00490 vs. NCBI nr
Match: gi|629086864|gb|KCW53221.1| (hypothetical protein EUGRSUZ_J02488 [Eucalyptus grandis])

HSP 1 Score: 169.1 bits (427), Expect = 8.0e-39
Identity = 89/164 (54.27%), Postives = 106/164 (64.63%), Query Frame = 1

Query: 5   GNHEVMIFGTFANIRPVNKLLDGEVGPKTIHFPTGEKLPIFDVAMTYKSEGHDTIVLAGS 64
           GN+EVM+ GTFAN+R VNKLL+GEVGP+TIH P+GEK+ +FD AM YK+EG DTIVLAGS
Sbjct: 746 GNYEVMVRGTFANVRIVNKLLEGEVGPRTIHIPSGEKMHVFDAAMRYKNEGQDTIVLAGS 805

Query: 65  IMEVGVPRIGLLKDQCF----------------CNLVGMGIITLCFKQGEDTESLGLSGR 124
              VG  R G  K                     NLVGMGII LCFK GED E+LGL+G 
Sbjct: 806 EYGVGSSRDGAAKGPMLVGVQAVIAKSFEQIHRSNLVGMGIIPLCFKPGEDAETLGLNGH 865

Query: 125 KCCNIDLPNSIKDLKPFPDITGTTEEAETRKSFQCTLRVNTETE 153
           +   IDLPN + +LKP  D+   T+     KSF CTLR +TE E
Sbjct: 866 ERYTIDLPNKVSELKPRQDVKVVTDNG---KSFACTLRFDTEVE 906

BLAST of CSPI03G00490 vs. NCBI nr
Match: gi|702482502|ref|XP_010033531.1| (PREDICTED: aconitate hydratase 1-like [Eucalyptus grandis])

HSP 1 Score: 169.1 bits (427), Expect = 8.0e-39
Identity = 89/164 (54.27%), Postives = 106/164 (64.63%), Query Frame = 1

Query: 5   GNHEVMIFGTFANIRPVNKLLDGEVGPKTIHFPTGEKLPIFDVAMTYKSEGHDTIVLAGS 64
           GN+EVM+ GTFAN+R VNKLL+GEVGP+TIH P+GEK+ +FD AM YK+EG DTIVLAGS
Sbjct: 719 GNYEVMVRGTFANVRIVNKLLEGEVGPRTIHIPSGEKMHVFDAAMRYKNEGQDTIVLAGS 778

Query: 65  IMEVGVPRIGLLKDQCF----------------CNLVGMGIITLCFKQGEDTESLGLSGR 124
              VG  R G  K                     NLVGMGII LCFK GED E+LGL+G 
Sbjct: 779 EYGVGSSRDGAAKGPMLVGVQAVIAKSFEQIHRSNLVGMGIIPLCFKPGEDAETLGLNGH 838

Query: 125 KCCNIDLPNSIKDLKPFPDITGTTEEAETRKSFQCTLRVNTETE 153
           +   IDLPN + +LKP  D+   T+     KSF CTLR +TE E
Sbjct: 839 ERYTIDLPNKVSELKPRQDVKVVTDNG---KSFACTLRFDTEVE 879

BLAST of CSPI03G00490 vs. NCBI nr
Match: gi|1021557489|ref|XP_016170065.1| (PREDICTED: aconitate hydratase, cytoplasmic-like [Arachis ipaensis])

HSP 1 Score: 168.7 bits (426), Expect = 1.0e-38
Identity = 92/164 (56.10%), Postives = 105/164 (64.02%), Query Frame = 1

Query: 5   GNHEVMIFGTFANIRPVNKLLDGEVGPKTIHFPTGEKLPIFDVAMTYKSEGHDTIVLAGS 64
           GN EVM  GTFANIR VNKLL+GEVGPKT+H PTGEKL +FD AM YK+EGHDTIVLAG+
Sbjct: 811 GNDEVMARGTFANIRIVNKLLNGEVGPKTVHIPTGEKLYVFDAAMRYKNEGHDTIVLAGA 870

Query: 65  IMEVGVPRIGLLKDQCF----------------CNLVGMGIITLCFKQGEDTESLGLSGR 124
               G  R    K                     NLVGMGII LCFK GED E+LGL+G 
Sbjct: 871 EYGSGSSRDWAAKGPMLLGVKAVIAKSFERIHRSNLVGMGIIPLCFKAGEDAETLGLTGH 930

Query: 125 KCCNIDLPNSIKDLKPFPDITGTTEEAETRKSFQCTLRVNTETE 153
           +   IDLPN I D++P  D+T TT    T KSF CT+R +TE E
Sbjct: 931 ERYTIDLPNKISDIRPGQDVTVTT---NTGKSFTCTVRFDTEVE 971

BLAST of CSPI03G00490 vs. NCBI nr
Match: gi|1012222212|ref|XP_015936058.1| (PREDICTED: aconitate hydratase, cytoplasmic-like [Arachis duranensis])

HSP 1 Score: 168.7 bits (426), Expect = 1.0e-38
Identity = 92/164 (56.10%), Postives = 105/164 (64.02%), Query Frame = 1

Query: 5   GNHEVMIFGTFANIRPVNKLLDGEVGPKTIHFPTGEKLPIFDVAMTYKSEGHDTIVLAGS 64
           GN EVM  GTFANIR VNKLL+GEVGPKT+H PTGEKL +FD AM YK+EGHDTIVLAG+
Sbjct: 811 GNDEVMARGTFANIRIVNKLLNGEVGPKTVHIPTGEKLYVFDAAMRYKNEGHDTIVLAGA 870

Query: 65  IMEVGVPRIGLLKDQCF----------------CNLVGMGIITLCFKQGEDTESLGLSGR 124
               G  R    K                     NLVGMGII LCFK GED E+LGL+G 
Sbjct: 871 EYGSGSSRDWAAKGPMLLGVKAVIAKSFERIHRSNLVGMGIIPLCFKAGEDAETLGLTGH 930

Query: 125 KCCNIDLPNSIKDLKPFPDITGTTEEAETRKSFQCTLRVNTETE 153
           +   IDLPN I D++P  D+T TT    T KSF CT+R +TE E
Sbjct: 931 ERYTIDLPNKISDIRPGQDVTVTT---NTGKSFTCTVRFDTEVE 971

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
ACOC_ORYSJ1.2e-3954.27Putative aconitate hydratase, cytoplasmic OS=Oryza sativa subsp. japonica GN=Os0... [more]
ACO1_ARATH6.8e-3851.22Aconitate hydratase 1 OS=Arabidopsis thaliana GN=ACO1 PE=1 SV=2[more]
ACOC_CUCMA8.8e-3853.05Aconitate hydratase, cytoplasmic OS=Cucurbita maxima PE=2 SV=1[more]
ACO2M_ARATH7.5e-3750.00Aconitate hydratase 2, mitochondrial OS=Arabidopsis thaliana GN=ACO2 PE=1 SV=2[more]
ACOC_SOLTU8.3e-3649.39Aconitate hydratase, cytoplasmic (Fragment) OS=Solanum tuberosum PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0L1K8_CUCSA2.7e-4968.83Uncharacterized protein OS=Cucumis sativus GN=Csa_3G002530 PE=4 SV=1[more]
A0A059AHS0_EUCGR5.6e-3954.27Aconitate hydratase OS=Eucalyptus grandis GN=EUGRSUZ_J02488 PE=3 SV=1[more]
A0A0E0LS31_ORYPU4.7e-3855.49Aconitate hydratase OS=Oryza punctata PE=3 SV=1[more]
A0A0E0LS32_ORYPU4.7e-3855.49Uncharacterized protein OS=Oryza punctata PE=4 SV=1[more]
J3MR12_ORYBR4.7e-3855.49Aconitate hydratase OS=Oryza brachyantha PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT4G35830.13.8e-3951.22 aconitase 1[more]
AT2G05710.14.2e-3850.00 aconitase 3[more]
AT4G26970.13.0e-3649.39 aconitase 2[more]
AT5G54950.13.7e-1052.54 Aconitase family protein[more]
Match NameE-valueIdentityDescription
gi|700200500|gb|KGN55633.1|3.8e-4968.83hypothetical protein Csa_3G002530 [Cucumis sativus][more]
gi|629086864|gb|KCW53221.1|8.0e-3954.27hypothetical protein EUGRSUZ_J02488 [Eucalyptus grandis][more]
gi|702482502|ref|XP_010033531.1|8.0e-3954.27PREDICTED: aconitate hydratase 1-like [Eucalyptus grandis][more]
gi|1021557489|ref|XP_016170065.1|1.0e-3856.10PREDICTED: aconitate hydratase, cytoplasmic-like [Arachis ipaensis][more]
gi|1012222212|ref|XP_015936058.1|1.0e-3856.10PREDICTED: aconitate hydratase, cytoplasmic-like [Arachis duranensis][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000573AconitaseA/IPMdHydase_ssu_swvl
IPR015928Aconitase/3IPM_dehydase_swvl
IPR015937Aconitase/isopropylmalate dehydratase
Vocabulary: Biological Process
TermDefinition
GO:0008152metabolic process
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008152 metabolic process
biological_process GO:0010039 response to iron ion
biological_process GO:0072350 tricarboxylic acid metabolic process
biological_process GO:0044724 single-organism carbohydrate catabolic process
biological_process GO:0006950 response to stress
biological_process GO:0010038 response to metal ion
biological_process GO:0009628 response to abiotic stimulus
biological_process GO:0006996 organelle organization
biological_process GO:0006833 water transport
biological_process GO:0007033 vacuole organization
biological_process GO:0009266 response to temperature stimulus
biological_process GO:0000302 response to reactive oxygen species
biological_process GO:0008150 biological_process
biological_process GO:0046686 response to cadmium ion
biological_process GO:0019643 reductive tricarboxylic acid cycle
biological_process GO:0015979 photosynthesis
biological_process GO:0006102 isocitrate metabolic process
biological_process GO:0055072 iron ion homeostasis
biological_process GO:0006007 glucose catabolic process
biological_process GO:0006096 glycolytic process
biological_process GO:0046487 glyoxylate metabolic process
biological_process GO:0007030 Golgi organization
biological_process GO:0006972 hyperosmotic response
cellular_component GO:0044444 cytoplasmic part
cellular_component GO:0005829 cytosol
cellular_component GO:0005773 vacuole
cellular_component GO:0009506 plasmodesma
cellular_component GO:0005886 plasma membrane
cellular_component GO:0005739 mitochondrion
cellular_component GO:0005575 cellular_component
cellular_component GO:0048046 apoplast
molecular_function GO:0051539 4 iron, 4 sulfur cluster binding
molecular_function GO:0005507 copper ion binding
molecular_function GO:0048027 mRNA 5'-UTR binding
molecular_function GO:0003674 molecular_function
molecular_function GO:0051536 iron-sulfur cluster binding
molecular_function GO:0016829 lyase activity
molecular_function GO:0003994 aconitate hydratase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI03G00490.1CSPI03G00490.1mRNA


Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000573Aconitase A/isopropylmalate dehydratase small subunit, swivel domainPFAMPF00694Aconitase_Ccoord: 4..63
score: 2.
IPR015928Aconitase/3-isopropylmalate dehydratase, swivelGENE3DG3DSA:3.20.19.10coord: 5..63
score: 2.0E-10coord: 83..152
score: 1.
IPR015928Aconitase/3-isopropylmalate dehydratase, swivelunknownSSF52016LeuD/IlvD-likecoord: 4..127
score: 3.18
IPR015937Aconitase/isopropylmalate dehydratasePANTHERPTHR11670ACONITASEcoord: 44..152
score: 7.7
NoneNo IPR availablePANTHERPTHR11670:SF40ACONITATE HYDRATASE 1coord: 44..152
score: 7.7

The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
CSPI03G00490Cla014321Watermelon (97103) v1cpiwmB262
CSPI03G00490Cla008922Watermelon (97103) v1cpiwmB200
CSPI03G00490Csa3G002530Cucumber (Chinese Long) v2cpicuB120
CSPI03G00490Csa6G507310Cucumber (Chinese Long) v2cpicuB156
CSPI03G00490MELO3C013743Melon (DHL92) v3.5.1cpimeB240
CSPI03G00490MELO3C007942Melon (DHL92) v3.5.1cpimeB258
CSPI03G00490ClCG10G000470Watermelon (Charleston Gray)cpiwcgB190
CSPI03G00490ClCG01G020600Watermelon (Charleston Gray)cpiwcgB212
CSPI03G00490Lsi07G010790Bottle gourd (USVL1VR-Ls)cpilsiB227
CSPI03G00490Lsi01G005650Bottle gourd (USVL1VR-Ls)cpilsiB164
CSPI03G00490MELO3C007942.2Melon (DHL92) v3.6.1cpimedB254
CSPI03G00490MELO3C013743.2Melon (DHL92) v3.6.1cpimedB225
CSPI03G00490CsaV3_3G000510Cucumber (Chinese Long) v3cpicucB144
CSPI03G00490CsaV3_6G047510Cucumber (Chinese Long) v3cpicucB184
CSPI03G00490Cla97C01G020080Watermelon (97103) v2cpiwmbB182
CSPI03G00490Cla97C10G185010Watermelon (97103) v2cpiwmbB184
CSPI03G00490Bhi03G000599Wax gourdcpiwgoB329
CSPI03G00490Cucsa.043950Cucumber (Gy14) v1cgycpiB025
CSPI03G00490Cucsa.322870Cucumber (Gy14) v1cgycpiB484
CSPI03G00490CmaCh03G006530Cucurbita maxima (Rimu)cmacpiB666
CSPI03G00490CmaCh07G004020Cucurbita maxima (Rimu)cmacpiB871
CSPI03G00490CmoCh07G003990Cucurbita moschata (Rifu)cmocpiB852
CSPI03G00490CmoCh03G006800Cucurbita moschata (Rifu)cmocpiB658
CSPI03G00490Cp4.1LG19g08790Cucurbita pepo (Zucchini)cpecpiB485
CSPI03G00490CsGy3G000470Cucumber (Gy14) v2cgybcpiB110
CSPI03G00490CsGy6G031720Cucumber (Gy14) v2cgybcpiB272
CSPI03G00490Carg07932Silver-seed gourdcarcpiB0671
CSPI03G00490Carg00563Silver-seed gourdcarcpiB0441
The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
CSPI03G00490CSPI06G32150Wild cucumber (PI 183967)cpicpiB133