CSPI02G19150 (gene) Wild cucumber (PI 183967)

Overview
NameCSPI02G19150
Typegene
OrganismCucumis sativus (Wild cucumber (PI 183967))
DescriptionCalcium-binding EF-hand family protein, putative
LocationChr2 : 17499281 .. 17499900 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CAACAACATGCACACTTTTACACAGTATATAAATCTCCATTTCAAAGAGCAAATCCTAACCACTAACATCCCAAACAAAATCTCTTCTAATTTCTTCTTGTTCTTCGTGTGCATGGGAAACAAGTACAGTTCTTTGAGTGAAGAACAGCTCGCCTGGATTTTCAAGAAACATGACATCGACAGAGACAGGAAGCTGACCAAAGAAGAGTTGACACGAGCCTTCAACTATTTGGGTTCAAGGTGGACCGCCTTGAGAGTCAAGGAAGCCATTCGCGCTGTCGATGCAAACCACGATGGCGTCATCAGTTTGGATGAGATGAGTAAGCTCATTGTGTATGTCAAAGGTTGTCGTACCTAAACAATTAATAATACTTAACTGCAACCATCTTTTACATGTCAACCTAAGTGGAACTTACTTGATGTCACCATGTAGTTGTGCCTCTCAGTTGAGTCGTAAAATACAAATGCAGCTTAGTTAGCAGTCGAAAACTTTACGTCGCAGAGTTATAAATATTAGCAAAAAGAAAAAAAATCCTTTTTATTGGATAAAAATGTAAAGTGTGATATCCACTCTCTGTCATAGATCTGAAATCTACATTTGCTAACGATGTTTAAGGGAA

mRNA sequence

ATGCACACTTTTACACAGTATATAAATCTCCATTTCAAAGAGCAAATCCTAACCACTAACATCCCAAACAAAATCTCTTCTAATTTCTTCTTGTTCTTCGTGTGCATGGGAAACAAGTACAGTTCTTTGAGTGAAGAACAGCTCGCCTGGATTTTCAAGAAACATGACATCGACAGAGACAGGAAGCTGACCAAAGAAGAGTTGACACGAGCCTTCAACTATTTGGGTTCAAGGTGGACCGCCTTGAGAGTCAAGGAAGCCATTCGCGCTGTCGATGCAAACCACGATGGCGTCATCAGTTTGGATGAGATGAGTAAGCTCATTGTGTATGTCAAAGGTTGTCGTACCTAA

Coding sequence (CDS)

ATGCACACTTTTACACAGTATATAAATCTCCATTTCAAAGAGCAAATCCTAACCACTAACATCCCAAACAAAATCTCTTCTAATTTCTTCTTGTTCTTCGTGTGCATGGGAAACAAGTACAGTTCTTTGAGTGAAGAACAGCTCGCCTGGATTTTCAAGAAACATGACATCGACAGAGACAGGAAGCTGACCAAAGAAGAGTTGACACGAGCCTTCAACTATTTGGGTTCAAGGTGGACCGCCTTGAGAGTCAAGGAAGCCATTCGCGCTGTCGATGCAAACCACGATGGCGTCATCAGTTTGGATGAGATGAGTAAGCTCATTGTGTATGTCAAAGGTTGTCGTACCTAA
Homology
[Close]
BLAST of CSPI02G19150 vs. TrEMBL
Match: A0A0A0LL93_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G357850 PE=4 SV=1)

HSP 1 Score: 167.5 bits (423), Expect = 9.4e-39
Identity = 81/81 (100.00%), Postives = 81/81 (100.00%), Query Frame = 1 

Query: 36  MGNKYSSLSEEQLAWIFKKHDIDRDRKLTKEELTRAFNYLGSRWTALRVKEAIRAVDANH 95
           MGNKYSSLSEEQLAWIFKKHDIDRDRKLTKEELTRAFNYLGSRWTALRVKEAIRAVDANH
Sbjct: 1   MGNKYSSLSEEQLAWIFKKHDIDRDRKLTKEELTRAFNYLGSRWTALRVKEAIRAVDANH 60

Query: 96  DGVISLDEMSKLIVYVKGCRT 117
           DGVISLDEMSKLIVYVKGCRT
Sbjct: 61  DGVISLDEMSKLIVYVKGCRT 81

[Close]
BLAST of CSPI02G19150 vs. TrEMBL
Match: A0A0A0LRB6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G357340 PE=4 SV=1)

HSP 1 Score: 90.9 bits (224), Expect = 1.1e-15
Identity = 46/69 (66.67%), Postives = 52/69 (75.36%), Query Frame = 1 

Query: 44  SEEQLAWIFKKHDIDRDRKLTKEELTRAFNYLGSRWTALRVKEAIRAVDANHDGVISLDE 103
           SEEQLA +FK HD D D  LTKEEL +AF YLGSR+   RV+EA+RA D N DGVIS+DE
Sbjct: 17  SEEQLANVFKNHDRDGDGHLTKEELKQAFEYLGSRFCNFRVEEALRAADTNGDGVISMDE 76

Query: 104 MSKLIVYVK 113
           M KLI Y K
Sbjct: 77  MGKLIKYAK 85

[Close]
BLAST of CSPI02G19150 vs. TrEMBL
Match: A0A0A0LP43_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G357350 PE=4 SV=1)

HSP 1 Score: 81.3 bits (199), Expect = 8.8e-13
Identity = 43/83 (51.81%), Postives = 55/83 (66.27%), Query Frame = 1 

Query: 31  LFFVCMGNKYSSLSEEQLAWIFKKHDIDRDRKLTKEELTRAFNYLGSRWTALRVKEAIRA 90
           L F C     +SL+E ++A +FK HD D D +LTKEEL  AF YLG ++T  RV +A+ A
Sbjct: 7   LIFKCFP---ASLTEFEVAKVFKNHDKDGDGRLTKEELKSAFKYLGYKFTTFRVHQALHA 66

Query: 91  VDANHDGVISLDEMSKLIVYVKG 114
            D N D VIS +EMSKLI+Y  G
Sbjct: 67  ADDNCDDVISFEEMSKLIMYTAG 86

[Close]
BLAST of CSPI02G19150 vs. TrEMBL
Match: A0A0D2V374_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_010G019100 PE=4 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 2.4e-10
Identity = 35/70 (50.00%), Postives = 50/70 (71.43%), Query Frame = 1 

Query: 42  SLSEEQLAWIFKKHDIDRDRKLTKEELTRAFNYLGSRWTALRVKEAIRAVDANHDGVISL 101
           SL+EEQL  +F+KHD++ D  L+K+EL +AF  LGSR  + RV+ ++   D N DG ISL
Sbjct: 38  SLNEEQLKAVFRKHDVNGDGCLSKDELMKAFRELGSRNPSWRVRRSLHHADGNGDGTISL 97

Query: 102 DEMSKLIVYV 112
           DE+ +L+ YV
Sbjct: 98  DELDELVKYV 107

[Close]
BLAST of CSPI02G19150 vs. TrEMBL
Match: A0A067KTQ6_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_04273 PE=4 SV=1)

HSP 1 Score: 72.8 bits (177), Expect = 3.1e-10
Identity = 36/71 (50.70%), Postives = 46/71 (64.79%), Query Frame = 1 

Query: 44  SEEQLAWIFKKHDIDRDRKLTKEELTRAFNYLGSRWTALRVKEAIRAVDANHDGVISLDE 103
           SEEQL  +FK +D D DR+L+K+EL   FN LGSR+   RV  A+   DAN DG IS +E
Sbjct: 30  SEEQLMNMFKSYDTDHDRRLSKQELKNLFNKLGSRFAGWRVWRALHHADANKDGYISEEE 89

Query: 104 MSKLIVYVKGC 115
            S L+ Y+  C
Sbjct: 90  FSDLVKYILKC 100

[Close]
BLAST of CSPI02G19150 vs. TAIR10
Match: AT2G41410.1 (AT2G41410.1 Calcium-binding EF-hand family protein)

HSP 1 Score: 47.0 bits (110), Expect = 9.4e-06
Identity = 24/69 (34.78%), Postives = 38/69 (55.07%), Query Frame = 1 

Query: 42  SLSEEQLAWIFKKHDIDRDRKLTKEELTRAFNYLG-SRWTALRVKEAIRAVDANHDGVIS 101
           S+  E+L  +F+  D+DR+ K++ EEL R F  +G  R T       I  VD N DG + 
Sbjct: 140 SVETEELREVFEIFDVDRNGKISAEELHRVFGVIGDERCTLEECMRMIATVDGNGDGFVC 199

Query: 102 LDEMSKLIV 110
            D+  +++V
Sbjct: 200 FDDFCRMMV 208

[Close]
BLAST of CSPI02G19150 vs. NCBI nr
Match: gi|659087772|ref|XP_008444628.1| (PREDICTED: probable calcium-binding protein CML18 [Cucumis melo])

HSP 1 Score: 206.1 bits (523), Expect = 3.4e-50
Identity = 103/116 (88.79%), Postives = 108/116 (93.10%), Query Frame = 1 

Query: 1   MHTFTQYINLHFKEQILTTNIPNKISSNFFLFFVCMGNKYSSLSEEQLAWIFKKHDIDRD 60
           MHTFTQYINLHF+EQ +TT IPNKISSNF LFFVCMGNKYSSLSEEQLAWIFKKHDIDRD
Sbjct: 1   MHTFTQYINLHFEEQNITTTIPNKISSNF-LFFVCMGNKYSSLSEEQLAWIFKKHDIDRD 60

Query: 61  RKLTKEELTRAFNYLGSRWTALRVKEAIRAVDANHDGVISLDEMSKLIVYVKGCRT 117
           RKLTKEEL RAF+YLGSRWTA RVK A+RAVDAN DGVIS+DEMSKLIVYVKG RT
Sbjct: 61  RKLTKEELARAFDYLGSRWTARRVKGALRAVDANGDGVISMDEMSKLIVYVKGLRT 115

[Close]
BLAST of CSPI02G19150 vs. NCBI nr
Match: gi|700207394|gb|KGN62513.1| (hypothetical protein Csa_2G357850 [Cucumis sativus])

HSP 1 Score: 167.5 bits (423), Expect = 1.3e-38
Identity = 81/81 (100.00%), Postives = 81/81 (100.00%), Query Frame = 1 

Query: 36  MGNKYSSLSEEQLAWIFKKHDIDRDRKLTKEELTRAFNYLGSRWTALRVKEAIRAVDANH 95
           MGNKYSSLSEEQLAWIFKKHDIDRDRKLTKEELTRAFNYLGSRWTALRVKEAIRAVDANH
Sbjct: 1   MGNKYSSLSEEQLAWIFKKHDIDRDRKLTKEELTRAFNYLGSRWTALRVKEAIRAVDANH 60

Query: 96  DGVISLDEMSKLIVYVKGCRT 117
           DGVISLDEMSKLIVYVKGCRT
Sbjct: 61  DGVISLDEMSKLIVYVKGCRT 81

[Close]
BLAST of CSPI02G19150 vs. NCBI nr
Match: gi|700207392|gb|KGN62511.1| (hypothetical protein Csa_2G357340 [Cucumis sativus])

HSP 1 Score: 90.9 bits (224), Expect = 1.6e-15
Identity = 46/69 (66.67%), Postives = 52/69 (75.36%), Query Frame = 1 

Query: 44  SEEQLAWIFKKHDIDRDRKLTKEELTRAFNYLGSRWTALRVKEAIRAVDANHDGVISLDE 103
           SEEQLA +FK HD D D  LTKEEL +AF YLGSR+   RV+EA+RA D N DGVIS+DE
Sbjct: 17  SEEQLANVFKNHDRDGDGHLTKEELKQAFEYLGSRFCNFRVEEALRAADTNGDGVISMDE 76

Query: 104 MSKLIVYVK 113
           M KLI Y K
Sbjct: 77  MGKLIKYAK 85

[Close]
BLAST of CSPI02G19150 vs. NCBI nr
Match: gi|700207393|gb|KGN62512.1| (hypothetical protein Csa_2G357350 [Cucumis sativus])

HSP 1 Score: 81.3 bits (199), Expect = 1.3e-12
Identity = 43/83 (51.81%), Postives = 55/83 (66.27%), Query Frame = 1 

Query: 31  LFFVCMGNKYSSLSEEQLAWIFKKHDIDRDRKLTKEELTRAFNYLGSRWTALRVKEAIRA 90
           L F C     +SL+E ++A +FK HD D D +LTKEEL  AF YLG ++T  RV +A+ A
Sbjct: 7   LIFKCFP---ASLTEFEVAKVFKNHDKDGDGRLTKEELKSAFKYLGYKFTTFRVHQALHA 66

Query: 91  VDANHDGVISLDEMSKLIVYVKG 114
            D N D VIS +EMSKLI+Y  G
Sbjct: 67  ADDNCDDVISFEEMSKLIMYTAG 86

[Close]
BLAST of CSPI02G19150 vs. NCBI nr
Match: gi|659087770|ref|XP_008444627.1| (PREDICTED: probable calcium-binding protein CML26 [Cucumis melo])

HSP 1 Score: 77.0 bits (188), Expect = 2.4e-11
Identity = 37/73 (50.68%), Postives = 52/73 (71.23%), Query Frame = 1 

Query: 41  SSLSEEQLAWIFKKHDIDRDRKLTKEELTRAFNYLGSRWTALRVKEAIRAVDANHDGVIS 100
           +SL+E ++A +F  +D D D +LTKEEL  AF YLG ++T +RV +A+ A D N D VIS
Sbjct: 14  ASLTEFEVAKVFNNYDRDGDGRLTKEELKSAFKYLGYKFTTIRVHQALHAADNNCDDVIS 73

Query: 101 LDEMSKLIVYVKG 114
            +E+SKLI+Y  G
Sbjct: 74  FEEISKLIIYTAG 86

The following BLAST results are available for this feature:
BLAST of CSPI02G19150 vs. Swiss-Prot
Match NameE-valueIdentityDescription
BLAST of CSPI02G19150 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LL93_CUCSA9.4e-39100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_2G357850 PE=4 SV=1[more]
A0A0A0LRB6_CUCSA1.1e-1566.67Uncharacterized protein OS=Cucumis sativus GN=Csa_2G357340 PE=4 SV=1[more]
A0A0A0LP43_CUCSA8.8e-1351.81Uncharacterized protein OS=Cucumis sativus GN=Csa_2G357350 PE=4 SV=1[more]
A0A0D2V374_GOSRA2.4e-1050.00Uncharacterized protein OS=Gossypium raimondii GN=B456_010G019100 PE=4 SV=1[more]
A0A067KTQ6_JATCU3.1e-1050.70Uncharacterized protein OS=Jatropha curcas GN=JCGZ_04273 PE=4 SV=1[more]
BLAST of CSPI02G19150 vs. TAIR10
Match NameE-valueIdentityDescription
AT2G41410.19.4e-0634.78 Calcium-binding EF-hand family protein[more]
BLAST of CSPI02G19150 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|659087772|ref|XP_008444628.1|3.4e-5088.79PREDICTED: probable calcium-binding protein CML18 [Cucumis melo][more]
gi|700207394|gb|KGN62513.1|1.3e-38100.00hypothetical protein Csa_2G357850 [Cucumis sativus][more]
gi|700207392|gb|KGN62511.1|1.6e-1566.67hypothetical protein Csa_2G357340 [Cucumis sativus][more]
gi|700207393|gb|KGN62512.1|1.3e-1251.81hypothetical protein Csa_2G357350 [Cucumis sativus][more]
gi|659087770|ref|XP_008444627.1|2.4e-1150.68PREDICTED: probable calcium-binding protein CML26 [Cucumis melo][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR002048EF_hand_dom
IPR011992EF-hand-dom_pair
IPR018247EF_Hand_1_Ca_BS
Vocabulary: Molecular Function
TermDefinition
GO:0005509calcium ion binding
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0005509 calcium ion binding
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI02G19150.1CSPI02G19150.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 45..107
score: 3.6
IPR002048EF-hand domainSMARTSM00054efh_1coord: 47..75
score: 2.0coord: 83..111
score: 0
IPR002048EF-hand domainPROFILEPS50222EF_HAND_2coord: 79..114
score: 11.082coord: 43..78
score: 13
IPR011992EF-hand domain pairGENE3DG3DSA:1.10.238.10coord: 32..108
score: 5.5
IPR011992EF-hand domain pairunknownSSF47473EF-handcoord: 32..109
score: 1.1
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 56..68
score: -coord: 92..104
scor
NoneNo IPR availablePANTHERPTHR10891EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEINcoord: 39..113
score: 1.7
NoneNo IPR availablePANTHERPTHR10891:SF592CALCIUM-BINDING PROTEIN CML38-RELATEDcoord: 39..113
score: 1.7

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
CSPI02G19150CmoCh09G002140Cucurbita moschata (Rifu)cmocpiB002
CSPI02G19150Cp4.1LG07g05240Cucurbita pepo (Zucchini)cpecpiB820
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
CSPI02G19150Cucumber (Chinese Long) v3cpicucB081
CSPI02G19150Cucumber (Chinese Long) v3cpicucB103
CSPI02G19150Watermelon (97103) v2cpiwmbB137
CSPI02G19150Watermelon (97103) v2cpiwmbB166
CSPI02G19150Wax gourdcpiwgoB159
CSPI02G19150Wax gourdcpiwgoB198
CSPI02G19150Wild cucumber (PI 183967)cpicpiB074
CSPI02G19150Wild cucumber (PI 183967)cpicpiB091
CSPI02G19150Cucumber (Gy14) v1cgycpiB331
CSPI02G19150Cucumber (Gy14) v1cgycpiB341
CSPI02G19150Cucurbita maxima (Rimu)cmacpiB011
CSPI02G19150Cucurbita maxima (Rimu)cmacpiB175
CSPI02G19150Cucurbita maxima (Rimu)cmacpiB439
CSPI02G19150Cucurbita maxima (Rimu)cmacpiB513
CSPI02G19150Cucurbita maxima (Rimu)cmacpiB793
CSPI02G19150Cucurbita moschata (Rifu)cmocpiB160
CSPI02G19150Cucurbita moschata (Rifu)cmocpiB428
CSPI02G19150Cucurbita moschata (Rifu)cmocpiB782
CSPI02G19150Cucumber (Chinese Long) v2cpicuB065
CSPI02G19150Cucumber (Chinese Long) v2cpicuB080
CSPI02G19150Cucumber (Chinese Long) v2cpicuB096
CSPI02G19150Melon (DHL92) v3.5.1cpimeB105
CSPI02G19150Melon (DHL92) v3.5.1cpimeB118
CSPI02G19150Melon (DHL92) v3.5.1cpimeB148
CSPI02G19150Watermelon (Charleston Gray)cpiwcgB136
CSPI02G19150Watermelon (Charleston Gray)cpiwcgB144
CSPI02G19150Watermelon (Charleston Gray)cpiwcgB172
CSPI02G19150Watermelon (97103) v1cpiwmB116
CSPI02G19150Watermelon (97103) v1cpiwmB129
CSPI02G19150Watermelon (97103) v1cpiwmB153
CSPI02G19150Cucurbita pepo (Zucchini)cpecpiB092
CSPI02G19150Cucurbita pepo (Zucchini)cpecpiB541
CSPI02G19150Cucurbita pepo (Zucchini)cpecpiB773
CSPI02G19150Bottle gourd (USVL1VR-Ls)cpilsiB114
CSPI02G19150Bottle gourd (USVL1VR-Ls)cpilsiB141
CSPI02G19150Melon (DHL92) v3.6.1cpimedB100
CSPI02G19150Melon (DHL92) v3.6.1cpimedB114
CSPI02G19150Melon (DHL92) v3.6.1cpimedB142
CSPI02G19150Cucumber (Gy14) v2cgybcpiB057
CSPI02G19150Cucumber (Gy14) v2cgybcpiB163
CSPI02G19150Cucumber (Gy14) v2cgybcpiB305
CSPI02G19150Silver-seed gourdcarcpiB0209
CSPI02G19150Silver-seed gourdcarcpiB0806