CSPI02G22460 (gene) Wild cucumber (PI 183967)

Overview
NameCSPI02G22460
Typegene
OrganismCucumis sativus (Wild cucumber (PI 183967))
DescriptionHemoglobin-like protein
LocationChr2 : 19593060 .. 19596114 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTCATCATGAATTTATACAATTCACGAATACTTACCGGGCCACCCACCGGTATTACGCAGCCCACAGATTCGAGTTCCCTGTCCAATGACGTAGCTCCATCCGCACACCCAATCGCTCGAACGGAGAATCTCACCCCTGAGTTTCATCTTCCCTATAAAGTCCATTCGTACTTCGCCTTTGAGGCTGTCTAATCTAGCTTAACAACGCCTTGACCATCAAGTCACCGACCCCAGAAGCCGCTCCACTTCAAGGGAGAGAAGATGCAGAGTTTGCAAGATAAGGCTTCCGAGTGGAGCGGAGTGAAGCGTGAAGATGCCTTCGCCATCGACGAAGTCAATTTGTTTCAGAAGTTAGGTCTCCAGACGTTCGTTAATCTATCGACCAAATTCTATAACAGGTATGTTTCTGTTCATCAATGCAGCATGGGCGAAGTAATTTGAGTTTCGGGTTGTTTTCTTATTTTTCTCGGGAGGAAGTAAAATTATTGCCTCTGATTCCTAATCGAATTTTGTAAATTTTTGTTGATTTATTTTGAGTGAGCATGGAAATGACTGTTAATTGACGGATGATTGATAAACAAGATAATAGACGTGTCCATGATCAGTTGATGAAATGCTATTTGCTTTAGCTCTATTTCACCAGAATCAAACTGATTGTAGTCTGTACTGTTGGTTACTTTGTTTTATTGTTCCATTATTGTGAATACTAGGGTGTATGACGACGACGAGGAGTGGTTCCGATCAATTTTTGGGAATTCGAAGAAGGAGGATGCAATTCAAAATCAGTACGAGTTCTTCGTGCAGAGAATGGGAGGTCCGCCTCTATATTCTCAAAGAAAAGGCAAGTTCTTCATTTTTTTCTCGCATGTAGTTTTCCAGTATTGATTGAAAGCATAAAGTTAAACGACTCGAGGTTCGTACGTGCTGTTTTGTTCCTTCTCAAAATATATTCCATAACGACAATTCTCTTAGATTCTTTCCATCACCATGTCTCTGTTCAGGACCTCTTTCCGAAAAATTATTCTTAGCTATTTTTCTCATCGTCAATGGAAAAACTCTCATCATCTAAGCAAATCTCAAATAGGCATTCCGTTAAAAAACAAAAATTGAGAATTAATTCAAGAATGAGGGGACATGTTGAGAATTACATCTTGAAAAAATTAAGGTAGCGACTCATTATATTTAATAGTCTCCCGAAATGTTGTTTATTATCTTTTGGTTTTTTTTATTTTGTATTTCTAAAAATCAAGCTTGTAAAAACTACTTAGGACTTATGTTTCAAACTAAAAAATATTTTAACTACTTTTTTTGTTTTTTGAAAATTGATAAGAATTTACGAGTCTCAAATGTTCTTGTAAGTCTCAAATACCGTTTTACAGGTTATGAACCTTGACCTCCTACAAACTTATGTTACAAACGAATATAGTATCATTTTTTGTTGTAATTGAGCACCAGCAGTGTTCTTCATGGCTTATTAAGTGACATACACAAAACAGTAATTATGATCGTGATTCCTGTTTGTTCCCCTAATATTTACATATGGGAGTCTGTCAAACCCTCATTTGAGTTGAGGCAATCAATGAATCATGCCTTCAACATGTTCAAAACTCTTTACCCACAATTTATCATTGAGACCTCAATATGACCCTTTTGCCATGCACTGTTGATGCATTAGCATAAGAAATTTGGTTGTCATGCAAGAATGTGAGCAGTCTGCAGTTTGGTTTGTTTAAGGATAAAAAACAACGTATTAAATGATGAAAGATATGCTCGATAGTAATTTTCTCGTGTTTAAAAACACTTTTGATAGGTTCGAAATATTTCTTCCAGTCATCTTAGTTTGAGAATAACATAAAAGTTTCATCTTCAGTAAAAACAAACTATTAGTATACGAGAAAATACTCCTGTAGCGTATAGTGATTGTGTATCTTAGTGCAATGTTAGAAGTGATTTATTATCAAGCAATTTTTATCATAATGCATATAATTCAAGATACACAATCAGCCTAATGGTGGTTTAACAATTAGTTAAGTCTTTGATGACTAGTACAGTGCAACTTCACGAGTACATGAAATGGTAATCTATTTGCAGCATGATTGCTATGATCCTTACAAAAGTTGGTGACGTTTCAGGTCATCCAGCTCTTATAGGTCGACATCGACCATTCCCAGTCACGGCGGAGAGATGGTTACATCACATGCAACTAGCATTAGACGAAACCCCAGATATAGATGCAGATTCAAAAGACAGAATGATAAATTTTTTCAGGCATGTATTCTTCTCTTGTTTTGTTCCTTCTACCTTCATTCATAGACGTCTACGGCTTTTTGTTTATACATACGTATATATTATAAGCAATCACAATTCCATTGAGAACAAAACTGAAAGAATACAAGGATGTACAGATAGGCAAACTCGTAAAAAGATCCAAACTAAAACAAAAATGGTTTCCAATCTAAGGAAACAGTAAGATCTAAAAGGAAAACGCAAAAAATCCTGTACACCAAAAAATGATAAATGAAATCTAACTAGGGGCCATACCTCTTTGAGGACCTCTCAGTCTATCTAAAAATTCTATGGTTTCGCTCAAGTCCCCTATAAAACGACACAGATTCTCGTGCCAAAAGAAAAACATATACACTCTTTCTTTTTCGTGTCTTGAGTTTCCATGATTAGGTATGCTTATTTTCTGGTTGTATGCAGACACACAGCTTTCTTTCTAGTGGCTGGAGATGAAATGAAGAATCAAAACCTACAAACTCAATGCAAGCATGGTATCCAGCAATCTGCTGCGCCGTAGACATTCTAGGACAGAAACATAGCTTTTACACAAACAAATAATTTCGAAGCATAAGATTGTTGGAACTAGGTGATTCTTTGTGTATTTCCATGGCCAAGTGACCTTTAATCTTTACAATGAAGAGAGGGATCTCCTCAATTTGACTTCAAATGAAGGCAGCTTATTTTGAAATGGTTAATGTAAACCATTTTGTAATATGATGCAATTATATATTTCATTTAGGTGCCTTATGTTCAACTGTGTTGGTTTATCTCC

mRNA sequence

ATGCAGAGTTTGCAAGATAAGGCTTCCGAGTGGAGCGGAGTGAAGCGTGAAGATGCCTTCGCCATCGACGAAGTCAATTTGTTTCAGAAGTTAGGTCTCCAGACGTTCGTTAATCTATCGACCAAATTCTATAACAGGGTGTATGACGACGACGAGGAGTGGTTCCGATCAATTTTTGGGAATTCGAAGAAGGAGGATGCAATTCAAAATCAGTACGAGTTCTTCGTGCAGAGAATGGGAGGTCCGCCTCTATATTCTCAAAGAAAAGGTCATCCAGCTCTTATAGGTCGACATCGACCATTCCCAGTCACGGCGGAGAGATGGTTACATCACATGCAACTAGCATTAGACGAAACCCCAGATATAGATGCAGATTCAAAAGACAGAATGATAAATTTTTTCAGACACACAGCTTTCTTTCTAGTGGCTGGAGATGAAATGAAGAATCAAAACCTACAAACTCAATGCAAGCATGGTATCCAGCAATCTGCTGCGCCGTAG

Coding sequence (CDS)

ATGCAGAGTTTGCAAGATAAGGCTTCCGAGTGGAGCGGAGTGAAGCGTGAAGATGCCTTCGCCATCGACGAAGTCAATTTGTTTCAGAAGTTAGGTCTCCAGACGTTCGTTAATCTATCGACCAAATTCTATAACAGGGTGTATGACGACGACGAGGAGTGGTTCCGATCAATTTTTGGGAATTCGAAGAAGGAGGATGCAATTCAAAATCAGTACGAGTTCTTCGTGCAGAGAATGGGAGGTCCGCCTCTATATTCTCAAAGAAAAGGTCATCCAGCTCTTATAGGTCGACATCGACCATTCCCAGTCACGGCGGAGAGATGGTTACATCACATGCAACTAGCATTAGACGAAACCCCAGATATAGATGCAGATTCAAAAGACAGAATGATAAATTTTTTCAGACACACAGCTTTCTTTCTAGTGGCTGGAGATGAAATGAAGAATCAAAACCTACAAACTCAATGCAAGCATGGTATCCAGCAATCTGCTGCGCCGTAG
Homology
[Close]
BLAST of CSPI02G22460 vs. Swiss-Prot
Match: GLB3_ARATH (Two-on-two hemoglobin-3 OS=Arabidopsis thaliana GN=GLB3 PE=1 SV=1)

HSP 1 Score: 258.5 bits (659), Expect = 5.2e-68
Identity = 129/173 (74.57%), Postives = 141/173 (81.50%), Query Frame = 1 

Query: 1   MQSLQDKASEWSGVKREDAFAIDEVNLFQKLGLQTFVNLSTKFYNRVYDDDEEWFRSIFG 60
           MQSLQDKAS  SGV + +AFAIDE NLF KLGLQTF+NLST FY RVYDD+EEWF+SIF 
Sbjct: 1   MQSLQDKASVLSGVDQAEAFAIDESNLFDKLGLQTFINLSTNFYTRVYDDEEEWFQSIFS 60

Query: 61  NSKKEDAIQNQYEFFVQRMGGPPLYSQRKGHPALIGRHRPFPVT---AERWLHHMQLALD 120
           NS KEDAIQNQYEFFVQRMGGPPLYSQRKGHPALIGRHRPFPVT   AERWL HMQ ALD
Sbjct: 61  NSNKEDAIQNQYEFFVQRMGGPPLYSQRKGHPALIGRHRPFPVTHQAAERWLEHMQNALD 120

Query: 121 ETPDIDADSKDRMINFFRHTAFFLVAGDEMKNQN------LQTQCKHGIQQSA 165
           ++ DID DSK +M+ FFRHTAFFLVAG+E+KNQN       Q  CKH   + A
Sbjct: 121 DSVDIDQDSKIKMMKFFRHTAFFLVAGNELKNQNEKPKHKPQCACKHAANKPA 173

[Close]
BLAST of CSPI02G22460 vs. Swiss-Prot
Match: TRHBO_BACSU (Group 2 truncated hemoglobin YjbI OS=Bacillus subtilis (strain 168) GN=yjbI PE=1 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 1.9e-09
Identity = 40/127 (31.50%), Postives = 65/127 (51.18%), Query Frame = 1 

Query: 28  FQKLGLQTFVNLSTKFYNRVYDDDEEWFRSIFGNSKKEDAIQNQYEFFVQRMGGPPLYSQ 87
           ++ +G +    L   FY RV        + IF +   E A + Q +F  Q +GGPPLY++
Sbjct: 9   YEAIGEELLSQLVDTFYERVAS--HPLLKPIFPSDLTETA-RKQKQFLTQYLGGPPLYTE 68

Query: 88  RKGHPALIGRHRPFPVTAER---WLHHMQLALDETPDIDADSKDRMINFFRHTAFFLVAG 147
             GHP L  RH PFP+T ER   WL  M+ A+D    ++ + ++ +      TA  +V  
Sbjct: 69  EHGHPMLRARHLPFPITNERADAWLSCMKDAMDHV-GLEGEIREFLFGRLELTARHMVNQ 128

Query: 148 DEMKNQN 152
            E ++++
Sbjct: 129 TEAEDRS 131

[Close]
BLAST of CSPI02G22460 vs. Swiss-Prot
Match: TRHBO_MYCLE (Group 2 truncated hemoglobin GlbO OS=Mycobacterium leprae (strain TN) GN=glbO PE=3 SV=1)

HSP 1 Score: 56.2 bits (134), Expect = 3.9e-07
Identity = 35/116 (30.17%), Postives = 58/116 (50.00%), Query Frame = 1 

Query: 32  GLQTFVNLSTKFYNRVYDDDEEWFRSIFGNSKKEDAIQNQYEFFVQRMGGPPLYSQRKGH 91
           G +TF  + ++FY +V +D  E  R ++       A +    F  Q  GGP  YS ++GH
Sbjct: 11  GAETFKAIVSRFYAQVPED--EILRELYPADDLAGAEERLRMFLEQYWGGPRTYSSQRGH 70

Query: 92  PALIGRHRPFPVTA---ERWLH--HMQLALDETPDIDADSKDRMINFFRHTAFFLV 143
           P L  RH PF +TA   + WL   H  +A  ++  +D + +  ++++    A  LV
Sbjct: 71  PRLRMRHAPFRITAIERDAWLRCMHTAVASIDSHTLDNEHRRELLDYLEMAAHSLV 124

[Close]
BLAST of CSPI02G22460 vs. Swiss-Prot
Match: TRHBO_MYCBO (Group 2 truncated hemoglobin GlbO OS=Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) GN=glbO PE=3 SV=1)

HSP 1 Score: 54.3 bits (129), Expect = 1.5e-06
Identity = 33/116 (28.45%), Postives = 58/116 (50.00%), Query Frame = 1 

Query: 32  GLQTFVNLSTKFYNRVYDDDEEWFRSIFGNSKKEDAIQNQYEFFVQRMGGPPLYSQRKGH 91
           G +TF  + ++FY +V +D  E  R ++       A +    F  Q  GGP  YS+++GH
Sbjct: 11  GAKTFDAIVSRFYAQVAED--EVLRRVYPEDDLAGAEERLRMFLEQYWGGPRTYSEQRGH 70

Query: 92  PALIGRHRPFPVT---AERWLH--HMQLALDETPDIDADSKDRMINFFRHTAFFLV 143
           P L  RH PF ++    + WL   H  +A  ++  +D + +  ++++    A  LV
Sbjct: 71  PRLRMRHAPFRISLIERDAWLRCMHTAVASIDSETLDDEHRRELLDYLEMAAHSLV 124

[Close]
BLAST of CSPI02G22460 vs. Swiss-Prot
Match: TRHBO_MYCTO (Group 2 truncated hemoglobin GlbO OS=Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) GN=glbO PE=3 SV=1)

HSP 1 Score: 54.3 bits (129), Expect = 1.5e-06
Identity = 33/116 (28.45%), Postives = 58/116 (50.00%), Query Frame = 1 

Query: 32  GLQTFVNLSTKFYNRVYDDDEEWFRSIFGNSKKEDAIQNQYEFFVQRMGGPPLYSQRKGH 91
           G +TF  + ++FY +V +D  E  R ++       A +    F  Q  GGP  YS+++GH
Sbjct: 11  GAKTFDAIVSRFYAQVAED--EVLRRVYPEDDLAGAEERLRMFLEQYWGGPRTYSEQRGH 70

Query: 92  PALIGRHRPFPVT---AERWLH--HMQLALDETPDIDADSKDRMINFFRHTAFFLV 143
           P L  RH PF ++    + WL   H  +A  ++  +D + +  ++++    A  LV
Sbjct: 71  PRLRMRHAPFRISLIERDAWLRCMHTAVASIDSETLDDEHRRELLDYLEMAAHSLV 124

[Close]
BLAST of CSPI02G22460 vs. TrEMBL
Match: A0A0A0LPU3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G375770 PE=4 SV=1)

HSP 1 Score: 340.5 bits (872), Expect = 1.2e-90
Identity = 165/169 (97.63%), Postives = 165/169 (97.63%), Query Frame = 1 

Query: 1   MQSLQDKASEWSGVKREDAFAIDEVNLFQKLGLQTFVNLSTKFYNRVYDDDEEWFRSIFG 60
           MQSLQDKASEWSGVKREDAFAIDEVNLFQKLGLQTFVNLSTKFYNRVYDDDEEWFRSIFG
Sbjct: 1   MQSLQDKASEWSGVKREDAFAIDEVNLFQKLGLQTFVNLSTKFYNRVYDDDEEWFRSIFG 60

Query: 61  NSKKEDAIQNQYEFFVQRMGGPPLYSQRKGHPALIGRHRPFPVT---AERWLHHMQLALD 120
           NSKKEDAIQNQYEFFVQRMGGPPLYSQRKGHPALIGRHRPFPVT   AERWLHHMQLALD
Sbjct: 61  NSKKEDAIQNQYEFFVQRMGGPPLYSQRKGHPALIGRHRPFPVTHRAAERWLHHMQLALD 120

Query: 121 ETPDIDADSKDRMINFFRHTAFFLVAGDEMKNQNLQTQCKHGIQQSAAP 167
           ETPDIDADSKDRMI FFRHTAFFLVAGDEMKNQNLQTQCKHGIQQSAAP
Sbjct: 121 ETPDIDADSKDRMIKFFRHTAFFLVAGDEMKNQNLQTQCKHGIQQSAAP 169

[Close]
BLAST of CSPI02G22460 vs. TrEMBL
Match: Q7Y079_DATGL (2-on-2 hemoglobin OS=Datisca glomerata GN=glb3 PE=2 SV=1)

HSP 1 Score: 308.1 bits (788), Expect = 6.4e-81
Identity = 147/169 (86.98%), Postives = 156/169 (92.31%), Query Frame = 1 

Query: 1   MQSLQDKASEWSGVKREDAFAIDEVNLFQKLGLQTFVNLSTKFYNRVYDDDEEWFRSIFG 60
           MQSLQ+KAS+WSGVK+EDAFAID  NLFQKLGLQTFVNLST FYNRVYDD+EEWFRSIF 
Sbjct: 1   MQSLQEKASQWSGVKQEDAFAIDNTNLFQKLGLQTFVNLSTNFYNRVYDDEEEWFRSIFA 60

Query: 61  NSKKEDAIQNQYEFFVQRMGGPPLYSQRKGHPALIGRHRPFPVT---AERWLHHMQLALD 120
           +SKKEDAIQNQYEFFVQRMGGPPLYS+RKGHPALIGRHRPFPVT   AERWLHHM LALD
Sbjct: 61  SSKKEDAIQNQYEFFVQRMGGPPLYSRRKGHPALIGRHRPFPVTHQAAERWLHHMLLALD 120

Query: 121 ETPDIDADSKDRMINFFRHTAFFLVAGDEMKNQNLQTQCKHGIQQSAAP 167
            TPDID DSK +MINFFRHTAFFLVAGDE+KNQNLQ QCKHGIQQ+ AP
Sbjct: 121 STPDIDVDSKVKMINFFRHTAFFLVAGDELKNQNLQFQCKHGIQQTPAP 169

[Close]
BLAST of CSPI02G22460 vs. TrEMBL
Match: A9P9H2_POPTR (Putative uncharacterized protein OS=Populus trichocarpa PE=2 SV=1)

HSP 1 Score: 282.7 bits (722), Expect = 2.9e-73
Identity = 135/162 (83.33%), Postives = 144/162 (88.89%), Query Frame = 1 

Query: 1   MQSLQDKASEWSGVKREDAFAIDEVNLFQKLGLQTFVNLSTKFYNRVYDDDEEWFRSIFG 60
           MQSLQ+KASEWSGV   DAFAID  NLFQKLGLQTF+NLST FYNRVYDD+EEWFRSIF 
Sbjct: 1   MQSLQEKASEWSGVDSADAFAIDNTNLFQKLGLQTFINLSTNFYNRVYDDEEEWFRSIFV 60

Query: 61  NSKKEDAIQNQYEFFVQRMGGPPLYSQRKGHPALIGRHRPFPVT---AERWLHHMQLALD 120
           NSKKE+AIQNQYEFFVQRMGGPPLYSQRKGHPALIGRHRPFPVT   AERWLHHMQ A+D
Sbjct: 61  NSKKEEAIQNQYEFFVQRMGGPPLYSQRKGHPALIGRHRPFPVTHQAAERWLHHMQRAVD 120

Query: 121 ETPDIDADSKDRMINFFRHTAFFLVAGDEMKNQNLQTQCKHG 160
            T DID DSK +M+NFFRHTAFFLVAGDE+KNQN +  CKHG
Sbjct: 121 STSDIDDDSKIKMMNFFRHTAFFLVAGDELKNQNGRVPCKHG 162

[Close]
BLAST of CSPI02G22460 vs. TrEMBL
Match: B9H9D4_POPTR (2-on-2 hemoglobin family protein OS=Populus trichocarpa GN=POPTR_0006s26070g PE=4 SV=1)

HSP 1 Score: 282.7 bits (722), Expect = 2.9e-73
Identity = 135/162 (83.33%), Postives = 144/162 (88.89%), Query Frame = 1 

Query: 1   MQSLQDKASEWSGVKREDAFAIDEVNLFQKLGLQTFVNLSTKFYNRVYDDDEEWFRSIFG 60
           MQSLQ+KASEWSGV   DAFAID  NLFQKLGLQTF+NLST FYNRVYDD+EEWFRSIF 
Sbjct: 1   MQSLQEKASEWSGVDSADAFAIDNTNLFQKLGLQTFINLSTNFYNRVYDDEEEWFRSIFV 60

Query: 61  NSKKEDAIQNQYEFFVQRMGGPPLYSQRKGHPALIGRHRPFPVT---AERWLHHMQLALD 120
           NSKKE+AIQNQYEFFVQRMGGPPLYSQRKGHPALIGRHRPFPVT   AERWLHHMQ A+D
Sbjct: 61  NSKKEEAIQNQYEFFVQRMGGPPLYSQRKGHPALIGRHRPFPVTHQAAERWLHHMQKAVD 120

Query: 121 ETPDIDADSKDRMINFFRHTAFFLVAGDEMKNQNLQTQCKHG 160
            T DID DSK +M+NFFRHTAFFLVAGDE+KNQN +  CKHG
Sbjct: 121 STSDIDDDSKIKMMNFFRHTAFFLVAGDELKNQNGRVPCKHG 162

[Close]
BLAST of CSPI02G22460 vs. TrEMBL
Match: A2TDC3_9ROSI (Truncated hemoglobin OS=Populus tremula x Populus tremuloides GN=TrHb PE=2 SV=1)

HSP 1 Score: 282.3 bits (721), Expect = 3.7e-73
Identity = 134/162 (82.72%), Postives = 144/162 (88.89%), Query Frame = 1 

Query: 1   MQSLQDKASEWSGVKREDAFAIDEVNLFQKLGLQTFVNLSTKFYNRVYDDDEEWFRSIFG 60
           MQSLQ+KASEWSGV   DAFAID  NLFQKLGLQTF+NLST FYNRVYDD+EEWFRSIF 
Sbjct: 1   MQSLQEKASEWSGVDSADAFAIDNTNLFQKLGLQTFINLSTNFYNRVYDDEEEWFRSIFV 60

Query: 61  NSKKEDAIQNQYEFFVQRMGGPPLYSQRKGHPALIGRHRPFPVT---AERWLHHMQLALD 120
           NSKKE+AIQNQYEFFVQRMGGPPLYSQRKGHPALIGRHRPFPVT   AERWLHHM+ A+D
Sbjct: 61  NSKKEEAIQNQYEFFVQRMGGPPLYSQRKGHPALIGRHRPFPVTHQAAERWLHHMEKAVD 120

Query: 121 ETPDIDADSKDRMINFFRHTAFFLVAGDEMKNQNLQTQCKHG 160
            T DID DSK +M+NFFRHTAFFLVAGDE+KNQN +  CKHG
Sbjct: 121 STSDIDGDSKIKMMNFFRHTAFFLVAGDELKNQNGRVPCKHG 162

[Close]
BLAST of CSPI02G22460 vs. TAIR10
Match: AT4G32690.1 (AT4G32690.1 hemoglobin 3)

HSP 1 Score: 258.5 bits (659), Expect = 2.9e-69
Identity = 129/173 (74.57%), Postives = 141/173 (81.50%), Query Frame = 1 

Query: 1   MQSLQDKASEWSGVKREDAFAIDEVNLFQKLGLQTFVNLSTKFYNRVYDDDEEWFRSIFG 60
           MQSLQDKAS  SGV + +AFAIDE NLF KLGLQTF+NLST FY RVYDD+EEWF+SIF 
Sbjct: 1   MQSLQDKASVLSGVDQAEAFAIDESNLFDKLGLQTFINLSTNFYTRVYDDEEEWFQSIFS 60

Query: 61  NSKKEDAIQNQYEFFVQRMGGPPLYSQRKGHPALIGRHRPFPVT---AERWLHHMQLALD 120
           NS KEDAIQNQYEFFVQRMGGPPLYSQRKGHPALIGRHRPFPVT   AERWL HMQ ALD
Sbjct: 61  NSNKEDAIQNQYEFFVQRMGGPPLYSQRKGHPALIGRHRPFPVTHQAAERWLEHMQNALD 120

Query: 121 ETPDIDADSKDRMINFFRHTAFFLVAGDEMKNQN------LQTQCKHGIQQSA 165
           ++ DID DSK +M+ FFRHTAFFLVAG+E+KNQN       Q  CKH   + A
Sbjct: 121 DSVDIDQDSKIKMMKFFRHTAFFLVAGNELKNQNEKPKHKPQCACKHAANKPA 173

[Close]
BLAST of CSPI02G22460 vs. NCBI nr
Match: gi|449442028|ref|XP_004138784.1| (PREDICTED: two-on-two hemoglobin-3 isoform X2 [Cucumis sativus])

HSP 1 Score: 340.5 bits (872), Expect = 1.7e-90
Identity = 165/169 (97.63%), Postives = 165/169 (97.63%), Query Frame = 1 

Query: 1   MQSLQDKASEWSGVKREDAFAIDEVNLFQKLGLQTFVNLSTKFYNRVYDDDEEWFRSIFG 60
           MQSLQDKASEWSGVKREDAFAIDEVNLFQKLGLQTFVNLSTKFYNRVYDDDEEWFRSIFG
Sbjct: 1   MQSLQDKASEWSGVKREDAFAIDEVNLFQKLGLQTFVNLSTKFYNRVYDDDEEWFRSIFG 60

Query: 61  NSKKEDAIQNQYEFFVQRMGGPPLYSQRKGHPALIGRHRPFPVT---AERWLHHMQLALD 120
           NSKKEDAIQNQYEFFVQRMGGPPLYSQRKGHPALIGRHRPFPVT   AERWLHHMQLALD
Sbjct: 61  NSKKEDAIQNQYEFFVQRMGGPPLYSQRKGHPALIGRHRPFPVTHRAAERWLHHMQLALD 120

Query: 121 ETPDIDADSKDRMINFFRHTAFFLVAGDEMKNQNLQTQCKHGIQQSAAP 167
           ETPDIDADSKDRMI FFRHTAFFLVAGDEMKNQNLQTQCKHGIQQSAAP
Sbjct: 121 ETPDIDADSKDRMIKFFRHTAFFLVAGDEMKNQNLQTQCKHGIQQSAAP 169

[Close]
BLAST of CSPI02G22460 vs. NCBI nr
Match: gi|659088555|ref|XP_008445043.1| (PREDICTED: two-on-two hemoglobin-3 isoform X2 [Cucumis melo])

HSP 1 Score: 337.0 bits (863), Expect = 1.8e-89
Identity = 164/169 (97.04%), Postives = 164/169 (97.04%), Query Frame = 1 

Query: 1   MQSLQDKASEWSGVKREDAFAIDEVNLFQKLGLQTFVNLSTKFYNRVYDDDEEWFRSIFG 60
           MQSLQDKASEWSGVKREDAFAIDEVNLFQKLGLQTFV LSTKFYNRVYDDDEEWFRSIFG
Sbjct: 1   MQSLQDKASEWSGVKREDAFAIDEVNLFQKLGLQTFVTLSTKFYNRVYDDDEEWFRSIFG 60

Query: 61  NSKKEDAIQNQYEFFVQRMGGPPLYSQRKGHPALIGRHRPFPVT---AERWLHHMQLALD 120
           NSKKEDAIQNQYEFFVQRMGGPPLYSQRKGHPALIGRHRPFPVT   AERWLHHMQLALD
Sbjct: 61  NSKKEDAIQNQYEFFVQRMGGPPLYSQRKGHPALIGRHRPFPVTHRAAERWLHHMQLALD 120

Query: 121 ETPDIDADSKDRMINFFRHTAFFLVAGDEMKNQNLQTQCKHGIQQSAAP 167
           ETPDIDADSK RMINFFRHTAFFLVAGDEMKNQNLQTQCKHGIQQSAAP
Sbjct: 121 ETPDIDADSKVRMINFFRHTAFFLVAGDEMKNQNLQTQCKHGIQQSAAP 169

[Close]
BLAST of CSPI02G22460 vs. NCBI nr
Match: gi|31559437|emb|CAD33536.1| (2-on-2 hemoglobin [Datisca glomerata])

HSP 1 Score: 308.1 bits (788), Expect = 9.1e-81
Identity = 147/169 (86.98%), Postives = 156/169 (92.31%), Query Frame = 1 

Query: 1   MQSLQDKASEWSGVKREDAFAIDEVNLFQKLGLQTFVNLSTKFYNRVYDDDEEWFRSIFG 60
           MQSLQ+KAS+WSGVK+EDAFAID  NLFQKLGLQTFVNLST FYNRVYDD+EEWFRSIF 
Sbjct: 1   MQSLQEKASQWSGVKQEDAFAIDNTNLFQKLGLQTFVNLSTNFYNRVYDDEEEWFRSIFA 60

Query: 61  NSKKEDAIQNQYEFFVQRMGGPPLYSQRKGHPALIGRHRPFPVT---AERWLHHMQLALD 120
           +SKKEDAIQNQYEFFVQRMGGPPLYS+RKGHPALIGRHRPFPVT   AERWLHHM LALD
Sbjct: 61  SSKKEDAIQNQYEFFVQRMGGPPLYSRRKGHPALIGRHRPFPVTHQAAERWLHHMLLALD 120

Query: 121 ETPDIDADSKDRMINFFRHTAFFLVAGDEMKNQNLQTQCKHGIQQSAAP 167
            TPDID DSK +MINFFRHTAFFLVAGDE+KNQNLQ QCKHGIQQ+ AP
Sbjct: 121 STPDIDVDSKVKMINFFRHTAFFLVAGDELKNQNLQFQCKHGIQQTPAP 169

[Close]
BLAST of CSPI02G22460 vs. NCBI nr
Match: gi|719980371|ref|XP_010249775.1| (PREDICTED: two-on-two hemoglobin-3 [Nelumbo nucifera])

HSP 1 Score: 287.0 bits (733), Expect = 2.2e-74
Identity = 138/168 (82.14%), Postives = 144/168 (85.71%), Query Frame = 1 

Query: 1   MQSLQDKASEWSGVKREDAFAIDEVNLFQKLGLQTFVNLSTKFYNRVYDDDEEWFRSIFG 60
           MQSLQ KASEWSGV   DAFAIDE NLF+ LGLQTFV LST FYNRVYDDDEEWFRSIF 
Sbjct: 1   MQSLQQKASEWSGVSTSDAFAIDETNLFELLGLQTFVTLSTNFYNRVYDDDEEWFRSIFA 60

Query: 61  NSKKEDAIQNQYEFFVQRMGGPPLYSQRKGHPALIGRHRPFPVT---AERWLHHMQLALD 120
           NSKKEDAIQNQYEFFVQRMGGPPLYSQRKGHPALIGRHRPFPVT   AERWLHHMQ ALD
Sbjct: 61  NSKKEDAIQNQYEFFVQRMGGPPLYSQRKGHPALIGRHRPFPVTHRAAERWLHHMQQALD 120

Query: 121 ETPDIDADSKDRMINFFRHTAFFLVAGDEMKNQNLQTQCKHGIQQSAA 166
             P+IDADSK +M+NFFRHTAFFLV GDE+KNQN    CKHG  + AA
Sbjct: 121 SVPEIDADSKIKMMNFFRHTAFFLVTGDELKNQNQGVPCKHGANKPAA 168

[Close]
BLAST of CSPI02G22460 vs. NCBI nr
Match: gi|224092358|ref|XP_002309574.1| (2-on-2 hemoglobin family protein [Populus trichocarpa])

HSP 1 Score: 282.7 bits (722), Expect = 4.1e-73
Identity = 135/162 (83.33%), Postives = 144/162 (88.89%), Query Frame = 1 

Query: 1   MQSLQDKASEWSGVKREDAFAIDEVNLFQKLGLQTFVNLSTKFYNRVYDDDEEWFRSIFG 60
           MQSLQ+KASEWSGV   DAFAID  NLFQKLGLQTF+NLST FYNRVYDD+EEWFRSIF 
Sbjct: 1   MQSLQEKASEWSGVDSADAFAIDNTNLFQKLGLQTFINLSTNFYNRVYDDEEEWFRSIFV 60

Query: 61  NSKKEDAIQNQYEFFVQRMGGPPLYSQRKGHPALIGRHRPFPVT---AERWLHHMQLALD 120
           NSKKE+AIQNQYEFFVQRMGGPPLYSQRKGHPALIGRHRPFPVT   AERWLHHMQ A+D
Sbjct: 61  NSKKEEAIQNQYEFFVQRMGGPPLYSQRKGHPALIGRHRPFPVTHQAAERWLHHMQKAVD 120

Query: 121 ETPDIDADSKDRMINFFRHTAFFLVAGDEMKNQNLQTQCKHG 160
            T DID DSK +M+NFFRHTAFFLVAGDE+KNQN +  CKHG
Sbjct: 121 STSDIDDDSKIKMMNFFRHTAFFLVAGDELKNQNGRVPCKHG 162

The following BLAST results are available for this feature:
BLAST of CSPI02G22460 vs. Swiss-Prot
Match NameE-valueIdentityDescription
GLB3_ARATH5.2e-6874.57Two-on-two hemoglobin-3 OS=Arabidopsis thaliana GN=GLB3 PE=1 SV=1[more]
TRHBO_BACSU1.9e-0931.50Group 2 truncated hemoglobin YjbI OS=Bacillus subtilis (strain 168) GN=yjbI PE=1... [more]
TRHBO_MYCLE3.9e-0730.17Group 2 truncated hemoglobin GlbO OS=Mycobacterium leprae (strain TN) GN=glbO PE... [more]
TRHBO_MYCBO1.5e-0628.45Group 2 truncated hemoglobin GlbO OS=Mycobacterium bovis (strain ATCC BAA-935 / ... [more]
TRHBO_MYCTO1.5e-0628.45Group 2 truncated hemoglobin GlbO OS=Mycobacterium tuberculosis (strain CDC 1551... [more]
BLAST of CSPI02G22460 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LPU3_CUCSA1.2e-9097.63Uncharacterized protein OS=Cucumis sativus GN=Csa_2G375770 PE=4 SV=1[more]
Q7Y079_DATGL6.4e-8186.982-on-2 hemoglobin OS=Datisca glomerata GN=glb3 PE=2 SV=1[more]
A9P9H2_POPTR2.9e-7383.33Putative uncharacterized protein OS=Populus trichocarpa PE=2 SV=1[more]
B9H9D4_POPTR2.9e-7383.332-on-2 hemoglobin family protein OS=Populus trichocarpa GN=POPTR_0006s26070g PE=... [more]
A2TDC3_9ROSI3.7e-7382.72Truncated hemoglobin OS=Populus tremula x Populus tremuloides GN=TrHb PE=2 SV=1[more]
BLAST of CSPI02G22460 vs. TAIR10
Match NameE-valueIdentityDescription
AT4G32690.12.9e-6974.57 hemoglobin 3[more]
BLAST of CSPI02G22460 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|449442028|ref|XP_004138784.1|1.7e-9097.63PREDICTED: two-on-two hemoglobin-3 isoform X2 [Cucumis sativus][more]
gi|659088555|ref|XP_008445043.1|1.8e-8997.04PREDICTED: two-on-two hemoglobin-3 isoform X2 [Cucumis melo][more]
gi|31559437|emb|CAD33536.1|9.1e-8186.982-on-2 hemoglobin [Datisca glomerata][more]
gi|719980371|ref|XP_010249775.1|2.2e-7482.14PREDICTED: two-on-two hemoglobin-3 [Nelumbo nucifera][more]
gi|224092358|ref|XP_002309574.1|4.1e-7383.332-on-2 hemoglobin family protein [Populus trichocarpa][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR001486Hemoglobin_trunc
IPR009050Globin-like_sf
IPR012292Globin/Proto
Vocabulary: Molecular Function
TermDefinition
GO:0019825oxygen binding
GO:0020037heme binding
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015671 oxygen transport
biological_process GO:0009733 response to auxin
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0019825 oxygen binding
molecular_function GO:0005344 oxygen transporter activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI02G22460.1CSPI02G22460.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001486Truncated hemoglobinPFAMPF01152Bac_globincoord: 26..139
score: 2.1
IPR009050Globin-likeunknownSSF46458Globin-likecoord: 26..142
score: 4.32
IPR012292Globin/ProtoglobinGENE3DG3DSA:1.10.490.10coord: 26..144
score: 2.0
NoneNo IPR availablePANTHERPTHR10681THIOREDOXIN PEROXIDASEcoord: 4..160
score: 4.7

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
CSPI02G22460Csa2G375770Cucumber (Chinese Long) v2cpicuB065
CSPI02G22460MELO3C011160Melon (DHL92) v3.5.1cpimeB118
CSPI02G22460ClCG08G012410Watermelon (Charleston Gray)cpiwcgB168
CSPI02G22460MELO3C011160.2Melon (DHL92) v3.6.1cpimedB114
CSPI02G22460CsaV3_2G031100Cucumber (Chinese Long) v3cpicucB081
CSPI02G22460Cla97C08G155580Watermelon (97103) v2cpiwmbB162
CSPI02G22460Cucsa.161470Cucumber (Gy14) v1cgycpiB239
CSPI02G22460CmaCh05G006070Cucurbita maxima (Rimu)cmacpiB794
CSPI02G22460CmoCh05G006330Cucurbita moschata (Rifu)cmocpiB783
CSPI02G22460Cp4.1LG11g05340Cucurbita pepo (Zucchini)cpecpiB093
CSPI02G22460CsGy2G022240Cucumber (Gy14) v2cgybcpiB057
CSPI02G22460Carg08293Silver-seed gourdcarcpiB0063
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
CSPI02G22460Wax gourdcpiwgoB162
CSPI02G22460Wax gourdcpiwgoB202
CSPI02G22460Wild cucumber (PI 183967)cpicpiB066
CSPI02G22460Cucumber (Gy14) v1cgycpiB469
CSPI02G22460Cucurbita maxima (Rimu)cmacpiB013
CSPI02G22460Cucurbita maxima (Rimu)cmacpiB165
CSPI02G22460Cucurbita maxima (Rimu)cmacpiB179
CSPI02G22460Cucurbita maxima (Rimu)cmacpiB445
CSPI02G22460Cucurbita moschata (Rifu)cmocpiB003
CSPI02G22460Cucurbita moschata (Rifu)cmocpiB150
CSPI02G22460Cucurbita moschata (Rifu)cmocpiB164
CSPI02G22460Cucurbita moschata (Rifu)cmocpiB434
CSPI02G22460Cucumber (Chinese Long) v2cpicuB075
CSPI02G22460Melon (DHL92) v3.5.1cpimeB124
CSPI02G22460Watermelon (97103) v1cpiwmB112
CSPI02G22460Cucurbita pepo (Zucchini)cpecpiB540
CSPI02G22460Cucurbita pepo (Zucchini)cpecpiB775
CSPI02G22460Cucurbita pepo (Zucchini)cpecpiB811
CSPI02G22460Cucurbita pepo (Zucchini)cpecpiB826
CSPI02G22460Bottle gourd (USVL1VR-Ls)cpilsiB120
CSPI02G22460Bottle gourd (USVL1VR-Ls)cpilsiB139
CSPI02G22460Melon (DHL92) v3.6.1cpimedB119
CSPI02G22460Cucumber (Gy14) v2cgybcpiB105
CSPI02G22460Silver-seed gourdcarcpiB0664
CSPI02G22460Silver-seed gourdcarcpiB1010
CSPI02G22460Silver-seed gourdcarcpiB1014
CSPI02G22460Cucumber (Chinese Long) v3cpicucB095