CSPI02G15290 (gene) Wild cucumber (PI 183967)

NameCSPI02G15290
Typegene
OrganismCucumis sativus (Wild cucumber (PI 183967))
DescriptionUbiquitin carboxyl-terminal hydrolase 27-like protein
LocationChr2 : 14870767 .. 14876392 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TAGATAGGAAGCATGAATCGTTCACTAATAATCTTGTATTAAGAATCTAGTCAATCACTAATAGTTGGTTCTACATCATTTACTAACACACTCGAAGTGTAATTCACATCCCAACTTATTGCATCGTATTTATAGCCGAGAGTGTACCAACCTTTTGGTCGCCGTGGCCGGCTTACCAAATGAAGCGGGTAAGCGAGAATTTCATCACCCGATTCCAACCAGCTGAGTTCTGTGTGTATGCTGCTGGGCTTTTGAGCGCCGCAGCTCTTGTTTCCTCCATTAGAGTTGGTAAACTGAACATTCTAGGTCTTTTATCCTGGACAAAGGGGCCAAGTTCATCAGAGAATCTTCGCCTACTTCCTGGATTGCAAAATCTTGGAAACAATTGCTTTTTTAATGTGATTTTACAGGTATTATTATTTTCAGCTGATGGGTTTTCTCTCTTTCTCCCTTTTGATTGGAAAGTCCGAAATGTTTTATGAATTATCTTTGTTAGCTACAGAATAGAGTTGGGTTTAGGAATTGTATTCATGTTCTTCCGAATGTGGATATTTTCTCAGGCTTTAGCTAGCTGTGTGTTTTTTCAACCTTTCCTTCAAAAGTTTGTGGAGGAATTTGAACTTCATGAGGGTATGAGGAGTGAAGCTTTACCACTTACGGTTTCTTTGGCTGCACTATTAGAAGGTATTGTCAAATTTTTTTTTTTTCTTTTGCTTTTCATTTGAAGGGTGTTTAGGCATTTGGGTCTTTACACACATGATGCTGCTGAATTTGATATATTTAGAAGCAATTCTGTTTTTGTTTCTGCTGCTCGTGTTTCAATGTAGTTTCTGAGGAATTGATGTTTTGGAATTTTTTTTTATGCAACGTCTATCCTTGACAAGCTATTATAATTCCATATAATAGTCTAATCACTAAGTAAGGTTCTATCCACTTTGGGTTGGTCTAGTCTCAATATGGACATATGTTAATAAAGGCCTAGAAGGCCTTAGAAGCAATGAGTTGCTGTGACTAGATTTAGTAGATTGCGAGTTTTTCTTTTCAACCAAATGCAGTGGGGTACGACCGTGACAGTTGTCATGCGAGAATAAATAAGTTGCTCTTGACACTCACAAATACTAAAATAAGATCACTTGCGAATCTTTTATGAAGTACTTTTTCTTAATCAAGAAATGAACTTTCCTCTGATAAGTGGTTAAGGAACTGTAAACAGTATTCATGAAGAGCAATGTTATAATTTTTTATGATGATAGATATTATAATTAATTTTGGAGAAGGTTCTTTGTTCCTGTCATTCGGTTATCAATAAAAAGCATTTCTACAGAGCTAGGTGAAATTTCTTTGGAAAGAGTTGTGTTGAGCCCAAGGAAAGTAATGCTTGCAATGGCTTATTACACTCCGAACTTCAGTCTAACAAGCCAGCAGGTGTGGAACACTGACAGTTGTATTTTTTTTATTGTTTTCAGAGTTCAACTGTGTTTGATGTCATATTGTGTTACCGTTGGTTTGTAGGATGCAGCTGAAGCGTTCCTTCATCTTCTTTCTTCATTGAAAGAAGAAATTTCAGATTTTAATTGTCCAGCTCAGTGTTCATTAACCGATGTGATGGGTTCTAATGGTAGAATTATTACTTCCTGCAATGAGAGCCCCACTGAACTTGAAAGATGGCAAGGTCACTTCCTTGTACCATTTGATGGTATTCTTGGTAGCATCTTAACTTGTCAAAGTTGTTCTTCTCAGGTTTATTCTGACTTCATAACATTAAACTTAGAGGTCTCCTAGATCATATTCTTTTTGTTATTTCCTTATACTTGCATAAACTATTGTTTGATTCCCTTGTCTCTAAACTATGAGGGGCAATTTTTTCTGCATGTTACTGGTAGTGAAACATTTTATTAAGTTGGAGAACTTTAAATCATTGTGGATTTATCCTCTTGCATTCTCATTTTTTTTTCTGTTCCAGCAGTTCATCTCTGTATGTTATTTTATTTGGTCATTTTGTTTCCTTGTTCATTAACTGACTCCTTTTCTTTTTGTAGATCTCACTGAGTTTTGAATCCTTCCACAATTTGCCTGTTTCACCAGCGCTCAGGGGTGATTCTACTATTGTATGTCTGTTTGACCATTTTACTCACCATGACTTAAATATTGTGTTAATTATTCTTGAATAACATAGGTACATAATGTTTTTCCTGTACTTATAGACCTTTTCAAATGCAATAGTAGGTTTTTGGCTGTACGTTGCTGGATTGCTTGAAACAATTCTTTGCTGCTGAAAAGGTTGAGAATTATCGTTGCAGCTATTGTTGGCATAATGCTGGGTTGAAGTATCTGTCCTCAATGGGAGCATCTGAAGTAATTCTTTTACCTAAAATTTCATTTCTTCTTTCATTCAAAGTTGTACTCCTCTTCCCTTATTTTCCACGTGTTGTTCACTATTTATTAATTTGTTATTTCTTTTTAACTGGCTTGACTTCTAAACTGCTGCAAACTCCTTACTATACGATAGCCTCATTTTTAGTCTGCGTGAGAATAGTTGCTTAATAATTGATTGGTGACTGATGTATTATTCTTCTTAAATTCTTTAGATGGAGATTGAAGAACTTATGAAGTGTGATAGGCAAGATTCTTGCGGTTGTCAGAGACTGCTTCATTTATATAAAGTTCCATGGTCAAATAAGTTTTCGTTCACCCTAAAGCAACTCAGTATTGCTCACTGTCCAAAGGTACGCCTGCAGAACTTTTTATTTATTTATGTATATTTTTAAAGGAGGAAACGAATGGTTTCATTGATGAATGAGAGGTACAAAAGAAAGTGATGAACCCTGCCACCCCAACCACCTATGATGCACAAACACAACACTATGTCAATGTTTGAGAATGTAGAGCCTACCACAAAGTGCTCCATTAAGAGTCCCAAAAATAAAGTTTGTAATTTAGGAAAAGAAAAATCCTAACCTTAAAAATCTCTCCTCTCAATTCTTACTTTTCCTCCCTCCAATTCTTCTTTCCTCCACTTCTTCCCACTTTACCTTTGATGGACTTTGTTGTTGACAGCCTTTGCCAACACTGCCCACCTGAAAGGTTTGGTTTCTTAATAATGTGTCCATGGCATGCCTACCACGTGCTTGAAATAAAAAATTATAAGGTGACATGTAATTTTGCATGTTGGACGCATGACTGGACTATGTCTTGGTGCATCCGTGGCTTGTCACATATCTGGCATTGACACTTTGTGTCCATGCTTCCTAGCCAGCCACCATATGTTGATAACACCTCCAATTAGCGACTAAAATGGGGAGACTATAAAGACAAAAGCTCAACATGCCACAAAGGGTATTTTCTAAAAAAAGAGTCATGAGAAAAGCCTATGGTTGAAAACTTGGTCACTTTTCTTTCCAAAGATTCCAGAAGAATGCTATGATGATAGCCAACCAAAGAAAGCTTCCTCCTATACTCTGAAGTGTGACCCATGAGGATGGGGTTTGGAAGATGTTTGCCAAACTTTTGTATCACATAGAAACTATACTATAACTTCTGGGATTTTAACATAGAGAAACTCGTACAAGATTGATTCCGTCAAATTTCAAGTTTTGAGCTAAAATGCCTTGTCATCATTTCTCATGTTCATGAGATTGAGAAAATTTTGAAAGGGAGTACAATTTGAGTGTCTTTATTTTTTTATTCAGTTTTTGAAACATAGAAATAAACAAGGTTAACAAGGTTAAAGATATAGGGGCATGTTTATATAAAATTATTGCTAAAACATTGGCTGGTAGATTGAAAATTGCCCTTAATACAGTTCTGCTTCACAAGGGGCTTTATAGCAAGAAGGGAAATCTTAGAGCAAGTGTTGATTGCTAACAAAACCATTGAACATCACAGGCAGAGAAACAAAGAAGGGTTACTTTTAGAGTCAATTTTGAGAAGGCTTTTTACTATGTGGATTGAAACCTTTCAGATAAAGTCTTCGAGAAGAAGGGGTTTAGGTTAATGGAGATCTTAGAACTGGAATTGCATTAGAACTGCAAATTTCTCAATCTTTGTTAATGGCCTAGAGGAAAGACTTTTGCTTCTAACGACTTTAGACAAGGTTTTTAACATTTTCTTTTTAATCCTTACTGGAGATGATCTGAGTAGACTTGTCTTAGTAGCAGGAAATAAGGGGGTGGTGGAGGGCTTTGAGGAGGGAAAGGAGTCGCTTCACTTTTTCTTTTTTTTTTGGCGGTTGAGGCAGGAAAGTTTATTTGTTTTTAACATGTTTTTCTCAATTTTTGAAACTATTTATAGCCTTGAGATTAATAGAGATCATAGGTCTCAGCTGTTGTCCTACCAAATTAAGCTCTTGGGCGTCTTTGGATGGGTGTGAGGTGAGTCAATTCCTGTCCTCTTATCTTGAGACTTCCTTTAGGGTAATTTGTAAAGTCAGGTTGTCTGGTATCTGGTCTTAGACAAGATTTAGAAATGGTTGTCTAATTGTAGATATGCCTTTTTCTCTAAAGATAGCTCACTCTTACCAACTTGGTTGGCGTTGAGTGGAGTTCTTAGTTACTTCTGCCTCTGTTCAAAAGGAAATCCCTAAAGCTTATATTTTAATTTAACTATTCCTTTATATCTGAGCTAATTGTACTTATCAATATTTATTGTATTTTCCAGATTCTGTGCATCCATCTAAAGCGTGTTTCAACGAACATGTTTGGTGATGCATTCAAACTTCAGGTATTAATTTTCTCATTAGTGGCAACTCTTGACGCCCCTAAATTAACATATTTGTTGTTGAATGTGAATGATTTCATCTTCCAAACATCCTTTTCCTGGTATCATTTTTCTGGTGAGAGCTTTATGATGAAAGTTGAGGAGTGCTACACAACTTATTGTTTTCTCCATGAAAATGATGCATTGCAATCTCATTCCTTAGGGCCATATTTCTTTCCCCCTGATTTTGGACATGTCTCCATTCATGTTGACGGGAGTGGAAATAAAAGATTTAGAAGGGCAACTTGGAGGGCAACATTCATCAAATAAAAAACCGAGCCATGGTCTTGATTTCTTCAGAACACAGTTTGATGCCAGAATGCTGAAGTACATAAATGGCCTAACGGGAGAGGATGAGTTCCCTATGAATCTAACCTCAGGTGAACCCATTTGTTATGACCGATTTAAAGGTTCTGTTGAAGAAACCAGTTCATCTCAAACTAGAGGAAGCTCAGAAACCTGGGATACAAACTTTAACTCGTGCTCCAGTTCCTCGGTAGGTCTAAAGTTTCTATTGCTTTCACAACTCAAATCATTGTACTCCGATTTGATTTTGGCGCTTGACTCAATTCAACCTTGGTGCTTGTGTATTCTCTTAATTCTACAGGAAACTAGGACTTGCCAAATAGAACCTTCAGAAACTTTTTTGTATCGAGTCGTTTCAGTCGTTCAGCATTTTGGTAAGCCAGGCGGTGGCCATTACACCGTGTATAGAAGAGTTCAAGACTACTTGCTCGGACAAGATTGCGACACACAGTCACAGGACGTCTCTACAGAATGGTTTCATGTATCAGATTCTGAAGTTCATCTCGTTTCTGAAGAAGAAGTTCTTGCAGCTGAGGCTACCATGCTTTTCTATGAGAAAATCTAG

mRNA sequence

ATGAAGCGGGTAAGCGAGAATTTCATCACCCGATTCCAACCAGCTGAGTTCTGTGTGTATGCTGCTGGGCTTTTGAGCGCCGCAGCTCTTGTTTCCTCCATTAGAGTTGGTAAACTGAACATTCTAGGTCTTTTATCCTGGACAAAGGGGCCAAGTTCATCAGAGAATCTTCGCCTACTTCCTGGATTGCAAAATCTTGGAAACAATTGCTTTTTTAATGTGATTTTACAGGCTTTAGCTAGCTGTGTGTTTTTTCAACCTTTCCTTCAAAAGTTTGTGGAGGAATTTGAACTTCATGAGGGTATGAGGAGTGAAGCTTTACCACTTACGGTTTCTTTGGCTGCACTATTAGAAGAGCTAGGTGAAATTTCTTTGGAAAGAGTTGTGTTGAGCCCAAGGAAAGTAATGCTTGCAATGGCTTATTACACTCCGAACTTCAGTCTAACAAGCCAGCAGGATGCAGCTGAAGCGTTCCTTCATCTTCTTTCTTCATTGAAAGAAGAAATTTCAGATTTTAATTGTCCAGCTCAGTGTTCATTAACCGATGTGATGGGTTCTAATGGTAGAATTATTACTTCCTGCAATGAGAGCCCCACTGAACTTGAAAGATGGCAAGGTCACTTCCTTGTACCATTTGATGGTATTCTTGGTAGCATCTTAACTTGTCAAAGTTGTTCTTCTCAGATCTCACTGAGTTTTGAATCCTTCCACAATTTGCCTGTTTCACCAGCGCTCAGGGGTGATTCTACTATTGTTTTTGGCTGTACGTTGCTGGATTGCTTGAAACAATTCTTTGCTGCTGAAAAGGTTGAGAATTATCGTTGCAGCTATTGTTGGCATAATGCTGGGTTGAAGTATCTGTCCTCAATGGGAGCATCTGAAATGGAGATTGAAGAACTTATGAAGTGTGATAGGCAAGATTCTTGCGGTTGTCAGAGACTGCTTCATTTATATAAAGTTCCATGGTCAAATAAGTTTTCGTTCACCCTAAAGCAACTCAGTATTGCTCACTGTCCAAAGATTCTGTGCATCCATCTAAAGCGTGTTTCAACGAACATGTTTGGTGATGCATTCAAACTTCAGGGCCATATTTCTTTCCCCCTGATTTTGGACATGTCTCCATTCATGTTGACGGGAGTGGAAATAAAAGATTTAGAAGGGCAACTTGGAGGGCAACATTCATCAAATAAAAAACCGAGCCATGGTCTTGATTTCTTCAGAACACAGTTTGATGCCAGAATGCTGAAGTACATAAATGGCCTAACGGGAGAGGATGAGTTCCCTATGAATCTAACCTCAGGTGAACCCATTTGTTATGACCGATTTAAAGGTTCTGTTGAAGAAACCAGTTCATCTCAAACTAGAGGAAGCTCAGAAACCTGGGATACAAACTTTAACTCGTGCTCCAGTTCCTCGGAAACTAGGACTTGCCAAATAGAACCTTCAGAAACTTTTTTGTATCGAGTCGTTTCAGTCGTTCAGCATTTTGGTAAGCCAGGCGGTGGCCATTACACCGTGTATAGAAGAGTTCAAGACTACTTGCTCGGACAAGATTGCGACACACAGTCACAGGACGTCTCTACAGAATGGTTTCATGTATCAGATTCTGAAGTTCATCTCGTTTCTGAAGAAGAAGTTCTTGCAGCTGAGGCTACCATGCTTTTCTATGAGAAAATCTAG

Coding sequence (CDS)

ATGAAGCGGGTAAGCGAGAATTTCATCACCCGATTCCAACCAGCTGAGTTCTGTGTGTATGCTGCTGGGCTTTTGAGCGCCGCAGCTCTTGTTTCCTCCATTAGAGTTGGTAAACTGAACATTCTAGGTCTTTTATCCTGGACAAAGGGGCCAAGTTCATCAGAGAATCTTCGCCTACTTCCTGGATTGCAAAATCTTGGAAACAATTGCTTTTTTAATGTGATTTTACAGGCTTTAGCTAGCTGTGTGTTTTTTCAACCTTTCCTTCAAAAGTTTGTGGAGGAATTTGAACTTCATGAGGGTATGAGGAGTGAAGCTTTACCACTTACGGTTTCTTTGGCTGCACTATTAGAAGAGCTAGGTGAAATTTCTTTGGAAAGAGTTGTGTTGAGCCCAAGGAAAGTAATGCTTGCAATGGCTTATTACACTCCGAACTTCAGTCTAACAAGCCAGCAGGATGCAGCTGAAGCGTTCCTTCATCTTCTTTCTTCATTGAAAGAAGAAATTTCAGATTTTAATTGTCCAGCTCAGTGTTCATTAACCGATGTGATGGGTTCTAATGGTAGAATTATTACTTCCTGCAATGAGAGCCCCACTGAACTTGAAAGATGGCAAGGTCACTTCCTTGTACCATTTGATGGTATTCTTGGTAGCATCTTAACTTGTCAAAGTTGTTCTTCTCAGATCTCACTGAGTTTTGAATCCTTCCACAATTTGCCTGTTTCACCAGCGCTCAGGGGTGATTCTACTATTGTTTTTGGCTGTACGTTGCTGGATTGCTTGAAACAATTCTTTGCTGCTGAAAAGGTTGAGAATTATCGTTGCAGCTATTGTTGGCATAATGCTGGGTTGAAGTATCTGTCCTCAATGGGAGCATCTGAAATGGAGATTGAAGAACTTATGAAGTGTGATAGGCAAGATTCTTGCGGTTGTCAGAGACTGCTTCATTTATATAAAGTTCCATGGTCAAATAAGTTTTCGTTCACCCTAAAGCAACTCAGTATTGCTCACTGTCCAAAGATTCTGTGCATCCATCTAAAGCGTGTTTCAACGAACATGTTTGGTGATGCATTCAAACTTCAGGGCCATATTTCTTTCCCCCTGATTTTGGACATGTCTCCATTCATGTTGACGGGAGTGGAAATAAAAGATTTAGAAGGGCAACTTGGAGGGCAACATTCATCAAATAAAAAACCGAGCCATGGTCTTGATTTCTTCAGAACACAGTTTGATGCCAGAATGCTGAAGTACATAAATGGCCTAACGGGAGAGGATGAGTTCCCTATGAATCTAACCTCAGGTGAACCCATTTGTTATGACCGATTTAAAGGTTCTGTTGAAGAAACCAGTTCATCTCAAACTAGAGGAAGCTCAGAAACCTGGGATACAAACTTTAACTCGTGCTCCAGTTCCTCGGAAACTAGGACTTGCCAAATAGAACCTTCAGAAACTTTTTTGTATCGAGTCGTTTCAGTCGTTCAGCATTTTGGTAAGCCAGGCGGTGGCCATTACACCGTGTATAGAAGAGTTCAAGACTACTTGCTCGGACAAGATTGCGACACACAGTCACAGGACGTCTCTACAGAATGGTTTCATGTATCAGATTCTGAAGTTCATCTCGTTTCTGAAGAAGAAGTTCTTGCAGCTGAGGCTACCATGCTTTTCTATGAGAAAATCTAG
BLAST of CSPI02G15290 vs. Swiss-Prot
Match: UBP27_ARATH (Ubiquitin carboxyl-terminal hydrolase 27 OS=Arabidopsis thaliana GN=UBP27 PE=2 SV=1)

HSP 1 Score: 343.2 bits (879), Expect = 5.4e-93
Identity = 197/393 (50.13%), Postives = 252/393 (64.12%), Query Frame = 1

Query: 22  AGLLSAAALVSSIRVG---KLNILGLLSWTKGPSSSENLRLLPGLQNLGNNCFFNVILQA 81
           AGLL  A  V + + G    LN L L S  +  S  ++  L+PGLQNLGNNCF NVILQA
Sbjct: 33  AGLLGVAGFVFAQQHGLFRNLNNLKLFSGREKDSGDDSF-LVPGLQNLGNNCFLNVILQA 92

Query: 82  LASCVFFQPFLQKFVEEFELH-EGMRSEALPLTVSLAALLEELGEISLERVVLSPRKVML 141
           LASC  F+ FLQ  +E+      G + E LPLT +L+ALL+ELG +   R V +PRKVM+
Sbjct: 93  LASCKDFRSFLQWVLEDARGSLAGEQEEQLPLTFALSALLQELGTVGSRRSVSNPRKVMV 152

Query: 142 AMAYYTPNFSLTSQQDAAEAFLHLLSSLKEEISDFNCPAQCS-LTDVMGSNGRIITSCNE 201
            +  Y  NF+LTSQQDAAEA LHL+SSL+EEI     P+Q S L+D++ S    + + +E
Sbjct: 153 TLTDYAKNFNLTSQQDAAEALLHLISSLQEEIVVCYRPSQSSNLSDILFSRNLRMLAPSE 212

Query: 202 SP---TELERWQGHFLVPFDGILGSILTCQSCSSQISLSFESFHNLPVSPALR-GDSTIV 261
                 EL+RW  H   PFDGILGS L C++CSSQISL F+ FH LP+SP L  G   I+
Sbjct: 213 GLHGLMELKRWHKHLRGPFDGILGSTLMCRTCSSQISLEFQFFHTLPLSPLLHHGGYNIM 272

Query: 262 FGCTLLDCLKQFFAAEKVENYRCSYCWHNAGLKYLSSMGASEMEIEELMKCDRQDSCGCQ 321
            GCTL  CLK+F   EKVENY C  CWH A LKYLS +GA+E EIE+L  C  +D C C+
Sbjct: 273 SGCTLEHCLKKFLNTEKVENYFCYRCWHGAALKYLSVIGAAETEIEKLRSCGGEDQCDCK 332

Query: 322 RLLHLYKVPWSNKFSFTLKQLSIAHCPKILCIHLKRVSTNMFGDAFKLQGHISFPLILDM 381
             LHL ++PWSN +S  LKQL IA  PK+LCI ++R S NMF + FKL GHI+FPL+L++
Sbjct: 333 TSLHLQRMPWSNSYSHILKQLIIARFPKLLCIQVQRASFNMF-EEFKLSGHIAFPLVLNL 392

Query: 382 SPFMLT--GVEIKDLEGQLGGQHSSNKKPSHGL 404
           S F  +  GV I++               +HG+
Sbjct: 393 SLFTPSSIGVNIEERIEMSSEYQKPEASKNHGM 423

BLAST of CSPI02G15290 vs. Swiss-Prot
Match: UBP30_XENTR (Ubiquitin carboxyl-terminal hydrolase 30 OS=Xenopus tropicalis GN=usp30 PE=2 SV=1)

HSP 1 Score: 119.4 bits (298), Expect = 1.3e-25
Identity = 129/514 (25.10%), Postives = 206/514 (40.08%), Query Frame = 1

Query: 59  LLPGLQNLGNNCFFNVILQALASCVFFQPFLQKFVEEFELHEGMRSEALPLTVSLAALLE 118
           L+PGL NLGN CF N +LQ LASC  F  +L  F  ++   E   +E   L+V+L  LL+
Sbjct: 83  LVPGLLNLGNTCFMNSLLQGLASCPSFIRWLADFTSKYR-QENNTTEHQHLSVTLLHLLK 142

Query: 119 EL-GEISLERVVLSPRKVMLAMAYYTPNFSLTSQQDAAEAFLHLLSSLKEEISDFNCPAQ 178
            L  +   E  VL    ++  +  +    S   +QDA E F  L SSL++E      P  
Sbjct: 143 ALCNQEGTEDEVLDASPLLEVLRAHRWQISSFEEQDAHELFHVLTSSLEDERD--RRPHV 202

Query: 179 CSLTDVMG-----SNGRIITSCNESPTELERWQGHFLVPFDGILGSILTCQSCSSQISLS 238
             L D+          R I    + P      Q     PF G L S + C+ C  Q  + 
Sbjct: 203 THLFDLDSLEFPLEPQRQIHCRTQVPIYPIPSQWKSQHPFHGRLTSNMVCKHCQHQSPMR 262

Query: 239 FESFHNLPVSPALRGDSTIVFGCTLLDCLKQFFAAEKVENYRCSYCWHNAGLKYLSSMGA 298
           +++F +L +S  +   +T     TL  CL+ F + E V++  C  C         + + A
Sbjct: 263 YDTFDSLSLSIPV---ATWGHPITLDQCLQHFISTESVKDVVCENC---------TKIHA 322

Query: 299 SEMEIEELMKCDRQDSCGCQRLLHLYKVPWSNKFSFTLKQLSIAHCPKILCIHLKRVSTN 358
           +++   + ++                     N+ +  +KQL +   P+ LCIHL+R+S +
Sbjct: 323 AQIPNSQSVE---------------------NRKTTFVKQLKLGKLPQCLCIHLQRLSWS 382

Query: 359 MFGDAFKLQGHISFPLILDMSPFMLTGVEIKDLEGQLGGQHSSNKKPSHGLDFFRTQFDA 418
             G   K   H+ F   L M  F          + ++ G  +S +  +H     +   D 
Sbjct: 383 NQGSPLKRNEHVQFSEFLAMDRF----------KYRISGCSTSKQPANHLSAAEQETTDG 442

Query: 419 R-------MLKYINGLTGEDEFPMNLTSGEPICYDRFKGSVEETSSSQTRGSSETWDTNF 478
           +        + ++NG           TS  P        + E TSSS             
Sbjct: 443 KEGGAQNPTMPFLNGACSTSYISPPFTSPLPT-------NPEWTSSS------------- 502

Query: 479 NSCSSSSETRTCQIEPSETFLYRVVSVVQHFGKPGGGHYTVYRRVQDYLLGQDCDTQSQD 538
                              +L+R+++VV H G    GH+  YRR              + 
Sbjct: 503 -------------------YLFRLMAVVVHHGDMHSGHFVTYRR-------SPAAKNQKL 504

Query: 539 VSTEWFHVSDSEVHLVSEEEVLAAEATMLFYEKI 560
            S +W  +SD  V   + +EVL++ A +LFYE+I
Sbjct: 563 TSQQWLWISDDTVRRTNFQEVLSSSAYLLFYERI 504

BLAST of CSPI02G15290 vs. Swiss-Prot
Match: UBP30_HUMAN (Ubiquitin carboxyl-terminal hydrolase 30 OS=Homo sapiens GN=USP30 PE=1 SV=1)

HSP 1 Score: 97.4 bits (241), Expect = 5.1e-19
Identity = 95/369 (25.75%), Postives = 158/369 (42.82%), Query Frame = 1

Query: 50  GPSSSENLR---LLPGLQNLGNNCFFNVILQALASCVFFQPFLQKFVEEFELHEGMRSEA 109
           GP +    R   L+PGL NLGN CF N +LQ L++C  F  +L++F  ++   +      
Sbjct: 54  GPITERKKRRKGLVPGLVNLGNTCFMNSLLQGLSACPAFIRWLEEFTSQYSRDQKEPPSH 113

Query: 110 LPLTVSLAALLEELG-EISLERVVLSPRKVMLAMAYYTPNFSLTSQQDAAEAFLHLLSSL 169
             L+++L  LL+ L  +   +  VL    ++  +  Y    S   +QDA E F  + SSL
Sbjct: 114 QYLSLTLLHLLKALSCQEVTDDEVLDASCLLDVLRMYRWQISSFEEQDAHELFHVITSSL 173

Query: 170 KEEISD-------FNCPAQCSLTDVMGSNGRIITSCNESPTELERWQGHFLVPFDGILGS 229
           ++E          F+  +    +++        T  +  PT    W+     PF G L S
Sbjct: 174 EDERDRQPRVTHLFDVHSLEQQSEITPKQITCRTRGSPHPTS-NHWKSQH--PFHGRLTS 233

Query: 230 ILTCQSCSSQISLSFESFHNLPVS-PALRGDSTIVFGCTLLDCLKQFFAAEKVENYRCSY 289
            + C+ C  Q  + F++F +L +S PA    +T     TL  CL  F ++E V +  C  
Sbjct: 234 NMVCKHCEHQSPVRFDTFDSLSLSIPA----ATWGHPLTLDHCLHHFISSESVRDVVCDN 293

Query: 290 CWHNAGLKYLSSMGASEMEIEELMKCDRQDSCGCQRLLHLYKVPWSNKFSFTLKQLSIAH 349
           C                       K + + +   +++ H        + +F +KQL +  
Sbjct: 294 C----------------------TKIEAKGTLNGEKVEH-------QRTTF-VKQLKLGK 353

Query: 350 CPKILCIHLKRVSTNMFGDAFKLQGHISFPLILDMSPFMLTGVEIKDLEGQLGGQHSS-- 405
            P+ LCIHL+R+S +  G   K   H+ F      + F++  +    L G    QH+   
Sbjct: 354 LPQCLCIHLQRLSWSSHGTPLKRHEHVQF------NEFLMMDIYKYHLLGHKPSQHNPKL 379

BLAST of CSPI02G15290 vs. Swiss-Prot
Match: UBP30_RAT (Ubiquitin carboxyl-terminal hydrolase 30 OS=Rattus norvegicus GN=Usp30 PE=3 SV=1)

HSP 1 Score: 95.9 bits (237), Expect = 1.5e-18
Identity = 93/345 (26.96%), Postives = 150/345 (43.48%), Query Frame = 1

Query: 50  GPSSSENLR---LLPGLQNLGNNCFFNVILQALASCVFFQPFLQKFVEEFELHEGMRSEA 109
           GP +    R   L+PGL NLGN CF N +LQ L++C  F  +L++F  ++   +      
Sbjct: 54  GPITERKKRRKGLVPGLVNLGNTCFMNSLLQGLSACPAFVKWLEEFTTQYSRDQQGPHTH 113

Query: 110 LPLTVSLAALLEELG-EISLERVVLSPRKVMLAMAYYTPNFSLTSQQDAAEAFLHLLSSL 169
             L+++L +LL+ L  +   E  VL    ++  +  Y    S   +QDA E F  + SSL
Sbjct: 114 QCLSLTLLSLLKALSCQEVTEEEVLDASCLLDVLRMYRWQISSFEEQDAHELFHVITSSL 173

Query: 170 KEEISDFNCPAQCSLTDVMG-------SNGRIITSCNESP-TELERWQGHFLVPFDGILG 229
           ++E      P    L DV         +  ++      SP      W+     PF G L 
Sbjct: 174 EDERD--RQPRVTHLFDVHSLEQQSEMAPRQVTCHTRGSPHPTTNPWKSQH--PFHGRLS 233

Query: 230 SILTCQSCSSQISLSFESFHNLPVS-PALRGDSTIVFGCTLLDCLKQFFAAEKVENYRCS 289
           S + C+ C  Q  + F++F +L +S PA    +T     TL  CL  F ++E V +  C 
Sbjct: 234 SNMVCKHCEHQSPVRFDTFDSLSLSIPA----ATWGHPLTLDHCLHHFISSESVRDVVCD 293

Query: 290 YCWHNAGLKYLSSMGASEMEIEELMKCDRQDSCGCQRLLHLYKVPWSNKFSFTLKQLSIA 349
            C                       K + + +   +++ H        + +F +KQL + 
Sbjct: 294 NC----------------------TKIEAKGTQNGEKVEH-------QRTTF-VKQLKLG 353

Query: 350 HCPKILCIHLKRVSTNMFGDAFKLQGHISFP--LILDMSPFMLTG 380
             P+ LCIHL+R+S +  G   K   H+ F   L++D   + L G
Sbjct: 354 KLPQCLCIHLQRLSWSSQGTPLKRHEHVQFNEFLMMDFYKYRLLG 360

BLAST of CSPI02G15290 vs. Swiss-Prot
Match: UBP30_MOUSE (Ubiquitin carboxyl-terminal hydrolase 30 OS=Mus musculus GN=Usp30 PE=1 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 1.9e-18
Identity = 93/345 (26.96%), Postives = 148/345 (42.90%), Query Frame = 1

Query: 50  GPSSSENLR---LLPGLQNLGNNCFFNVILQALASCVFFQPFLQKFVEEFELHEGMRSEA 109
           GP +    R   L+PGL NLGN CF N +LQ L++C  F  +L++F  ++   +      
Sbjct: 54  GPITERKKRRKGLVPGLVNLGNTCFMNSLLQGLSACPAFVKWLEEFTTQYSRDQQGPHTH 113

Query: 110 LPLTVSLAALLEELG-EISLERVVLSPRKVMLAMAYYTPNFSLTSQQDAAEAFLHLLSSL 169
             L+++L  LL+ L  +   E  VL    ++  +  Y    S   +QDA E F  + SSL
Sbjct: 114 QCLSLTLLNLLKALSCQEVTEDEVLDASCLLDVLRMYRWQISSFEEQDAHELFHVITSSL 173

Query: 170 KEEISDFNCPAQCSLTDVMG-------SNGRIITSCNESP-TELERWQGHFLVPFDGILG 229
           ++E      P    L DV         +  ++      SP      W+     PF G L 
Sbjct: 174 EDERD--RQPRVTHLFDVHSLEQQSEMAPRQVTCHTRGSPHPTTNHWKSQH--PFHGRLT 233

Query: 230 SILTCQSCSSQISLSFESFHNLPVS-PALRGDSTIVFGCTLLDCLKQFFAAEKVENYRCS 289
           S + C+ C  Q  + F++F +L +S PA    +T     TL  CL  F ++E V +  C 
Sbjct: 234 SNMVCKHCEHQSPVRFDTFDSLSLSIPA----ATWGHPLTLDHCLHHFISSESVRDVVCD 293

Query: 290 YCWHNAGLKYLSSMGASEMEIEELMKCDRQDSCGCQRLLHLYKVPWSNKFSFTLKQLSIA 349
            C                       K + + +   +++ H        + S  +KQL + 
Sbjct: 294 NC----------------------TKIEARGTLTGEKVEH--------QRSTFVKQLKLG 353

Query: 350 HCPKILCIHLKRVSTNMFGDAFKLQGHISFP--LILDMSPFMLTG 380
             P+ LCIHL+R+S +  G   K   H+ F   L++D   + L G
Sbjct: 354 KLPQCLCIHLQRLSWSSHGTPLKRHEHVQFNEFLMMDFYKYRLLG 360

BLAST of CSPI02G15290 vs. TrEMBL
Match: A0A0A0LJP9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G298270 PE=3 SV=1)

HSP 1 Score: 1125.5 bits (2910), Expect = 0.0e+00
Identity = 556/559 (99.46%), Postives = 557/559 (99.64%), Query Frame = 1

Query: 1   MKRVSENFITRFQPAEFCVYAAGLLSAAALVSSIRVGKLNILGLLSWTKGPSSSENLRLL 60
           MKRVSENFITRFQPAEFCVYAAGL+SAAALVSSIRVGKLNILGLLSWTKGPSSSENLRLL
Sbjct: 1   MKRVSENFITRFQPAEFCVYAAGLVSAAALVSSIRVGKLNILGLLSWTKGPSSSENLRLL 60

Query: 61  PGLQNLGNNCFFNVILQALASCVFFQPFLQKFVEEFELHEGMRSEALPLTVSLAALLEEL 120
           PGLQNLGNNCFFNVILQALASCVFFQPFLQKFVEEFELHEGMRSEALPLTVSLAALLEEL
Sbjct: 61  PGLQNLGNNCFFNVILQALASCVFFQPFLQKFVEEFELHEGMRSEALPLTVSLAALLEEL 120

Query: 121 GEISLERVVLSPRKVMLAMAYYTPNFSLTSQQDAAEAFLHLLSSLKEEISDFNCPAQCSL 180
           GEISLERVVLSPRKVMLAMAYYTPNFSLTSQQDAAEAFLHLLSSLKEEISDFN PAQCSL
Sbjct: 121 GEISLERVVLSPRKVMLAMAYYTPNFSLTSQQDAAEAFLHLLSSLKEEISDFNYPAQCSL 180

Query: 181 TDVMGSNGRIITSCNESPTELERWQGHFLVPFDGILGSILTCQSCSSQISLSFESFHNLP 240
           TDVMGSNGRIITSCNESPTELERWQGHFLVPFDGILGSILTCQSCSSQISLSFESFHNLP
Sbjct: 181 TDVMGSNGRIITSCNESPTELERWQGHFLVPFDGILGSILTCQSCSSQISLSFESFHNLP 240

Query: 241 VSPALRGDSTIVFGCTLLDCLKQFFAAEKVENYRCSYCWHNAGLKYLSSMGASEMEIEEL 300
           VSPALRGDSTIVFGCTLLDCLKQFFAAEKVENYRCSYCWHNAGLKYLSSMGASEMEIEEL
Sbjct: 241 VSPALRGDSTIVFGCTLLDCLKQFFAAEKVENYRCSYCWHNAGLKYLSSMGASEMEIEEL 300

Query: 301 MKCDRQDSCGCQRLLHLYKVPWSNKFSFTLKQLSIAHCPKILCIHLKRVSTNMFGDAFKL 360
           MKCDRQDSCGCQRLLHLYKVPWSNKFSFTLKQLSIAHCPKILCIHLKRVSTNMFGDAFKL
Sbjct: 301 MKCDRQDSCGCQRLLHLYKVPWSNKFSFTLKQLSIAHCPKILCIHLKRVSTNMFGDAFKL 360

Query: 361 QGHISFPLILDMSPFMLTGVEIKDLEGQLGGQHSSNKKPSHGLDFFRTQFDARMLKYING 420
           QGHISFPLILDMSPFMLTGVEIKDLEGQLGGQHSSNKKPSHGLDFFRTQFDARMLKYING
Sbjct: 361 QGHISFPLILDMSPFMLTGVEIKDLEGQLGGQHSSNKKPSHGLDFFRTQFDARMLKYING 420

Query: 421 LTGEDEFPMNLTSGEPICYDRFKGSVEETSSSQTRGSSETWDTNFNSCSSSSETRTCQIE 480
           LTGEDEFPMNLTSGEPICYDRFKGSVEETSSSQTRGSSETWDTNF SCSSSSETRTCQIE
Sbjct: 421 LTGEDEFPMNLTSGEPICYDRFKGSVEETSSSQTRGSSETWDTNFKSCSSSSETRTCQIE 480

Query: 481 PSETFLYRVVSVVQHFGKPGGGHYTVYRRVQDYLLGQDCDTQSQDVSTEWFHVSDSEVHL 540
           PSETFLYRVVSVVQHFGKPGGGHYTVYRRVQDYLLGQDCDTQSQDVSTEWFHVSDSEVHL
Sbjct: 481 PSETFLYRVVSVVQHFGKPGGGHYTVYRRVQDYLLGQDCDTQSQDVSTEWFHVSDSEVHL 540

Query: 541 VSEEEVLAAEATMLFYEKI 560
           VSEEEVLAAEATMLFYEKI
Sbjct: 541 VSEEEVLAAEATMLFYEKI 559

BLAST of CSPI02G15290 vs. TrEMBL
Match: M5WI21_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa023910mg PE=3 SV=1)

HSP 1 Score: 535.4 bits (1378), Expect = 8.2e-149
Identity = 296/555 (53.33%), Postives = 371/555 (66.85%), Query Frame = 1

Query: 17  FCVYAAGLLSAAALVSSIRVGKLNILGLLSWT-KGPSSSENLRLLPGLQNLGNNCFFNVI 76
           F +  AGLL  A  V +++  KL+ L  L W  K  + S  LRL+PGLQNLGNNCF NVI
Sbjct: 34  FHISVAGLLGIAGFVLALKDSKLSRLSSLDWLPKTDNGSPKLRLVPGLQNLGNNCFLNVI 93

Query: 77  LQALASCVFFQPFLQKFVEEFELHEGMRSEA--LPLTVSLAALLEELGEISLERVVLSPR 136
           LQALASC +FQPFLQK +EE+E    +  E    PL ++LAALLE+L  +   RVVLSPR
Sbjct: 94  LQALASCSYFQPFLQKVLEEYEF-SAVEDEVSGFPLAIALAALLEDLRVVGGGRVVLSPR 153

Query: 137 KVMLAMAYYTPNFSLTSQQDAAEAFLHLLSSLKEEISDFNCPAQCSLTDVMGSNGRIITS 196
           +VMLAMA+Y  NF LTSQQDAAEAFLHLLSSL+EE SD   P QCSL +V  SN RIIT 
Sbjct: 154 RVMLAMAHYIQNFDLTSQQDAAEAFLHLLSSLREEFSDCYLPNQCSLVEVFASNCRIITP 213

Query: 197 CN-ESPTELERWQGHFLVPFDGILGSILTCQSCSSQISLSFESFHNLPVSPALRGDSTIV 256
              E+ +E  RWQ H+L PFDGILGSILTCQSCSSQISL+ ESFH+LP+SP L G  TI+
Sbjct: 214 KRMENQSEQARWQQHYLGPFDGILGSILTCQSCSSQISLNLESFHSLPLSPVLDGGETIM 273

Query: 257 FGCTLLDCLKQFFAAEKVENYRCSYCWHNAGLKYLSSMGASEMEIEELMKCDRQDSCGCQ 316
            GC L DCLKQF  AE+VENY C++CWH A +KYL + G +E +IE++ +C  QDSC C+
Sbjct: 274 VGCNLEDCLKQFTIAERVENYHCNHCWHIAAVKYLFARGVNEADIEKIKRCTEQDSCDCR 333

Query: 317 RLLHLYKVPWSNKFSFTLKQLSIAHCPKILCIHLKRVSTNMFGDAFKLQGHISFPLILDM 376
           RL HL K+PWSNKFS TLKQ+S+A CPKILCIHLKRVS N+FG+  KLQG ISFPL+LD+
Sbjct: 334 RLFHLDKLPWSNKFSCTLKQISVARCPKILCIHLKRVSINVFGELVKLQGCISFPLVLDL 393

Query: 377 SPFMLTGVEIKDLEGQL--GGQHSSNKKPSHGLDFFRTQFDARMLKYINGLTG---EDEF 436
           S F  +G EI   E  +  G     ++ PS   + F  Q+D R    + GLT     +E 
Sbjct: 394 SQFTTSGSEINSWEDNMRRGQAQGQSRNPSTRSNQFTMQYDQRKHNNVYGLTRGGISEEL 453

Query: 437 PMNLTSGEPICYDRFKGSVE----ETSSSQTRGSSETWDTNFNSCSSSSETRTCQIEPSE 496
             +++        RF  + +    E+S  QT G S+T  +  +  S S E         +
Sbjct: 454 ASDIS--------RFAANAQDLPGESSFPQTAGFSKTMHSGGDQWSKSCE---------D 513

Query: 497 TFLYRVVSVVQHFGKPGGGHYTVYRRVQDYLLGQDCDTQSQDVSTEWFHVSDSEVHLVSE 556
             LY +V+VV+HFG+ G GHYTVYR V+  L  ++ D Q +  +  WF +SDSEV  VS+
Sbjct: 514 PCLYHLVAVVEHFGRAGSGHYTVYRSVRADLCEEESDDQFEAAAPCWFSISDSEVCRVSK 570

Query: 557 EEVLAAEATMLFYEK 559
           E VLAAEA++LFYEK
Sbjct: 574 EAVLAAEASLLFYEK 570

BLAST of CSPI02G15290 vs. TrEMBL
Match: A0A061FAI5_THECC (Ubiquitin carboxyl-terminal hydrolase, putative isoform 1 OS=Theobroma cacao GN=TCM_032323 PE=3 SV=1)

HSP 1 Score: 526.9 bits (1356), Expect = 2.9e-146
Identity = 292/548 (53.28%), Postives = 367/548 (66.97%), Query Frame = 1

Query: 17  FCVYAAGLLSAAALVSSI-RVGKLNILGLLSWTKGPSSSENLRLLPGLQNLGNNCFFNVI 76
           F +   GL+     V +I R GK+         K     E L L+PGLQNLGNNCF NVI
Sbjct: 35  FHISVVGLVGVVGFVLAIIRDGKVG-------EKRKKDLEKLCLVPGLQNLGNNCFLNVI 94

Query: 77  LQALASCVFFQPFLQKFVEEFELH--EGMRSEALPLTVSLAALLEELGEISLERVVLSPR 136
           LQALASC +FQ FLQK +++ +L   EG + E LPLT++L ALLEELG     RV LSP 
Sbjct: 95  LQALASCSYFQSFLQKILDKCKLSPVEG-QDENLPLTITLTALLEELGMAGERRVALSPH 154

Query: 137 KVMLAMAYYTPNFSLTSQQDAAEAFLHLLSSLKEEISDFNCPAQCSLTDVMGSN-GRIIT 196
           KVM A+A Y  NF LTSQQDA EAFLHLL+SL+EE SD   P+Q +L D   S   RI+ 
Sbjct: 155 KVMRALALYIQNFDLTSQQDAEEAFLHLLTSLREEFSDIYLPSQSTLADAFVSQTSRILI 214

Query: 197 SCNESPTELERWQGHFLVPFDGILGSILTCQSCSSQISLSFESFHNLPVSPALRGDSTIV 256
              E  +E ERWQ HFL PFDGI+GSILTCQSCSSQI L F+ FH+LP+ P L G STI+
Sbjct: 215 PQKEDQSEQERWQQHFLGPFDGIIGSILTCQSCSSQICLDFQFFHSLPLLPVLYGGSTIM 274

Query: 257 FGCTLLDCLKQFFAAEKVENYRCSYCWHNAGLKYLSSMGASEMEIEELMKCDRQDSCGCQ 316
            GCTL DCL+QF  AE++ENY CS+CWH A +KYLSS GA+EMEIE L +C  QDSC C+
Sbjct: 275 AGCTLEDCLRQFVMAEQIENYYCSHCWHIAAIKYLSSTGANEMEIERLKRCSAQDSCDCR 334

Query: 317 RLLHLYKVPWSNKFSFTLKQLSIAHCPKILCIHLKRVSTNMFGDAFKLQGHISFPLILDM 376
             L L K+PWSN FS TLKQLSIA CPKILCIHL+R S N FG+  KLQGHISFPLILD+
Sbjct: 335 SFLCLQKLPWSNNFSCTLKQLSIARCPKILCIHLQRASINEFGELIKLQGHISFPLILDL 394

Query: 377 SPFMLTGVEIKDLEG-QLGGQHSSNKKPSHGLDFFRTQFDARMLKYINGLTGEDEFPMNL 436
           SPFM + VEIK+  G Q G     N+KPS  L+    QF+  +  +I+   GE    + L
Sbjct: 395 SPFMTSEVEIKNCGGVQKGQVKLQNQKPSPCLNLINLQFET-IFNHISKPMGEVSSEI-L 454

Query: 437 TSGEPICYDRFKGSVEETSSSQTRGSSETWDTNFNSCSSSSETRTCQIEPSETFLYRVVS 496
            + +  C    +    +++ SQT G S+  +T+ +       + T ++ PSET LY++VS
Sbjct: 455 AANDFQCTSHGESFPGKSNLSQTDGCSKALNTDMHEQHGDKVSLTSKLPPSETKLYQLVS 514

Query: 497 VVQHFGKPGGGHYTVYRRVQDYLLGQDCDTQSQDVSTEWFHVSDSEVHLVSEEEVLAAEA 556
           VV+HFG+ GGGHYTVYR ++      D D  S+  + +WF +SDS+V  VSE++VLAAEA
Sbjct: 515 VVEHFGRVGGGHYTVYRIMRAKSDEADSDEYSEPATMQWFCISDSQVCRVSEKDVLAAEA 572

Query: 557 TMLFYEKI 560
           ++LFYE+I
Sbjct: 575 SLLFYERI 572

BLAST of CSPI02G15290 vs. TrEMBL
Match: A0A061F9Z2_THECC (Ubiquitin carboxyl-terminal hydrolase, putative isoform 2 OS=Theobroma cacao GN=TCM_032323 PE=3 SV=1)

HSP 1 Score: 522.3 bits (1344), Expect = 7.2e-145
Identity = 292/549 (53.19%), Postives = 367/549 (66.85%), Query Frame = 1

Query: 17  FCVYAAGLLSAAALVSSI-RVGKLNILGLLSWTKGPSSSENLRLLPGLQNLGNNCFFNVI 76
           F +   GL+     V +I R GK+         K     E L L+PGLQNLGNNCF NVI
Sbjct: 35  FHISVVGLVGVVGFVLAIIRDGKVG-------EKRKKDLEKLCLVPGLQNLGNNCFLNVI 94

Query: 77  LQALASCVFFQPFLQKFVEEFELH--EGMRSEALPLTVSLAALLEELGEISLERVVLSPR 136
           LQALASC +FQ FLQK +++ +L   EG + E LPLT++L ALLEELG     RV LSP 
Sbjct: 95  LQALASCSYFQSFLQKILDKCKLSPVEG-QDENLPLTITLTALLEELGMAGERRVALSPH 154

Query: 137 KVMLAMAYYTPNFSLTSQQDAAEAFLHLLSSLKEEISDFNCPAQCSLTDVMGSN-GRIIT 196
           KVM A+A Y  NF LTSQQDA EAFLHLL+SL+EE SD   P+Q +L D   S   RI+ 
Sbjct: 155 KVMRALALYIQNFDLTSQQDAEEAFLHLLTSLREEFSDIYLPSQSTLADAFVSQTSRILI 214

Query: 197 SCNESPTELERWQGHFLVPFDGILGSILTCQSCSSQ-ISLSFESFHNLPVSPALRGDSTI 256
              E  +E ERWQ HFL PFDGI+GSILTCQSCSSQ I L F+ FH+LP+ P L G STI
Sbjct: 215 PQKEDQSEQERWQQHFLGPFDGIIGSILTCQSCSSQQICLDFQFFHSLPLLPVLYGGSTI 274

Query: 257 VFGCTLLDCLKQFFAAEKVENYRCSYCWHNAGLKYLSSMGASEMEIEELMKCDRQDSCGC 316
           + GCTL DCL+QF  AE++ENY CS+CWH A +KYLSS GA+EMEIE L +C  QDSC C
Sbjct: 275 MAGCTLEDCLRQFVMAEQIENYYCSHCWHIAAIKYLSSTGANEMEIERLKRCSAQDSCDC 334

Query: 317 QRLLHLYKVPWSNKFSFTLKQLSIAHCPKILCIHLKRVSTNMFGDAFKLQGHISFPLILD 376
           +  L L K+PWSN FS TLKQLSIA CPKILCIHL+R S N FG+  KLQGHISFPLILD
Sbjct: 335 RSFLCLQKLPWSNNFSCTLKQLSIARCPKILCIHLQRASINEFGELIKLQGHISFPLILD 394

Query: 377 MSPFMLTGVEIKDLEG-QLGGQHSSNKKPSHGLDFFRTQFDARMLKYINGLTGEDEFPMN 436
           +SPFM + VEIK+  G Q G     N+KPS  L+    QF+  +  +I+   GE    + 
Sbjct: 395 LSPFMTSEVEIKNCGGVQKGQVKLQNQKPSPCLNLINLQFET-IFNHISKPMGEVSSEI- 454

Query: 437 LTSGEPICYDRFKGSVEETSSSQTRGSSETWDTNFNSCSSSSETRTCQIEPSETFLYRVV 496
           L + +  C    +    +++ SQT G S+  +T+ +       + T ++ PSET LY++V
Sbjct: 455 LAANDFQCTSHGESFPGKSNLSQTDGCSKALNTDMHEQHGDKVSLTSKLPPSETKLYQLV 514

Query: 497 SVVQHFGKPGGGHYTVYRRVQDYLLGQDCDTQSQDVSTEWFHVSDSEVHLVSEEEVLAAE 556
           SVV+HFG+ GGGHYTVYR ++      D D  S+  + +WF +SDS+V  VSE++VLAAE
Sbjct: 515 SVVEHFGRVGGGHYTVYRIMRAKSDEADSDEYSEPATMQWFCISDSQVCRVSEKDVLAAE 573

Query: 557 ATMLFYEKI 560
           A++LFYE+I
Sbjct: 575 ASLLFYERI 573

BLAST of CSPI02G15290 vs. TrEMBL
Match: A0A0B0N7I0_GOSAR (Ubiquitin carboxyl-terminal hydrolase 27-like protein OS=Gossypium arboreum GN=F383_14742 PE=3 SV=1)

HSP 1 Score: 500.7 bits (1288), Expect = 2.2e-138
Identity = 282/549 (51.37%), Postives = 360/549 (65.57%), Query Frame = 1

Query: 17  FCVYAAGLLSAAALVSSI-RVGKLNILGLLSWTKGPSSSENLRLLPGLQNLGNNCFFNVI 76
           F +   GL+     V +I R GKL         K     E L L+PGLQNLGNNCF NVI
Sbjct: 32  FHISVVGLVGVVGFVLAIIRDGKLG-------EKRKKHLEKLCLVPGLQNLGNNCFLNVI 91

Query: 77  LQALASCVFFQPFLQKFVEEFELHEGMRS-EALPLTVSLAALLEELGEISLERVVLSPRK 136
           LQALASC +FQ FLQK ++E E     R  E+LPLT++L+ LLEELG     RV LSP K
Sbjct: 92  LQALASCSYFQSFLQKILDECESTVVDRQGESLPLTITLSDLLEELGMAGERRVSLSPHK 151

Query: 137 VMLAMAYYTPNFSLTSQQDAAEAFLHLLSSLKEEISDFNCPAQCSLTDVMGSN-GRIIT- 196
           VM A++ Y  NF LTSQQDAAEAFLHLLSSL+EE SD   P Q +L D+  S   RI+T 
Sbjct: 152 VMSALSLYIQNFDLTSQQDAAEAFLHLLSSLREEFSDIYLPNQSTLADIFASQTSRILTP 211

Query: 197 SCNESPTELERWQGHFLVPFDGILGSILTCQSCSSQISLSFESFHNLPVSPALRGDSTIV 256
              E   E +RWQ H+L PFDGI+GSIL+C+SCS QISL F+ FH+LP+ P L G STI+
Sbjct: 212 ERKEVQNEQKRWQQHYLGPFDGIIGSILSCRSCSYQISLDFQFFHSLPLFPVLYGASTIM 271

Query: 257 FGCTLLDCLKQFFAAEKVENYRCSYCWHNAGLKYLSSMGASEMEIEELMKCDRQDSCGCQ 316
            GCTL DCL QF  AEK+ENY CS+CWH+A +K+LS  GA+E+EIE+L +C  ++SC C+
Sbjct: 272 AGCTLEDCLWQFVLAEKLENYYCSHCWHSAAIKFLSCKGANEIEIEKLKRCSAEESCDCR 331

Query: 317 RLLHLYKVPWSNKFSFTLKQLSIAHCPKILCIHLKRVSTNMFGDAFKLQGHISFPLILDM 376
               L  +PWSN FS TLKQL IA CPKILC+HL+R S N FG+  KLQGHI+FPLILD+
Sbjct: 332 SFCCLENLPWSNNFSNTLKQLCIARCPKILCLHLQRASINEFGELIKLQGHIAFPLILDL 391

Query: 377 SPFMLTGVEIKDLEGQLGGQ-HSSNKKPSHGLDFFRTQFDARMLKYINGLTGEDEFPMNL 436
           SPFM T VEI + EG   GQ    N+K    L+    Q+++ +L  I+  TGE      L
Sbjct: 392 SPFMTTEVEITNWEGVHKGQLKLQNQKTMPHLNLINAQYES-ILNRISKPTGEKVSSEIL 451

Query: 437 TSGEPICYDRFKGSVEETSS-SQTRGSSETWDTNFNSCSSSSETRTCQIEPSETFLYRVV 496
            +    C      S+ E S    T G S+  + + +   +   + T ++ PSET  YR+ 
Sbjct: 452 VANGSQCTTSPGESLPEKSKLYPTDGCSKASNIDMHEQHNDKISLTSKLPPSETKSYRLA 511

Query: 497 SVVQHFGKPGGGHYTVYRRVQDYLLGQDCDTQSQDVSTEWFHVSDSEVHLVSEEEVLAAE 556
           SVV+HFG+PGGGHYTVYR ++     +D D  S+  +T+WF +SDS+V  VSE++VLAAE
Sbjct: 512 SVVEHFGRPGGGHYTVYRSMRTKSDEEDSDEYSEPATTKWFCISDSQVCSVSEKDVLAAE 571

Query: 557 ATMLFYEKI 560
           A++LFYE+I
Sbjct: 572 ASLLFYERI 572

BLAST of CSPI02G15290 vs. TAIR10
Match: AT4G39370.3 (AT4G39370.3 ubiquitin-specific protease 27)

HSP 1 Score: 335.1 bits (858), Expect = 8.2e-92
Identity = 197/404 (48.76%), Postives = 252/404 (62.38%), Query Frame = 1

Query: 22  AGLLSAAALVSSIRVG---KLNILGLLSWTKGPSSSENLRLLPGLQNLGNNCFFNVILQA 81
           AGLL  A  V + + G    LN L L S  +  S  ++  L+PGLQNLGNNCF NVILQA
Sbjct: 33  AGLLGVAGFVFAQQHGLFRNLNNLKLFSGREKDSGDDSF-LVPGLQNLGNNCFLNVILQA 92

Query: 82  LASCVFFQPFLQKFVEEFELH-EGMRSEALPLTVSLAALLEELGEISLERVVLSPRKVML 141
           LASC  F+ FLQ  +E+      G + E LPLT +L+ALL+ELG +   R V +PRKVM+
Sbjct: 93  LASCKDFRSFLQWVLEDARGSLAGEQEEQLPLTFALSALLQELGTVGSRRSVSNPRKVMV 152

Query: 142 AMAYYTPNFSLTSQQDAAEAFLHLLSSLKEEISDFNCPAQCS-LTDVMGSNGRIITSCNE 201
            +  Y  NF+LTSQQDAAEA LHL+SSL+EEI     P+Q S L+D++ S    + + +E
Sbjct: 153 TLTDYAKNFNLTSQQDAAEALLHLISSLQEEIVVCYRPSQSSNLSDILFSRNLRMLAPSE 212

Query: 202 SP---TELERWQGHFLVPFDGILGSILTCQSCSSQISLSFESFHNLPVSPALR-GDSTIV 261
                 EL+RW  H   PFDGILGS L C++CSSQISL F+ FH LP+SP L  G   I+
Sbjct: 213 GLHGLMELKRWHKHLRGPFDGILGSTLMCRTCSSQISLEFQFFHTLPLSPLLHHGGYNIM 272

Query: 262 FGCTLLDCLKQFFAAEKVENYRCSYCWHNAGLKYLSSMGASEMEIEELMKCDRQDSCGCQ 321
            GCTL  CLK+F   EKVENY C  CWH A LKYLS +GA+E EIE+L  C  +D C C+
Sbjct: 273 SGCTLEHCLKKFLNTEKVENYFCYRCWHGAALKYLSVIGAAETEIEKLRSCGGEDQCDCK 332

Query: 322 RLLHLYKVPWSNKFSFTLKQLSIAHCPKI-----------LCIHLKRVSTNMFGDAFKLQ 381
             LHL ++PWSN +S  LKQL IA  PK+           LCI ++R S NMF + FKL 
Sbjct: 333 TSLHLQRMPWSNSYSHILKQLIIARFPKVFAYSLKLDVKLLCIQVQRASFNMF-EEFKLS 392

Query: 382 GHISFPLILDMSPFMLT--GVEIKDLEGQLGGQHSSNKKPSHGL 404
           GHI+FPL+L++S F  +  GV I++               +HG+
Sbjct: 393 GHIAFPLVLNLSLFTPSSIGVNIEERIEMSSEYQKPEASKNHGM 434

BLAST of CSPI02G15290 vs. TAIR10
Match: AT5G10790.1 (AT5G10790.1 ubiquitin-specific protease 22)

HSP 1 Score: 70.9 bits (172), Expect = 2.9e-12
Identity = 91/398 (22.86%), Postives = 151/398 (37.94%), Query Frame = 1

Query: 60  LPGLQNLGNNCFFNVILQALASCVFFQPFLQKFVEEFELHEGMRSEALPLTVSLAALLEE 119
           L GL NLG+ CF N +LQAL      + F        +L        L L   L  +   
Sbjct: 176 LRGLNNLGSTCFMNAVLQALVHAPPLRNFWLSGQHNRDLCPRRTMGLLCLPCDLDVIFSA 235

Query: 120 LGEISLERVVLSPRKVMLAMAYYTPNFSLTSQQDAAEAFLHLLSSLKEEISDFNCPAQCS 179
           +   S +R   SP  ++ +   ++ N +   QQD+ E F+ LL  + E      C  Q  
Sbjct: 236 M--FSGDRTPYSPAHLLYSWWQHSTNLATYEQQDSHEFFISLLDRIHENEGKSKCLYQ-- 295

Query: 180 LTDVMGSNGRIITSCNESPTELERWQGHFLVPFDGILGSILTCQSCSSQISLSFESFHNL 239
                               + E  Q      F G+L S +TC +C S  S +++ F ++
Sbjct: 296 --------------------DNEECQCITHKAFSGLLRSDVTCTTCGST-STTYDPFIDI 355

Query: 240 PVSPALRGDSTIVFGCTLLDCLKQFFAAEKVENYRCSYCWHNAGLKYLSSM-----GASE 299
            ++               LD +  F  A+  +N        NA +  LS        + +
Sbjct: 356 SLT---------------LDSMNGFSPADCRKNRYSGGPSVNAIMPTLSGCLDFFTRSEK 415

Query: 300 MEIEELMKCDRQDSCGCQRLLHLYKVPWSNKFSFTLKQLSIAHCPKILCIHLKRVSTNMF 359
           +  ++ + C    SCG +R               + KQ+SI   P +LC+H+KR   ++ 
Sbjct: 416 LGPDQKLNCQ---SCGEKRE--------------SSKQMSIRRLPLLLCLHVKRFEHSLT 475

Query: 360 -GDAFKLQGHISFPLILDMSPFMLT-------GVEIKDLEGQLGGQHSSNKKPSHGLDFF 419
              + K+  ++ +P  L+MSP++ +       G  I   +G+     SS+  PS   + F
Sbjct: 476 RKTSRKIDSYLQYPFRLNMSPYLSSSIIGKRFGNRIFAFDGEGEYDSSSSSSPSAEFEIF 500

Query: 420 RTQFDARMLKYINGLTGEDEFPMNLTSGEPICYDRFKG 445
                  ML+                SG  + Y R KG
Sbjct: 536 AVVTHKGMLE----------------SGHYVTYLRLKG 500

BLAST of CSPI02G15290 vs. TAIR10
Match: AT2G32780.1 (AT2G32780.1 ubiquitin-specific protease 1)

HSP 1 Score: 58.2 bits (139), Expect = 1.9e-08
Identity = 32/74 (43.24%), Postives = 46/74 (62.16%), Query Frame = 1

Query: 486  LYRVVSVVQHFGKPGGGHYTVYRRVQDYLLGQDCDTQSQDVSTEWFHVSDSEVHLVSEEE 545
            +YR+  +V+H G    GHY  Y R   +   +D DT+  + S+ W+H SDS+V   S EE
Sbjct: 1012 VYRLAGLVEHLGAMSRGHYVSYIR-GGHKERRDSDTKEPN-SSIWYHASDSQVRPASLEE 1071

Query: 546  VLAAEATMLFYEKI 560
            VL +EA +LFYE+I
Sbjct: 1072 VLRSEAYILFYERI 1083

BLAST of CSPI02G15290 vs. TAIR10
Match: AT1G32850.1 (AT1G32850.1 ubiquitin-specific protease 11)

HSP 1 Score: 57.8 bits (138), Expect = 2.5e-08
Identity = 25/80 (31.25%), Postives = 44/80 (55.00%), Query Frame = 1

Query: 480 EPSETFLYRVVSVVQHFGKPGGGHYTVYRRVQDYLLGQDCDTQSQDVSTEWFHVSDSEVH 539
           E  +++LY + ++  H+G  GGGHYT Y ++ D               T+W++  DS V 
Sbjct: 815 EDGQSYLYELYAISNHYGGLGGGHYTAYAKLMD--------------ETKWYNFDDSRVS 874

Query: 540 LVSEEEVLAAEATMLFYEKI 560
            V+E E+  + A +LFY+++
Sbjct: 875 AVNESEIKTSAAYVLFYQRV 880

BLAST of CSPI02G15290 vs. TAIR10
Match: AT1G04860.1 (AT1G04860.1 ubiquitin-specific protease 2)

HSP 1 Score: 56.6 bits (135), Expect = 5.7e-08
Identity = 31/74 (41.89%), Postives = 44/74 (59.46%), Query Frame = 1

Query: 486 LYRVVSVVQHFGKPGGGHYTVYRRVQDYLLGQDCDTQSQDVSTEWFHVSDSEVHLVSEEE 545
           +YR+  +V+H G   GGHY  Y R      G     ++   ST W++VSD+ V  VS E+
Sbjct: 890 VYRLAGLVEHSGTMRGGHYVAYVR------GGQRVKETDSSSTAWYNVSDAYVRQVSLEK 949

Query: 546 VLAAEATMLFYEKI 560
           VL +EA +LFYE+I
Sbjct: 950 VLHSEAYILFYERI 957

BLAST of CSPI02G15290 vs. NCBI nr
Match: gi|449461457|ref|XP_004148458.1| (PREDICTED: ubiquitin carboxyl-terminal hydrolase 27 [Cucumis sativus])

HSP 1 Score: 1125.5 bits (2910), Expect = 0.0e+00
Identity = 556/559 (99.46%), Postives = 557/559 (99.64%), Query Frame = 1

Query: 1   MKRVSENFITRFQPAEFCVYAAGLLSAAALVSSIRVGKLNILGLLSWTKGPSSSENLRLL 60
           MKRVSENFITRFQPAEFCVYAAGL+SAAALVSSIRVGKLNILGLLSWTKGPSSSENLRLL
Sbjct: 1   MKRVSENFITRFQPAEFCVYAAGLVSAAALVSSIRVGKLNILGLLSWTKGPSSSENLRLL 60

Query: 61  PGLQNLGNNCFFNVILQALASCVFFQPFLQKFVEEFELHEGMRSEALPLTVSLAALLEEL 120
           PGLQNLGNNCFFNVILQALASCVFFQPFLQKFVEEFELHEGMRSEALPLTVSLAALLEEL
Sbjct: 61  PGLQNLGNNCFFNVILQALASCVFFQPFLQKFVEEFELHEGMRSEALPLTVSLAALLEEL 120

Query: 121 GEISLERVVLSPRKVMLAMAYYTPNFSLTSQQDAAEAFLHLLSSLKEEISDFNCPAQCSL 180
           GEISLERVVLSPRKVMLAMAYYTPNFSLTSQQDAAEAFLHLLSSLKEEISDFN PAQCSL
Sbjct: 121 GEISLERVVLSPRKVMLAMAYYTPNFSLTSQQDAAEAFLHLLSSLKEEISDFNYPAQCSL 180

Query: 181 TDVMGSNGRIITSCNESPTELERWQGHFLVPFDGILGSILTCQSCSSQISLSFESFHNLP 240
           TDVMGSNGRIITSCNESPTELERWQGHFLVPFDGILGSILTCQSCSSQISLSFESFHNLP
Sbjct: 181 TDVMGSNGRIITSCNESPTELERWQGHFLVPFDGILGSILTCQSCSSQISLSFESFHNLP 240

Query: 241 VSPALRGDSTIVFGCTLLDCLKQFFAAEKVENYRCSYCWHNAGLKYLSSMGASEMEIEEL 300
           VSPALRGDSTIVFGCTLLDCLKQFFAAEKVENYRCSYCWHNAGLKYLSSMGASEMEIEEL
Sbjct: 241 VSPALRGDSTIVFGCTLLDCLKQFFAAEKVENYRCSYCWHNAGLKYLSSMGASEMEIEEL 300

Query: 301 MKCDRQDSCGCQRLLHLYKVPWSNKFSFTLKQLSIAHCPKILCIHLKRVSTNMFGDAFKL 360
           MKCDRQDSCGCQRLLHLYKVPWSNKFSFTLKQLSIAHCPKILCIHLKRVSTNMFGDAFKL
Sbjct: 301 MKCDRQDSCGCQRLLHLYKVPWSNKFSFTLKQLSIAHCPKILCIHLKRVSTNMFGDAFKL 360

Query: 361 QGHISFPLILDMSPFMLTGVEIKDLEGQLGGQHSSNKKPSHGLDFFRTQFDARMLKYING 420
           QGHISFPLILDMSPFMLTGVEIKDLEGQLGGQHSSNKKPSHGLDFFRTQFDARMLKYING
Sbjct: 361 QGHISFPLILDMSPFMLTGVEIKDLEGQLGGQHSSNKKPSHGLDFFRTQFDARMLKYING 420

Query: 421 LTGEDEFPMNLTSGEPICYDRFKGSVEETSSSQTRGSSETWDTNFNSCSSSSETRTCQIE 480
           LTGEDEFPMNLTSGEPICYDRFKGSVEETSSSQTRGSSETWDTNF SCSSSSETRTCQIE
Sbjct: 421 LTGEDEFPMNLTSGEPICYDRFKGSVEETSSSQTRGSSETWDTNFKSCSSSSETRTCQIE 480

Query: 481 PSETFLYRVVSVVQHFGKPGGGHYTVYRRVQDYLLGQDCDTQSQDVSTEWFHVSDSEVHL 540
           PSETFLYRVVSVVQHFGKPGGGHYTVYRRVQDYLLGQDCDTQSQDVSTEWFHVSDSEVHL
Sbjct: 481 PSETFLYRVVSVVQHFGKPGGGHYTVYRRVQDYLLGQDCDTQSQDVSTEWFHVSDSEVHL 540

Query: 541 VSEEEVLAAEATMLFYEKI 560
           VSEEEVLAAEATMLFYEKI
Sbjct: 541 VSEEEVLAAEATMLFYEKI 559

BLAST of CSPI02G15290 vs. NCBI nr
Match: gi|659116134|ref|XP_008457921.1| (PREDICTED: ubiquitin carboxyl-terminal hydrolase 27 [Cucumis melo])

HSP 1 Score: 1076.6 bits (2783), Expect = 0.0e+00
Identity = 537/559 (96.06%), Postives = 542/559 (96.96%), Query Frame = 1

Query: 1   MKRVSENFITRFQPAEFCVYAAGLLSAAALVSSIRVGKLNILGLLSWTKGPSSSENLRLL 60
           MKRV+EN ITRFQPAEFCVY AGLLSAAALVS+IR GKLNILGLLSW K PSSSENLRLL
Sbjct: 1   MKRVNENLITRFQPAEFCVYVAGLLSAAALVSAIRDGKLNILGLLSWAKEPSSSENLRLL 60

Query: 61  PGLQNLGNNCFFNVILQALASCVFFQPFLQKFVEEFELHEGMRSEALPLTVSLAALLEEL 120
           PGLQNLGNNCFFNVILQALASCVFFQPFLQKFVEEFELHEGMR EALPLTVSLAALLEEL
Sbjct: 61  PGLQNLGNNCFFNVILQALASCVFFQPFLQKFVEEFELHEGMRREALPLTVSLAALLEEL 120

Query: 121 GEISLERVVLSPRKVMLAMAYYTPNFSLTSQQDAAEAFLHLLSSLKEEISDFNCPAQCSL 180
           G +SLERVVLSPRKVMLAMAYYTPNFSLTSQQDAAEAFLHLLSSLKEEISDFN PAQCSL
Sbjct: 121 GAVSLERVVLSPRKVMLAMAYYTPNFSLTSQQDAAEAFLHLLSSLKEEISDFNYPAQCSL 180

Query: 181 TDVMGSNGRIITSCNESPTELERWQGHFLVPFDGILGSILTCQSCSSQISLSFESFHNLP 240
           TDVMGSNGRIITSC ESPTELERWQGHFLVPFDGILGSILTCQSCSSQISLSFESFHNLP
Sbjct: 181 TDVMGSNGRIITSCTESPTELERWQGHFLVPFDGILGSILTCQSCSSQISLSFESFHNLP 240

Query: 241 VSPALRGDSTIVFGCTLLDCLKQFFAAEKVENYRCSYCWHNAGLKYLSSMGASEMEIEEL 300
           VSP LRGDSTIVFGCTLLDCLKQFFAAEKVENYRCSYCWHNAGLKYLSSMGASEMEIEEL
Sbjct: 241 VSPVLRGDSTIVFGCTLLDCLKQFFAAEKVENYRCSYCWHNAGLKYLSSMGASEMEIEEL 300

Query: 301 MKCDRQDSCGCQRLLHLYKVPWSNKFSFTLKQLSIAHCPKILCIHLKRVSTNMFGDAFKL 360
           MKCDRQDSCGCQRLLHLYKVPWSNKFSFTLKQLSIAHCPKILCIHLKRVSTNMFGD FKL
Sbjct: 301 MKCDRQDSCGCQRLLHLYKVPWSNKFSFTLKQLSIAHCPKILCIHLKRVSTNMFGDTFKL 360

Query: 361 QGHISFPLILDMSPFMLTGVEIKDLEGQLGGQHSSNKKPSHGLDFFRTQFDARMLKYING 420
           QGHISFPLILDMSPFMLTGVEIKDL GQLGGQHSSNKK SHGLDFFRTQFDARMLKYING
Sbjct: 361 QGHISFPLILDMSPFMLTGVEIKDL-GQLGGQHSSNKKSSHGLDFFRTQFDARMLKYING 420

Query: 421 LTGEDEFPMNLTSGEPICYDRFKGSVEETSSSQTRGSSETWDTNFNSCSSSSETRTCQIE 480
           LTGEDEFP+ LTSGE ICYDR KGSVEETSSSQTRGSSETWDTNFNSCSSSSETRTCQIE
Sbjct: 421 LTGEDEFPI-LTSGESICYDRVKGSVEETSSSQTRGSSETWDTNFNSCSSSSETRTCQIE 480

Query: 481 PSETFLYRVVSVVQHFGKPGGGHYTVYRRVQDYLLGQDCDTQSQDVSTEWFHVSDSEVHL 540
           PSET LYRVVSVVQHFGKPGGGHYTVYRRVQDYLLGQDCDTQSQ+VSTEWFHVSDSEVHL
Sbjct: 481 PSETCLYRVVSVVQHFGKPGGGHYTVYRRVQDYLLGQDCDTQSQEVSTEWFHVSDSEVHL 540

Query: 541 VSEEEVLAAEATMLFYEKI 560
           VSEEEVLAAEATMLFYEKI
Sbjct: 541 VSEEEVLAAEATMLFYEKI 557

BLAST of CSPI02G15290 vs. NCBI nr
Match: gi|595838648|ref|XP_007207694.1| (hypothetical protein PRUPE_ppa023910mg [Prunus persica])

HSP 1 Score: 535.4 bits (1378), Expect = 1.2e-148
Identity = 296/555 (53.33%), Postives = 371/555 (66.85%), Query Frame = 1

Query: 17  FCVYAAGLLSAAALVSSIRVGKLNILGLLSWT-KGPSSSENLRLLPGLQNLGNNCFFNVI 76
           F +  AGLL  A  V +++  KL+ L  L W  K  + S  LRL+PGLQNLGNNCF NVI
Sbjct: 34  FHISVAGLLGIAGFVLALKDSKLSRLSSLDWLPKTDNGSPKLRLVPGLQNLGNNCFLNVI 93

Query: 77  LQALASCVFFQPFLQKFVEEFELHEGMRSEA--LPLTVSLAALLEELGEISLERVVLSPR 136
           LQALASC +FQPFLQK +EE+E    +  E    PL ++LAALLE+L  +   RVVLSPR
Sbjct: 94  LQALASCSYFQPFLQKVLEEYEF-SAVEDEVSGFPLAIALAALLEDLRVVGGGRVVLSPR 153

Query: 137 KVMLAMAYYTPNFSLTSQQDAAEAFLHLLSSLKEEISDFNCPAQCSLTDVMGSNGRIITS 196
           +VMLAMA+Y  NF LTSQQDAAEAFLHLLSSL+EE SD   P QCSL +V  SN RIIT 
Sbjct: 154 RVMLAMAHYIQNFDLTSQQDAAEAFLHLLSSLREEFSDCYLPNQCSLVEVFASNCRIITP 213

Query: 197 CN-ESPTELERWQGHFLVPFDGILGSILTCQSCSSQISLSFESFHNLPVSPALRGDSTIV 256
              E+ +E  RWQ H+L PFDGILGSILTCQSCSSQISL+ ESFH+LP+SP L G  TI+
Sbjct: 214 KRMENQSEQARWQQHYLGPFDGILGSILTCQSCSSQISLNLESFHSLPLSPVLDGGETIM 273

Query: 257 FGCTLLDCLKQFFAAEKVENYRCSYCWHNAGLKYLSSMGASEMEIEELMKCDRQDSCGCQ 316
            GC L DCLKQF  AE+VENY C++CWH A +KYL + G +E +IE++ +C  QDSC C+
Sbjct: 274 VGCNLEDCLKQFTIAERVENYHCNHCWHIAAVKYLFARGVNEADIEKIKRCTEQDSCDCR 333

Query: 317 RLLHLYKVPWSNKFSFTLKQLSIAHCPKILCIHLKRVSTNMFGDAFKLQGHISFPLILDM 376
           RL HL K+PWSNKFS TLKQ+S+A CPKILCIHLKRVS N+FG+  KLQG ISFPL+LD+
Sbjct: 334 RLFHLDKLPWSNKFSCTLKQISVARCPKILCIHLKRVSINVFGELVKLQGCISFPLVLDL 393

Query: 377 SPFMLTGVEIKDLEGQL--GGQHSSNKKPSHGLDFFRTQFDARMLKYINGLTG---EDEF 436
           S F  +G EI   E  +  G     ++ PS   + F  Q+D R    + GLT     +E 
Sbjct: 394 SQFTTSGSEINSWEDNMRRGQAQGQSRNPSTRSNQFTMQYDQRKHNNVYGLTRGGISEEL 453

Query: 437 PMNLTSGEPICYDRFKGSVE----ETSSSQTRGSSETWDTNFNSCSSSSETRTCQIEPSE 496
             +++        RF  + +    E+S  QT G S+T  +  +  S S E         +
Sbjct: 454 ASDIS--------RFAANAQDLPGESSFPQTAGFSKTMHSGGDQWSKSCE---------D 513

Query: 497 TFLYRVVSVVQHFGKPGGGHYTVYRRVQDYLLGQDCDTQSQDVSTEWFHVSDSEVHLVSE 556
             LY +V+VV+HFG+ G GHYTVYR V+  L  ++ D Q +  +  WF +SDSEV  VS+
Sbjct: 514 PCLYHLVAVVEHFGRAGSGHYTVYRSVRADLCEEESDDQFEAAAPCWFSISDSEVCRVSK 570

Query: 557 EEVLAAEATMLFYEK 559
           E VLAAEA++LFYEK
Sbjct: 574 EAVLAAEASLLFYEK 570

BLAST of CSPI02G15290 vs. NCBI nr
Match: gi|694430671|ref|XP_009342800.1| (PREDICTED: ubiquitin carboxyl-terminal hydrolase 27-like [Pyrus x bretschneideri])

HSP 1 Score: 532.3 bits (1370), Expect = 1.0e-147
Identity = 296/565 (52.39%), Postives = 370/565 (65.49%), Query Frame = 1

Query: 6   ENFITRFQPAEFCVYAAGLLSAAALVSSIRVGKLNILGLL--SWT--KGPSSSENLRLLP 65
           + F+T    +     AA L  A   V ++R  K  IL  L   W      SS E L L+P
Sbjct: 18  QGFMTLSGSSSISSVAAILGVAGGFVLALRGSKFGILNTLHLEWLLHTETSSPEQLPLVP 77

Query: 66  GLQNLGNNCFFNVILQALASCVFFQPFLQKFVEEFELHE-GMRSEALPLTVSLAALLEEL 125
           GLQNLGNNCF NVILQALASC +FQPF QK +EE+E    G R +  PL ++LA LLE+L
Sbjct: 78  GLQNLGNNCFLNVILQALASCSYFQPFPQKVLEEYEFSAAGDRVDGFPLAIALATLLEDL 137

Query: 126 GEISLERVVLSPRKVMLAMAYYTPNFSLTSQQDAAEAFLHLLSSLKEEISDFNCPAQCSL 185
             +   RVVLSPRKVMLAMA Y  NF LTSQQDAAEAFLHLLSSL+EE SD   P QCSL
Sbjct: 138 RIVGGGRVVLSPRKVMLAMADYIQNFDLTSQQDAAEAFLHLLSSLREEFSDCYLPNQCSL 197

Query: 186 TDVMGSNGRIITSCN-ESPTELERWQGHFLVPFDGILGSILTCQSCSSQISLSFESFHNL 245
            ++  SN RIIT  + E+ ++  RWQ H+L PFDGILGSILTCQSCSSQISL+FES H+L
Sbjct: 198 AELCASNCRIITPKSMENQSKQARWQQHYLGPFDGILGSILTCQSCSSQISLNFESCHSL 257

Query: 246 PVSPALRGDSTIVFGCTLLDCLKQFFAAEKVENYRCSYCWHNAGLKYLSSMGASEMEIEE 305
           P+SP L G  TI+ GCTL DCL+QF AAE+VENY C++CWH A +KYL S+G +E EIE+
Sbjct: 258 PLSPVLDGGGTIMVGCTLEDCLEQFTAAEQVENYHCNHCWHIAAVKYLFSLGVNEEEIEK 317

Query: 306 LMKCDRQDSCGCQRLLHLYKVPWSNKFSFTLKQLSIAHCPKILCIHLKRVSTNMFGDAFK 365
           + +C  QDSC C+   HL+K+PWSN+FS+TLKQ+SIA CPKILCIHLKRVS N+FG+  K
Sbjct: 318 IKRCPEQDSCDCRSHFHLHKLPWSNRFSYTLKQISIARCPKILCIHLKRVSVNIFGELVK 377

Query: 366 LQGHISFPLILDMSPFMLTGVEIKDLEGQLGGQHSSNKKPSHGLDFFRT----QFDARML 425
           LQGHISFPL+LD+  F  T V IK  E  +  +    ++    L  F      Q D    
Sbjct: 378 LQGHISFPLVLDLLQFRTTEVGIKSWEENM--KRGQVQRQCRNLGTFHNQSSMQHDQIKR 437

Query: 426 KYINGLTGEDEFPMNLTSGEPICYDRFKGSVEETSSSQTRGSSETWDTNFNSCSSSSETR 485
            +I G      +P  L S   +     +    E+S SQ  G S++   + N  S    T 
Sbjct: 438 NHIYGSMRGSIYPQELASDVSLFTANAQDFSGESSFSQAAGISKSMHADMNLHSEDQLTE 497

Query: 486 TCQIEPSETFLYRVVSVVQHFGKPGGGHYTVYRRVQDYLLGQD--CDTQSQDVSTEWFHV 545
           +C++ PS T LYRVV+VV+HFG+ G GHYTVYR V+  L  ++   +T+    +  WF +
Sbjct: 498 SCEVVPSNTILYRVVAVVEHFGRAGSGHYTVYRSVRADLCDKEPSDETEFDAAALCWFSI 557

Query: 546 SDSEVHLVSEEEVLAAEATMLFYEK 559
           SDS+V  VS E+VLAAEA+ LFYEK
Sbjct: 558 SDSQVCAVSMEDVLAAEASFLFYEK 580

BLAST of CSPI02G15290 vs. NCBI nr
Match: gi|694410779|ref|XP_009333781.1| (PREDICTED: LOW QUALITY PROTEIN: ubiquitin carboxyl-terminal hydrolase 27-like [Pyrus x bretschneideri])

HSP 1 Score: 531.6 bits (1368), Expect = 1.7e-147
Identity = 300/573 (52.36%), Postives = 372/573 (64.92%), Query Frame = 1

Query: 6   ENFITRFQPAEFCVYAAGLLSAAALVSSIRVGKLNILGLLS--WT--KGPSSSENLRLLP 65
           + F+T    +     AA L  A   V ++R  K +IL  L   W      SS E L L+P
Sbjct: 18  QGFMTLSGSSSISSVAAILGVAGGFVLALRGSKFSILNTLDLEWLLHTETSSPEQLPLVP 77

Query: 66  GLQNLGNNCFFNVILQALASCVFFQPFLQKFVEEFELHE-GMRSEALPLTVSLAALLEEL 125
           GLQNLGNNCF NVIL ALASC  FQPFLQK +EE+E    G R +  PL ++LA LLE+L
Sbjct: 78  GLQNLGNNCFLNVILXALASCSCFQPFLQKVLEEYEFSAAGNRVDGFPLAIALATLLEDL 137

Query: 126 GEISLERVVLSPRKVMLAMAYYTPNFSLTSQQDAAEAFLHLLSSLKEEISDFNCPAQCSL 185
             +   RVVLSPRKVMLAMA Y  NF LTSQ DAAEAFLHLLSSL+EE SD   P QCSL
Sbjct: 138 CIVGGGRVVLSPRKVMLAMADYIQNFDLTSQHDAAEAFLHLLSSLREEFSDCYLPNQCSL 197

Query: 186 TDVMGSNGRIITSCN-ESPTELERWQGHFLVPFDGILGSILTCQSCSSQISLSFESFHNL 245
            ++  SN RIIT  + E+ ++  RWQ H+L PFDGILGSILTCQSCSSQISL+FESFH+L
Sbjct: 198 AELCASNCRIITPKSMENXSKQARWQQHYLGPFDGILGSILTCQSCSSQISLNFESFHSL 257

Query: 246 PVSPALRGDSTIVFGCTLLDCLKQFFAAEKVENYRCSYCWHNAGLKYLSSMGASEMEIEE 305
           P+SP L G  TI+ GCTL DCL+QF AAE+VENY C++CWH A +KYL S+G +E EIE+
Sbjct: 258 PLSPVLDGGGTIMVGCTLEDCLEQFTAAEQVENYHCNHCWHIAAVKYLFSLGVNEEEIEK 317

Query: 306 LMKCDRQDSCGCQRLLHLYKVPWSNKFSFTLKQLSIAHCPKILCIHLKRVSTNMFGDAFK 365
           + +C  QDSC C+   HL+K+PWSN+FS+TLKQ+SIA CPKILCIHLKRVS N+FG+  K
Sbjct: 318 IKRCPEQDSCDCRSHFHLHKLPWSNRFSYTLKQISIARCPKILCIHLKRVSVNIFGELVK 377

Query: 366 LQGHISFPLILDMSPFMLTGVEIKDLEGQLGGQHSSNKKPSHGLDFFRT----QFDARML 425
           LQGHISFPL+LD+  F  T V IK  E  +  +    ++    L  F      Q D    
Sbjct: 378 LQGHISFPLVLDLLQFRTTEVGIKSWEENM--KRGQVQRQCRNLGTFHNQSSMQHDQIKR 437

Query: 426 KYINGLTGEDEFPMNLTSGEPICYDRFKGSVE----ETSSSQTRGSSETWDTNFNSCSSS 485
            +I G T    +P  L S       RF  + +    E+S SQ  G S++   + N  S  
Sbjct: 438 NHIYGSTRGSIYPKELASD----VSRFTANAQDFSGESSFSQAAGISKSMHADMNLHSED 497

Query: 486 SETRTCQIEPSETFLYRVVSVVQHFGKPGGGHYTVYRRVQDYLLGQDCDTQSQDVSTE-- 545
             T +C++ PS T LYRVV++V+HFG+ G GHYTVYR V+  L    CD +  D S    
Sbjct: 498 QLTESCEVVPSNTSLYRVVAIVEHFGRAGSGHYTVYRSVRADL----CDKEPSDESEFDA 557

Query: 546 ----WFHVSDSEVHLVSEEEVLAAEATMLFYEK 559
               WF +SDS+V  VS E++LAAEA+ LFYEK
Sbjct: 558 AALCWFSISDSQVCAVSMEDILAAEASFLFYEK 580

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
UBP27_ARATH5.4e-9350.13Ubiquitin carboxyl-terminal hydrolase 27 OS=Arabidopsis thaliana GN=UBP27 PE=2 S... [more]
UBP30_XENTR1.3e-2525.10Ubiquitin carboxyl-terminal hydrolase 30 OS=Xenopus tropicalis GN=usp30 PE=2 SV=... [more]
UBP30_HUMAN5.1e-1925.75Ubiquitin carboxyl-terminal hydrolase 30 OS=Homo sapiens GN=USP30 PE=1 SV=1[more]
UBP30_RAT1.5e-1826.96Ubiquitin carboxyl-terminal hydrolase 30 OS=Rattus norvegicus GN=Usp30 PE=3 SV=1[more]
UBP30_MOUSE1.9e-1826.96Ubiquitin carboxyl-terminal hydrolase 30 OS=Mus musculus GN=Usp30 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LJP9_CUCSA0.0e+0099.46Uncharacterized protein OS=Cucumis sativus GN=Csa_2G298270 PE=3 SV=1[more]
M5WI21_PRUPE8.2e-14953.33Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa023910mg PE=3 SV=1[more]
A0A061FAI5_THECC2.9e-14653.28Ubiquitin carboxyl-terminal hydrolase, putative isoform 1 OS=Theobroma cacao GN=... [more]
A0A061F9Z2_THECC7.2e-14553.19Ubiquitin carboxyl-terminal hydrolase, putative isoform 2 OS=Theobroma cacao GN=... [more]
A0A0B0N7I0_GOSAR2.2e-13851.37Ubiquitin carboxyl-terminal hydrolase 27-like protein OS=Gossypium arboreum GN=F... [more]
Match NameE-valueIdentityDescription
AT4G39370.38.2e-9248.76 ubiquitin-specific protease 27[more]
AT5G10790.12.9e-1222.86 ubiquitin-specific protease 22[more]
AT2G32780.11.9e-0843.24 ubiquitin-specific protease 1[more]
AT1G32850.12.5e-0831.25 ubiquitin-specific protease 11[more]
AT1G04860.15.7e-0841.89 ubiquitin-specific protease 2[more]
Match NameE-valueIdentityDescription
gi|449461457|ref|XP_004148458.1|0.0e+0099.46PREDICTED: ubiquitin carboxyl-terminal hydrolase 27 [Cucumis sativus][more]
gi|659116134|ref|XP_008457921.1|0.0e+0096.06PREDICTED: ubiquitin carboxyl-terminal hydrolase 27 [Cucumis melo][more]
gi|595838648|ref|XP_007207694.1|1.2e-14853.33hypothetical protein PRUPE_ppa023910mg [Prunus persica][more]
gi|694430671|ref|XP_009342800.1|1.0e-14752.39PREDICTED: ubiquitin carboxyl-terminal hydrolase 27-like [Pyrus x bretschneideri... [more]
gi|694410779|ref|XP_009333781.1|1.7e-14752.36PREDICTED: LOW QUALITY PROTEIN: ubiquitin carboxyl-terminal hydrolase 27-like [P... [more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR001394Peptidase_C19_UCH
IPR018200USP_CS
Vocabulary: Biological Process
TermDefinition
GO:0016579protein deubiquitination
GO:0006511ubiquitin-dependent protein catabolic process
Vocabulary: Molecular Function
TermDefinition
GO:0036459thiol-dependent ubiquitinyl hydrolase activity
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016579 protein deubiquitination
biological_process GO:0006511 ubiquitin-dependent protein catabolic process
cellular_component GO:0005575 cellular_component
cellular_component GO:0031307 integral component of mitochondrial outer membrane
molecular_function GO:0036459 thiol-dependent ubiquitinyl hydrolase activity
molecular_function GO:0004843 thiol-dependent ubiquitin-specific protease activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI02G15290.1CSPI02G15290.1mRNA


Analysis Name: InterPro Annotations of cucumber (PI183967)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001394Peptidase C19, ubiquitin carboxyl-terminal hydrolasePFAMPF00443UCHcoord: 62..556
score: 1.9
IPR018200Ubiquitin specific protease, conserved sitePROSITEPS00972USP_1coord: 62..77
scor
NoneNo IPR availablePANTHERPTHR24006FAMILY NOT NAMEDcoord: 479..559
score: 5.7E-97coord: 329..378
score: 5.7E-97coord: 62..283
score: 5.7
NoneNo IPR availablePANTHERPTHR24006:SF455UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 27coord: 329..378
score: 5.7E-97coord: 62..283
score: 5.7E-97coord: 479..559
score: 5.7
NoneNo IPR availableunknownSSF54001Cysteine proteinasescoord: 482..558
score: 6.28E-68coord: 324..379
score: 6.28E-68coord: 60..278
score: 6.28

The following gene(s) are paralogous to this gene:

None